AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064108

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064108.t1 MTR_4g023080 82.770 296 46 2 1 295 62 353 2.97e-175 488
MS.gene064108.t1 MTR_4g021785 81.544 298 49 2 1 296 62 355 1.30e-172 481
MS.gene064108.t1 MTR_4g023130 77.818 275 56 2 22 295 42 312 3.73e-148 418
MS.gene064108.t1 MTR_4g023090 68.489 311 78 4 1 291 69 379 1.24e-145 414
MS.gene064108.t1 MTR_4g023380 66.770 322 70 6 1 296 69 379 7.77e-140 399
MS.gene064108.t1 MTR_4g023070 60.063 318 100 4 1 295 56 369 6.29e-127 367
MS.gene064108.t1 MTR_4g023120 59.561 319 102 4 1 296 56 370 1.59e-125 363
MS.gene064108.t1 MTR_4g023200 60.535 299 110 4 1 294 63 358 6.50e-117 340
MS.gene064108.t1 MTR_4g023150 60.269 297 110 4 3 294 65 358 9.40e-115 335
MS.gene064108.t1 MTR_4g023190 59.933 297 110 5 3 294 65 357 2.85e-113 331
MS.gene064108.t1 MTR_4g023440 59.468 301 77 4 1 296 72 332 4.00e-111 325
MS.gene064108.t1 MTR_3g103020 56.106 303 115 7 9 296 79 378 6.98e-103 305
MS.gene064108.t1 MTR_4g117720 51.148 305 130 7 9 296 76 378 4.75e-94 283
MS.gene064108.t1 MTR_4g094365 53.872 297 125 7 10 296 70 364 1.31e-92 279
MS.gene064108.t1 MTR_4g109270 52.303 304 132 6 3 296 64 364 1.43e-92 279
MS.gene064108.t1 MTR_4g122850 54.072 307 121 11 7 296 61 364 8.64e-91 275
MS.gene064108.t1 MTR_3g034180 52.532 316 122 9 3 295 178 488 1.22e-89 275
MS.gene064108.t1 MTR_4g109280 51.815 303 122 9 9 296 66 359 2.29e-88 268
MS.gene064108.t1 MTR_6g060650 48.860 307 135 7 9 295 68 372 1.92e-86 263
MS.gene064108.t1 MTR_8g433040 51.342 298 123 5 10 296 78 364 2.62e-85 260
MS.gene064108.t1 MTR_8g032380 51.342 298 123 5 10 296 78 364 2.62e-85 260
MS.gene064108.t1 MTR_3g034160 51.438 313 126 8 3 293 64 372 1.21e-84 259
MS.gene064108.t1 MTR_3g034130 52.265 287 112 7 9 274 70 352 1.43e-84 258
MS.gene064108.t1 MTR_4g117770 51.792 307 130 9 3 296 66 367 1.54e-84 258
MS.gene064108.t1 MTR_4g117810 51.792 307 130 9 3 296 66 367 1.54e-84 258
MS.gene064108.t1 MTR_4g117780 51.171 299 131 9 11 296 71 367 1.74e-81 250
MS.gene064108.t1 MTR_4g117850 49.007 302 127 9 9 293 85 376 4.75e-80 247
MS.gene064108.t1 MTR_4g118380 46.918 292 124 5 9 271 68 357 4.55e-78 241
MS.gene064108.t1 MTR_4g116330 48.667 300 134 7 12 296 86 380 6.93e-78 242
MS.gene064108.t1 MTR_8g032390 48.475 295 117 8 9 291 99 370 9.31e-74 231
MS.gene064108.t1 MTR_4g117870 47.603 292 104 9 12 292 10 263 2.75e-73 226
MS.gene064108.t1 MTR_5g070130 44.516 310 147 9 9 296 71 377 3.86e-73 230
MS.gene064108.t1 MTR_5g097210 47.896 309 132 11 1 292 63 359 1.04e-71 226
MS.gene064108.t1 MTR_1g055135 43.168 322 141 11 3 296 68 375 2.21e-67 214
MS.gene064108.t1 MTR_5g070088 41.088 331 151 8 1 294 60 383 2.59e-67 215
MS.gene064108.t1 MTR_5g070080 43.168 322 144 11 9 296 70 386 6.45e-66 211
MS.gene064108.t1 MTR_4g116320 55.349 215 80 5 93 293 43 255 9.25e-66 207
MS.gene064108.t1 MTR_4g121580 44.951 307 120 12 7 295 73 348 4.93e-64 206
