AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57532


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57532.t1 MTR_8g433040 71.654 127 32 2 2 124 238 364 1.31e-55 177
MS.gene57532.t1 MTR_8g032380 71.654 127 32 2 2 124 238 364 1.31e-55 177
MS.gene57532.t1 MTR_8g433030 68.293 123 34 2 2 119 215 337 2.48e-49 160
MS.gene57532.t1 MTR_8g032390 68.293 123 34 2 2 119 248 370 5.86e-49 160
MS.gene57532.t1 MTR_3g103020 57.692 130 48 2 2 124 249 378 8.01e-42 142
MS.gene57532.t1 MTR_4g116330 56.818 132 48 2 2 124 249 380 8.71e-41 139
MS.gene57532.t1 MTR_4g109270 56.154 130 50 2 2 124 235 364 5.59e-40 137
MS.gene57532.t1 MTR_8g040935 54.545 132 51 2 2 124 77 208 1.20e-39 132
MS.gene57532.t1 MTR_6g060650 55.039 129 49 3 2 123 246 372 2.91e-39 135
MS.gene57532.t1 MTR_4g117870 56.250 128 49 3 3 124 141 267 5.01e-39 132
MS.gene57532.t1 MTR_6g463430 54.762 126 54 2 2 124 77 202 1.11e-38 129
MS.gene57532.t1 MTR_4g023130 56.693 127 49 2 2 123 187 312 2.55e-38 131
MS.gene57532.t1 MTR_4g094365 57.895 133 44 4 2 124 234 364 9.54e-38 130
MS.gene57532.t1 MTR_4g021785 53.906 128 53 2 2 124 229 355 3.56e-37 129
MS.gene57532.t1 MTR_4g023380 50.000 152 47 4 2 124 228 379 6.92e-37 129
MS.gene57532.t1 MTR_4g109280 54.545 132 48 4 2 124 231 359 7.64e-37 128
MS.gene57532.t1 MTR_4g122850 59.398 133 41 6 2 124 235 364 1.29e-36 128
MS.gene57532.t1 MTR_4g117720 54.412 136 47 5 2 124 245 378 2.15e-36 127
MS.gene57532.t1 MTR_4g023080 54.331 127 52 2 2 123 228 353 2.26e-36 127
MS.gene57532.t1 MTR_4g023070 49.640 139 52 2 3 123 231 369 1.06e-35 125
MS.gene57532.t1 MTR_4g116320 56.250 128 45 3 2 120 129 254 2.41e-35 122
MS.gene57532.t1 MTR_4g023120 48.571 140 54 2 3 124 231 370 2.49e-35 124
MS.gene57532.t1 MTR_3g034180 55.385 130 48 4 2 124 363 489 3.30e-35 126
MS.gene57532.t1 MTR_1g055175 49.612 129 57 3 2 123 73 200 6.11e-35 119
MS.gene57532.t1 MTR_5g097210 54.331 127 48 4 2 121 237 360 7.79e-35 123
MS.gene57532.t1 MTR_4g023090 49.306 144 52 4 2 124 243 386 1.58e-34 123
MS.gene57532.t1 MTR_4g118360 53.488 129 45 5 6 123 1 125 1.41e-33 114
MS.gene57532.t1 MTR_1g055165 49.231 130 58 3 2 124 190 318 2.06e-33 119
MS.gene57532.t1 MTR_4g117850 51.562 128 53 3 2 121 250 376 2.12e-33 119
MS.gene57532.t1 MTR_4g118370 52.000 125 53 3 2 121 135 257 3.46e-33 116
MS.gene57532.t1 MTR_4g122860 50.394 127 49 3 9 124 197 320 8.61e-32 115
MS.gene57532.t1 MTR_4g023190 54.135 133 46 5 2 122 228 357 8.85e-32 115
MS.gene57532.t1 MTR_4g117750 55.357 112 43 2 17 121 369 480 9.71e-32 117
