AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86273

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86273.t1 MTR_1g009110 94.603 315 14 1 1 315 68 379 0.0 623
MS.gene86273.t1 MTR_1g022105 57.770 296 73 7 1 294 75 320 5.10e-104 307
MS.gene86273.t1 MTR_1g023400 45.513 312 140 11 13 314 61 352 8.09e-75 234
MS.gene86273.t1 MTR_4g080200 43.689 309 155 11 13 314 53 349 9.06e-66 210
MS.gene86273.t1 MTR_1g022200 44.969 318 144 13 11 315 71 370 1.20e-64 208
MS.gene86273.t1 MTR_1g037540 41.892 296 147 12 36 314 113 400 6.67e-57 189
MS.gene86273.t1 MTR_1g023430 51.493 134 64 1 182 315 41 173 6.47e-39 135
MS.gene86273.t1 MTR_1g024125 52.727 110 48 2 186 291 38 147 1.09e-31 116
MS.gene86273.t1 MTR_1g008000 33.333 285 120 17 47 315 39 269 3.68e-19 85.9
MS.gene86273.t1 MTR_8g046310 28.111 217 128 11 55 258 118 319 6.33e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 92 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTACTATAGATGTTTA+TGG 0.171813 1.3:-10624997 MS.gene86273:CDS
TGGCCCAATGTTGTACATTT+TGG 0.236356 1.3:+10625150 None:intergenic
GGGCTCCCTTGCTTATGTTT+TGG 0.242082 1.3:-10625233 MS.gene86273:CDS
CATGTTGCATAAGTGATTTC+TGG 0.246825 1.3:-10625117 MS.gene86273:CDS
TACTTCTGCTCCCTCTTTCC+TGG 0.291167 1.3:-10625784 MS.gene86273:CDS
CGAACTGGGATGCGGTTAAA+TGG 0.329209 1.3:+10625430 None:intergenic
GAATTACTATAGATGTTTAT+GGG 0.331663 1.3:-10624996 MS.gene86273:CDS
GTCCGATCCAGTTGGAAATC+TGG 0.346576 1.3:-10625195 MS.gene86273:CDS
AAATATGATGATTCCCTGTA+TGG 0.359503 1.3:-10625601 MS.gene86273:CDS
GGTGGACCTGTTTAGTTGTT+GGG 0.363221 1.3:+10625656 None:intergenic
AAGGATGGCGATCAAGTCTT+TGG 0.371759 1.3:-10625094 MS.gene86273:CDS
ATTTATCACAGACCCATTGA+TGG 0.376040 1.3:-10625877 MS.gene86273:CDS
TTGTTAACTGTGTTTATCAT+TGG 0.385806 1.3:-10625384 MS.gene86273:CDS
CCAGTTGGAAATCTGGATTA+TGG 0.392026 1.3:-10625188 MS.gene86273:CDS
CTCTTGGTCAATTGATCCTA+TGG 0.400602 1.3:-10625681 MS.gene86273:CDS
ATATGTTTCGATTCGGTCTA+CGG 0.400923 1.3:+10625753 None:intergenic
ATGCAACATGTCCACGGAAC+TGG 0.401019 1.3:+10625130 None:intergenic
AATGGTGGAGGGTGATGGAC+AGG 0.404814 1.3:+10625448 None:intergenic
CGGTCTACGGATTGATTACC+AGG 0.408180 1.3:+10625766 None:intergenic
AGGTGGACCTGTTTAGTTGT+TGG 0.412563 1.3:+10625655 None:intergenic
