AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024979


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024979.t1 MTR_3g103020 61.929 197 69 4 17 210 185 378 1.19e-79 243
MS.gene024979.t1 MTR_4g109270 61.463 205 75 4 7 210 163 364 4.21e-79 241
MS.gene024979.t1 MTR_6g060650 62.051 195 69 4 15 209 183 372 7.92e-79 240
MS.gene024979.t1 MTR_4g109280 60.714 196 70 5 17 210 169 359 4.95e-75 230
MS.gene024979.t1 MTR_4g117720 60.194 206 71 6 12 210 177 378 9.27e-75 230
MS.gene024979.t1 MTR_4g116330 54.808 208 89 3 5 210 176 380 4.68e-73 226
MS.gene024979.t1 MTR_8g040935 56.122 196 82 3 18 210 14 208 5.72e-71 215
MS.gene024979.t1 MTR_3g034180 58.706 201 72 5 15 209 293 488 1.18e-70 223
MS.gene024979.t1 MTR_3g034160 59.406 202 69 6 15 209 179 374 9.65e-69 214
MS.gene024979.t1 MTR_4g117870 56.701 194 45 3 17 210 113 267 3.46e-68 210
MS.gene024979.t1 MTR_4g023130 56.633 196 77 5 15 209 124 312 8.45e-67 208
MS.gene024979.t1 MTR_6g463430 55.385 195 79 4 17 210 15 202 2.92e-65 200
MS.gene024979.t1 MTR_4g117850 56.410 195 80 5 16 207 184 376 3.03e-65 206
MS.gene024979.t1 MTR_3g034130 55.319 188 74 4 15 196 179 362 5.74e-65 205
MS.gene024979.t1 MTR_4g117770 53.623 207 91 3 7 210 163 367 1.49e-64 204
MS.gene024979.t1 MTR_4g117810 53.623 207 91 3 7 210 163 367 1.49e-64 204
MS.gene024979.t1 MTR_4g023080 54.592 196 81 5 15 209 165 353 1.71e-64 203
MS.gene024979.t1 MTR_4g117780 52.657 207 93 3 7 210 163 367 5.76e-64 202
MS.gene024979.t1 MTR_5g023000 92.929 99 7 0 112 210 1 99 1.96e-63 192
MS.gene024979.t1 MTR_8g433030 53.061 196 87 4 11 205 146 337 2.42e-63 200
MS.gene024979.t1 MTR_4g116320 55.385 195 80 4 15 207 66 255 3.11e-63 197
MS.gene024979.t1 MTR_8g032390 53.061 196 87 4 11 205 179 370 7.61e-63 199
MS.gene024979.t1 MTR_4g023070 53.398 206 77 5 17 209 170 369 1.59e-62 199
MS.gene024979.t1 MTR_4g021785 54.082 196 85 4 15 210 165 355 1.73e-62 198
MS.gene024979.t1 MTR_4g094365 51.220 205 94 4 8 210 164 364 3.94e-62 197
MS.gene024979.t1 MTR_4g023090 51.196 209 82 5 15 209 181 383 1.10e-61 197
MS.gene024979.t1 MTR_4g122850 52.500 200 87 7 15 210 169 364 3.71e-60 193
MS.gene024979.t1 MTR_4g023120 50.467 214 87 5 10 210 163 370 6.77e-60 192
MS.gene024979.t1 MTR_4g118380 59.195 174 57 5 17 181 187 355 1.63e-59 191
MS.gene024979.t1 MTR_4g023380 49.541 218 80 6 17 210 168 379 2.72e-58 188
MS.gene024979.t1 MTR_5g097210 52.604 192 84 5 17 206 173 359 2.55e-57 185