MS.gene064108.t1 MTR_1g055195 43.919 296 130 11 20 296 200 478 7.68e-62 203
MS.gene064108.t1 MTR_8g433030 53.953 215 85 7 89 291 125 337 1.40e-59 193
MS.gene064108.t1 MTR_4g118400 43.321 277 122 8 24 289 41 293 7.37e-59 190
MS.gene064108.t1 MTR_8g040935 51.724 203 89 4 103 296 6 208 1.90e-56 181
MS.gene064108.t1 MTR_4g117750 41.613 310 137 11 9 292 64 355 5.38e-56 188
MS.gene064108.t1 MTR_4g117750 55.963 109 46 1 188 294 373 481 4.54e-31 121
MS.gene064108.t1 MTR_7g079370 38.889 306 158 9 9 289 48 349 1.01e-55 184
MS.gene064108.t1 MTR_6g463430 54.592 196 78 5 107 296 12 202 3.53e-55 177
MS.gene064108.t1 MTR_4g116330 41.472 299 118 7 12 296 86 341 3.65e-55 182
MS.gene064108.t1 MTR_1g055165 38.983 295 127 8 9 296 70 318 2.20e-52 174
MS.gene064108.t1 MTR_7g079400 40.984 305 146 13 16 296 43 337 3.82e-51 171
MS.gene064108.t1 MTR_4g117530 44.068 236 99 4 64 296 48 253 2.76e-50 167
MS.gene064108.t1 MTR_8g013010 47.475 198 92 4 9 197 115 309 5.20e-50 168
MS.gene064108.t1 MTR_4g118370 41.667 252 104 10 58 293 33 257 2.85e-45 154
MS.gene064108.t1 MTR_4g117430 53.521 142 62 2 57 197 14 152 9.93e-43 144
MS.gene064108.t1 MTR_8g102520 37.908 306 141 12 9 296 48 322 1.31e-42 149
MS.gene064108.t1 MTR_1g055250 38.961 231 113 8 77 283 27 253 2.30e-41 145
MS.gene064108.t1 MTR_4g114700 38.258 264 101 5 42 290 5 221 2.64e-41 143
MS.gene064108.t1 MTR_1g055175 42.347 196 96 7 113 296 11 201 5.81e-39 136
MS.gene064108.t1 MTR_2g048580 36.519 293 141 14 12 289 73 335 8.92e-39 139
MS.gene064108.t1 MTR_7g117720 51.449 138 57 3 7 134 50 187 4.45e-37 133
MS.gene064108.t1 MTR_7g117720 64.706 85 24 2 211 289 186 270 4.92e-23 96.3
MS.gene064108.t1 MTR_7g079420 35.472 265 133 12 45 296 905 1144 4.78e-35 134
MS.gene064108.t1 MTR_5g023000 64.211 95 32 1 204 296 5 99 1.85e-32 115
MS.gene064108.t1 MTR_4g023430 51.908 131 46 2 183 296 92 222 8.81e-32 118
MS.gene064108.t1 MTR_4g116340 43.636 165 81 4 12 164 83 247 6.33e-30 114
MS.gene064108.t1 MTR_4g118360 55.357 112 42 3 186 293 16 123 2.29e-29 108
MS.gene064108.t1 MTR_8g098830 41.520 171 85 7 106 267 30 194 8.50e-28 107
MS.gene064108.t1 MTR_4g122860 40.212 189 78 8 116 295 157 319 4.55e-25 102
MS.gene064108.t1 MTR_7g079450 42.958 142 73 4 158 296 1 137 1.04e-23 94.4
MS.gene064108.t1 MTR_4g117550 29.562 274 141 10 47 294 127 374 1.51e-22 96.3
MS.gene064108.t1 MTR_6g034720 45.378 119 55 3 67 175 51 169 3.19e-22 92.4
MS.gene064108.t1 MTR_4g133170 27.347 245 152 9 39 261 86 326 1.46e-20 90.9
MS.gene064108.t1 MTR_2g021800 26.299 308 179 8 26 289 109 412 2.49e-19 87.8
MS.gene064108.t1 MTR_1g097560 26.370 292 190 12 7 288 97 373 4.17e-19 86.7
MS.gene064108.t1 MTR_4g023100 80.769 52 10 0 242 293 65 116 6.29e-19 80.9
MS.gene064108.t1 MTR_2g022000 26.974 304 174 9 26 285 108 407 7.78e-19 86.3
MS.gene064108.t1 MTR_0129s0050 28.859 298 152 11 47 293 120 408 1.21e-18 85.9
