AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063472


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063472.t1 MTR_4g109280 68.852 183 52 3 2 182 180 359 5.56e-84 252
MS.gene063472.t1 MTR_6g060650 66.667 180 57 2 2 181 196 372 6.87e-82 247
MS.gene063472.t1 MTR_3g103020 64.516 186 58 4 2 182 196 378 3.39e-77 235
MS.gene063472.t1 MTR_4g117720 63.298 188 59 5 2 182 194 378 3.73e-74 228
MS.gene063472.t1 MTR_4g109270 61.413 184 66 3 2 182 183 364 1.02e-70 218
MS.gene063472.t1 MTR_3g034180 61.497 187 61 5 2 181 306 488 2.51e-70 221
MS.gene063472.t1 MTR_3g034130 62.644 174 55 4 2 168 192 362 9.77e-68 211
MS.gene063472.t1 MTR_4g117850 62.983 181 59 5 1 175 195 373 1.43e-65 205
MS.gene063472.t1 MTR_4g116320 59.783 184 63 6 1 179 78 255 1.34e-64 199
MS.gene063472.t1 MTR_4g118380 63.975 161 50 2 2 155 198 357 1.99e-64 202
MS.gene063472.t1 MTR_3g034160 60.428 187 63 5 2 181 192 374 8.06e-64 201
MS.gene063472.t1 MTR_4g094365 57.609 184 72 4 2 182 184 364 3.46e-62 196
MS.gene063472.t1 MTR_4g116330 58.378 185 70 4 2 182 199 380 3.68e-62 197
MS.gene063472.t1 MTR_4g122850 56.989 186 72 6 2 182 182 364 4.04e-61 194
MS.gene063472.t1 MTR_8g040935 56.383 188 74 4 1 182 23 208 1.85e-60 187
MS.gene063472.t1 MTR_4g118400 54.545 176 65 2 1 176 134 294 9.37e-59 186
MS.gene063472.t1 MTR_4g023130 56.831 183 69 5 2 181 137 312 1.13e-58 186
MS.gene063472.t1 MTR_8g433030 58.470 183 63 8 2 178 163 338 8.69e-58 184
MS.gene063472.t1 MTR_4g021785 54.348 184 77 4 1 182 177 355 1.92e-57 184
MS.gene063472.t1 MTR_8g032390 58.470 183 63 8 2 178 196 371 4.59e-57 183
MS.gene063472.t1 MTR_4g117780 54.891 184 80 2 2 182 184 367 1.43e-56 182
MS.gene063472.t1 MTR_4g023080 55.191 183 72 5 2 181 178 353 1.82e-56 181
MS.gene063472.t1 MTR_4g117770 54.348 184 81 2 2 182 184 367 6.93e-55 177
MS.gene063472.t1 MTR_4g117810 54.348 184 81 2 2 182 184 367 6.93e-55 177
MS.gene063472.t1 MTR_6g463430 51.913 183 80 3 2 182 26 202 2.55e-54 171
MS.gene063472.t1 MTR_4g023090 50.785 191 74 5 2 177 194 379 4.15e-53 174
MS.gene063472.t1 MTR_4g023070 50.256 195 76 6 2 181 181 369 5.82e-53 173
MS.gene063472.t1 MTR_1g055195 56.442 163 69 1 20 182 318 478 1.19e-52 175
MS.gene063472.t1 MTR_4g023380 49.758 207 72 6 2 182 179 379 2.65e-52 171
MS.gene063472.t1 MTR_1g055175 52.660 188 77 8 2 182 19 201 3.79e-52 166
MS.gene063472.t1 MTR_5g097210 55.556 180 73 5 1 177 183 358 4.07e-52 171
MS.gene063472.t1 MTR_5g070130 51.064 188 82 4 2 180 188 374 1.22e-51 170
MS.gene063472.t1 MTR_8g433040 53.514 185 67 7 2 182 195 364 1.93e-51 169
MS.gene063472.t1 MTR_8g032380 53.514 185 67 7 2 182 195 364 1.93e-51 169
MS.gene063472.t1 MTR_4g023190 53.226 186 72 7 2 180 180 357 2.27e-51 168
MS.gene063472.t1 MTR_4g023120 48.980 196 79 6 2 182 181 370 8.03e-51 167