MS.gene024979.t1 MTR_5g070080 53.333 195 87 4 17 210 195 386 8.28e-57 184
MS.gene024979.t1 MTR_8g433040 49.057 212 91 5 1 210 168 364 3.36e-56 182
MS.gene024979.t1 MTR_8g032380 49.057 212 91 5 1 210 168 364 3.36e-56 182
MS.gene024979.t1 MTR_1g055175 50.000 206 93 8 10 210 1 201 5.50e-56 176
MS.gene024979.t1 MTR_4g118400 51.942 206 79 6 2 204 106 294 2.65e-55 178
MS.gene024979.t1 MTR_5g070130 49.505 202 93 5 17 210 177 377 9.84e-55 179
MS.gene024979.t1 MTR_4g023200 52.000 200 82 7 15 208 167 358 3.97e-54 177
MS.gene024979.t1 MTR_4g023190 53.030 198 82 6 15 208 167 357 1.82e-53 175
MS.gene024979.t1 MTR_4g023150 50.476 210 93 7 4 208 155 358 6.62e-53 173
MS.gene024979.t1 MTR_1g055195 49.261 203 88 9 17 210 282 478 1.45e-52 176
MS.gene024979.t1 MTR_4g116330 46.154 208 68 3 5 210 176 341 5.42e-52 171
MS.gene024979.t1 MTR_5g070088 48.980 196 96 4 17 210 193 386 4.56e-50 167
MS.gene024979.t1 MTR_4g118370 48.205 195 75 6 16 207 86 257 8.14e-49 160
MS.gene024979.t1 MTR_1g055135 47.980 198 92 7 20 210 182 375 3.70e-48 162
MS.gene024979.t1 MTR_4g117530 46.907 194 67 4 17 210 96 253 6.47e-48 157
MS.gene024979.t1 MTR_1g055250 48.925 186 81 8 17 197 77 253 2.12e-47 158
MS.gene024979.t1 MTR_1g055165 51.786 168 74 6 45 210 156 318 3.77e-46 155
MS.gene024979.t1 MTR_4g117750 49.254 201 84 9 17 206 162 355 2.05e-45 157
MS.gene024979.t1 MTR_4g117750 56.061 132 50 1 85 208 350 481 3.49e-38 137
MS.gene024979.t1 MTR_4g023440 45.500 200 62 4 13 210 178 332 6.42e-44 149
MS.gene024979.t1 MTR_7g079400 44.231 208 100 8 13 210 136 337 1.75e-42 146
MS.gene024979.t1 MTR_8g102520 41.748 206 108 8 11 210 123 322 2.83e-42 145
MS.gene024979.t1 MTR_4g118360 63.793 116 36 4 97 210 15 126 9.06e-41 135
MS.gene024979.t1 MTR_4g122860 42.188 192 77 5 24 210 158 320 1.46e-38 136
MS.gene024979.t1 MTR_7g079370 45.026 191 96 7 18 204 165 350 1.18e-37 134
MS.gene024979.t1 MTR_4g121580 44.211 190 71 6 14 200 182 339 5.69e-36 130
MS.gene024979.t1 MTR_8g098830 46.154 169 79 8 18 181 35 196 7.30e-36 126
MS.gene024979.t1 MTR_7g079450 46.853 143 69 4 69 210 1 137 5.17e-31 110
MS.gene024979.t1 MTR_2g048580 39.394 198 102 8 18 210 158 342 5.98e-30 113
MS.gene024979.t1 MTR_4g114700 38.503 187 63 3 18 201 81 218 5.86e-28 105
MS.gene024979.t1 MTR_8g013010 55.660 106 39 5 11 115 217 315 1.18e-27 107
MS.gene024979.t1 MTR_4g117430 51.887 106 46 4 7 112 55 155 3.80e-27 101
