AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060367


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060367.t1 MTR_4g118380 95.745 141 6 0 1 141 216 356 1.52e-97 286
MS.gene060367.t1 MTR_4g118400 76.687 163 17 1 1 163 153 294 8.52e-83 246
MS.gene060367.t1 MTR_6g060650 75.460 163 31 1 1 163 214 367 3.17e-82 247
MS.gene060367.t1 MTR_4g117720 69.643 168 36 3 2 163 213 371 4.66e-72 221
MS.gene060367.t1 MTR_3g103020 63.415 164 49 2 2 163 218 372 1.64e-64 202
MS.gene060367.t1 MTR_4g109280 64.198 162 46 3 3 162 201 352 5.59e-64 200
MS.gene060367.t1 MTR_3g034180 61.728 162 52 2 2 163 332 483 2.03e-61 197
MS.gene060367.t1 MTR_3g034130 61.039 154 51 1 2 155 218 362 1.46e-59 189
MS.gene060367.t1 MTR_4g117850 61.078 167 50 4 2 162 216 373 4.79e-59 188
MS.gene060367.t1 MTR_4g109270 60.123 163 54 2 3 163 205 358 2.86e-58 186
MS.gene060367.t1 MTR_4g116320 60.241 166 51 5 2 163 98 252 8.32e-57 179
MS.gene060367.t1 MTR_4g122850 60.000 165 53 5 2 163 204 358 1.71e-55 179
MS.gene060367.t1 MTR_3g034160 61.111 162 53 2 2 163 218 369 2.74e-55 178
MS.gene060367.t1 MTR_1g055195 56.442 163 62 3 1 163 319 472 1.64e-54 179
MS.gene060367.t1 MTR_8g040935 57.143 168 57 4 2 163 44 202 2.57e-54 171
MS.gene060367.t1 MTR_1g055175 56.627 166 58 5 1 163 41 195 8.10e-54 169
MS.gene060367.t1 MTR_1g055165 56.627 166 58 5 1 163 158 312 2.52e-52 169
MS.gene060367.t1 MTR_4g116330 53.892 167 64 3 1 163 217 374 4.28e-52 170
MS.gene060367.t1 MTR_4g094365 54.545 165 63 4 2 163 203 358 6.24e-52 169
MS.gene060367.t1 MTR_5g097210 56.707 164 59 3 2 164 206 358 6.53e-50 164
MS.gene060367.t1 MTR_1g055135 56.364 165 63 4 1 163 212 369 1.15e-49 164
MS.gene060367.t1 MTR_4g023130 54.938 162 59 3 3 162 157 306 2.73e-48 159
MS.gene060367.t1 MTR_4g023090 51.136 176 61 4 3 163 213 378 4.56e-48 160
MS.gene060367.t1 MTR_4g023080 54.321 162 60 4 3 162 198 347 2.11e-47 157
MS.gene060367.t1 MTR_4g117770 53.049 164 69 2 2 163 204 361 6.25e-47 156
MS.gene060367.t1 MTR_4g117810 53.049 164 69 2 2 163 204 361 6.25e-47 156
MS.gene060367.t1 MTR_8g433030 56.886 167 52 8 3 163 184 336 1.03e-46 155
MS.gene060367.t1 MTR_4g117780 52.439 164 70 2 2 163 204 361 1.76e-46 155
MS.gene060367.t1 MTR_8g032390 56.886 167 52 8 3 163 217 369 2.38e-46 155
MS.gene060367.t1 MTR_4g023380 47.283 184 64 3 3 163 198 371 1.21e-45 153
MS.gene060367.t1 MTR_4g117750 54.268 164 71 2 1 164 195 354 2.32e-45 155
MS.gene060367.t1 MTR_4g117750 55.039 129 55 2 37 163 350 477 3.39e-36 130
MS.gene060367.t1 MTR_5g070080 54.268 164 63 3 2 163 226 379 3.49e-45 152
MS.gene060367.t1 MTR_1g055250 52.121 165 61 7 1 162 111 260 6.00e-45 150
MS.gene060367.t1 MTR_4g118370 49.390 164 55 3 1 164 120 255 1.44e-44 147
MS.gene060367.t1 MTR_4g021785 50.000 162 67 3 3 162 199 348 5.57e-43 146
