AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033444

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033444.t1 MTR_8g102520 76.959 217 48 2 1 215 106 322 2.86e-114 329
MS.gene033444.t1 MTR_7g079400 62.896 221 76 3 1 215 117 337 5.69e-91 270
MS.gene033444.t1 MTR_8g098830 60.000 195 70 4 23 215 33 221 2.94e-76 229
MS.gene033444.t1 MTR_7g079370 56.019 216 91 4 2 213 139 354 1.22e-71 222
MS.gene033444.t1 MTR_7g079450 74.820 139 32 1 77 215 2 137 1.25e-69 209
MS.gene033444.t1 MTR_7g079420 58.791 182 63 5 42 215 967 1144 6.24e-57 194
MS.gene033444.t1 MTR_2g048580 50.463 216 84 8 10 215 140 342 1.28e-56 183
MS.gene033444.t1 MTR_4g117720 48.848 217 99 7 2 211 162 373 1.00e-55 181
MS.gene033444.t1 MTR_6g060650 47.706 218 100 8 2 214 164 372 6.92e-52 171
MS.gene033444.t1 MTR_1g055195 42.081 221 110 6 2 215 269 478 1.12e-44 155
MS.gene033444.t1 MTR_1g055175 44.444 198 106 4 21 215 5 201 1.62e-44 147
MS.gene033444.t1 MTR_5g097210 42.925 212 115 5 2 210 150 358 2.15e-44 152
MS.gene033444.t1 MTR_5g070130 42.922 219 114 6 2 211 156 372 1.86e-43 150
MS.gene033444.t1 MTR_3g103020 43.836 219 112 7 3 215 165 378 2.62e-43 149
MS.gene033444.t1 MTR_4g116320 42.180 211 114 6 3 209 46 252 9.71e-43 145
MS.gene033444.t1 MTR_4g109280 45.128 195 98 7 25 215 170 359 1.30e-42 147
MS.gene033444.t1 MTR_5g070088 40.930 215 119 5 2 211 170 381 1.89e-42 147
MS.gene033444.t1 MTR_4g118380 45.685 197 91 6 2 187 166 357 2.25e-41 144
MS.gene033444.t1 MTR_5g070080 43.182 220 112 9 2 215 174 386 6.65e-41 143
MS.gene033444.t1 MTR_4g023080 42.857 217 105 8 2 214 152 353 6.72e-40 140
MS.gene033444.t1 MTR_4g023130 41.475 217 108 7 2 214 111 312 7.67e-40 139
MS.gene033444.t1 MTR_4g116330 43.379 219 106 8 5 215 172 380 8.20e-40 140
MS.gene033444.t1 MTR_1g055135 42.601 223 116 6 2 215 156 375 1.71e-39 139
MS.gene033444.t1 MTR_4g118400 43.850 187 86 6 25 209 125 294 2.92e-39 137
MS.gene033444.t1 MTR_3g034180 42.857 231 102 11 2 214 270 488 3.53e-39 140
MS.gene033444.t1 MTR_6g463430 42.289 201 106 7 18 215 9 202 8.33e-39 133
MS.gene033444.t1 MTR_4g094365 42.273 220 113 9 1 215 154 364 1.43e-38 137
MS.gene033444.t1 MTR_8g433030 43.868 212 107 9 5 211 134 338 2.85e-38 135
MS.gene033444.t1 MTR_4g122850 41.096 219 120 9 2 215 150 364 3.10e-38 136
MS.gene033444.t1 MTR_4g117850 41.667 216 115 7 3 210 163 375 4.98e-38 135
MS.gene033444.t1 MTR_8g032390 43.868 212 107 9 5 211 167 371 5.42e-38 135
MS.gene033444.t1 MTR_4g109270 43.119 218 108 8 3 215 158 364 1.03e-37 134
MS.gene033444.t1 MTR_3g034130 40.887 203 96 7 2 188 156 350 1.22e-37 134
MS.gene033444.t1 MTR_1g055250 41.919 198 109 5 2 196 56 250 1.37e-37 133
