AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44886

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44886.t1 MTR_5g070088 92.049 327 26 0 1 327 60 386 0.0 587
MS.gene44886.t1 MTR_5g070080 67.683 328 101 4 1 327 63 386 3.35e-140 402
MS.gene44886.t1 MTR_5g070130 59.710 345 112 4 1 338 64 388 1.50e-136 393
MS.gene44886.t1 MTR_3g103020 52.012 323 132 4 1 321 72 373 1.46e-97 294
MS.gene44886.t1 MTR_4g117720 49.699 332 132 9 4 326 72 377 6.16e-92 279
MS.gene44886.t1 MTR_5g097210 47.941 340 144 9 1 337 64 373 3.81e-90 275
MS.gene44886.t1 MTR_3g034160 48.036 331 136 10 7 326 69 374 1.98e-79 247
MS.gene44886.t1 MTR_3g034180 46.269 335 144 10 4 327 180 489 2.53e-79 250
MS.gene44886.t1 MTR_4g023080 45.079 315 131 7 11 321 73 349 2.96e-77 241
MS.gene44886.t1 MTR_1g055195 45.345 333 140 10 15 330 174 481 1.29e-76 243
MS.gene44886.t1 MTR_1g055135 49.822 281 121 8 52 321 99 370 1.79e-76 239
MS.gene44886.t1 MTR_4g117750 46.988 332 129 9 1 320 57 353 1.24e-75 241
MS.gene44886.t1 MTR_4g117750 43.846 130 64 2 200 321 350 478 1.94e-19 89.4
MS.gene44886.t1 MTR_4g109270 45.171 321 132 8 11 321 73 359 2.33e-74 234
MS.gene44886.t1 MTR_6g060650 44.479 326 148 7 5 320 65 367 1.27e-73 232
MS.gene44886.t1 MTR_4g023090 43.574 319 142 9 30 327 85 386 1.21e-72 230
MS.gene44886.t1 MTR_4g122850 43.658 339 157 11 7 337 62 374 5.78e-72 228
MS.gene44886.t1 MTR_4g094365 46.644 298 140 8 33 320 70 358 6.13e-72 228
MS.gene44886.t1 MTR_3g034130 43.750 320 146 8 4 312 66 362 2.85e-71 226
MS.gene44886.t1 MTR_4g023130 48.872 266 114 6 60 321 61 308 3.87e-71 224
MS.gene44886.t1 MTR_4g109280 47.040 321 141 8 1 319 59 352 2.13e-70 223
MS.gene44886.t1 MTR_4g117850 46.497 314 135 8 11 319 88 373 5.02e-70 223
MS.gene44886.t1 MTR_4g023070 41.265 332 156 7 11 329 67 372 5.48e-67 215
MS.gene44886.t1 MTR_4g023120 41.718 326 152 7 11 323 67 367 5.62e-67 215
MS.gene44886.t1 MTR_8g433040 42.638 326 149 10 5 327 74 364 1.01e-66 214
MS.gene44886.t1 MTR_8g032380 42.638 326 149 10 5 327 74 364 1.01e-66 214
MS.gene44886.t1 MTR_4g023190 42.453 318 139 10 11 321 74 354 1.16e-66 214
MS.gene44886.t1 MTR_4g118380 43.322 307 148 7 5 299 65 357 2.21e-66 213
MS.gene44886.t1 MTR_4g021785 42.089 316 140 8 11 321 73 350 1.14e-65 211
MS.gene44886.t1 MTR_7g079400 42.500 320 143 10 15 322 43 333 1.78e-65 210
MS.gene44886.t1 MTR_4g023150 41.905 315 146 9 11 321 74 355 3.59e-65 210
MS.gene44886.t1 MTR_4g117870 40.432 324 134 8 4 327 3 267 1.49e-64 205
MS.gene44886.t1 MTR_4g023200 41.066 319 143 10 11 321 74 355 2.56e-64 208
MS.gene44886.t1 MTR_4g117780 43.478 322 151 8 11 327 72 367 2.82e-64 208
MS.gene44886.t1 MTR_4g117770 43.963 323 148 9 11 327 72 367 3.34e-64 207
MS.gene44886.t1 MTR_4g117810 43.963 323 148 9 11 327 72 367 3.34e-64 207
MS.gene44886.t1 MTR_4g023380 39.496 357 140 11 1 327 69 379 5.91e-64 207
MS.gene44886.t1 MTR_4g118370 50.000 246 95 6 78 320 34 254 1.09e-63 203