MS.gene060367.t1 MTR_4g023190 53.614 166 62 7 3 163 198 353 5.69e-43 146
MS.gene060367.t1 MTR_4g118360 65.766 111 34 2 55 163 12 120 2.41e-42 137
MS.gene060367.t1 MTR_5g070130 50.311 161 68 3 6 163 219 370 2.97e-42 145
MS.gene060367.t1 MTR_4g023150 53.012 166 63 7 3 163 199 354 3.52e-42 144
MS.gene060367.t1 MTR_4g117870 61.789 123 45 2 42 163 140 261 2.02e-41 139
MS.gene060367.t1 MTR_5g070088 49.398 166 72 3 1 163 223 379 3.67e-41 142
MS.gene060367.t1 MTR_4g023070 47.701 174 67 4 3 162 200 363 3.72e-41 141
MS.gene060367.t1 MTR_4g023200 51.205 166 66 7 3 163 199 354 2.92e-40 139
MS.gene060367.t1 MTR_4g023120 47.429 175 66 5 3 162 200 363 4.43e-40 139
MS.gene060367.t1 MTR_6g463430 50.610 164 64 5 3 163 47 196 2.49e-39 132
MS.gene060367.t1 MTR_8g102520 45.455 165 76 5 2 163 163 316 1.01e-38 134
MS.gene060367.t1 MTR_8g433040 57.639 144 45 5 23 163 228 358 2.20e-37 131
MS.gene060367.t1 MTR_8g032380 57.639 144 45 5 23 163 228 358 2.20e-37 131
MS.gene060367.t1 MTR_7g079400 43.114 167 81 3 2 163 174 331 2.77e-36 128
MS.gene060367.t1 MTR_4g117530 42.945 163 53 2 1 163 125 247 8.29e-34 119
MS.gene060367.t1 MTR_5g023000 64.045 89 32 0 75 163 5 93 3.89e-33 113
MS.gene060367.t1 MTR_7g079370 45.399 163 78 3 2 163 198 350 9.50e-33 119
MS.gene060367.t1 MTR_4g023440 42.236 161 47 2 3 163 212 326 3.36e-31 114
MS.gene060367.t1 MTR_8g098830 44.604 139 67 3 2 140 68 196 3.64e-31 112
MS.gene060367.t1 MTR_7g079450 47.222 144 62 4 21 163 1 131 6.38e-31 108
MS.gene060367.t1 MTR_7g079420 42.262 168 78 7 2 163 984 1138 1.00e-30 117
MS.gene060367.t1 MTR_4g116330 40.719 167 47 3 1 163 217 335 4.10e-28 107
MS.gene060367.t1 MTR_4g122860 50.000 124 49 5 48 163 196 314 5.03e-28 106
MS.gene060367.t1 MTR_2g048580 40.237 169 71 8 2 163 191 336 1.22e-25 100
MS.gene060367.t1 MTR_4g114700 54.444 90 37 2 77 162 132 221 6.53e-23 91.3
MS.gene060367.t1 MTR_4g121580 40.881 159 53 6 3 159 220 339 7.33e-23 93.6
MS.gene060367.t1 MTR_7g117720 52.475 101 43 3 69 164 172 272 1.35e-21 88.6
MS.gene060367.t1 MTR_8g013010 56.522 69 17 1 2 70 254 309 1.89e-15 72.4
MS.gene060367.t1 MTR_4g117430 52.941 68 23 1 2 69 93 151 1.85e-14 67.0
MS.gene060367.t1 MTR_4g122840 35.507 138 44 4 28 163 164 258 1.06e-13 67.0
MS.gene060367.t1 MTR_8g013005 60.377 53 21 0 111 163 1 53 1.53e-13 62.4
MS.gene060367.t1 MTR_4g023430 45.000 80 33 2 91 163 141 216 8.77e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTGTGATTTGTGGGTATT+TGG 0.250931 8.4:+12978686 MS.gene060367:CDS
ATGGTAGGGTCGCTCTTTAA+TGG 0.257647 8.4:+12978515 MS.gene060367:CDS
TGGTAGGGTCGCTCTTTAAT+GGG 0.296490 8.4:+12978516 MS.gene060367:CDS
AAGATGATGTAGTTGAAATA+TGG 0.308827 8.4:+12978735 MS.gene060367:CDS
GCAATTCTCTTTATAGTAGA+AGG 0.334159 8.4:-12978936 None:intergenic
TTGGTGAGTTTCTCAGTGTA+TGG 0.351491 8.4:+12978705 MS.gene060367:CDS
