AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene019504


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019504.t1 MTR_8g433040 82.308 130 23 0 1 130 235 364 3.54e-70 214
MS.gene019504.t1 MTR_8g032380 82.308 130 23 0 1 130 235 364 3.54e-70 214
MS.gene019504.t1 MTR_8g433030 79.200 125 25 1 1 124 212 336 6.92e-65 200
MS.gene019504.t1 MTR_8g032390 79.200 125 25 1 1 124 245 369 1.53e-64 200
MS.gene019504.t1 MTR_4g094365 64.925 134 43 2 1 130 231 364 5.84e-50 162
MS.gene019504.t1 MTR_3g103020 63.433 134 44 3 1 130 246 378 1.72e-49 162
MS.gene019504.t1 MTR_4g109270 60.448 134 48 3 1 130 232 364 1.83e-46 154
MS.gene019504.t1 MTR_4g116320 61.538 130 45 2 1 126 126 254 2.96e-45 147
MS.gene019504.t1 MTR_6g060650 58.647 133 48 4 1 129 243 372 8.81e-44 147
MS.gene019504.t1 MTR_4g122850 62.687 134 45 4 1 130 232 364 1.57e-43 146
MS.gene019504.t1 MTR_5g097210 60.156 128 47 2 2 127 235 360 2.06e-42 143
MS.gene019504.t1 MTR_4g117720 58.647 133 43 4 1 124 242 371 4.60e-42 142
MS.gene019504.t1 MTR_8g040935 56.716 134 53 2 2 130 75 208 8.75e-42 137
MS.gene019504.t1 MTR_4g109280 58.824 136 46 6 1 130 228 359 1.60e-41 140
MS.gene019504.t1 MTR_4g116330 56.296 135 54 2 1 130 246 380 1.64e-41 141
MS.gene019504.t1 MTR_1g055175 53.788 132 57 2 1 129 70 200 1.83e-41 136
MS.gene019504.t1 MTR_4g023130 58.015 131 51 3 1 129 184 312 1.03e-39 135
MS.gene019504.t1 MTR_3g034180 58.647 133 49 4 1 130 360 489 1.14e-39 138
MS.gene019504.t1 MTR_1g055165 53.383 133 58 2 1 130 187 318 1.88e-39 134
MS.gene019504.t1 MTR_4g117850 56.693 127 49 3 2 123 248 373 7.62e-39 134
MS.gene019504.t1 MTR_4g023080 55.725 131 54 3 1 129 225 353 1.08e-38 133
MS.gene019504.t1 MTR_4g021785 54.545 132 56 3 1 130 226 355 3.11e-38 132
MS.gene019504.t1 MTR_4g023380 51.282 156 49 5 1 130 225 379 1.90e-37 130
MS.gene019504.t1 MTR_4g118370 53.488 129 55 3 1 127 132 257 7.42e-37 126
MS.gene019504.t1 MTR_6g463430 53.383 133 55 4 1 130 74 202 1.16e-36 124
MS.gene019504.t1 MTR_4g023090 52.857 140 49 5 1 124 240 378 1.40e-36 128
MS.gene019504.t1 MTR_4g118360 55.469 128 48 5 8 129 1 125 1.58e-36 121
MS.gene019504.t1 MTR_1g055135 51.825 137 57 4 1 130 241 375 4.72e-36 127
MS.gene019504.t1 MTR_4g023070 51.049 143 54 3 2 129 228 369 1.03e-35 126
MS.gene019504.t1 MTR_4g117870 52.344 128 58 2 5 130 141 267 2.87e-35 122
MS.gene019504.t1 MTR_5g070130 54.264 129 53 4 1 124 243 370 6.94e-35 124
MS.gene019504.t1 MTR_1g055195 54.545 132 45 5 1 124 348 472 7.98e-35 125
MS.gene019504.t1 MTR_5g070080 54.331 127 52 4 2 124 255 379 2.04e-34 123
MS.gene019504.t1 MTR_1g055250 49.624 133 61 3 2 130 140 270 5.88e-33 118
MS.gene019504.t1 MTR_4g023120 48.611 144 58 3 2 130 228 370 1.53e-32 117