MS.gene44886.t1 MTR_8g032390 48.302 265 126 7 63 322 113 371 1.49e-63 206
MS.gene44886.t1 MTR_4g116330 47.510 261 128 5 63 321 122 375 3.29e-63 206
MS.gene44886.t1 MTR_7g079370 41.212 330 154 9 8 322 48 352 2.70e-61 200
MS.gene44886.t1 MTR_4g023440 41.824 318 116 12 11 327 83 332 9.65e-60 195
MS.gene44886.t1 MTR_8g102520 46.215 251 122 8 78 322 75 318 1.72e-55 184
MS.gene44886.t1 MTR_4g118400 39.672 305 144 9 23 320 23 294 2.06e-55 183
MS.gene44886.t1 MTR_1g055250 46.888 241 108 10 104 337 49 276 7.73e-54 180
MS.gene44886.t1 MTR_8g433030 49.545 220 103 6 105 322 125 338 1.73e-51 174
MS.gene44886.t1 MTR_8g013010 46.053 228 94 6 1 225 108 309 2.11e-51 173
MS.gene44886.t1 MTR_4g116320 50.000 214 101 4 109 320 43 252 3.11e-51 171
MS.gene44886.t1 MTR_4g121580 39.550 311 145 10 17 319 67 342 9.10e-51 173
MS.gene44886.t1 MTR_1g055165 39.852 271 108 6 52 321 97 313 3.78e-49 167
MS.gene44886.t1 MTR_1g055175 48.168 191 92 5 134 320 8 195 2.15e-48 162
MS.gene44886.t1 MTR_4g116330 42.529 261 102 7 63 321 122 336 2.56e-47 163
MS.gene44886.t1 MTR_6g463430 45.545 202 105 1 120 321 1 197 1.79e-46 157
MS.gene44886.t1 MTR_2g048580 38.158 304 145 14 26 322 71 338 2.37e-43 153
MS.gene44886.t1 MTR_8g040935 44.444 207 106 6 120 321 1 203 2.47e-41 144
MS.gene44886.t1 MTR_7g079420 39.130 276 111 11 64 322 905 1140 8.47e-41 152
MS.gene44886.t1 MTR_4g117530 37.549 253 110 6 74 321 39 248 1.14e-37 135
MS.gene44886.t1 MTR_4g117430 45.399 163 74 5 65 225 3 152 2.70e-37 131
MS.gene44886.t1 MTR_4g114700 37.931 261 103 7 65 317 9 218 4.81e-37 133
MS.gene44886.t1 MTR_7g079450 49.286 140 63 4 184 322 1 133 7.61e-34 122
MS.gene44886.t1 MTR_7g117720 46.622 148 54 5 11 153 55 182 3.55e-29 113
MS.gene44886.t1 MTR_7g117720 51.000 100 44 3 225 320 173 271 1.49e-19 87.4
MS.gene44886.t1 MTR_8g098830 38.947 190 103 6 135 320 35 215 3.30e-28 110
MS.gene44886.t1 MTR_4g116340 45.185 135 65 3 62 190 116 247 7.92e-27 106
MS.gene44886.t1 MTR_4g118360 49.194 124 56 4 200 320 1 120 1.36e-26 102
MS.gene44886.t1 MTR_5g023000 56.818 88 38 0 234 321 7 94 3.77e-24 95.1
MS.gene44886.t1 MTR_4g122860 33.498 203 103 5 140 338 157 331 2.51e-22 96.3
MS.gene44886.t1 MTR_4g133170 29.832 238 141 11 57 277 85 313 7.47e-20 90.1
MS.gene44886.t1 MTR_1g055200 50.602 83 39 1 61 143 5 85 3.56e-19 82.0
MS.gene44886.t1 MTR_6g034720 34.973 183 105 4 29 207 1 173 4.15e-19 85.1
MS.gene44886.t1 MTR_2g026010 30.126 239 147 10 57 277 85 321 4.94e-19 87.8
MS.gene44886.t1 MTR_5g069120 38.514 148 61 4 6 143 50 177 6.96e-19 83.2
MS.gene44886.t1 MTR_3g083010 28.205 312 181 13 62 337 108 412 1.52e-18 86.3
MS.gene44886.t1 MTR_2g028130 29.719 249 137 12 57 277 101 339 3.96e-18 85.1
MS.gene44886.t1 MTR_2g024110 26.423 246 162 7 62 294 93 332 1.02e-17 83.6
MS.gene44886.t1 MTR_4g023430 51.807 83 36 2 248 327 141 222 1.03e-17 81.3
MS.gene44886.t1 MTR_2g028140 28.889 270 151 13 20 258 68 327 1.05e-17 84.0