TCTTTAATGGGGCTATTCAC+TGG 0.398766 8.4:+12978528 MS.gene060367:CDS
TAATGGGGCTATTCACTGGT+TGG 0.454880 8.4:+12978532 MS.gene060367:CDS
ATTGGAACTGATGGTGAAGT+TGG 0.456865 8.4:+12978872 MS.gene060367:CDS
TTTCCTTTCAATTAAATCAA+AGG 0.478785 8.4:-12978591 None:intergenic
TTCTCTGATTATTCCATGGT+AGG 0.508178 8.4:+12978500 None:intergenic
TTGCCTTTGATTTAATTGAA+AGG 0.515684 8.4:+12978588 MS.gene060367:CDS
TCTCTGATTATTCCATGGTA+GGG 0.522842 8.4:+12978501 None:intergenic
AACCTACAGATTGTGATTTG+TGG 0.524422 8.4:+12978678 MS.gene060367:CDS
TTGGTGAAGTACGATGAAAA+AGG 0.527194 8.4:+12978896 MS.gene060367:CDS
TAACCTTGTTCGCTATCACC+GGG 0.528671 8.4:-12979002 None:intergenic
ACCTACAGATTGTGATTTGT+GGG 0.529972 8.4:+12978679 MS.gene060367:CDS
AGATGATGTAGTTGAAATAT+GGG 0.540044 8.4:+12978736 MS.gene060367:CDS
AACTGATGGTGAAGTTGGAT+TGG 0.547756 8.4:+12978877 MS.gene060367:CDS
AACCTTGTTCGCTATCACCG+GGG 0.569785 8.4:-12979001 None:intergenic
CTCTATATACATAGCCACTC+GGG 0.570801 8.4:-12978963 None:intergenic
AGTCTTTGCATTCACTCCCC+CGG 0.572586 8.4:+12978984 MS.gene060367:CDS
GGTGATATTATTGGAACTGA+TGG 0.578976 8.4:+12978863 MS.gene060367:CDS
ATTAAAGAGCGACCCTACCA+TGG 0.581861 8.4:-12978513 None:intergenic
AGTACAAAGTGCATTCGTCA+TGG 0.583815 8.4:+12978768 MS.gene060367:CDS
ACTCTATATACATAGCCACT+CGG 0.604656 8.4:-12978964 None:intergenic
ATTGGAGAGAAGTAATGAGT+GGG 0.619618 8.4:-12978824 None:intergenic
ACCTTGTTCGCTATCACCGG+GGG 0.621298 8.4:-12979000 None:intergenic
TATTGGAGAGAAGTAATGAG+TGG 0.628700 8.4:-12978825 None:intergenic
GGTAGGGTCGCTCTTTAATG+GGG 0.635448 8.4:+12978517 MS.gene060367:CDS
GGTTCATGTTCAAAATCACC+TGG 0.645701 8.4:-12978659 None:intergenic
ACCCACAAATCACAATCTGT+AGG 0.659019 8.4:-12978680 None:intergenic
AGAGAATTGCACATCCCGAG+TGG 0.707706 8.4:+12978949 MS.gene060367:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCCTTTCAATTAAATCAA+AGG - chr8.4:12978594-12978613 None:intergenic 20.0%
! AAGATGATGTAGTTGAAATA+TGG + chr8.4:12978735-12978754 MS.gene060367:CDS 25.0%
! ACAAAAAGTGGTGATATTAT+TGG + chr8.4:12978854-12978873 MS.gene060367:CDS 25.0%
! AGATGATGTAGTTGAAATAT+GGG + chr8.4:12978736-12978755 MS.gene060367:CDS 25.0%
!! TTGCCTTTGATTTAATTGAA+AGG + chr8.4:12978588-12978607 MS.gene060367:CDS 25.0%
CCAATATGTTGTACAAAAAG+TGG + chr8.4:12978842-12978861 MS.gene060367:CDS 30.0%
GCAATTCTCTTTATAGTAGA+AGG - chr8.4:12978939-12978958 None:intergenic 30.0%
!!! CCACTTTTTGTACAACATAT+TGG - chr8.4:12978845-12978864 None:intergenic 30.0%
AACCTACAGATTGTGATTTG+TGG + chr8.4:12978678-12978697 MS.gene060367:CDS 35.0%
ACTCTATATACATAGCCACT+CGG - chr8.4:12978967-12978986 None:intergenic 35.0%
ATTGGAGAGAAGTAATGAGT+GGG - chr8.4:12978827-12978846 None:intergenic 35.0%
GGTGATATTATTGGAACTGA+TGG + chr8.4:12978863-12978882 MS.gene060367:CDS 35.0%