MS.gene57532.t1 MTR_4g117750 52.294 109 45 3 20 123 252 358 5.52e-25 98.6
MS.gene57532.t1 MTR_5g023000 60.204 98 32 2 34 124 2 99 1.41e-31 108
MS.gene57532.t1 MTR_4g118380 56.364 110 32 3 2 97 248 355 5.33e-31 113
MS.gene57532.t1 MTR_4g117780 55.856 111 42 3 21 124 257 367 9.92e-31 112
MS.gene57532.t1 MTR_4g117770 55.856 111 42 3 21 124 257 367 1.40e-30 112
MS.gene57532.t1 MTR_4g117810 55.856 111 42 3 21 124 257 367 1.40e-30 112
MS.gene57532.t1 MTR_4g023150 51.880 133 49 5 2 122 229 358 7.82e-30 110
MS.gene57532.t1 MTR_1g055135 50.000 134 52 6 3 124 245 375 8.96e-30 110
MS.gene57532.t1 MTR_5g070130 50.758 132 56 4 2 124 246 377 1.05e-29 110
MS.gene57532.t1 MTR_5g070080 52.000 125 52 3 2 119 257 380 4.62e-29 108
MS.gene57532.t1 MTR_4g023200 51.128 133 50 5 2 122 229 358 5.88e-29 107
MS.gene57532.t1 MTR_3g034160 55.469 128 49 3 2 123 249 374 8.48e-29 107
MS.gene57532.t1 MTR_4g116330 53.982 113 43 2 21 124 229 341 1.11e-28 106
MS.gene57532.t1 MTR_3g034130 58.000 100 35 2 2 96 249 346 1.44e-28 107
MS.gene57532.t1 MTR_4g121580 50.877 114 51 2 2 114 230 339 3.43e-28 106
MS.gene57532.t1 MTR_4g023440 59.341 91 32 1 39 124 242 332 8.43e-28 104
MS.gene57532.t1 MTR_1g055195 50.000 128 51 6 2 120 351 474 7.81e-27 103
MS.gene57532.t1 MTR_4g118400 62.791 86 25 2 40 118 209 294 1.32e-26 100
MS.gene57532.t1 MTR_4g117530 47.458 118 54 3 12 124 139 253 2.27e-26 99.0
MS.gene57532.t1 MTR_7g079450 44.531 128 61 4 3 124 14 137 5.26e-25 92.4
MS.gene57532.t1 MTR_7g079400 46.212 132 57 4 5 124 208 337 3.10e-24 95.1
MS.gene57532.t1 MTR_5g070088 46.212 132 62 4 2 124 255 386 2.61e-23 93.2
MS.gene57532.t1 MTR_1g055250 46.087 115 54 3 2 111 142 253 3.41e-23 92.0
MS.gene57532.t1 MTR_8g102520 42.188 128 64 4 5 124 197 322 2.76e-21 87.0
MS.gene57532.t1 MTR_7g079370 46.281 121 56 4 5 118 232 350 5.45e-21 86.7
MS.gene57532.t1 MTR_8g098830 40.650 123 63 5 1 118 98 215 2.44e-19 80.1
MS.gene57532.t1 MTR_7g117720 55.172 87 28 3 44 119 186 272 4.26e-19 80.5
MS.gene57532.t1 MTR_4g023430 42.857 105 48 3 31 124 119 222 5.34e-18 76.6
MS.gene57532.t1 MTR_7g079420 40.458 131 61 6 1 119 1014 1139 9.89e-18 78.2
MS.gene57532.t1 MTR_4g114700 49.425 87 34 2 39 115 132 218 2.49e-16 72.4
MS.gene57532.t1 MTR_8g013005 57.895 57 22 1 70 124 3 59 1.16e-12 58.9
MS.gene57532.t1 MTR_4g023100 54.545 55 25 0 70 124 65 119 6.81e-12 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTGGACAATTGAAATA+TGG 0.213992 8.3:-62068037 MS.gene57532:CDS
