AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50337


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50337.t1 MTR_4g023090 55.670 194 43 3 1 163 139 320 1.55e-61 194
MS.gene50337.t1 MTR_4g023130 54.688 192 37 4 1 162 90 261 1.10e-60 190
MS.gene50337.t1 MTR_4g023080 53.886 193 39 4 1 163 131 303 2.67e-59 188
MS.gene50337.t1 MTR_4g023440 57.143 161 46 3 3 163 144 281 3.05e-53 172
MS.gene50337.t1 MTR_4g023380 46.047 215 37 3 1 163 139 326 5.24e-52 170
MS.gene50337.t1 MTR_4g021785 50.000 194 46 4 1 163 131 304 2.81e-51 167
MS.gene50337.t1 MTR_4g117720 49.741 193 57 5 1 163 137 319 1.69e-50 166
MS.gene50337.t1 MTR_4g023070 47.120 191 56 3 18 163 129 319 7.58e-49 161
MS.gene50337.t1 MTR_4g023120 46.067 178 63 2 18 162 129 306 7.59e-47 156
MS.gene50337.t1 MTR_6g060650 46.354 192 64 4 1 163 139 320 2.50e-45 152
MS.gene50337.t1 MTR_4g023150 45.918 196 54 5 1 163 132 308 3.16e-43 147
MS.gene50337.t1 MTR_4g122850 47.208 197 59 8 1 163 125 310 5.26e-43 146
MS.gene50337.t1 MTR_3g034180 47.802 182 56 4 20 162 255 436 6.22e-43 149
MS.gene50337.t1 MTR_3g034130 48.352 182 55 4 20 162 141 322 9.14e-43 145
MS.gene50337.t1 MTR_4g118380 44.000 200 64 4 1 162 141 330 1.23e-42 145
MS.gene50337.t1 MTR_8g032390 46.939 196 58 8 1 162 139 322 2.40e-42 144
MS.gene50337.t1 MTR_4g117850 48.588 177 55 5 23 163 150 326 9.03e-42 143
MS.gene50337.t1 MTR_3g034160 46.739 184 57 5 20 163 141 323 4.35e-41 141
MS.gene50337.t1 MTR_3g103020 43.655 197 64 5 1 163 142 325 5.52e-41 141
MS.gene50337.t1 MTR_4g023190 45.918 196 53 6 1 163 132 307 6.24e-41 140
MS.gene50337.t1 MTR_4g094365 43.367 196 62 6 1 162 130 310 6.35e-40 138
MS.gene50337.t1 MTR_4g109280 50.000 160 50 3 33 162 145 304 1.30e-39 137
MS.gene50337.t1 MTR_8g433030 50.000 168 47 7 29 162 125 289 1.66e-39 136
MS.gene50337.t1 MTR_4g116330 42.784 194 64 5 1 163 148 325 2.34e-39 137
MS.gene50337.t1 MTR_4g023200 43.367 196 59 5 1 163 132 308 3.43e-39 136
MS.gene50337.t1 MTR_5g070080 47.904 167 54 4 29 163 168 333 3.90e-39 136
MS.gene50337.t1 MTR_4g116320 47.239 163 52 4 33 163 43 203 2.22e-37 129
MS.gene50337.t1 MTR_8g013010 51.079 139 52 5 1 133 178 306 1.51e-36 128
MS.gene50337.t1 MTR_5g070130 41.243 177 63 4 26 162 147 322 2.77e-36 129
MS.gene50337.t1 MTR_4g118400 51.773 141 49 3 41 162 106 246 4.15e-36 127
MS.gene50337.t1 MTR_4g117770 44.693 179 57 5 20 163 143 314 4.36e-34 123
MS.gene50337.t1 MTR_4g117810 44.693 179 57 5 20 163 143 314 4.36e-34 123
MS.gene50337.t1 MTR_8g040935 44.444 153 50 4 44 162 1 152 6.48e-33 116
MS.gene50337.t1 MTR_5g097210 42.640 197 62 7 1 163 131 310 9.82e-33 119
MS.gene50337.t1 MTR_4g109270 43.077 195 61 7 1 162 133 310 2.91e-32 118