MS.gene44886.t1 MTR_2g028160 30.833 240 138 11 62 277 106 341 1.07e-16 80.9
MS.gene44886.t1 MTR_2g025960 32.420 219 115 12 62 255 5 215 1.65e-16 79.3
MS.gene44886.t1 MTR_4g070550 28.800 250 142 10 58 277 97 340 4.89e-16 79.0
MS.gene44886.t1 MTR_1g068875 27.074 229 149 7 62 277 91 314 6.53e-16 78.2
MS.gene44886.t1 MTR_2g031640 28.692 237 149 8 57 277 87 319 7.17e-16 78.2
MS.gene44886.t1 MTR_2g024100 29.864 221 124 9 57 251 91 306 9.13e-16 78.2
MS.gene44886.t1 MTR_1g097490 27.715 267 169 11 59 318 141 390 1.08e-15 77.8
MS.gene44886.t1 MTR_1g068835 27.876 226 138 8 48 256 75 292 1.13e-15 77.8
MS.gene44886.t1 MTR_0667s0010 27.755 245 140 11 62 277 94 330 3.71e-15 76.3
MS.gene44886.t1 MTR_2g426070 28.452 239 142 7 62 277 105 337 4.16e-15 76.3
MS.gene44886.t1 MTR_2g021050 28.279 244 127 11 58 277 91 310 4.68e-15 75.9
MS.gene44886.t1 MTR_6g090180 25.091 275 168 8 74 319 169 434 6.19e-15 75.9
MS.gene44886.t1 MTR_4g028510 27.341 267 166 8 78 321 139 400 9.30e-15 75.5
MS.gene44886.t1 MTR_2g025420 27.915 283 161 12 33 277 69 346 3.25e-14 73.6
MS.gene44886.t1 MTR_7g078860 28.365 208 129 4 62 257 122 321 5.95e-14 72.8
MS.gene44886.t1 MTR_7g056760 23.724 333 206 10 20 318 86 404 1.33e-13 71.6
MS.gene44886.t1 MTR_2g426090 28.216 241 142 10 62 277 100 334 1.43e-13 71.6
MS.gene44886.t1 MTR_2g426060 29.279 222 121 12 62 255 99 312 1.98e-13 71.2
MS.gene44886.t1 MTR_3g030250 27.556 225 129 10 40 255 111 310 2.31e-13 70.9
MS.gene44886.t1 MTR_1g068860 26.291 213 140 7 57 256 86 294 3.39e-13 70.1
MS.gene44886.t1 MTR_2g031720 25.685 292 196 11 62 337 96 382 4.73e-13 70.1
MS.gene44886.t1 MTR_4g013175 27.660 235 113 8 62 257 123 339 4.80e-13 70.1
MS.gene44886.t1 MTR_7g056260 27.698 278 133 10 13 257 75 317 9.38e-13 68.6
MS.gene44886.t1 MTR_7g056390 28.959 221 113 9 62 257 132 333 9.47e-13 68.9
MS.gene44886.t1 MTR_2g021150 26.407 231 152 9 39 261 90 310 1.33e-12 68.6
MS.gene44886.t1 MTR_2g021190 27.500 240 152 10 62 291 118 345 1.36e-12 68.6
MS.gene44886.t1 MTR_7g077910 29.224 219 119 7 62 257 44 249 1.85e-12 67.8
MS.gene44886.t1 MTR_2g426020 24.629 337 216 14 33 341 68 394 2.02e-12 68.2
MS.gene44886.t1 MTR_3g007430 26.119 268 157 12 8 255 82 328 2.42e-12 67.8
MS.gene44886.t1 MTR_4g013185 26.349 315 164 14 62 319 123 426 2.72e-12 67.8
MS.gene44886.t1 MTR_0054s0100 22.630 327 213 10 28 321 87 406 3.56e-12 67.4
MS.gene44886.t1 MTR_3g009310 27.723 202 129 6 60 256 138 327 4.62e-12 67.0
MS.gene44886.t1 MTR_3g072470 28.571 224 110 8 62 257 132 333 6.44e-12 66.6
MS.gene44886.t1 MTR_4g023100 56.140 57 22 2 271 327 66 119 8.06e-12 62.0
MS.gene44886.t1 MTR_2g021155 26.909 275 163 10 39 289 97 357 8.44e-12 66.6
MS.gene44886.t1 MTR_2g078870 26.904 197 120 8 60 251 55 232 1.27e-11 65.1
MS.gene44886.t1 MTR_8g467140 28.000 200 128 6 62 255 128 317 1.33e-11 65.1
MS.gene44886.t1 MTR_7g070440 28.516 256 144 11 30 259 67 309 1.35e-11 65.5
MS.gene44886.t1 MTR_4g109290 59.574 47 19 0 74 120 89 135 1.59e-11 62.8
