AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57531


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57531.t1 MTR_8g433040 77.692 130 25 1 1 126 235 364 2.24e-64 199
MS.gene57531.t1 MTR_8g032380 77.692 130 25 1 1 126 235 364 2.24e-64 199
MS.gene57531.t1 MTR_8g433030 76.000 125 25 2 1 120 212 336 2.51e-58 183
MS.gene57531.t1 MTR_8g032390 76.000 125 25 2 1 120 245 369 5.18e-58 183
MS.gene57531.t1 MTR_3g103020 63.158 133 42 2 1 126 246 378 2.79e-48 159
MS.gene57531.t1 MTR_4g094365 62.500 136 39 4 1 126 231 364 1.90e-46 153
MS.gene57531.t1 MTR_6g060650 57.576 132 47 3 1 125 243 372 8.13e-44 147
MS.gene57531.t1 MTR_4g116330 57.778 135 48 2 1 126 246 380 9.75e-43 144
MS.gene57531.t1 MTR_4g109270 57.895 133 49 2 1 126 232 364 5.07e-42 142
MS.gene57531.t1 MTR_4g122850 62.500 136 38 6 1 126 232 364 8.16e-42 142
MS.gene57531.t1 MTR_8g040935 56.716 134 49 2 2 126 75 208 3.88e-41 135
MS.gene57531.t1 MTR_4g117720 58.647 133 41 4 1 121 242 372 1.90e-40 138
MS.gene57531.t1 MTR_1g055175 55.303 132 51 3 1 125 70 200 3.41e-40 133
MS.gene57531.t1 MTR_4g023130 57.692 130 49 2 1 125 184 312 4.95e-40 135
MS.gene57531.t1 MTR_6g463430 55.039 129 55 2 1 126 74 202 5.48e-40 132
MS.gene57531.t1 MTR_4g109280 57.778 135 45 5 1 126 228 359 8.38e-40 136
MS.gene57531.t1 MTR_4g023380 52.258 155 45 4 1 126 225 379 2.00e-39 135
MS.gene57531.t1 MTR_5g097210 59.690 129 42 4 2 123 235 360 3.37e-39 135
MS.gene57531.t1 MTR_4g116320 58.462 130 45 3 1 122 126 254 6.40e-39 131
MS.gene57531.t1 MTR_4g023090 53.061 147 48 4 1 126 240 386 1.68e-38 133
MS.gene57531.t1 MTR_3g034180 55.970 134 47 5 1 126 360 489 1.89e-38 135
MS.gene57531.t1 MTR_1g055165 54.887 133 52 3 1 126 187 318 2.08e-38 131
MS.gene57531.t1 MTR_4g023080 55.385 130 52 2 1 125 225 353 5.82e-38 131
MS.gene57531.t1 MTR_4g021785 54.198 131 54 2 1 126 226 355 1.46e-37 130
MS.gene57531.t1 MTR_4g117870 54.688 128 51 3 5 126 141 267 5.04e-36 124
MS.gene57531.t1 MTR_4g117850 54.762 126 49 2 2 119 248 373 1.68e-35 125
MS.gene57531.t1 MTR_4g023070 50.000 142 53 2 2 125 228 369 3.79e-35 124
MS.gene57531.t1 MTR_4g023120 48.951 143 55 2 2 126 228 370 3.40e-34 122
MS.gene57531.t1 MTR_1g055135 53.285 137 51 6 1 126 241 375 3.46e-34 122
MS.gene57531.t1 MTR_5g070130 54.688 128 50 3 1 120 243 370 3.78e-34 122
MS.gene57531.t1 MTR_4g118360 53.543 127 48 4 8 125 1 125 4.48e-34 115
MS.gene57531.t1 MTR_4g023190 53.383 133 53 3 1 124 225 357 1.59e-33 120
MS.gene57531.t1 MTR_4g118370 54.264 129 50 4 1 123 132 257 3.49e-33 116
MS.gene57531.t1 MTR_4g118380 58.407 113 31 3 1 99 245 355 3.65e-33 119
