AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene036662


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036662.t1 MTR_4g116330 93.401 197 13 0 1 197 184 380 2.05e-134 381
MS.gene036662.t1 MTR_4g116330 74.619 197 11 1 1 197 184 341 3.62e-96 283
MS.gene036662.t1 MTR_4g116320 69.474 190 56 1 6 195 69 256 1.70e-90 266
MS.gene036662.t1 MTR_3g103020 63.077 195 67 2 6 197 186 378 2.09e-77 236
MS.gene036662.t1 MTR_8g040935 55.051 198 86 2 3 197 11 208 1.28e-70 213
MS.gene036662.t1 MTR_4g109280 58.549 193 76 3 6 197 170 359 4.05e-68 212
MS.gene036662.t1 MTR_6g060650 57.592 191 77 2 6 196 186 372 1.07e-67 211
MS.gene036662.t1 MTR_4g117850 59.067 193 73 4 6 194 186 376 1.48e-67 211
MS.gene036662.t1 MTR_4g117720 58.883 197 74 5 6 197 184 378 1.67e-67 211
MS.gene036662.t1 MTR_3g034180 56.566 198 74 5 6 196 296 488 7.65e-64 204
MS.gene036662.t1 MTR_4g094365 54.639 194 83 3 6 197 174 364 1.02e-62 198
MS.gene036662.t1 MTR_4g117780 53.061 196 86 3 6 197 174 367 4.08e-62 197
MS.gene036662.t1 MTR_4g023130 56.316 190 76 4 6 194 127 310 4.41e-62 195
MS.gene036662.t1 MTR_4g023080 55.263 190 78 4 6 194 168 351 1.23e-61 195
MS.gene036662.t1 MTR_3g034160 56.500 200 71 5 6 196 182 374 2.02e-61 195
MS.gene036662.t1 MTR_4g109270 54.872 195 82 3 6 197 173 364 2.07e-61 195
MS.gene036662.t1 MTR_4g023090 52.657 207 77 5 6 196 184 385 3.62e-61 195
MS.gene036662.t1 MTR_4g122850 53.465 202 79 8 4 197 170 364 7.38e-61 194
MS.gene036662.t1 MTR_4g021785 53.368 193 84 3 6 197 168 355 1.62e-59 190
MS.gene036662.t1 MTR_3g034130 53.968 189 75 4 6 184 182 368 1.07e-58 188
MS.gene036662.t1 MTR_4g117770 52.551 196 87 3 6 197 174 367 2.58e-58 187
MS.gene036662.t1 MTR_4g117810 52.551 196 87 3 6 197 174 367 2.58e-58 187
MS.gene036662.t1 MTR_4g118380 55.233 172 66 2 6 168 188 357 3.21e-58 187
MS.gene036662.t1 MTR_4g023380 50.463 216 78 6 6 197 169 379 3.00e-57 185
MS.gene036662.t1 MTR_6g463430 51.813 193 84 4 4 194 14 199 1.15e-56 178
MS.gene036662.t1 MTR_8g433030 53.158 190 81 5 6 192 153 337 4.05e-56 181
MS.gene036662.t1 MTR_4g023070 50.244 205 78 5 6 194 171 367 6.78e-56 181
MS.gene036662.t1 MTR_8g032390 53.158 190 81 5 6 192 186 370 1.22e-55 180
MS.gene036662.t1 MTR_4g023120 49.268 205 80 5 6 194 171 367 5.89e-53 173
MS.gene036662.t1 MTR_4g118400 47.872 188 78 4 6 191 125 294 7.24e-52 168
MS.gene036662.t1 MTR_5g097210 50.510 196 83 7 6 195 174 361 1.23e-49 165
MS.gene036662.t1 MTR_4g023200 52.356 191 80 6 9 194 173 357 2.37e-49 164
