AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87557

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87557.t1 MTR_4g023120 91.698 265 22 0 1 265 106 370 0.0 508
MS.gene87557.t1 MTR_4g023070 90.909 264 24 0 1 264 106 369 1.07e-180 501
MS.gene87557.t1 MTR_4g023090 60.662 272 95 4 1 265 120 386 1.87e-109 321
MS.gene87557.t1 MTR_4g021785 61.423 267 78 4 1 265 112 355 2.43e-108 317
MS.gene87557.t1 MTR_4g023130 63.019 265 74 4 1 264 71 312 1.48e-107 314
MS.gene87557.t1 MTR_4g023080 61.887 265 77 4 1 264 112 353 2.69e-105 310
MS.gene87557.t1 MTR_4g023380 57.534 292 65 5 1 265 120 379 2.47e-104 308
MS.gene87557.t1 MTR_4g023150 55.639 266 95 5 1 263 113 358 7.72e-90 270
MS.gene87557.t1 MTR_4g023200 55.263 266 96 5 1 263 113 358 2.82e-89 269
MS.gene87557.t1 MTR_4g023190 56.015 266 93 6 1 263 113 357 1.04e-88 267
MS.gene87557.t1 MTR_3g103020 55.351 271 90 9 6 265 128 378 3.15e-85 259
MS.gene87557.t1 MTR_4g023440 51.698 265 73 3 1 265 123 332 3.59e-81 247
MS.gene87557.t1 MTR_4g117720 52.158 278 97 11 1 262 118 375 1.71e-79 244
MS.gene87557.t1 MTR_4g094365 52.190 274 102 8 1 265 111 364 4.21e-79 243
MS.gene87557.t1 MTR_4g116330 51.685 267 106 7 1 262 129 377 9.08e-79 243
MS.gene87557.t1 MTR_6g060650 50.000 272 109 8 1 264 120 372 2.06e-77 239
MS.gene87557.t1 MTR_4g117850 51.119 268 100 9 6 262 129 376 6.03e-75 233
MS.gene87557.t1 MTR_4g109280 49.815 271 112 7 1 265 107 359 4.88e-72 225
MS.gene87557.t1 MTR_8g032390 52.273 264 99 10 6 260 125 370 1.53e-71 224
MS.gene87557.t1 MTR_4g109270 49.071 269 105 8 6 265 119 364 1.75e-69 218
MS.gene87557.t1 MTR_3g034160 51.471 272 99 10 6 262 119 372 2.61e-69 218
MS.gene87557.t1 MTR_3g034180 49.817 273 108 9 6 265 233 489 3.33e-69 221
MS.gene87557.t1 MTR_4g117780 49.071 269 109 7 6 265 118 367 1.11e-68 216
MS.gene87557.t1 MTR_3g034130 51.373 255 92 9 6 246 119 355 2.78e-68 216
MS.gene87557.t1 MTR_8g433040 48.134 268 104 7 6 265 124 364 6.40e-67 212
MS.gene87557.t1 MTR_8g032380 48.134 268 104 7 6 265 124 364 6.40e-67 212
MS.gene87557.t1 MTR_8g433030 54.545 231 82 8 35 260 125 337 7.54e-66 208
MS.gene87557.t1 MTR_4g117770 47.810 274 115 7 1 265 113 367 1.01e-65 209
MS.gene87557.t1 MTR_4g117810 47.810 274 115 7 1 265 113 367 1.01e-65 209
MS.gene87557.t1 MTR_4g122850 49.635 274 104 13 6 265 111 364 1.17e-65 209
MS.gene87557.t1 MTR_5g097210 49.242 264 103 11 8 262 119 360 2.62e-64 206
MS.gene87557.t1 MTR_4g118380 48.031 254 100 7 1 240 122 357 3.06e-64 205
MS.gene87557.t1 MTR_4g117870 46.388 263 87 9 6 265 56 267 2.45e-63 199
MS.gene87557.t1 MTR_4g116320 51.948 231 86 6 39 262 43 255 6.25e-62 196
