AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51208

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51208.t1 MTR_8g102520 65.753 219 71 3 1 217 106 322 7.41e-94 277
MS.gene51208.t1 MTR_7g079400 57.848 223 86 4 1 217 117 337 2.63e-80 243
MS.gene51208.t1 MTR_8g098830 64.205 176 59 2 18 191 27 200 1.44e-75 228
MS.gene51208.t1 MTR_7g079450 74.820 139 32 2 79 217 2 137 2.37e-70 211
MS.gene51208.t1 MTR_7g079370 55.760 217 92 4 2 215 139 354 7.12e-70 217
MS.gene51208.t1 MTR_2g048580 53.000 200 79 5 22 217 154 342 1.05e-56 183
MS.gene51208.t1 MTR_6g060650 47.926 217 103 7 2 216 164 372 1.29e-55 181
MS.gene51208.t1 MTR_7g079420 54.645 183 72 4 42 217 966 1144 1.80e-52 181
MS.gene51208.t1 MTR_4g117720 46.119 219 104 7 2 213 162 373 1.29e-50 168
MS.gene51208.t1 MTR_5g070080 44.796 221 109 7 2 217 174 386 2.26e-46 157
MS.gene51208.t1 MTR_4g109270 43.379 219 108 6 3 217 158 364 1.08e-45 155
MS.gene51208.t1 MTR_4g094365 44.344 221 107 8 2 217 155 364 1.17e-45 155
MS.gene51208.t1 MTR_4g118380 47.236 199 87 8 2 189 166 357 1.56e-45 155
MS.gene51208.t1 MTR_8g433030 43.925 214 108 6 4 213 133 338 3.13e-45 153
MS.gene51208.t1 MTR_4g116330 45.662 219 105 7 4 217 171 380 3.28e-45 154
MS.gene51208.t1 MTR_4g109280 44.615 195 100 5 26 217 170 359 4.82e-45 153
MS.gene51208.t1 MTR_8g032390 43.925 214 108 6 4 213 166 371 7.44e-45 153
MS.gene51208.t1 MTR_1g055175 40.909 198 114 3 22 217 5 201 3.38e-44 147
MS.gene51208.t1 MTR_4g116320 43.396 212 112 5 3 211 46 252 3.68e-44 148
MS.gene51208.t1 MTR_3g103020 43.439 221 112 7 3 217 165 378 4.62e-44 151
MS.gene51208.t1 MTR_8g040935 43.719 199 101 7 26 217 14 208 9.12e-43 143
MS.gene51208.t1 MTR_4g118400 46.875 192 74 9 26 211 125 294 2.21e-42 145
MS.gene51208.t1 MTR_3g034180 40.693 231 109 8 2 216 270 488 1.17e-41 147
MS.gene51208.t1 MTR_4g117850 41.364 220 114 8 3 213 163 376 5.90e-41 143
MS.gene51208.t1 MTR_4g122850 41.364 220 120 8 2 217 150 364 1.12e-40 142
MS.gene51208.t1 MTR_4g023130 42.254 213 102 7 2 210 111 306 2.59e-40 140
MS.gene51208.t1 MTR_4g023080 42.254 213 102 7 2 210 152 347 6.87e-40 140
MS.gene51208.t1 MTR_5g070088 43.439 221 107 8 2 213 170 381 1.54e-39 140
MS.gene51208.t1 MTR_5g070130 42.081 221 115 8 2 213 156 372 2.47e-39 139
MS.gene51208.t1 MTR_8g433040 40.991 222 105 8 2 217 163 364 7.91e-39 137
MS.gene51208.t1 MTR_8g032380 40.991 222 105 8 2 217 163 364 7.91e-39 137
MS.gene51208.t1 MTR_3g034130 39.901 203 100 7 2 190 156 350 1.14e-38 137
MS.gene51208.t1 MTR_1g055195 38.767 227 111 8 2 217 269 478 1.45e-38 139
MS.gene51208.t1 MTR_4g117750 39.640 222 125 4 2 216 139 358 2.74e-37 135
