AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62398


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62398.t1 MTR_4g114700 65.089 169 19 1 1 169 90 218 6.24e-69 209
MS.gene62398.t1 MTR_4g117720 55.080 187 72 4 2 178 194 378 3.59e-61 194
MS.gene62398.t1 MTR_3g103020 54.787 188 69 5 2 178 196 378 1.87e-59 189
MS.gene62398.t1 MTR_4g116330 53.514 185 75 4 2 178 199 380 4.14e-59 189
MS.gene62398.t1 MTR_4g116320 54.348 184 71 6 1 176 78 256 7.57e-57 179
MS.gene62398.t1 MTR_4g109270 55.090 167 64 3 20 178 201 364 3.73e-56 181
MS.gene62398.t1 MTR_6g060650 52.747 182 75 3 2 177 196 372 6.10e-56 180
MS.gene62398.t1 MTR_3g034180 51.872 187 75 4 2 177 306 488 6.05e-55 181
MS.gene62398.t1 MTR_4g109280 51.913 183 79 4 2 178 180 359 6.85e-55 177
MS.gene62398.t1 MTR_8g040935 50.000 180 77 4 11 178 30 208 2.55e-53 169
MS.gene62398.t1 MTR_4g117850 51.075 186 76 6 1 175 195 376 2.09e-51 169
MS.gene62398.t1 MTR_3g034130 50.898 167 72 3 4 164 200 362 2.98e-50 166
MS.gene62398.t1 MTR_1g055175 47.283 184 84 6 4 178 22 201 1.02e-48 157
MS.gene62398.t1 MTR_3g034160 49.721 179 79 3 5 177 201 374 3.43e-48 160
MS.gene62398.t1 MTR_1g055165 48.555 173 77 5 14 178 150 318 5.42e-46 153
MS.gene62398.t1 MTR_4g023130 49.180 183 75 6 2 175 137 310 1.02e-45 152
MS.gene62398.t1 MTR_4g023380 44.498 209 76 6 2 178 179 379 3.64e-45 152
MS.gene62398.t1 MTR_4g023080 48.634 183 76 6 2 175 178 351 6.94e-45 151
MS.gene62398.t1 MTR_4g023090 45.000 200 78 7 2 177 194 385 1.79e-44 151
MS.gene62398.t1 MTR_4g021785 47.312 186 83 5 1 178 177 355 2.02e-44 150
MS.gene62398.t1 MTR_4g118370 46.328 177 77 5 1 175 97 257 3.37e-44 147
MS.gene62398.t1 MTR_4g094365 48.677 189 77 8 2 178 184 364 5.13e-44 149
MS.gene62398.t1 MTR_5g097210 47.727 176 80 5 8 176 191 361 6.47e-44 149
MS.gene62398.t1 MTR_4g118380 48.765 162 66 3 2 148 198 357 2.07e-43 147
MS.gene62398.t1 MTR_8g433040 47.778 180 81 6 2 178 195 364 4.14e-43 147
MS.gene62398.t1 MTR_8g032380 47.778 180 81 6 2 178 195 364 4.14e-43 147
MS.gene62398.t1 MTR_8g433030 48.087 183 76 8 2 173 163 337 4.30e-42 144
MS.gene62398.t1 MTR_4g122850 45.902 183 85 8 5 178 187 364 4.36e-42 145
MS.gene62398.t1 MTR_8g032390 48.087 183 76 8 2 173 196 370 1.11e-41 143
MS.gene62398.t1 MTR_4g117870 45.506 178 62 5 2 178 124 267 1.79e-41 140
MS.gene62398.t1 MTR_1g055195 48.193 166 73 5 21 178 318 478 4.37e-41 144
MS.gene62398.t1 MTR_4g023070 45.918 196 75 8 2 175 181 367 1.08e-39 138
MS.gene62398.t1 MTR_4g117770 44.149 188 90 5 2 178 184 367 1.52e-39 138