MS.gene033444.t1 MTR_8g040935 40.909 198 107 7 25 215 14 208 3.06e-37 129
MS.gene033444.t1 MTR_3g034160 41.778 225 107 8 5 214 159 374 3.28e-37 133
MS.gene033444.t1 MTR_4g117750 40.724 221 122 5 2 214 139 358 3.72e-37 135
MS.gene033444.t1 MTR_4g117750 37.410 139 70 6 86 209 341 477 3.60e-15 73.9
MS.gene033444.t1 MTR_4g117770 41.818 220 112 7 3 215 157 367 5.05e-36 130
MS.gene033444.t1 MTR_4g117810 41.818 220 112 7 3 215 157 367 5.05e-36 130
MS.gene033444.t1 MTR_4g021785 39.450 218 114 7 2 215 152 355 1.61e-35 128
MS.gene033444.t1 MTR_4g118370 40.566 212 103 6 2 210 64 255 4.39e-35 125
MS.gene033444.t1 MTR_1g055165 44.785 163 87 3 55 215 157 318 4.87e-35 126
MS.gene033444.t1 MTR_4g117780 39.269 219 119 7 3 215 157 367 6.39e-33 122
MS.gene033444.t1 MTR_4g023070 37.069 232 121 7 1 214 145 369 1.16e-32 121
MS.gene033444.t1 MTR_8g433040 40.092 217 112 6 2 215 163 364 3.47e-32 120
MS.gene033444.t1 MTR_8g032380 40.092 217 112 6 2 215 163 364 3.47e-32 120
MS.gene033444.t1 MTR_4g117530 38.462 195 82 6 23 215 95 253 4.60e-32 117
MS.gene033444.t1 MTR_4g023120 36.910 233 122 8 1 215 145 370 6.90e-31 116
MS.gene033444.t1 MTR_4g023090 36.937 222 113 9 5 209 167 378 5.80e-30 114
MS.gene033444.t1 MTR_4g023190 36.364 220 118 8 1 213 153 357 1.48e-28 110
MS.gene033444.t1 MTR_4g118360 48.333 120 60 2 92 209 1 120 5.47e-28 102
MS.gene033444.t1 MTR_4g023380 36.190 210 102 6 25 209 169 371 2.66e-27 107
MS.gene033444.t1 MTR_4g023150 37.755 196 108 8 25 213 170 358 4.49e-27 106
MS.gene033444.t1 MTR_4g023200 37.436 195 108 8 26 213 171 358 1.62e-26 104
MS.gene033444.t1 MTR_4g023440 34.562 217 94 7 1 215 162 332 4.51e-26 103
MS.gene033444.t1 MTR_4g117870 35.780 218 94 9 2 215 92 267 5.86e-26 101
MS.gene033444.t1 MTR_4g116330 37.273 220 79 9 5 215 172 341 7.17e-25 100
MS.gene033444.t1 MTR_4g114700 34.951 206 82 5 5 206 61 218 7.05e-22 90.1
MS.gene033444.t1 MTR_5g023000 47.872 94 46 2 124 215 7 99 1.81e-21 85.5
MS.gene033444.t1 MTR_4g117430 44.898 98 43 3 2 99 49 135 1.54e-19 82.0
MS.gene033444.t1 MTR_4g122860 33.846 195 91 8 29 215 156 320 7.51e-19 83.6
MS.gene033444.t1 MTR_7g117720 44.348 115 54 5 104 210 160 272 1.21e-17 79.3
MS.gene033444.t1 MTR_4g121580 32.547 212 107 10 2 207 164 345 2.56e-17 79.7
MS.gene033444.t1 MTR_8g013010 44.828 116 55 5 2 117 203 309 7.53e-17 77.8
MS.gene033444.t1 MTR_6g034720 42.718 103 55 3 1 102 79 178 2.17e-12 64.3
MS.gene033444.t1 MTR_0267s0050 30.075 133 74 4 57 170 214 346 4.63e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATTGCCTTTGATTTAA+AGG 0.116438 7.2:-25977073 MS.gene033444:CDS
TTGATTCTCATTTCCCTTAT+AGG 0.126352 7.2:-25977188 MS.gene033444:CDS
ACTCTGTATACACTGCTATT+TGG 0.172257 7.2:+25976719 None:intergenic
