AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57717


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57717.t1 MTR_3g103020 59.346 214 79 4 1 211 170 378 1.53e-72 224
MS.gene57717.t1 MTR_4g109270 58.416 202 81 2 11 211 165 364 3.75e-72 223
MS.gene57717.t1 MTR_4g109280 59.898 197 72 4 17 211 168 359 5.13e-72 223
MS.gene57717.t1 MTR_4g117720 60.092 218 73 8 1 211 168 378 2.97e-70 219
MS.gene57717.t1 MTR_4g094365 58.000 200 78 3 14 211 169 364 3.77e-70 218
MS.gene57717.t1 MTR_4g116320 58.586 198 71 4 15 208 65 255 2.62e-69 213
MS.gene57717.t1 MTR_4g117850 55.769 208 86 3 1 204 168 373 2.12e-68 214
MS.gene57717.t1 MTR_6g060650 58.462 195 76 3 16 210 183 372 2.76e-68 213
MS.gene57717.t1 MTR_8g040935 54.717 212 88 4 4 211 1 208 7.07e-68 207
MS.gene57717.t1 MTR_8g433030 57.416 209 78 6 1 206 137 337 7.22e-68 211
MS.gene57717.t1 MTR_8g032390 57.416 209 78 6 1 206 170 370 2.96e-67 211
MS.gene57717.t1 MTR_4g023130 56.075 214 80 6 4 210 106 312 3.27e-67 209
MS.gene57717.t1 MTR_4g116330 56.345 197 77 4 19 211 189 380 1.20e-65 207
MS.gene57717.t1 MTR_4g122850 54.884 215 87 6 1 211 156 364 2.28e-65 206
MS.gene57717.t1 MTR_4g117770 56.000 200 83 2 15 211 170 367 3.27e-65 205
MS.gene57717.t1 MTR_4g117810 56.000 200 83 2 15 211 170 367 3.27e-65 205
MS.gene57717.t1 MTR_4g023080 54.717 212 82 6 6 210 149 353 6.09e-65 204
MS.gene57717.t1 MTR_4g117530 54.639 194 52 4 18 211 96 253 2.66e-63 197
MS.gene57717.t1 MTR_6g463430 50.239 209 96 2 4 211 1 202 2.79e-63 195
MS.gene57717.t1 MTR_4g117780 54.000 200 87 2 15 211 170 367 3.99e-63 200
MS.gene57717.t1 MTR_3g034180 53.670 218 88 5 1 210 276 488 6.02e-61 197
MS.gene57717.t1 MTR_4g023190 53.488 215 85 7 4 209 149 357 1.03e-60 194
MS.gene57717.t1 MTR_8g433040 54.680 203 75 5 11 211 177 364 1.10e-59 191
MS.gene57717.t1 MTR_8g032380 54.680 203 75 5 11 211 177 364 1.10e-59 191
MS.gene57717.t1 MTR_4g118400 57.368 190 64 5 16 205 122 294 2.36e-59 188
MS.gene57717.t1 MTR_4g023380 49.130 230 82 7 6 211 161 379 4.38e-58 187
MS.gene57717.t1 MTR_4g021785 50.000 212 95 4 6 211 149 355 6.55e-58 186
MS.gene57717.t1 MTR_4g023070 49.099 222 97 5 1 210 152 369 1.77e-57 186
MS.gene57717.t1 MTR_1g055175 48.611 216 91 7 1 211 1 201 6.97e-57 179
MS.gene57717.t1 MTR_4g023090 48.430 223 91 6 1 209 170 382 2.89e-56 183