MS.gene036662.t1 MTR_1g055175 46.341 205 95 7 1 197 4 201 5.37e-49 158
MS.gene036662.t1 MTR_4g023190 52.062 194 81 6 6 194 170 356 7.85e-49 162
MS.gene036662.t1 MTR_8g433040 48.969 194 83 4 6 197 185 364 1.30e-48 162
MS.gene036662.t1 MTR_8g032380 48.969 194 83 4 6 197 185 364 1.30e-48 162
MS.gene036662.t1 MTR_4g023150 51.031 194 84 6 6 194 170 357 4.05e-48 160
MS.gene036662.t1 MTR_4g117870 47.917 192 62 4 6 197 114 267 5.80e-48 157
MS.gene036662.t1 MTR_5g070080 49.746 197 88 5 6 197 196 386 5.78e-46 155
MS.gene036662.t1 MTR_1g055135 47.264 201 86 8 9 197 183 375 1.64e-45 154
MS.gene036662.t1 MTR_4g023440 45.833 192 62 4 6 197 183 332 2.04e-45 153
MS.gene036662.t1 MTR_1g055195 45.755 212 89 8 1 197 278 478 1.38e-44 154
MS.gene036662.t1 MTR_4g118370 45.361 194 77 6 6 194 88 257 7.15e-43 144
MS.gene036662.t1 MTR_5g070130 46.040 202 97 5 6 197 178 377 7.96e-43 147
MS.gene036662.t1 MTR_4g117530 43.299 194 71 4 6 197 97 253 8.70e-43 144
MS.gene036662.t1 MTR_4g117750 47.236 199 88 6 6 193 163 355 5.24e-42 147
MS.gene036662.t1 MTR_4g117750 62.727 110 39 1 85 194 373 480 1.98e-36 132
MS.gene036662.t1 MTR_1g055165 48.214 168 76 5 34 197 158 318 8.83e-42 143
MS.gene036662.t1 MTR_5g070088 46.597 191 94 4 6 192 194 380 1.56e-41 144
MS.gene036662.t1 MTR_1g055250 47.826 184 83 7 6 184 78 253 7.48e-41 141
MS.gene036662.t1 MTR_4g118360 56.818 132 47 4 70 197 1 126 2.33e-39 131
MS.gene036662.t1 MTR_5g023000 65.000 100 33 1 98 197 2 99 1.74e-38 128
MS.gene036662.t1 MTR_4g122860 42.708 192 77 5 11 197 157 320 2.63e-37 132
MS.gene036662.t1 MTR_7g079370 43.590 195 100 6 2 191 161 350 1.14e-35 128
MS.gene036662.t1 MTR_7g079400 44.118 204 95 8 6 197 141 337 2.34e-35 127
MS.gene036662.t1 MTR_8g102520 42.500 200 100 7 6 197 130 322 3.99e-34 123
MS.gene036662.t1 MTR_4g121580 43.850 187 67 7 6 187 186 339 4.93e-33 122
MS.gene036662.t1 MTR_4g114700 39.785 186 61 4 6 188 81 218 1.89e-29 109
MS.gene036662.t1 MTR_8g098830 44.311 167 78 7 6 164 35 194 2.31e-29 108
MS.gene036662.t1 MTR_2g048580 36.715 207 94 10 6 197 158 342 7.86e-26 102
MS.gene036662.t1 MTR_8g013010 53.684 95 40 1 6 100 225 315 1.26e-24 98.6
MS.gene036662.t1 MTR_7g117720 53.704 108 45 3 90 192 165 272 7.39e-24 95.5
MS.gene036662.t1 MTR_7g079450 44.595 148 63 6 56 197 3 137 3.21e-23 90.5
MS.gene036662.t1 MTR_4g117430 51.087 92 42 2 6 96 65 154 4.36e-23 90.5
MS.gene036662.t1 MTR_7g079420 39.474 190 95 9 19 197 964 1144 1.18e-22 95.1
MS.gene036662.t1 MTR_4g122840 42.029 138 42 4 61 197 164 264 2.81e-22 91.3