CAGTGATTACATGGTAGTTT+TGG 0.247840 7.2:-25977298 MS.gene033444:CDS
GGCAATAATAACATCCCTTT+TGG 0.284778 7.2:+25977086 None:intergenic
TCAGAGCTTGAAGTCCTATA+AGG 0.287235 7.2:+25977174 None:intergenic
CTCTGTATACACTGCTATTT+GGG 0.303953 7.2:+25976720 None:intergenic
TCTTGAATGGTTCTATTCAT+TGG 0.308230 7.2:-25977128 MS.gene033444:CDS
CCACCATTTGTAAAGCATAC+TGG 0.341955 7.2:+25976844 None:intergenic
AGATGTCTACACTTAAGATA+TGG 0.412369 7.2:-25976945 MS.gene033444:CDS
TAAGCTTGTTCAGTGCCATC+AGG 0.417768 7.2:+25976682 None:intergenic
AATGTCGGGTCGTTCTTGAA+TGG 0.418865 7.2:-25977141 MS.gene033444:CDS
TTTATAATTATGAGACCAAA+AGG 0.423323 7.2:-25977101 MS.gene033444:CDS
CATAGGAGTGATACTCTAAC+AGG 0.423667 7.2:+25976764 None:intergenic
CAGAGCTTGAAGTCCTATAA+GGG 0.435057 7.2:+25977175 None:intergenic
TTCGCGGACTTATCAGTGTA+TGG 0.439782 7.2:-25976975 MS.gene033444:CDS
ATTACTGTAATTGTATTTGA+AGG 0.443950 7.2:+25977272 None:intergenic
AATACAAAGTGCATTCATCT+TGG 0.445198 7.2:-25976912 MS.gene033444:CDS
ACTGATAAGTCCGCGAAATA+CGG 0.447963 7.2:+25976981 None:intergenic
TTCAAGAACGACCCGACATT+GGG 0.457176 7.2:+25977144 None:intergenic
TGTCATTTCCTTTAAATCAA+AGG 0.465778 7.2:+25977065 None:intergenic
ACAAATGGTGGTGATATTGT+TGG 0.467745 7.2:-25976832 MS.gene033444:CDS
ATCCCAGTATGCTTTACAAA+TGG 0.468448 7.2:-25976847 MS.gene033444:CDS
CATGTCAAGCAGTGATTACA+TGG 0.474278 7.2:-25977307 MS.gene033444:CDS
CACCATTTGTAAAGCATACT+GGG 0.484537 7.2:+25976845 None:intergenic
GCTAATCCACCATCAGCAAC+TGG 0.487853 7.2:+25976808 None:intergenic
CTGTTAGAGTATCACTCCTA+TGG 0.509163 7.2:-25976763 MS.gene033444:CDS
GATGTCTACACTTAAGATAT+GGG 0.517647 7.2:-25976944 MS.gene033444:CDS
TATGATTTGTCCGTATTTCG+CGG 0.518178 7.2:-25976991 MS.gene033444:CDS
TAATACTAGCTTGATTGACT+TGG 0.526898 7.2:-25977253 MS.gene033444:CDS
TCTTTGCTTTCACTCCCTGA+TGG 0.527790 7.2:-25976697 MS.gene033444:CDS
CTGAAGATGATCCCAATGTC+GGG 0.534343 7.2:-25977155 MS.gene033444:CDS
AAGCATACTGGGATAAAATC+CGG 0.535099 7.2:+25976856 None:intergenic
ATTCAAGAACGACCCGACAT+TGG 0.540647 7.2:+25977143 None:intergenic
CCAGTATGCTTTACAAATGG+TGG 0.556297 7.2:-25976844 MS.gene033444:CDS
CTCATTGAGAGCTAATGAGT+GGG 0.557112 7.2:-25977223 MS.gene033444:CDS
GTTGGACCAGTTGCTGATGG+TGG 0.559934 7.2:-25976814 MS.gene033444:CDS
ATTGTTGGACCAGTTGCTGA+TGG 0.574060 7.2:-25976817 MS.gene033444:CDS
ACTGGGATAAAATCCGGAGC+AGG 0.586298 7.2:+25976862 None:intergenic
TCTGAAGATGATCCCAATGT+CGG 0.596330 7.2:-25977156 MS.gene033444:CDS
TCTCATTGAGAGCTAATGAG+TGG 0.598081 7.2:-25977224 MS.gene033444:CDS
TTATAATTATGAGACCAAAA+GGG 0.608278 7.2:-25977100 MS.gene033444:CDS
ACTTATCAGTGTATGGAATG+TGG 0.619206 7.2:-25976968 MS.gene033444:CDS
