AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35068


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35068.t1 MTR_4g094365 79.851 134 25 1 1 132 231 364 5.79e-70 214
MS.gene35068.t1 MTR_8g433040 61.364 132 49 1 1 132 235 364 1.58e-47 156
MS.gene35068.t1 MTR_8g032380 61.364 132 49 1 1 132 235 364 1.58e-47 156
MS.gene35068.t1 MTR_8g433030 62.992 127 44 2 1 126 212 336 4.50e-46 152
MS.gene35068.t1 MTR_8g032390 62.992 127 44 2 1 126 245 369 1.30e-45 152
MS.gene35068.t1 MTR_4g122850 58.209 134 53 3 1 132 232 364 1.68e-45 152
MS.gene35068.t1 MTR_4g116330 55.556 135 57 2 1 132 246 380 2.98e-43 146
MS.gene35068.t1 MTR_6g060650 56.061 132 55 2 1 131 243 372 1.31e-42 144
MS.gene35068.t1 MTR_3g103020 55.639 133 58 1 1 132 246 378 6.83e-42 142
MS.gene35068.t1 MTR_4g109280 57.778 135 51 4 1 132 228 359 1.07e-41 141
MS.gene35068.t1 MTR_8g040935 54.478 134 58 2 2 132 75 208 1.72e-41 137
MS.gene35068.t1 MTR_4g109270 55.639 133 58 1 1 132 232 364 8.14e-41 139
MS.gene35068.t1 MTR_4g116320 54.962 131 56 2 1 129 126 255 1.92e-40 135
MS.gene35068.t1 MTR_4g117780 58.036 112 46 1 22 132 256 367 4.53e-36 127
MS.gene35068.t1 MTR_4g117870 51.562 128 61 1 5 132 141 267 4.88e-36 124
MS.gene35068.t1 MTR_4g117770 58.036 112 46 1 22 132 256 367 5.25e-36 127
MS.gene35068.t1 MTR_4g117810 58.036 112 46 1 22 132 256 367 5.25e-36 127
MS.gene35068.t1 MTR_4g023130 54.135 133 55 4 1 131 184 312 9.50e-36 125
MS.gene35068.t1 MTR_3g034180 53.383 133 58 3 1 132 360 489 9.87e-36 128
MS.gene35068.t1 MTR_4g117720 52.273 132 55 4 1 126 242 371 1.11e-35 126
MS.gene35068.t1 MTR_4g117850 53.968 126 56 2 2 125 248 373 7.36e-35 124
MS.gene35068.t1 MTR_4g023080 52.632 133 57 4 1 131 225 353 2.65e-34 122
MS.gene35068.t1 MTR_4g118360 51.163 129 54 5 8 131 1 125 7.18e-34 115
MS.gene35068.t1 MTR_4g023380 45.860 157 58 5 1 132 225 379 1.13e-33 120
MS.gene35068.t1 MTR_4g122860 52.344 128 53 3 10 132 196 320 1.68e-33 119
MS.gene35068.t1 MTR_5g097210 52.344 128 59 1 2 129 235 360 6.13e-33 119
MS.gene35068.t1 MTR_4g023090 47.183 142 56 5 1 126 240 378 7.92e-33 119
MS.gene35068.t1 MTR_1g055175 46.970 132 68 2 1 131 70 200 1.02e-32 114
MS.gene35068.t1 MTR_5g023000 55.556 99 43 1 35 132 1 99 1.59e-32 110
MS.gene35068.t1 MTR_4g118370 50.769 130 59 3 1 129 132 257 2.59e-32 114
MS.gene35068.t1 MTR_4g117750 55.556 108 47 1 24 130 374 481 1.54e-31 116
MS.gene35068.t1 MTR_4g117750 46.281 121 60 3 12 131 242 358 2.11e-24 97.1
MS.gene35068.t1 MTR_4g116330 54.955 111 47 2 25 132 231 341 2.46e-31 114
MS.gene35068.t1 MTR_1g055165 46.970 132 68 2 1 131 187 317 2.71e-31 113