MS.gene87557.t1 MTR_6g463430 55.122 205 71 4 65 265 15 202 1.00e-61 193
MS.gene87557.t1 MTR_5g070080 47.212 269 107 10 4 260 135 380 1.54e-59 193
MS.gene87557.t1 MTR_1g055135 45.588 272 118 9 6 265 122 375 1.98e-59 193
MS.gene87557.t1 MTR_8g040935 51.429 210 81 5 64 265 12 208 5.36e-59 186
MS.gene87557.t1 MTR_5g070130 43.396 265 121 7 7 258 121 369 6.64e-57 187
MS.gene87557.t1 MTR_4g116330 43.820 267 88 7 1 262 129 338 6.68e-57 185
MS.gene87557.t1 MTR_1g055195 42.336 274 117 8 1 262 231 475 2.17e-56 188
MS.gene87557.t1 MTR_5g070088 43.511 262 130 6 4 260 132 380 4.20e-56 184
MS.gene87557.t1 MTR_4g118400 43.511 262 100 8 1 258 76 293 5.76e-56 182
MS.gene87557.t1 MTR_4g118370 42.366 262 107 10 8 262 33 257 3.51e-54 176
MS.gene87557.t1 MTR_4g121580 44.981 269 93 11 6 264 125 348 3.58e-53 177
MS.gene87557.t1 MTR_4g117750 44.238 269 118 9 6 261 106 355 3.19e-51 174
MS.gene87557.t1 MTR_4g117750 47.917 144 54 3 129 263 350 481 6.04e-28 112
MS.gene87557.t1 MTR_7g079400 40.441 272 131 9 7 265 84 337 7.33e-49 164
MS.gene87557.t1 MTR_7g079370 40.892 269 125 9 8 258 97 349 4.53e-45 155
MS.gene87557.t1 MTR_1g055250 40.650 246 114 9 31 265 46 270 1.36e-43 150
MS.gene87557.t1 MTR_1g055175 43.836 219 92 8 58 264 1 200 1.45e-43 147
MS.gene87557.t1 MTR_8g102520 38.889 270 136 10 6 265 72 322 1.84e-43 150
MS.gene87557.t1 MTR_4g114700 38.806 268 89 7 1 256 14 218 2.62e-42 145
MS.gene87557.t1 MTR_4g117530 41.277 235 84 6 32 265 72 253 4.48e-42 144
MS.gene87557.t1 MTR_1g055165 38.258 264 90 8 8 265 122 318 6.95e-40 140
MS.gene87557.t1 MTR_4g117430 50.993 151 59 6 7 154 14 152 4.56e-38 131
MS.gene87557.t1 MTR_8g013010 46.914 162 67 6 1 154 159 309 2.38e-34 126
MS.gene87557.t1 MTR_4g023430 49.242 132 35 4 142 265 115 222 3.24e-29 110
MS.gene87557.t1 MTR_2g048580 33.585 265 125 11 3 258 113 335 2.33e-28 110
MS.gene87557.t1 MTR_7g079420 34.191 272 119 12 7 263 917 1143 2.92e-28 114
MS.gene87557.t1 MTR_4g122860 38.835 206 73 9 71 265 157 320 4.28e-28 110
MS.gene87557.t1 MTR_5g023000 53.636 110 37 2 158 265 2 99 9.66e-28 102
MS.gene87557.t1 MTR_4g118360 51.200 125 41 4 142 262 15 123 1.10e-27 103
MS.gene87557.t1 MTR_4g116340 46.457 127 57 4 1 119 124 247 1.97e-26 103
MS.gene87557.t1 MTR_8g098830 38.764 178 84 8 66 236 35 194 9.51e-25 99.0
MS.gene87557.t1 MTR_7g079450 39.103 156 73 6 113 265 1 137 2.32e-22 90.1
MS.gene87557.t1 MTR_4g023100 46.087 115 55 2 157 265 6 119 4.39e-21 86.3
MS.gene87557.t1 MTR_7g117720 47.541 122 45 4 143 258 162 270 4.58e-21 90.1
MS.gene87557.t1 MTR_7g117720 52.941 85 34 2 6 86 102 184 5.72e-18 81.6
MS.gene87557.t1 MTR_6g034720 48.485 99 49 2 33 130 72 169 8.69e-21 88.2
