AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063670.t1 MTR_4g117720 63.298 188 64 4 6 192 159 342 5.45e-75 230
MS.gene063670.t1 MTR_6g060650 61.170 188 67 5 6 192 161 343 8.70e-73 224
MS.gene063670.t1 MTR_3g103020 61.257 191 67 5 6 192 161 348 3.55e-72 223
MS.gene063670.t1 MTR_4g109270 62.567 187 62 4 6 191 154 333 5.37e-69 214
MS.gene063670.t1 MTR_4g116330 55.080 187 80 3 6 192 166 348 5.00e-68 212
MS.gene063670.t1 MTR_3g034130 54.545 198 75 5 6 192 153 346 2.08e-67 210
MS.gene063670.t1 MTR_4g117850 55.208 192 80 3 6 192 159 349 4.02e-65 205
MS.gene063670.t1 MTR_4g109280 62.195 164 57 4 29 192 169 327 8.28e-64 201
MS.gene063670.t1 MTR_8g040935 55.932 177 75 3 18 192 1 176 2.12e-63 195
MS.gene063670.t1 MTR_3g034180 55.051 198 73 6 6 192 267 459 6.65e-63 202
MS.gene063670.t1 MTR_4g023130 56.150 187 71 5 6 191 108 284 9.45e-63 197
MS.gene063670.t1 MTR_4g117870 55.851 188 43 4 6 192 89 237 4.62e-62 193
MS.gene063670.t1 MTR_4g118380 56.345 197 71 6 6 192 163 354 5.66e-62 196
MS.gene063670.t1 MTR_4g116320 55.263 190 75 5 7 192 43 226 2.10e-61 191
MS.gene063670.t1 MTR_3g034160 56.566 198 71 6 6 192 153 346 9.83e-61 193
MS.gene063670.t1 MTR_4g023080 54.545 187 74 5 6 191 149 325 2.93e-60 191
MS.gene063670.t1 MTR_4g117770 55.556 189 76 5 6 191 153 336 3.74e-60 192
MS.gene063670.t1 MTR_4g117810 55.556 189 76 5 6 191 153 336 3.74e-60 192
MS.gene063670.t1 MTR_4g094365 53.439 189 80 5 6 192 152 334 1.21e-59 190
MS.gene063670.t1 MTR_8g433030 53.968 189 83 4 6 192 128 314 1.25e-59 189
MS.gene063670.t1 MTR_4g117780 54.497 189 78 5 6 191 153 336 1.92e-59 190
MS.gene063670.t1 MTR_8g032390 53.968 189 83 4 6 192 161 347 2.92e-59 189
MS.gene063670.t1 MTR_4g021785 53.226 186 79 4 6 191 149 326 3.08e-58 186
MS.gene063670.t1 MTR_4g023090 49.751 201 82 5 6 192 161 356 2.05e-56 182
MS.gene063670.t1 MTR_4g023070 49.754 203 81 5 6 191 143 341 1.92e-55 180
MS.gene063670.t1 MTR_4g023120 49.754 203 81 6 6 191 143 341 1.64e-54 177
MS.gene063670.t1 MTR_4g122850 51.562 192 84 8 6 192 147 334 1.67e-54 177
MS.gene063670.t1 MTR_6g463430 53.933 178 71 5 18 191 1 171 1.01e-53 170
MS.gene063670.t1 MTR_5g097210 50.000 192 86 6 6 192 147 333 1.84e-51 169
MS.gene063670.t1 MTR_4g023380 47.368 209 68 5 6 192 161 349 2.53e-51 169
MS.gene063670.t1 MTR_5g070130 48.718 195 91 5 6 192 153 346 3.32e-51 169
MS.gene063670.t1 MTR_5g070080 49.468 188 91 4 6 192 171 355 1.61e-50 167
MS.gene063670.t1 MTR_1g055250 47.917 192 89 7 6 192 53 238 1.66e-49 163
MS.gene063670.t1 MTR_8g433040 50.526 190 78 5 6 192 160 336 2.71e-49 164
MS.gene063670.t1 MTR_8g032380 50.526 190 78 5 6 192 160 336 2.71e-49 164
MS.gene063670.t1 MTR_4g023190 52.332 193 74 7 6 192 151 331 2.59e-48 161