TATGATGGATAGTATCGAAC+TGG 0.414566 1.3:+10625415 None:intergenic
GAGAAGATGATTATTCCTTC+AGG 0.423139 1.3:-10625508 MS.gene86273:CDS
TGATAAACACAGTTAACAAC+CGG 0.423981 1.3:+10625388 None:intergenic
CTCTCCTGTGACGGTGGCGA+CGG 0.438612 1.3:-10625829 MS.gene86273:CDS
CAAGTCTTTGGAGGAAAGGT+TGG 0.443153 1.3:-10625082 MS.gene86273:CDS
GTTTAGTTGTTGGGTTCCAT+AGG 0.448822 1.3:+10625665 None:intergenic
ATGGAACCCAACAACTAAAC+AGG 0.452752 1.3:-10625662 MS.gene86273:CDS
CGAAAACATTGCTGAGATCA+AGG 0.453867 1.3:-10625254 MS.gene86273:CDS
AATTGCCATATGTTTCGATT+CGG 0.459622 1.3:+10625746 None:intergenic
GCCTCCGTCGCCACCGTCAC+AGG 0.460383 1.3:+10625825 None:intergenic
ACAGGAGAGAAGAGTGATGT+AGG 0.461686 1.3:+10625843 None:intergenic
AGAATGTTTAACGTGCCGAC+AGG 0.462860 1.3:+10625279 None:intergenic
GGACCAAAATGTACAACATT+GGG 0.464466 1.3:-10625153 MS.gene86273:CDS
ATTTCCAACTGGATCGGACT+AGG 0.486938 1.3:+10625199 None:intergenic
TCCTGTGACGGTGGCGACGG+AGG 0.500634 1.3:-10625826 MS.gene86273:CDS
GGGAGGTTTGAATCCATCAA+TGG 0.500733 1.3:+10625864 None:intergenic
TGGCGACGGAGGCGAGGAGT+TGG 0.501551 1.3:-10625815 MS.gene86273:CDS
CCATAATCCAGATTTCCAAC+TGG 0.510407 1.3:+10625188 None:intergenic
ATGATCTAAGCACTAAATCA+TGG 0.514952 1.3:-10625474 MS.gene86273:CDS
GAGTGACAGAATGAGGGTGC+AGG 0.515232 1.3:+10625625 None:intergenic
AGGAAAGGTTGGAAAGCTGC+TGG 0.518994 1.3:-10625071 MS.gene86273:CDS
CAGGAGAGAAGAGTGATGTA+GGG 0.521709 1.3:+10625844 None:intergenic
GTAGACCGAATCGAAACATA+TGG 0.532232 1.3:-10625751 MS.gene86273:CDS
CGGATTGATTACCAGGAAAG+AGG 0.534555 1.3:+10625773 None:intergenic
CGATCAAGTCTTTGGAGGAA+AGG 0.536754 1.3:-10625086 MS.gene86273:CDS
TCTGGATTATGGATCAGAAC+CGG 0.536974 1.3:-10625177 MS.gene86273:CDS
TAAATGCACAGAAACCATAC+AGG 0.538550 1.3:+10625587 None:intergenic
ATATTTAGAGTGACAGAATG+AGG 0.539503 1.3:+10625618 None:intergenic
TGGACCAAAATGTACAACAT+TGG 0.539844 1.3:-10625154 MS.gene86273:CDS
CTGGCTTCATATGACGATCA+AGG 0.540639 1.3:-10625052 MS.gene86273:CDS
GGTTAAATGGTGGAGGGTGA+TGG 0.542091 1.3:+10625443 None:intergenic
TGAGGTGAGCTAGGTTGACA+AGG 0.543773 1.3:+10624946 None:intergenic
TTAACAACCGGGGTATATGA+TGG 0.545027 1.3:+10625400 None:intergenic
TGACGGTGGCGACGGAGGCG+AGG 0.546343 1.3:-10625821 MS.gene86273:CDS
GGAGGTTTGAATCCATCAAT+GGG 0.551734 1.3:+10625865 None:intergenic
