AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48221

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48221.t1 MTR_4g023070 81.781 247 44 1 1 246 127 373 8.97e-144 408
MS.gene48221.t1 MTR_4g023120 81.148 244 45 1 1 243 127 370 4.91e-141 401
MS.gene48221.t1 MTR_4g023090 58.403 238 90 4 10 243 154 386 2.67e-88 268
MS.gene48221.t1 MTR_4g021785 56.098 246 82 4 1 243 133 355 7.29e-86 260
MS.gene48221.t1 MTR_4g023130 57.377 244 79 5 1 242 92 312 8.64e-85 256
MS.gene48221.t1 MTR_4g023380 55.039 258 60 5 10 243 154 379 4.87e-84 256
MS.gene48221.t1 MTR_4g023080 56.967 244 80 5 1 242 133 353 4.88e-84 256
MS.gene48221.t1 MTR_4g023190 53.279 244 91 5 1 241 134 357 4.14e-74 230
MS.gene48221.t1 MTR_4g023200 52.245 245 93 6 1 241 134 358 1.01e-70 222
MS.gene48221.t1 MTR_3g103020 53.750 240 84 7 13 243 157 378 2.64e-70 221
MS.gene48221.t1 MTR_4g023150 51.837 245 94 6 1 241 134 358 1.59e-69 219
MS.gene48221.t1 MTR_6g060650 51.489 235 92 7 12 242 156 372 7.23e-68 215
MS.gene48221.t1 MTR_4g116330 49.593 246 100 8 1 240 150 377 8.68e-67 212
MS.gene48221.t1 MTR_8g032390 52.282 241 90 9 3 238 150 370 2.55e-65 208
MS.gene48221.t1 MTR_8g433030 53.478 230 85 8 14 238 125 337 2.58e-65 207
MS.gene48221.t1 MTR_4g117720 52.083 240 86 8 12 240 154 375 2.75e-65 208
MS.gene48221.t1 MTR_4g094365 50.000 238 95 7 11 243 146 364 9.31e-64 204
MS.gene48221.t1 MTR_4g023440 47.951 244 71 5 1 243 144 332 2.67e-61 197
MS.gene48221.t1 MTR_4g109280 50.962 208 79 6 43 243 168 359 1.20e-60 196
MS.gene48221.t1 MTR_4g122850 47.566 267 107 12 1 253 127 374 3.84e-60 195
MS.gene48221.t1 MTR_6g463430 48.927 233 82 5 16 243 2 202 2.06e-59 187
MS.gene48221.t1 MTR_8g433040 48.739 238 90 6 11 243 154 364 7.77e-59 191
MS.gene48221.t1 MTR_8g032380 48.739 238 90 6 11 243 154 364 7.77e-59 191
MS.gene48221.t1 MTR_4g109270 47.899 238 96 6 12 243 149 364 4.15e-58 189
MS.gene48221.t1 MTR_4g117850 50.622 241 89 10 12 240 154 376 5.69e-58 189
MS.gene48221.t1 MTR_3g034180 45.669 254 110 9 2 243 252 489 7.81e-56 187
MS.gene48221.t1 MTR_5g097210 47.200 250 108 8 11 253 141 373 9.65e-56 184
MS.gene48221.t1 MTR_4g116320 48.472 229 96 5 18 240 43 255 2.18e-55 179
MS.gene48221.t1 MTR_4g118380 49.315 219 84 5 10 218 156 357 1.15e-54 180
MS.gene48221.t1 MTR_4g117770 46.250 240 103 6 11 243 147 367 7.74e-54 178
MS.gene48221.t1 MTR_4g117810 46.250 240 103 6 11 243 147 367 7.74e-54 178
MS.gene48221.t1 MTR_3g034130 45.923 233 99 7 2 223 138 354 1.69e-53 177