MS.gene036662.t1 MTR_4g023430 55.556 81 35 1 118 197 142 222 9.59e-22 89.0
MS.gene036662.t1 MTR_8g013005 64.516 62 18 2 137 197 1 59 1.06e-20 81.6
MS.gene036662.t1 MTR_2g426020 29.208 202 129 7 4 196 189 385 4.68e-19 84.3
MS.gene036662.t1 MTR_4g109290 65.455 55 17 1 143 197 136 188 6.34e-15 70.1
MS.gene036662.t1 MTR_2g024110 28.638 213 115 11 6 194 171 370 8.09e-15 72.0
MS.gene036662.t1 MTR_4g023100 58.621 58 20 1 139 196 65 118 1.30e-14 67.4
MS.gene036662.t1 MTR_6g090180 26.042 192 131 4 3 190 250 434 7.91e-14 69.3
MS.gene036662.t1 MTR_6g034720 47.887 71 37 0 6 76 104 174 3.65e-13 65.9
MS.gene036662.t1 MTR_3g083010 28.019 207 133 6 4 196 198 402 5.01e-13 67.0
MS.gene036662.t1 MTR_2g025960 28.500 200 127 8 6 194 99 293 4.81e-12 63.9
MS.gene036662.t1 MTR_2g028130 26.699 206 132 8 4 194 193 394 9.96e-12 63.2
MS.gene036662.t1 MTR_4g116340 53.448 58 27 0 3 60 190 247 2.78e-11 61.2
MS.gene036662.t1 MTR_4g133170 29.677 155 98 7 6 155 174 322 4.52e-11 61.2
MS.gene036662.t1 MTR_8g101580 23.936 188 130 4 7 189 215 394 5.56e-11 60.8
MS.gene036662.t1 MTR_2g425980 28.500 200 124 9 7 194 191 383 6.47e-11 60.8
MS.gene036662.t1 MTR_8g099460 26.786 168 113 6 6 167 185 348 9.92e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036662.t1 AT3G06240 28.261 184 122 4 9 189 243 419 6.68e-14 69.7

Find 48 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAATTGCATTTGATTTAA+TGG 0.100322 8.1:+10682502 MS.gene036662:CDS
TTGGAGAATTTCTTAGTTTA+AGG 0.125843 8.1:+10682597 MS.gene036662:CDS
AATTGTAATTTGTGGGTATT+TGG 0.172259 8.1:+10682578 MS.gene036662:CDS
TGTCATTATACTTCAGTAAT+TGG 0.202744 8.1:-10682790 None:intergenic
TGTAGAGCATATTGGGTAAA+AGG 0.229942 8.1:-10682732 None:intergenic
TTCTAGAGCATGTATCATTC+TGG 0.290134 8.1:+10682822 MS.gene036662:CDS
ACTCTTGTTCTTCCTATTGA+TGG 0.297614 8.1:+10682695 MS.gene036662:CDS
TCAGAAGCATAGAGAATATA+CGG 0.346486 8.1:-10682407 None:intergenic
TGGAGAATTTCTTAGTTTAA+GGG 0.357415 8.1:+10682598 MS.gene036662:CDS
CTAATGAAAGAGTTGAAATA+TGG 0.360862 8.1:+10682633 MS.gene036662:CDS
TATGCTTCTGATAACGTAGT+AGG 0.362895 8.1:+10682419 MS.gene036662:CDS
AACTACTAATTGTAATTTGT+GGG 0.393998 8.1:+10682571 MS.gene036662:CDS
TGGGACGGTCCACTTCCATT+TGG 0.399776 8.1:+10682842 MS.gene036662:CDS
GAGTCTTTGCTTTCACTCCC+TGG 0.417958 8.1:+10682887 MS.gene036662:CDS
CAACTACTAATTGTAATTTG+TGG 0.431415 8.1:+10682570 MS.gene036662:CDS
TTATGGAGCTATTCATTGGT+TGG 0.431689 8.1:+10682451 MS.gene036662:CDS
TAGTTTAAGGGCCAACGATT+GGG 0.435448 8.1:+10682610 MS.gene036662:CDS
