AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58240


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58240.t1 MTR_8g433040 82.308 130 23 0 1 130 235 364 5.52e-70 214
MS.gene58240.t1 MTR_8g032380 82.308 130 23 0 1 130 235 364 5.52e-70 214
MS.gene58240.t1 MTR_8g433030 79.200 125 25 1 1 124 212 336 7.63e-65 200
MS.gene58240.t1 MTR_8g032390 79.200 125 25 1 1 124 245 369 1.72e-64 200
MS.gene58240.t1 MTR_4g094365 64.925 134 43 2 1 130 231 364 6.71e-50 162
MS.gene58240.t1 MTR_3g103020 63.433 134 44 3 1 130 246 378 1.79e-49 162
MS.gene58240.t1 MTR_4g109270 60.448 134 48 3 1 130 232 364 3.00e-46 153
MS.gene58240.t1 MTR_4g116320 61.538 130 45 2 1 126 126 254 3.67e-45 147
MS.gene58240.t1 MTR_6g060650 58.647 133 48 4 1 129 243 372 1.38e-43 146
MS.gene58240.t1 MTR_4g122850 62.687 134 45 4 1 130 232 364 2.23e-43 146
MS.gene58240.t1 MTR_5g097210 60.156 128 47 2 2 127 235 360 2.44e-42 143
MS.gene58240.t1 MTR_4g117720 58.647 133 43 4 1 124 242 371 5.01e-42 142
MS.gene58240.t1 MTR_8g040935 56.716 134 53 2 2 130 75 208 8.02e-42 137
MS.gene58240.t1 MTR_4g116330 56.296 135 54 2 1 130 246 380 1.68e-41 141
MS.gene58240.t1 MTR_1g055175 53.030 132 58 2 1 129 70 200 1.41e-40 134
MS.gene58240.t1 MTR_4g109280 58.088 136 47 6 1 130 228 359 1.60e-40 138
MS.gene58240.t1 MTR_4g023130 58.015 131 51 3 1 129 184 312 1.17e-39 135
MS.gene58240.t1 MTR_3g034180 58.647 133 49 4 1 130 360 489 1.91e-39 138
MS.gene58240.t1 MTR_4g117850 56.693 127 49 3 2 123 248 373 1.16e-38 134
MS.gene58240.t1 MTR_1g055165 52.632 133 59 2 1 130 187 318 1.26e-38 132
MS.gene58240.t1 MTR_4g023080 55.725 131 54 3 1 129 225 353 1.33e-38 133
MS.gene58240.t1 MTR_4g021785 54.545 132 56 3 1 130 226 355 4.02e-38 132
MS.gene58240.t1 MTR_4g023380 51.282 156 49 5 1 130 225 379 2.05e-37 130
MS.gene58240.t1 MTR_4g118360 55.469 128 48 5 8 129 1 125 4.96e-37 122
MS.gene58240.t1 MTR_4g118370 53.488 129 55 3 1 127 132 257 9.30e-37 126
MS.gene58240.t1 MTR_4g023090 52.857 140 49 5 1 124 240 378 1.78e-36 128
MS.gene58240.t1 MTR_6g463430 53.383 133 55 4 1 130 74 202 1.95e-36 124
MS.gene58240.t1 MTR_1g055135 51.825 137 57 4 1 130 241 375 5.48e-36 127
MS.gene58240.t1 MTR_4g023070 51.049 143 54 3 2 129 228 369 1.67e-35 125
MS.gene58240.t1 MTR_4g117870 52.344 128 58 2 5 130 141 267 4.51e-35 122
MS.gene58240.t1 MTR_5g070130 54.264 129 53 4 1 124 243 370 6.58e-35 124
MS.gene58240.t1 MTR_5g070080 54.331 127 52 4 2 124 255 379 2.09e-34 122
MS.gene58240.t1 MTR_1g055195 53.788 132 46 5 1 124 348 472 4.76e-34 123
MS.gene58240.t1 MTR_3g034160 56.489 131 53 3 1 129 246 374 1.89e-32 117