MS.gene51208.t1 MTR_4g117750 41.912 136 61 6 90 211 346 477 1.46e-18 83.6
MS.gene51208.t1 MTR_6g463430 43.077 195 100 7 26 217 16 202 3.01e-37 129
MS.gene51208.t1 MTR_1g055135 39.286 224 124 5 2 217 156 375 3.32e-37 133
MS.gene51208.t1 MTR_4g117530 37.327 217 89 7 3 217 82 253 1.00e-36 129
MS.gene51208.t1 MTR_4g118370 39.070 215 108 5 2 214 64 257 2.04e-36 128
MS.gene51208.t1 MTR_5g097210 38.967 213 124 6 2 212 150 358 3.29e-36 130
MS.gene51208.t1 MTR_4g117770 42.342 222 110 8 3 217 157 367 3.48e-35 128
MS.gene51208.t1 MTR_4g117810 42.342 222 110 8 3 217 157 367 3.48e-35 128
MS.gene51208.t1 MTR_4g021785 39.091 220 114 8 2 217 152 355 4.79e-35 127
MS.gene51208.t1 MTR_1g055165 42.331 163 91 3 57 217 157 318 1.79e-34 125
MS.gene51208.t1 MTR_4g117780 40.991 222 113 8 3 217 157 367 1.03e-33 124
MS.gene51208.t1 MTR_4g023090 37.555 229 106 8 4 211 166 378 1.73e-33 124
MS.gene51208.t1 MTR_3g034160 37.118 229 116 8 4 216 158 374 3.90e-33 122
MS.gene51208.t1 MTR_4g023190 39.111 225 105 8 2 215 154 357 5.71e-32 119
MS.gene51208.t1 MTR_4g023070 37.768 233 118 9 2 216 146 369 1.57e-31 118
MS.gene51208.t1 MTR_4g023120 37.607 234 119 9 2 217 146 370 2.11e-31 118
MS.gene51208.t1 MTR_1g055250 37.559 213 124 7 2 210 56 263 3.43e-31 116
MS.gene51208.t1 MTR_4g117870 37.615 218 92 8 2 217 92 267 2.83e-30 112
MS.gene51208.t1 MTR_4g116330 38.813 219 81 8 4 217 171 341 1.09e-28 110
MS.gene51208.t1 MTR_4g023380 37.264 212 98 7 26 211 169 371 3.13e-28 109
MS.gene51208.t1 MTR_4g023150 38.579 197 106 7 26 215 170 358 5.62e-28 108
MS.gene51208.t1 MTR_4g023200 36.283 226 111 8 2 215 154 358 1.66e-27 107
MS.gene51208.t1 MTR_4g118360 47.934 121 59 3 94 211 1 120 2.47e-27 101
MS.gene51208.t1 MTR_5g023000 53.684 95 38 4 126 217 8 99 6.22e-24 91.7
MS.gene51208.t1 MTR_4g023440 33.486 218 95 7 2 217 163 332 7.48e-23 94.7
MS.gene51208.t1 MTR_4g122860 35.385 195 87 8 31 217 157 320 4.62e-22 92.4
MS.gene51208.t1 MTR_7g117720 51.042 96 43 2 121 212 177 272 1.33e-19 84.7
MS.gene51208.t1 MTR_4g117430 38.793 116 58 3 2 117 49 151 2.89e-18 78.6
MS.gene51208.t1 MTR_4g121580 36.150 213 92 14 2 207 164 339 1.26e-16 77.8
MS.gene51208.t1 MTR_8g013010 41.026 117 59 4 2 118 203 309 4.97e-16 75.5
MS.gene51208.t1 MTR_4g114700 33.163 196 60 8 26 208 81 218 3.82e-14 69.3
MS.gene51208.t1 MTR_2g028130 29.412 153 88 7 26 166 195 339 1.62e-12 66.2
MS.gene51208.t1 MTR_2g024110 25.287 174 108 6 2 161 147 312 7.59e-12 63.9
MS.gene51208.t1 MTR_1g068875 28.409 176 101 6 5 166 150 314 3.91e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATTGCCTTTGATTTAA+AGG 0.116438 7.3:+27819616 MS.gene51208:CDS
CGATGATTACATGGTAGTTT+TGG 0.187638 7.3:+27819385 MS.gene51208:CDS
