AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63658

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63658.t1 MTR_3g034180 88.179 313 36 1 1 313 178 489 0.0 569
MS.gene63658.t1 MTR_3g034130 90.635 299 28 0 1 299 64 362 0.0 565
MS.gene63658.t1 MTR_3g034160 87.179 312 39 1 1 312 64 374 0.0 539
MS.gene63658.t1 MTR_3g103020 63.694 314 99 6 3 312 75 377 2.53e-132 381
MS.gene63658.t1 MTR_4g117720 61.180 322 101 10 1 312 70 377 1.86e-124 361
MS.gene63658.t1 MTR_6g060650 60.559 322 106 6 1 312 62 372 3.55e-120 350
MS.gene63658.t1 MTR_4g109270 57.862 318 110 8 1 312 64 363 6.14e-111 326
MS.gene63658.t1 MTR_4g122850 55.140 321 127 9 2 316 58 367 1.70e-110 326
MS.gene63658.t1 MTR_4g109280 60.317 315 106 8 1 312 60 358 9.07e-108 318
MS.gene63658.t1 MTR_4g117850 54.516 310 117 9 7 310 85 376 4.01e-99 296
MS.gene63658.t1 MTR_4g117770 54.921 315 114 7 9 313 71 367 5.09e-99 296
MS.gene63658.t1 MTR_4g117810 54.921 315 114 7 9 313 71 367 5.09e-99 296
MS.gene63658.t1 MTR_4g094365 52.038 319 130 8 1 313 63 364 5.77e-97 291
MS.gene63658.t1 MTR_4g023080 53.503 314 120 8 1 312 64 353 1.55e-96 289
MS.gene63658.t1 MTR_4g118380 53.746 307 110 6 1 286 62 357 3.49e-96 288
MS.gene63658.t1 MTR_4g117780 51.393 323 126 8 1 313 66 367 1.18e-94 285
MS.gene63658.t1 MTR_4g023090 50.150 333 129 9 1 313 71 386 2.84e-94 285
MS.gene63658.t1 MTR_5g097210 49.838 309 139 6 3 310 67 360 1.44e-92 280
MS.gene63658.t1 MTR_4g021785 51.623 308 124 7 7 312 70 354 3.99e-91 275
MS.gene63658.t1 MTR_4g116330 51.780 309 128 7 10 312 86 379 4.35e-91 276
MS.gene63658.t1 MTR_4g117750 50.155 323 130 8 3 315 60 361 8.49e-90 276
MS.gene63658.t1 MTR_4g117750 56.637 113 47 1 205 315 373 485 3.99e-33 127
MS.gene63658.t1 MTR_5g070130 49.387 326 139 10 3 315 67 379 7.35e-89 271
MS.gene63658.t1 MTR_4g023380 48.657 335 112 9 7 313 77 379 2.21e-87 267
MS.gene63658.t1 MTR_4g023070 48.795 332 138 10 1 316 58 373 3.49e-87 266
MS.gene63658.t1 MTR_4g117870 49.682 314 110 7 1 313 1 267 1.15e-86 261
MS.gene63658.t1 MTR_8g433040 50.156 321 123 10 2 313 72 364 4.50e-86 263
MS.gene63658.t1 MTR_8g032380 50.156 321 123 10 2 313 72 364 4.50e-86 263
MS.gene63658.t1 MTR_5g070080 48.348 333 134 8 4 312 67 385 5.13e-86 263
MS.gene63658.t1 MTR_1g055135 48.089 314 143 8 7 312 73 374 2.51e-85 261
MS.gene63658.t1 MTR_4g023190 50.629 318 125 11 1 311 65 357 3.59e-83 255
MS.gene63658.t1 MTR_4g023120 48.328 329 138 11 1 313 58 370 3.30e-82 253
MS.gene63658.t1 MTR_4g023130 51.389 288 116 7 27 312 47 312 7.85e-82 250
MS.gene63658.t1 MTR_1g055195 48.525 305 126 7 20 316 200 481 3.31e-81 254
MS.gene63658.t1 MTR_4g023200 49.057 318 131 11 1 311 65 358 1.22e-80 249
MS.gene63658.t1 MTR_5g070088 45.075 335 149 8 3 313 63 386 6.83e-79 245
MS.gene63658.t1 MTR_4g023150 47.950 317 136 10 1 311 65 358 2.46e-78 243
