AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062698


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062698.t1 MTR_1g009110 72.348 264 67 3 31 290 118 379 4.59e-130 374
MS.gene062698.t1 MTR_1g022105 69.198 237 28 4 31 267 127 318 7.02e-105 308
MS.gene062698.t1 MTR_1g023400 42.435 271 134 9 29 289 94 352 1.03e-56 186
MS.gene062698.t1 MTR_1g022200 40.468 299 154 9 9 290 79 370 1.28e-55 184
MS.gene062698.t1 MTR_4g080200 41.852 270 141 7 29 289 87 349 3.66e-55 182
MS.gene062698.t1 MTR_1g037540 41.065 263 125 14 49 289 146 400 5.24e-42 149
MS.gene062698.t1 MTR_1g023430 47.368 133 69 1 158 290 42 173 1.11e-33 121
MS.gene062698.t1 MTR_1g024125 51.923 104 46 2 161 260 38 141 4.08e-29 108
MS.gene062698.t1 MTR_1g008000 42.553 141 66 8 159 290 135 269 4.66e-15 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 60 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAGTATGATAAATGTTTA+TGG 0.150614 1.4:+2334763 MS.gene062698:CDS
GTCCTGTCCGGTTAGAAATT+TGG 0.176280 1.4:+2334565 MS.gene062698:CDS
AAATATGATGATTCCTTATA+TGG 0.185379 1.4:+2334168 MS.gene062698:CDS
TTGTTAACTACGTTTATTAT+TGG 0.195963 1.4:+2334376 MS.gene062698:CDS
CACATGGCAATTGCAATTAA+TGG 0.267597 1.4:+2334027 MS.gene062698:CDS
GGGTTCACTTGCTTATGTTT+TGG 0.277483 1.4:+2334527 MS.gene062698:CDS
ATGAGGGTGCAGGAGGGATA+TGG 0.328189 1.4:-2334134 None:intergenic
AAAGTATGATAAATGTTTAT+GGG 0.339753 1.4:+2334764 MS.gene062698:CDS
TATTGGAATTCAACAAATCT+TGG 0.344030 1.4:+2334072 MS.gene062698:CDS
CCGGTTAGAAATTTGGATTA+TGG 0.356395 1.4:+2334572 MS.gene062698:CDS
GAAGGAGGAGGGTGATGGAC+AGG 0.358181 1.4:-2334312 None:intergenic
AAGCCGATGCTTTATGCTTC+GGG 0.360072 1.4:+2334252 MS.gene062698:CDS
GAAGCCGATGCTTTATGCTT+CGG 0.376318 1.4:+2334251 MS.gene062698:CDS
AAAGGCACAGAAACCATATA+AGG 0.408235 1.4:-2334181 None:intergenic
AACTTTGAAATTATTTCTAA+AGG 0.414378 1.4:-2334199 None:intergenic
TGAAAACATCGTCGAGATCA+AGG 0.419509 1.4:+2334506 MS.gene062698:CDS
ATGTGATTCAATGTTGTGTA+TGG 0.428693 1.4:-2334009 None:intergenic
ATAACTGTGATCCGGTTAGA+AGG 0.432134 1.4:-2334330 None:intergenic
ATCACCCTCCTCCTTCTAAC+CGG 0.446237 1.4:+2334319 MS.gene062698:CDS
CCATAATCCAAATTTCTAAC+CGG 0.447165 1.4:-2334572 None:intergenic
TAATAAACGTAGTTAACAAC+TGG 0.454507 1.4:-2334372 None:intergenic
TTTATTATTAGCAGGAAAGA+GGG 0.459387 1.4:-2333985 None:intergenic