MS.gene064108.t1 MTR_1g097490 27.333 300 181 13 7 288 110 390 1.38e-17 82.4
MS.gene064108.t1 MTR_5g097330 25.714 280 170 7 39 289 129 399 2.39e-17 82.0
MS.gene064108.t1 MTR_5g037680 28.727 275 166 13 34 288 117 381 3.15e-17 82.0
MS.gene064108.t1 MTR_5g097350 26.506 249 156 8 57 289 146 383 4.35e-17 80.9
MS.gene064108.t1 MTR_4g028510 26.667 285 169 8 43 291 120 400 4.75e-17 81.3
MS.gene064108.t1 MTR_1g050542 27.138 269 165 13 41 288 146 404 4.93e-17 80.9
MS.gene064108.t1 MTR_0128s0020 27.857 280 164 11 41 287 118 392 5.31e-16 77.8
MS.gene064108.t1 MTR_6g090180 26.721 247 144 5 56 269 170 412 8.95e-16 77.4
MS.gene064108.t1 MTR_2g426070 29.921 254 141 11 43 264 105 353 1.23e-15 77.0
MS.gene064108.t1 MTR_2g025960 30.085 236 129 10 43 246 5 236 1.99e-15 75.5
MS.gene064108.t1 MTR_0129s0070 26.910 301 177 12 25 289 99 392 2.74e-15 75.9
MS.gene064108.t1 MTR_2g055970 26.515 264 178 9 37 288 301 560 3.18e-15 76.3
MS.gene064108.t1 MTR_2g028130 28.689 244 137 11 43 253 106 345 8.81e-15 74.3
MS.gene064108.t1 MTR_2g025420 29.889 271 139 14 23 249 84 347 1.07e-14 74.3
MS.gene064108.t1 MTR_4g070550 30.233 215 112 10 43 223 101 311 1.14e-14 73.9
MS.gene064108.t1 MTR_5g059430 24.399 291 183 10 34 293 142 426 1.19e-14 73.9
MS.gene064108.t1 MTR_2g026010 28.627 255 152 11 39 265 86 338 1.42e-14 73.9
MS.gene064108.t1 MTR_6g021880 25.253 297 193 11 15 288 91 381 1.76e-14 73.6
MS.gene064108.t1 MTR_7g070880 28.625 269 125 14 41 249 114 375 1.90e-14 73.6
MS.gene064108.t1 MTR_2g426090 29.237 236 131 10 43 246 100 331 2.78e-14 72.8
MS.gene064108.t1 MTR_8g063440 25.000 276 175 9 39 289 111 379 3.98e-14 72.4
MS.gene064108.t1 MTR_8g464770 26.351 296 174 12 24 289 95 376 5.73e-14 72.0
MS.gene064108.t1 MTR_2g021770 28.344 314 173 14 25 293 101 407 6.37e-14 72.0
MS.gene064108.t1 MTR_1g055200 49.351 77 30 3 42 115 5 75 7.45e-14 67.0
MS.gene064108.t1 MTR_8g013005 63.158 57 19 1 242 296 3 59 7.56e-14 65.5
MS.gene064108.t1 MTR_6g080680 23.630 292 189 9 30 292 108 394 1.03e-13 71.2
MS.gene064108.t1 MTR_8g101580 25.887 282 181 11 29 288 119 394 1.11e-13 71.2
MS.gene064108.t1 MTR_2g028140 28.111 217 122 8 39 223 103 317 1.16e-13 71.2
MS.gene064108.t1 MTR_1g068835 27.376 263 168 9 24 265 72 332 1.34e-13 70.9
MS.gene064108.t1 MTR_8g012090 27.273 231 140 8 39 261 121 331 1.47e-13 70.5
MS.gene064108.t1 MTR_5g069120 39.806 103 57 1 9 106 52 154 1.83e-13 67.8
MS.gene064108.t1 MTR_2g066170 28.319 226 129 7 57 258 133 349 2.21e-13 70.1
MS.gene064108.t1 MTR_0044s0300 28.409 264 158 14 44 288 138 389 3.15e-13 69.7
MS.gene064108.t1 MTR_8g039500 28.958 259 139 13 30 258 97 340 3.66e-13 69.3
MS.gene064108.t1 MTR_2g046830 27.126 247 158 10 28 261 110 347 4.68e-13 69.3
MS.gene064108.t1 MTR_2g066200 26.538 260 154 10 59 291 137 386 7.62e-13 68.6
MS.gene064108.t1 MTR_5g069390 24.742 291 179 12 34 293 136 417 8.55e-13 68.9
MS.gene064108.t1 MTR_4g014370 23.488 281 168 10 48 291 137 407 1.09e-12 68.2