MS.gene62398.t1 MTR_4g117810 44.149 188 90 5 2 178 184 367 1.52e-39 138
MS.gene62398.t1 MTR_5g070130 46.632 193 84 10 2 178 188 377 2.59e-39 137
MS.gene62398.t1 MTR_4g118360 53.488 129 55 3 52 178 1 126 3.83e-39 130
MS.gene62398.t1 MTR_1g055135 47.305 167 73 5 23 178 213 375 7.00e-39 136
MS.gene62398.t1 MTR_4g023200 45.699 186 81 7 2 175 180 357 2.30e-38 134
MS.gene62398.t1 MTR_4g117780 44.149 188 90 5 2 178 184 367 3.37e-38 134
MS.gene62398.t1 MTR_4g116330 44.693 179 61 4 2 178 199 341 8.39e-38 132
MS.gene62398.t1 MTR_4g023190 45.161 186 81 7 2 175 180 356 8.92e-38 133
MS.gene62398.t1 MTR_4g023150 44.624 186 83 7 2 175 180 357 1.05e-37 133
MS.gene62398.t1 MTR_6g463430 43.716 183 85 6 2 175 26 199 5.41e-37 127
MS.gene62398.t1 MTR_4g023120 44.103 195 80 7 2 175 181 367 5.44e-37 131
MS.gene62398.t1 MTR_5g070080 49.398 166 71 5 22 178 225 386 1.43e-36 130
MS.gene62398.t1 MTR_4g117750 47.305 167 70 4 21 174 194 355 1.86e-36 132
MS.gene62398.t1 MTR_4g117750 48.148 135 57 4 51 175 349 480 2.93e-32 120
MS.gene62398.t1 MTR_4g118400 42.697 178 79 6 1 172 134 294 2.06e-36 128
MS.gene62398.t1 MTR_4g023440 44.633 177 61 5 2 178 193 332 1.63e-35 126
MS.gene62398.t1 MTR_5g070088 47.742 155 69 4 28 173 229 380 3.20e-35 127
MS.gene62398.t1 MTR_1g055250 43.421 152 76 4 3 146 90 239 8.72e-34 122
MS.gene62398.t1 MTR_8g102520 43.860 171 84 4 16 178 156 322 1.47e-33 121
MS.gene62398.t1 MTR_7g079400 42.932 191 91 7 2 178 151 337 5.89e-33 120
MS.gene62398.t1 MTR_5g023000 61.856 97 32 2 83 178 7 99 9.45e-33 113
MS.gene62398.t1 MTR_4g117530 39.326 178 76 4 2 178 107 253 8.64e-32 115
MS.gene62398.t1 MTR_4g122860 40.884 181 76 7 4 178 165 320 1.05e-28 108
MS.gene62398.t1 MTR_7g079370 40.000 180 95 6 2 172 175 350 1.52e-28 108
MS.gene62398.t1 MTR_7g079420 40.323 186 90 8 7 178 966 1144 5.15e-27 107
MS.gene62398.t1 MTR_7g079450 44.286 140 72 3 39 178 4 137 1.59e-26 98.2
MS.gene62398.t1 MTR_8g098830 43.704 135 65 4 18 144 63 194 8.76e-25 96.3
MS.gene62398.t1 MTR_4g121580 35.928 167 76 6 5 168 201 339 1.37e-22 93.2
MS.gene62398.t1 MTR_7g117720 55.172 87 38 1 88 173 186 272 4.72e-20 84.7
MS.gene62398.t1 MTR_4g023430 48.148 81 42 0 98 178 142 222 1.49e-19 82.4
MS.gene62398.t1 MTR_4g122840 38.849 139 44 5 43 178 164 264 3.56e-17 77.0
MS.gene62398.t1 MTR_2g048580 31.771 192 99 8 2 178 168 342 7.39e-17 77.0
MS.gene62398.t1 MTR_8g013005 54.839 62 25 1 117 178 1 59 9.14e-16 68.2
MS.gene62398.t1 MTR_8g013010 46.512 86 34 3 2 81 235 314 3.92e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTGTATACAACGCAAAT+TGG 0.229651 8.2:+13955958 None:intergenic