TATTGGAGAGAAGTAATGAG+TGG - chr8.4:12978828-12978847 None:intergenic 35.0%
TTGGTGAAGTACGATGAAAA+AGG + chr8.4:12978896-12978915 MS.gene060367:CDS 35.0%
! ACCTACAGATTGTGATTTGT+GGG + chr8.4:12978679-12978698 MS.gene060367:CDS 35.0%
! GATTGTGATTTGTGGGTATT+TGG + chr8.4:12978686-12978705 MS.gene060367:CDS 35.0%
!!! CTTTGCCAGATGATCTTTTT+TGG + chr8.4:12978627-12978646 MS.gene060367:CDS 35.0%
ACCCACAAATCACAATCTGT+AGG - chr8.4:12978683-12978702 None:intergenic 40.0%
AGTACAAAGTGCATTCGTCA+TGG + chr8.4:12978768-12978787 MS.gene060367:CDS 40.0%
CCTCTCCAAAAAAGATCATC+TGG - chr8.4:12978635-12978654 None:intergenic 40.0%
CTCTATATACATAGCCACTC+GGG - chr8.4:12978966-12978985 None:intergenic 40.0%
GGTTCATGTTCAAAATCACC+TGG - chr8.4:12978662-12978681 None:intergenic 40.0%
TCTTTAATGGGGCTATTCAC+TGG + chr8.4:12978528-12978547 MS.gene060367:CDS 40.0%
TTGGTGAGTTTCTCAGTGTA+TGG + chr8.4:12978705-12978724 MS.gene060367:CDS 40.0%
! AACTGATGGTGAAGTTGGAT+TGG + chr8.4:12978877-12978896 MS.gene060367:CDS 40.0%
! ATTGGAACTGATGGTGAAGT+TGG + chr8.4:12978872-12978891 MS.gene060367:CDS 40.0%
!!! CCAGATGATCTTTTTTGGAG+AGG + chr8.4:12978632-12978651 MS.gene060367:CDS 40.0%
ATGGTAGGGTCGCTCTTTAA+TGG + chr8.4:12978515-12978534 MS.gene060367:CDS 45.0%
TAATGGGGCTATTCACTGGT+TGG + chr8.4:12978532-12978551 MS.gene060367:CDS 45.0%
TGGTAGGGTCGCTCTTTAAT+GGG + chr8.4:12978516-12978535 MS.gene060367:CDS 45.0%
!!! CTTTTTTGGAGAGGTTTGCC+AGG + chr8.4:12978641-12978660 MS.gene060367:CDS 45.0%
AGAGAATTGCACATCCCGAG+TGG + chr8.4:12978949-12978968 MS.gene060367:CDS 50.0%
AGTCTTTGCATTCACTCCCC+CGG + chr8.4:12978984-12979003 MS.gene060367:CDS 50.0%
GGTAGGGTCGCTCTTTAATG+GGG + chr8.4:12978517-12978536 MS.gene060367:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 12978515 12979009 12978515 ID=MS.gene060367
chr8.4 mRNA 12978515 12979009 12978515 ID=MS.gene060367.t1;Parent=MS.gene060367
chr8.4 exon 12978515 12979009 12978515 ID=MS.gene060367.t1.exon1;Parent=MS.gene060367.t1
chr8.4 CDS 12978515 12979009 12978515 ID=cds.MS.gene060367.t1;Parent=MS.gene060367.t1
Gene Sequence

>MS.gene060367

ATGGTAGGGTCGCTCTTTAATGGGGCTATTCACTGGTTGGCTTTTCATCTTGATTTATCAATGAATGTTATTGTTGCCTTTGATTTAATTGAAAGGAAACTTTTAGACATATCTTTGCCAGATGATCTTTTTTGGAGAGGTTTGCCAGGTGATTTTGAACATGAACCTACAGATTGTGATTTGTGGGTATTTGGTGAGTTTCTCAGTGTATGGACTAAGCAAGATGATGTAGTTGAAATATGGGTGATGAAAGAGTACAAAGTGCATTCGTCATGGACTAAGACTCTTGTTTTTTCTATTGATGTCATTCCCACTCATTACTTCTCTCCAATATGTTGTACAAAAAGTGGTGATATTATTGGAACTGATGGTGAAGTTGGATTGGTGAAGTACGATGAAAAAGGACAGTTTCTAGAGTATACCTTCTACTATAAAGAGAATTGCACATCCCGAGTGGCTATGTATATAGAGTCTTTGCATTCACTCCCCCGGTGA

Protein sequence

>MS.gene060367.t1

MVGSLFNGAIHWLAFHLDLSMNVIVAFDLIERKLLDISLPDDLFWRGLPGDFEHEPTDCDLWVFGEFLSVWTKQDDVVEIWVMKEYKVHSSWTKTLVFSIDVIPTHYFSPICCTKSGDIIGTDGEVGLVKYDEKGQFLEYTFYYKENCTSRVAMYIESLHSLPR