MS.gene064108.t1 MTR_7g079640 26.860 242 149 8 59 284 136 365 1.13e-12 68.2
MS.gene064108.t1 MTR_7g407070 29.891 184 100 8 89 249 184 361 1.58e-12 67.8
MS.gene064108.t1 MTR_2g426060 28.028 289 151 15 39 289 95 364 1.63e-12 67.8
MS.gene064108.t1 MTR_2g078870 26.236 263 163 12 44 288 58 307 2.55e-12 66.6
MS.gene064108.t1 MTR_4g028680 25.490 306 184 12 25 291 98 398 2.59e-12 67.0
MS.gene064108.t1 MTR_2g046900 25.256 293 167 14 7 266 91 364 2.63e-12 67.0
MS.gene064108.t1 MTR_0831s0010 27.232 224 128 7 52 249 161 375 4.13e-12 66.6
MS.gene064108.t1 MTR_0054s0090 24.483 290 172 10 43 291 121 404 4.41e-12 66.2
MS.gene064108.t1 MTR_2g021155 28.333 240 139 13 43 261 130 357 4.82e-12 67.0
MS.gene064108.t1 MTR_7g077970 28.947 228 126 9 40 236 113 335 6.62e-12 65.9
MS.gene064108.t1 MTR_5g064700 22.909 275 168 7 59 293 98 368 7.05e-12 65.5
MS.gene064108.t1 MTR_2g046920 24.632 272 183 10 9 266 89 352 7.27e-12 65.5
MS.gene064108.t1 MTR_2g047878 27.757 263 164 10 41 288 112 363 7.35e-12 65.5
MS.gene064108.t1 MTR_7g017130 26.689 296 178 12 30 294 111 398 7.50e-12 65.9
MS.gene064108.t1 MTR_7g017170 28.400 250 142 11 30 250 111 352 9.04e-12 65.5
MS.gene064108.t1 MTR_4g007940 26.180 233 122 10 54 248 75 295 9.08e-12 65.1
MS.gene064108.t1 MTR_5g011200 27.861 201 119 10 43 228 117 306 9.51e-12 65.1
MS.gene064108.t1 MTR_2g021950 25.279 269 166 10 49 289 132 393 9.58e-12 65.5
MS.gene064108.t1 MTR_3g083640 27.273 264 155 15 44 288 157 402 1.11e-11 65.1
MS.gene064108.t1 MTR_7g070830 27.715 267 130 13 41 249 114 375 1.22e-11 65.1
MS.gene064108.t1 MTR_6g079810 23.667 300 175 10 43 294 171 464 1.36e-11 65.1
MS.gene064108.t1 MTR_3g036290 30.366 191 115 9 41 227 117 293 1.38e-11 64.7
MS.gene064108.t1 MTR_8g063370 24.468 188 124 4 51 222 134 319 1.57e-11 64.7
MS.gene064108.t1 MTR_5g096630 27.273 154 90 4 57 188 143 296 1.89e-11 64.7
MS.gene064108.t1 MTR_2g426020 26.210 248 152 9 43 263 100 343 2.11e-11 64.3
MS.gene064108.t1 MTR_2g047832 24.315 292 183 10 7 266 92 377 2.36e-11 64.3
MS.gene064108.t1 MTR_0054s0100 23.973 292 173 10 43 291 121 406 2.51e-11 64.3
MS.gene064108.t1 MTR_5g072060 28.364 275 168 12 4 258 71 336 2.63e-11 64.3
MS.gene064108.t1 MTR_7g061940 26.984 189 120 5 90 261 79 266 2.86e-11 63.2
MS.gene064108.t1 MTR_3g013740 25.551 227 141 9 43 249 149 367 3.15e-11 63.9
MS.gene064108.t1 MTR_2g031720 25.000 284 187 9 38 296 91 373 3.39e-11 63.5
MS.gene064108.t1 MTR_8g064890 24.490 245 153 9 15 234 94 331 3.68e-11 63.5
MS.gene064108.t1 MTR_5g096710 28.025 314 161 16 27 288 98 398 4.59e-11 63.2
MS.gene064108.t1 MTR_7g069550 26.148 283 155 15 43 285 123 391 5.67e-11 63.2
MS.gene064108.t1 MTR_2g024100 28.936 235 127 10 24 223 77 306 7.69e-11 62.8
MS.gene064108.t1 MTR_5g097680 23.723 274 167 7 59 294 159 428 8.06e-11 62.8
MS.gene064108.t1 MTR_3g010870 26.394 269 166 15 39 288 129 384 9.14e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064108.t1 AT3G06240 30.303 264 163 10 39 288 163 419 4.10e-17 81.3