TTTCATTGAGAGATAATACT+TGG 0.452810 8.1:+10682360 MS.gene036662:CDS
TAATGGGCTATCCGTGTTTG+AGG 0.456060 8.1:+10682333 None:intergenic
AATTACAATTAGTAGTTGAA+TGG 0.460653 8.1:-10682565 None:intergenic
ACTCTGTATACACGGTCACT+TGG 0.479905 8.1:-10682868 None:intergenic
GGAGTCGAAATGCCATCAAT+AGG 0.481597 8.1:-10682707 None:intergenic
TTAGTTTAAGGGCCAACGAT+TGG 0.487546 8.1:+10682609 MS.gene036662:CDS
TCTAGAGCATGTATCATTCT+GGG 0.499103 8.1:+10682823 MS.gene036662:CDS
GTTGAATGGTGAAAACCATC+AGG 0.508102 8.1:-10682551 None:intergenic
TGGTGAAAACCATCAGGAAA+AGG 0.509773 8.1:-10682545 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.518219 8.1:+10682666 MS.gene036662:CDS
TAGGCTTGCACATTGTCACC+AGG 0.522054 8.1:-10682905 None:intergenic
ACGGTCACTTGGGATCCAAA+TGG 0.522598 8.1:-10682857 None:intergenic
AGAGCATATTGGGTAAAAGG+AGG 0.523892 8.1:-10682729 None:intergenic
TTGTTCGTGGTGATATAAAG+AGG 0.527772 8.1:+10682474 MS.gene036662:CDS
ATTCATTGGTTGGTTGTTCG+TGG 0.535905 8.1:+10682461 MS.gene036662:CDS
TAATGAAAGAGTTGAAATAT+GGG 0.539316 8.1:+10682634 MS.gene036662:CDS
ACTTGGGATCCAAATGGAAG+TGG 0.547144 8.1:-10682851 None:intergenic
CTCTGTATACACGGTCACTT+GGG 0.553016 8.1:-10682867 None:intergenic
CAGAAGCATAGAGAATATAC+GGG 0.554093 8.1:-10682406 None:intergenic
ATGCTTCTGATAACGTAGTA+GGG 0.561315 8.1:+10682420 MS.gene036662:CDS
TTGCATTTGATTTAATGGAT+CGG 0.568157 8.1:+10682507 MS.gene036662:CDS
GAGCATATTGGGTAAAAGGA+GGG 0.572126 8.1:-10682728 None:intergenic
TCTTTCATTAGCCCAATCGT+TGG 0.591565 8.1:-10682621 None:intergenic
TAGAGAATATACGGGGAGTG+AGG 0.603876 8.1:-10682398 None:intergenic
TAATACTTGGAAAGAACTTG+AGG 0.607517 8.1:+10682373 MS.gene036662:CDS
GGTGACATTGTTGGAACAGA+CGG 0.643436 8.1:+10682761 MS.gene036662:CDS
AGGCTTGCACATTGTCACCA+GGG 0.664941 8.1:-10682904 None:intergenic
GAGCATGTATCATTCTGGGA+CGG 0.678978 8.1:+10682827 MS.gene036662:CDS
AAGCAAAGACTCTGTATACA+CGG 0.681751 8.1:-10682876 None:intergenic
TTACTGAAGTATAATGACAG+AGG 0.710690 8.1:+10682794 MS.gene036662:CDS
AGAAGCATAGAGAATATACG+GGG 0.776084 8.1:-10682405 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTGCATTTGATTTAA+TGG + chr8.1:10682502-10682521 MS.gene036662:CDS 15.0%
!! AATTACAATTAGTAGTTGAA+TGG - chr8.1:10682568-10682587 None:intergenic 20.0%
!! TAATGAAAGAGTTGAAATAT+GGG + chr8.1:10682634-10682653 MS.gene036662:CDS 20.0%
!!! AACTACTAATTGTAATTTGT+GGG + chr8.1:10682571-10682590 MS.gene036662:CDS 20.0%
! AATGAAAAAAACCTCAAACA+CGG - chr8.1:10682347-10682366 None:intergenic 25.0%
! CAACTACTAATTGTAATTTG+TGG + chr8.1:10682570-10682589 MS.gene036662:CDS 25.0%