ACTCTTCTCTCCTGTGACGG+TGG 0.556337 1.3:-10625835 MS.gene86273:CDS
AATTACTATAGATGTTTATG+GGG 0.557410 1.3:-10624995 MS.gene86273:CDS
GAGAGAAGAGTGATGTAGGG+AGG 0.559362 1.3:+10625847 None:intergenic
ATCACTCTTCTCTCCTGTGA+CGG 0.564386 1.3:-10625838 MS.gene86273:CDS
GATGGCGATCAAGTCTTTGG+AGG 0.568823 1.3:-10625091 MS.gene86273:CDS
GAAAACATTGCTGAGATCAA+GGG 0.570059 1.3:-10625253 MS.gene86273:CDS
ATCCAGATTTCCAACTGGAT+CGG 0.573766 1.3:+10625193 None:intergenic
GATACTCCAAAACATAAGCA+AGG 0.575880 1.3:+10625227 None:intergenic
AGTGACAGAATGAGGGTGCA+GGG 0.576394 1.3:+10625626 None:intergenic
ATAAGTGATTTCTGGAAGGA+TGG 0.580005 1.3:-10625109 MS.gene86273:CDS
GCCGACAGGGAGCTTTGAGT+TGG 0.583956 1.3:+10625293 None:intergenic
GAAACATATGGCAATTGCAA+TGG 0.588891 1.3:-10625739 MS.gene86273:CDS
GATAGTATCGAACTGGGATG+CGG 0.590112 1.3:+10625422 None:intergenic
TCATACACTTCTGCGCCTGA+AGG 0.591235 1.3:+10625493 None:intergenic
GGGATGCGGTTAAATGGTGG+AGG 0.595710 1.3:+10625436 None:intergenic
GGATTGATTACCAGGAAAGA+GGG 0.601607 1.3:+10625774 None:intergenic
ATGGTGGAGGGTGATGGACA+GGG 0.604225 1.3:+10625449 None:intergenic
TCATCCTAGTCCGATCCAGT+TGG 0.605888 1.3:-10625203 MS.gene86273:CDS
ACAACATTGGGCCAGTTCCG+TGG 0.611189 1.3:-10625141 MS.gene86273:CDS
ACTGGGATGCGGTTAAATGG+TGG 0.612497 1.3:+10625433 None:intergenic
TGGTGGAGGGTGATGGACAG+GGG 0.617953 1.3:+10625450 None:intergenic
TCACTTATGCAACATGTCCA+CGG 0.629494 1.3:+10625124 None:intergenic
GAATGTTTAACGTGCCGACA+GGG 0.630721 1.3:+10625280 None:intergenic
TATTTAGAGTGACAGAATGA+GGG 0.632064 1.3:+10625619 None:intergenic
GAGGTGAGCTAGGTTGACAA+GGG 0.632872 1.3:+10624947 None:intergenic
TGACAGAATGAGGGTGCAGG+GGG 0.633633 1.3:+10625628 None:intergenic
ATACTCCAAAACATAAGCAA+GGG 0.638978 1.3:+10625228 None:intergenic
GTGACAGAATGAGGGTGCAG+GGG 0.639675 1.3:+10625627 None:intergenic
GATAAACACAGTTAACAACC+GGG 0.639807 1.3:+10625389 None:intergenic
GGATTATGGATCAGAACCGG+TGG 0.658826 1.3:-10625174 MS.gene86273:CDS
ATGATGGATAGTATCGAACT+GGG 0.666209 1.3:+10625416 None:intergenic
TTGCATAAGTGATTTCTGGA+AGG 0.673241 1.3:-10625113 MS.gene86273:CDS
ATACTATCCATCATATACCC+CGG 0.677642 1.3:-10625407 MS.gene86273:CDS
GGATGCGGTTAAATGGTGGA+GGG 0.686361 1.3:+10625437 None:intergenic
ACCAACTCAAAGCTCCCTGT+CGG 0.686762 1.3:-10625294 MS.gene86273:CDS