AAGCTTGTTCAGTGCCATCA+GGG 0.620091 7.2:+25976683 None:intergenic
AAATTGATATTAAAATCATG+TGG 0.650461 7.2:+25977024 None:intergenic
TGCAGAGTAACAGTCACCAT+AGG 0.670737 7.2:+25976747 None:intergenic
TTAGCAAAGCTTGATAACAG+AGG 0.695669 7.2:-25976790 MS.gene033444:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGATATTAAAATCATG+TGG + chr7.2:25976966-25976985 None:intergenic 15.0%
!! ATTACTGTAATTGTATTTGA+AGG + chr7.2:25976718-25976737 None:intergenic 20.0%
!! TTATAATTATGAGACCAAAA+GGG - chr7.2:25976887-25976906 MS.gene033444:CDS 20.0%
!! TTTATAATTATGAGACCAAA+AGG - chr7.2:25976886-25976905 MS.gene033444:CDS 20.0%
!!! TATTATTGCCTTTGATTTAA+AGG - chr7.2:25976914-25976933 MS.gene033444:CDS 20.0%
!! TGTCATTTCCTTTAAATCAA+AGG + chr7.2:25976925-25976944 None:intergenic 25.0%
AATACAAAGTGCATTCATCT+TGG - chr7.2:25977075-25977094 MS.gene033444:CDS 30.0%
AGATGTCTACACTTAAGATA+TGG - chr7.2:25977042-25977061 MS.gene033444:CDS 30.0%
GATGTCTACACTTAAGATAT+GGG - chr7.2:25977043-25977062 MS.gene033444:CDS 30.0%
TTGATTCTCATTTCCCTTAT+AGG - chr7.2:25976799-25976818 MS.gene033444:CDS 30.0%
! AGTGATTACATGGTAGTTTT+GGG - chr7.2:25976690-25976709 MS.gene033444:CDS 30.0%
! TAATACTAGCTTGATTGACT+TGG - chr7.2:25976734-25976753 MS.gene033444:CDS 30.0%
! TCTTGAATGGTTCTATTCAT+TGG - chr7.2:25976859-25976878 MS.gene033444:CDS 30.0%
AAGCATACTGGGATAAAATC+CGG + chr7.2:25977134-25977153 None:intergenic 35.0%
ACTCTGTATACACTGCTATT+TGG + chr7.2:25977271-25977290 None:intergenic 35.0%
ACTTATCAGTGTATGGAATG+TGG - chr7.2:25977019-25977038 MS.gene033444:CDS 35.0%
ATCCCAGTATGCTTTACAAA+TGG - chr7.2:25977140-25977159 MS.gene033444:CDS 35.0%
CACCATTTGTAAAGCATACT+GGG + chr7.2:25977145-25977164 None:intergenic 35.0%
! ACAAATGGTGGTGATATTGT+TGG - chr7.2:25977155-25977174 MS.gene033444:CDS 35.0%
! CAGTGATTACATGGTAGTTT+TGG - chr7.2:25976689-25976708 MS.gene033444:CDS 35.0%
! CTCTGTATACACTGCTATTT+GGG + chr7.2:25977270-25977289 None:intergenic 35.0%
! GGCAATAATAACATCCCTTT+TGG + chr7.2:25976904-25976923 None:intergenic 35.0%
! TATGATTTGTCCGTATTTCG+CGG - chr7.2:25976996-25977015 MS.gene033444:CDS 35.0%
! TTAGCAAAGCTTGATAACAG+AGG - chr7.2:25977197-25977216 MS.gene033444:CDS 35.0%
! TTTTTCTTTTCATCCTGCTC+CGG - chr7.2:25977112-25977131 MS.gene033444:CDS 35.0%
!! GTGATTACATGGTAGTTTTG+GGG - chr7.2:25976691-25976710 MS.gene033444:CDS 35.0%
ACTGATAAGTCCGCGAAATA+CGG + chr7.2:25977009-25977028 None:intergenic 40.0%
CAGAGCTTGAAGTCCTATAA+GGG + chr7.2:25976815-25976834 None:intergenic 40.0%
CATAGGAGTGATACTCTAAC+AGG + chr7.2:25977226-25977245 None:intergenic 40.0%
CCACCATTTGTAAAGCATAC+TGG + chr7.2:25977146-25977165 None:intergenic 40.0%
CCAGTATGCTTTACAAATGG+TGG - chr7.2:25977143-25977162 MS.gene033444:CDS 40.0%