CTGCTTCTGATTTGCCTTAT+AGG 0.209679 7.3:+27819498 MS.gene51208:CDS
GGCAATAATAACATTGCTTT+TGG 0.240132 7.3:-27819603 None:intergenic
TCTCCTGTAATTGTAGTTGA+TGG 0.260726 7.3:-27819411 None:intergenic
GTTTATGATTTGATGGTATT+TGG 0.262482 7.3:+27819692 MS.gene51208:CDS
CACAACTTGTAAAGCATATT+GGG 0.268178 7.3:-27819841 None:intergenic
CCACAACTTGTAAAGCATAT+TGG 0.287389 7.3:-27819842 None:intergenic
GAGTTTGCAGTCTTCCTATA+AGG 0.303546 7.3:-27819512 None:intergenic
ATTGGTCTAAAATTAAGATA+TGG 0.313518 7.3:+27819741 MS.gene51208:CDS
ACTCTATATACACCGTTGTT+TGG 0.341638 7.3:-27819970 None:intergenic
CTCTATATACACCGTTGTTT+GGG 0.346270 7.3:-27819969 None:intergenic
AATTTATGTTTATGATTTGA+TGG 0.366734 7.3:+27819685 MS.gene51208:CDS
TCTTGAATGATTCTATTCAT+TGG 0.377956 7.3:+27819561 MS.gene51208:CDS
GAATGATTCTATTCATTGGC+TGG 0.387700 7.3:+27819565 MS.gene51208:CDS
TAAGCTTGCTCAGCGCCATC+AGG 0.389919 7.3:-27820007 None:intergenic
GAGCCATCAACTACAATTAC+AGG 0.392671 7.3:+27819408 MS.gene51208:CDS
AAGACTGCAAACTCTGAATA+TGG 0.417528 7.3:+27819521 MS.gene51208:CDS
AATACAAAGTTCATTCATCT+TGG 0.418592 7.3:+27819774 MS.gene51208:CDS
ACAAGTTGTGGTGATATAGT+TGG 0.420850 7.3:+27819854 MS.gene51208:CDS
CTGAATATGGTCCCACTGTC+GGG 0.423661 7.3:+27819534 MS.gene51208:CDS
CCAATATGCTTTACAAGTTG+TGG 0.444016 7.3:+27819842 MS.gene51208:CDS
TTGGCGGACTTATCAGTGTA+TGG 0.447839 7.3:+27819711 MS.gene51208:CDS
ATTCCACGAGAAAAGTCATC+TGG 0.461053 7.3:-27819665 None:intergenic
TCTCATTGAGAGCTAATAAG+TGG 0.462649 7.3:+27819462 MS.gene51208:CDS
GGACCAGATGATGCTGCTGC+TGG 0.464910 7.3:+27819875 MS.gene51208:CDS
AATCCAGCAGCAGCATCATC+TGG 0.480262 7.3:-27819878 None:intergenic
CATGTCAAGCGATGATTACA+TGG 0.486681 7.3:+27819376 None:intergenic
TTGGTCTAAAATTAAGATAT+GGG 0.501065 7.3:+27819742 MS.gene51208:CDS
CTGCTAGAGTATCACTCCTA+TGG 0.518974 7.3:+27819926 MS.gene51208:CDS
TATGATTTGATGGTATTTGG+CGG 0.556414 7.3:+27819695 MS.gene51208:CDS
TTTATAGTAAGAGGCGCCAT+AGG 0.557815 7.3:-27819942 None:intergenic
TCTGAATATGGTCCCACTGT+CGG 0.575242 7.3:+27819533 MS.gene51208:CDS
TCAGTGTATGGAATATCGAT+TGG 0.585066 7.3:+27819723 MS.gene51208:CDS
TCTCTACTTTCACTCCCTGA+TGG 0.614093 7.3:+27819992 MS.gene51208:CDS
TTCAAGAATGACCCGACAGT+GGG 0.615485 7.3:-27819545 None:intergenic
AAGCTTGCTCAGCGCCATCA+GGG 0.627984 7.3:-27820006 None:intergenic
ACAACTTGTAAAGCATATTG+GGG 0.651604 7.3:-27819840 None:intergenic
TAATAAGTGGAAACAAGTTG+AGG 0.653574 7.3:+27819475 MS.gene51208:CDS
TTAGCGAAGCTTAATGACAA+AGG 0.665656 7.3:+27819899 MS.gene51208:CDS
ATTCAAGAATGACCCGACAG+TGG 0.672977 7.3:-27819546 None:intergenic