AAATGCACAGAAACCATACA+GGG 0.706345 1.3:+10625588 None:intergenic
ATAAACACAGTTAACAACCG+GGG 0.720377 1.3:+10625390 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTACTATAGATGTTTATG+GGG - chr1.3:10625840-10625859 MS.gene86273:CDS 20.0%
!! GAATTACTATAGATGTTTAT+GGG - chr1.3:10625839-10625858 MS.gene86273:CDS 20.0%
!! TGAATTACTATAGATGTTTA+TGG - chr1.3:10625838-10625857 MS.gene86273:CDS 20.0%
!!! TCTTCTTAAAAGCTTTTTAT+AGG - chr1.3:10625124-10625143 MS.gene86273:CDS 20.0%
! TTGTTAACTGTGTTTATCAT+TGG - chr1.3:10625451-10625470 MS.gene86273:CDS 25.0%
!! TTTTATAGGAATTCAACTCT+TGG - chr1.3:10625138-10625157 MS.gene86273:CDS 25.0%
AAATATGATGATTCCCTGTA+TGG - chr1.3:10625234-10625253 MS.gene86273:CDS 30.0%
AATTGCCATATGTTTCGATT+CGG + chr1.3:10625092-10625111 None:intergenic 30.0%
ATACTCCAAAACATAAGCAA+GGG + chr1.3:10625610-10625629 None:intergenic 30.0%
ATGATCTAAGCACTAAATCA+TGG - chr1.3:10625361-10625380 MS.gene86273:CDS 30.0%
TGATAAACACAGTTAACAAC+CGG + chr1.3:10625450-10625469 None:intergenic 30.0%
! ATATTTAGAGTGACAGAATG+AGG + chr1.3:10625220-10625239 None:intergenic 30.0%
! TATTTAGAGTGACAGAATGA+GGG + chr1.3:10625219-10625238 None:intergenic 30.0%
AAATGCACAGAAACCATACA+GGG + chr1.3:10625250-10625269 None:intergenic 35.0%
ATAAACACAGTTAACAACCG+GGG + chr1.3:10625448-10625467 None:intergenic 35.0%
ATACTATCCATCATATACCC+CGG - chr1.3:10625428-10625447 MS.gene86273:CDS 35.0%
ATTTATCACAGACCCATTGA+TGG - chr1.3:10624958-10624977 MS.gene86273:CDS 35.0%
CATGTTGCATAAGTGATTTC+TGG - chr1.3:10625718-10625737 MS.gene86273:CDS 35.0%
GAAAACATTGCTGAGATCAA+GGG - chr1.3:10625582-10625601 MS.gene86273:CDS 35.0%
GAAACATATGGCAATTGCAA+TGG - chr1.3:10625096-10625115 MS.gene86273:CDS 35.0%
GAGAAGATGATTATTCCTTC+AGG - chr1.3:10625327-10625346 MS.gene86273:CDS 35.0%
GATAAACACAGTTAACAACC+GGG + chr1.3:10625449-10625468 None:intergenic 35.0%
GATACTCCAAAACATAAGCA+AGG + chr1.3:10625611-10625630 None:intergenic 35.0%
GGACCAAAATGTACAACATT+GGG - chr1.3:10625682-10625701 MS.gene86273:CDS 35.0%
TAAATGCACAGAAACCATAC+AGG + chr1.3:10625251-10625270 None:intergenic 35.0%
TGGACCAAAATGTACAACAT+TGG - chr1.3:10625681-10625700 MS.gene86273:CDS 35.0%
! ATAAGTGATTTCTGGAAGGA+TGG - chr1.3:10625726-10625745 MS.gene86273:CDS 35.0%
! TTGCATAAGTGATTTCTGGA+AGG - chr1.3:10625722-10625741 MS.gene86273:CDS 35.0%