MS.gene44886.t1 MTR_1g055145 45.977 87 43 3 153 239 117 199 2.38e-11 63.9
MS.gene44886.t1 MTR_5g011200 26.291 213 134 9 62 265 117 315 2.60e-11 64.7
MS.gene44886.t1 MTR_5g097680 26.148 283 164 10 73 321 154 425 2.89e-11 64.7
MS.gene44886.t1 MTR_2g055970 26.794 209 131 8 56 255 301 496 3.43e-11 64.7
MS.gene44886.t1 MTR_6g048120 27.103 214 134 7 48 251 125 326 3.73e-11 64.3
MS.gene44886.t1 MTR_2g043400 30.275 218 113 10 62 258 135 334 4.22e-11 63.9
MS.gene44886.t1 MTR_8g041270 24.566 346 200 12 21 321 75 404 5.83e-11 63.5
MS.gene44886.t1 MTR_4g034860 25.475 263 140 9 26 257 102 339 6.02e-11 63.5
MS.gene44886.t1 MTR_8g013005 56.863 51 22 0 271 321 4 54 7.26e-11 57.4
MS.gene44886.t1 MTR_2g047200 26.042 288 167 12 62 318 121 393 7.75e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44886.t1 AT4G12560 31.707 246 135 10 33 256 70 304 7.77e-18 84.3
MS.gene44886.t1 AT4G12560 31.707 246 135 10 33 256 70 304 7.77e-18 84.3
MS.gene44886.t1 AT4G12560 31.707 246 135 10 33 256 70 304 7.77e-18 84.3

Find 66 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAATTATTTCTATGGTTT+TGG 0.146874 5.2:+59328953 MS.gene44886:CDS
ATAGAGTTACATTTGAAATA+TGG 0.209026 5.2:+59329347 MS.gene44886:CDS
CTTCAAAACCTCATTCTTCT+TGG 0.218400 5.2:-59329653 None:intergenic
CCAGATGCAAATTATTTCTA+TGG 0.244716 5.2:+59328947 MS.gene44886:CDS
GCAATCCTGCTCACTCCTTT+TGG 0.280755 5.2:+59329431 MS.gene44886:CDS
CTAGCAGGGCGTATGATTTA+TGG 0.282269 5.2:+59329284 MS.gene44886:CDS
TACATTTGAAATATGGGTTA+TGG 0.283926 5.2:+59329354 MS.gene44886:CDS
AAAAGACTGTTCTGCGAATA+AGG 0.287100 5.2:-59329118 None:intergenic
GTTATTCAAGCCTCCGTATA+TGG 0.289005 5.2:+59328870 MS.gene44886:CDS
GAATCTCTGCTTTCAATTCC+TGG 0.293988 5.2:+59329595 MS.gene44886:CDS
TTGGAATCAATTGTAGTAAA+AGG 0.296434 5.2:-59328923 None:intergenic
CTGTCCTTTGTCATTATACT+TGG 0.343254 5.2:-59329512 None:intergenic
GGACCATTGATTATATCATT+TGG 0.357863 5.2:-59329262 None:intergenic
CGTTTGTGAAATCCGGTAGA+TGG 0.360277 5.2:-59328896 None:intergenic
TAGAGTTACATTTGAAATAT+GGG 0.361693 5.2:+59329348 MS.gene44886:CDS
AAATGAAAGGCAACAATAAC+AGG 0.367546 5.2:-59329208 None:intergenic
ATAGAGGATGTCTCGCTCTA+TGG 0.367959 5.2:+59329311 MS.gene44886:CDS
CGGTAGATGGATTCCATATA+CGG 0.378320 5.2:-59328883 None:intergenic
CCATAGAAATAATTTGCATC+TGG 0.394587 5.2:-59328947 None:intergenic
ATAGATAGAGCTTTGAGATA+TGG 0.401282 5.2:-59328668 None:intergenic
GAAATAATTTGCATCTGGAT+TGG 0.426106 5.2:-59328942 None:intergenic
TTAGTAGAGTCGATCTCAAA+TGG 0.430961 5.2:+59329145 MS.gene44886:CDS
AGGTATTCGTTTGTGAAATC+CGG 0.432435 5.2:-59328903 None:intergenic
TAGCAGGGCGTATGATTTAT+GGG 0.440191 5.2:+59329285 MS.gene44886:CDS
TTGCCTTTCATTTAATTGAA+AGG 0.455366 5.2:+59329218 MS.gene44886:CDS
TAGTAGAGTCGATCTCAAAT+GGG 0.462940 5.2:+59329146 MS.gene44886:CDS
TCTCAAATGGGGCTATTCAC+TGG 0.466499 5.2:+59329158 MS.gene44886:CDS
TATGATTTATGGGTGTATAG+AGG 0.477400 5.2:+59329295 MS.gene44886:CDS