CTCATTGAGAGCTAATGAGT+GGG - chr7.2:25976764-25976783 MS.gene033444:CDS 40.0%
TCAGAGCTTGAAGTCCTATA+AGG + chr7.2:25976816-25976835 None:intergenic 40.0%
TCTCATTGAGAGCTAATGAG+TGG - chr7.2:25976763-25976782 MS.gene033444:CDS 40.0%
TCTGAAGATGATCCCAATGT+CGG - chr7.2:25976831-25976850 MS.gene033444:CDS 40.0%
! CTGTTAGAGTATCACTCCTA+TGG - chr7.2:25977224-25977243 MS.gene033444:CDS 40.0%
AATGTCGGGTCGTTCTTGAA+TGG - chr7.2:25976846-25976865 MS.gene033444:CDS 45.0%
ATTCAAGAACGACCCGACAT+TGG + chr7.2:25976847-25976866 None:intergenic 45.0%
ATTGTTGGACCAGTTGCTGA+TGG - chr7.2:25977170-25977189 MS.gene033444:CDS 45.0%
CTGAAGATGATCCCAATGTC+GGG - chr7.2:25976832-25976851 MS.gene033444:CDS 45.0%
TCTTTGCTTTCACTCCCTGA+TGG - chr7.2:25977290-25977309 MS.gene033444:CDS 45.0%
TGCAGAGTAACAGTCACCAT+AGG + chr7.2:25977243-25977262 None:intergenic 45.0%
TTCAAGAACGACCCGACATT+GGG + chr7.2:25976846-25976865 None:intergenic 45.0%
TTCGCGGACTTATCAGTGTA+TGG - chr7.2:25977012-25977031 MS.gene033444:CDS 45.0%
! AAGCTTGTTCAGTGCCATCA+GGG + chr7.2:25977307-25977326 None:intergenic 45.0%
! TAAGCTTGTTCAGTGCCATC+AGG + chr7.2:25977308-25977327 None:intergenic 45.0%
ACTGGGATAAAATCCGGAGC+AGG + chr7.2:25977128-25977147 None:intergenic 50.0%
GCTAATCCACCATCAGCAAC+TGG + chr7.2:25977182-25977201 None:intergenic 50.0%
GTTGGACCAGTTGCTGATGG+TGG - chr7.2:25977173-25977192 MS.gene033444:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 25976681 25977328 25976681 ID=MS.gene033444
chr7.2 mRNA 25976681 25977328 25976681 ID=MS.gene033444.t1;Parent=MS.gene033444
chr7.2 exon 25976681 25977328 25976681 ID=MS.gene033444.t1.exon1;Parent=MS.gene033444.t1
chr7.2 CDS 25976681 25977328 25976681 ID=cds.MS.gene033444.t1;Parent=MS.gene033444.t1
Gene Sequence

>MS.gene033444

ATGTCAAGCAGTGATTACATGGTAGTTTTGGGGTCCTTCAAATACAATTACAGTAATACTAGCTTGATTGACTTGGAGATTTTCTCATTGAGAGCTAATGAGTGGGAAAAAATTAAGTTTGATTCTCATTTCCCTTATAGGACTTCAAGCTCTGAAGATGATCCCAATGTCGGGTCGTTCTTGAATGGTTCTATTCATTGGCTGCTTTATAATTATGAGACCAAAAGGGATGTTATTATTGCCTTTGATTTAAAGGAAATGACAATGTTTGAGATTGCTCTTCCACATGATTTTAATATCAATTTTTCTAACGTTTATGATTTGTCCGTATTTCGCGGACTTATCAGTGTATGGAATGTGGAGATGTCTACACTTAAGATATGGGTAATGCAAGAATACAAAGTGCATTCATCTTGGACTAAGACTCTTGATTTTTCTTTTCATCCTGCTCCGGATTTTATCCCAGTATGCTTTACAAATGGTGGTGATATTGTTGGACCAGTTGCTGATGGTGGATTAGCAAAGCTTGATAACAGAGGAGACCTGTTAGAGTATCACTCCTATGGTGACTGTTACTCTGCAATATCCCAAATAGCAGTGTATACAGAGTCTTTGCTTTCACTCCCTGATGGCACTGAACAAGCTTAA
Protein sequence

>MS.gene033444.t1

MSSSDYMVVLGSFKYNYSNTSLIDLEIFSLRANEWEKIKFDSHFPYRTSSSEDDPNVGSFLNGSIHWLLYNYETKRDVIIAFDLKEMTMFEIALPHDFNINFSNVYDLSVFRGLISVWNVEMSTLKIWVMQEYKVHSSWTKTLDFSFHPAPDFIPVCFTNGGDIVGPVADGGLAKLDNRGDLLEYHSYGDCYSAISQIAVYTESLLSLPDGTEQA