MS.gene50337.t1 MTR_4g116330 45.181 166 61 7 1 163 148 286 3.64e-32 117
MS.gene50337.t1 MTR_5g070088 41.420 169 65 3 29 163 164 332 6.67e-32 117
MS.gene50337.t1 MTR_8g433040 41.837 196 59 6 1 162 138 312 1.21e-31 116
MS.gene50337.t1 MTR_8g032380 41.837 196 59 6 1 162 138 312 1.21e-31 116
MS.gene50337.t1 MTR_4g117780 39.394 198 70 6 1 163 132 314 1.79e-31 116
MS.gene50337.t1 MTR_4g117530 46.324 136 65 2 29 163 75 203 8.36e-31 112
MS.gene50337.t1 MTR_6g463430 43.590 156 47 5 44 164 1 150 1.58e-30 110
MS.gene50337.t1 MTR_4g114700 41.212 165 67 5 1 162 33 170 9.86e-30 108
MS.gene50337.t1 MTR_1g055195 42.197 173 50 6 29 162 263 424 1.41e-29 112
MS.gene50337.t1 MTR_4g117430 44.667 150 62 3 1 150 27 155 3.52e-29 105
MS.gene50337.t1 MTR_4g117750 40.541 185 65 7 21 163 125 306 1.53e-27 107
MS.gene50337.t1 MTR_4g117750 71.795 39 11 0 125 163 392 430 2.19e-11 61.2
MS.gene50337.t1 MTR_4g121580 40.704 199 43 9 1 163 139 298 4.90e-27 104
MS.gene50337.t1 MTR_1g055135 41.279 172 50 5 24 150 145 310 8.80e-27 103
MS.gene50337.t1 MTR_4g118370 37.572 173 55 6 25 163 54 207 7.54e-25 96.7
MS.gene50337.t1 MTR_1g055250 37.931 174 69 6 25 163 46 215 1.00e-24 97.8
MS.gene50337.t1 MTR_6g034720 44.094 127 61 4 1 125 54 172 1.15e-24 95.1
MS.gene50337.t1 MTR_4g117870 39.024 164 80 5 1 163 70 214 3.18e-24 95.1
MS.gene50337.t1 MTR_7g079370 36.747 166 64 5 29 155 133 296 3.08e-22 91.3
MS.gene50337.t1 MTR_4g116340 54.545 88 38 2 25 111 161 247 4.51e-22 89.4
MS.gene50337.t1 MTR_7g117720 39.264 163 39 4 1 162 116 219 7.22e-22 89.4
MS.gene50337.t1 MTR_8g102520 35.088 171 70 6 29 162 101 267 3.66e-20 85.5
MS.gene50337.t1 MTR_1g055175 40.650 123 39 3 57 145 8 130 3.60e-19 80.5
MS.gene50337.t1 MTR_7g079400 32.022 178 84 4 20 162 103 278 4.33e-18 79.7
MS.gene50337.t1 MTR_1g055165 45.977 87 18 1 88 145 161 247 1.18e-15 72.8
MS.gene50337.t1 MTR_8g098830 33.333 144 59 4 54 162 31 172 7.46e-15 69.7
MS.gene50337.t1 MTR_2g048580 33.607 122 45 3 55 140 155 276 2.20e-12 63.9
MS.gene50337.t1 MTR_4g122860 39.286 112 55 5 64 162 158 269 3.67e-11 60.5
MS.gene50337.t1 MTR_5g023000 65.789 38 13 0 125 162 8 45 4.06e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGACAATGTGCGGCTAATT+TGG 0.222275 4.4:-78330656 None:intergenic
AATGAATAGCCCCATTAAAT+AGG 0.222547 4.4:-78330897 None:intergenic
CGCCTTCATATACTTGGTTT+TGG 0.241496 4.4:+78330703 MS.gene50337:CDS
CATTGTTGCCTTTGATTTAA+CGG 0.288851 4.4:+78330954 MS.gene50337:CDS
TATTTAATGGGGCTATTCAT+TGG 0.316240 4.4:+78330899 MS.gene50337:CDS
CTAATGATACAGTTGAAATA+TGG 0.329864 4.4:+78331081 MS.gene50337:intron
ATGGCTTTGAGTCGTGATTT+AGG 0.341932 4.4:+78330922 MS.gene50337:CDS