CGAGTCAACAGATGATTACT+TGG 0.478220 5.2:+59328982 MS.gene44886:CDS
TTTCCTTTCAATTAAATGAA+AGG 0.478785 5.2:-59329221 None:intergenic
ATATAATCAATGGTCCTAGC+AGG 0.484409 5.2:+59329269 MS.gene44886:CDS
AATACAATGTGCAATCGTCT+TGG 0.487961 5.2:+59329380 MS.gene44886:CDS
TTGCCAAATGATATAATCAA+TGG 0.496144 5.2:+59329259 MS.gene44886:CDS
AAATGGGGCTATTCACTGGT+TGG 0.496742 5.2:+59329162 MS.gene44886:CDS
AGATGGTATGCTATCTCACT+TGG 0.498942 5.2:+59329039 MS.gene44886:CDS
TTTGGCCAAAAGGAGTGAGC+AGG 0.511619 5.2:-59329436 None:intergenic
TGAGCTCGGAGTCTGTTCAT+AGG 0.512785 5.2:-59329017 None:intergenic
TAGAGGATGTCTCGCTCTAT+GGG 0.527354 5.2:+59329312 MS.gene44886:CDS
TAATGTGTGGAGCGCAATTG+AGG 0.536865 5.2:+59329081 MS.gene44886:CDS
TGTTCTGCGAATAAGGCAAA+AGG 0.540223 5.2:-59329111 None:intergenic
TCAATTCCTGGTGGTGACAG+TGG 0.541479 5.2:+59329607 MS.gene44886:CDS
AAGAAACATGCGTGGTGCAT+TGG 0.549642 5.2:+59329489 MS.gene44886:CDS
GAACAGACTCCGAGCTCAGA+TGG 0.552016 5.2:+59329022 MS.gene44886:CDS
GAATGTGTATTACTCTGAGT+TGG 0.554743 5.2:-59330033 None:intergenic
CAGGATTGCCATCAAAAGAC+AGG 0.556670 5.2:-59329417 None:intergenic
CATAAATCATACGCCCTGCT+AGG 0.560538 5.2:-59329283 None:intergenic
CGTATATGGAATCCATCTAC+CGG 0.563507 5.2:+59328884 MS.gene44886:CDS
TGATAGTGCTTCATATCCAA+TGG 0.566505 5.2:+59328715 MS.gene44886:CDS
TAGTTCTTCTTCATCTGACA+TGG 0.567167 5.2:+59328772 MS.gene44886:CDS
TCTCATTGAGAGCTAATGTG+TGG 0.589751 5.2:+59329068 MS.gene44886:CDS
ACTTGACCACTGTCACCACC+AGG 0.592774 5.2:-59329613 None:intergenic
AATGTGTGGAGCGCAATTGA+GGG 0.603196 5.2:+59329082 MS.gene44886:CDS
TGACAGAACCAAGAAGAATG+AGG 0.608372 5.2:+59329645 MS.gene44886:CDS
TACATATGCATTGTCACAGT+AGG 0.609801 5.2:-59329551 None:intergenic
ATTCTGTATACATAACCACT+AGG 0.630116 5.2:-59329576 None:intergenic
GATGGTATGCTATCTCACTT+GGG 0.633795 5.2:+59329040 MS.gene44886:CDS
GATAGCATACCATCTGAGCT+CGG 0.644743 5.2:-59329031 None:intergenic
TATAATCAATGGTCCTAGCA+GGG 0.644894 5.2:+59329270 MS.gene44886:CDS
TTCTGTATACATAACCACTA+GGG 0.647948 5.2:-59329575 None:intergenic
CAATGCATATGTATCCCTAG+TGG 0.670534 5.2:+59329561 MS.gene44886:CDS
TTGGCCAAGTATAATGACAA+AGG 0.670777 5.2:+59329508 MS.gene44886:CDS
AGTAGAGTCGATCTCAAATG+GGG 0.672893 5.2:+59329147 MS.gene44886:CDS
TCTCTGCTTTCAATTCCTGG+TGG 0.696103 5.2:+59329598 MS.gene44886:CDS
TAGATGGATTCCATATACGG+AGG 0.723482 5.2:-59328880 None:intergenic
GTGTGGAGCGCAATTGAGGG+TGG 0.724694 5.2:+59329085 MS.gene44886:CDS
ATCGTTGGAAGAAACATGCG+TGG 0.741909 5.2:+59329481 MS.gene44886:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAAACTTGATTTTAA+AGG + chr5.2:59328818-59328837 MS.gene44886:CDS 10.0%
!! AATTATAAAGATCATGAAAA+TGG - chr5.2:59329691-59329710 None:intergenic 15.0%
!! ATCAAGTTTAAAAAAAGATT+TGG - chr5.2:59328813-59328832 None:intergenic 15.0%
!! TCAAGTTTAAAAAAAGATTT+GGG - chr5.2:59328812-59328831 None:intergenic 15.0%
!! TTTCCTTTCAATTAAATGAA+AGG - chr5.2:59329224-59329243 None:intergenic 20.0%