MS.gene58240.t1 MTR_4g023120 48.611 144 58 3 2 130 228 370 2.45e-32 117
MS.gene58240.t1 MTR_1g055250 48.872 133 62 3 2 130 140 270 3.59e-32 115
MS.gene58240.t1 MTR_4g118380 57.895 114 34 4 1 103 245 355 7.49e-32 115
MS.gene58240.t1 MTR_3g034130 54.167 120 50 3 1 117 246 363 7.68e-32 115
MS.gene58240.t1 MTR_4g117780 55.357 112 47 2 22 130 256 367 1.01e-31 115
MS.gene58240.t1 MTR_4g117750 54.386 114 49 2 18 128 368 481 1.03e-31 117
MS.gene58240.t1 MTR_4g117750 48.624 109 51 3 23 129 253 358 9.42e-24 95.1
MS.gene58240.t1 MTR_4g117770 55.357 112 47 2 22 130 256 367 1.46e-31 115
MS.gene58240.t1 MTR_4g117810 55.357 112 47 2 22 130 256 367 1.46e-31 115
MS.gene58240.t1 MTR_4g023190 52.206 136 54 4 1 128 225 357 1.70e-31 114
MS.gene58240.t1 MTR_5g023000 59.596 99 37 2 35 130 1 99 1.05e-30 105
MS.gene58240.t1 MTR_4g023150 50.000 136 57 4 1 128 226 358 1.66e-29 109
MS.gene58240.t1 MTR_4g023200 49.265 136 58 4 1 128 226 358 6.66e-29 107
MS.gene58240.t1 MTR_4g122860 50.000 128 54 4 10 130 196 320 2.97e-28 105
MS.gene58240.t1 MTR_4g116330 53.097 113 48 2 23 130 229 341 7.72e-28 104
MS.gene58240.t1 MTR_4g121580 50.847 118 49 3 4 120 230 339 3.02e-27 103
MS.gene58240.t1 MTR_4g023440 61.957 92 32 2 41 130 242 332 3.45e-27 102
MS.gene58240.t1 MTR_5g070088 46.667 135 67 4 1 130 252 386 3.74e-27 103
MS.gene58240.t1 MTR_4g117530 49.153 118 56 3 14 130 139 253 4.90e-27 100
MS.gene58240.t1 MTR_7g079400 45.652 138 63 5 2 130 203 337 1.12e-25 99.0
MS.gene58240.t1 MTR_8g102520 44.030 134 67 4 2 130 192 322 5.21e-25 97.1
MS.gene58240.t1 MTR_4g118400 49.219 128 44 6 2 124 183 294 7.19e-24 93.6
MS.gene58240.t1 MTR_7g079450 41.912 136 63 5 2 130 11 137 2.64e-23 88.6
MS.gene58240.t1 MTR_7g079370 45.669 127 62 4 2 124 227 350 3.32e-22 90.1
MS.gene58240.t1 MTR_8g098830 43.200 125 63 5 2 124 97 215 2.53e-21 85.5
MS.gene58240.t1 MTR_4g023430 46.667 105 48 3 33 130 119 222 9.27e-21 84.0
MS.gene58240.t1 MTR_4g122840 41.085 129 39 4 5 130 170 264 1.76e-17 76.3
MS.gene58240.t1 MTR_4g114700 50.000 88 38 3 40 121 131 218 3.57e-17 75.1
MS.gene58240.t1 MTR_7g117720 55.172 87 30 4 46 124 186 271 9.97e-17 74.3
MS.gene58240.t1 MTR_4g023100 48.718 78 37 1 53 130 45 119 2.09e-13 62.4
MS.gene58240.t1 MTR_8g013005 59.649 57 21 1 76 130 3 59 6.24e-13 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCATGATCCTAAGTATTA+TGG 0.203715 8.4:-64263239 MS.gene58240:CDS
TATTATGGTTTGTGGGTATT+TGG 0.206725 8.4:-64263224 MS.gene58240:CDS
ATAATCCGGCACTTGAAATA+TGG 0.211797 8.4:-64263169 MS.gene58240:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.288501 8.4:-64263205 MS.gene58240:CDS