MS.gene063670.t1 MTR_4g023200 50.262 191 82 6 6 192 151 332 5.96e-47 157
MS.gene063670.t1 MTR_4g118400 53.614 166 60 5 27 192 122 270 6.72e-47 155
MS.gene063670.t1 MTR_4g118370 47.938 194 70 8 6 192 61 230 8.34e-47 154
MS.gene063670.t1 MTR_1g055195 47.959 196 80 10 6 192 266 448 1.87e-46 159
MS.gene063670.t1 MTR_1g055175 49.711 173 78 7 24 192 3 170 2.68e-46 151
MS.gene063670.t1 MTR_4g023150 50.259 193 79 7 6 192 151 332 3.06e-46 155
MS.gene063670.t1 MTR_4g116330 45.455 187 59 3 6 192 166 309 4.07e-46 155
MS.gene063670.t1 MTR_5g070088 46.073 191 97 4 6 192 167 355 9.83e-45 152
MS.gene063670.t1 MTR_4g117530 45.989 187 63 4 6 192 78 226 3.61e-44 147
MS.gene063670.t1 MTR_1g055135 45.918 196 93 7 6 192 153 344 3.85e-44 150
MS.gene063670.t1 MTR_4g121580 48.936 188 62 6 6 189 161 318 7.20e-43 147
MS.gene063670.t1 MTR_5g023000 95.652 69 3 0 124 192 1 69 2.69e-42 137
MS.gene063670.t1 MTR_4g117750 48.515 202 81 11 6 192 136 329 8.01e-41 144
MS.gene063670.t1 MTR_4g117750 57.692 104 36 1 97 192 350 453 3.23e-30 115
MS.gene063670.t1 MTR_4g023440 44.385 187 62 3 6 192 160 304 2.48e-40 139
MS.gene063670.t1 MTR_7g079400 44.330 194 95 8 6 192 115 302 8.30e-39 135
MS.gene063670.t1 MTR_1g055165 51.825 137 60 5 57 192 156 287 4.68e-37 130
MS.gene063670.t1 MTR_8g098830 46.707 167 77 8 30 191 35 194 4.36e-36 126
MS.gene063670.t1 MTR_7g079370 43.655 197 96 8 5 192 135 325 5.12e-36 129
MS.gene063670.t1 MTR_4g117430 55.462 119 44 4 6 124 46 155 5.34e-35 121
MS.gene063670.t1 MTR_8g102520 41.026 195 100 8 6 192 104 291 1.69e-34 124
MS.gene063670.t1 MTR_8g013010 54.472 123 48 5 6 127 200 315 1.95e-34 124
MS.gene063670.t1 MTR_4g118360 71.084 83 20 2 110 192 16 94 1.83e-32 113
MS.gene063670.t1 MTR_4g122860 41.718 163 61 5 35 192 157 290 5.66e-30 112
MS.gene063670.t1 MTR_4g114700 37.037 189 68 3 6 192 55 194 2.23e-27 103
MS.gene063670.t1 MTR_7g079420 35.567 194 82 9 6 192 956 1113 2.37e-22 94.4
MS.gene063670.t1 MTR_7g079450 47.748 111 52 3 82 192 2 106 6.82e-22 87.0
MS.gene063670.t1 MTR_2g048580 36.905 168 89 8 30 192 158 313 3.24e-21 89.4
MS.gene063670.t1 MTR_4g116340 53.012 83 35 4 6 87 168 247 2.19e-18 80.5
MS.gene063670.t1 MTR_6g034720 41.085 129 65 6 6 129 77 199 1.26e-17 77.8
MS.gene063670.t1 MTR_7g117720 68.966 58 17 1 135 191 186 243 2.63e-17 77.8
MS.gene063670.t1 MTR_4g133170 31.551 187 114 8 6 185 147 326 1.45e-16 77.0
MS.gene063670.t1 MTR_2g028160 35.762 151 82 7 29 170 194 338 4.21e-16 75.9
MS.gene063670.t1 MTR_2g028130 30.729 192 117 7 6 187 168 353 4.33e-16 75.5
MS.gene063670.t1 MTR_2g426020 28.342 187 121 6 6 187 165 343 7.40e-16 75.1
MS.gene063670.t1 MTR_2g025960 30.114 176 112 6 6 176 73 242 2.99e-15 72.8