CTACAACACGTCCACTCAAC+CGG 0.459439 1.4:-2334630 None:intergenic
AAGTTTGAGATTTGCTATGC+AGG 0.475011 1.4:-2334734 None:intergenic
TTCAACATGAGGTTGTCGTT+GGG 0.478589 1.4:+2334229 MS.gene062698:CDS
CTTCAACATGAGGTTGTCGT+TGG 0.499378 1.4:+2334228 MS.gene062698:CDS
ATTTCTAACCGGACAGGACT+AGG 0.503309 1.4:-2334561 None:intergenic
ATCATCTTCCTAGTCCTGTC+CGG 0.513742 1.4:+2334553 MS.gene062698:CDS
GCTAGCTAGCTAGGTTGACA+AGG 0.514125 1.4:-2334814 None:intergenic
CTGACTTCGTATGAAGATCA+AGG 0.526393 1.4:+2334708 MS.gene062698:CDS
GATTATAAATAACTGTGATC+CGG 0.527165 1.4:-2334338 None:intergenic
CGGCGCCGACGATGAGGAGG+TGG 0.528618 1.4:+2333944 None:intergenic
ATCTTGGTCACTTGATTCTG+TGG 0.533610 1.4:+2334088 MS.gene062698:CDS
AAGGAGGAGGGTGATGGACA+GGG 0.542016 1.4:-2334311 None:intergenic
ATCCAAATTTCTAACCGGAC+AGG 0.542204 1.4:-2334567 None:intergenic
GGTTAGAAGGAGGAGGGTGA+TGG 0.546100 1.4:-2334317 None:intergenic
TTAACAACTGGTGTATAAGC+AGG 0.547126 1.4:-2334360 None:intergenic
TCAAAGAATGAGGGTGCAGG+AGG 0.547678 1.4:-2334141 None:intergenic
GAGTCAAAGAATGAGGGTGC+AGG 0.549550 1.4:-2334144 None:intergenic
GTGATCCGGTTAGAAGGAGG+AGG 0.550362 1.4:-2334324 None:intergenic
TTTGGATTATGGATCAAAGT+GGG 0.554416 1.4:+2334583 MS.gene062698:CDS
ATAACATTGGTCCGGTTGAG+TGG 0.557869 1.4:+2334619 MS.gene062698:CDS
GAAAACATCGTCGAGATCAA+GGG 0.557945 1.4:+2334507 MS.gene062698:CDS
ATATTTAGAGTCAAAGAATG+AGG 0.562684 1.4:-2334151 None:intergenic
ACGATCTAAGCACTCAGTCA+TGG 0.566417 1.4:+2334286 MS.gene062698:CDS
AAGTATGATAAATGTTTATG+GGG 0.574545 1.4:+2334765 MS.gene062698:CDS
ATTTGGATTATGGATCAAAG+TGG 0.575328 1.4:+2334582 MS.gene062698:CDS
TGACGGCGCCGACGATGAGG+AGG 0.608116 1.4:+2333941 None:intergenic
GATTATGGATCAAAGTGGGT+GGG 0.612527 1.4:+2334587 MS.gene062698:CDS
AGGAGGAGGGTGATGGACAG+GGG 0.614131 1.4:-2334310 None:intergenic
GGATTATGGATCAAAGTGGG+TGG 0.615476 1.4:+2334586 MS.gene062698:CDS
CTAGCTAGCTAGGTTGACAA+GGG 0.623600 1.4:-2334813 None:intergenic
GCTCCCGAAGCATAAAGCAT+CGG 0.629012 1.4:-2334255 None:intergenic
TATTTAGAGTCAAAGAATGA+GGG 0.639125 1.4:-2334150 None:intergenic
AGTTGTTAGACTTCAACATG+AGG 0.650334 1.4:+2334218 MS.gene062698:CDS
ACTGTGATCCGGTTAGAAGG+AGG 0.659902 1.4:-2334327 None:intergenic
ATACACAACATTGAATCACA+TGG 0.666706 1.4:+2334011 MS.gene062698:CDS