CTATAAAAGATCCCAAACAA+CGG 0.697525 7.3:+27819958 MS.gene51208:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTATGTTTATGATTTGA+TGG + chr7.3:27819685-27819704 MS.gene51208:CDS 15.0%
!!! ATTGGTCTAAAATTAAGATA+TGG + chr7.3:27819741-27819760 MS.gene51208:CDS 20.0%
!!! TATTATTGCCTTTGATTTAA+AGG + chr7.3:27819616-27819635 MS.gene51208:CDS 20.0%
!!! TGTATTTTCCTTTAAATCAA+AGG - chr7.3:27819627-27819646 None:intergenic 20.0%
!!! TTGGTCTAAAATTAAGATAT+GGG + chr7.3:27819742-27819761 MS.gene51208:CDS 20.0%
! AATACAAAGTTCATTCATCT+TGG + chr7.3:27819774-27819793 MS.gene51208:CDS 25.0%
! TCTTGAATGATTCTATTCAT+TGG + chr7.3:27819561-27819580 MS.gene51208:CDS 25.0%
!! GTTTATGATTTGATGGTATT+TGG + chr7.3:27819692-27819711 MS.gene51208:CDS 25.0%
ACAACTTGTAAAGCATATTG+GGG - chr7.3:27819843-27819862 None:intergenic 30.0%
CACAACTTGTAAAGCATATT+GGG - chr7.3:27819844-27819863 None:intergenic 30.0%
CTATAAAAGATCCCAAACAA+CGG + chr7.3:27819958-27819977 MS.gene51208:CDS 30.0%
TAATAAGTGGAAACAAGTTG+AGG + chr7.3:27819475-27819494 MS.gene51208:CDS 30.0%
! CTTGAATTTTCTTTTTCTCC+CGG + chr7.3:27819806-27819825 MS.gene51208:CDS 30.0%
! GATGATTACATGGTAGTTTT+GGG + chr7.3:27819386-27819405 MS.gene51208:CDS 30.0%
! GGCAATAATAACATTGCTTT+TGG - chr7.3:27819606-27819625 None:intergenic 30.0%
! TATGATTTGATGGTATTTGG+CGG + chr7.3:27819695-27819714 MS.gene51208:CDS 30.0%
! TTGGGATCTTTTATAGTAAG+AGG - chr7.3:27819954-27819973 None:intergenic 30.0%
AAAAACTAGCTCGATTGACT+TGG + chr7.3:27819433-27819452 MS.gene51208:CDS 35.0%
AAGACTGCAAACTCTGAATA+TGG + chr7.3:27819521-27819540 MS.gene51208:CDS 35.0%
ACAAGTTGTGGTGATATAGT+TGG + chr7.3:27819854-27819873 MS.gene51208:CDS 35.0%
ACTCTATATACACCGTTGTT+TGG - chr7.3:27819973-27819992 None:intergenic 35.0%
CCAATATGCTTTACAAGTTG+TGG + chr7.3:27819842-27819861 MS.gene51208:CDS 35.0%
CCACAACTTGTAAAGCATAT+TGG - chr7.3:27819845-27819864 None:intergenic 35.0%
CTCTATATACACCGTTGTTT+GGG - chr7.3:27819972-27819991 None:intergenic 35.0%
GAATGATTCTATTCATTGGC+TGG + chr7.3:27819565-27819584 MS.gene51208:CDS 35.0%
TCAGTGTATGGAATATCGAT+TGG + chr7.3:27819723-27819742 MS.gene51208:CDS 35.0%
TCTCATTGAGAGCTAATAAG+TGG + chr7.3:27819462-27819481 MS.gene51208:CDS 35.0%
TCTCCTGTAATTGTAGTTGA+TGG - chr7.3:27819414-27819433 None:intergenic 35.0%
TTAGCGAAGCTTAATGACAA+AGG + chr7.3:27819899-27819918 MS.gene51208:CDS 35.0%
! CGATGATTACATGGTAGTTT+TGG + chr7.3:27819385-27819404 MS.gene51208:CDS 35.0%
ATTCCACGAGAAAAGTCATC+TGG - chr7.3:27819668-27819687 None:intergenic 40.0%
GAGCCATCAACTACAATTAC+AGG + chr7.3:27819408-27819427 MS.gene51208:CDS 40.0%
GAGTTTGCAGTCTTCCTATA+AGG - chr7.3:27819515-27819534 None:intergenic 40.0%