MS.gene063670.t1 MTR_0667s0010 29.508 183 111 7 6 176 157 333 5.75e-15 72.4
MS.gene063670.t1 MTR_2g026010 30.348 201 111 9 6 190 143 330 9.92e-14 68.9
MS.gene063670.t1 MTR_8g099460 29.834 181 110 7 6 182 158 325 1.28e-13 68.6
MS.gene063670.t1 MTR_2g425980 31.658 199 117 8 6 191 163 355 4.77e-13 67.0
MS.gene063670.t1 MTR_1g068860 29.012 162 103 6 6 160 146 302 9.80e-13 65.9
MS.gene063670.t1 MTR_2g025420 29.518 166 100 6 29 183 197 356 1.34e-12 65.5
MS.gene063670.t1 MTR_2g021050 28.218 202 124 8 6 192 135 330 3.43e-12 64.3
MS.gene063670.t1 MTR_1g068875 28.421 190 120 9 12 191 150 333 3.47e-12 64.3
MS.gene063670.t1 MTR_3g083010 28.497 193 121 7 6 187 173 359 9.23e-12 63.2
MS.gene063670.t1 MTR_4g023430 57.143 49 21 0 144 192 141 189 2.78e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063670.t1 AT3G06240 28.177 181 111 6 10 185 222 388 8.56e-12 63.5

Find 34 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTTAGTATTAGCTAATT+TGG 0.070994 5.3:-13378429 None:intergenic
ACTTGTGATGTGTGGGTATT+TGG 0.192835 5.3:+13378689 MS.gene063670:CDS
ACTGATTACCCTAAAATAAT+AGG 0.192838 5.3:+13378527 MS.gene063670:CDS
TTGGAGAATTTCTCAGTATA+TGG 0.237144 5.3:+13378708 MS.gene063670:CDS
TGCTTAGTATTAGCTAATTT+GGG 0.241281 5.3:-13378428 None:intergenic
GTCAACAGATGATTACTTCT+TGG 0.329019 5.3:+13378388 MS.gene063670:CDS
CTAATGATACAGTTGAAATA+TGG 0.329864 5.3:+13378744 MS.gene063670:CDS
ATTAGCTAATACTAAGCATT+TGG 0.343978 5.3:+13378433 MS.gene063670:CDS
TCAGTAGAGGAATTGGTATA+AGG 0.366239 5.3:-13378509 None:intergenic
AGGGTAATCAGTAGAGGAAT+TGG 0.367467 5.3:-13378516 None:intergenic
CTGATTACCCTAAAATAATA+GGG 0.399318 5.3:+13378528 MS.gene063670:CDS
TGGAGAATTTCTCAGTATAT+GGG 0.404173 5.3:+13378709 MS.gene063670:CDS
CAGTAGAGGAATTGGTATAA+GGG 0.429509 5.3:-13378508 None:intergenic
TTGCCTTTGACTTAATCAAA+AGG 0.429640 5.3:+13378618 MS.gene063670:CDS
GCTTCATAGTCAATATCAAA+TGG 0.437341 5.3:-13378662 None:intergenic
AATACGAAGTGCATTCGTCT+TGG 0.467526 5.3:+13378777 MS.gene063670:CDS
TGCCTTTGACTTAATCAAAA+GGG 0.476859 5.3:+13378619 MS.gene063670:CDS
ATTTCTCAGTATATGGGCTA+TGG 0.479112 5.3:+13378715 MS.gene063670:CDS
GATATCATTGGAACAGAAGA+TGG 0.479823 5.3:+13378875 MS.gene063670:CDS
TAATACATGGAAAGAAATTG+AGG 0.507096 5.3:+13378475 MS.gene063670:CDS
TATAGGGGAAAATAGCTTAG+TGG 0.510161 5.3:-13378834 None:intergenic
TCTCATTGAGAGCTAATACA+TGG 0.515391 5.3:+13378462 MS.gene063670:CDS
CTTCATAGTCAATATCAAAT+GGG 0.517578 5.3:-13378661 None:intergenic
AACAGAAGATGGATGGAGAT+TGG 0.542542 5.3:+13378886 MS.gene063670:CDS
TAATGATACAGTTGAAATAT+GGG 0.542942 5.3:+13378745 MS.gene063670:CDS
TCATTGGAACAGAAGATGGA+TGG 0.561647 5.3:+13378879 MS.gene063670:CDS
TCGTCATGATCATTTACGAA+AGG 0.567831 5.3:+13378586 MS.gene063670:CDS