MS.gene57717.t1 MTR_4g023120 49.327 223 97 6 1 211 152 370 1.01e-55 181
MS.gene57717.t1 MTR_4g023150 52.093 215 85 8 6 209 151 358 1.73e-55 180
MS.gene57717.t1 MTR_4g118380 53.886 193 73 5 1 184 172 357 3.11e-55 179
MS.gene57717.t1 MTR_3g034160 55.446 202 79 4 15 210 178 374 1.28e-54 178
MS.gene57717.t1 MTR_4g023200 50.230 217 90 8 4 209 149 358 2.62e-54 177
MS.gene57717.t1 MTR_3g034130 50.244 205 90 4 1 197 162 362 9.99e-53 173
MS.gene57717.t1 MTR_4g117750 48.182 220 98 6 1 210 145 358 8.37e-52 174
MS.gene57717.t1 MTR_4g117750 59.459 111 43 1 99 209 373 481 4.04e-33 124
MS.gene57717.t1 MTR_4g118370 47.393 211 85 5 1 208 70 257 1.24e-51 167
MS.gene57717.t1 MTR_5g097210 49.282 209 99 4 1 207 156 359 3.56e-50 167
MS.gene57717.t1 MTR_1g055165 52.542 177 76 5 37 211 148 318 2.52e-49 163
MS.gene57717.t1 MTR_5g070080 49.763 211 96 7 4 211 183 386 1.81e-48 163
MS.gene57717.t1 MTR_4g117870 46.445 211 72 5 1 211 98 267 6.45e-48 158
MS.gene57717.t1 MTR_1g055195 48.529 204 88 6 18 211 282 478 9.74e-48 163
MS.gene57717.t1 MTR_4g023440 46.226 212 65 7 1 211 169 332 6.55e-47 157
MS.gene57717.t1 MTR_1g055135 43.636 220 109 7 1 211 162 375 1.09e-44 152
MS.gene57717.t1 MTR_5g070088 45.283 212 103 4 1 206 176 380 1.22e-44 153
MS.gene57717.t1 MTR_4g116330 45.685 197 59 6 19 211 189 341 5.16e-42 145
MS.gene57717.t1 MTR_5g070130 43.333 210 96 5 17 211 176 377 6.12e-39 138
MS.gene57717.t1 MTR_8g102520 40.455 220 112 8 1 211 113 322 1.73e-38 135
MS.gene57717.t1 MTR_7g079400 44.340 212 94 11 14 211 136 337 1.17e-37 133
MS.gene57717.t1 MTR_5g023000 66.000 100 32 2 112 211 2 99 4.68e-37 125
MS.gene57717.t1 MTR_4g122860 42.268 194 76 7 24 211 157 320 2.42e-36 130
MS.gene57717.t1 MTR_1g055250 43.229 192 89 8 15 198 74 253 2.88e-35 127
MS.gene57717.t1 MTR_4g117430 59.000 100 39 1 11 110 57 154 1.25e-34 120
MS.gene57717.t1 MTR_7g079370 42.512 207 100 8 6 205 156 350 5.30e-34 124
MS.gene57717.t1 MTR_4g118360 58.261 115 40 3 99 211 18 126 1.44e-32 114
MS.gene57717.t1 MTR_8g098830 42.775 173 83 7 15 180 31 194 2.56e-32 117
MS.gene57717.t1 MTR_4g114700 40.526 190 59 4 17 202 79 218 1.77e-31 115
MS.gene57717.t1 MTR_4g121580 44.211 190 69 7 16 201 183 339 1.50e-30 115
MS.gene57717.t1 MTR_2g048580 36.239 218 115 8 3 211 140 342 6.49e-30 113
MS.gene57717.t1 MTR_7g079450 45.833 144 67 5 70 211 3 137 1.73e-29 107