MS.gene024979.t1 MTR_7g079420 38.251 183 100 7 35 210 968 1144 7.73e-27 107
MS.gene024979.t1 MTR_7g117720 63.218 87 28 2 123 205 186 272 1.15e-25 100
MS.gene024979.t1 MTR_4g122840 40.441 136 45 3 76 210 164 264 9.06e-22 90.1
MS.gene024979.t1 MTR_8g013005 61.017 59 23 0 152 210 1 59 5.02e-19 77.8
MS.gene024979.t1 MTR_4g023430 50.000 82 38 1 132 210 141 222 2.90e-18 80.1
MS.gene024979.t1 MTR_4g133170 28.571 189 124 7 17 201 173 354 4.87e-13 67.4
MS.gene024979.t1 MTR_0129s0050 27.604 192 125 4 19 205 224 406 7.32e-13 67.0
MS.gene024979.t1 MTR_4g023100 57.143 56 22 1 154 209 65 118 8.02e-13 63.2
MS.gene024979.t1 MTR_4g109290 58.491 53 22 0 158 210 136 188 1.78e-12 63.9
MS.gene024979.t1 MTR_2g426020 27.941 204 124 9 17 207 190 383 3.14e-12 65.1
MS.gene024979.t1 MTR_2g028130 30.120 166 103 6 17 175 194 353 7.20e-12 63.9
MS.gene024979.t1 MTR_8g099460 28.846 156 96 5 17 170 184 326 1.20e-11 63.2
MS.gene024979.t1 MTR_2g026010 27.919 197 114 9 17 205 169 345 1.24e-11 63.2
MS.gene024979.t1 MTR_2g425980 30.542 203 121 8 17 207 189 383 1.44e-11 63.2
MS.gene024979.t1 MTR_4g116340 56.897 58 22 3 18 75 193 247 1.82e-11 62.0
MS.gene024979.t1 MTR_2g025420 27.451 204 122 8 17 202 197 392 2.42e-11 62.4
MS.gene024979.t1 MTR_2g028140 33.858 127 69 6 17 135 198 317 4.00e-11 62.0
MS.gene024979.t1 MTR_3g083010 27.143 210 130 8 17 210 199 401 8.58e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAATTATTTCTATGGTTT+TGG 0.137317 5.3:+381954 MS.gene024979:CDS
AATTGTGATGTGTGGGTATT+TGG 0.202909 5.3:+382287 MS.gene024979:CDS
TTGGAGAATTTCTCAGTATA+TGG 0.237144 5.3:+382306 MS.gene024979:CDS
TCGTATCGAAATTATTTCTA+TGG 0.245541 5.3:+381948 MS.gene024979:CDS
CTAATGATACACTTGAAATA+TGG 0.280671 5.3:+382342 MS.gene024979:CDS
ATTAGCTAATACTAAGCATT+TGG 0.343978 5.3:+382031 MS.gene024979:intron
TCAGTAGAGGAATTGATATA+AGG 0.357380 5.3:-382107 None:intergenic
GCTTCATAGTCAATATCAAT+TGG 0.392804 5.3:-382260 None:intergenic
ACTGATTACCCCAAAATAAC+AGG 0.399631 5.3:+382125 MS.gene024979:CDS
TGGAGAATTTCTCAGTATAT+GGG 0.404173 5.3:+382307 MS.gene024979:CDS
TGCCTTTGATTTAATCAAAA+GGG 0.415367 5.3:+382217 MS.gene024979:CDS
TTGCCTTTGATTTAATCAAA+AGG 0.422457 5.3:+382216 MS.gene024979:CDS
AACAGAAGATGGATGGAAAT+TGG 0.423122 5.3:+382484 MS.gene024979:CDS
CAGTAGAGGAATTGATATAA+GGG 0.438574 5.3:-382106 None:intergenic
GGTGACAGTGACCAAGCTTA+AGG 0.452041 5.3:+382611 MS.gene024979:CDS
GAGTCTCTGCTTTCACTCCC+TGG 0.452167 5.3:+382590 MS.gene024979:CDS