MS.gene57531.t1 MTR_5g070080 53.383 133 53 4 2 126 255 386 4.62e-32 116
MS.gene57531.t1 MTR_4g023150 51.128 133 56 3 1 124 226 358 9.00e-32 115
MS.gene57531.t1 MTR_3g034130 56.311 103 38 2 1 98 246 346 2.69e-31 114
MS.gene57531.t1 MTR_5g023000 60.606 99 32 2 35 126 1 99 3.51e-31 107
MS.gene57531.t1 MTR_4g117750 56.881 109 40 2 23 124 373 481 4.39e-31 115
MS.gene57531.t1 MTR_4g117750 50.000 108 47 3 23 125 253 358 2.02e-21 88.6
MS.gene57531.t1 MTR_4g023200 50.376 133 57 3 1 124 226 358 9.44e-31 112
MS.gene57531.t1 MTR_1g055195 51.852 135 44 6 1 122 348 474 1.03e-30 114
MS.gene57531.t1 MTR_3g034160 55.725 131 50 3 1 125 246 374 2.65e-30 111
MS.gene57531.t1 MTR_4g122860 50.000 128 50 3 10 126 196 320 4.66e-30 110
MS.gene57531.t1 MTR_4g117780 54.464 112 44 2 22 126 256 367 4.66e-30 110
MS.gene57531.t1 MTR_4g117770 54.464 112 44 2 22 126 256 367 6.25e-30 110
MS.gene57531.t1 MTR_4g117810 54.464 112 44 2 22 126 256 367 6.25e-30 110
MS.gene57531.t1 MTR_4g116330 54.867 113 42 2 23 126 229 341 4.43e-29 107
MS.gene57531.t1 MTR_1g055250 52.137 117 48 3 2 113 140 253 3.57e-28 105
MS.gene57531.t1 MTR_7g079400 48.905 137 56 4 2 126 203 337 1.63e-27 103
MS.gene57531.t1 MTR_7g079450 46.565 131 60 4 2 126 11 137 6.78e-27 97.4
MS.gene57531.t1 MTR_4g118400 50.000 126 42 3 2 120 183 294 3.06e-26 99.8
MS.gene57531.t1 MTR_4g121580 49.123 114 53 2 4 116 230 339 3.32e-26 100
MS.gene57531.t1 MTR_4g117530 48.305 118 53 3 14 126 139 253 9.32e-26 97.4
MS.gene57531.t1 MTR_4g023440 59.341 91 32 1 41 126 242 332 1.38e-25 98.6
MS.gene57531.t1 MTR_5g070088 45.926 135 64 4 1 126 252 386 2.69e-25 98.6
MS.gene57531.t1 MTR_8g102520 44.361 133 64 4 2 126 192 322 1.31e-24 95.9
MS.gene57531.t1 MTR_7g079370 46.457 127 59 4 2 121 227 351 2.57e-23 92.8
MS.gene57531.t1 MTR_4g023430 46.154 104 46 2 33 126 119 222 8.36e-21 84.0
MS.gene57531.t1 MTR_4g122840 42.400 125 39 4 5 126 170 264 1.64e-20 84.0
MS.gene57531.t1 MTR_7g117720 56.322 87 27 3 46 121 186 272 4.11e-20 83.2
MS.gene57531.t1 MTR_8g098830 41.935 124 62 5 2 120 97 215 5.69e-20 82.0
MS.gene57531.t1 MTR_7g079420 41.791 134 67 6 2 126 1013 1144 1.48e-17 77.8
MS.gene57531.t1 MTR_4g114700 50.575 87 33 3 41 117 132 218 5.57e-16 71.6
MS.gene57531.t1 MTR_4g023100 61.818 55 21 0 72 126 65 119 8.14e-15 65.9
MS.gene57531.t1 MTR_2g048580 36.641 131 69 6 2 126 220 342 1.03e-13 66.2
MS.gene57531.t1 MTR_8g013005 59.649 57 21 1 72 126 3 59 2.52e-13 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 28 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTATGGTTTGTGGGTATT+TGG 0.222701 8.3:-62060398 MS.gene57531:CDS
ATAATTGGGCAGTTGAAATA+TGG 0.222963 8.3:-62060343 MS.gene57531:CDS