AGAGTAGGGTTGGTCTTTAA+TGG 0.230123 8.2:-13956371 MS.gene62398:CDS
GGTACTTGATATCAGGAAAA+TGG 0.231212 8.2:+13956411 None:intergenic
TTAGAGAATACTTTAGTATA+TGG 0.244586 8.2:-13956226 MS.gene62398:CDS
ATTCTTATTCTTTCTCTTGA+TGG 0.256363 8.2:-13956134 MS.gene62398:CDS
TTCTAGAGCATGACTCATTA+TGG 0.262944 8.2:-13956007 MS.gene62398:CDS
CGATTATGGTCAAAATCATT+TGG 0.310127 8.2:+13956272 None:intergenic
TCTTTAATGGGTCTATTCAT+TGG 0.374417 8.2:-13956358 MS.gene62398:CDS
TAGAGAATACTTTAGTATAT+GGG 0.376537 8.2:-13956225 MS.gene62398:CDS
GAGTAGGGTTGGTCTTTAAT+GGG 0.380532 8.2:-13956370 MS.gene62398:CDS
GATGATCCATTACTTTCAAC+TGG 0.401175 8.2:-13955984 MS.gene62398:CDS
TTGGGACGGTACTTGATATC+AGG 0.417403 8.2:+13956404 None:intergenic
CTCTTGATGGCATCCCCTCT+CGG 0.431495 8.2:-13956121 MS.gene62398:CDS
TTAGGCTTGCATGTTGTCAC+CGG 0.436965 8.2:+13955920 None:intergenic
TTCTGTATACAACGCAAATT+GGG 0.440787 8.2:+13955959 None:intergenic
GGTATCATACACTTGAGATA+TGG 0.444256 8.2:-13956196 MS.gene62398:CDS
GTTGGAAGAGATGACAAAAC+TGG 0.445047 8.2:-13956059 MS.gene62398:CDS
TGGTCAAAATCATTTGGAAA+AGG 0.452962 8.2:+13956278 None:intergenic
TACTTTAGTATATGGGCTAT+GGG 0.453026 8.2:-13956218 MS.gene62398:CDS
TCTAGAGCATGACTCATTAT+GGG 0.456461 8.2:-13956006 MS.gene62398:CDS
AATACAAAGTACAATCATCT+TGG 0.466827 8.2:-13956163 MS.gene62398:CDS
ATACTTTAGTATATGGGCTA+TGG 0.472579 8.2:-13956219 MS.gene62398:CDS
GTACTTGATATCAGGAAAAT+GGG 0.474629 8.2:+13956412 None:intergenic
GAATCTTTGCTTTCACTCCC+CGG 0.486445 8.2:-13955939 MS.gene62398:CDS
CCCTACTCTAGGCTCAGGAT+TGG 0.512525 8.2:+13956385 None:intergenic
ACCAACCCTACTCTAGGCTC+AGG 0.522644 8.2:+13956380 None:intergenic
CCTACTCTAGGCTCAGGATT+GGG 0.526604 8.2:+13956386 None:intergenic
TAGGCTTGCATGTTGTCACC+GGG 0.528934 8.2:+13955921 None:intergenic
TGGGATCCAGTTGAAAGTAA+TGG 0.537735 8.2:+13955978 None:intergenic
TCCTGAGCCTAGAGTAGGGT+TGG 0.540414 8.2:-13956381 MS.gene62398:CDS
TTAAAGACCAACCCTACTCT+AGG 0.572534 8.2:+13956374 None:intergenic
CCAATCCTGAGCCTAGAGTA+GGG 0.576939 8.2:-13956385 MS.gene62398:CDS
ATTGGGATAAAGTGCCGAGA+GGG 0.579058 8.2:+13956107 None:intergenic
CCCAATCCTGAGCCTAGAGT+AGG 0.582388 8.2:-13956386 MS.gene62398:CDS
TAATATGTGGCATGTAATCG+AGG 0.602408 8.2:-13956438 None:intergenic
ATGCTGAAGTATAATTACAA+AGG 0.616769 8.2:-13956035 MS.gene62398:CDS
AATATGTGGCATGTAATCGA+GGG 0.644013 8.2:-13956437 MS.gene62398:CDS
CTCTAGGCTCAGGATTGGGA+CGG 0.645228 8.2:+13956390 None:intergenic