MS.gene48221.t1 MTR_1g055195 44.094 254 102 7 9 249 258 484 6.59e-53 179
MS.gene48221.t1 MTR_4g117870 43.496 246 86 9 1 243 72 267 7.24e-53 173
MS.gene48221.t1 MTR_4g117780 45.000 240 106 6 11 243 147 367 3.02e-52 174
MS.gene48221.t1 MTR_3g034160 45.817 251 108 8 2 240 138 372 3.05e-52 174
MS.gene48221.t1 MTR_8g040935 49.524 210 84 7 43 243 12 208 6.42e-51 166
MS.gene48221.t1 MTR_5g070088 44.915 236 108 6 13 241 163 383 7.09e-51 171
MS.gene48221.t1 MTR_5g070130 43.359 256 115 9 12 253 148 387 1.31e-49 168
MS.gene48221.t1 MTR_1g055135 44.534 247 107 9 10 243 146 375 1.78e-47 162
MS.gene48221.t1 MTR_5g070080 47.186 231 100 8 13 238 167 380 7.00e-47 161
MS.gene48221.t1 MTR_4g118400 47.236 199 74 5 42 236 122 293 3.14e-46 157
MS.gene48221.t1 MTR_4g116330 41.463 246 81 8 1 240 150 338 7.71e-46 157
MS.gene48221.t1 MTR_4g118370 42.917 240 94 10 9 240 53 257 2.08e-45 153
MS.gene48221.t1 MTR_1g055250 37.407 270 138 8 10 268 46 295 6.26e-44 152
MS.gene48221.t1 MTR_7g079370 41.702 235 111 7 12 236 131 349 2.37e-43 151
MS.gene48221.t1 MTR_4g121580 43.515 239 83 10 11 242 155 348 3.65e-43 151
MS.gene48221.t1 MTR_1g055175 42.326 215 100 6 37 242 1 200 4.42e-43 145
MS.gene48221.t1 MTR_4g117750 42.292 253 115 8 9 247 128 363 2.59e-39 143
MS.gene48221.t1 MTR_4g117750 45.833 144 57 3 107 241 350 481 5.66e-26 106
MS.gene48221.t1 MTR_7g079400 38.866 247 117 9 12 243 110 337 2.90e-38 137
MS.gene48221.t1 MTR_1g055165 46.512 172 74 4 75 243 162 318 9.49e-38 135
MS.gene48221.t1 MTR_4g117530 39.149 235 88 5 11 243 72 253 1.13e-37 133
MS.gene48221.t1 MTR_8g102520 36.220 254 132 9 1 243 88 322 1.22e-35 130
MS.gene48221.t1 MTR_4g114700 35.632 261 90 7 12 263 50 241 1.07e-29 112
MS.gene48221.t1 MTR_4g122860 38.140 215 93 8 51 260 158 337 3.39e-29 113
MS.gene48221.t1 MTR_4g117430 49.194 124 48 4 12 132 41 152 8.61e-28 105
MS.gene48221.t1 MTR_5g023000 53.636 110 37 2 136 243 2 99 3.94e-27 101
MS.gene48221.t1 MTR_8g013010 49.206 126 48 5 12 132 195 309 9.40e-27 106
MS.gene48221.t1 MTR_4g118360 46.043 139 54 4 107 240 1 123 2.25e-26 100
MS.gene48221.t1 MTR_4g023430 57.447 94 23 2 155 243 141 222 1.15e-25 101
MS.gene48221.t1 MTR_7g079420 34.836 244 94 12 12 241 951 1143 1.41e-25 106
MS.gene48221.t1 MTR_2g048580 34.862 218 107 9 30 236 142 335 2.50e-25 102
MS.gene48221.t1 MTR_8g098830 38.202 178 84 8 45 214 35 194 1.32e-24 98.6
MS.gene48221.t1 MTR_7g079450 39.103 156 73 5 91 243 1 137 2.39e-24 95.5
MS.gene48221.t1 MTR_7g117720 51.852 108 34 3 135 236 175 270 1.17e-21 91.7