! ATTTTCTTTTTCTCCCGGTC+TGG + chr7.3:27819811-27819830 MS.gene51208:CDS 40.0%
! CTGCTTCTGATTTGCCTTAT+AGG + chr7.3:27819498-27819517 MS.gene51208:CDS 40.0%
! TTTTCTTTTTCTCCCGGTCT+GGG + chr7.3:27819812-27819831 MS.gene51208:CDS 40.0%
!! TTTATAGTAAGAGGCGCCAT+AGG - chr7.3:27819945-27819964 None:intergenic 40.0%
ATTCAAGAATGACCCGACAG+TGG - chr7.3:27819549-27819568 None:intergenic 45.0%
TATTGGGGAAAAACCCAGAC+CGG - chr7.3:27819828-27819847 None:intergenic 45.0%
TCTCTACTTTCACTCCCTGA+TGG + chr7.3:27819992-27820011 MS.gene51208:CDS 45.0%
TCTGAATATGGTCCCACTGT+CGG + chr7.3:27819533-27819552 MS.gene51208:CDS 45.0%
TTCAAGAATGACCCGACAGT+GGG - chr7.3:27819548-27819567 None:intergenic 45.0%
! CTGCTAGAGTATCACTCCTA+TGG + chr7.3:27819926-27819945 MS.gene51208:CDS 45.0%
! CTTCCAGATGACTTTTCTCG+TGG + chr7.3:27819662-27819681 MS.gene51208:CDS 45.0%
!! TTGGCGGACTTATCAGTGTA+TGG + chr7.3:27819711-27819730 MS.gene51208:CDS 45.0%
AATCCAGCAGCAGCATCATC+TGG - chr7.3:27819881-27819900 None:intergenic 50.0%
ATTGGGGAAAAACCCAGACC+GGG - chr7.3:27819827-27819846 None:intergenic 50.0%
CTGAATATGGTCCCACTGTC+GGG + chr7.3:27819534-27819553 MS.gene51208:CDS 50.0%
! AAGCTTGCTCAGCGCCATCA+GGG - chr7.3:27820009-27820028 None:intergenic 55.0%
! TAAGCTTGCTCAGCGCCATC+AGG - chr7.3:27820010-27820029 None:intergenic 55.0%
GGACCAGATGATGCTGCTGC+TGG + chr7.3:27819875-27819894 MS.gene51208:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 27819377 27820030 27819377 ID=MS.gene51208
chr7.3 mRNA 27819377 27820030 27819377 ID=MS.gene51208.t1;Parent=MS.gene51208
chr7.3 exon 27819377 27820030 27819377 ID=MS.gene51208.t1.exon1;Parent=MS.gene51208.t1
chr7.3 CDS 27819377 27820030 27819377 ID=cds.MS.gene51208.t1;Parent=MS.gene51208.t1
Gene Sequence

>MS.gene51208

ATGTCAAGCGATGATTACATGGTAGTTTTGGGAGCCATCAACTACAATTACAGGAGAAAAACTAGCTCGATTGACTTGGAGATTTTCTCATTGAGAGCTAATAAGTGGAAACAAGTTGAGGCTGCTTCTGATTTGCCTTATAGGAAGACTGCAAACTCTGAATATGGTCCCACTGTCGGGTCATTCTTGAATGATTCTATTCATTGGCTGGTTTATCATTATGAGACCAAAAGCAATGTTATTATTGCCTTTGATTTAAAGGAAAATACACTGTCAGAGATAGCTCTTCCAGATGACTTTTCTCGTGGAATTTATGTTTATGATTTGATGGTATTTGGCGGACTTATCAGTGTATGGAATATCGATTGGTCTAAAATTAAGATATGGGTGATGCAAGAATACAAAGTTCATTCATCTTGGTCTAAGACTCTTGAATTTTCTTTTTCTCCCGGTCTGGGTTTTTCCCCAATATGCTTTACAAGTTGTGGTGATATAGTTGGACCAGATGATGCTGCTGCTGGATTAGCGAAGCTTAATGACAAAGGACAACTGCTAGAGTATCACTCCTATGGCGCCTCTTACTATAAAAGATCCCAAACAACGGTGTATATAGAGTCTCTACTTTCACTCCCTGATGGCGCTGAGCAAGCTTAA
Protein sequence

>MS.gene51208.t1

MSSDDYMVVLGAINYNYRRKTSSIDLEIFSLRANKWKQVEAASDLPYRKTANSEYGPTVGSFLNDSIHWLVYHYETKSNVIIAFDLKENTLSEIALPDDFSRGIYVYDLMVFGGLISVWNIDWSKIKIWVMQEYKVHSSWSKTLEFSFSPGLGFSPICFTSCGDIVGPDDAAAGLAKLNDKGQLLEYHSYGASYYKRSQTTVYIESLLSLPDGAEQA