MS.gene57717.t1 MTR_7g079420 38.830 188 96 7 34 211 966 1144 2.37e-25 103
MS.gene57717.t1 MTR_8g013010 49.533 107 49 2 2 108 208 309 1.80e-24 98.6
MS.gene57717.t1 MTR_8g013005 71.186 59 17 0 153 211 1 59 7.87e-21 82.4
MS.gene57717.t1 MTR_7g117720 57.778 90 32 3 122 207 186 273 3.05e-19 83.6
MS.gene57717.t1 MTR_4g122840 40.580 138 44 3 75 211 164 264 3.62e-19 83.2
MS.gene57717.t1 MTR_4g023430 39.860 143 64 5 79 211 92 222 1.26e-16 75.5
MS.gene57717.t1 MTR_4g116340 50.685 73 35 1 2 74 176 247 2.01e-16 75.5
MS.gene57717.t1 MTR_6g034720 46.835 79 41 1 1 79 86 163 1.32e-15 72.8
MS.gene57717.t1 MTR_4g109290 66.038 53 18 0 159 211 136 188 8.88e-14 67.4
MS.gene57717.t1 MTR_1g094210 30.769 169 96 8 20 180 221 376 1.51e-13 68.9
MS.gene57717.t1 MTR_1g094210 30.675 163 92 8 20 174 221 370 1.43e-12 65.9
MS.gene57717.t1 MTR_0097s0030 27.879 165 104 6 10 162 174 335 3.18e-12 65.1
MS.gene57717.t1 MTR_2g047240 27.879 165 104 6 10 162 174 335 3.18e-12 65.1
MS.gene57717.t1 MTR_6g088385 27.014 211 126 8 17 205 116 320 3.21e-12 64.7
MS.gene57717.t1 MTR_0667s0010 30.065 153 88 7 18 159 183 327 3.42e-12 65.1
MS.gene57717.t1 MTR_8g099460 27.950 161 109 4 1 159 167 322 1.01e-11 63.5
MS.gene57717.t1 MTR_2g064960 69.048 42 13 0 170 211 18 59 1.08e-11 58.5
MS.gene57717.t1 MTR_4g023100 60.000 55 20 1 156 210 66 118 1.95e-11 59.3
MS.gene57717.t1 MTR_3g010970 28.191 188 113 7 23 203 219 391 2.54e-11 62.4
MS.gene57717.t1 MTR_7g055830 26.786 224 130 7 17 207 138 360 3.09e-11 62.0
MS.gene57717.t1 MTR_1g055145 48.649 74 36 2 52 125 131 202 3.65e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57717.t1 AT3G06240 28.750 160 99 5 6 160 225 374 8.68e-11 60.8

Find 41 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCTGGTTTGTGGGTATT+TGG 0.119331 8.3:+8794683 MS.gene57717:CDS
ATTCTGGTTTGTGGGTATTT+GGG 0.224908 8.3:+8794684 MS.gene57717:CDS
TAATACATGGAAAGAAATTA+AGG 0.236785 8.3:+8794475 MS.gene57717:CDS
GACAATATACTTGACTATTC+TGG 0.288946 8.3:+8794668 MS.gene57717:CDS
ATAATCATACGGTTGAAATA+TGG 0.292170 8.3:+8794738 MS.gene57717:CDS
AGCTACTGTTTCATCACATT+TGG 0.319260 8.3:+8794433 MS.gene57717:CDS
TACAGAATGGGATTGCTATA+AGG 0.332225 8.3:-8794509 None:intergenic
GTTAAAGAGCAACCCTGCTT+TGG 0.343363 8.3:-8794537 None:intergenic
CTCATTAACATTGGCATCAA+TGG 0.363458 8.3:-8794813 None:intergenic