!!! ATAGAGTTACATTTGAAATA+TGG + chr5.2:59329347-59329366 MS.gene44886:CDS 20.0%
!!! CAAATTATTTCTATGGTTTT+GGG + chr5.2:59328954-59328973 MS.gene44886:CDS 20.0%
!!! TAGAGTTACATTTGAAATAT+GGG + chr5.2:59329348-59329367 MS.gene44886:CDS 20.0%
!!! TGTATTTTAATGTATGTTTG+TGG + chr5.2:59329914-59329933 MS.gene44886:intron 20.0%
! AAATGTCATGAAATGTTTAC+TGG + chr5.2:59329810-59329829 MS.gene44886:intron 25.0%
! AGTTTAAAAAAAGATTTGGG+AGG - chr5.2:59328809-59328828 None:intergenic 25.0%
! ATCAAAAAACTAATGTCCAT+TGG - chr5.2:59328734-59328753 None:intergenic 25.0%
! ATTTGTAATATGATCCAGTA+TGG + chr5.2:59329769-59329788 MS.gene44886:intron 25.0%
! CCAAAATACTATACAAAAAG+TGG + chr5.2:59329454-59329473 MS.gene44886:CDS 25.0%
! TTGCCAAATGATATAATCAA+TGG + chr5.2:59329259-59329278 MS.gene44886:CDS 25.0%
! TTGCCTTTCATTTAATTGAA+AGG + chr5.2:59329218-59329237 MS.gene44886:CDS 25.0%
! TTGGAATCAATTGTAGTAAA+AGG - chr5.2:59328926-59328945 None:intergenic 25.0%
!! TACATTTGAAATATGGGTTA+TGG + chr5.2:59329354-59329373 MS.gene44886:CDS 25.0%
!!! CCACTTTTTGTATAGTATTT+TGG - chr5.2:59329457-59329476 None:intergenic 25.0%
!!! GCAAATTATTTCTATGGTTT+TGG + chr5.2:59328953-59328972 MS.gene44886:CDS 25.0%
AAATGAAAGGCAACAATAAC+AGG - chr5.2:59329211-59329230 None:intergenic 30.0%
ATTCTGTATACATAACCACT+AGG - chr5.2:59329579-59329598 None:intergenic 30.0%
TAATGTATGTTTGTGGTCAT+TGG + chr5.2:59329921-59329940 MS.gene44886:intron 30.0%
TATGATTTATGGGTGTATAG+AGG + chr5.2:59329295-59329314 MS.gene44886:CDS 30.0%
TTCTGTATACATAACCACTA+GGG - chr5.2:59329578-59329597 None:intergenic 30.0%
TTGTATTATGTACTCTTGAG+TGG + chr5.2:59329714-59329733 MS.gene44886:intron 30.0%
! ATGTTATTTGTGCATGTGTA+TGG + chr5.2:59329889-59329908 MS.gene44886:intron 30.0%
! CCATAGAAATAATTTGCATC+TGG - chr5.2:59328950-59328969 None:intergenic 30.0%
! GAAATAATTTGCATCTGGAT+TGG - chr5.2:59328945-59328964 None:intergenic 30.0%
! GGACCATTGATTATATCATT+TGG - chr5.2:59329265-59329284 None:intergenic 30.0%
!! ATAGATAGAGCTTTGAGATA+TGG - chr5.2:59328671-59328690 None:intergenic 30.0%
!! CCAGATGCAAATTATTTCTA+TGG + chr5.2:59328947-59328966 MS.gene44886:CDS 30.0%
!!! TGTATAGTATTTTGGCCAAA+AGG - chr5.2:59329449-59329468 None:intergenic 30.0%
AAAAGACTGTTCTGCGAATA+AGG - chr5.2:59329121-59329140 None:intergenic 35.0%
AATACAATGTGCAATCGTCT+TGG + chr5.2:59329380-59329399 MS.gene44886:CDS 35.0%
ACAAAAAGTGGTGATATCGT+TGG + chr5.2:59329466-59329485 MS.gene44886:CDS 35.0%
AGGTATTCGTTTGTGAAATC+CGG - chr5.2:59328906-59328925 None:intergenic 35.0%
ATATAATCAATGGTCCTAGC+AGG + chr5.2:59329269-59329288 MS.gene44886:CDS 35.0%
CTGTCCTTTGTCATTATACT+TGG - chr5.2:59329515-59329534 None:intergenic 35.0%
CTTCAAAACCTCATTCTTCT+TGG - chr5.2:59329656-59329675 None:intergenic 35.0%
TACATATGCATTGTCACAGT+AGG - chr5.2:59329554-59329573 None:intergenic 35.0%
TAGTAGAGTCGATCTCAAAT+GGG + chr5.2:59329146-59329165 MS.gene44886:CDS 35.0%
TAGTTCTTCTTCATCTGACA+TGG + chr5.2:59328772-59328791 MS.gene44886:CDS 35.0%