TGTTCTGATTTGTGGGTATT+TGG 0.261443 8.3:-62068092 MS.gene57532:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.288501 8.3:-62068073 MS.gene57532:CDS
TAATTGGACAATTGAAATAT+GGG 0.403350 8.3:-62068036 MS.gene57532:CDS
ATCTTCGGTGTTCTGATTTG+TGG 0.407497 8.3:-62068100 MS.gene57532:CDS
AGATCATGGTCAAAATCATC+TGG 0.426340 8.3:+62068119 None:intergenic
GAGTCTCTGCTTTCACTGCC+TGG 0.432512 8.3:-62067810 MS.gene57532:CDS
TGGTCAAAATCATCTGGAAA+TGG 0.450872 8.3:+62068125 None:intergenic
AATTGAAATATGGGTGATGA+AGG 0.467515 8.3:-62068027 MS.gene57532:CDS
TCTTCGGTGTTCTGATTTGT+GGG 0.470239 8.3:-62068099 MS.gene57532:CDS
GAATCTGAATGCATCTAAAG+TGG 0.499583 8.3:-62067844 MS.gene57532:CDS
ACTGCCTGGTGACCGTCAGC+AGG 0.501497 8.3:-62067796 MS.gene57532:CDS
AATACAAACAACATTCGTCT+TGG 0.503398 8.3:-62068004 MS.gene57532:CDS
TTGGTGAAGTATAACGATAA+AGG 0.509096 8.3:-62067891 MS.gene57532:CDS
AACTGACGGTGGTCGTGGAT+TGG 0.528254 8.3:-62067910 MS.gene57532:CDS
GTTGGAACTGACGGTGGTCG+TGG 0.542172 8.3:-62067915 MS.gene57532:CDS
ATTGGGGAAAAGTGAAGGCT+AGG 0.546198 8.3:+62067963 None:intergenic
TAACGATAAAGGACAGGAGA+TGG 0.548347 8.3:-62067880 MS.gene57532:CDS
GAAGTATAACGATAAAGGAC+AGG 0.548434 8.3:-62067886 MS.gene57532:CDS
AATCAGAACACCGAAGATCA+TGG 0.557498 8.3:+62068105 None:intergenic
AAGCAGAGACTCTGTATACA+TGG 0.569558 8.3:+62067821 None:intergenic
TAAACCTGCTGACGGTCACC+AGG 0.573679 8.3:+62067792 None:intergenic
AGTATATTGGGGAAAAGTGA+AGG 0.588482 8.3:+62067958 None:intergenic
GGTGATATTGTTGGAACTGA+CGG 0.665403 8.3:-62067924 MS.gene57532:CDS
CATCTTGTTAAACCTGCTGA+CGG 0.678381 8.3:+62067784 None:intergenic
GATATTGTTGGAACTGACGG+TGG 0.737520 8.3:-62067921 MS.gene57532:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTGGACAATTGAAATA+TGG - chr8.3:62067897-62067916 MS.gene57532:CDS 20.0%
!! TAATTGGACAATTGAAATAT+GGG - chr8.3:62067898-62067917 MS.gene57532:CDS 20.0%
!!! GGATTAGAAATTTTCATAAT+TGG - chr8.3:62067882-62067901 MS.gene57532:CDS 20.0%
!! ACTTTTTGTAGAGTATATTG+GGG + chr8.3:62067990-62068009 None:intergenic 25.0%
!!! CACTTTTTGTAGAGTATATT+GGG + chr8.3:62067991-62068010 None:intergenic 25.0%
AATACAAACAACATTCGTCT+TGG - chr8.3:62067930-62067949 MS.gene57532:CDS 30.0%
AATTGAAATATGGGTGATGA+AGG - chr8.3:62067907-62067926 MS.gene57532:CDS 30.0%
ACAAAAAGTGGTGATATTGT+TGG - chr8.3:62068001-62068020 MS.gene57532:CDS 30.0%
CCAATATACTCTACAAAAAG+TGG - chr8.3:62067989-62068008 MS.gene57532:CDS 30.0%
TTGGTGAAGTATAACGATAA+AGG - chr8.3:62068043-62068062 MS.gene57532:CDS 30.0%
!! ATGATTTTGACCATGATCTT+CGG - chr8.3:62067819-62067838 MS.gene57532:CDS 30.0%