ATGGCCACTTCAAATGCCTT+TGG 0.385262 8.4:+64262960 None:intergenic
CAAGGTATGTCATTGTCAAT+AGG 0.400804 8.4:+64263095 None:intergenic
GGATCATGGTTGAAATCATC+TGG 0.411154 8.4:+64263251 None:intergenic
ATCCTAAGTATTATGGTTTG+TGG 0.419854 8.4:-64263232 MS.gene58240:CDS
TAATCCGGCACTTGAAATAT+GGG 0.448185 8.4:-64263168 MS.gene58240:CDS
ATTGGAACTGGTGGTCATCA+TGG 0.466146 8.4:-64263035 MS.gene58240:CDS
AATACAAACTGAAATCATCT+TGG 0.466347 8.4:-64263136 MS.gene58240:CDS
ATCACCCATATTTCAAGTGC+CGG 0.473975 8.4:+64263164 None:intergenic
AGTGGTGATATTATTGGAAC+TGG 0.479168 8.4:-64263047 MS.gene58240:CDS
TCCTAAGTATTATGGTTTGT+GGG 0.484785 8.4:-64263231 MS.gene58240:CDS
AACCATAATACTTAGGATCA+TGG 0.502897 8.4:+64263237 None:intergenic
CTATTGACAATGACATACCT+TGG 0.516935 8.4:-64263094 MS.gene58240:CDS
TTGTCTTTGATTTAATGGAA+AGG 0.526787 8.4:-64263295 None:intergenic
ACCCACAAACCATAATACTT+AGG 0.570029 8.4:+64263230 None:intergenic
TGGTTGAAATCATCTGGAAG+AGG 0.588065 8.4:+64263257 None:intergenic
AACTGGTGGTCATCATGGAT+TGG 0.588954 8.4:-64263030 MS.gene58240:CDS
CATCTTGTTAAACTTGCTCA+CGG 0.601761 8.4:+64262904 None:intergenic
AAGCAGAGACTCTTTATACA+TGG 0.605588 8.4:+64262941 None:intergenic
GAATCCAAAGGCATTTGAAG+TGG 0.606386 8.4:-64262964 MS.gene58240:CDS
GCATCTCAAAATGAATCCAA+AGG 0.611920 8.4:-64262976 MS.gene58240:CDS
TAAACTTGCTCACGGTCACG+AGG 0.626552 8.4:+64262912 None:intergenic
TTGGTGAAGTATAATAACAA+AGG 0.635362 8.4:-64263011 MS.gene58240:CDS
GGTGATATTATTGGAACTGG+TGG 0.654879 8.4:-64263044 MS.gene58240:CDS
AAACTTGCTCACGGTCACGA+GGG 0.700858 8.4:+64262913 None:intergenic
GAGTATATAGGGGAAAACCA+AGG 0.732405 8.4:+64263077 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATATGAATTTTCATAATC+CGG - chr8.4:64263009-64263028 MS.gene58240:CDS 20.0%
! AATACAAACTGAAATCATCT+TGG - chr8.4:64263056-64263075 MS.gene58240:CDS 25.0%
! ACAAAAAGTGGTGATATTAT+TGG - chr8.4:64263139-64263158 MS.gene58240:CDS 25.0%
! TTGGTGAAGTATAATAACAA+AGG - chr8.4:64263181-64263200 MS.gene58240:CDS 25.0%
!! ACTTTTTGTAGAGTATATAG+GGG + chr8.4:64263128-64263147 None:intergenic 25.0%
!!! CACTTTTTGTAGAGTATATA+GGG + chr8.4:64263129-64263148 None:intergenic 25.0%
AACCATAATACTTAGGATCA+TGG + chr8.4:64262958-64262977 None:intergenic 30.0%
AACCATGATCCTAAGTATTA+TGG - chr8.4:64262953-64262972 MS.gene58240:CDS 30.0%
ATCCTAAGTATTATGGTTTG+TGG - chr8.4:64262960-64262979 MS.gene58240:CDS 30.0%
CCTATATACTCTACAAAAAG+TGG - chr8.4:64263127-64263146 MS.gene58240:CDS 30.0%
! TATTATGGTTTGTGGGTATT+TGG - chr8.4:64262968-64262987 MS.gene58240:CDS 30.0%
! TCCTAAGTATTATGGTTTGT+GGG - chr8.4:64262961-64262980 MS.gene58240:CDS 30.0%