MS.gene87557.t1 MTR_2g028140 29.825 228 125 10 2 205 122 338 4.12e-19 86.3
MS.gene87557.t1 MTR_4g133170 30.909 220 119 9 6 204 104 311 4.51e-19 86.3
MS.gene87557.t1 MTR_2g028130 28.507 221 130 9 1 200 120 333 2.11e-18 84.3
MS.gene87557.t1 MTR_1g094210 25.847 236 142 7 7 237 170 377 4.02e-18 83.6
MS.gene87557.t1 MTR_2g021050 27.381 252 142 9 22 261 119 341 5.50e-18 82.8
MS.gene87557.t1 MTR_0667s0010 29.148 223 125 10 3 200 110 324 5.61e-18 83.2
MS.gene87557.t1 MTR_1g094210 25.591 254 161 7 7 257 170 398 5.99e-18 82.8
MS.gene87557.t1 MTR_2g025960 29.204 226 121 11 2 200 20 233 8.20e-18 81.6
MS.gene87557.t1 MTR_4g070550 30.542 203 110 9 2 180 116 311 4.23e-17 80.5
MS.gene87557.t1 MTR_1g068875 31.429 175 108 7 33 200 139 308 1.06e-16 79.3
MS.gene87557.t1 MTR_1g097560 24.907 269 153 11 1 257 142 373 1.08e-16 79.3
MS.gene87557.t1 MTR_8g013005 63.793 58 19 1 209 264 1 58 2.12e-16 72.0
MS.gene87557.t1 MTR_5g086590 29.851 201 126 8 2 193 9 203 2.33e-16 76.6
MS.gene87557.t1 MTR_2g426070 29.954 217 129 8 2 200 120 331 2.64e-16 78.2
MS.gene87557.t1 MTR_2g426020 29.954 217 130 7 1 201 114 324 5.61e-16 77.4
MS.gene87557.t1 MTR_2g028160 29.817 218 124 10 5 201 127 336 1.14e-15 76.6
MS.gene87557.t1 MTR_1g068835 27.016 248 153 10 1 234 99 332 1.67e-15 75.9
MS.gene87557.t1 MTR_5g097350 24.427 262 164 8 7 258 146 383 2.75e-15 75.1
MS.gene87557.t1 MTR_1g068860 30.093 216 120 11 7 200 105 311 3.01e-15 75.1
MS.gene87557.t1 MTR_3g083010 27.803 223 131 9 2 200 123 339 4.28e-15 74.7
MS.gene87557.t1 MTR_3g009310 27.237 257 159 9 6 257 157 390 6.05e-15 74.3
MS.gene87557.t1 MTR_8g101580 25.483 259 167 9 6 257 155 394 6.88e-15 73.9
MS.gene87557.t1 MTR_4g055060 30.732 205 120 9 1 194 138 331 1.27e-14 73.2
MS.gene87557.t1 MTR_2g024110 27.488 211 129 8 7 200 108 311 1.93e-14 72.8
MS.gene87557.t1 MTR_3g023930 28.030 264 149 11 1 257 138 367 1.99e-14 72.4
MS.gene87557.t1 MTR_2g425980 26.923 286 155 12 2 256 113 375 2.89e-14 72.4
MS.gene87557.t1 MTR_2g426090 26.852 216 137 7 2 199 115 327 4.03e-14 72.0
MS.gene87557.t1 MTR_2g046920 30.093 216 128 11 7 217 137 334 4.51e-14 71.6
MS.gene87557.t1 MTR_1g097490 24.803 254 167 7 6 257 159 390 5.21e-14 71.6
MS.gene87557.t1 MTR_4g117550 25.614 285 139 11 5 263 137 374 5.23e-14 71.2
MS.gene87557.t1 MTR_2g021155 30.198 202 125 8 9 201 147 341 5.33e-14 72.0
MS.gene87557.t1 MTR_0129s0050 27.000 300 155 11 6 262 130 408 6.53e-14 71.2
MS.gene87557.t1 MTR_7g070830 28.099 242 133 10 6 218 146 375 8.37e-14 70.9
MS.gene87557.t1 MTR_5g097330 26.909 275 164 9 7 262 147 403 9.29e-14 70.9
MS.gene87557.t1 MTR_7g017130 29.612 206 121 11 4 189 137 338 1.73e-13 70.1