ACAGAATGGGATTGCTATAA+GGG 0.374494 8.3:-8794508 None:intergenic
TTAAAGAGCAACCCTGCTTT+GGG 0.384566 8.3:-8794536 None:intergenic
ATTGGAACATGTGGTGGTAT+TGG 0.396640 8.3:+8794881 MS.gene57717:CDS
TACTTGACTATTCTGGTTTG+TGG 0.398497 8.3:+8794675 MS.gene57717:CDS
TAATCATACGGTTGAAATAT+GGG 0.438296 8.3:+8794739 MS.gene57717:CDS
AATACAAAGTGCATTCATCT+TGG 0.440860 8.3:+8794771 MS.gene57717:CDS
AGTATATTGTCAAACTCATC+TGG 0.441674 8.3:-8794656 None:intergenic
ACTTGACTATTCTGGTTTGT+GGG 0.445951 8.3:+8794676 MS.gene57717:CDS
TTCTGGTTTGTGGGTATTTG+GGG 0.446634 8.3:+8794685 MS.gene57717:CDS
TTGCCTTTGATTTAATGAAA+AGG 0.452967 8.3:+8794612 MS.gene57717:CDS
TAACGAGGCTATTCATTGGT+TGG 0.473376 8.3:+8794556 MS.gene57717:CDS
TGCTTTGGGCTGATACAGAA+TGG 0.489790 8.3:-8794522 None:intergenic
TCTCGTTGAAAGATAATACA+TGG 0.498466 8.3:+8794462 MS.gene57717:CDS
TCTTTAACGAGGCTATTCAT+TGG 0.501818 8.3:+8794552 MS.gene57717:CDS
ATTCTGTATCAGCCCAAAGC+AGG 0.503682 8.3:+8794524 MS.gene57717:CDS
AATACATGGAAAGAAATTAA+GGG 0.504563 8.3:+8794476 MS.gene57717:CDS
GAGTCTCTGCTCTCACTCCC+TGG 0.513546 8.3:+8794992 MS.gene57717:CDS
GCTTTGGGCTGATACAGAAT+GGG 0.513590 8.3:-8794521 None:intergenic
GGGATTCACGTGAATCGTTA+CGG 0.517791 8.3:-8794952 None:intergenic
ATGTGATGAAACAGTAGCTA+AGG 0.533173 8.3:-8794429 None:intergenic
TAAGCTTGTTCACTTTCACC+AGG 0.543687 8.3:-8795010 None:intergenic
CTCTGTATACATAGCCACTT+GGG 0.546267 8.3:-8794972 None:intergenic
AGAAAGAGACTCATTAACAT+TGG 0.571014 8.3:-8794822 None:intergenic
AACATGTGGTGGTATTGGAT+TGG 0.578026 8.3:+8794886 MS.gene57717:CDS
AAGCTTGTTCACTTTCACCA+GGG 0.593323 8.3:-8795009 None:intergenic
TTCTGTATCAGCCCAAAGCA+GGG 0.611541 8.3:+8794525 MS.gene57717:CDS
ACTCTGTATACATAGCCACT+TGG 0.613352 8.3:-8794973 None:intergenic
AGCAGGGTTGCTCTTTAACG+AGG 0.621468 8.3:+8794541 MS.gene57717:CDS
GGTGATATTATTGGAACATG+TGG 0.633288 8.3:+8794872 MS.gene57717:CDS
GATATTATTGGAACATGTGG+TGG 0.648140 8.3:+8794875 MS.gene57717:CDS
TTGGTGAAGTACAATGACAA+AGG 0.668246 8.3:+8794905 MS.gene57717:CDS
CGATTCACGTGAATCCCAAG+TGG 0.676953 8.3:+8794958 MS.gene57717:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATAATTTTGATAATCATA+CGG + chr8.3:8794727-8794746 MS.gene57717:CDS 10.0%
!! AATACATGGAAAGAAATTAA+GGG + chr8.3:8794476-8794495 MS.gene57717:CDS 20.0%
!! TAATACATGGAAAGAAATTA+AGG + chr8.3:8794475-8794494 MS.gene57717:CDS 20.0%