AGAAAAGGGTCCCTATTTAA+TGG 0.349556 4.4:+78330886 MS.gene50337:CDS
GAAAAGGGTCCCTATTTAAT+GGG 0.364953 4.4:+78330887 MS.gene50337:CDS
CCGGTCAAGAGATGATTATT+TGG 0.374848 4.4:+78330732 MS.gene50337:CDS
GTCACCCATGGTCGCAGAAA+AGG 0.395681 4.4:+78330871 MS.gene50337:CDS
GCCGCACATTGTCATCGTAT+TGG 0.425136 4.4:+78330664 MS.gene50337:CDS
ATCTGCTACCATTGGATCAT+AGG 0.427213 4.4:-78330767 None:intergenic
ATGAATAGCCCCATTAAATA+GGG 0.428993 4.4:-78330896 None:intergenic
TCTTATCGCCTTCATATACT+TGG 0.436134 4.4:+78330697 MS.gene50337:CDS
CTTTGAGTCGTGATTTAGGT+TGG 0.458224 4.4:+78330926 MS.gene50337:CDS
TCACCCATGGTCGCAGAAAA+GGG 0.487287 4.4:+78330872 MS.gene50337:CDS
AATACAAAGTGCATTCGTCT+TGG 0.494278 4.4:+78331114 MS.gene50337:CDS
TAATGGGGCTATTCATTGGA+TGG 0.494477 4.4:+78330903 MS.gene50337:CDS
TAATACATGGAAAGAAATTG+AGG 0.497226 4.4:+78330828 MS.gene50337:CDS
GAAATAGCATCTGCTACCAT+TGG 0.502574 4.4:-78330775 None:intergenic
ATTGGGTGAAAGTATCGATC+AGG 0.504656 4.4:-78331170 None:intergenic
GTCAAGAGATGATTATTTGG+TGG 0.504882 4.4:+78330735 MS.gene50337:CDS
AACCAAAACCAAGTATATGA+AGG 0.524098 4.4:-78330705 None:intergenic
CGATCAGGAATTGCATTAAG+AGG 0.526394 4.4:-78331155 None:intergenic
GCGGCTAATTTGGAGTCAAA+AGG 0.531706 4.4:-78330646 None:intergenic
TTCATTGTCCTATGATCCAA+TGG 0.536743 4.4:+78330759 MS.gene50337:CDS
GTATATGAAGGCGATAAGAC+TGG 0.541415 4.4:-78330693 None:intergenic
TAATGATACAGTTGAAATAT+GGG 0.542942 4.4:+78331082 MS.gene50337:intron
TCTCATTTAGAGATAATACA+TGG 0.548869 4.4:+78330815 MS.gene50337:CDS
CCAAATAATCATCTCTTGAC+CGG 0.557184 4.4:-78330732 None:intergenic
AATACATGGAAAGAAATTGA+GGG 0.574680 4.4:+78330829 MS.gene50337:CDS
TAGTCAAACTCACTTGGAAG+AGG 0.576522 4.4:-78330997 None:intergenic
AGATGCTATTTCAACACATG+TGG 0.578858 4.4:+78330786 MS.gene50337:CDS
TTCCGTTACAATGTCACCCA+TGG 0.611703 4.4:+78330859 MS.gene50337:CDS
GATGCTATTTCAACACATGT+GGG 0.623104 4.4:+78330787 MS.gene50337:CDS
TTGGAGTCAAAAGGAGACAC+GGG 0.631270 4.4:-78330637 None:intergenic
ACCAATACGATGACAATGTG+CGG 0.635287 4.4:-78330665 None:intergenic
ATGCTATTTCAACACATGTG+GGG 0.643893 4.4:+78330788 MS.gene50337:CDS
TTTGGAGTCAAAAGGAGACA+CGG 0.650067 4.4:-78330638 None:intergenic
GACCATGGGTGACATTGTAA+CGG 0.651025 4.4:-78330861 None:intergenic
AAAAGGGTCCCTATTTAATG+GGG 0.672236 4.4:+78330888 MS.gene50337:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGATACAGTTGAAATAT+GGG + chr4.4:78331082-78331101 MS.gene50337:intron 20.0%
!!! ATTTTTTTGTAGAGTACATT+GGG - chr4.4:78331190-78331209 None:intergenic 20.0%
! AATACATGGAAAGAAATTGA+GGG + chr4.4:78330829-78330848 MS.gene50337:CDS 25.0%
! CAATGTACTCTACAAAAAAA+TGG + chr4.4:78331189-78331208 MS.gene50337:CDS 25.0%