MS.gene063472.t1 MTR_1g055165 56.287 167 64 6 20 182 157 318 2.71e-49 162
MS.gene063472.t1 MTR_5g070080 51.381 181 83 3 2 180 206 383 4.83e-49 163
MS.gene063472.t1 MTR_4g118360 63.492 126 42 2 59 182 3 126 2.40e-48 153
MS.gene063472.t1 MTR_8g102520 47.027 185 92 4 2 182 140 322 1.11e-47 158
MS.gene063472.t1 MTR_4g023150 51.075 186 77 7 2 180 180 358 1.37e-46 156
MS.gene063472.t1 MTR_4g023200 49.462 186 80 7 2 180 180 358 1.37e-46 156
MS.gene063472.t1 MTR_5g070088 46.703 182 94 2 1 180 203 383 1.98e-46 156
MS.gene063472.t1 MTR_4g118370 47.778 180 70 5 1 177 97 255 9.43e-46 151
MS.gene063472.t1 MTR_7g079400 44.444 189 95 4 2 182 151 337 2.15e-45 152
MS.gene063472.t1 MTR_4g117750 48.947 190 85 5 1 181 172 358 2.16e-45 155
MS.gene063472.t1 MTR_4g117750 61.468 109 40 1 74 180 373 481 1.78e-37 134
MS.gene063472.t1 MTR_1g055135 54.268 164 65 5 25 182 216 375 2.09e-43 148
MS.gene063472.t1 MTR_4g117870 46.703 182 58 5 2 182 124 267 2.83e-43 145
MS.gene063472.t1 MTR_4g116330 47.568 185 51 4 2 182 199 341 3.34e-41 142
MS.gene063472.t1 MTR_4g023440 43.646 181 61 3 2 182 193 332 1.16e-40 140
MS.gene063472.t1 MTR_7g079370 45.304 181 92 5 2 178 175 352 5.16e-40 139
MS.gene063472.t1 MTR_4g117530 42.541 181 70 3 2 182 107 253 7.44e-38 130
MS.gene063472.t1 MTR_5g023000 64.516 93 33 0 90 182 7 99 8.74e-38 125
MS.gene063472.t1 MTR_1g055250 46.591 176 78 10 1 169 87 253 2.75e-37 131
MS.gene063472.t1 MTR_8g098830 44.805 154 79 4 2 152 45 195 7.32e-37 127
MS.gene063472.t1 MTR_7g079450 47.887 142 67 3 42 182 2 137 7.22e-36 122
MS.gene063472.t1 MTR_4g122860 43.617 188 70 7 2 182 162 320 1.16e-34 124
MS.gene063472.t1 MTR_7g079420 40.642 187 96 7 5 182 964 1144 2.21e-34 128
MS.gene063472.t1 MTR_2g048580 36.898 187 100 7 2 182 168 342 1.19e-32 119
MS.gene063472.t1 MTR_4g114700 36.158 177 61 4 1 173 90 218 5.04e-29 107
MS.gene063472.t1 MTR_4g121580 41.667 180 68 8 1 175 195 342 9.94e-28 107
MS.gene063472.t1 MTR_7g117720 50.495 101 45 3 82 177 172 272 4.79e-22 90.1
MS.gene063472.t1 MTR_4g117430 57.647 85 32 2 2 86 75 155 5.49e-22 87.4
MS.gene063472.t1 MTR_4g023430 52.439 82 36 1 104 182 141 222 3.99e-21 86.7
MS.gene063472.t1 MTR_8g013010 58.537 82 27 3 2 83 235 309 6.62e-21 87.8
MS.gene063472.t1 MTR_4g122840 42.029 138 40 5 48 182 164 264 1.01e-19 84.0
MS.gene063472.t1 MTR_8g013005 59.322 59 24 0 124 182 1 59 4.13e-18 74.3
MS.gene063472.t1 MTR_4g109290 54.386 57 26 0 126 182 132 188 1.94e-13 65.9
MS.gene063472.t1 MTR_1g055145 48.000 75 35 2 25 98 131 202 1.01e-11 62.4
MS.gene063472.t1 MTR_3g010970 26.519 181 111 6 2 174 225 391 4.41e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATGACTTGTGGGTATT+TGG 0.144797 8.4:+18265931 MS.gene063472:CDS