TTGGGATAAAGTGCCGAGAG+GGG 0.664166 8.2:+13956108 None:intergenic
ACTTTAGTATATGGGCTATG+GGG 0.671907 8.2:-13956217 MS.gene62398:CDS
AGGCTTGCATGTTGTCACCG+GGG 0.724966 8.2:+13955922 None:intergenic
TATTGGGATAAAGTGCCGAG+AGG 0.743389 8.2:+13956106 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGAGAATACTTTAGTATAT+GGG - chr8.2:13956129-13956148 MS.gene62398:CDS 20.0%
!! TTAGAGAATACTTTAGTATA+TGG - chr8.2:13956128-13956147 MS.gene62398:CDS 20.0%
! AATACAAAGTACAATCATCT+TGG - chr8.2:13956191-13956210 MS.gene62398:CDS 25.0%
! ATGCTGAAGTATAATTACAA+AGG - chr8.2:13956319-13956338 MS.gene62398:CDS 25.0%
! ATTCTTATTCTTTCTCTTGA+TGG - chr8.2:13956220-13956239 MS.gene62398:CDS 25.0%
!! AATCACATTTTTCACGATTA+TGG + chr8.2:13956099-13956118 None:intergenic 25.0%
!!! CACTTTTTGTTGAACATATT+GGG + chr8.2:13956267-13956286 None:intergenic 25.0%
ATCGTGAAAAATGTGATTTG+TGG - chr8.2:13956101-13956120 MS.gene62398:CDS 30.0%
ATTCTGTATACAACGCAAAT+TGG + chr8.2:13956399-13956418 None:intergenic 30.0%
CCAATATGTTCAACAAAAAG+TGG - chr8.2:13956265-13956284 MS.gene62398:CDS 30.0%
CGATTATGGTCAAAATCATT+TGG + chr8.2:13956085-13956104 None:intergenic 30.0%
GTACTTGATATCAGGAAAAT+GGG + chr8.2:13955945-13955964 None:intergenic 30.0%
TGGTCAAAATCATTTGGAAA+AGG + chr8.2:13956079-13956098 None:intergenic 30.0%
TTCTGTATACAACGCAAATT+GGG + chr8.2:13956398-13956417 None:intergenic 30.0%
! ATACTTTAGTATATGGGCTA+TGG - chr8.2:13956135-13956154 MS.gene62398:CDS 30.0%
! TACTTTAGTATATGGGCTAT+GGG - chr8.2:13956136-13956155 MS.gene62398:CDS 30.0%
! TCGTGAAAAATGTGATTTGT+GGG - chr8.2:13956102-13956121 MS.gene62398:CDS 30.0%
! TCTTTAATGGGTCTATTCAT+TGG - chr8.2:13955996-13956015 MS.gene62398:CDS 30.0%
!!! CCACTTTTTGTTGAACATAT+TGG + chr8.2:13956268-13956287 None:intergenic 30.0%
ACAAAAAGTGGTGACATTGT+TGG - chr8.2:13956277-13956296 MS.gene62398:CDS 35.0%
GATGATCCATTACTTTCAAC+TGG - chr8.2:13956370-13956389 MS.gene62398:CDS 35.0%
GGTACTTGATATCAGGAAAA+TGG + chr8.2:13955946-13955965 None:intergenic 35.0%
GGTATCATACACTTGAGATA+TGG - chr8.2:13956158-13956177 MS.gene62398:CDS 35.0%
! ACTTTAGTATATGGGCTATG+GGG - chr8.2:13956137-13956156 MS.gene62398:CDS 35.0%
! TCTAGAGCATGACTCATTAT+GGG - chr8.2:13956348-13956367 MS.gene62398:CDS 35.0%
!! TTCTAGAGCATGACTCATTA+TGG - chr8.2:13956347-13956366 MS.gene62398:CDS 35.0%
GTTGGAAGAGATGACAAAAC+TGG - chr8.2:13956295-13956314 MS.gene62398:CDS 40.0%
TGGGATCCAGTTGAAAGTAA+TGG + chr8.2:13956379-13956398 None:intergenic 40.0%
TTAAAGACCAACCCTACTCT+AGG + chr8.2:13955983-13956002 None:intergenic 40.0%