TATAATCAATGGTCCTAGCA+GGG + chr5.2:59329270-59329289 MS.gene44886:CDS 35.0%
TTAGTAGAGTCGATCTCAAA+TGG + chr5.2:59329145-59329164 MS.gene44886:CDS 35.0%
TTGGCCAAGTATAATGACAA+AGG + chr5.2:59329508-59329527 MS.gene44886:CDS 35.0%
! TGATAGTGCTTCATATCCAA+TGG + chr5.2:59328715-59328734 MS.gene44886:CDS 35.0%
! TTGTGGTCATTGGTTGAAAT+TGG + chr5.2:59329931-59329950 MS.gene44886:intron 35.0%
!! GATTTTAAAGGTTCATGCAG+AGG + chr5.2:59328830-59328849 MS.gene44886:CDS 35.0%
!! TGTTGACTGCTTTGTTTCTA+TGG + chr5.2:59329862-59329881 MS.gene44886:intron 35.0%
AGATGGTATGCTATCTCACT+TGG + chr5.2:59329039-59329058 MS.gene44886:CDS 40.0%
AGTAGAGTCGATCTCAAATG+GGG + chr5.2:59329147-59329166 MS.gene44886:CDS 40.0%
CAATGCATATGTATCCCTAG+TGG + chr5.2:59329561-59329580 MS.gene44886:CDS 40.0%
CGAGTCAACAGATGATTACT+TGG + chr5.2:59328982-59329001 MS.gene44886:CDS 40.0%
CGTATATGGAATCCATCTAC+CGG + chr5.2:59328884-59328903 MS.gene44886:CDS 40.0%
GAATCTCTGCTTTCAATTCC+TGG + chr5.2:59329595-59329614 MS.gene44886:CDS 40.0%
GATGGTATGCTATCTCACTT+GGG + chr5.2:59329040-59329059 MS.gene44886:CDS 40.0%
GTTATTCAAGCCTCCGTATA+TGG + chr5.2:59328870-59328889 MS.gene44886:CDS 40.0%
TAGCAGGGCGTATGATTTAT+GGG + chr5.2:59329285-59329304 MS.gene44886:CDS 40.0%
TCTCATTGAGAGCTAATGTG+TGG + chr5.2:59329068-59329087 MS.gene44886:CDS 40.0%
TGACAGAACCAAGAAGAATG+AGG + chr5.2:59329645-59329664 MS.gene44886:CDS 40.0%
TGTTCTGCGAATAAGGCAAA+AGG - chr5.2:59329114-59329133 None:intergenic 40.0%
! ACTCTTGTCCTGTCTTTTGA+TGG + chr5.2:59329409-59329428 MS.gene44886:CDS 40.0%
! CGGTAGATGGATTCCATATA+CGG - chr5.2:59328886-59328905 None:intergenic 40.0%
! TAGATGGATTCCATATACGG+AGG - chr5.2:59328883-59328902 None:intergenic 40.0%
AAATGGGGCTATTCACTGGT+TGG + chr5.2:59329162-59329181 MS.gene44886:CDS 45.0%
AAGAAACATGCGTGGTGCAT+TGG + chr5.2:59329489-59329508 MS.gene44886:CDS 45.0%
AATGTGTGGAGCGCAATTGA+GGG + chr5.2:59329082-59329101 MS.gene44886:CDS 45.0%
ATAGAGGATGTCTCGCTCTA+TGG + chr5.2:59329311-59329330 MS.gene44886:CDS 45.0%
ATCGTTGGAAGAAACATGCG+TGG + chr5.2:59329481-59329500 MS.gene44886:CDS 45.0%
CAGGATTGCCATCAAAAGAC+AGG - chr5.2:59329420-59329439 None:intergenic 45.0%
CATAAATCATACGCCCTGCT+AGG - chr5.2:59329286-59329305 None:intergenic 45.0%
CGTTTGTGAAATCCGGTAGA+TGG - chr5.2:59328899-59328918 None:intergenic 45.0%
CTAGCAGGGCGTATGATTTA+TGG + chr5.2:59329284-59329303 MS.gene44886:CDS 45.0%
CTTGAGTGGCTGCATTTGTA+GGG + chr5.2:59329728-59329747 MS.gene44886:intron 45.0%
GATAGCATACCATCTGAGCT+CGG - chr5.2:59329034-59329053 None:intergenic 45.0%
TAATGTGTGGAGCGCAATTG+AGG + chr5.2:59329081-59329100 MS.gene44886:CDS 45.0%
TAGAGGATGTCTCGCTCTAT+GGG + chr5.2:59329312-59329331 MS.gene44886:CDS 45.0%
TCTCAAATGGGGCTATTCAC+TGG + chr5.2:59329158-59329177 MS.gene44886:CDS 45.0%
TCTCTGCTTTCAATTCCTGG+TGG + chr5.2:59329598-59329617 MS.gene44886:CDS 45.0%
TCTTGAGTGGCTGCATTTGT+AGG + chr5.2:59329727-59329746 MS.gene44886:intron 45.0%