AACCATGATCCTGTGCATTA+TGG 0.250864 8.3:-62060413 MS.gene57531:CDS
TTGGAGAATTTCTTAGTCTA+TGG 0.272823 8.3:-62060379 MS.gene57531:CDS
ATGGCCACTTCAAATGCATT+TGG 0.358614 8.3:+62060146 None:intergenic
AGTATATAGGGGAAAAGTTA+AGG 0.407366 8.3:+62060264 None:intergenic
ATCCTGTGCATTATGGTTTG+TGG 0.434302 8.3:-62060406 MS.gene57531:CDS
TTGGTGAAGTATAATATTAA+AGG 0.434508 8.3:-62060197 MS.gene57531:CDS
ACTGTTAGAGATACCTCTTC+CGG 0.452548 8.3:-62060444 MS.gene57531:CDS
TAATTGGGCAGTTGAAATAT+GGG 0.461070 8.3:-62060342 MS.gene57531:CDS
TCCTGTGCATTATGGTTTGT+GGG 0.467589 8.3:-62060405 MS.gene57531:CDS
TAAACTTGCTCACGGTCACT+AGG 0.478802 8.3:+62060098 None:intergenic
GGATCATGGTTAAAATCATC+CGG 0.492599 8.3:+62060425 None:intergenic
AATACAAACAACATTCGTCT+TGG 0.503398 8.3:-62060310 MS.gene57531:CDS
TTGTCTTTGATTTAATGGAA+AGG 0.526787 8.3:-62060469 None:intergenic
AACTGATGGTGGTCGTGGAT+TGG 0.526792 8.3:-62060216 MS.gene57531:CDS
AGTTGAAATATGGGTGATGA+AGG 0.529420 8.3:-62060333 MS.gene57531:CDS
GTTGGAACTGATGGTGGTCG+TGG 0.543560 8.3:-62060221 MS.gene57531:CDS
AACCATAATGCACAGGATCA+TGG 0.559016 8.3:+62060411 None:intergenic
ATAGGGGAAAAGTTAAGGCT+AGG 0.562080 8.3:+62060269 None:intergenic
GAATCCAAATGCATTTGAAG+TGG 0.564657 8.3:-62060150 MS.gene57531:CDS
AAGCAGAGACTCTGTATACA+TGG 0.569558 8.3:+62060127 None:intergenic
TGGTTAAAATCATCCGGAAG+AGG 0.573708 8.3:+62060431 None:intergenic
ACCCACAAACCATAATGCAC+AGG 0.579215 8.3:+62060404 None:intergenic
GGTGATATTGTTGGAACTGA+TGG 0.579963 8.3:-62060230 MS.gene57531:CDS
AAACTTGCTCACGGTCACTA+GGG 0.598587 8.3:+62060099 None:intergenic
TATCTTGTTAAACTTGCTCA+CGG 0.621078 8.3:+62060090 None:intergenic
GATATTGTTGGAACTGATGG+TGG 0.641384 8.3:-62060227 MS.gene57531:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATTTGAATTTTCATAATT+GGG - chr8.3:62060195-62060214 MS.gene57531:CDS 15.0%
!! TTGGTGAAGTATAATATTAA+AGG - chr8.3:62060355-62060374 MS.gene57531:CDS 20.0%
!!! GGAATTTGAATTTTCATAAT+TGG - chr8.3:62060194-62060213 MS.gene57531:CDS 20.0%
!! ACTTTTTGTAGAGTATATAG+GGG + chr8.3:62060302-62060321 None:intergenic 25.0%
!!! CACTTTTTGTAGAGTATATA+GGG + chr8.3:62060303-62060322 None:intergenic 25.0%
AATACAAACAACATTCGTCT+TGG - chr8.3:62060242-62060261 MS.gene57531:CDS 30.0%
ACAAAAAGTGGTGATATTGT+TGG - chr8.3:62060313-62060332 MS.gene57531:CDS 30.0%
AGTATATAGGGGAAAAGTTA+AGG + chr8.3:62060291-62060310 None:intergenic 30.0%
ATAATTGGGCAGTTGAAATA+TGG - chr8.3:62060209-62060228 MS.gene57531:CDS 30.0%
CCTATATACTCTACAAAAAG+TGG - chr8.3:62060301-62060320 MS.gene57531:CDS 30.0%
TAATTGGGCAGTTGAAATAT+GGG - chr8.3:62060210-62060229 MS.gene57531:CDS 30.0%