MS.gene63658.t1 MTR_8g032390 47.785 316 117 11 2 308 94 370 5.17e-77 240
MS.gene63658.t1 MTR_4g023440 46.006 313 115 7 1 313 74 332 1.27e-76 238
MS.gene63658.t1 MTR_4g118400 47.000 300 113 8 16 307 33 294 5.61e-73 227
MS.gene63658.t1 MTR_4g116320 56.250 224 83 6 91 310 43 255 2.87e-71 221
MS.gene63658.t1 MTR_8g013010 57.407 216 75 5 1 214 109 309 3.38e-70 221
MS.gene63658.t1 MTR_7g079400 41.234 308 158 8 14 312 43 336 6.50e-65 208
MS.gene63658.t1 MTR_4g118370 43.772 281 126 7 32 310 7 257 2.00e-64 204
MS.gene63658.t1 MTR_8g040935 52.830 212 88 4 107 312 2 207 4.44e-64 201
MS.gene63658.t1 MTR_8g433030 43.910 312 102 10 2 308 94 337 1.18e-63 204
MS.gene63658.t1 MTR_4g116330 43.689 309 114 8 10 312 86 340 1.24e-62 202
MS.gene63658.t1 MTR_7g079370 41.104 326 160 9 7 315 48 358 4.36e-62 201
MS.gene63658.t1 MTR_4g121580 43.137 306 124 13 7 303 75 339 8.67e-56 186
MS.gene63658.t1 MTR_6g463430 47.619 210 94 5 109 313 4 202 1.72e-51 169
MS.gene63658.t1 MTR_8g102520 38.924 316 141 11 7 312 48 321 1.77e-51 172
MS.gene63658.t1 MTR_1g055175 47.739 199 94 5 118 312 8 200 4.80e-51 167
MS.gene63658.t1 MTR_4g117530 41.860 258 90 8 63 312 47 252 1.44e-49 166
MS.gene63658.t1 MTR_1g055250 41.991 231 119 8 74 300 34 253 1.57e-49 168
MS.gene63658.t1 MTR_4g117430 51.149 174 62 5 46 217 3 155 2.15e-48 159
MS.gene63658.t1 MTR_4g114700 39.405 269 102 7 42 304 5 218 7.23e-44 150
MS.gene63658.t1 MTR_1g055165 50.000 162 78 3 153 312 157 317 1.57e-42 149
MS.gene63658.t1 MTR_4g116340 50.000 176 72 5 10 180 83 247 2.60e-42 147
MS.gene63658.t1 MTR_4g118360 59.055 127 46 3 190 312 1 125 6.98e-40 136
MS.gene63658.t1 MTR_2g048580 32.362 309 163 11 10 312 73 341 2.13e-39 142
MS.gene63658.t1 MTR_8g098830 42.529 174 86 5 114 282 30 194 2.46e-35 128
MS.gene63658.t1 MTR_7g079420 34.520 281 129 10 45 312 905 1143 3.04e-35 135
MS.gene63658.t1 MTR_7g117720 54.167 144 50 7 7 145 52 184 3.73e-34 126
MS.gene63658.t1 MTR_7g117720 38.926 149 77 6 164 307 132 271 1.58e-14 72.8
MS.gene63658.t1 MTR_5g023000 63.158 95 35 0 218 312 4 98 6.03e-33 117
MS.gene63658.t1 MTR_4g122860 39.901 203 76 7 123 315 156 322 2.92e-31 120
MS.gene63658.t1 MTR_6g034720 42.105 190 87 7 40 223 31 203 1.02e-28 110
MS.gene63658.t1 MTR_7g079450 41.333 150 63 5 174 312 1 136 3.83e-24 95.9
MS.gene63658.t1 MTR_1g068875 28.814 236 140 9 21 243 71 291 6.21e-22 95.1
MS.gene63658.t1 MTR_5g069120 45.736 129 61 2 6 127 51 177 1.24e-21 90.5
MS.gene63658.t1 MTR_4g122840 41.176 136 42 4 181 313 164 264 4.99e-19 85.5
MS.gene63658.t1 MTR_4g133170 30.275 218 123 11 36 238 82 285 5.76e-19 87.0
MS.gene63658.t1 MTR_4g023430 56.098 82 33 1 235 313 141 222 2.46e-18 82.4
MS.gene63658.t1 MTR_2g026010 29.258 229 129 10 35 242 81 297 1.17e-17 83.2
MS.gene63658.t1 MTR_0667s0010 24.742 291 178 10 6 268 57 334 1.20e-17 83.2