MS.gene019504.t1 MTR_3g034160 56.489 131 53 3 1 129 246 374 1.65e-32 117
MS.gene019504.t1 MTR_4g118380 57.895 114 34 4 1 103 245 355 5.35e-32 116
MS.gene019504.t1 MTR_3g034130 54.167 120 50 3 1 117 246 363 6.99e-32 115
MS.gene019504.t1 MTR_4g117750 54.386 114 49 2 18 128 368 481 7.61e-32 117
MS.gene019504.t1 MTR_4g117750 48.624 109 51 3 23 129 253 358 7.96e-24 95.5
MS.gene019504.t1 MTR_4g117780 55.357 112 47 2 22 130 256 367 1.04e-31 115
MS.gene019504.t1 MTR_4g117770 55.357 112 47 2 22 130 256 367 1.28e-31 115
MS.gene019504.t1 MTR_4g117810 55.357 112 47 2 22 130 256 367 1.28e-31 115
MS.gene019504.t1 MTR_4g023190 52.206 136 54 4 1 128 225 357 1.38e-31 115
MS.gene019504.t1 MTR_5g023000 59.596 99 37 2 35 130 1 99 9.52e-31 106
MS.gene019504.t1 MTR_4g023150 50.000 136 57 4 1 128 226 358 1.64e-29 109
MS.gene019504.t1 MTR_4g023200 49.265 136 58 4 1 128 226 358 6.59e-29 107
MS.gene019504.t1 MTR_4g122860 50.000 128 54 4 10 130 196 320 2.90e-28 105
MS.gene019504.t1 MTR_5g070088 47.407 135 66 4 1 130 252 386 5.11e-28 105
MS.gene019504.t1 MTR_4g116330 53.097 113 48 2 23 130 229 341 7.64e-28 104
MS.gene019504.t1 MTR_4g117530 49.153 118 56 3 14 130 139 253 3.37e-27 101
MS.gene019504.t1 MTR_4g023440 61.957 92 32 2 41 130 242 332 3.60e-27 102
MS.gene019504.t1 MTR_4g121580 50.847 118 49 3 4 120 230 339 3.87e-27 103
MS.gene019504.t1 MTR_7g079400 46.377 138 62 5 2 130 203 337 1.03e-26 101
MS.gene019504.t1 MTR_8g102520 44.030 134 67 4 2 130 192 322 4.27e-25 97.4
MS.gene019504.t1 MTR_4g118400 49.219 128 44 6 2 124 183 294 7.04e-24 93.6
MS.gene019504.t1 MTR_7g079450 41.912 136 63 5 2 130 11 137 2.43e-23 88.6
MS.gene019504.t1 MTR_7g079370 44.882 127 63 4 2 124 227 350 3.50e-21 87.4
MS.gene019504.t1 MTR_8g098830 43.200 125 63 5 2 124 97 215 3.66e-21 85.1
MS.gene019504.t1 MTR_4g023430 46.667 105 48 3 33 130 119 222 9.27e-21 84.0
MS.gene019504.t1 MTR_7g117720 56.322 87 29 4 46 124 186 271 8.12e-18 77.0
MS.gene019504.t1 MTR_4g114700 50.000 88 38 3 40 121 131 218 1.47e-17 75.9
MS.gene019504.t1 MTR_4g122840 41.085 129 39 4 5 130 170 264 1.66e-17 76.3
MS.gene019504.t1 MTR_7g079420 37.500 136 72 6 2 129 1013 1143 1.36e-15 72.4
MS.gene019504.t1 MTR_4g023100 52.941 68 29 1 63 130 55 119 3.42e-13 62.0
MS.gene019504.t1 MTR_8g013005 59.649 57 21 1 76 130 3 59 5.72e-13 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCATGATCCTAAGTATTA+TGG 0.203715 8.1:-69742968 MS.gene019504:CDS
TATTATGGTTTGTGGGTATT+TGG 0.206725 8.1:-69742953 MS.gene019504:CDS
ATAATCCGGCACTTGAAATA+TGG 0.211797 8.1:-69742898 MS.gene019504:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.288501 8.1:-69742934 MS.gene019504:CDS