MS.gene87557.t1 MTR_7g017170 29.612 206 121 11 4 189 137 338 1.97e-13 69.7
MS.gene87557.t1 MTR_3g010920 27.451 255 149 11 8 257 158 381 2.01e-13 69.7
MS.gene87557.t1 MTR_2g028110 24.138 232 156 5 31 259 145 359 2.82e-13 69.3
MS.gene87557.t1 MTR_7g009265 29.004 231 134 10 10 218 58 280 4.17e-13 68.6
MS.gene87557.t1 MTR_7g079640 27.237 257 148 11 9 253 136 365 8.40e-13 67.8
MS.gene87557.t1 MTR_7g070880 27.273 242 135 10 6 218 146 375 9.21e-13 67.8
MS.gene87557.t1 MTR_2g104340 25.597 293 164 11 6 264 126 398 1.48e-12 67.4
MS.gene87557.t1 MTR_2g022000 26.715 277 154 12 9 254 149 407 1.82e-12 67.0
MS.gene87557.t1 MTR_2g021150 27.778 216 135 9 7 218 130 328 1.82e-12 67.0
MS.gene87557.t1 MTR_5g064700 22.145 289 172 10 9 262 98 368 1.87e-12 66.6
MS.gene87557.t1 MTR_6g065510 26.293 232 131 10 9 218 76 289 2.38e-12 66.2
MS.gene87557.t1 MTR_1g097330 25.877 228 145 7 9 230 127 336 3.09e-12 66.2
MS.gene87557.t1 MTR_7g034310 27.049 244 129 12 34 259 17 229 3.89e-12 64.7
MS.gene87557.t1 MTR_0129s0070 23.776 286 177 9 3 265 132 399 4.41e-12 65.9
MS.gene87557.t1 MTR_4g019990 25.991 227 140 8 7 218 131 344 4.56e-12 65.9
MS.gene87557.t1 MTR_2g031720 25.725 276 176 8 7 265 110 373 6.54e-12 65.5
MS.gene87557.t1 MTR_3g014540 30.303 264 151 15 7 260 140 380 1.09e-11 64.7
MS.gene87557.t1 MTR_2g078870 25.746 268 161 14 1 257 67 307 1.42e-11 63.9
MS.gene87557.t1 MTR_5g097680 23.693 287 170 9 9 263 159 428 1.45e-11 64.3
MS.gene87557.t1 MTR_4g109290 64.151 53 17 1 215 265 136 188 1.50e-11 62.4
MS.gene87557.t1 MTR_2g021800 25.000 280 163 9 9 258 150 412 1.64e-11 64.3
MS.gene87557.t1 MTR_2g021770 26.855 283 160 10 10 262 142 407 1.74e-11 63.9
MS.gene87557.t1 MTR_3g013740 27.969 261 159 12 7 257 165 406 2.23e-11 63.9
MS.gene87557.t1 MTR_2g461450 26.230 183 122 5 2 180 118 291 2.27e-11 63.5
MS.gene87557.t1 MTR_2g026010 30.000 220 121 11 6 200 104 315 2.58e-11 63.5
MS.gene87557.t1 MTR_7g057270 26.971 241 140 10 2 218 142 370 3.16e-11 63.2
MS.gene87557.t1 MTR_7g055953 27.149 221 117 10 34 232 90 288 3.75e-11 62.8
MS.gene87557.t1 MTR_3g009300 27.895 190 120 7 9 193 121 298 4.42e-11 62.8
MS.gene87557.t1 MTR_2g046860 25.114 219 142 10 7 218 25 228 4.43e-11 62.4
MS.gene87557.t1 MTR_3g036290 27.344 256 150 12 6 257 133 356 5.44e-11 62.4
MS.gene87557.t1 MTR_5g057710 29.717 212 123 10 1 207 155 345 6.35e-11 62.4
MS.gene87557.t1 MTR_8g467140 28.342 187 116 9 9 187 144 320 8.70e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87557.t1 AT3G06240 27.099 262 156 8 7 257 182 419 2.11e-14 72.8

Find 56 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGAATGGATTAACTTTAT+TGG 0.141488 4.1:+75306267 None:intergenic