MS.gene63658.t1 MTR_8g013005 67.241 58 19 0 255 312 1 58 1.38e-17 75.9
MS.gene63658.t1 MTR_2g024110 25.094 267 166 11 7 259 60 306 4.92e-17 81.3
MS.gene63658.t1 MTR_2g028160 27.237 257 149 11 44 274 107 351 1.22e-16 80.5
MS.gene63658.t1 MTR_2g021155 29.348 184 114 5 59 238 711 882 2.08e-16 80.5
MS.gene63658.t1 MTR_2g021155 28.500 200 125 7 44 238 131 317 3.30e-11 64.7
MS.gene63658.t1 MTR_1g055200 50.000 86 38 2 42 127 5 85 2.18e-16 73.9
MS.gene63658.t1 MTR_2g028130 29.185 233 134 10 47 261 117 336 2.74e-16 79.3
MS.gene63658.t1 MTR_2g025960 26.638 229 129 9 39 242 1 215 1.38e-15 76.3
MS.gene63658.t1 MTR_2g078870 30.000 200 110 8 44 238 58 232 1.87e-15 75.9
MS.gene63658.t1 MTR_2g031640 25.833 240 149 8 18 242 70 295 1.93e-15 76.6
MS.gene63658.t1 MTR_4g070550 25.974 231 134 7 34 238 92 311 5.76e-15 75.5
MS.gene63658.t1 MTR_3g083010 24.535 269 161 9 37 276 102 357 3.00e-14 73.2
MS.gene63658.t1 MTR_2g426070 27.778 234 137 11 57 277 129 343 3.00e-14 73.2
MS.gene63658.t1 MTR_1g068860 25.316 237 150 8 21 243 71 294 8.40e-14 71.6
MS.gene63658.t1 MTR_3g010970 30.151 199 104 8 41 238 152 316 9.80e-14 71.6
MS.gene63658.t1 MTR_2g028140 26.106 226 120 8 44 238 108 317 1.35e-13 71.2
MS.gene63658.t1 MTR_3g083640 27.500 200 114 9 44 238 157 330 3.96e-13 69.7
MS.gene63658.t1 MTR_2g078900 29.670 182 101 8 59 238 153 309 4.95e-13 69.3
MS.gene63658.t1 MTR_4g055060 27.642 246 152 9 8 243 93 322 6.92e-13 68.9
MS.gene63658.t1 MTR_6g053260 28.302 212 125 8 34 238 429 620 7.67e-13 69.3
MS.gene63658.t1 MTR_2g021150 26.357 258 166 9 15 264 87 328 8.02e-13 68.9
MS.gene63658.t1 MTR_6g027910 26.606 218 134 6 38 242 106 310 3.13e-12 67.0
MS.gene63658.t1 MTR_1g094210 27.599 279 146 13 22 284 140 378 3.20e-12 67.0
MS.gene63658.t1 MTR_4g023100 61.404 57 20 1 257 313 65 119 3.41e-12 62.8
MS.gene63658.t1 MTR_1g054725 30.622 209 113 11 43 238 117 306 3.54e-12 67.0
MS.gene63658.t1 MTR_1g097560 25.979 281 175 11 7 281 99 352 3.61e-12 67.0
MS.gene63658.t1 MTR_3g007330 27.354 223 130 9 32 243 127 328 4.10e-12 66.6
MS.gene63658.t1 MTR_8g464770 25.723 311 190 15 17 307 88 377 5.34e-12 66.2
MS.gene63658.t1 MTR_2g031680 25.108 231 144 8 33 248 80 296 5.98e-12 66.2
MS.gene63658.t1 MTR_3g036290 28.986 207 114 9 41 243 117 294 6.88e-12 65.9
MS.gene63658.t1 MTR_2g021190 26.009 223 148 7 20 238 95 304 8.56e-12 65.9
MS.gene63658.t1 MTR_3g010870 25.333 225 137 8 21 238 111 311 1.04e-11 65.5
MS.gene63658.t1 MTR_4g109290 60.377 53 21 0 260 312 135 187 1.19e-11 63.2
MS.gene63658.t1 MTR_7g056390 28.870 239 114 11 41 251 130 340 1.23e-11 65.5
MS.gene63658.t1 MTR_3g010920 25.483 259 145 9 58 304 158 380 1.47e-11 65.1
MS.gene63658.t1 MTR_5g011200 29.952 207 118 11 40 238 114 301 1.56e-11 65.1
MS.gene63658.t1 MTR_2g021252 24.481 241 148 8 3 238 105 316 1.77e-11 64.7
MS.gene63658.t1 MTR_3g007410 27.273 209 128 8 44 243 146 339 2.10e-11 64.7