TATTCATTAGCGTTCAAATA+AGG 0.220938 8.4:-18265751 None:intergenic
GGAGCAGGGTTCCTCTTTAA+TGG 0.253120 8.4:+18265781 MS.gene063472:CDS
GAGCAGGGTTCCTCTTTAAT+GGG 0.265091 8.4:+18265782 MS.gene063472:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.267319 8.4:+18265950 MS.gene063472:CDS
ACGAAGCTACAGTTGAAATA+TGG 0.272308 8.4:+18265980 MS.gene063472:CDS
ACAAAATGTGGTGATATTAT+TGG 0.310598 8.4:+18266099 MS.gene063472:CDS
ACTCTTGTCCTTCCTATTGA+TGG 0.321860 8.4:+18266042 MS.gene063472:CDS
TCTTTAATGGGGCTATTCAT+TGG 0.331439 8.4:+18265794 MS.gene063472:CDS
ACCCACAAGTCATAATAATT+AGG 0.339487 8.4:-18265925 None:intergenic
ATTCATTAGCGTTCAAATAA+GGG 0.368832 8.4:-18265750 None:intergenic
TCCACATGGATTGTTGAAAT+AGG 0.371891 8.4:-18266184 None:intergenic
TTGGCGAAGTACGATGAAAA+TGG 0.379685 8.4:+18266141 MS.gene063472:CDS
TAATGGGGCTATTCATTGGT+TGG 0.427301 8.4:+18265798 MS.gene063472:CDS
AGATAATATGTGGAAAGAGC+TGG 0.433933 8.4:+18265714 None:intergenic
ATTAAAGAGGAACCCTGCTC+CGG 0.440956 8.4:-18265779 None:intergenic
TGAGTTGGAATGCCATCAAT+AGG 0.444967 8.4:-18266054 None:intergenic
ATTGGAACAGATGGTGGTAA+TGG 0.447081 8.4:+18266117 MS.gene063472:CDS
ATCCTAATTATTATGACTTG+TGG 0.450172 8.4:+18265923 MS.gene063472:CDS
TCCTAATTATTATGACTTGT+GGG 0.453742 8.4:+18265924 MS.gene063472:CDS
CGAAGCTACAGTTGAAATAT+GGG 0.462892 8.4:+18265981 MS.gene063472:CDS
TGGAGAATTTCTCAGTCTAT+GGG 0.490268 8.4:+18265951 MS.gene063472:CDS
AATGAATATAATCCCGGAGC+AGG 0.490513 8.4:+18265766 MS.gene063472:CDS
AGTCATAATAATTAGGATCA+TGG 0.492245 8.4:-18265918 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.494278 8.4:+18266013 MS.gene063472:CDS
GAGACTCTGCTTTCACTCCC+TGG 0.504016 8.4:+18266228 MS.gene063472:CDS
TAAGCTTGCTCATTGCCACC+AGG 0.505650 8.4:-18266246 None:intergenic
CTCAGTATACATAGCCGCTC+GGG 0.506020 8.4:-18266208 None:intergenic
AACGCTAATGAATATAATCC+CGG 0.506207 8.4:+18265760 MS.gene063472:CDS
ATTTCTCAGTCTATGGGCTA+TGG 0.507502 8.4:+18265957 MS.gene063472:CDS
AATGAATAGCCCCATTAAAG+AGG 0.526680 8.4:-18265792 None:intergenic
TTAAAGAGGAACCCTGCTCC+GGG 0.544713 8.4:-18265778 None:intergenic
AATGGGTAGAAGTACTGAGT+TGG 0.583626 8.4:-18266069 None:intergenic
TAATATGTGGAAAGAGCTGG+AGG 0.586191 8.4:+18265717 None:intergenic
GGTGATATTATTGGAACAGA+TGG 0.588464 8.4:+18266108 MS.gene063472:CDS
TCTCAGTATACATAGCCGCT+CGG 0.591276 8.4:-18266209 None:intergenic
GATATTATTGGAACAGATGG+TGG 0.593108 8.4:+18266111 MS.gene063472:CDS
AACAGATGGTGGTAATGGAT+TGG 0.598103 8.4:+18266122 MS.gene063472:CDS
AGCAGGGTTCCTCTTTAATG+GGG 0.611689 8.4:+18265783 MS.gene063472:CDS
TTGGAATGCCATCAATAGGA+AGG 0.614361 8.4:-18266050 None:intergenic
TCCTATTTCAACAATCCATG+TGG 0.619417 8.4:+18266183 MS.gene063472:CDS
AAGCTTGCTCATTGCCACCA+GGG 0.620136 8.4:-18266245 None:intergenic