ACAGAAAATGGTGATATTAT+TGG 0.164193 4.1:-75305785 MS.gene87557:CDS
CATTCTGGTTTGTGGGTATT+TGG 0.166612 4.1:-75305995 MS.gene87557:CDS
GAAGCGTATATTAATCTATA+TGG 0.223056 4.1:-75306337 MS.gene87557:CDS
GTAATGATACAATTGAAATA+TGG 0.247553 4.1:-75305940 MS.gene87557:CDS
CCCATGTATTCTACAGAAAA+TGG 0.267417 4.1:-75305797 MS.gene87557:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.269491 4.1:-75305976 MS.gene87557:CDS
CTTCCTCAGCATGTCATTCC+TGG 0.289129 4.1:-75305869 MS.gene87557:CDS
CATTGTTGGCTTTGATTTAA+CGG 0.305680 4.1:-75306074 MS.gene87557:CDS
CACAGAGCATTAGTACATTC+TGG 0.320173 4.1:-75306010 MS.gene87557:CDS
GGTGATATTATTGGAAAATA+TGG 0.325508 4.1:-75305776 MS.gene87557:CDS
TTCGCGTCGTGATTTAGTTT+TGG 0.338685 4.1:-75306101 MS.gene87557:CDS
GTGAAAGTAGTCCGCAAATC+TGG 0.357133 4.1:+75305820 None:intergenic
GGATTCCATATGTATATGTT+TGG 0.378052 4.1:+75306406 None:intergenic
GAGGGTAATCACTTACCTTA+TGG 0.385311 4.1:-75306181 MS.gene87557:CDS
TATATTAATCTATATGGGTT+TGG 0.387173 4.1:-75306331 MS.gene87557:CDS
AGTCTATGGGTTAAGGATAC+TGG 0.398340 4.1:-75305962 MS.gene87557:CDS
TGGTGTTCCTCAATCACATT+TGG 0.406622 4.1:-75306242 MS.gene87557:CDS
ATTTCTCAGTCTATGGGTTA+AGG 0.409114 4.1:-75305969 MS.gene87557:CDS
GCTCTGTGATCAACATCATT+TGG 0.411538 4.1:+75306025 None:intergenic
AAAGTTAATCCATTCTTAGT+TGG 0.417793 4.1:-75306262 MS.gene87557:CDS
TAATGATACAATTGAAATAT+GGG 0.423786 4.1:-75305939 MS.gene87557:CDS
ATTAGTACATTCTGGTTTGT+GGG 0.428082 4.1:-75306002 MS.gene87557:CDS
CATTAGTACATTCTGGTTTG+TGG 0.428815 4.1:-75306003 MS.gene87557:CDS
AAGCGTATATTAATCTATAT+GGG 0.430207 4.1:-75306336 MS.gene87557:CDS
GTCATCCAAACATATACATA+TGG 0.469389 4.1:-75306411 MS.gene87557:CDS
TCTCGTTTAGAGACAATACT+TGG 0.472530 4.1:-75306213 MS.gene87557:CDS
TGATCAACATCATTTGGAAC+AGG 0.489346 4.1:+75306031 None:intergenic
TCTCTATAATCGTCGCCATA+AGG 0.490230 4.1:+75306166 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.494278 4.1:-75305907 MS.gene87557:CDS
TGGAGAATTTCTCAGTCTAT+GGG 0.497173 4.1:-75305975 MS.gene87557:CDS
TGAGGAACACCAACTAAGAA+TGG 0.498401 4.1:+75306253 None:intergenic
ATATAGATTAATATACGCTT+CGG 0.513373 4.1:+75306339 None:intergenic
AGAGAGTGAAAGTAGCGATT+CGG 0.530986 4.1:+75305664 None:intergenic
GAATCGCTACTTTCACTCTC+TGG 0.536742 4.1:-75305662 MS.gene87557:CDS
AAAATATGGTGGTACTAGAT+TGG 0.540379 4.1:-75305762 MS.gene87557:CDS
TCTGTACTATTCCAGATTTG+CGG 0.550677 4.1:-75305831 MS.gene87557:CDS
ACGATTATAGAGAAGGAGAA+GGG 0.565105 4.1:-75306156 MS.gene87557:CDS
TTCGGTATACACGACAACTT+CGG 0.573602 4.1:+75305682 None:intergenic
TTATAGAGAAGGAGAAGGGG+TGG 0.585026 4.1:-75306152 MS.gene87557:CDS
TTGGCTTTGATTTAACGGAA+AGG 0.585773 4.1:-75306069 MS.gene87557:CDS