MS.gene48221.t1 MTR_7g117720 44.706 85 36 3 11 88 132 212 7.42e-14 70.1
MS.gene48221.t1 MTR_6g034720 46.465 99 50 2 12 108 72 169 3.52e-19 84.0
MS.gene48221.t1 MTR_4g023100 72.222 54 15 0 190 243 66 119 5.52e-19 80.9
MS.gene48221.t1 MTR_1g094210 28.099 242 143 9 10 245 192 408 6.76e-19 85.9
MS.gene48221.t1 MTR_4g116340 48.864 88 40 3 12 97 163 247 7.61e-18 80.9
MS.gene48221.t1 MTR_1g094210 29.851 201 117 7 10 208 192 370 3.38e-17 80.9
MS.gene48221.t1 MTR_2g021050 28.821 229 126 11 1 212 119 327 4.74e-17 80.5
MS.gene48221.t1 MTR_4g133170 29.048 210 111 10 12 208 142 326 5.16e-16 77.4
MS.gene48221.t1 MTR_8g013005 60.000 55 20 1 190 242 4 58 6.29e-14 65.5
MS.gene48221.t1 MTR_2g425980 25.275 273 171 10 10 267 156 410 5.19e-13 68.6
MS.gene48221.t1 MTR_2g426020 27.413 259 153 10 10 253 158 396 5.38e-13 68.6
MS.gene48221.t1 MTR_1g068860 28.652 178 109 8 12 178 141 311 2.88e-12 66.2
MS.gene48221.t1 MTR_2g078870 27.984 243 143 11 1 235 89 307 3.38e-12 65.9
MS.gene48221.t1 MTR_2g021155 29.557 203 122 9 11 208 171 357 4.17e-12 66.6
MS.gene48221.t1 MTR_3g007360 30.802 237 130 11 10 236 191 403 4.18e-12 65.9
MS.gene48221.t1 MTR_2g028140 30.556 180 105 8 12 178 167 339 1.07e-11 64.7
MS.gene48221.t1 MTR_2g078900 30.909 165 98 7 1 163 164 314 1.22e-11 64.3
MS.gene48221.t1 MTR_0667s0010 26.512 215 126 9 12 213 152 347 1.72e-11 64.3
MS.gene48221.t1 MTR_3g007410 30.380 237 131 11 10 236 191 403 2.92e-11 63.5
MS.gene48221.t1 MTR_4g109290 62.264 53 18 1 193 243 136 188 3.64e-11 61.2
MS.gene48221.t1 MTR_2g024100 30.000 180 96 9 9 170 151 318 5.09e-11 62.8
MS.gene48221.t1 MTR_8g099460 30.519 154 99 6 12 162 153 301 5.16e-11 62.8
MS.gene48221.t1 MTR_1g097560 23.451 226 143 8 13 235 175 373 5.42e-11 62.8
MS.gene48221.t1 MTR_3g030320 29.677 155 89 6 13 163 90 228 6.06e-11 62.0
MS.gene48221.t1 MTR_3g023930 27.660 235 124 9 11 235 169 367 6.44e-11 62.4
MS.gene48221.t1 MTR_7g034310 26.122 245 137 9 8 236 12 228 8.01e-11 60.8
MS.gene48221.t1 MTR_2g055970 26.609 233 146 8 10 235 346 560 9.17e-11 62.4
MS.gene48221.t1 MTR_3g080370 29.389 262 150 10 13 243 168 425 9.21e-11 62.0
MS.gene48221.t1 MTR_3g083640 27.273 231 134 11 12 235 199 402 9.82e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48221.t1 AT3G06240 28.017 232 132 9 13 235 214 419 4.03e-13 69.3

Find 55 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCTTCTCCTTTGTTATTA+TGG 0.085899 4.2:-77876544 None:intergenic
CATTGTTGGCTTTGATTTAA+TGG 0.146472 4.2:+77876625 MS.gene48221:CDS