MS.gene35068.t1 MTR_4g021785 48.529 136 60 4 1 132 226 355 3.85e-31 114
MS.gene35068.t1 MTR_4g023070 46.528 144 61 4 2 131 228 369 4.28e-31 114
MS.gene35068.t1 MTR_1g055195 50.781 128 58 3 1 126 348 472 4.96e-31 115
MS.gene35068.t1 MTR_6g463430 50.000 134 60 4 1 132 74 202 9.59e-31 109
MS.gene35068.t1 MTR_3g034160 53.788 132 57 3 1 131 246 374 1.71e-30 112
MS.gene35068.t1 MTR_4g023120 45.270 148 59 4 2 132 228 370 6.06e-30 110
MS.gene35068.t1 MTR_3g034130 52.252 111 50 2 1 110 246 354 1.19e-29 110
MS.gene35068.t1 MTR_1g055135 47.059 136 65 4 1 131 241 374 7.11e-29 108
MS.gene35068.t1 MTR_4g023190 46.715 137 62 6 1 130 225 357 1.64e-28 107
MS.gene35068.t1 MTR_5g070080 47.619 126 64 2 2 126 255 379 2.53e-28 107
MS.gene35068.t1 MTR_4g023200 46.715 137 62 6 1 130 226 358 3.49e-28 106
MS.gene35068.t1 MTR_5g070130 46.094 128 67 2 1 126 243 370 8.23e-28 105
MS.gene35068.t1 MTR_4g023150 45.985 137 63 6 1 130 226 358 3.82e-27 103
MS.gene35068.t1 MTR_4g118380 47.826 115 48 3 1 105 245 357 5.31e-27 102
MS.gene35068.t1 MTR_4g117530 51.376 109 47 3 25 132 150 253 2.49e-26 99.4
MS.gene35068.t1 MTR_7g079450 45.455 132 66 4 2 132 11 137 4.92e-26 95.5
MS.gene35068.t1 MTR_4g118400 45.238 126 54 3 2 126 183 294 1.23e-25 98.2
MS.gene35068.t1 MTR_8g102520 45.522 134 67 4 2 132 192 322 2.87e-25 97.8
MS.gene35068.t1 MTR_4g023440 57.447 94 35 3 41 132 242 332 7.97e-25 96.7
MS.gene35068.t1 MTR_5g070088 42.969 128 71 2 1 126 252 379 1.23e-23 94.4
MS.gene35068.t1 MTR_1g055250 46.667 105 54 2 2 106 140 242 1.79e-23 93.2
MS.gene35068.t1 MTR_7g079400 42.754 138 69 5 2 132 203 337 4.45e-21 86.7
MS.gene35068.t1 MTR_4g114700 52.874 87 37 2 41 123 132 218 1.77e-19 81.3
MS.gene35068.t1 MTR_4g121580 40.458 131 63 5 4 131 230 348 2.50e-18 79.7
MS.gene35068.t1 MTR_7g117720 54.651 86 34 3 46 126 186 271 3.35e-18 78.2
MS.gene35068.t1 MTR_4g122840 38.462 130 45 3 4 132 169 264 5.09e-18 77.8
MS.gene35068.t1 MTR_8g013005 62.712 59 22 0 74 132 1 59 1.58e-17 71.6
MS.gene35068.t1 MTR_4g023430 53.659 82 32 3 56 132 142 222 8.47e-17 73.6
MS.gene35068.t1 MTR_7g079370 41.085 129 67 5 2 126 227 350 3.43e-16 73.6
MS.gene35068.t1 MTR_8g098830 43.396 106 54 4 2 105 97 198 5.70e-16 71.6
MS.gene35068.t1 MTR_7g079420 39.231 130 70 5 2 126 1013 1138 2.94e-15 71.2
MS.gene35068.t1 MTR_4g023100 57.895 57 22 1 76 132 65 119 3.82e-14 64.3
MS.gene35068.t1 MTR_4g109290 62.264 53 20 0 80 132 136 188 2.78e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTGGTTCGTGGGTATT+TGG 0.245938 4.4:+20383963 MS.gene35068:CDS
ATAGCCGTACACTTAAAATA+TGG 0.266688 4.4:+20384018 MS.gene35068:CDS
TAAACTTGCTCTCTATCAAC+AGG 0.362152 4.4:-20384281 None:intergenic