CATCAGCTGATGTGCCATAC+TGG - chr5.2:59329786-59329805 None:intergenic 50.0%
TGAGCTCGGAGTCTGTTCAT+AGG - chr5.2:59329020-59329039 None:intergenic 50.0%
TTTGGCCAAAAGGAGTGAGC+AGG - chr5.2:59329439-59329458 None:intergenic 50.0%
! GCAATCCTGCTCACTCCTTT+TGG + chr5.2:59329431-59329450 MS.gene44886:CDS 50.0%
! TCAATTCCTGGTGGTGACAG+TGG + chr5.2:59329607-59329626 MS.gene44886:CDS 50.0%
ACTTGACCACTGTCACCACC+AGG - chr5.2:59329616-59329635 None:intergenic 55.0%
GAACAGACTCCGAGCTCAGA+TGG + chr5.2:59329022-59329041 MS.gene44886:CDS 55.0%
GTGTGGAGCGCAATTGAGGG+TGG + chr5.2:59329085-59329104 MS.gene44886:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 59328656 59330046 59328656 ID=MS.gene44886
chr5.2 mRNA 59328656 59330046 59328656 ID=MS.gene44886.t1;Parent=MS.gene44886
chr5.2 exon 59328656 59329666 59328656 ID=MS.gene44886.t1.exon1;Parent=MS.gene44886.t1
chr5.2 CDS 59328656 59329666 59328656 ID=cds.MS.gene44886.t1;Parent=MS.gene44886.t1
chr5.2 exon 59330032 59330046 59330032 ID=MS.gene44886.t1.exon2;Parent=MS.gene44886.t1
chr5.2 CDS 59330032 59330046 59330032 ID=cds.MS.gene44886.t1;Parent=MS.gene44886.t1
Gene Sequence

>MS.gene44886

ATGTTGATAACTCCATATCTCAAAGCTCTATCTATAGATCTTGAATCATCGCTTAACGATGATAGTGCTTCATATCCAATGGACATTAGTTTTTTGATTGACGATGATGATTACTATAGTTCTTCTTCATCTGACATGGATGAATTGTCGCCTCCCAAATCTTTTTTTAAACTTGATTTTAAAGGTTCATGCAGAGGCTTTATACTTTTGAATTGTTATTCAAGCCTCCGTATATGGAATCCATCTACCGGATTTCACAAACGAATACCTTTTACTACAATTGATTCCAATCCAGATGCAAATTATTTCTATGGTTTTGGGTATGACGAGTCAACAGATGATTACTTGGTGCTTTCAATGTCCTATGAACAGACTCCGAGCTCAGATGGTATGCTATCTCACTTGGGAATTTTCTCATTGAGAGCTAATGTGTGGAGCGCAATTGAGGGTGGTAACCTTTTGCCTTATTCGCAGAACAGTCTTTTAAACTTAGTAGAGTCGATCTCAAATGGGGCTATTCACTGGTTGGCTTTTCGTAATGATATATCAATGCCTGTTATTGTTGCCTTTCATTTAATTGAAAGGAAACTTCTAGAGTTGCGTTTGCCAAATGATATAATCAATGGTCCTAGCAGGGCGTATGATTTATGGGTGTATAGAGGATGTCTCGCTCTATGGGATATTTTGTCTGATAGAGTTACATTTGAAATATGGGTTATGGAAAAATACAATGTGCAATCGTCTTGGACTAAGACTCTTGTCCTGTCTTTTGATGGCAATCCTGCTCACTCCTTTTGGCCAAAATACTATACAAAAAGTGGTGATATCGTTGGAAGAAACATGCGTGGTGCATTGGCCAAGTATAATGACAAAGGACAGCTGCAAGAACATCACTCCTACTGTGACAATGCATATGTATCCCTAGTGGTTATGTATACAGAATCTCTGCTTTCAATTCCTGGTGGTGACAGTGGTCAAGTTCAAGAAGATGACAGAACCAAGAAGAATGAGGTTTTGAAGTATTCTTCTTCACCATTTTCATGATCTTTATAATTATATTGTATTATGTACTCTTGAGTGGCTGCATTTGTAGGGTAATATATGTTTGATCGCATTTGTAATATGATCCAGTATGGCACATCAGCTGATGAAACAAATGTCATGAAATGTTTACTGGTCAGACATTTGTATTGTGTTTATTGAAAATGTTGACTGCTTTGTTTCTATGGTTCAATGTTATTTGTGCATGTGTATGGATTGTATTTTAATGTATGTTTGTGGTCATTGGTTGAAATTGGTTTTATATTGTAATTGATTTTATGAACTTATGCATGTTGATCAATATCAAAATCTCTTTCTCATTTCATAAATCAAAGACCAACTCAGAGTAA
Protein sequence

>MS.gene44886.t1

MLITPYLKALSIDLESSLNDDSASYPMDISFLIDDDDYYSSSSSDMDELSPPKSFFKLDFKGSCRGFILLNCYSSLRIWNPSTGFHKRIPFTTIDSNPDANYFYGFGYDESTDDYLVLSMSYEQTPSSDGMLSHLGIFSLRANVWSAIEGGNLLPYSQNSLLNLVESISNGAIHWLAFRNDISMPVIVAFHLIERKLLELRLPNDIINGPSRAYDLWVYRGCLALWDILSDRVTFEIWVMEKYNVQSSWTKTLVLSFDGNPAHSFWPKYYTKSGDIVGRNMRGALAKYNDKGQLQEHHSYCDNAYVSLVVMYTESLLSIPGGDSGQVQEDDRTKKNETNSE