!!! CCACTTTTTGTAGAGTATAT+TGG + chr8.3:62067992-62068011 None:intergenic 30.0%
AGATCATGGTCAAAATCATC+TGG + chr8.3:62067818-62067837 None:intergenic 35.0%
AGTATATTGGGGAAAAGTGA+AGG + chr8.3:62067979-62067998 None:intergenic 35.0%
GAAGTATAACGATAAAGGAC+AGG - chr8.3:62068048-62068067 MS.gene57532:CDS 35.0%
GAATCTGAATGCATCTAAAG+TGG - chr8.3:62068090-62068109 MS.gene57532:CDS 35.0%
TGGTCAAAATCATCTGGAAA+TGG + chr8.3:62067812-62067831 None:intergenic 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr8.3:62067861-62067880 MS.gene57532:CDS 35.0%
! TGTTCTGATTTGTGGGTATT+TGG - chr8.3:62067842-62067861 MS.gene57532:CDS 35.0%
AAGCAGAGACTCTGTATACA+TGG + chr8.3:62068116-62068135 None:intergenic 40.0%
AATCAGAACACCGAAGATCA+TGG + chr8.3:62067832-62067851 None:intergenic 40.0%
GGTGATATTGTTGGAACTGA+CGG - chr8.3:62068010-62068029 MS.gene57532:CDS 40.0%
TAACGATAAAGGACAGGAGA+TGG - chr8.3:62068054-62068073 MS.gene57532:CDS 40.0%
!! ATCTTCGGTGTTCTGATTTG+TGG - chr8.3:62067834-62067853 MS.gene57532:CDS 40.0%
!! TCTTCGGTGTTCTGATTTGT+GGG - chr8.3:62067835-62067854 MS.gene57532:CDS 40.0%
ATTGGGGAAAAGTGAAGGCT+AGG + chr8.3:62067974-62067993 None:intergenic 45.0%
GATATTGTTGGAACTGACGG+TGG - chr8.3:62068013-62068032 MS.gene57532:CDS 45.0%
AACTGACGGTGGTCGTGGAT+TGG - chr8.3:62068024-62068043 MS.gene57532:CDS 55.0%
GAGTCTCTGCTTTCACTGCC+TGG - chr8.3:62068124-62068143 MS.gene57532:CDS 55.0%
TAAACCTGCTGACGGTCACC+AGG + chr8.3:62068145-62068164 None:intergenic 55.0%
GTTGGAACTGACGGTGGTCG+TGG - chr8.3:62068019-62068038 MS.gene57532:CDS 60.0%
ACTGCCTGGTGACCGTCAGC+AGG - chr8.3:62068138-62068157 MS.gene57532:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 62067791 62068165 62067791 ID=MS.gene57532
chr8.3 mRNA 62067791 62068165 62067791 ID=MS.gene57532.t1;Parent=MS.gene57532
chr8.3 exon 62067791 62068165 62067791 ID=MS.gene57532.t1.exon1;Parent=MS.gene57532.t1
chr8.3 CDS 62067791 62068165 62067791 ID=cds.MS.gene57532.t1;Parent=MS.gene57532.t1
Gene Sequence

>MS.gene57532

ATGAAACTGTTACAGATACCATTTCCAGATGATTTTGACCATGATCTTCGGTGTTCTGATTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGATTAGAAATTTTCATAATTGGACAATTGAAATATGGGTGATGAAGGAATACAAACAACATTCGTCTTGGACTAAGTCTCTTGTAATTCCTAGCCTTCACTTTTCCCCAATATACTCTACAAAAAGTGGTGATATTGTTGGAACTGACGGTGGTCGTGGATTGGTGAAGTATAACGATAAAGGACAGGAGATGGAGTATCTCGAAATGAATCTGAATGCATCTAAAGTGGCCATGTATACAGAGTCTCTGCTTTCACTGCCTGGTGACCGTCAGCAGGTTTAA

Protein sequence

>MS.gene57532.t1

MKLLQIPFPDDFDHDLRCSDLWVFGEFLSLWIRNFHNWTIEIWVMKEYKQHSSWTKSLVIPSLHFSPIYSTKSGDIVGTDGGRGLVKYNDKGQEMEYLEMNLNASKVAMYTESLLSLPGDRQQV