MS.gene63658.t1 MTR_3g006570 28.218 202 120 8 44 238 118 301 2.19e-11 64.3
MS.gene63658.t1 MTR_4g117550 27.273 275 149 12 53 311 135 374 2.24e-11 64.3
MS.gene63658.t1 MTR_3g030250 28.436 211 107 9 46 242 130 310 2.37e-11 64.3
MS.gene63658.t1 MTR_2g031720 22.936 218 144 5 39 242 92 299 2.42e-11 64.3
MS.gene63658.t1 MTR_2g021050 24.783 230 137 10 28 242 77 285 2.46e-11 64.3
MS.gene63658.t1 MTR_3g007360 27.014 211 126 8 44 243 146 339 2.74e-11 64.3
MS.gene63658.t1 MTR_7g077910 25.974 231 137 7 41 252 42 257 2.76e-11 63.9
MS.gene63658.t1 MTR_4g061340 26.484 219 132 7 38 243 114 316 3.13e-11 63.9
MS.gene63658.t1 MTR_2g024100 28.505 214 117 10 47 238 107 306 3.70e-11 63.9
MS.gene63658.t1 MTR_4g031770 26.667 210 114 9 44 238 101 285 3.75e-11 63.5
MS.gene63658.t1 MTR_1g094210 27.473 273 142 13 22 278 140 372 3.84e-11 63.9
MS.gene63658.t1 MTR_4g013175 26.104 249 139 9 41 259 121 354 4.08e-11 63.9
MS.gene63658.t1 MTR_4g028510 27.208 283 152 11 59 308 139 400 4.10e-11 63.9
MS.gene63658.t1 MTR_3g006670 29.412 204 117 9 44 238 147 332 4.13e-11 63.9
MS.gene63658.t1 MTR_0129s0070 25.979 281 167 12 57 313 136 399 4.59e-11 63.5
MS.gene63658.t1 MTR_8g099460 24.125 257 150 10 12 242 64 301 4.80e-11 63.5
MS.gene63658.t1 MTR_2g025420 23.301 309 179 13 6 278 67 353 5.18e-11 63.5
MS.gene63658.t1 MTR_3g013740 27.805 205 122 7 44 238 150 338 5.44e-11 63.5
MS.gene63658.t1 MTR_2g064960 65.854 41 14 0 272 312 18 58 7.40e-11 57.4
MS.gene63658.t1 MTR_5g037680 24.922 321 179 13 13 304 93 380 8.27e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63658.t1 AT4G12560 29.108 213 136 8 40 239 90 300 2.81e-15 76.3
MS.gene63658.t1 AT4G12560 29.108 213 136 8 40 239 90 300 2.81e-15 76.3
MS.gene63658.t1 AT4G12560 29.108 213 136 8 40 239 90 300 2.81e-15 76.3
MS.gene63658.t1 AT4G22390 26.336 262 166 11 40 282 90 343 1.63e-11 65.1
MS.gene63658.t1 AT4G22390 26.336 262 166 11 40 282 90 343 1.75e-11 65.1

Find 67 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAAATGTGGTCATATTAT+TGG 0.222453 3.2:-23059417 MS.gene63658:CDS
GATTGTGATTTGTGGATATT+TGG 0.226311 3.2:-23059585 MS.gene63658:CDS
TACTCACTTCCCTTATTATA+TGG 0.240722 3.2:-23059769 MS.gene63658:CDS
AAATATTTATGTTGTCTATA+TGG 0.249954 3.2:-23059951 MS.gene63658:CDS
GGGATGAAACAATTGAAATA+TGG 0.254108 3.2:-23059536 MS.gene63658:CDS
TTATGTTGTCTATATGGTTT+TGG 0.265207 3.2:-23059945 MS.gene63658:CDS
TCATTCGCGTCCATATAATA+AGG 0.269202 3.2:+23059759 None:intergenic
ATTCGGGAAGCATAAAATTA+AGG 0.275163 3.2:+23060108 None:intergenic
GTTCTTCAAACATTTATCTA+TGG 0.300796 3.2:-23060031 MS.gene63658:CDS
TTGGAGAATTTCTCAGTGTA+TGG 0.340756 3.2:-23059566 MS.gene63658:CDS
ATTAAAGAGCGTCCCTACTT+TGG 0.356100 3.2:+23059731 None:intergenic
TTCAAGATTAGAATAAGATT+CGG 0.367334 3.2:+23060091 None:intergenic