!!! TTTCCTTTTCATTAAATCAA+AGG - chr8.3:8794618-8794637 None:intergenic 20.0%
! ATAATCATACGGTTGAAATA+TGG + chr8.3:8794738-8794757 MS.gene57717:CDS 25.0%
! TAATCATACGGTTGAAATAT+GGG + chr8.3:8794739-8794758 MS.gene57717:CDS 25.0%
!! TTGCCTTTGATTTAATGAAA+AGG + chr8.3:8794612-8794631 MS.gene57717:CDS 25.0%
AATACAAAGTGCATTCATCT+TGG + chr8.3:8794771-8794790 MS.gene57717:CDS 30.0%
AGAAAGAGACTCATTAACAT+TGG - chr8.3:8794825-8794844 None:intergenic 30.0%
AGTATATTGTCAAACTCATC+TGG - chr8.3:8794659-8794678 None:intergenic 30.0%
GACAATATACTTGACTATTC+TGG + chr8.3:8794668-8794687 MS.gene57717:CDS 30.0%
GCAAAAAGTGGTGATATTAT+TGG + chr8.3:8794863-8794882 MS.gene57717:CDS 30.0%
TCTCGTTGAAAGATAATACA+TGG + chr8.3:8794462-8794481 MS.gene57717:CDS 30.0%
ACAGAATGGGATTGCTATAA+GGG - chr8.3:8794511-8794530 None:intergenic 35.0%
AGCTACTGTTTCATCACATT+TGG + chr8.3:8794433-8794452 MS.gene57717:CDS 35.0%
ATGTGATGAAACAGTAGCTA+AGG - chr8.3:8794432-8794451 None:intergenic 35.0%
CTCATTAACATTGGCATCAA+TGG - chr8.3:8794816-8794835 None:intergenic 35.0%
GATATTATTGGAACATGTGG+TGG + chr8.3:8794875-8794894 MS.gene57717:CDS 35.0%
GGTGATATTATTGGAACATG+TGG + chr8.3:8794872-8794891 MS.gene57717:CDS 35.0%
TACAGAATGGGATTGCTATA+AGG - chr8.3:8794512-8794531 None:intergenic 35.0%
TCTTTAACGAGGCTATTCAT+TGG + chr8.3:8794552-8794571 MS.gene57717:CDS 35.0%
TTGGTGAAGTACAATGACAA+AGG + chr8.3:8794905-8794924 MS.gene57717:CDS 35.0%
! TAAGGTTTTACTTGCATCGT+AGG - chr8.3:8794414-8794433 None:intergenic 35.0%
!! ACTTGACTATTCTGGTTTGT+GGG + chr8.3:8794676-8794695 MS.gene57717:CDS 35.0%
!! ATTCTGGTTTGTGGGTATTT+GGG + chr8.3:8794684-8794703 MS.gene57717:CDS 35.0%
!! TACTTGACTATTCTGGTTTG+TGG + chr8.3:8794675-8794694 MS.gene57717:CDS 35.0%
!! TATTCTGGTTTGTGGGTATT+TGG + chr8.3:8794683-8794702 MS.gene57717:CDS 35.0%
ACTCTGTATACATAGCCACT+TGG - chr8.3:8794976-8794995 None:intergenic 40.0%
ATTGGAACATGTGGTGGTAT+TGG + chr8.3:8794881-8794900 MS.gene57717:CDS 40.0%
CTCTGTATACATAGCCACTT+GGG - chr8.3:8794975-8794994 None:intergenic 40.0%
TAACGAGGCTATTCATTGGT+TGG + chr8.3:8794556-8794575 MS.gene57717:CDS 40.0%
TTAAAGAGCAACCCTGCTTT+GGG - chr8.3:8794539-8794558 None:intergenic 40.0%
! AACATGTGGTGGTATTGGAT+TGG + chr8.3:8794886-8794905 MS.gene57717:CDS 40.0%
! AAGCTTGTTCACTTTCACCA+GGG - chr8.3:8795012-8795031 None:intergenic 40.0%
! ACTTTTTGCACAGCACAATG+GGG - chr8.3:8794852-8794871 None:intergenic 40.0%