!! AGAGTAGGGTTGGTCTTTAA+TGG - chr8.2:13955983-13956002 MS.gene62398:CDS 40.0%
!! GAGTAGGGTTGGTCTTTAAT+GGG - chr8.2:13955984-13956003 MS.gene62398:CDS 40.0%
GAATCTTTGCTTTCACTCCC+CGG - chr8.2:13956415-13956434 MS.gene62398:CDS 45.0%
TTAGGCTTGCATGTTGTCAC+CGG + chr8.2:13956437-13956456 None:intergenic 45.0%
TTGGGACGGTACTTGATATC+AGG + chr8.2:13955953-13955972 None:intergenic 45.0%
!! ATTGGGATAAAGTGCCGAGA+GGG + chr8.2:13956250-13956269 None:intergenic 45.0%
!! TATTGGGATAAAGTGCCGAG+AGG + chr8.2:13956251-13956270 None:intergenic 45.0%
CCAATCCTGAGCCTAGAGTA+GGG - chr8.2:13955969-13955988 MS.gene62398:CDS 50.0%
CCTACTCTAGGCTCAGGATT+GGG + chr8.2:13955971-13955990 None:intergenic 50.0%
TAGGCTTGCATGTTGTCACC+GGG + chr8.2:13956436-13956455 None:intergenic 50.0%
!! TTGGGATAAAGTGCCGAGAG+GGG + chr8.2:13956249-13956268 None:intergenic 50.0%
ACCAACCCTACTCTAGGCTC+AGG + chr8.2:13955977-13955996 None:intergenic 55.0%
AGGCTTGCATGTTGTCACCG+GGG + chr8.2:13956435-13956454 None:intergenic 55.0%
CCCAATCCTGAGCCTAGAGT+AGG - chr8.2:13955968-13955987 MS.gene62398:CDS 55.0%
CCCTACTCTAGGCTCAGGAT+TGG + chr8.2:13955972-13955991 None:intergenic 55.0%
CTCTAGGCTCAGGATTGGGA+CGG + chr8.2:13955967-13955986 None:intergenic 55.0%
TCCTGAGCCTAGAGTAGGGT+TGG - chr8.2:13955973-13955992 MS.gene62398:CDS 55.0%
! CTCTTGATGGCATCCCCTCT+CGG - chr8.2:13956233-13956252 MS.gene62398:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 13955920 13956456 13955920 ID=MS.gene62398
chr8.2 mRNA 13955920 13956456 13955920 ID=MS.gene62398.t1;Parent=MS.gene62398
chr8.2 exon 13955920 13956456 13955920 ID=MS.gene62398.t1.exon1;Parent=MS.gene62398.t1
chr8.2 CDS 13955920 13956456 13955920 ID=cds.MS.gene62398.t1;Parent=MS.gene62398.t1
Gene Sequence

>MS.gene62398

ATGTGGCATGTAATCGAGGGTACCCATTTTCCTGATATCAAGTACCGTCCCAATCCTGAGCCTAGAGTAGGGTTGGTCTTTAATGGGTCTATTCATTGGTCATCGATGTATGATATTCTTGCATTTGATTTAATTGAAAGAAAACTTTTAGTGATGCCTTTTCCAAATGATTTTGACCATAATCGTGAAAAATGTGATTTGTGGGTATTTAGAGAATACTTTAGTATATGGGCTATGGGGTATCATACACTTGAGATATGGATGATGAAAGAATACAAAGTACAATCATCTTGGACTAAGATTCTTATTCTTTCTCTTGATGGCATCCCCTCTCGGCACTTTATCCCAATATGTTCAACAAAAAGTGGTGACATTGTTGGAAGAGATGACAAAACTGGAATGCTGAAGTATAATTACAAAGGAGAGCTTCTAGAGCATGACTCATTATGGGATGATCCATTACTTTCAACTGGATCCCAATTTGCGTTGTATACAGAATCTTTGCTTTCACTCCCCGGTGACAACATGCAAGCCTAA

Protein sequence

>MS.gene62398.t1

MWHVIEGTHFPDIKYRPNPEPRVGLVFNGSIHWSSMYDILAFDLIERKLLVMPFPNDFDHNREKCDLWVFREYFSIWAMGYHTLEIWMMKEYKVQSSWTKILILSLDGIPSRHFIPICSTKSGDIVGRDDKTGMLKYNYKGELLEHDSLWDDPLLSTGSQFALYTESLLSLPGDNMQA