MS.gene064108.t1 AT4G12560 26.502 283 173 12 38 287 88 368 1.28e-11 65.1
MS.gene064108.t1 AT4G12560 26.502 283 173 12 38 287 88 368 1.28e-11 65.1
MS.gene064108.t1 AT4G12560 26.502 283 173 12 38 287 88 368 1.28e-11 65.1
MS.gene064108.t1 AT4G22390 24.653 288 185 11 39 296 89 374 9.53e-11 62.4

Find 59 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAGTTGCGTTTGATTTAA+TGG 0.059074 4.3:-75347174 MS.gene064108:CDS
CCCATTCATATACATGGTTT+TGG 0.200644 4.3:-75347404 MS.gene064108:CDS
GGAAAAGGGTTTCTATTTAA+TGG 0.233671 4.3:-75347242 MS.gene064108:CDS
AATGATAAGGGACAGTTATT+AGG 0.237016 4.3:-75346873 MS.gene064108:CDS
GGGCCAACGTGCTGATAATT+TGG 0.244876 4.3:+75347424 None:intergenic
ATAATGATACAATTGTAATA+TGG 0.250687 4.3:-75347046 MS.gene064108:CDS
GAAAAGGGTTTCTATTTAAT+GGG 0.316810 4.3:-75347241 MS.gene064108:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.324275 4.3:-75347082 MS.gene064108:CDS
TTATGTGGTCGTTCCTTCTC+AGG 0.324627 4.3:-75347264 MS.gene064108:CDS
TATTTAATGGGGTTATTCAT+TGG 0.326421 4.3:-75347229 MS.gene064108:CDS
GTCGTTCCTTCTCAGGGAAA+AGG 0.351348 4.3:-75347257 MS.gene064108:CDS
TTCCCATGTTTCATCACATT+GGG 0.376756 4.3:-75347342 MS.gene064108:CDS
ATGATAAGGGACAGTTATTA+GGG 0.377615 4.3:-75346872 MS.gene064108:CDS
GGATTCCATATGTAGATGTT+TGG 0.388278 4.3:+75347491 None:intergenic
TCAACCGGAAAATAAGATTC+CGG 0.403939 4.3:+75347557 None:intergenic
TAATGATACAATTGTAATAT+GGG 0.406961 4.3:-75347045 MS.gene064108:CDS
TAATGGGGTTATTCATTGGC+TGG 0.411512 4.3:-75347225 MS.gene064108:CDS
CCCAAAACCATGTATATGAA+TGG 0.412703 4.3:+75347403 None:intergenic
TTATCGCCGTGATTTAGATT+TGG 0.416373 4.3:-75347201 MS.gene064108:CDS
GAGTCTCTGCTTTCACTGCT+TGG 0.417751 4.3:-75346804 MS.gene064108:CDS
CACGAACCTCTAATTTCAAC+CGG 0.420465 4.3:+75347542 None:intergenic
TTAGTGAAGTATAATGATAA+GGG 0.423081 4.3:-75346885 MS.gene064108:CDS
CGTTGGCCCATTCATATACA+TGG 0.423444 4.3:-75347410 MS.gene064108:CDS
ACTCTTGTGCTTCCTCTTCG+TGG 0.426296 4.3:-75346984 MS.gene064108:CDS
TGAGAAGGAACGACCACATA+AGG 0.430947 4.3:+75347266 None:intergenic
TTGTTAAAATCACTTGGAAC+AGG 0.434624 4.3:+75347131 None:intergenic
ATTAGTGAAGTATAATGATA+AGG 0.446149 4.3:-75346886 MS.gene064108:CDS
TGGTATTCACTTCCCTTATG+TGG 0.458462 4.3:-75347279 MS.gene064108:CDS
TTCAGATTGACTGTTACAAA+TGG 0.461693 4.3:+75346842 None:intergenic
AAATTAAGGTTCACTGAAGC+AGG 0.474047 4.3:+75347587 None:intergenic
GTCATCCAAACATCTACATA+TGG 0.477819 4.3:-75347496 MS.gene064108:CDS
CCATGTTTCATCACATTGGG+AGG 0.478150 4.3:-75347339 MS.gene064108:CDS
CCAGTCAAGAGATGATTACT+TGG 0.479956 4.3:-75347375 MS.gene064108:CDS
ATAGGTTGAAAGTAGTGATC+AGG 0.481665 4.3:+75346957 None:intergenic
GATATTATTGGAAGAAATCA+TGG 0.485431 4.3:-75346915 MS.gene064108:CDS
TTTCCCATGTTTCATCACAT+TGG 0.493098 4.3:-75347343 MS.gene064108:CDS
AATACAAAATGCATTCGTCT+TGG 0.493649 4.3:-75347013 MS.gene064108:CDS