! CTAATGAAAGAGTTGAAATA+TGG + chr8.1:10682633-10682652 MS.gene036662:CDS 25.0%
! TGGAGAATTTCTTAGTTTAA+GGG + chr8.1:10682598-10682617 MS.gene036662:CDS 25.0%
! TGTCATTATACTTCAGTAAT+TGG - chr8.1:10682793-10682812 None:intergenic 25.0%
! TTGGAGAATTTCTTAGTTTA+AGG + chr8.1:10682597-10682616 MS.gene036662:CDS 25.0%
! TTTCATTGAGAGATAATACT+TGG + chr8.1:10682360-10682379 MS.gene036662:CDS 25.0%
!! AATTGTAATTTGTGGGTATT+TGG + chr8.1:10682578-10682597 MS.gene036662:CDS 25.0%
!! TTGCATTTGATTTAATGGAT+CGG + chr8.1:10682507-10682526 MS.gene036662:CDS 25.0%
!!! CATTTTTTGTAGAGCATATT+GGG - chr8.1:10682742-10682761 None:intergenic 25.0%
ACAAAAAATGGTGACATTGT+TGG + chr8.1:10682752-10682771 MS.gene036662:CDS 30.0%
CCAATATGCTCTACAAAAAA+TGG + chr8.1:10682740-10682759 MS.gene036662:CDS 30.0%
TAATACTTGGAAAGAACTTG+AGG + chr8.1:10682373-10682392 MS.gene036662:CDS 30.0%
TTACTGAAGTATAATGACAG+AGG + chr8.1:10682794-10682813 MS.gene036662:CDS 30.0%
! TCAGAAGCATAGAGAATATA+CGG - chr8.1:10682410-10682429 None:intergenic 30.0%
! TCTTTTATGGAGCTATTCAT+TGG + chr8.1:10682447-10682466 MS.gene036662:CDS 30.0%
!!! CCATTTTTTGTAGAGCATAT+TGG - chr8.1:10682743-10682762 None:intergenic 30.0%
AAGCAAAGACTCTGTATACA+CGG - chr8.1:10682879-10682898 None:intergenic 35.0%
AATACAAAGTGCATTCGTCT+TGG + chr8.1:10682666-10682685 MS.gene036662:CDS 35.0%
ACTCTTGTTCTTCCTATTGA+TGG + chr8.1:10682695-10682714 MS.gene036662:CDS 35.0%
TGTAGAGCATATTGGGTAAA+AGG - chr8.1:10682735-10682754 None:intergenic 35.0%
TTATGGAGCTATTCATTGGT+TGG + chr8.1:10682451-10682470 MS.gene036662:CDS 35.0%
TTGTTCGTGGTGATATAAAG+AGG + chr8.1:10682474-10682493 MS.gene036662:CDS 35.0%
! AGAAGCATAGAGAATATACG+GGG - chr8.1:10682408-10682427 None:intergenic 35.0%
! ATGCTTCTGATAACGTAGTA+GGG + chr8.1:10682420-10682439 MS.gene036662:CDS 35.0%
! CAGAAGCATAGAGAATATAC+GGG - chr8.1:10682409-10682428 None:intergenic 35.0%
! TATGCTTCTGATAACGTAGT+AGG + chr8.1:10682419-10682438 MS.gene036662:CDS 35.0%
! TCTAGAGCATGTATCATTCT+GGG + chr8.1:10682823-10682842 MS.gene036662:CDS 35.0%
!! TTCTAGAGCATGTATCATTC+TGG + chr8.1:10682822-10682841 MS.gene036662:CDS 35.0%
AGAGCATATTGGGTAAAAGG+AGG - chr8.1:10682732-10682751 None:intergenic 40.0%
GAGCATATTGGGTAAAAGGA+GGG - chr8.1:10682731-10682750 None:intergenic 40.0%
GTTGAATGGTGAAAACCATC+AGG - chr8.1:10682554-10682573 None:intergenic 40.0%
TAGTTTAAGGGCCAACGATT+GGG + chr8.1:10682610-10682629 MS.gene036662:CDS 40.0%
TCTTTCATTAGCCCAATCGT+TGG - chr8.1:10682624-10682643 None:intergenic 40.0%