CACGATGGTCCTGCATATCT+TGG 0.377345 4.4:+20383948 MS.gene35068:CDS
ATTGGATCAGACGGTGGTCT+TGG 0.412575 4.4:+20384152 MS.gene35068:CDS
ACTGTAAACTGCATCCATCT+TGG 0.469118 4.4:+20384051 MS.gene35068:CDS
TCGTGATAATCATCTGGAAC+AGG 0.478738 4.4:-20383930 None:intergenic
TTGCCTTTAATTTAATGGAA+AGG 0.482467 4.4:+20383892 None:intergenic
GTCCTGCATATCTTGGTTCG+TGG 0.485547 4.4:+20383955 MS.gene35068:CDS
GGACCATCGTGATAATCATC+TGG 0.487786 4.4:-20383936 None:intergenic
TTGGTGAAGTATAATGATAA+AGG 0.492510 4.4:+20384176 MS.gene35068:CDS
TAGCCGTACACTTAAAATAT+GGG 0.498718 4.4:+20384019 MS.gene35068:CDS
CTCTGTATACACGATCGCTT+GGG 0.514295 4.4:-20384243 None:intergenic
ACTCTGTATACACGATCGCT+TGG 0.527545 4.4:-20384244 None:intergenic
ACAAGAGTCTTAGTCCAAGA+TGG 0.541795 4.4:-20384065 None:intergenic
ATCAGACGGTGGTCTTGGAT+TGG 0.544899 4.4:+20384157 MS.gene35068:CDS
ACCCACGAACCAAGATATGC+AGG 0.553874 4.4:-20383957 None:intergenic
TCCTGCATATCTTGGTTCGT+GGG 0.554694 4.4:+20383956 MS.gene35068:CDS
GGGACCTAAGTGGATAGTTA+CGG 0.559472 4.4:-20384223 None:intergenic
GGTGATATTATTGGATCAGA+CGG 0.605256 4.4:+20384143 MS.gene35068:CDS
TAGAGGGGAAAATATGCGAA+TGG 0.624574 4.4:-20384105 None:intergenic
CCTACCGTAACTATCCACTT+AGG 0.631176 4.4:+20384219 MS.gene35068:CDS
ACGATCGCTTGGGACCTAAG+TGG 0.637531 4.4:-20384233 None:intergenic
CCTAAGTGGATAGTTACGGT+AGG 0.679593 4.4:-20384219 None:intergenic
GTTCCAGATGATTATCACGA+TGG 0.682457 4.4:+20383933 MS.gene35068:CDS
GATATTATTGGATCAGACGG+TGG 0.700053 4.4:+20384146 MS.gene35068:CDS
AAACTTGCTCTCTATCAACA+GGG 0.707501 4.4:-20384280 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACAAAAAGTGGTGATATTAT+TGG + chr4.4:20384134-20384153 MS.gene35068:CDS 25.0%
! TTGGTGAAGTATAATGATAA+AGG + chr4.4:20384176-20384195 MS.gene35068:CDS 25.0%
ATAGCCGTACACTTAAAATA+TGG + chr4.4:20384018-20384037 MS.gene35068:CDS 30.0%
TAGCCGTACACTTAAAATAT+GGG + chr4.4:20384019-20384038 MS.gene35068:CDS 30.0%
! ACTTTTTGTAGAACATAGAG+GGG - chr4.4:20384123-20384142 None:intergenic 30.0%
!! CACTTTTTGTAGAACATAGA+GGG - chr4.4:20384124-20384143 None:intergenic 30.0%
!! TCACCCATATTTTAAGTGTA+CGG - chr4.4:20384025-20384044 None:intergenic 30.0%
!! TGGAGAATTTTTCAGTCTAT+GGG + chr4.4:20383983-20384002 MS.gene35068:CDS 30.0%
!! TTGGAGAATTTTTCAGTCTA+TGG + chr4.4:20383982-20384001 MS.gene35068:CDS 30.0%
CCTCTATGTTCTACAAAAAG+TGG + chr4.4:20384122-20384141 MS.gene35068:CDS 35.0%
GGTGATATTATTGGATCAGA+CGG + chr4.4:20384143-20384162 MS.gene35068:CDS 35.0%
! AAACTTGCTCTCTATCAACA+GGG - chr4.4:20384283-20384302 None:intergenic 35.0%
! TAAACTTGCTCTCTATCAAC+AGG - chr4.4:20384284-20384303 None:intergenic 35.0%