TATTGTTGGCTTTGATTTAG+TGG 0.370118 3.2:-23059661 MS.gene63658:CDS
TTAAAGAGCGTCCCTACTTT+GGG 0.372779 3.2:+23059732 None:intergenic
TCAAGATTAGAATAAGATTC+GGG 0.401464 3.2:+23060092 None:intergenic
ACTGCTGTCCTATCTATTGA+TGG 0.424183 3.2:-23059474 MS.gene63658:CDS
AACCTATAGATTGTGATTTG+TGG 0.425796 3.2:-23059593 MS.gene63658:CDS
GGATGAAACAATTGAAATAT+GGG 0.428889 3.2:-23059535 MS.gene63658:CDS
AGGTATTTGTTTGTGATGTC+CGG 0.430912 3.2:+23059998 None:intergenic
ATTGGAACAAATGGTGGTAC+TGG 0.446274 3.2:-23059399 MS.gene63658:CDS
AATCACAATCTATAGGTTCA+TGG 0.456461 3.2:+23059598 None:intergenic
GGTTCATGGTCAAAACCATC+TGG 0.456773 3.2:+23059612 None:intergenic
TCTTTAATGAGACTATTCAT+TGG 0.456901 3.2:-23059716 MS.gene63658:CDS
ATCCACAAATCACAATCTAT+AGG 0.460799 3.2:+23059591 None:intergenic
TGCAAATGCCATCAATAGAT+AGG 0.461230 3.2:+23059466 None:intergenic
CCATTCAAGAGATGATTACT+TGG 0.463155 3.2:-23059916 MS.gene63658:CDS
TTTCTCAGTGTATGGGCTAT+GGG 0.463220 3.2:-23059558 MS.gene63658:CDS
TCTAAATTGGAAGGAGGAAA+AGG 0.467688 3.2:+23059978 None:intergenic
ATTTCTCAGTGTATGGGCTA+TGG 0.470971 3.2:-23059559 MS.gene63658:CDS
TTATCAATGGATGTTATTGT+TGG 0.479970 3.2:-23059675 MS.gene63658:CDS
TGGGTCTATACTCGTATCAT+AGG 0.480780 3.2:+23059881 None:intergenic
CATTCGCGTCCATATAATAA+GGG 0.483397 3.2:+23059760 None:intergenic
TAACATCCATTGATAAATCA+TGG 0.489522 3.2:+23059682 None:intergenic
CTCTGTATACATAGCCACTA+GGG 0.491428 3.2:+23059308 None:intergenic
GTGAGTATACTTCACCAACC+TGG 0.496759 3.2:+23059785 None:intergenic
TCTCGTTGAGAGCTAATACA+TGG 0.498785 3.2:-23059824 MS.gene63658:CDS
GGTCATATTATTGGAACAAA+TGG 0.505789 3.2:-23059408 MS.gene63658:CDS
CCAAGTAATCATCTCTTGAA+TGG 0.510533 3.2:+23059916 None:intergenic
GGAACGAAGTTGAGTGTCCC+AGG 0.516125 3.2:-23059803 MS.gene63658:CDS
TGGAGAATTTCTCAGTGTAT+GGG 0.516571 3.2:-23059565 MS.gene63658:CDS
AGTACAAAGTGCATTCGTCT+TGG 0.519123 3.2:-23059503 MS.gene63658:CDS
TCAATTTGCTCATTGTCACC+AGG 0.530329 3.2:+23059270 None:intergenic
GAAATTAAAAGTTCATGTAG+AGG 0.538733 3.2:-23060071 MS.gene63658:CDS
GCGAATGATGATCCCAAAGT+AGG 0.546958 3.2:-23059744 MS.gene63658:CDS
ATGCTTCAAAATCTATAGAT+CGG 0.549230 3.2:+23060165 None:intergenic
ATAGATCGGATTTCAAGAGC+TGG 0.553736 3.2:+23060179 None:intergenic
TTCAAGAGATGATTACTTGG+TGG 0.560339 3.2:-23059913 MS.gene63658:CDS
TTCTCAGTGTATGGGCTATG+GGG 0.565300 3.2:-23059557 MS.gene63658:CDS
TTGGCTTTGATTTAGTGGAA+AGG 0.565737 3.2:-23059656 MS.gene63658:CDS
CAATTTGCTCATTGTCACCA+GGG 0.568496 3.2:+23059271 None:intergenic
CGAATGATGATCCCAAAGTA+GGG 0.573102 3.2:-23059743 MS.gene63658:CDS
CCAATATGCTCTACAAAATG+TGG 0.574609 3.2:-23059429 MS.gene63658:CDS
TGTTTGTGATGTCCGGTCGA+TGG 0.582419 3.2:+23060005 None:intergenic
TGAGTATACTTCACCAACCT+GGG 0.584734 3.2:+23059786 None:intergenic
GAGCAAATTGAAGAAGAATG+AGG 0.587599 3.2:-23059258 None:intergenic
TATCTATGGAATCCATCGAC+CGG 0.592196 3.2:-23060017 MS.gene63658:CDS