AATACATGGAAAGAAATTGA+GGG 0.567862 5.3:+13378476 MS.gene063670:CDS
TAGTCAATATCAAATGGGAG+AGG 0.576315 5.3:-13378656 None:intergenic
GCTTAGTATTAGCTAATTTG+GGG 0.598307 5.3:-13378427 None:intergenic
AGTCAATATCAAATGGGAGA+GGG 0.616754 5.3:-13378655 None:intergenic
AGCTACAACTTGTGATGTGT+GGG 0.618454 5.3:+13378682 MS.gene063670:CDS
AAGCTACAACTTGTGATGTG+TGG 0.628824 5.3:+13378681 MS.gene063670:CDS
TTGGTGAAGTATAATGACAA+AGG 0.639076 5.3:+13378905 MS.gene063670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGATACAGTTGAAATAT+GGG + chr5.3:13378745-13378764 MS.gene063670:CDS 20.0%
! AATACATGGAAAGAAATTGA+GGG + chr5.3:13378476-13378495 MS.gene063670:CDS 25.0%
! ACTGATTACCCTAAAATAAT+AGG + chr5.3:13378527-13378546 MS.gene063670:CDS 25.0%
! ATGCTTAGTATTAGCTAATT+TGG - chr5.3:13378432-13378451 None:intergenic 25.0%
! ATTAGCTAATACTAAGCATT+TGG + chr5.3:13378433-13378452 MS.gene063670:CDS 25.0%
! CTAATGATACAGTTGAAATA+TGG + chr5.3:13378744-13378763 MS.gene063670:CDS 25.0%
! CTGATTACCCTAAAATAATA+GGG + chr5.3:13378528-13378547 MS.gene063670:CDS 25.0%
! CTTCATAGTCAATATCAAAT+GGG - chr5.3:13378664-13378683 None:intergenic 25.0%
! TAATACATGGAAAGAAATTG+AGG + chr5.3:13378475-13378494 MS.gene063670:CDS 25.0%
! TGCTTAGTATTAGCTAATTT+GGG - chr5.3:13378431-13378450 None:intergenic 25.0%
!! AAAAGAGACCCTATTATTTT+AGG - chr5.3:13378539-13378558 None:intergenic 25.0%
!! ACTTTTTGTAGAACATATAG+GGG - chr5.3:13378852-13378871 None:intergenic 25.0%
!! ATAATAGGGTCTCTTTTTAA+TGG + chr5.3:13378542-13378561 MS.gene063670:CDS 25.0%
!! TAATAGGGTCTCTTTTTAAT+GGG + chr5.3:13378543-13378562 MS.gene063670:CDS 25.0%
!!! AAAGAGACCCTATTATTTTA+GGG - chr5.3:13378538-13378557 None:intergenic 25.0%
!!! CACTTTTTGTAGAACATATA+GGG - chr5.3:13378853-13378872 None:intergenic 25.0%
ACAAAAAGTGGTGATATCAT+TGG + chr5.3:13378863-13378882 MS.gene063670:CDS 30.0%
CCTATATGTTCTACAAAAAG+TGG + chr5.3:13378851-13378870 MS.gene063670:CDS 30.0%
GCTTAGTATTAGCTAATTTG+GGG - chr5.3:13378430-13378449 None:intergenic 30.0%
GCTTCATAGTCAATATCAAA+TGG - chr5.3:13378665-13378684 None:intergenic 30.0%
TGGAGAATTTCTCAGTATAT+GGG + chr5.3:13378709-13378728 MS.gene063670:CDS 30.0%
TTGGAGAATTTCTCAGTATA+TGG + chr5.3:13378708-13378727 MS.gene063670:CDS 30.0%
TTGGTGAAGTATAATGACAA+AGG + chr5.3:13378905-13378924 MS.gene063670:CDS 30.0%
! AATAGGGTCTCTTTTTAATG+GGG + chr5.3:13378544-13378563 MS.gene063670:CDS 30.0%
! TGCCTTTGACTTAATCAAAA+GGG + chr5.3:13378619-13378638 MS.gene063670:CDS 30.0%
! TTGCCTTTGACTTAATCAAA+AGG + chr5.3:13378618-13378637 MS.gene063670:CDS 30.0%
! TTTTTAATGGGGCTATTCAT+TGG + chr5.3:13378555-13378574 MS.gene063670:CDS 30.0%
!! TTCCCTTTTGATTAAGTCAA+AGG - chr5.3:13378624-13378643 None:intergenic 30.0%