TGGTGAAAACCATCAGGAAA+AGG - chr8.1:10682548-10682567 None:intergenic 40.0%
TTAGTTTAAGGGCCAACGAT+TGG + chr8.1:10682609-10682628 MS.gene036662:CDS 40.0%
! ATTCATTGGTTGGTTGTTCG+TGG + chr8.1:10682461-10682480 MS.gene036662:CDS 40.0%
! GTAGTAGGGTCACTCTTTTA+TGG + chr8.1:10682434-10682453 MS.gene036662:CDS 40.0%
! TTAGAGATGCCTTTTCCTGA+TGG + chr8.1:10682536-10682555 MS.gene036662:CDS 40.0%
ACTCTGTATACACGGTCACT+TGG - chr8.1:10682871-10682890 None:intergenic 45.0%
ACTTGGGATCCAAATGGAAG+TGG - chr8.1:10682854-10682873 None:intergenic 45.0%
CTCTGTATACACGGTCACTT+GGG - chr8.1:10682870-10682889 None:intergenic 45.0%
GGAGTCGAAATGCCATCAAT+AGG - chr8.1:10682710-10682729 None:intergenic 45.0%
GGTGACATTGTTGGAACAGA+CGG + chr8.1:10682761-10682780 MS.gene036662:CDS 45.0%
TAGAGAATATACGGGGAGTG+AGG - chr8.1:10682401-10682420 None:intergenic 45.0%
! GAGCATGTATCATTCTGGGA+CGG + chr8.1:10682827-10682846 MS.gene036662:CDS 45.0%
ACGGTCACTTGGGATCCAAA+TGG - chr8.1:10682860-10682879 None:intergenic 50.0%
AGGCTTGCACATTGTCACCA+GGG - chr8.1:10682907-10682926 None:intergenic 50.0%
GAGTCTTTGCTTTCACTCCC+TGG + chr8.1:10682887-10682906 MS.gene036662:CDS 50.0%
TAGGCTTGCACATTGTCACC+AGG - chr8.1:10682908-10682927 None:intergenic 50.0%
TGGGACGGTCCACTTCCATT+TGG + chr8.1:10682842-10682861 MS.gene036662:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 10682335 10682928 10682335 ID=MS.gene036662
chr8.1 mRNA 10682335 10682928 10682335 ID=MS.gene036662.t1;Parent=MS.gene036662
chr8.1 exon 10682335 10682928 10682335 ID=MS.gene036662.t1.exon1;Parent=MS.gene036662.t1
chr8.1 CDS 10682335 10682928 10682335 ID=cds.MS.gene036662.t1;Parent=MS.gene036662.t1
Gene Sequence

>MS.gene036662

ATGGGCTATCCGTGTTTGAGGTTTTTTTCATTGAGAGATAATACTTGGAAAGAACTTGAGGCTCCTCACTCCCCGTATATTCTCTATGCTTCTGATAACGTAGTAGGGTCACTCTTTTATGGAGCTATTCATTGGTTGGTTGTTCGTGGTGATATAAAGAGGCAAGTTATAATTGCATTTGATTTAATGGATCGGAAGCTTTTAGAGATGCCTTTTCCTGATGGTTTTCACCATTCAACTACTAATTGTAATTTGTGGGTATTTGGAGAATTTCTTAGTTTAAGGGCCAACGATTGGGCTAATGAAAGAGTTGAAATATGGGTGATGAACGAATACAAAGTGCATTCGTCTTGGACTAAAACTCTTGTTCTTCCTATTGATGGCATTTCGACTCCCTCCTTTTACCCAATATGCTCTACAAAAAATGGTGACATTGTTGGAACAGACGGTGATATCCAATTACTGAAGTATAATGACAGAGGACAGCTTCTAGAGCATGTATCATTCTGGGACGGTCCACTTCCATTTGGATCCCAAGTGACCGTGTATACAGAGTCTTTGCTTTCACTCCCTGGTGACAATGTGCAAGCCTAA

Protein sequence

>MS.gene036662.t1

MGYPCLRFFSLRDNTWKELEAPHSPYILYASDNVVGSLFYGAIHWLVVRGDIKRQVIIAFDLMDRKLLEMPFPDGFHHSTTNCNLWVFGEFLSLRANDWANERVEIWVMNEYKVHSSWTKTLVLPIDGISTPSFYPICSTKNGDIVGTDGDIQLLKYNDRGQLLEHVSFWDGPLPFGSQVTVYTESLLSLPGDNVQA