!!! CCACTTTTTGTAGAGTATAT+AGG + chr8.4:64263130-64263149 None:intergenic 30.0%
AAGCAGAGACTCTTTATACA+TGG + chr8.4:64263254-64263273 None:intergenic 35.0%
ACCCACAAACCATAATACTT+AGG + chr8.4:64262965-64262984 None:intergenic 35.0%
AGTGGTGATATTATTGGAAC+TGG - chr8.4:64263145-64263164 MS.gene58240:CDS 35.0%
ATAATCCGGCACTTGAAATA+TGG - chr8.4:64263023-64263042 MS.gene58240:CDS 35.0%
CAAGGTATGTCATTGTCAAT+AGG + chr8.4:64263100-64263119 None:intergenic 35.0%
CTATTGACAATGACATACCT+TGG - chr8.4:64263098-64263117 MS.gene58240:CDS 35.0%
GCATCTCAAAATGAATCCAA+AGG - chr8.4:64263216-64263235 MS.gene58240:CDS 35.0%
TAATCCGGCACTTGAAATAT+GGG - chr8.4:64263024-64263043 MS.gene58240:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr8.4:64262987-64263006 MS.gene58240:CDS 35.0%
GAGTATATAGGGGAAAACCA+AGG + chr8.4:64263118-64263137 None:intergenic 40.0%
GGATCATGGTTGAAATCATC+TGG + chr8.4:64262944-64262963 None:intergenic 40.0%
GGTGATATTATTGGAACTGG+TGG - chr8.4:64263148-64263167 MS.gene58240:CDS 40.0%
TGGTTGAAATCATCTGGAAG+AGG + chr8.4:64262938-64262957 None:intergenic 40.0%
! ATCACCCATATTTCAAGTGC+CGG + chr8.4:64263031-64263050 None:intergenic 40.0%
!! GAATCCAAAGGCATTTGAAG+TGG - chr8.4:64263228-64263247 MS.gene58240:CDS 40.0%
AACTGGTGGTCATCATGGAT+TGG - chr8.4:64263162-64263181 MS.gene58240:CDS 45.0%
ATGGCCACTTCAAATGCCTT+TGG + chr8.4:64263235-64263254 None:intergenic 45.0%
ATTGGAACTGGTGGTCATCA+TGG - chr8.4:64263157-64263176 MS.gene58240:CDS 45.0%
! AAACTTGCTCACGGTCACGA+GGG + chr8.4:64263282-64263301 None:intergenic 50.0%
! TAAACTTGCTCACGGTCACG+AGG + chr8.4:64263283-64263302 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 64262911 64263303 64262911 ID=MS.gene58240
chr8.4 mRNA 64262911 64263303 64262911 ID=MS.gene58240.t1;Parent=MS.gene58240
chr8.4 exon 64262911 64263303 64262911 ID=MS.gene58240.t1.exon1;Parent=MS.gene58240.t1
chr8.4 CDS 64262911 64263303 64262911 ID=cds.MS.gene58240.t1;Parent=MS.gene58240.t1
Gene Sequence

>MS.gene58240

ATGGAAAGGAAACTGTTAGAGATACCTCTTCCAGATGATTTCAACCATGATCCTAAGTATTATGGTTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGAATATGAATTTTCATAATCCGGCACTTGAAATATGGGTGATGAAAGAATACAAACTGAAATCATCTTGGACTAAGACTCTTGTAATTCCTATTGACAATGACATACCTTGGTTTTCCCCTATATACTCTACAAAAAGTGGTGATATTATTGGAACTGGTGGTCATCATGGATTGGTGAAGTATAATAACAAAGGACAACTTTTAGAGCATCTCAAAATGAATCCAAAGGCATTTGAAGTGGCCATGTATAAAGAGTCTCTGCTTTCACTCCCTCGTGACCGTGAGCAAGTTTAA

Protein sequence

>MS.gene58240.t1

MERKLLEIPLPDDFNHDPKYYGLWVFGEFLSLWNMNFHNPALEIWVMKEYKLKSSWTKTLVIPIDNDIPWFSPIYSTKSGDIIGTGGHHGLVKYNNKGQLLEHLKMNPKAFEVAMYKESLLSLPRDREQV