ATGGCCACTTCAAATGCCTT+TGG 0.385262 8.1:+69742689 None:intergenic
CAAGGTATGTCATTGTCAAT+AGG 0.400804 8.1:+69742824 None:intergenic
GGATCATGGTTGAAATCATC+TGG 0.411154 8.1:+69742980 None:intergenic
ATCCTAAGTATTATGGTTTG+TGG 0.419854 8.1:-69742961 MS.gene019504:CDS
TAATCCGGCACTTGAAATAT+GGG 0.448185 8.1:-69742897 MS.gene019504:CDS
ATCACCCATATTTCAAGTGC+CGG 0.473975 8.1:+69742893 None:intergenic
AGTGGTGATATTATTGGAAC+TGG 0.479168 8.1:-69742776 MS.gene019504:CDS
AATACAAACTGAAATCATCT+TGG 0.480922 8.1:-69742865 MS.gene019504:CDS
TCCTAAGTATTATGGTTTGT+GGG 0.484785 8.1:-69742960 MS.gene019504:CDS
AACCATAATACTTAGGATCA+TGG 0.502897 8.1:+69742966 None:intergenic
CTATTGACAATGACATACCT+TGG 0.516935 8.1:-69742823 MS.gene019504:CDS
TTGTCTTTGATTTAATGGAA+AGG 0.526787 8.1:-69743024 None:intergenic
AATAGGAATTACAAGAGTCT+TGG 0.534099 8.1:+69742841 None:intergenic
ATTGGAACTGGTGGTCGTCA+TGG 0.541567 8.1:-69742764 MS.gene019504:CDS
AACTGGTGGTCGTCATGGAT+TGG 0.553570 8.1:-69742759 MS.gene019504:CDS
ACCCACAAACCATAATACTT+AGG 0.570029 8.1:+69742959 None:intergenic
TGGTTGAAATCATCTGGAAG+AGG 0.588065 8.1:+69742986 None:intergenic
CATCTTGTTAAACTTGCTCA+CGG 0.601761 8.1:+69742633 None:intergenic
AAGCAGAGACTCTTTATACA+TGG 0.605588 8.1:+69742670 None:intergenic
GAATCCAAAGGCATTTGAAG+TGG 0.606386 8.1:-69742693 MS.gene019504:CDS
GCATCTCAAAATGAATCCAA+AGG 0.611920 8.1:-69742705 MS.gene019504:CDS
TAAACTTGCTCACGGTCACG+AGG 0.626552 8.1:+69742641 None:intergenic
TTGGTGAAGTATAATAACAA+AGG 0.635362 8.1:-69742740 MS.gene019504:CDS
GGTGATATTATTGGAACTGG+TGG 0.680138 8.1:-69742773 MS.gene019504:CDS
AAACTTGCTCACGGTCACGA+GGG 0.700858 8.1:+69742642 None:intergenic
GAGTATATAGGGGAAAACCA+AGG 0.732405 8.1:+69742806 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATATGAATTTTCATAATC+CGG - chr8.1:69742738-69742757 MS.gene019504:CDS 20.0%
! AATACAAACTGAAATCATCT+TGG - chr8.1:69742785-69742804 MS.gene019504:CDS 25.0%
! ACAAAAAGTGGTGATATTAT+TGG - chr8.1:69742868-69742887 MS.gene019504:CDS 25.0%
! TTGGTGAAGTATAATAACAA+AGG - chr8.1:69742910-69742929 MS.gene019504:CDS 25.0%
!! ACTTTTTGTAGAGTATATAG+GGG + chr8.1:69742857-69742876 None:intergenic 25.0%
!!! CACTTTTTGTAGAGTATATA+GGG + chr8.1:69742858-69742877 None:intergenic 25.0%
AACCATAATACTTAGGATCA+TGG + chr8.1:69742687-69742706 None:intergenic 30.0%
AACCATGATCCTAAGTATTA+TGG - chr8.1:69742682-69742701 MS.gene019504:CDS 30.0%
AATAGGAATTACAAGAGTCT+TGG + chr8.1:69742812-69742831 None:intergenic 30.0%
ATCCTAAGTATTATGGTTTG+TGG - chr8.1:69742689-69742708 MS.gene019504:CDS 30.0%
CCTATATACTCTACAAAAAG+TGG - chr8.1:69742856-69742875 MS.gene019504:CDS 30.0%
! TATTATGGTTTGTGGGTATT+TGG - chr8.1:69742697-69742716 MS.gene019504:CDS 30.0%