TAAGAATGGATTACATTTAT+TGG 0.163732 4.2:-77876435 None:intergenic
CATTCTGGTTTGTGGGTATT+TGG 0.166612 4.2:+77876704 MS.gene48221:CDS
ACAGAAAATGGTGATATTAT+TGG 0.182801 4.2:+77876914 MS.gene48221:CDS
TTAATGATACAACTGAAATA+TGG 0.214098 4.2:+77876759 MS.gene48221:CDS
AAATGTAATCCATTCTTAAT+TGG 0.219945 4.2:+77876440 MS.gene48221:CDS
TTGGAGAATTTCTCAGTATA+TGG 0.239627 4.2:+77876723 MS.gene48221:CDS
CCTTTGTTATTATGGATATA+AGG 0.243299 4.2:-77876536 None:intergenic
CCTATATATTACACAGAAAA+TGG 0.262547 4.2:+77876902 MS.gene48221:CDS
TATATTAATCTATATGGTTT+TGG 0.287874 4.2:+77876371 MS.gene48221:CDS
AAACCGTATATTAATCTATA+TGG 0.293548 4.2:+77876365 MS.gene48221:CDS
GTGAAAGTTGTCCACAGTTC+TGG 0.304201 4.2:-77876879 None:intergenic
CACAAAGCATTAGTACATTC+TGG 0.312868 4.2:+77876689 MS.gene48221:CDS
GCTTTGTGATCAACATCATT+TGG 0.364371 4.2:-77876674 None:intergenic
TAATGATACAACTGAAATAT+GGG 0.366787 4.2:+77876760 MS.gene48221:CDS
TGGTGTTCCTCAATCACATT+TGG 0.386774 4.2:+77876460 MS.gene48221:CDS
AAACCATATAGATTAATATA+CGG 0.396325 4.2:-77876368 None:intergenic
TTCGCGTTGCTATATAAGTT+TGG 0.401593 4.2:+77876598 MS.gene48221:CDS
TGGTGACAATGAGCATGTTT+TGG 0.401853 4.2:+77877057 MS.gene48221:CDS
TCTCGTTTAGAGACGATTCA+TGG 0.402594 4.2:+77876489 MS.gene48221:CDS
ATAAGTTTGGATGTCATTGT+TGG 0.406232 4.2:+77876611 MS.gene48221:CDS
TGGAGAATTTCTCAGTATAT+GGG 0.411388 4.2:+77876724 MS.gene48221:CDS
ATTTCTCAGTATATGGGTTA+AGG 0.418550 4.2:+77876730 MS.gene48221:CDS
TAATGGGGTTATTCATTGGT+TGG 0.419885 4.2:+77876574 MS.gene48221:CDS
CATTAGTACATTCTGGTTTG+TGG 0.428815 4.2:+77876696 MS.gene48221:CDS
GGTGATATTATTGGAAAATA+CGG 0.431474 4.2:+77876923 MS.gene48221:CDS
ATTAGTACATTCTGGTTTGT+GGG 0.435518 4.2:+77876697 MS.gene48221:CDS
ACCACATAATCATCTCTAGA+TGG 0.448654 4.2:-77876401 None:intergenic
GAGTCTCTACTTTCACTCTC+TGG 0.466024 4.2:+77877037 MS.gene48221:CDS
TGAGGAACACCAATTAAGAA+TGG 0.466077 4.2:-77876449 None:intergenic
AGAAGGTCAAAGTCATGATC+AGG 0.469359 4.2:-77876848 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.494278 4.2:+77876792 MS.gene48221:CDS
CAGTTCTGGAATAATATAGA+AGG 0.497627 4.2:-77876865 None:intergenic
ATCCATAATAACAAAGGAGA+AGG 0.499756 4.2:+77876542 MS.gene48221:CDS
TCCATAATAACAAAGGAGAA+GGG 0.503906 4.2:+77876543 MS.gene48221:CDS
AAAATACGGTGGTACTAGAT+TGG 0.512046 4.2:+77876937 MS.gene48221:CDS
CTCCTTCTGCAACAGTCCAT+CGG 0.533619 4.2:+77876997 MS.gene48221:CDS
TTGGCTTTGATTTAATGGAA+AGG 0.539588 4.2:+77876630 MS.gene48221:CDS
TGATCAACATCATTTGGAAG+AGG 0.539905 4.2:-77876668 None:intergenic