GACGATTATAGAGAAGGAGA+AGG 0.587454 4.1:-75306157 MS.gene87557:CDS
TATGGCGACGATTATAGAGA+AGG 0.601035 4.1:-75306163 MS.gene87557:CDS
GTCTAGAGATGATTATGTGG+TGG 0.603968 4.1:-75306299 MS.gene87557:CDS
TACATATGGAATCCATCCAC+CGG 0.605701 4.1:-75306397 MS.gene87557:CDS
CAAATCTGGAATAGTACAGA+GGG 0.610389 4.1:+75305834 None:intergenic
GCAAATCTGGAATAGTACAG+AGG 0.612314 4.1:+75305833 None:intergenic
CAATACTTGGAAAGAAATCG+AGG 0.619297 4.1:-75306200 MS.gene87557:CDS
CGATTATAGAGAAGGAGAAG+GGG 0.628339 4.1:-75306155 MS.gene87557:CDS
GTATACACGACAACTTCGGA+TGG 0.629693 4.1:+75305686 None:intergenic
TGACCAGGAATGACATGCTG+AGG 0.635899 4.1:+75305866 None:intergenic
TTGGTGAAGTATAATGACAA+AGG 0.639076 4.1:-75305743 MS.gene87557:CDS
GATATTATTGGAAAATATGG+TGG 0.639099 4.1:-75305773 MS.gene87557:CDS
AGAGGGTTAAAGTAGTGACC+AGG 0.655673 4.1:+75305851 None:intergenic
AATACTTGGAAAGAAATCGA+GGG 0.666838 4.1:-75306199 MS.gene87557:CDS
TCAGTCTAGAGATGATTATG+TGG 0.684477 4.1:-75306302 MS.gene87557:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGATACAATTGAAATAT+GGG - chr4.1:75306122-75306141 MS.gene87557:CDS 15.0%
!! AAGCGTATATTAATCTATAT+GGG - chr4.1:75305725-75305744 MS.gene87557:CDS 20.0%
!! ATATAGATTAATATACGCTT+CGG + chr4.1:75305725-75305744 None:intergenic 20.0%
!! GTAATGATACAATTGAAATA+TGG - chr4.1:75306121-75306140 MS.gene87557:CDS 20.0%
!! TATATTAATCTATATGGGTT+TGG - chr4.1:75305730-75305749 MS.gene87557:CDS 20.0%
!!! TAAGAATGGATTAACTTTAT+TGG + chr4.1:75305797-75305816 None:intergenic 20.0%
! AAAGTTAATCCATTCTTAGT+TGG - chr4.1:75305799-75305818 MS.gene87557:CDS 25.0%
! ACAGAAAATGGTGATATTAT+TGG - chr4.1:75306276-75306295 MS.gene87557:CDS 25.0%
! GAAGCGTATATTAATCTATA+TGG - chr4.1:75305724-75305743 MS.gene87557:CDS 25.0%
! GATATTATTGGAAAATATGG+TGG - chr4.1:75306288-75306307 MS.gene87557:CDS 25.0%
! GGTGATATTATTGGAAAATA+TGG - chr4.1:75306285-75306304 MS.gene87557:CDS 25.0%
!! AAGTATTTGTCTTTTTGATC+CGG + chr4.1:75305686-75305705 None:intergenic 25.0%
AAAAACTCCAAATGTGATTG+AGG + chr4.1:75305829-75305848 None:intergenic 30.0%
AAAATATGGTGGTACTAGAT+TGG - chr4.1:75306299-75306318 MS.gene87557:CDS 30.0%
AATACTTGGAAAGAAATCGA+GGG - chr4.1:75305862-75305881 MS.gene87557:CDS 30.0%
GGATTCCATATGTATATGTT+TGG + chr4.1:75305658-75305677 None:intergenic 30.0%
GTCATCCAAACATATACATA+TGG - chr4.1:75305650-75305669 MS.gene87557:CDS 30.0%
TTGGTGAAGTATAATGACAA+AGG - chr4.1:75306318-75306337 MS.gene87557:CDS 30.0%
! AATGACAAAGGACAGTTTTT+AGG - chr4.1:75306330-75306349 MS.gene87557:CDS 30.0%
! ACCATTTTCTGTAGAATACA+TGG + chr4.1:75306268-75306287 None:intergenic 30.0%
! ATGACAAAGGACAGTTTTTA+GGG - chr4.1:75306331-75306350 MS.gene87557:CDS 30.0%