ATGAATATAATCCCGGAGCA+GGG 0.652117 8.4:+18265767 MS.gene063472:CDS
ATAGCCGCTCGGGATCCACA+TGG 0.663607 8.4:-18266198 None:intergenic
ACTCTGCTTTCACTCCCTGG+TGG 0.675119 8.4:+18266231 MS.gene063472:CDS
CCATTGTGCTGTACAAAATG+TGG 0.678555 8.4:+18266087 MS.gene063472:CDS
CAATCCATGTGGATCCCGAG+CGG 0.707972 8.4:+18266194 MS.gene063472:CDS
AATATGTGGAAAGAGCTGGA+GGG 0.747768 8.4:+18265718 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCTTTTCTATTAAATCAA+AGG - chr8.4:18265860-18265879 None:intergenic 15.0%
! ACAAAATGTGGTGATATTAT+TGG + chr8.4:18266099-18266118 MS.gene063472:CDS 25.0%
! AGTCATAATAATTAGGATCA+TGG - chr8.4:18265921-18265940 None:intergenic 25.0%
! ATCCTAATTATTATGACTTG+TGG + chr8.4:18265923-18265942 MS.gene063472:CDS 25.0%
! ATTCATTAGCGTTCAAATAA+GGG - chr8.4:18265753-18265772 None:intergenic 25.0%
! TATTCATTAGCGTTCAAATA+AGG - chr8.4:18265754-18265773 None:intergenic 25.0%
! TGGTCAAAAATAAAATCATC+CGG - chr8.4:18265901-18265920 None:intergenic 25.0%
!! ACTTTTAGATATACATTTGC+CGG + chr8.4:18265879-18265898 MS.gene063472:CDS 25.0%
!! TCCTAATTATTATGACTTGT+GGG + chr8.4:18265924-18265943 MS.gene063472:CDS 25.0%
AACGCTAATGAATATAATCC+CGG + chr8.4:18265760-18265779 MS.gene063472:CDS 30.0%
ACCCACAAGTCATAATAATT+AGG - chr8.4:18265928-18265947 None:intergenic 30.0%
! TATTATGACTTGTGGGTATT+TGG + chr8.4:18265931-18265950 MS.gene063472:CDS 30.0%
AATACAAAGTGCATTCGTCT+TGG + chr8.4:18266013-18266032 MS.gene063472:CDS 35.0%
AATGAATAGCCCCATTAAAG+AGG - chr8.4:18265795-18265814 None:intergenic 35.0%
ACGAAGCTACAGTTGAAATA+TGG + chr8.4:18265980-18265999 MS.gene063472:CDS 35.0%
CGAAGCTACAGTTGAAATAT+GGG + chr8.4:18265981-18266000 MS.gene063472:CDS 35.0%
GATATTATTGGAACAGATGG+TGG + chr8.4:18266111-18266130 MS.gene063472:CDS 35.0%
GGTGATATTATTGGAACAGA+TGG + chr8.4:18266108-18266127 MS.gene063472:CDS 35.0%
TCCTATTTCAACAATCCATG+TGG + chr8.4:18266183-18266202 MS.gene063472:CDS 35.0%
TCTTTAATGGGGCTATTCAT+TGG + chr8.4:18265794-18265813 MS.gene063472:CDS 35.0%
TGGAGAATTTCTCAGTCTAT+GGG + chr8.4:18265951-18265970 MS.gene063472:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG + chr8.4:18265950-18265969 MS.gene063472:CDS 35.0%
! CACATTTTGTACAGCACAAT+GGG - chr8.4:18266089-18266108 None:intergenic 35.0%
! TCCACATGGATTGTTGAAAT+AGG - chr8.4:18266187-18266206 None:intergenic 35.0%
AATGAATATAATCCCGGAGC+AGG + chr8.4:18265766-18265785 MS.gene063472:CDS 40.0%
ACTCTTGTCCTTCCTATTGA+TGG + chr8.4:18266042-18266061 MS.gene063472:CDS 40.0%
ATGAATATAATCCCGGAGCA+GGG + chr8.4:18265767-18265786 MS.gene063472:CDS 40.0%
CCATTGTGCTGTACAAAATG+TGG + chr8.4:18266087-18266106 MS.gene063472:CDS 40.0%
TAATGGGGCTATTCATTGGT+TGG + chr8.4:18265798-18265817 MS.gene063472:CDS 40.0%
TTGGAATGCCATCAATAGGA+AGG - chr8.4:18266053-18266072 None:intergenic 40.0%