CATATTATTGGAACAAATGG+TGG 0.593852 3.2:-23059405 MS.gene63658:CDS
TTTGCATCTAAATTGGAAGG+AGG 0.595652 3.2:+23059972 None:intergenic
CGAAGTTGAGTGTCCCAGGT+TGG 0.597435 3.2:-23059799 MS.gene63658:CDS
TCTCAGTGTATGGGCTATGG+GGG 0.608949 3.2:-23059556 MS.gene63658:CDS
TTGGTGAAGTACGATGGAAA+TGG 0.611532 3.2:-23059375 MS.gene63658:CDS
AACAAATGGTGGTACTGGAT+TGG 0.613049 3.2:-23059394 MS.gene63658:CDS
ACTCTGTATACATAGCCACT+AGG 0.618200 3.2:+23059307 None:intergenic
CGAACCATGTGCACCCCTAG+TGG 0.626155 3.2:-23059322 MS.gene63658:CDS
ATAGCCACTAGGGGTGCACA+TGG 0.643218 3.2:+23059318 None:intergenic
ACTGGATTGGTGAAGTACGA+TGG 0.682707 3.2:-23059381 MS.gene63658:CDS
TCTGTATACATAGCCACTAG+GGG 0.698983 3.2:+23059309 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGAAATATTATCTTTTATT+GGG + chr3.2:23059589-23059608 None:intergenic 10.0%
!! AAATATTTATGTTGTCTATA+TGG - chr3.2:23059497-23059516 MS.gene63658:CDS 15.0%
!!! GATGAAATATTATCTTTTAT+TGG + chr3.2:23059590-23059609 None:intergenic 15.0%
!!! TAAATATTTTGCATCTAAAT+TGG + chr3.2:23059486-23059505 None:intergenic 15.0%
!! TTCAAGATTAGAATAAGATT+CGG + chr3.2:23059360-23059379 None:intergenic 20.0%
! ACAAAATGTGGTCATATTAT+TGG - chr3.2:23060031-23060050 MS.gene63658:CDS 25.0%
! ATGCTTCAAAATCTATAGAT+CGG + chr3.2:23059286-23059305 None:intergenic 25.0%
! GAAATTAAAAGTTCATGTAG+AGG - chr3.2:23059377-23059396 MS.gene63658:CDS 25.0%
! GGATGAAACAATTGAAATAT+GGG - chr3.2:23059913-23059932 MS.gene63658:CDS 25.0%
! GTTCTTCAAACATTTATCTA+TGG - chr3.2:23059417-23059436 MS.gene63658:CDS 25.0%
! TAACATCCATTGATAAATCA+TGG + chr3.2:23059769-23059788 None:intergenic 25.0%
! TCAAGATTAGAATAAGATTC+GGG + chr3.2:23059359-23059378 None:intergenic 25.0%
! TCTTTAATGAGACTATTCAT+TGG - chr3.2:23059732-23059751 MS.gene63658:CDS 25.0%
! TTATCAATGGATGTTATTGT+TGG - chr3.2:23059773-23059792 MS.gene63658:CDS 25.0%
!! TATGTTGTCTATATGGTTTT+GGG - chr3.2:23059504-23059523 MS.gene63658:CDS 25.0%
!! TATTTTGCATCTAAATTGGA+AGG + chr3.2:23059482-23059501 None:intergenic 25.0%
!! TTATGTTGTCTATATGGTTT+TGG - chr3.2:23059503-23059522 MS.gene63658:CDS 25.0%
AACCTATAGATTGTGATTTG+TGG - chr3.2:23059855-23059874 MS.gene63658:CDS 30.0%
AATCACAATCTATAGGTTCA+TGG + chr3.2:23059853-23059872 None:intergenic 30.0%
ATCCACAAATCACAATCTAT+AGG + chr3.2:23059860-23059879 None:intergenic 30.0%
CATATTATTGGAACAAATGG+TGG - chr3.2:23060043-23060062 MS.gene63658:CDS 30.0%
GGGATGAAACAATTGAAATA+TGG - chr3.2:23059912-23059931 MS.gene63658:CDS 30.0%
GGTCATATTATTGGAACAAA+TGG - chr3.2:23060040-23060059 MS.gene63658:CDS 30.0%
TACTCACTTCCCTTATTATA+TGG - chr3.2:23059679-23059698 MS.gene63658:CDS 30.0%
! ACATTTTGTAGAGCATATTG+GGG + chr3.2:23060020-23060039 None:intergenic 30.0%
! ATTCGGGAAGCATAAAATTA+AGG + chr3.2:23059343-23059362 None:intergenic 30.0%