AATACAAAGTGCATTCCTCT+TGG 0.461402 5.3:+382375 MS.gene024979:CDS
ATTTCTCAGTATATGGGCTA+TGG 0.479112 5.3:+382313 MS.gene024979:CDS
GATATCATTGGAACAGAAGA+TGG 0.479823 5.3:+382473 MS.gene024979:CDS
TATTGGGGAAAACAGCTTAG+TGG 0.491914 5.3:-382432 None:intergenic
TTGTGTCATCTCCTTAAGCT+TGG 0.494818 5.3:-382622 None:intergenic
TAATGATACACTTGAAATAT+GGG 0.496908 5.3:+382343 MS.gene024979:CDS
CTTCATAGTCAATATCAATT+GGG 0.498545 5.3:-382259 None:intergenic
TAAGCTTGGTCACTGTCACC+AGG 0.500905 5.3:-382608 None:intergenic
TAATACATGGAAAGAAATTG+AGG 0.507096 5.3:+382073 MS.gene024979:CDS
TCTCATTCAGAGCTAATACA+TGG 0.515391 5.3:+382060 MS.gene024979:CDS
CTCTGTATAAAGAGCCACTT+CGG 0.548132 5.3:-382570 None:intergenic
TCGTCACGATCATTTACGAA+TGG 0.560012 5.3:+382184 MS.gene024979:CDS
AATACATGGAAAGAAATTGA+GGG 0.567862 5.3:+382074 MS.gene024979:CDS
TCATTGGAACAGAAGATGGA+TGG 0.571754 5.3:+382477 MS.gene024979:CDS
TACTATTCTGACAGTACACA+TGG 0.588984 5.3:+382545 MS.gene024979:CDS
AAGCTTGGTCACTGTCACCA+GGG 0.590911 5.3:-382607 None:intergenic
AGCTACAAATTGTGATGTGT+GGG 0.604681 5.3:+382280 MS.gene024979:CDS
AAGCTACAAATTGTGATGTG+TGG 0.622283 5.3:+382279 MS.gene024979:CDS
TTGGTGAAGTATAATGACAA+AGG 0.632256 5.3:+382503 MS.gene024979:CDS
ACAAAGGACAGTTGATAGAG+TGG 0.633105 5.3:+382519 MS.gene024979:CDS
CAGTACACATGGAACCGAAG+TGG 0.667775 5.3:+382556 MS.gene024979:CDS
ATAGAAATAATTTCGATACG+AGG 0.746208 5.3:-381946 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGATACACTTGAAATAT+GGG + chr5.3:382343-382362 MS.gene024979:CDS 20.0%
!!! GAAATTATTTCTATGGTTTT+GGG + chr5.3:381955-381974 MS.gene024979:CDS 20.0%
! AATACATGGAAAGAAATTGA+GGG + chr5.3:382074-382093 MS.gene024979:CDS 25.0%
! ATAGAAATAATTTCGATACG+AGG - chr5.3:381949-381968 None:intergenic 25.0%
! ATGCTTAGTATTAGCTAATT+TGG - chr5.3:382030-382049 None:intergenic 25.0%
! ATTAGCTAATACTAAGCATT+TGG + chr5.3:382031-382050 MS.gene024979:intron 25.0%
! CTAATGATACACTTGAAATA+TGG + chr5.3:382342-382361 MS.gene024979:CDS 25.0%
! CTTCATAGTCAATATCAATT+GGG - chr5.3:382262-382281 None:intergenic 25.0%
! TAATACATGGAAAGAAATTG+AGG + chr5.3:382073-382092 MS.gene024979:CDS 25.0%
! TGCTTAGTATTAGCTAATTT+GGG - chr5.3:382029-382048 None:intergenic 25.0%
!! ACTTTTTGTAGAACATATTG+GGG - chr5.3:382450-382469 None:intergenic 25.0%
!! TGCCTTTGATTTAATCAAAA+GGG + chr5.3:382217-382236 MS.gene024979:CDS 25.0%
!! TTGCCTTTGATTTAATCAAA+AGG + chr5.3:382216-382235 MS.gene024979:CDS 25.0%