CCAAAACCATGTATATGAAT+GGG 0.494804 4.3:+75347404 None:intergenic
TCGTTCCTTCTCAGGGAAAA+GGG 0.494994 4.3:-75347256 MS.gene064108:CDS
TTGGAACGAAAAGGAGAGAC+AGG 0.505714 4.3:+75347443 None:intergenic
CCTCCCAATGTGATGAAACA+TGG 0.513954 4.3:+75347339 None:intergenic
GCTGATAATTTGGAACGAAA+AGG 0.517095 4.3:+75347434 None:intergenic
AATATGTGTAAAGAAATTGA+TGG 0.520274 4.3:-75347299 MS.gene064108:CDS
GAAATTAGAGGTTCGTGTAG+AGG 0.526536 4.3:-75347536 MS.gene064108:CDS
AATCTATTGTTAAAATCACT+TGG 0.528379 4.3:+75347125 None:intergenic
AAATTAGAGGTTCGTGTAGA+GGG 0.528917 4.3:-75347535 MS.gene064108:CDS
TACATATGGAATCCATGCAC+TGG 0.535982 4.3:-75347482 MS.gene064108:CDS
TATGTGGTCGTTCCTTCTCA+GGG 0.544535 4.3:-75347263 MS.gene064108:CDS
GATCGAACTTCATGAGGTAG+AGG 0.559889 4.3:+75347641 None:intergenic
GAGAAGGAACGACCACATAA+GGG 0.562312 4.3:+75347267 None:intergenic
GTCAAGAGATGATTACTTGG+TGG 0.564802 4.3:-75347372 MS.gene064108:CDS
CCAAGTAATCATCTCTTGAC+TGG 0.573666 4.3:+75347375 None:intergenic
AAAAGGGTTTCTATTTAATG+GGG 0.578558 4.3:-75347240 MS.gene064108:CDS
CTCCCAATGTGATGAAACAT+GGG 0.599251 4.3:+75347340 None:intergenic
ATATGTAGATGTTTGGATGA+CGG 0.606207 4.3:+75347498 None:intergenic
TCTATAGATCGAACTTCATG+AGG 0.625514 4.3:+75347635 None:intergenic
TGACATCCAAATCTAAATCA+CGG 0.626162 4.3:+75347195 None:intergenic
GTTCCAAATTATCAGCACGT+TGG 0.650392 4.3:-75347427 MS.gene064108:CDS
TGATCAGGAATGCCACGAAG+AGG 0.662656 4.3:+75346972 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATGATACAATTGTAATA+TGG - chr4.3:75347392-75347411 MS.gene064108:CDS 15.0%
!! TAATGATACAATTGTAATAT+GGG - chr4.3:75347393-75347412 MS.gene064108:CDS 15.0%
!!! ATAGATTTCTTTGTAATTTT+TGG - chr4.3:75347329-75347348 MS.gene064108:CDS 15.0%
!!! TAGATTTCTTTGTAATTTTT+GGG - chr4.3:75347330-75347349 MS.gene064108:CDS 15.0%
!! AATATGTGTAAAGAAATTGA+TGG - chr4.3:75347139-75347158 MS.gene064108:CDS 20.0%
!! AATCTATTGTTAAAATCACT+TGG + chr4.3:75347316-75347335 None:intergenic 20.0%
!! ACAAAAAATGGTGATATTAT+TGG - chr4.3:75347511-75347530 MS.gene064108:CDS 20.0%
!! ATTAGTGAAGTATAATGATA+AGG - chr4.3:75347552-75347571 MS.gene064108:CDS 20.0%
!! CCTATATATTCTACAAAAAA+TGG - chr4.3:75347499-75347518 MS.gene064108:CDS 20.0%
!! TTAGTGAAGTATAATGATAA+GGG - chr4.3:75347553-75347572 MS.gene064108:CDS 20.0%
!!! CCATTTTTTGTAGAATATAT+AGG + chr4.3:75347502-75347521 None:intergenic 20.0%
! AAAAGGGTTTCTATTTAATG+GGG - chr4.3:75347198-75347217 MS.gene064108:CDS 25.0%
! GAAAAGGGTTTCTATTTAAT+GGG - chr4.3:75347197-75347216 MS.gene064108:CDS 25.0%
! GATATTATTGGAAGAAATCA+TGG - chr4.3:75347523-75347542 MS.gene064108:CDS 25.0%
! TATTTAATGGGGTTATTCAT+TGG - chr4.3:75347209-75347228 MS.gene064108:CDS 25.0%
!!! CTTTGTAATTTTTGGGTATT+TGG - chr4.3:75347337-75347356 MS.gene064108:CDS 25.0%
AATACAAAATGCATTCGTCT+TGG - chr4.3:75347425-75347444 MS.gene064108:CDS 30.0%
AATGATAAGGGACAGTTATT+AGG - chr4.3:75347565-75347584 MS.gene064108:CDS 30.0%