!! ATATGTTTCGATTCGGTCTA+CGG + chr1.3:10625085-10625104 None:intergenic 35.0%
!! ATGATGGATAGTATCGAACT+GGG + chr1.3:10625422-10625441 None:intergenic 35.0%
!! TATGATGGATAGTATCGAAC+TGG + chr1.3:10625423-10625442 None:intergenic 35.0%
ATCCAGATTTCCAACTGGAT+CGG + chr1.3:10625645-10625664 None:intergenic 40.0%
ATGGAACCCAACAACTAAAC+AGG - chr1.3:10625173-10625192 MS.gene86273:CDS 40.0%
CCAGTTGGAAATCTGGATTA+TGG - chr1.3:10625647-10625666 MS.gene86273:CDS 40.0%
CCATAATCCAGATTTCCAAC+TGG + chr1.3:10625650-10625669 None:intergenic 40.0%
CGAAAACATTGCTGAGATCA+AGG - chr1.3:10625581-10625600 MS.gene86273:CDS 40.0%
CTCTTGGTCAATTGATCCTA+TGG - chr1.3:10625154-10625173 MS.gene86273:CDS 40.0%
GGAGGTTTGAATCCATCAAT+GGG + chr1.3:10624973-10624992 None:intergenic 40.0%
GGATTGATTACCAGGAAAGA+GGG + chr1.3:10625064-10625083 None:intergenic 40.0%
GTAGACCGAATCGAAACATA+TGG - chr1.3:10625084-10625103 MS.gene86273:CDS 40.0%
GTTTAGTTGTTGGGTTCCAT+AGG + chr1.3:10625173-10625192 None:intergenic 40.0%
TCACTTATGCAACATGTCCA+CGG + chr1.3:10625714-10625733 None:intergenic 40.0%
TCTGGATTATGGATCAGAAC+CGG - chr1.3:10625658-10625677 MS.gene86273:CDS 40.0%
TTAACAACCGGGGTATATGA+TGG + chr1.3:10625438-10625457 None:intergenic 40.0%
! TGGCCCAATGTTGTACATTT+TGG + chr1.3:10625688-10625707 None:intergenic 40.0%
!! TGTTGTACATTTTGGTCCAC+CGG + chr1.3:10625680-10625699 None:intergenic 40.0%
AAGGATGGCGATCAAGTCTT+TGG - chr1.3:10625741-10625760 MS.gene86273:CDS 45.0%
ACAGGAGAGAAGAGTGATGT+AGG + chr1.3:10624995-10625014 None:intergenic 45.0%
AGGTGGACCTGTTTAGTTGT+TGG + chr1.3:10625183-10625202 None:intergenic 45.0%
ATCACTCTTCTCTCCTGTGA+CGG - chr1.3:10624997-10625016 MS.gene86273:CDS 45.0%
ATTTCCAACTGGATCGGACT+AGG + chr1.3:10625639-10625658 None:intergenic 45.0%
CAGGAGAGAAGAGTGATGTA+GGG + chr1.3:10624994-10625013 None:intergenic 45.0%
CGGATTGATTACCAGGAAAG+AGG + chr1.3:10625065-10625084 None:intergenic 45.0%
CTGGCTTCATATGACGATCA+AGG - chr1.3:10625783-10625802 MS.gene86273:CDS 45.0%
GGGAGGTTTGAATCCATCAA+TGG + chr1.3:10624974-10624993 None:intergenic 45.0%
GGTGGACCTGTTTAGTTGTT+GGG + chr1.3:10625182-10625201 None:intergenic 45.0%
! AGAATGTTTAACGTGCCGAC+AGG + chr1.3:10625559-10625578 None:intergenic 45.0%
! CAAGTCTTTGGAGGAAAGGT+TGG - chr1.3:10625753-10625772 MS.gene86273:CDS 45.0%