TGATCCGGTTAGAAGGAGGA+GGG 0.681484 1.4:-2334323 None:intergenic
CAAAGAATGAGGGTGCAGGA+GGG 0.683552 1.4:-2334140 None:intergenic
TAATGTTCAACAGCAATACG+CGG 0.694168 1.4:-2334480 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTATGATAAATGTTTAT+GGG + chr1.4:2334764-2334783 MS.gene062698:CDS 15.0%
!! TAAAGTATGATAAATGTTTA+TGG + chr1.4:2334763-2334782 MS.gene062698:CDS 15.0%
!!! AACTTTGAAATTATTTCTAA+AGG - chr1.4:2334202-2334221 None:intergenic 15.0%
!!! TCTTTTTAAAAGCTTTTTAT+TGG + chr1.4:2334055-2334074 MS.gene062698:CDS 15.0%
!! AAATATGATGATTCCTTATA+TGG + chr1.4:2334168-2334187 MS.gene062698:CDS 20.0%
!! AAGTATGATAAATGTTTATG+GGG + chr1.4:2334765-2334784 MS.gene062698:CDS 20.0%
!! TTGTTAACTACGTTTATTAT+TGG + chr1.4:2334376-2334395 MS.gene062698:CDS 20.0%
! GATTATAAATAACTGTGATC+CGG - chr1.4:2334341-2334360 None:intergenic 25.0%
! TAATAAACGTAGTTAACAAC+TGG - chr1.4:2334375-2334394 None:intergenic 25.0%
! TATTGGAATTCAACAAATCT+TGG + chr1.4:2334072-2334091 MS.gene062698:CDS 25.0%
! TTTATTATTAGCAGGAAAGA+GGG - chr1.4:2333988-2334007 None:intergenic 25.0%
!! ATATTTAGAGTCAAAGAATG+AGG - chr1.4:2334154-2334173 None:intergenic 25.0%
!! TATTTAGAGTCAAAGAATGA+GGG - chr1.4:2334153-2334172 None:intergenic 25.0%
!! TTTTATTATTAGCAGGAAAG+AGG - chr1.4:2333989-2334008 None:intergenic 25.0%
!!! TGTATGGTTTTATTATTAGC+AGG - chr1.4:2333996-2334015 None:intergenic 25.0%
ATACACAACATTGAATCACA+TGG + chr1.4:2334011-2334030 MS.gene062698:CDS 30.0%
ATGTGATTCAATGTTGTGTA+TGG - chr1.4:2334012-2334031 None:intergenic 30.0%
ATTTGGATTATGGATCAAAG+TGG + chr1.4:2334582-2334601 MS.gene062698:CDS 30.0%
CCATAATCCAAATTTCTAAC+CGG - chr1.4:2334575-2334594 None:intergenic 30.0%
TTTGGATTATGGATCAAAGT+GGG + chr1.4:2334583-2334602 MS.gene062698:CDS 30.0%
! GAAAAAGTATAACATTGGTC+CGG + chr1.4:2334611-2334630 MS.gene062698:CDS 30.0%
! TGGGTGAAAAAGTATAACAT+TGG + chr1.4:2334606-2334625 MS.gene062698:CDS 30.0%
AGTTGTTAGACTTCAACATG+AGG + chr1.4:2334218-2334237 MS.gene062698:CDS 35.0%
CACATGGCAATTGCAATTAA+TGG + chr1.4:2334027-2334046 MS.gene062698:CDS 35.0%
CCGGTTAGAAATTTGGATTA+TGG + chr1.4:2334572-2334591 MS.gene062698:CDS 35.0%
TAATGTTCAACAGCAATACG+CGG - chr1.4:2334483-2334502 None:intergenic 35.0%
TTAACAACTGGTGTATAAGC+AGG - chr1.4:2334363-2334382 None:intergenic 35.0%
! AAAGGCACAGAAACCATATA+AGG - chr1.4:2334184-2334203 None:intergenic 35.0%