GTATACACGACGACTTCCGA+TGG 0.565734 4.2:-77877013 None:intergenic
TTAGTATTAAAAGCAGACAT+TGG 0.581460 4.2:-77876344 None:intergenic
TTCCGATGGACTGTTGCAGA+AGG 0.589220 4.2:-77876999 None:intergenic
TTGGTGAAGTATAATGACAA+AGG 0.592959 4.2:+77876956 MS.gene48221:CDS
CCATAATAACAAAGGAGAAG+GGG 0.599927 4.2:+77876544 MS.gene48221:CDS
CGATTCATGGAAAGAAATCG+AGG 0.603536 4.2:+77876502 MS.gene48221:CDS
TCTATATTATTCCAGAACTG+TGG 0.619949 4.2:+77876868 MS.gene48221:CDS
TGATCAGGAATGACATGCTG+AGG 0.624130 4.2:-77876833 None:intergenic
TAATAACAAAGGAGAAGGGG+TGG 0.624815 4.2:+77876547 MS.gene48221:CDS
CCTTATATCCATAATAACAA+AGG 0.629106 4.2:+77876536 MS.gene48221:CDS
TCCATCTAGAGATGATTATG+TGG 0.669252 4.2:+77876400 MS.gene48221:CDS
GATTCATGGAAAGAAATCGA+GGG 0.678761 4.2:+77876503 MS.gene48221:CDS
GATATTATTGGAAAATACGG+TGG 0.691433 4.2:+77876926 MS.gene48221:CDS
TGGCAGAAACAAGAACAATG+AGG 0.702406 4.2:+77877084 MS.gene48221:CDS
ATCTAGAGATGATTATGTGG+TGG 0.726328 4.2:+77876403 MS.gene48221:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACCATATAGATTAATATA+CGG - chr4.2:77876371-77876390 None:intergenic 15.0%
!!! TATATTAATCTATATGGTTT+TGG + chr4.2:77876371-77876390 MS.gene48221:CDS 15.0%
!! AAACCGTATATTAATCTATA+TGG + chr4.2:77876365-77876384 MS.gene48221:CDS 20.0%
!! AAATGTAATCCATTCTTAAT+TGG + chr4.2:77876440-77876459 MS.gene48221:CDS 20.0%
!! TAATGATACAACTGAAATAT+GGG + chr4.2:77876760-77876779 MS.gene48221:CDS 20.0%
!! TTAATGATACAACTGAAATA+TGG + chr4.2:77876759-77876778 MS.gene48221:CDS 20.0%
!!! CATTTTCTGTGTAATATATA+GGG - chr4.2:77876904-77876923 None:intergenic 20.0%
!!! TAAGAATGGATTACATTTAT+TGG - chr4.2:77876438-77876457 None:intergenic 20.0%
! ACAGAAAATGGTGATATTAT+TGG + chr4.2:77876914-77876933 MS.gene48221:CDS 25.0%
! CCTATATATTACACAGAAAA+TGG + chr4.2:77876902-77876921 MS.gene48221:CDS 25.0%
! CCTTATATCCATAATAACAA+AGG + chr4.2:77876536-77876555 MS.gene48221:CDS 25.0%
! GGTGATATTATTGGAAAATA+CGG + chr4.2:77876923-77876942 MS.gene48221:CDS 25.0%
!! CCATTTTCTGTGTAATATAT+AGG - chr4.2:77876905-77876924 None:intergenic 25.0%
!! TTTTTAATGGGGTTATTCAT+TGG + chr4.2:77876570-77876589 MS.gene48221:CDS 25.0%
!!! CCTTTGTTATTATGGATATA+AGG - chr4.2:77876539-77876558 None:intergenic 25.0%
AAAAACTCCAAATGTGATTG+AGG - chr4.2:77876470-77876489 None:intergenic 30.0%
ATAAGTTTGGATGTCATTGT+TGG + chr4.2:77876611-77876630 MS.gene48221:CDS 30.0%
ATCCATAATAACAAAGGAGA+AGG + chr4.2:77876542-77876561 MS.gene48221:CDS 30.0%
GATATTATTGGAAAATACGG+TGG + chr4.2:77876926-77876945 MS.gene48221:CDS 30.0%