! TAAGCTTGTTCACTTTCACC+AGG - chr8.3:8795013-8795032 None:intergenic 40.0%
!! CACTTTTTGCACAGCACAAT+GGG - chr8.3:8794853-8794872 None:intergenic 40.0%
!! TTCTGGTTTGTGGGTATTTG+GGG + chr8.3:8794685-8794704 MS.gene57717:CDS 40.0%
ATTCTGTATCAGCCCAAAGC+AGG + chr8.3:8794524-8794543 MS.gene57717:CDS 45.0%
CCATTGTGCTGTGCAAAAAG+TGG + chr8.3:8794851-8794870 MS.gene57717:CDS 45.0%
GGGATTCACGTGAATCGTTA+CGG - chr8.3:8794955-8794974 None:intergenic 45.0%
GTTAAAGAGCAACCCTGCTT+TGG - chr8.3:8794540-8794559 None:intergenic 45.0%
TTCTGTATCAGCCCAAAGCA+GGG + chr8.3:8794525-8794544 MS.gene57717:CDS 45.0%
! GCTTTGGGCTGATACAGAAT+GGG - chr8.3:8794524-8794543 None:intergenic 45.0%
! TGCTTTGGGCTGATACAGAA+TGG - chr8.3:8794525-8794544 None:intergenic 45.0%
!!! CCACTTTTTGCACAGCACAA+TGG - chr8.3:8794854-8794873 None:intergenic 45.0%
AGCAGGGTTGCTCTTTAACG+AGG + chr8.3:8794541-8794560 MS.gene57717:CDS 50.0%
CGATTCACGTGAATCCCAAG+TGG + chr8.3:8794958-8794977 MS.gene57717:CDS 50.0%
GAGTCTCTGCTCTCACTCCC+TGG + chr8.3:8794992-8795011 MS.gene57717:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 8794398 8795033 8794398 ID=MS.gene57717
chr8.3 mRNA 8794398 8795033 8794398 ID=MS.gene57717.t1;Parent=MS.gene57717
chr8.3 exon 8794398 8795033 8794398 ID=MS.gene57717.t1.exon1;Parent=MS.gene57717.t1
chr8.3 CDS 8794398 8795033 8794398 ID=cds.MS.gene57717.t1;Parent=MS.gene57717.t1
Gene Sequence

>MS.gene57717

ATGGTTTTAATGTCCTACGATGCAAGTAAAACCTTAGCTACTGTTTCATCACATTTGGAATTTTTCTCGTTGAAAGATAATACATGGAAAGAAATTAAGGGTACTCACTTCCCTTATAGCAATCCCATTCTGTATCAGCCCAAAGCAGGGTTGCTCTTTAACGAGGCTATTCATTGGTTGGCTTATCATAATGATTTGCATCAGTATGTTGTTGTTGCCTTTGATTTAATGAAAAGGAAACTTTGCAATATGTATTTGCCAGATGAGTTTGACAATATACTTGACTATTCTGGTTTGTGGGTATTTGGGGAATTTCTCAGTTTTTCGTCTAATAATTTTGATAATCATACGGTTGAAATATGGGTGATGAAAGAATACAAAGTGCATTCATCTTGGACAAAGACTCATGTTCTGTCCATTGATGCCAATGTTAATGAGTCTCTTTCTTTTTCCCCATTGTGCTGTGCAAAAAGTGGTGATATTATTGGAACATGTGGTGGTATTGGATTGGTGAAGTACAATGACAAAGGACAGCTACTAGAGCATTGCTCTTACCGTAACGATTCACGTGAATCCCAAGTGGCTATGTATACAGAGTCTCTGCTCTCACTCCCTGGTGAAAGTGAACAAGCTTAA

Protein sequence

>MS.gene57717.t1

MVLMSYDASKTLATVSSHLEFFSLKDNTWKEIKGTHFPYSNPILYQPKAGLLFNEAIHWLAYHNDLHQYVVVAFDLMKRKLCNMYLPDEFDNILDYSGLWVFGEFLSFSSNNFDNHTVEIWVMKEYKVHSSWTKTHVLSIDANVNESLSFSPLCCAKSGDIIGTCGGIGLVKYNDKGQLLEHCSYRNDSRESQVAMYTESLLSLPGESEQA