TTGGCGAAGTACGATGAAAA+TGG + chr8.4:18266141-18266160 MS.gene063472:CDS 40.0%
! ATTGGAACAGATGGTGGTAA+TGG + chr8.4:18266117-18266136 MS.gene063472:CDS 40.0%
! ATTTCTCAGTCTATGGGCTA+TGG + chr8.4:18265957-18265976 MS.gene063472:CDS 40.0%
! CCACATTTTGTACAGCACAA+TGG - chr8.4:18266090-18266109 None:intergenic 40.0%
! TGAGTTGGAATGCCATCAAT+AGG - chr8.4:18266057-18266076 None:intergenic 40.0%
!! AACAGATGGTGGTAATGGAT+TGG + chr8.4:18266122-18266141 MS.gene063472:CDS 40.0%
!! AATGGGTAGAAGTACTGAGT+TGG - chr8.4:18266072-18266091 None:intergenic 40.0%
AGCAGGGTTCCTCTTTAATG+GGG + chr8.4:18265783-18265802 MS.gene063472:CDS 45.0%
ATTAAAGAGGAACCCTGCTC+CGG - chr8.4:18265782-18265801 None:intergenic 45.0%
GAGCAGGGTTCCTCTTTAAT+GGG + chr8.4:18265782-18265801 MS.gene063472:CDS 45.0%
TCTCAGTATACATAGCCGCT+CGG - chr8.4:18266212-18266231 None:intergenic 45.0%
CTCAGTATACATAGCCGCTC+GGG - chr8.4:18266211-18266230 None:intergenic 50.0%
GGAGCAGGGTTCCTCTTTAA+TGG + chr8.4:18265781-18265800 MS.gene063472:CDS 50.0%
TTAAAGAGGAACCCTGCTCC+GGG - chr8.4:18265781-18265800 None:intergenic 50.0%
! AAGCTTGCTCATTGCCACCA+GGG - chr8.4:18266248-18266267 None:intergenic 50.0%
! TAAGCTTGCTCATTGCCACC+AGG - chr8.4:18266249-18266268 None:intergenic 50.0%
ACTCTGCTTTCACTCCCTGG+TGG + chr8.4:18266231-18266250 MS.gene063472:CDS 55.0%
CAATCCATGTGGATCCCGAG+CGG + chr8.4:18266194-18266213 MS.gene063472:CDS 55.0%
GAGACTCTGCTTTCACTCCC+TGG + chr8.4:18266228-18266247 MS.gene063472:CDS 55.0%
ATAGCCGCTCGGGATCCACA+TGG - chr8.4:18266201-18266220 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 18265721 18266269 18265721 ID=MS.gene063472
chr8.4 mRNA 18265721 18266269 18265721 ID=MS.gene063472.t1;Parent=MS.gene063472
chr8.4 exon 18265721 18266269 18265721 ID=MS.gene063472.t1.exon1;Parent=MS.gene063472.t1
chr8.4 CDS 18265721 18266269 18265721 ID=cds.MS.gene063472.t1;Parent=MS.gene063472.t1
Gene Sequence

>MS.gene063472

ATGTGGAAAGAGCTGGAGGGTACTCACTTCCCTTATTTGAACGCTAATGAATATAATCCCGGAGCAGGGTTCCTCTTTAATGGGGCTATTCATTGGTTGGCTTTTAGTTGTGATCTACTATATGATGTTATTGTTGCCTTTGATTTAATAGAAAAGAAACTTTTAGATATACATTTGCCGGATGATTTTATTTTTGACCATGATCCTAATTATTATGACTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGGCTATGGACGAAGCTACAGTTGAAATATGGGTTATGAAAGAATACAAAGTGCATTCGTCTTGGACTAAGACTCTTGTCCTTCCTATTGATGGCATTCCAACTCAGTACTTCTACCCATTGTGCTGTACAAAATGTGGTGATATTATTGGAACAGATGGTGGTAATGGATTGGCGAAGTACGATGAAAATGGAGAATTACTAGACGTTCATTCCTATTTCAACAATCCATGTGGATCCCGAGCGGCTATGTATACTGAGACTCTGCTTTCACTCCCTGGTGGCAATGAGCAAGCTTAA

Protein sequence

>MS.gene063472.t1

MWKELEGTHFPYLNANEYNPGAGFLFNGAIHWLAFSCDLLYDVIVAFDLIEKKLLDIHLPDDFIFDHDPNYYDLWVFGEFLSLWAMDEATVEIWVMKEYKVHSSWTKTLVLPIDGIPTQYFYPLCCTKCGDIIGTDGGNGLAKYDENGELLDVHSYFNNPCGSRAAMYTETLLSLPGGNEQA