TCCATAATAACAAAGGAGAA+GGG + chr4.2:77876543-77876562 MS.gene48221:CDS 30.0%
TCTATATTATTCCAGAACTG+TGG + chr4.2:77876868-77876887 MS.gene48221:CDS 30.0%
TGGAGAATTTCTCAGTATAT+GGG + chr4.2:77876724-77876743 MS.gene48221:CDS 30.0%
TTGGAGAATTTCTCAGTATA+TGG + chr4.2:77876723-77876742 MS.gene48221:CDS 30.0%
TTGGTGAAGTATAATGACAA+AGG + chr4.2:77876956-77876975 MS.gene48221:CDS 30.0%
! AATGACAAAGGAGAGTTTTT+AGG + chr4.2:77876968-77876987 MS.gene48221:CDS 30.0%
! ATGACAAAGGAGAGTTTTTA+GGG + chr4.2:77876969-77876988 MS.gene48221:CDS 30.0%
! ATTTCTCAGTATATGGGTTA+AGG + chr4.2:77876730-77876749 MS.gene48221:CDS 30.0%
!! ATTAGTACATTCTGGTTTGT+GGG + chr4.2:77876697-77876716 MS.gene48221:CDS 30.0%
!! CAGTTCTGGAATAATATAGA+AGG - chr4.2:77876868-77876887 None:intergenic 30.0%
!! CATTGTTGGCTTTGATTTAA+TGG + chr4.2:77876625-77876644 MS.gene48221:CDS 30.0%
!! TTGGCTTTGATTTAATGGAA+AGG + chr4.2:77876630-77876649 MS.gene48221:CDS 30.0%
AAAATACGGTGGTACTAGAT+TGG + chr4.2:77876937-77876956 MS.gene48221:CDS 35.0%
AATACAAAGTGCATTCGTCT+TGG + chr4.2:77876792-77876811 MS.gene48221:CDS 35.0%
ACCACATAATCATCTCTAGA+TGG - chr4.2:77876404-77876423 None:intergenic 35.0%
ATCTAGAGATGATTATGTGG+TGG + chr4.2:77876403-77876422 MS.gene48221:CDS 35.0%
CCATAATAACAAAGGAGAAG+GGG + chr4.2:77876544-77876563 MS.gene48221:CDS 35.0%
GATTCATGGAAAGAAATCGA+GGG + chr4.2:77876503-77876522 MS.gene48221:CDS 35.0%
GCTTTGTGATCAACATCATT+TGG - chr4.2:77876677-77876696 None:intergenic 35.0%
TAATGGGGTTATTCATTGGT+TGG + chr4.2:77876574-77876593 MS.gene48221:CDS 35.0%
TCCATCTAGAGATGATTATG+TGG + chr4.2:77876400-77876419 MS.gene48221:CDS 35.0%
TGAGGAACACCAATTAAGAA+TGG - chr4.2:77876452-77876471 None:intergenic 35.0%
TGATCAACATCATTTGGAAG+AGG - chr4.2:77876671-77876690 None:intergenic 35.0%
TTCGCGTTGCTATATAAGTT+TGG + chr4.2:77876598-77876617 MS.gene48221:CDS 35.0%
! CACAAAGCATTAGTACATTC+TGG + chr4.2:77876689-77876708 MS.gene48221:CDS 35.0%
!! CATTAGTACATTCTGGTTTG+TGG + chr4.2:77876696-77876715 MS.gene48221:CDS 35.0%
!!! AATGAGCATGTTTTGGAAGA+TGG + chr4.2:77877064-77877083 MS.gene48221:CDS 35.0%
AGAAGGTCAAAGTCATGATC+AGG - chr4.2:77876851-77876870 None:intergenic 40.0%
CGATTCATGGAAAGAAATCG+AGG + chr4.2:77876502-77876521 MS.gene48221:CDS 40.0%
TAATAACAAAGGAGAAGGGG+TGG + chr4.2:77876547-77876566 MS.gene48221:CDS 40.0%
TCTCGTTTAGAGACGATTCA+TGG + chr4.2:77876489-77876508 MS.gene48221:CDS 40.0%
TGGCAGAAACAAGAACAATG+AGG + chr4.2:77877084-77877103 MS.gene48221:CDS 40.0%