! CGATCAAGTCTTTGGAGGAA+AGG - chr1.3:10625749-10625768 MS.gene86273:CDS 45.0%
! GAATGTTTAACGTGCCGACA+GGG + chr1.3:10625558-10625577 None:intergenic 45.0%
! GATAGTATCGAACTGGGATG+CGG + chr1.3:10625416-10625435 None:intergenic 45.0%
ACCAACTCAAAGCTCCCTGT+CGG - chr1.3:10625541-10625560 MS.gene86273:CDS 50.0%
ACTGGGATGCGGTTAAATGG+TGG + chr1.3:10625405-10625424 None:intergenic 50.0%
AGGAAAGGTTGGAAAGCTGC+TGG - chr1.3:10625764-10625783 MS.gene86273:CDS 50.0%
ATGCAACATGTCCACGGAAC+TGG + chr1.3:10625708-10625727 None:intergenic 50.0%
CGAACTGGGATGCGGTTAAA+TGG + chr1.3:10625408-10625427 None:intergenic 50.0%
GAGAGAAGAGTGATGTAGGG+AGG + chr1.3:10624991-10625010 None:intergenic 50.0%
GATGGCGATCAAGTCTTTGG+AGG - chr1.3:10625744-10625763 MS.gene86273:CDS 50.0%
GGATTATGGATCAGAACCGG+TGG - chr1.3:10625661-10625680 MS.gene86273:CDS 50.0%
GTCCGATCCAGTTGGAAATC+TGG - chr1.3:10625640-10625659 MS.gene86273:CDS 50.0%
TACTTCTGCTCCCTCTTTCC+TGG - chr1.3:10625051-10625070 MS.gene86273:CDS 50.0%
TCATACACTTCTGCGCCTGA+AGG + chr1.3:10625345-10625364 None:intergenic 50.0%
TCATCCTAGTCCGATCCAGT+TGG - chr1.3:10625632-10625651 MS.gene86273:CDS 50.0%
! AGTGACAGAATGAGGGTGCA+GGG + chr1.3:10625212-10625231 None:intergenic 50.0%
! CGGTCTACGGATTGATTACC+AGG + chr1.3:10625072-10625091 None:intergenic 50.0%
! GGATGCGGTTAAATGGTGGA+GGG + chr1.3:10625401-10625420 None:intergenic 50.0%
! GGGCTCCCTTGCTTATGTTT+TGG - chr1.3:10625602-10625621 MS.gene86273:CDS 50.0%
! GGTTAAATGGTGGAGGGTGA+TGG + chr1.3:10625395-10625414 None:intergenic 50.0%
ACAACATTGGGCCAGTTCCG+TGG - chr1.3:10625694-10625713 MS.gene86273:CDS 55.0%
ACTCTTCTCTCCTGTGACGG+TGG - chr1.3:10625000-10625019 MS.gene86273:CDS 55.0%
GGGATGCGGTTAAATGGTGG+AGG + chr1.3:10625402-10625421 None:intergenic 55.0%
! ATGAGGGTGCAGGGGGTATA+AGG + chr1.3:10625203-10625222 None:intergenic 55.0%
! GAGTGACAGAATGAGGGTGC+AGG + chr1.3:10625213-10625232 None:intergenic 55.0%
! GTGACAGAATGAGGGTGCAG+GGG + chr1.3:10625211-10625230 None:intergenic 55.0%
! TGACAGAATGAGGGTGCAGG+GGG + chr1.3:10625210-10625229 None:intergenic 55.0%
!! AATGGTGGAGGGTGATGGAC+AGG + chr1.3:10625390-10625409 None:intergenic 55.0%
!! ATGGTGGAGGGTGATGGACA+GGG + chr1.3:10625389-10625408 None:intergenic 55.0%