!!! CACTTTTTGTAGAACATATT+GGG - chr5.3:382451-382470 None:intergenic 25.0%
!!! CGAAATTATTTCTATGGTTT+TGG + chr5.3:381954-381973 MS.gene024979:CDS 25.0%
!!! TCGTATCGAAATTATTTCTA+TGG + chr5.3:381948-381967 MS.gene024979:CDS 25.0%
!!! TTCCCTTTTGATTAAATCAA+AGG - chr5.3:382222-382241 None:intergenic 25.0%
ACAAAAAGTGGTGATATCAT+TGG + chr5.3:382461-382480 MS.gene024979:CDS 30.0%
CAGTAGAGGAATTGATATAA+GGG - chr5.3:382109-382128 None:intergenic 30.0%
CCAATATGTTCTACAAAAAG+TGG + chr5.3:382449-382468 MS.gene024979:CDS 30.0%
GCTTAGTATTAGCTAATTTG+GGG - chr5.3:382028-382047 None:intergenic 30.0%
GCTTCATAGTCAATATCAAT+TGG - chr5.3:382263-382282 None:intergenic 30.0%
TCAGTAGAGGAATTGATATA+AGG - chr5.3:382110-382129 None:intergenic 30.0%
TGGAGAATTTCTCAGTATAT+GGG + chr5.3:382307-382326 MS.gene024979:CDS 30.0%
TTGGAGAATTTCTCAGTATA+TGG + chr5.3:382306-382325 MS.gene024979:CDS 30.0%
TTGGTGAAGTATAATGACAA+AGG + chr5.3:382503-382522 MS.gene024979:CDS 30.0%
! ATAACAGGCTCTCTTTTTAA+TGG + chr5.3:382140-382159 MS.gene024979:CDS 30.0%
! TAACAGGCTCTCTTTTTAAT+GGG + chr5.3:382141-382160 MS.gene024979:CDS 30.0%
! TTTTTAATGGGGCTATTCAT+TGG + chr5.3:382153-382172 MS.gene024979:CDS 30.0%
!! AAAAAGAGAGCCTGTTATTT+TGG - chr5.3:382138-382157 None:intergenic 30.0%
!!! AAAAGAGAGCCTGTTATTTT+GGG - chr5.3:382137-382156 None:intergenic 30.0%
!!! ACTCTTGTTTTGTCTATTGA+CGG + chr5.3:382404-382423 MS.gene024979:CDS 30.0%
!!! CCACTTTTTGTAGAACATAT+TGG - chr5.3:382452-382471 None:intergenic 30.0%
AACAGAAGATGGATGGAAAT+TGG + chr5.3:382484-382503 MS.gene024979:CDS 35.0%
AAGCTACAAATTGTGATGTG+TGG + chr5.3:382279-382298 MS.gene024979:CDS 35.0%
AATACAAAGTGCATTCCTCT+TGG + chr5.3:382375-382394 MS.gene024979:CDS 35.0%
AATTGTGATGTGTGGGTATT+TGG + chr5.3:382287-382306 MS.gene024979:CDS 35.0%
ACTGATTACCCCAAAATAAC+AGG + chr5.3:382125-382144 MS.gene024979:CDS 35.0%
AGCTACAAATTGTGATGTGT+GGG + chr5.3:382280-382299 MS.gene024979:CDS 35.0%
GATATCATTGGAACAGAAGA+TGG + chr5.3:382473-382492 MS.gene024979:CDS 35.0%
GTCAACTGATGATTACTTCT+TGG + chr5.3:381986-382005 MS.gene024979:intron 35.0%
TACTATTCTGACAGTACACA+TGG + chr5.3:382545-382564 MS.gene024979:CDS 35.0%
TCTCATTCAGAGCTAATACA+TGG + chr5.3:382060-382079 MS.gene024979:CDS 35.0%
! AACAAGAGTTTTTGTCCAAG+AGG - chr5.3:382393-382412 None:intergenic 35.0%
! AACAGGCTCTCTTTTTAATG+GGG + chr5.3:382142-382161 MS.gene024979:CDS 35.0%