! CACATTTTGTAGAGCATATT+GGG + chr3.2:23060021-23060040 None:intergenic 30.0%
! GATTGTGATTTGTGGATATT+TGG - chr3.2:23059863-23059882 MS.gene63658:CDS 30.0%
! TTTTCGCCATGATTTATCAA+TGG - chr3.2:23059760-23059779 MS.gene63658:CDS 30.0%
!! TATTGTTGGCTTTGATTTAG+TGG - chr3.2:23059787-23059806 MS.gene63658:CDS 30.0%
AGGTATTTGTTTGTGATGTC+CGG + chr3.2:23059453-23059472 None:intergenic 35.0%
CATTCGCGTCCATATAATAA+GGG + chr3.2:23059691-23059710 None:intergenic 35.0%
CCAAGTAATCATCTCTTGAA+TGG + chr3.2:23059535-23059554 None:intergenic 35.0%
CCAATATGCTCTACAAAATG+TGG - chr3.2:23060019-23060038 MS.gene63658:CDS 35.0%
CCATTCAAGAGATGATTACT+TGG - chr3.2:23059532-23059551 MS.gene63658:CDS 35.0%
TCATTCGCGTCCATATAATA+AGG + chr3.2:23059692-23059711 None:intergenic 35.0%
TCTAAATTGGAAGGAGGAAA+AGG + chr3.2:23059473-23059492 None:intergenic 35.0%
TGCAAATGCCATCAATAGAT+AGG + chr3.2:23059985-23060004 None:intergenic 35.0%
TGGAGAATTTCTCAGTGTAT+GGG - chr3.2:23059883-23059902 MS.gene63658:CDS 35.0%
TTCAAGAGATGATTACTTGG+TGG - chr3.2:23059535-23059554 MS.gene63658:CDS 35.0%
TTGGAGAATTTCTCAGTGTA+TGG - chr3.2:23059882-23059901 MS.gene63658:CDS 35.0%
TTTGCATCTAAATTGGAAGG+AGG + chr3.2:23059479-23059498 None:intergenic 35.0%
! CATTTTGTAGAGCATATTGG+GGG + chr3.2:23060019-23060038 None:intergenic 35.0%
! CCACATTTTGTAGAGCATAT+TGG + chr3.2:23060022-23060041 None:intergenic 35.0%
! TTAGAGATGCATTTTCCAGA+TGG - chr3.2:23059821-23059840 MS.gene63658:CDS 35.0%
!! TTGGCTTTGATTTAGTGGAA+AGG - chr3.2:23059792-23059811 MS.gene63658:CDS 35.0%
ACTCTGTATACATAGCCACT+AGG + chr3.2:23060144-23060163 None:intergenic 40.0%
ACTGCTGTCCTATCTATTGA+TGG - chr3.2:23059974-23059993 MS.gene63658:CDS 40.0%
AGTACAAAGTGCATTCGTCT+TGG - chr3.2:23059945-23059964 MS.gene63658:CDS 40.0%
ATAGATCGGATTTCAAGAGC+TGG + chr3.2:23059272-23059291 None:intergenic 40.0%
ATTGGAACAAATGGTGGTAC+TGG - chr3.2:23060049-23060068 MS.gene63658:CDS 40.0%
CGAATGATGATCCCAAAGTA+GGG - chr3.2:23059705-23059724 MS.gene63658:CDS 40.0%
CTCTGTATACATAGCCACTA+GGG + chr3.2:23060143-23060162 None:intergenic 40.0%
TATCTATGGAATCCATCGAC+CGG - chr3.2:23059431-23059450 MS.gene63658:CDS 40.0%
TCTCGTTGAGAGCTAATACA+TGG - chr3.2:23059624-23059643 MS.gene63658:CDS 40.0%
TCTGTATACATAGCCACTAG+GGG + chr3.2:23060142-23060161 None:intergenic 40.0%
TGAGTATACTTCACCAACCT+GGG + chr3.2:23059665-23059684 None:intergenic 40.0%
TGGGTCTATACTCGTATCAT+AGG + chr3.2:23059570-23059589 None:intergenic 40.0%
TTGGTGAAGTACGATGGAAA+TGG - chr3.2:23060073-23060092 MS.gene63658:CDS 40.0%
! AACAAATGGTGGTACTGGAT+TGG - chr3.2:23060054-23060073 MS.gene63658:CDS 40.0%
! ATTTCTCAGTGTATGGGCTA+TGG - chr3.2:23059889-23059908 MS.gene63658:CDS 40.0%
! CAATTTGCTCATTGTCACCA+GGG + chr3.2:23060180-23060199 None:intergenic 40.0%
! TCAATTTGCTCATTGTCACC+AGG + chr3.2:23060181-23060200 None:intergenic 40.0%