!! AAGTTTGAGATTTGCTATGC+AGG - chr1.4:2334737-2334756 None:intergenic 35.0%
!! TTGTAGACGTAGTTTTTGGA+AGG + chr1.4:2334647-2334666 MS.gene062698:CDS 35.0%
ATAACTGTGATCCGGTTAGA+AGG - chr1.4:2334333-2334352 None:intergenic 40.0%
ATCCAAATTTCTAACCGGAC+AGG - chr1.4:2334570-2334589 None:intergenic 40.0%
ATCTTGGTCACTTGATTCTG+TGG + chr1.4:2334088-2334107 MS.gene062698:CDS 40.0%
CTGACTTCGTATGAAGATCA+AGG + chr1.4:2334708-2334727 MS.gene062698:CDS 40.0%
GAAAACATCGTCGAGATCAA+GGG + chr1.4:2334507-2334526 MS.gene062698:CDS 40.0%
GATTATGGATCAAAGTGGGT+GGG + chr1.4:2334587-2334606 MS.gene062698:CDS 40.0%
TGAAAACATCGTCGAGATCA+AGG + chr1.4:2334506-2334525 MS.gene062698:CDS 40.0%
TTCAACATGAGGTTGTCGTT+GGG + chr1.4:2334229-2334248 MS.gene062698:CDS 40.0%
! CGTGTTGTAGACGTAGTTTT+TGG + chr1.4:2334643-2334662 MS.gene062698:CDS 40.0%
! GGGTTCACTTGCTTATGTTT+TGG + chr1.4:2334527-2334546 MS.gene062698:CDS 40.0%
!! AGACGTAGTTTTTGGAAGGA+TGG + chr1.4:2334651-2334670 MS.gene062698:CDS 40.0%
AAGCCGATGCTTTATGCTTC+GGG + chr1.4:2334252-2334271 MS.gene062698:CDS 45.0%
ACGATCTAAGCACTCAGTCA+TGG + chr1.4:2334286-2334305 MS.gene062698:CDS 45.0%
ATAACATTGGTCCGGTTGAG+TGG + chr1.4:2334619-2334638 MS.gene062698:CDS 45.0%
ATCATCTTCCTAGTCCTGTC+CGG + chr1.4:2334553-2334572 MS.gene062698:CDS 45.0%
ATTTCTAACCGGACAGGACT+AGG - chr1.4:2334564-2334583 None:intergenic 45.0%
CTTCAACATGAGGTTGTCGT+TGG + chr1.4:2334228-2334247 MS.gene062698:CDS 45.0%
GAAGCCGATGCTTTATGCTT+CGG + chr1.4:2334251-2334270 MS.gene062698:CDS 45.0%
GGATTATGGATCAAAGTGGG+TGG + chr1.4:2334586-2334605 MS.gene062698:CDS 45.0%
GTCCTGTCCGGTTAGAAATT+TGG + chr1.4:2334565-2334584 MS.gene062698:CDS 45.0%
! AAGGATGGCGATCGAGTTTT+TGG + chr1.4:2334666-2334685 MS.gene062698:CDS 45.0%
!! CGAGTTTTTGGAGGAAAGGT+TGG + chr1.4:2334678-2334697 MS.gene062698:CDS 45.0%
!! CGATCGAGTTTTTGGAGGAA+AGG + chr1.4:2334674-2334693 MS.gene062698:CDS 45.0%
ACTGTGATCCGGTTAGAAGG+AGG - chr1.4:2334330-2334349 None:intergenic 50.0%
ATCACCCTCCTCCTTCTAAC+CGG + chr1.4:2334319-2334338 MS.gene062698:CDS 50.0%
CTACAACACGTCCACTCAAC+CGG - chr1.4:2334633-2334652 None:intergenic 50.0%
GCTCCCGAAGCATAAAGCAT+CGG - chr1.4:2334258-2334277 None:intergenic 50.0%
TGATCCGGTTAGAAGGAGGA+GGG - chr1.4:2334326-2334345 None:intergenic 50.0%