ATATGTAGATGTTTGGATGA+CGG + chr4.3:75346943-75346962 None:intergenic 30.0%
ATGATAAGGGACAGTTATTA+GGG - chr4.3:75347566-75347585 MS.gene064108:CDS 30.0%
CCAAAACCATGTATATGAAT+GGG + chr4.3:75347037-75347056 None:intergenic 30.0%
GGAAAAGGGTTTCTATTTAA+TGG - chr4.3:75347196-75347215 MS.gene064108:CDS 30.0%
TGACATCCAAATCTAAATCA+CGG + chr4.3:75347246-75347265 None:intergenic 30.0%
TTCAGATTGACTGTTACAAA+TGG + chr4.3:75347599-75347618 None:intergenic 30.0%
TTGTTAAAATCACTTGGAAC+AGG + chr4.3:75347310-75347329 None:intergenic 30.0%
! CATAGTTGCGTTTGATTTAA+TGG - chr4.3:75347264-75347283 MS.gene064108:CDS 30.0%
! CCATTCATATACATGGTTTT+GGG - chr4.3:75347035-75347054 MS.gene064108:CDS 30.0%
! TATTTTCCGGTTGAAATTAG+AGG - chr4.3:75346890-75346909 MS.gene064108:CDS 30.0%
AAATTAAGGTTCACTGAAGC+AGG + chr4.3:75346854-75346873 None:intergenic 35.0%
AAATTAGAGGTTCGTGTAGA+GGG - chr4.3:75346903-75346922 MS.gene064108:CDS 35.0%
ATTCCGGAAGCGAAAAATTA+AGG + chr4.3:75346868-75346887 None:intergenic 35.0%
CCCAAAACCATGTATATGAA+TGG + chr4.3:75347038-75347057 None:intergenic 35.0%
GGATTCCATATGTAGATGTT+TGG + chr4.3:75346950-75346969 None:intergenic 35.0%
GTCATCCAAACATCTACATA+TGG - chr4.3:75346942-75346961 MS.gene064108:CDS 35.0%
TCAACCGGAAAATAAGATTC+CGG + chr4.3:75346884-75346903 None:intergenic 35.0%
TCTATAGATCGAACTTCATG+AGG + chr4.3:75346806-75346825 None:intergenic 35.0%
TTATCGCCGTGATTTAGATT+TGG - chr4.3:75347237-75347256 MS.gene064108:CDS 35.0%
TTCCCATGTTTCATCACATT+GGG - chr4.3:75347096-75347115 MS.gene064108:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr4.3:75347356-75347375 MS.gene064108:CDS 35.0%
TTTCCCATGTTTCATCACAT+TGG - chr4.3:75347095-75347114 MS.gene064108:CDS 35.0%
! ATAGGTTGAAAGTAGTGATC+AGG + chr4.3:75347484-75347503 None:intergenic 35.0%
! CCCATTCATATACATGGTTT+TGG - chr4.3:75347034-75347053 MS.gene064108:CDS 35.0%
! GAACCTTAATTTTTCGCTTC+CGG - chr4.3:75346862-75346881 MS.gene064108:CDS 35.0%
! GCTGATAATTTGGAACGAAA+AGG + chr4.3:75347007-75347026 None:intergenic 35.0%
!! TGTGTTTTAAATCCAGTGCA+TGG + chr4.3:75346971-75346990 None:intergenic 35.0%
CACGAACCTCTAATTTCAAC+CGG + chr4.3:75346899-75346918 None:intergenic 40.0%
CCAAGTAATCATCTCTTGAC+TGG + chr4.3:75347066-75347085 None:intergenic 40.0%
CCAGTCAAGAGATGATTACT+TGG - chr4.3:75347063-75347082 MS.gene064108:CDS 40.0%
CTCCCAATGTGATGAAACAT+GGG + chr4.3:75347101-75347120 None:intergenic 40.0%
GAAATTAGAGGTTCGTGTAG+AGG - chr4.3:75346902-75346921 MS.gene064108:CDS 40.0%
GTCAAGAGATGATTACTTGG+TGG - chr4.3:75347066-75347085 MS.gene064108:CDS 40.0%
GTTCCAAATTATCAGCACGT+TGG - chr4.3:75347011-75347030 MS.gene064108:CDS 40.0%
TAATGGGGTTATTCATTGGC+TGG - chr4.3:75347213-75347232 MS.gene064108:CDS 40.0%
TACATATGGAATCCATGCAC+TGG - chr4.3:75346956-75346975 MS.gene064108:CDS 40.0%
TGGTATTCACTTCCCTTATG+TGG - chr4.3:75347159-75347178 MS.gene064108:CDS 40.0%
! ATCACATTGGGAGGTTTTCT+CGG - chr4.3:75347108-75347127 MS.gene064108:CDS 40.0%