! TCCTAAGTATTATGGTTTGT+GGG - chr8.1:69742690-69742709 MS.gene019504:CDS 30.0%
!!! CCACTTTTTGTAGAGTATAT+AGG + chr8.1:69742859-69742878 None:intergenic 30.0%
AAGCAGAGACTCTTTATACA+TGG + chr8.1:69742983-69743002 None:intergenic 35.0%
ACCCACAAACCATAATACTT+AGG + chr8.1:69742694-69742713 None:intergenic 35.0%
AGTGGTGATATTATTGGAAC+TGG - chr8.1:69742874-69742893 MS.gene019504:CDS 35.0%
ATAATCCGGCACTTGAAATA+TGG - chr8.1:69742752-69742771 MS.gene019504:CDS 35.0%
CAAGGTATGTCATTGTCAAT+AGG + chr8.1:69742829-69742848 None:intergenic 35.0%
CTATTGACAATGACATACCT+TGG - chr8.1:69742827-69742846 MS.gene019504:CDS 35.0%
GCATCTCAAAATGAATCCAA+AGG - chr8.1:69742945-69742964 MS.gene019504:CDS 35.0%
TAATCCGGCACTTGAAATAT+GGG - chr8.1:69742753-69742772 MS.gene019504:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr8.1:69742716-69742735 MS.gene019504:CDS 35.0%
GAGTATATAGGGGAAAACCA+AGG + chr8.1:69742847-69742866 None:intergenic 40.0%
GGATCATGGTTGAAATCATC+TGG + chr8.1:69742673-69742692 None:intergenic 40.0%
GGTGATATTATTGGAACTGG+TGG - chr8.1:69742877-69742896 MS.gene019504:CDS 40.0%
TGGTTGAAATCATCTGGAAG+AGG + chr8.1:69742667-69742686 None:intergenic 40.0%
! ATCACCCATATTTCAAGTGC+CGG + chr8.1:69742760-69742779 None:intergenic 40.0%
!! GAATCCAAAGGCATTTGAAG+TGG - chr8.1:69742957-69742976 MS.gene019504:CDS 40.0%
ATGGCCACTTCAAATGCCTT+TGG + chr8.1:69742964-69742983 None:intergenic 45.0%
AACTGGTGGTCGTCATGGAT+TGG - chr8.1:69742891-69742910 MS.gene019504:CDS 50.0%
ATTGGAACTGGTGGTCGTCA+TGG - chr8.1:69742886-69742905 MS.gene019504:CDS 50.0%
! AAACTTGCTCACGGTCACGA+GGG + chr8.1:69743011-69743030 None:intergenic 50.0%
! TAAACTTGCTCACGGTCACG+AGG + chr8.1:69743012-69743031 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 69742640 69743032 69742640 ID=MS.gene019504
chr8.1 mRNA 69742640 69743032 69742640 ID=MS.gene019504.t1;Parent=MS.gene019504
chr8.1 exon 69742640 69743032 69742640 ID=MS.gene019504.t1.exon1;Parent=MS.gene019504.t1
chr8.1 CDS 69742640 69743032 69742640 ID=cds.MS.gene019504.t1;Parent=MS.gene019504.t1
Gene Sequence

>MS.gene019504

ATGGAAAGGAAACTGTTAGAGATACCTCTTCCAGATGATTTCAACCATGATCCTAAGTATTATGGTTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGAATATGAATTTTCATAATCCGGCACTTGAAATATGGGTGATGAAAGAATACAAACTGAAATCATCTTGGACCAAGACTCTTGTAATTCCTATTGACAATGACATACCTTGGTTTTCCCCTATATACTCTACAAAAAGTGGTGATATTATTGGAACTGGTGGTCGTCATGGATTGGTGAAGTATAATAACAAAGGACAACTTTTAGAGCATCTCAAAATGAATCCAAAGGCATTTGAAGTGGCCATGTATAAAGAGTCTCTGCTTTCACTCCCTCGTGACCGTGAGCAAGTTTAA

Protein sequence

>MS.gene019504.t1

MERKLLEIPLPDDFNHDPKYYGLWVFGEFLSLWNMNFHNPALEIWVMKEYKLKSSWTKTLVIPIDNDIPWFSPIYSTKSGDIIGTGGRHGLVKYNNKGQLLEHLKMNPKAFEVAMYKESLLSLPRDREQV