!! ATTTTTCAGTCTATGGGCTA+TGG + chr4.4:20383989-20384008 MS.gene35068:CDS 35.0%
!!! CCACTTTTTGTAGAACATAG+AGG - chr4.4:20384125-20384144 None:intergenic 35.0%
ACAAGAGTCTTAGTCCAAGA+TGG - chr4.4:20384068-20384087 None:intergenic 40.0%
ACTGTAAACTGCATCCATCT+TGG + chr4.4:20384051-20384070 MS.gene35068:CDS 40.0%
GATATTATTGGATCAGACGG+TGG + chr4.4:20384146-20384165 MS.gene35068:CDS 40.0%
GTTCCAGATGATTATCACGA+TGG + chr4.4:20383933-20383952 MS.gene35068:CDS 40.0%
TAGAGGGGAAAATATGCGAA+TGG - chr4.4:20384108-20384127 None:intergenic 40.0%
! TATCTTGGTTCGTGGGTATT+TGG + chr4.4:20383963-20383982 MS.gene35068:CDS 40.0%
! TCGTGATAATCATCTGGAAC+AGG - chr4.4:20383933-20383952 None:intergenic 40.0%
ACTCTGTATACACGATCGCT+TGG - chr4.4:20384247-20384266 None:intergenic 45.0%
CCTAAGTGGATAGTTACGGT+AGG - chr4.4:20384222-20384241 None:intergenic 45.0%
CCTACCGTAACTATCCACTT+AGG + chr4.4:20384219-20384238 MS.gene35068:CDS 45.0%
CTCTGTATACACGATCGCTT+GGG - chr4.4:20384246-20384265 None:intergenic 45.0%
GGACCATCGTGATAATCATC+TGG - chr4.4:20383939-20383958 None:intergenic 45.0%
GGGACCTAAGTGGATAGTTA+CGG - chr4.4:20384226-20384245 None:intergenic 45.0%
! TCCTGCATATCTTGGTTCGT+GGG + chr4.4:20383956-20383975 MS.gene35068:CDS 45.0%
ACCCACGAACCAAGATATGC+AGG - chr4.4:20383960-20383979 None:intergenic 50.0%
ATTGGATCAGACGGTGGTCT+TGG + chr4.4:20384152-20384171 MS.gene35068:CDS 50.0%
CACGATGGTCCTGCATATCT+TGG + chr4.4:20383948-20383967 MS.gene35068:CDS 50.0%
! ATCAGACGGTGGTCTTGGAT+TGG + chr4.4:20384157-20384176 MS.gene35068:CDS 50.0%
! GTCCTGCATATCTTGGTTCG+TGG + chr4.4:20383955-20383974 MS.gene35068:CDS 50.0%
ACGATCGCTTGGGACCTAAG+TGG - chr4.4:20384236-20384255 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 20383906 20384304 20383906 ID=MS.gene35068
chr4.4 mRNA 20383906 20384304 20383906 ID=MS.gene35068.t1;Parent=MS.gene35068
chr4.4 exon 20383906 20384304 20383906 ID=MS.gene35068.t1.exon1;Parent=MS.gene35068.t1
chr4.4 CDS 20383906 20384304 20383906 ID=cds.MS.gene35068.t1;Parent=MS.gene35068.t1
Gene Sequence

>MS.gene35068

ATGGAAAGGAAACTGTTAGAGATACCTGTTCCAGATGATTATCACGATGGTCCTGCATATCTTGGTTCGTGGGTATTTGGAGAATTTTTCAGTCTATGGGCTATGGATCATTATAGCCGTACACTTAAAATATGGGTGATGAATGACTGTAAACTGCATCCATCTTGGACTAAGACTCTTGTTGTTCGTATTGATGATGCCATTCGCATATTTTCCCCTCTATGTTCTACAAAAAGTGGTGATATTATTGGATCAGACGGTGGTCTTGGATTGGTGAAGTATAATGATAAAGGACAACTCTTAGAGTATTGCGCCTACCGTAACTATCCACTTAGGTCCCAAGCGATCGTGTATACAGAGTCTCTGCTTTCACTCCCTGTTGATAGAGAGCAAGTTTAA

Protein sequence

>MS.gene35068.t1

MERKLLEIPVPDDYHDGPAYLGSWVFGEFFSLWAMDHYSRTLKIWVMNDCKLHPSWTKTLVVRIDDAIRIFSPLCSTKSGDIIGSDGGLGLVKYNDKGQLLEYCAYRNYPLRSQAIVYTESLLSLPVDREQV