!!! GCTTCCGGAATCTTATTTTC+CGG - chr4.3:75346877-75346896 MS.gene064108:CDS 40.0%
CCATGTTTCATCACATTGGG+AGG - chr4.3:75347099-75347118 MS.gene064108:CDS 45.0%
CCTCCCAATGTGATGAAACA+TGG + chr4.3:75347102-75347121 None:intergenic 45.0%
CGTTGGCCCATTCATATACA+TGG - chr4.3:75347028-75347047 MS.gene064108:CDS 45.0%
GAGAAGGAACGACCACATAA+GGG + chr4.3:75347174-75347193 None:intergenic 45.0%
GATCGAACTTCATGAGGTAG+AGG + chr4.3:75346800-75346819 None:intergenic 45.0%
TATGTGGTCGTTCCTTCTCA+GGG - chr4.3:75347175-75347194 MS.gene064108:CDS 45.0%
TCGTTCCTTCTCAGGGAAAA+GGG - chr4.3:75347182-75347201 MS.gene064108:CDS 45.0%
TGAGAAGGAACGACCACATA+AGG + chr4.3:75347175-75347194 None:intergenic 45.0%
TTATGTGGTCGTTCCTTCTC+AGG - chr4.3:75347174-75347193 MS.gene064108:CDS 45.0%
TTGGAACGAAAAGGAGAGAC+AGG + chr4.3:75346998-75347017 None:intergenic 45.0%
! AGAAACCCTTTTCCCTGAGA+AGG + chr4.3:75347190-75347209 None:intergenic 45.0%
ACTCTTGTGCTTCCTCTTCG+TGG - chr4.3:75347454-75347473 MS.gene064108:CDS 50.0%
GAGTCTCTGCTTTCACTGCT+TGG - chr4.3:75347634-75347653 MS.gene064108:CDS 50.0%
GTCGTTCCTTCTCAGGGAAA+AGG - chr4.3:75347181-75347200 MS.gene064108:CDS 50.0%
TGATCAGGAATGCCACGAAG+AGG + chr4.3:75347469-75347488 None:intergenic 50.0%
! GGGCCAACGTGCTGATAATT+TGG + chr4.3:75347017-75347036 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 75346785 75347675 75346785 ID=MS.gene064108
chr4.3 mRNA 75346785 75347675 75346785 ID=MS.gene064108.t1;Parent=MS.gene064108
chr4.3 exon 75346785 75347675 75346785 ID=MS.gene064108.t1.exon1;Parent=MS.gene064108.t1
chr4.3 CDS 75346785 75347675 75346785 ID=cds.MS.gene064108.t1;Parent=MS.gene064108.t1
Gene Sequence

>MS.gene064108

ATGTGCATATCACCTCTACCTCATGAAGTTCGATCTATAGATTTTAAAGCATTTCTTAATGATGATCCTGCTTCAGTGAACCTTAATTTTTCGCTTCCGGAATCTTATTTTCCGGTTGAAATTAGAGGTTCGTGTAGAGGGTTCATACTTTTGTACCGTCATCCAAACATCTACATATGGAATCCATGCACTGGATTTAAAACACAAATACCTGTCTCTCCTTTTCGTTCCAAATTATCAGCACGTTGGCCCATTCATATACATGGTTTTGGGTATGACCAGTCAAGAGATGATTACTTGGTGGTTGTATTTTCCCATGTTTCATCACATTGGGAGGTTTTCTCGGTTAGAGATAATATGTGTAAAGAAATTGATGGTATTCACTTCCCTTATGTGGTCGTTCCTTCTCAGGGAAAAGGGTTTCTATTTAATGGGGTTATTCATTGGCTGGCTTATCGCCGTGATTTAGATTTGGATGTCATAGTTGCGTTTGATTTAATGGAAATGAAAATGTTTGAGATGCCTGTTCCAAGTGATTTTAACAATAGATTTCTTTGTAATTTTTGGGTATTTGGAGAATTTCTCAGTCTATGGTATAAGAATAATTATAATGATACAATTGTAATATGGGTGATGAAAGAATACAAAATGCATTCGTCTTGGACTAAAACTCTTGTGCTTCCTCTTCGTGGCATTCCTGATCACTACTTTCAACCTATATATTCTACAAAAAATGGTGATATTATTGGAAGAAATCATGGTACTAGATTAGTGAAGTATAATGATAAGGGACAGTTATTAGGGCAGCGCTCCATTTGTAACAGTCAATCTGAAGTTGTCATGTATACAGAGTCTCTGCTTTCACTGCTTGGTGACAATGAGCATGCTTAA
Protein sequence

>MS.gene064108.t1

MCISPLPHEVRSIDFKAFLNDDPASVNLNFSLPESYFPVEIRGSCRGFILLYRHPNIYIWNPCTGFKTQIPVSPFRSKLSARWPIHIHGFGYDQSRDDYLVVVFSHVSSHWEVFSVRDNMCKEIDGIHFPYVVVPSQGKGFLFNGVIHWLAYRRDLDLDVIVAFDLMEMKMFEMPVPSDFNNRFLCNFWVFGEFLSLWYKNNYNDTIVIWVMKEYKMHSSWTKTLVLPLRGIPDHYFQPIYSTKNGDIIGRNHGTRLVKYNDKGQLLGQRSICNSQSEVVMYTESLLSLLGDNEHA