! CAAAGAATGAGGGTGCAGGA+GGG - chr1.4:2334143-2334162 None:intergenic 50.0%
! GAGTCAAAGAATGAGGGTGC+AGG - chr1.4:2334147-2334166 None:intergenic 50.0%
! TCAAAGAATGAGGGTGCAGG+AGG - chr1.4:2334144-2334163 None:intergenic 50.0%
!! GATGGCGATCGAGTTTTTGG+AGG + chr1.4:2334669-2334688 MS.gene062698:CDS 50.0%
GGTTAGAAGGAGGAGGGTGA+TGG - chr1.4:2334320-2334339 None:intergenic 55.0%
GTGATCCGGTTAGAAGGAGG+AGG - chr1.4:2334327-2334346 None:intergenic 55.0%
! ATGAGGGTGCAGGAGGGATA+TGG - chr1.4:2334137-2334156 None:intergenic 55.0%
!! AAGGAGGAGGGTGATGGACA+GGG - chr1.4:2334314-2334333 None:intergenic 55.0%
!! AGGAGGAGGGTGATGGACAG+GGG - chr1.4:2334313-2334332 None:intergenic 60.0%
!! GAAGGAGGAGGGTGATGGAC+AGG - chr1.4:2334315-2334334 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 2333955 2334827 2333955 ID=MS.gene062698
chr1.4 mRNA 2333955 2334827 2333955 ID=MS.gene062698.t1;Parent=MS.gene062698
chr1.4 exon 2333955 2334827 2333955 ID=MS.gene062698.t1.exon1;Parent=MS.gene062698.t1
chr1.4 CDS 2333955 2334827 2333955 ID=cds.MS.gene062698.t1;Parent=MS.gene062698.t1
Gene Sequence

>MS.gene062698

ATGAGGAGGTGGAGCGCATCTGCTTCTACTCCCTCTTTCCTGCTAATAATAAAACCATACACAACATTGAATCACATGGCAATTGCAATTAATGGTGTTTTCTTTTTAAAAGCTTTTTATTGGAATTCAACAAATCTTGGTCACTTGATTCTGTGGAACACAACAGTTAAACAAGTTCACCATATCCCTCCTGCACCCTCATTCTTTGACTCTAAATATGATGATTCCTTATATGGTTTCTGTGCCTTTAGAAATAATTTCAAAGTTGTTAGACTTCAACATGAGGTTGTCGTTGGGAAGCCGATGCTTTATGCTTCGGGAGCAGAAGTTTACGATCTAAGCACTCAGTCATGGACCCCTGTCCATCACCCTCCTCCTTCTAACCGGATCACAGTTATTTATAATCCTGCTTATACACCAGTTGTTAACTACGTTTATTATTGGATTACGACTGTCAATTTTTCTACTATTTCAAACATTCTTTGTTTCGACTTTTGCAACATTCAGTTTTACGAACTCAAAGCTCCGCGTATTGCTGTTGAACATTATATTGAAAACATCGTCGAGATCAAGGGTTCACTTGCTTATGTTTTGGAGTATCATCTTCCTAGTCCTGTCCGGTTAGAAATTTGGATTATGGATCAAAGTGGGTGGGTGAAAAAGTATAACATTGGTCCGGTTGAGTGGACGTGTTGTAGACGTAGTTTTTGGAAGGATGGCGATCGAGTTTTTGGAGGAAAGGTTGGACAACTGCTGACTTCGTATGAAGATCAAGGAAACCTGCATAGCAAATCTCAAACTTATCTATTAAAGTATGATAAATGTTTATGGGGTCATGAGTATTTGCAGAGCATAACTCCCTTGTCAACCTAG

Protein sequence

>MS.gene062698.t1

MRRWSASASTPSFLLIIKPYTTLNHMAIAINGVFFLKAFYWNSTNLGHLILWNTTVKQVHHIPPAPSFFDSKYDDSLYGFCAFRNNFKVVRLQHEVVVGKPMLYASGAEVYDLSTQSWTPVHHPPPSNRITVIYNPAYTPVVNYVYYWITTVNFSTISNILCFDFCNIQFYELKAPRIAVEHYIENIVEIKGSLAYVLEYHLPSPVRLEIWIMDQSGWVKKYNIGPVEWTCCRRSFWKDGDRVFGGKVGQLLTSYEDQGNLHSKSQTYLLKYDKCLWGHEYLQSITPLST