! TTTCTCAGTGTATGGGCTAT+GGG - chr3.2:23059890-23059909 MS.gene63658:CDS 40.0%
!! ATTAAAGAGCGTCCCTACTT+TGG + chr3.2:23059720-23059739 None:intergenic 40.0%
!! TTAAAGAGCGTCCCTACTTT+GGG + chr3.2:23059719-23059738 None:intergenic 40.0%
GCGAATGATGATCCCAAAGT+AGG - chr3.2:23059704-23059723 MS.gene63658:CDS 45.0%
GGTTCATGGTCAAAACCATC+TGG + chr3.2:23059839-23059858 None:intergenic 45.0%
GTGAGTATACTTCACCAACC+TGG + chr3.2:23059666-23059685 None:intergenic 45.0%
! TTCTCAGTGTATGGGCTATG+GGG - chr3.2:23059891-23059910 MS.gene63658:CDS 45.0%
!! ACTGGATTGGTGAAGTACGA+TGG - chr3.2:23060067-23060086 MS.gene63658:CDS 45.0%
TGTTTGTGATGTCCGGTCGA+TGG + chr3.2:23059446-23059465 None:intergenic 50.0%
! TCTCAGTGTATGGGCTATGG+GGG - chr3.2:23059892-23059911 MS.gene63658:CDS 50.0%
!! GAGTCTCTGCTTTTACTCCC+TGG - chr3.2:23060160-23060179 MS.gene63658:CDS 50.0%
CGAAGTTGAGTGTCCCAGGT+TGG - chr3.2:23059649-23059668 MS.gene63658:CDS 55.0%
GGAACGAAGTTGAGTGTCCC+AGG - chr3.2:23059645-23059664 MS.gene63658:CDS 55.0%
! ATAGCCACTAGGGGTGCACA+TGG + chr3.2:23060133-23060152 None:intergenic 55.0%
CGAACCATGTGCACCCCTAG+TGG - chr3.2:23060126-23060145 MS.gene63658:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 23059260 23060210 23059260 ID=MS.gene63658
chr3.2 mRNA 23059260 23060210 23059260 ID=MS.gene63658.t1;Parent=MS.gene63658
chr3.2 exon 23059260 23060210 23059260 ID=MS.gene63658.t1.exon1;Parent=MS.gene63658.t1
chr3.2 CDS 23059260 23060210 23059260 ID=cds.MS.gene63658.t1;Parent=MS.gene63658.t1
Gene Sequence

>MS.gene63658

ATGTCAACTCCAGCTCTTGAAATCCGATCTATAGATTTTGAAGCATCGCTTAACGATGATTCTGCTTCTATTTCACTGAACCTTAATTTTATGCTTCCCGAATCTTATTCTAATCTTGAAATTAAAAGTTCATGTAGAGGATTTATAGTTTTGACTTGTTCTTCAAACATTTATCTATGGAATCCATCGACCGGACATCACAAACAAATACCTTTTCCTCCTTCCAATTTAGATGCAAAATATTTATGTTGTCTATATGGTTTTGGGTATGACCATTCAAGAGATGATTACTTGGTGGTATCAGTGTCCTATGATACGAGTATAGACCCAATAAAAGATAATATTTCATCACATTTGAAGTTTTTCTCGTTGAGAGCTAATACATGGAACGAAGTTGAGTGTCCCAGGTTGGTGAAGTATACTCACTTCCCTTATTATATGGACGCGAATGATGATCCCAAAGTAGGGACGCTCTTTAATGAGACTATTCATTGGTTCTCTTTTCGCCATGATTTATCAATGGATGTTATTGTTGGCTTTGATTTAGTGGAAAGGAAACTTTTAGAGATGCATTTTCCAGATGGTTTTGACCATGAACCTATAGATTGTGATTTGTGGATATTTGGAGAATTTCTCAGTGTATGGGCTATGGGGGATGAAACAATTGAAATATGGGTTATGAAAGAGTACAAAGTGCATTCGTCTTGGACTAAGACTGCTGTCCTATCTATTGATGGCATTTGCATTCAGTATGATCCCCCAATATGCTCTACAAAATGTGGTCATATTATTGGAACAAATGGTGGTACTGGATTGGTGAAGTACGATGGAAATGGTCAGTTGCTAGAGAATCGCTCATACTATAACGAACCATGTGCACCCCTAGTGGCTATGTATACAGAGTCTCTGCTTTTACTCCCTGGTGACAATGAGCAAATTGAAGAAGAATGA
Protein sequence

>MS.gene63658.t1

MSTPALEIRSIDFEASLNDDSASISLNLNFMLPESYSNLEIKSSCRGFIVLTCSSNIYLWNPSTGHHKQIPFPPSNLDAKYLCCLYGFGYDHSRDDYLVVSVSYDTSIDPIKDNISSHLKFFSLRANTWNEVECPRLVKYTHFPYYMDANDDPKVGTLFNETIHWFSFRHDLSMDVIVGFDLVERKLLEMHFPDGFDHEPIDCDLWIFGEFLSVWAMGDETIEIWVMKEYKVHSSWTKTAVLSIDGICIQYDPPICSTKCGHIIGTNGGTGLVKYDGNGQLLENRSYYNEPCAPLVAMYTESLLLLPGDNEQIEEE