! CTAATGATACAGTTGAAATA+TGG + chr4.4:78331081-78331100 MS.gene50337:intron 25.0%
! TAATACATGGAAAGAAATTG+AGG + chr4.4:78330828-78330847 MS.gene50337:CDS 25.0%
! TCTCATTTAGAGATAATACA+TGG + chr4.4:78330815-78330834 MS.gene50337:CDS 25.0%
!!! ATGTAGTTAATTCTAGTTTG+TGG + chr4.4:78331019-78331038 MS.gene50337:intron 25.0%
!!! CATTTTTTTGTAGAGTACAT+TGG - chr4.4:78331191-78331210 None:intergenic 25.0%
!!! TGTAGTTAATTCTAGTTTGT+GGG + chr4.4:78331020-78331039 MS.gene50337:intron 25.0%
AACCAAAACCAAGTATATGA+AGG - chr4.4:78330708-78330727 None:intergenic 30.0%
AATGAATAGCCCCATTAAAT+AGG - chr4.4:78330900-78330919 None:intergenic 30.0%
ATGAATAGCCCCATTAAATA+GGG - chr4.4:78330899-78330918 None:intergenic 30.0%
TATTTAATGGGGCTATTCAT+TGG + chr4.4:78330899-78330918 MS.gene50337:CDS 30.0%
! CATTGTTGCCTTTGATTTAA+CGG + chr4.4:78330954-78330973 MS.gene50337:CDS 30.0%
! TTTTCGTTCCGTTAAATCAA+AGG - chr4.4:78330965-78330984 None:intergenic 30.0%
!! AATTCTAGTTTGTGGGTATA+TGG + chr4.4:78331027-78331046 MS.gene50337:intron 30.0%
AAAAGGGTCCCTATTTAATG+GGG + chr4.4:78330888-78330907 MS.gene50337:CDS 35.0%
AATACAAAGTGCATTCGTCT+TGG + chr4.4:78331114-78331133 MS.gene50337:CDS 35.0%
ACTACATAGTCAAACTCACT+TGG - chr4.4:78331006-78331025 None:intergenic 35.0%
AGAAAAGGGTCCCTATTTAA+TGG + chr4.4:78330886-78330905 MS.gene50337:CDS 35.0%
CCAAATAATCATCTCTTGAC+CGG - chr4.4:78330735-78330754 None:intergenic 35.0%
GAAAAGGGTCCCTATTTAAT+GGG + chr4.4:78330887-78330906 MS.gene50337:CDS 35.0%
GTCAAGAGATGATTATTTGG+TGG + chr4.4:78330735-78330754 MS.gene50337:CDS 35.0%
TCTTATCGCCTTCATATACT+TGG + chr4.4:78330697-78330716 MS.gene50337:CDS 35.0%
TTCATTGTCCTATGATCCAA+TGG + chr4.4:78330759-78330778 MS.gene50337:CDS 35.0%
!! AGATGCTATTTCAACACATG+TGG + chr4.4:78330786-78330805 MS.gene50337:CDS 35.0%
!! ATGCTATTTCAACACATGTG+GGG + chr4.4:78330788-78330807 MS.gene50337:CDS 35.0%
!! GATGCTATTTCAACACATGT+GGG + chr4.4:78330787-78330806 MS.gene50337:CDS 35.0%
!!! TACTTGGTTTTGGTTATGAC+CGG + chr4.4:78330713-78330732 MS.gene50337:CDS 35.0%
AATTTCTCAGTCGATGGCTA+TGG + chr4.4:78331052-78331071 MS.gene50337:intron 40.0%
ACCAATACGATGACAATGTG+CGG - chr4.4:78330668-78330687 None:intergenic 40.0%
ATCTGCTACCATTGGATCAT+AGG - chr4.4:78330770-78330789 None:intergenic 40.0%
ATGACAATGTGCGGCTAATT+TGG - chr4.4:78330659-78330678 None:intergenic 40.0%
ATGGAGAATTTCTCAGTCGA+TGG + chr4.4:78331046-78331065 MS.gene50337:intron 40.0%
CCGGTCAAGAGATGATTATT+TGG + chr4.4:78330732-78330751 MS.gene50337:CDS 40.0%
CGATCAGGAATTGCATTAAG+AGG - chr4.4:78331158-78331177 None:intergenic 40.0%
CTTTGAGTCGTGATTTAGGT+TGG + chr4.4:78330926-78330945 MS.gene50337:CDS 40.0%