TGGTGTTCCTCAATCACATT+TGG + chr4.2:77876460-77876479 MS.gene48221:CDS 40.0%
! AGAAGGGGTGGTTTTTAATG+GGG + chr4.2:77876559-77876578 MS.gene48221:CDS 40.0%
! GAGAAGGGGTGGTTTTTAAT+GGG + chr4.2:77876558-77876577 MS.gene48221:CDS 40.0%
! TGGTGACAATGAGCATGTTT+TGG + chr4.2:77877057-77877076 MS.gene48221:CDS 40.0%
!! CATTCTGGTTTGTGGGTATT+TGG + chr4.2:77876704-77876723 MS.gene48221:CDS 40.0%
!! CCCCTTCTCCTTTGTTATTA+TGG - chr4.2:77876547-77876566 None:intergenic 40.0%
GAGTCTCTACTTTCACTCTC+TGG + chr4.2:77877037-77877056 MS.gene48221:CDS 45.0%
GTGAAAGTTGTCCACAGTTC+TGG - chr4.2:77876882-77876901 None:intergenic 45.0%
TGATCAGGAATGACATGCTG+AGG - chr4.2:77876836-77876855 None:intergenic 45.0%
! GGAGAAGGGGTGGTTTTTAA+TGG + chr4.2:77876557-77876576 MS.gene48221:CDS 45.0%
CTCCTTCTGCAACAGTCCAT+CGG + chr4.2:77876997-77877016 MS.gene48221:CDS 50.0%
GTATACACGACGACTTCCGA+TGG - chr4.2:77877016-77877035 None:intergenic 50.0%
!! TTCCGATGGACTGTTGCAGA+AGG - chr4.2:77877002-77877021 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 77876347 77877159 77876347 ID=MS.gene48221
chr4.2 mRNA 77876347 77877159 77876347 ID=MS.gene48221.t1;Parent=MS.gene48221
chr4.2 exon 77876347 77877159 77876347 ID=MS.gene48221.t1.exon1;Parent=MS.gene48221.t1
chr4.2 CDS 77876347 77877159 77876347 ID=cds.MS.gene48221.t1;Parent=MS.gene48221.t1
Gene Sequence

>MS.gene48221

ATGTCTGCTTTTAATACTAAACCGTATATTAATCTATATGGTTTTGGTTATGATCCATCTAGAGATGATTATGTGGTGGTTGCATTGTCCAATAAATGTAATCCATTCTTAATTGGTGTTCCTCAATCACATTTGGAGTTTTTCTCGTTTAGAGACGATTCATGGAAAGAAATCGAGGGTGCTCACTTACCTTATATCCATAATAACAAAGGAGAAGGGGTGGTTTTTAATGGGGTTATTCATTGGTTGGCTTCGCGTTGCTATATAAGTTTGGATGTCATTGTTGGCTTTGATTTAATGGAAAGGAAATTGTTTGATCTTCCTCTTCCAAATGATGTTGATCACAAAGCATTAGTACATTCTGGTTTGTGGGTATTTGGAGAATTTCTCAGTATATGGGTTAAGGATATTTTTAATGATACAACTGAAATATGGGTGATGAAAGAATACAAAGTGCATTCGTCTTGGACTAAGACTCTTGTTCTTCCTCAGCATGTCATTCCTGATCATGACTTTGACCTTCTATATTATTCCAGAACTGTGGACAACTTTCACCCTATATATTACACAGAAAATGGTGATATTATTGGAAAATACGGTGGTACTAGATTGGTGAAGTATAATGACAAAGGAGAGTTTTTAGGGCATCACTCCTTCTGCAACAGTCCATCGGAAGTCGTCGTGTATACAGAGTCTCTACTTTCACTCTCTGGTGACAATGAGCATGTTTTGGAAGATGGCAGAAACAAGAACAATGAGGTTGTGAAATATTCTGCTTCATTGTGTTATTTATATTGTATTATGAACTCTTGA
Protein sequence

>MS.gene48221.t1

MSAFNTKPYINLYGFGYDPSRDDYVVVALSNKCNPFLIGVPQSHLEFFSFRDDSWKEIEGAHLPYIHNNKGEGVVFNGVIHWLASRCYISLDVIVGFDLMERKLFDLPLPNDVDHKALVHSGLWVFGEFLSIWVKDIFNDTTEIWVMKEYKVHSSWTKTLVLPQHVIPDHDFDLLYYSRTVDNFHPIYYTENGDIIGKYGGTRLVKYNDKGEFLGHHSFCNSPSEVVVYTESLLSLSGDNEHVLEDGRNKNNEVVKYSASLCYLYCIMNS