TTGGAGAATTTCTTAGTCTA+TGG - chr8.3:62060173-62060192 MS.gene57531:CDS 30.0%
!!! CCACTTTTTGTAGAGTATAT+AGG + chr8.3:62060304-62060323 None:intergenic 30.0%
AGTTGAAATATGGGTGATGA+AGG - chr8.3:62060219-62060238 MS.gene57531:CDS 35.0%
GAATCCAAATGCATTTGAAG+TGG - chr8.3:62060402-62060421 MS.gene57531:CDS 35.0%
GGATCATGGTTAAAATCATC+CGG + chr8.3:62060130-62060149 None:intergenic 35.0%
! CATTATGGTTTGTGGGTATT+TGG - chr8.3:62060154-62060173 MS.gene57531:CDS 35.0%
AACCATAATGCACAGGATCA+TGG + chr8.3:62060144-62060163 None:intergenic 40.0%
AACCATGATCCTGTGCATTA+TGG - chr8.3:62060139-62060158 MS.gene57531:CDS 40.0%
AAGCAGAGACTCTGTATACA+TGG + chr8.3:62060428-62060447 None:intergenic 40.0%
ACTGTTAGAGATACCTCTTC+CGG - chr8.3:62060108-62060127 MS.gene57531:CDS 40.0%
ATAGGGGAAAAGTTAAGGCT+AGG + chr8.3:62060286-62060305 None:intergenic 40.0%
ATCCTGTGCATTATGGTTTG+TGG - chr8.3:62060146-62060165 MS.gene57531:CDS 40.0%
ATGGCCACTTCAAATGCATT+TGG + chr8.3:62060409-62060428 None:intergenic 40.0%
GATATTGTTGGAACTGATGG+TGG - chr8.3:62060325-62060344 MS.gene57531:CDS 40.0%
GGTGATATTGTTGGAACTGA+TGG - chr8.3:62060322-62060341 MS.gene57531:CDS 40.0%
TGGTTAAAATCATCCGGAAG+AGG + chr8.3:62060124-62060143 None:intergenic 40.0%
! TCCTGTGCATTATGGTTTGT+GGG - chr8.3:62060147-62060166 MS.gene57531:CDS 40.0%
ACCCACAAACCATAATGCAC+AGG + chr8.3:62060151-62060170 None:intergenic 45.0%
! AAACTTGCTCACGGTCACTA+GGG + chr8.3:62060456-62060475 None:intergenic 45.0%
! TAAACTTGCTCACGGTCACT+AGG + chr8.3:62060457-62060476 None:intergenic 45.0%
!! AACTGATGGTGGTCGTGGAT+TGG - chr8.3:62060336-62060355 MS.gene57531:CDS 50.0%
!! GTTGGAACTGATGGTGGTCG+TGG - chr8.3:62060331-62060350 MS.gene57531:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 62060097 62060477 62060097 ID=MS.gene57531
chr8.3 mRNA 62060097 62060477 62060097 ID=MS.gene57531.t1;Parent=MS.gene57531
chr8.3 exon 62060097 62060477 62060097 ID=MS.gene57531.t1.exon1;Parent=MS.gene57531.t1
chr8.3 CDS 62060097 62060477 62060097 ID=cds.MS.gene57531.t1;Parent=MS.gene57531.t1
Gene Sequence

>MS.gene57531

ATGGAAAGGAAACTGTTAGAGATACCTCTTCCGGATGATTTTAACCATGATCCTGTGCATTATGGTTTGTGGGTATTTGGAGAATTTCTTAGTCTATGGAATTTGAATTTTCATAATTGGGCAGTTGAAATATGGGTGATGAAGGAATACAAACAACATTCGTCTTGGACTAAGTCTCTTGTAATTCCTAGCCTTAACTTTTCCCCTATATACTCTACAAAAAGTGGTGATATTGTTGGAACTGATGGTGGTCGTGGATTGGTGAAGTATAATATTAAAGGACAGCTGTTAGAGTATCTTGAAATGAATCCAAATGCATTTGAAGTGGCCATGTATACAGAGTCTCTGCTTTCACTCCCTAGTGACCGTGAGCAAGTTTAA

Protein sequence

>MS.gene57531.t1

MERKLLEIPLPDDFNHDPVHYGLWVFGEFLSLWNLNFHNWAVEIWVMKEYKQHSSWTKSLVIPSLNFSPIYSTKSGDIVGTDGGRGLVKYNIKGQLLEYLEMNPNAFEVAMYTESLLSLPSDREQV