GAAATAGCATCTGCTACCAT+TGG - chr4.4:78330778-78330797 None:intergenic 40.0%
GTATATGAAGGCGATAAGAC+TGG - chr4.4:78330696-78330715 None:intergenic 40.0%
TAATGGGGCTATTCATTGGA+TGG + chr4.4:78330903-78330922 MS.gene50337:CDS 40.0%
TAGTCAAACTCACTTGGAAG+AGG - chr4.4:78331000-78331019 None:intergenic 40.0%
! CGCCTTCATATACTTGGTTT+TGG + chr4.4:78330703-78330722 MS.gene50337:CDS 40.0%
! TTTGGAGTCAAAAGGAGACA+CGG - chr4.4:78330641-78330660 None:intergenic 40.0%
!! ATGGCTTTGAGTCGTGATTT+AGG + chr4.4:78330922-78330941 MS.gene50337:CDS 40.0%
!! ATTGGGTGAAAGTATCGATC+AGG - chr4.4:78331173-78331192 None:intergenic 40.0%
GACCATGGGTGACATTGTAA+CGG - chr4.4:78330864-78330883 None:intergenic 45.0%
TTCCGTTACAATGTCACCCA+TGG + chr4.4:78330859-78330878 MS.gene50337:CDS 45.0%
TTGGAGTCAAAAGGAGACAC+GGG - chr4.4:78330640-78330659 None:intergenic 45.0%
! GCGGCTAATTTGGAGTCAAA+AGG - chr4.4:78330649-78330668 None:intergenic 45.0%
GCCGCACATTGTCATCGTAT+TGG + chr4.4:78330664-78330683 MS.gene50337:CDS 50.0%
TCACCCATGGTCGCAGAAAA+GGG + chr4.4:78330872-78330891 MS.gene50337:CDS 50.0%
GTCACCCATGGTCGCAGAAA+AGG + chr4.4:78330871-78330890 MS.gene50337:CDS 55.0%
! GGACCCTTTTCTGCGACCAT+GGG - chr4.4:78330878-78330897 None:intergenic 55.0%
! GGGACCCTTTTCTGCGACCA+TGG - chr4.4:78330879-78330898 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 78330634 78331214 78330634 ID=MS.gene50337
chr4.4 mRNA 78330634 78331214 78330634 ID=MS.gene50337.t1;Parent=MS.gene50337
chr4.4 exon 78330634 78331006 78330634 ID=MS.gene50337.t1.exon1;Parent=MS.gene50337.t1
chr4.4 CDS 78330634 78331006 78330634 ID=cds.MS.gene50337.t1;Parent=MS.gene50337.t1
chr4.4 exon 78331093 78331214 78331093 ID=MS.gene50337.t1.exon2;Parent=MS.gene50337.t1
chr4.4 CDS 78331093 78331214 78331093 ID=cds.MS.gene50337.t1;Parent=MS.gene50337.t1
Gene Sequence

>MS.gene50337

ATGCCCGTGTCTCCTTTTGACTCCAAATTAGCCGCACATTGTCATCGTATTGGTTATGACCAGTCTTATCGCCTTCATATACTTGGTTTTGGTTATGACCGGTCAAGAGATGATTATTTGGTGGTTTCATTGTCCTATGATCCAATGGTAGCAGATGCTATTTCAACACATGTGGGGTTTTTCTCATTTAGAGATAATACATGGAAAGAAATTGAGGGTACTCACTTCCGTTACAATGTCACCCATGGTCGCAGAAAAGGGTCCCTATTTAATGGGGCTATTCATTGGATGGCTTTGAGTCGTGATTTAGGTTGGAGTGTCATTGTTGCCTTTGATTTAACGGAACGAAAATTGTTTGAGATGCCTCTTCCAAGTGAGTTTGACTATGTAGTTAATTCTAGTTTGTGGGTATATGGAGAATTTCTCAGTCGATGGCTATGGATTATGCTAATGATACAGTTGAAATATGGGTGATGAAAGAATACAAAGTGCATTCGTCTTGGACTAAAACTCTTGTTCTTCCTCTTAATGCAATTCCTGATCGATACTTTCACCCAATGTACTCTACAAAAAAATGGTGA

Protein sequence

>MS.gene50337.t1

MPVSPFDSKLAAHCHRIGYDQSYRLHILGFGYDRSRDDYLVVSLSYDPMVADAISTHVGFFSFRDNTWKEIEGTHFRYNVTHGRRKGSLFNGAIHWMALSRDLGWSVIVAFDLTERKLFEMPLPIEIWVMKEYKVHSSWTKTLVLPLNAIPDRYFHPMYSTKKW