!!! ACTCTTGTTTTGTCTATTGA+CGG + chr5.3:13378806-13378825 MS.gene063670:CDS 30.0%
!!! CCACTTTTTGTAGAACATAT+AGG - chr5.3:13378854-13378873 None:intergenic 30.0%
!!! TATTTTAGGGTAATCAGTAG+AGG - chr5.3:13378525-13378544 None:intergenic 30.0%
AGTCAATATCAAATGGGAGA+GGG - chr5.3:13378658-13378677 None:intergenic 35.0%
CAGTAGAGGAATTGGTATAA+GGG - chr5.3:13378511-13378530 None:intergenic 35.0%
GATATCATTGGAACAGAAGA+TGG + chr5.3:13378875-13378894 MS.gene063670:CDS 35.0%
GTCAACAGATGATTACTTCT+TGG + chr5.3:13378388-13378407 MS.gene063670:CDS 35.0%
TAGTCAATATCAAATGGGAG+AGG - chr5.3:13378659-13378678 None:intergenic 35.0%
TATAGGGGAAAATAGCTTAG+TGG - chr5.3:13378837-13378856 None:intergenic 35.0%
TCAGTAGAGGAATTGGTATA+AGG - chr5.3:13378512-13378531 None:intergenic 35.0%
TCGTCATGATCATTTACGAA+AGG + chr5.3:13378586-13378605 MS.gene063670:CDS 35.0%
TCTCATTGAGAGCTAATACA+TGG + chr5.3:13378462-13378481 MS.gene063670:CDS 35.0%
! ATTTCTCAGTATATGGGCTA+TGG + chr5.3:13378715-13378734 MS.gene063670:CDS 35.0%
AACAGAAGATGGATGGAGAT+TGG + chr5.3:13378886-13378905 MS.gene063670:CDS 40.0%
AAGCTACAACTTGTGATGTG+TGG + chr5.3:13378681-13378700 MS.gene063670:CDS 40.0%
AATACGAAGTGCATTCGTCT+TGG + chr5.3:13378777-13378796 MS.gene063670:CDS 40.0%
ACTTGTGATGTGTGGGTATT+TGG + chr5.3:13378689-13378708 MS.gene063670:CDS 40.0%
AGCTACAACTTGTGATGTGT+GGG + chr5.3:13378682-13378701 MS.gene063670:CDS 40.0%
AGGGTAATCAGTAGAGGAAT+TGG - chr5.3:13378519-13378538 None:intergenic 40.0%
TCATTGGAACAGAAGATGGA+TGG + chr5.3:13378879-13378898 MS.gene063670:CDS 40.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 13378365 13378943 13378365 ID=MS.gene063670
chr5.3 mRNA 13378365 13378943 13378365 ID=MS.gene063670.t1;Parent=MS.gene063670
chr5.3 exon 13378365 13378943 13378365 ID=MS.gene063670.t1.exon1;Parent=MS.gene063670.t1
chr5.3 CDS 13378365 13378943 13378365 ID=cds.MS.gene063670.t1;Parent=MS.gene063670.t1
Gene Sequence

>MS.gene063670

ATGGTTTTGGGTACGTACGAGGAGTCAACAGATGATTACTTCTTGGTCACAATTTCTCAAGACCCCAAATTAGCTAATACTAAGCATTTGGAGATTTTCTCATTGAGAGCTAATACATGGAAAGAAATTGAGGGTATTCACTGCCCTTATACCAATTCCTCTACTGATTACCCTAAAATAATAGGGTCTCTTTTTAATGGGGCTATTCATTGGTTCGCTTTTCGTCATGATCATTTACGAAAGGATCTTATTCTTGCCTTTGACTTAATCAAAAGGGAACTTTTTGAGATCCCTCTCCCATTTGATATTGACTATGAAGCTACAACTTGTGATGTGTGGGTATTTGGAGAATTTCTCAGTATATGGGCTATGGATTATGCTAATGATACAGTTGAAATATGGGTGATGAAAGAATACGAAGTGCATTCGTCTTGGACGAAAACTCTTGTTTTGTCTATTGACGGCATTTCCACTAAGCTATTTTCCCCTATATGTTCTACAAAAAGTGGTGATATCATTGGAACAGAAGATGGATGGAGATTGGTGAAGTATAATGACAAAGGACAGTTGATAGAGTGA

Protein sequence

>MS.gene063670.t1

MVLGTYEESTDDYFLVTISQDPKLANTKHLEIFSLRANTWKEIEGIHCPYTNSSTDYPKIIGSLFNGAIHWFAFRHDHLRKDLILAFDLIKRELFEIPLPFDIDYEATTCDVWVFGEFLSIWAMDYANDTVEIWVMKEYEVHSSWTKTLVLSIDGISTKLFSPICSTKSGDIIGTEDGWRLVKYNDKGQLIE