! AGGGTGCAGGGGGTATAAGG+TGG + chr1.3:10625200-10625219 None:intergenic 60.0%
!! GCCGACAGGGAGCTTTGAGT+TGG + chr1.3:10625545-10625564 None:intergenic 60.0%
!! TGGTGGAGGGTGATGGACAG+GGG + chr1.3:10625388-10625407 None:intergenic 60.0%
CTCTCCTGTGACGGTGGCGA+CGG - chr1.3:10625006-10625025 MS.gene86273:CDS 65.0%
TCCTGTGACGGTGGCGACGG+AGG - chr1.3:10625009-10625028 MS.gene86273:CDS 70.0%
TGGCGACGGAGGCGAGGAGT+TGG - chr1.3:10625020-10625039 MS.gene86273:CDS 70.0%
GCCTCCGTCGCCACCGTCAC+AGG + chr1.3:10625013-10625032 None:intergenic 75.0%
TGACGGTGGCGACGGAGGCG+AGG - chr1.3:10625014-10625033 MS.gene86273:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 10624955 10625902 10624955 ID=MS.gene86273
chr1.3 mRNA 10624955 10625902 10624955 ID=MS.gene86273.t1;Parent=MS.gene86273
chr1.3 exon 10624955 10625902 10624955 ID=MS.gene86273.t1.exon1;Parent=MS.gene86273.t1
chr1.3 CDS 10624955 10625902 10624955 ID=cds.MS.gene86273.t1;Parent=MS.gene86273.t1
Gene Sequence

>MS.gene86273

ATGATTTATCACAGACCCATTGATGGATTCAAACCTCCCTACATCACTCTTCTCTCCTGTGACGGTGGCGACGGAGGCGAGGAGTTGGAACGTATCTACTTCTGCTCCCTCTTTCCTGGTAATCAATCCGTAGACCGAATCGAAACATATGGCAATTGCAATGGTGTTTTCTTCTTAAAAGCTTTTTATAGGAATTCAACTCTTGGTCAATTGATCCTATGGAACCCAACAACTAAACAGGTCCACCTTATACCCCCTGCACCCTCATTCTGTCACTCTAAATATGATGATTCCCTGTATGGTTTCTGTGCATTTAACGACGACTGTAACATTAATTTCAAAGTTGTTAGACTTCAACAATGTGCACATGTTGAGAAGATGATTATTCCTTCAGGCGCAGAAGTGTATGATCTAAGCACTAAATCATGGACCCCTGTCCATCACCCTCCACCATTTAACCGCATCCCAGTTCGATACTATCCATCATATACCCCGGTTGTTAACTGTGTTTATCATTGGATTGTTACTGTCGATTTGCATACTACTTCAAACATCATCTGCTTTGACTTTCACAACAATCAGTTTCACCAACTCAAAGCTCCCTGTCGGCACGTTAAACATTCTAGCGAAAACATTGCTGAGATCAAGGGCTCCCTTGCTTATGTTTTGGAGTATCATCATCCTAGTCCGATCCAGTTGGAAATCTGGATTATGGATCAGAACCGGTGGACCAAAATGTACAACATTGGGCCAGTTCCGTGGACATGTTGCATAAGTGATTTCTGGAAGGATGGCGATCAAGTCTTTGGAGGAAAGGTTGGAAAGCTGCTGGCTTCATATGACGATCAAGGCAACTCGCTCAGCGAATTTCAAACTTATGTTTTGAATTACTATAGATGTTTATGGGGTTCTGAGTATTTACAGAGCATAACTCCCTTGTCAACCTAG
Protein sequence

>MS.gene86273.t1

MIYHRPIDGFKPPYITLLSCDGGDGGEELERIYFCSLFPGNQSVDRIETYGNCNGVFFLKAFYRNSTLGQLILWNPTTKQVHLIPPAPSFCHSKYDDSLYGFCAFNDDCNINFKVVRLQQCAHVEKMIIPSGAEVYDLSTKSWTPVHHPPPFNRIPVRYYPSYTPVVNCVYHWIVTVDLHTTSNIICFDFHNNQFHQLKAPCRHVKHSSENIAEIKGSLAYVLEYHHPSPIQLEIWIMDQNRWTKMYNIGPVPWTCCISDFWKDGDQVFGGKVGKLLASYDDQGNSLSEFQTYVLNYYRCLWGSEYLQSITPLST