! CATTTTCTGTAGAATACATG+GGG + chr4.1:75306266-75306285 None:intergenic 30.0%
! CCATTTTCTGTAGAATACAT+GGG + chr4.1:75306267-75306286 None:intergenic 30.0%
! TTTTTAATGGGGCTATTCAT+TGG - chr4.1:75305932-75305951 MS.gene87557:CDS 30.0%
!! ATTAGTACATTCTGGTTTGT+GGG - chr4.1:75306059-75306078 MS.gene87557:CDS 30.0%
!! CATTGTTGGCTTTGATTTAA+CGG - chr4.1:75305987-75306006 MS.gene87557:CDS 30.0%
!!! TTAGTTTTGGATGTCATTGT+TGG - chr4.1:75305973-75305992 MS.gene87557:CDS 30.0%
AATACAAAGTGCATTCGTCT+TGG - chr4.1:75306154-75306173 MS.gene87557:CDS 35.0%
ACGATTATAGAGAAGGAGAA+GGG - chr4.1:75305905-75305924 MS.gene87557:CDS 35.0%
CAATACTTGGAAAGAAATCG+AGG - chr4.1:75305861-75305880 MS.gene87557:CDS 35.0%
CCCATGTATTCTACAGAAAA+TGG - chr4.1:75306264-75306283 MS.gene87557:CDS 35.0%
TCAGTCTAGAGATGATTATG+TGG - chr4.1:75305759-75305778 MS.gene87557:CDS 35.0%
TCTCGTTTAGAGACAATACT+TGG - chr4.1:75305848-75305867 MS.gene87557:CDS 35.0%
TCTGTACTATTCCAGATTTG+CGG - chr4.1:75306230-75306249 MS.gene87557:CDS 35.0%
TGGAGAATTTCTCAGTCTAT+GGG - chr4.1:75306086-75306105 MS.gene87557:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr4.1:75306085-75306104 MS.gene87557:CDS 35.0%
! ATTTCTCAGTCTATGGGTTA+AGG - chr4.1:75306092-75306111 MS.gene87557:CDS 35.0%
! CAAATCTGGAATAGTACAGA+GGG + chr4.1:75306230-75306249 None:intergenic 35.0%
! TATTTGTCTTTTTGATCCGG+TGG + chr4.1:75305683-75305702 None:intergenic 35.0%
! TGATCAACATCATTTGGAAC+AGG + chr4.1:75306033-75306052 None:intergenic 35.0%
!! CATTAGTACATTCTGGTTTG+TGG - chr4.1:75306058-75306077 MS.gene87557:CDS 35.0%
!! TTGGCTTTGATTTAACGGAA+AGG - chr4.1:75305992-75306011 MS.gene87557:CDS 35.0%
AGAGAGTGAAAGTAGCGATT+CGG + chr4.1:75306400-75306419 None:intergenic 40.0%
CGATTATAGAGAAGGAGAAG+GGG - chr4.1:75305906-75305925 MS.gene87557:CDS 40.0%
GACGATTATAGAGAAGGAGA+AGG - chr4.1:75305904-75305923 MS.gene87557:CDS 40.0%
GAGGGTAATCACTTACCTTA+TGG - chr4.1:75305880-75305899 MS.gene87557:CDS 40.0%
GCTCTGTGATCAACATCATT+TGG + chr4.1:75306039-75306058 None:intergenic 40.0%
GTCTAGAGATGATTATGTGG+TGG - chr4.1:75305762-75305781 MS.gene87557:CDS 40.0%
TACATATGGAATCCATCCAC+CGG - chr4.1:75305664-75305683 MS.gene87557:CDS 40.0%
TATGGCGACGATTATAGAGA+AGG - chr4.1:75305898-75305917 MS.gene87557:CDS 40.0%
TCTCTATAATCGTCGCCATA+AGG + chr4.1:75305898-75305917 None:intergenic 40.0%
TGAGGAACACCAACTAAGAA+TGG + chr4.1:75305811-75305830 None:intergenic 40.0%
TGGTGTTCCTCAATCACATT+TGG - chr4.1:75305819-75305838 MS.gene87557:CDS 40.0%
TTCGGTATACACGACAACTT+CGG + chr4.1:75306382-75306401 None:intergenic 40.0%
! AGAAGGGGTGGTTTTTAATG+GGG - chr4.1:75305921-75305940 MS.gene87557:CDS 40.0%