! ATTTCTCAGTATATGGGCTA+TGG + chr5.3:382313-382332 MS.gene024979:CDS 35.0%
!!! AAAGAGAGCCTGTTATTTTG+GGG - chr5.3:382136-382155 None:intergenic 35.0%
!!! TATTTTGGGGTAATCAGTAG+AGG - chr5.3:382123-382142 None:intergenic 35.0%
!!! TTTCTATGGTTTTGGGTATG+AGG + chr5.3:381962-381981 MS.gene024979:intron 35.0%
CTCTGTATAAAGAGCCACTT+CGG - chr5.3:382573-382592 None:intergenic 40.0%
TATTGGGGAAAACAGCTTAG+TGG - chr5.3:382435-382454 None:intergenic 40.0%
TCATTGGAACAGAAGATGGA+TGG + chr5.3:382477-382496 MS.gene024979:CDS 40.0%
TCGTCACGATCATTTACGAA+TGG + chr5.3:382184-382203 MS.gene024979:CDS 40.0%
!! ACAAAGGACAGTTGATAGAG+TGG + chr5.3:382519-382538 MS.gene024979:CDS 40.0%
AAGCTTGGTCACTGTCACCA+GGG - chr5.3:382610-382629 None:intergenic 50.0%
CAGTACACATGGAACCGAAG+TGG + chr5.3:382556-382575 MS.gene024979:CDS 50.0%
TAAGCTTGGTCACTGTCACC+AGG - chr5.3:382611-382630 None:intergenic 50.0%
GAGTCTCTGCTTTCACTCCC+TGG + chr5.3:382590-382609 MS.gene024979:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 381940 382631 381940 ID=MS.gene024979
chr5.3 mRNA 381940 382631 381940 ID=MS.gene024979.t1;Parent=MS.gene024979
chr5.3 exon 381940 381976 381940 ID=MS.gene024979.t1.exon1;Parent=MS.gene024979.t1
chr5.3 CDS 381940 381976 381940 ID=cds.MS.gene024979.t1;Parent=MS.gene024979.t1
chr5.3 exon 382036 382631 382036 ID=MS.gene024979.t1.exon2;Parent=MS.gene024979.t1
chr5.3 CDS 382036 382631 382036 ID=cds.MS.gene024979.t1;Parent=MS.gene024979.t1
Gene Sequence

>MS.gene024979

ATGGTTCCTCGTATCGAAATTATTTCTATGGTTTTGGGTATGAGGAGTCAACTGATGATTACTTCTTGGTGTCAATTTCTCATGACCCCAAATTAGCTAATACTAAGCATTTGGAGATTTTCTCATTCAGAGCTAATACATGGAAAGAAATTGAGGGTATTCACTGCCCTTATATCAATTCCTCTACTGATTACCCCAAAATAACAGGCTCTCTTTTTAATGGGGCTATTCATTGGTTCGCTTTTCGTCACGATCATTTACGAATGGATCTTATTCTTGCCTTTGATTTAATCAAAAGGGAACTTTTTGAGATTCATCTCCCAATTGATATTGACTATGAAGCTACAAATTGTGATGTGTGGGTATTTGGAGAATTTCTCAGTATATGGGCTATGGATTATGCTAATGATACACTTGAAATATGGGTGATGAAAGAATACAAAGTGCATTCCTCTTGGACAAAAACTCTTGTTTTGTCTATTGACGGCATTTCCACTAAGCTGTTTTCCCCAATATGTTCTACAAAAAGTGGTGATATCATTGGAACAGAAGATGGATGGAAATTGGTGAAGTATAATGACAAAGGACAGTTGATAGAGTGGCACTACTATTCTGACAGTACACATGGAACCGAAGTGGCTCTTTATACAGAGTCTCTGCTTTCACTCCCTGGTGACAGTGACCAAGCTTAA

Protein sequence

>MS.gene024979.t1

MVPRIEIISMVLANTKHLEIFSFRANTWKEIEGIHCPYINSSTDYPKITGSLFNGAIHWFAFRHDHLRMDLILAFDLIKRELFEIHLPIDIDYEATNCDVWVFGEFLSIWAMDYANDTLEIWVMKEYKVHSSWTKTLVLSIDGISTKLFSPICSTKSGDIIGTEDGWKLVKYNDKGQLIEWHYYSDSTHGTEVALYTESLLSLPGDSDQA