! AGTCTATGGGTTAAGGATAC+TGG - chr4.1:75306099-75306118 MS.gene87557:CDS 40.0%
! CACAGAGCATTAGTACATTC+TGG - chr4.1:75306051-75306070 MS.gene87557:CDS 40.0%
! GAGAAGGGGTGGTTTTTAAT+GGG - chr4.1:75305920-75305939 MS.gene87557:CDS 40.0%
! GCAAATCTGGAATAGTACAG+AGG + chr4.1:75306231-75306250 None:intergenic 40.0%
!! CATTCTGGTTTGTGGGTATT+TGG - chr4.1:75306066-75306085 MS.gene87557:CDS 40.0%
!! TTCGCGTCGTGATTTAGTTT+TGG - chr4.1:75305960-75305979 MS.gene87557:CDS 40.0%
AGAGGGTTAAAGTAGTGACC+AGG + chr4.1:75306213-75306232 None:intergenic 45.0%
GTATACACGACAACTTCGGA+TGG + chr4.1:75306378-75306397 None:intergenic 45.0%
GTGAAAGTAGTCCGCAAATC+TGG + chr4.1:75306244-75306263 None:intergenic 45.0%
TTATAGAGAAGGAGAAGGGG+TGG - chr4.1:75305909-75305928 MS.gene87557:CDS 45.0%
! GAATCGCTACTTTCACTCTC+TGG - chr4.1:75306399-75306418 MS.gene87557:CDS 45.0%
! GGAGAAGGGGTGGTTTTTAA+TGG - chr4.1:75305919-75305938 MS.gene87557:CDS 45.0%
! TGTCTTTTTGATCCGGTGGA+TGG + chr4.1:75305679-75305698 None:intergenic 45.0%
CTTCCTCAGCATGTCATTCC+TGG - chr4.1:75306192-75306211 MS.gene87557:CDS 50.0%
TGACCAGGAATGACATGCTG+AGG + chr4.1:75306198-75306217 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 75305643 75306440 75305643 ID=MS.gene87557
chr4.1 mRNA 75305643 75306440 75305643 ID=MS.gene87557.t1;Parent=MS.gene87557
chr4.1 exon 75305643 75306440 75305643 ID=MS.gene87557.t1.exon1;Parent=MS.gene87557.t1
chr4.1 CDS 75305643 75306440 75305643 ID=cds.MS.gene87557.t1;Parent=MS.gene87557.t1
Gene Sequence

>MS.gene87557

ATGTATCGTCATCCAAACATATACATATGGAATCCATCCACCGGATCAAAAAGACAAATACTTATGTCTGCTTTTAATTCCGAAGCGTATATTAATCTATATGGGTTTGGTTATGATCAGTCTAGAGATGATTATGTGGTGGTTTTATTGTCCAATAAAGTTAATCCATTCTTAGTTGGTGTTCCTCAATCACATTTGGAGTTTTTCTCGTTTAGAGACAATACTTGGAAAGAAATCGAGGGTAATCACTTACCTTATGGCGACGATTATAGAGAAGGAGAAGGGGTGGTTTTTAATGGGGCTATTCATTGGTTGTCTTCGCGTCGTGATTTAGTTTTGGATGTCATTGTTGGCTTTGATTTAACGGAAAGGAAATTGTTTGAGATGCCTGTTCCAAATGATGTTGATCACAGAGCATTAGTACATTCTGGTTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGGTTAAGGATACTGGTAATGATACAATTGAAATATGGGTTATGAAAGAATACAAAGTGCATTCGTCTTGGACTAAGACTCTTGTTCTTCCTCAGCATGTCATTCCTGGTCACTACTTTAACCCTCTGTACTATTCCAGATTTGCGGACTACTTTCACCCCATGTATTCTACAGAAAATGGTGATATTATTGGAAAATATGGTGGTACTAGATTGGTGAAGTATAATGACAAAGGACAGTTTTTAGGGCATCGCTCTTTCTGCAACAGTCCATCCGAAGTTGTCGTGTATACCGAATCGCTACTTTCACTCTCTGGTGACAATGAGCATGTTTAA
Protein sequence

>MS.gene87557.t1

MYRHPNIYIWNPSTGSKRQILMSAFNSEAYINLYGFGYDQSRDDYVVVLLSNKVNPFLVGVPQSHLEFFSFRDNTWKEIEGNHLPYGDDYREGEGVVFNGAIHWLSSRRDLVLDVIVGFDLTERKLFEMPVPNDVDHRALVHSGLWVFGEFLSLWVKDTGNDTIEIWVMKEYKVHSSWTKTLVLPQHVIPGHYFNPLYYSRFADYFHPMYSTENGDIIGKYGGTRLVKYNDKGQFLGHRSFCNSPSEVVVYTESLLSLSGDNEHV