AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68890.t1 MTR_4g023090 66.667 225 53 5 2 207 165 386 1.67e-95 283
MS.gene68890.t1 MTR_4g023380 64.530 234 38 5 1 207 164 379 3.02e-93 277
MS.gene68890.t1 MTR_4g023130 67.773 211 54 4 1 206 111 312 8.42e-89 264
MS.gene68890.t1 MTR_4g023080 68.246 211 53 4 1 206 152 353 3.71e-88 263
MS.gene68890.t1 MTR_4g021785 64.789 213 60 5 1 207 152 355 1.47e-81 247
MS.gene68890.t1 MTR_4g023070 58.667 225 73 5 1 206 146 369 6.78e-80 243
MS.gene68890.t1 MTR_4g023120 57.778 225 77 4 1 207 146 370 4.19e-78 238
MS.gene68890.t1 MTR_4g023440 60.664 211 38 5 1 207 163 332 6.15e-74 226
MS.gene68890.t1 MTR_4g116330 58.605 215 76 6 2 207 170 380 1.67e-73 227
MS.gene68890.t1 MTR_3g103020 57.674 215 79 6 3 207 166 378 3.20e-72 224
MS.gene68890.t1 MTR_8g040935 55.340 206 79 4 14 207 4 208 4.50e-69 210
MS.gene68890.t1 MTR_6g060650 54.460 213 86 6 1 206 164 372 8.26e-69 214
MS.gene68890.t1 MTR_3g034180 56.250 224 77 8 1 207 270 489 4.13e-68 216
MS.gene68890.t1 MTR_4g109270 55.607 214 78 5 3 207 159 364 4.52e-68 213
MS.gene68890.t1 MTR_4g117720 56.109 221 79 8 1 207 162 378 4.63e-67 210
MS.gene68890.t1 MTR_4g023150 56.604 212 78 6 1 205 154 358 5.25e-67 209
MS.gene68890.t1 MTR_4g023200 56.604 212 78 6 1 205 154 358 6.52e-67 209
MS.gene68890.t1 MTR_4g116320 57.209 215 73 8 3 204 47 255 1.07e-66 206
MS.gene68890.t1 MTR_4g094365 54.128 218 81 7 1 207 155 364 3.85e-66 207
MS.gene68890.t1 MTR_4g023190 56.132 212 78 7 1 205 154 357 4.69e-65 204
MS.gene68890.t1 MTR_3g034160 55.204 221 80 7 2 206 157 374 1.71e-64 204
MS.gene68890.t1 MTR_4g122850 55.251 219 82 10 1 207 150 364 1.71e-63 201
MS.gene68890.t1 MTR_4g117770 54.419 215 83 5 3 207 158 367 3.72e-63 200
MS.gene68890.t1 MTR_4g117810 54.419 215 83 5 3 207 158 367 3.72e-63 200
MS.gene68890.t1 MTR_4g109280 54.167 216 85 7 2 207 148 359 1.95e-62 198
MS.gene68890.t1 MTR_8g433030 55.981 209 81 8 2 202 132 337 3.49e-62 197
MS.gene68890.t1 MTR_8g032390 55.981 209 81 8 2 202 165 370 9.47e-62 196
MS.gene68890.t1 MTR_3g034130 54.187 203 75 6 1 188 156 355 2.07e-60 193
MS.gene68890.t1 MTR_4g117780 53.023 215 86 5 3 207 158 367 2.43e-60 193
MS.gene68890.t1 MTR_6g463430 53.807 197 78 4 19 207 11 202 1.09e-59 186
MS.gene68890.t1 MTR_4g117850 53.953 215 84 9 3 204 164 376 7.64e-59 189
MS.gene68890.t1 MTR_8g433040 52.093 215 82 6 1 207 163 364 4.75e-58 187
MS.gene68890.t1 MTR_8g032380 52.093 215 82 6 1 207 163 364 4.75e-58 187
MS.gene68890.t1 MTR_4g118380 52.308 195 78 5 1 183 166 357 1.36e-55 180
MS.gene68890.t1 MTR_5g070130 47.982 223 99 5 1 207 156 377 2.37e-54 178
MS.gene68890.t1 MTR_5g070080 51.389 216 93 6 1 207 174 386 2.17e-53 175
MS.gene68890.t1 MTR_5g097210 50.926 216 87 10 1 203 150 359 1.26e-52 173
MS.gene68890.t1 MTR_4g117870 46.009 213 72 7 1 207 92 267 6.21e-51 166
MS.gene68890.t1 MTR_4g118400 48.259 201 82 5 8 201 109 294 1.11e-50 166
MS.gene68890.t1 MTR_1g055195 47.085 223 89 7 1 207 269 478 2.86e-50 169
MS.gene68890.t1 MTR_4g117530 44.976 209 73 4 3 207 83 253 4.90e-50 163
MS.gene68890.t1 MTR_4g116330 47.442 215 61 6 2 207 170 341 4.86e-49 163
MS.gene68890.t1 MTR_5g070088 46.544 217 106 4 1 207 170 386 2.04e-48 162
MS.gene68890.t1 MTR_1g055175 47.208 197 87 7 23 206 8 200 1.08e-46 152
MS.gene68890.t1 MTR_1g055135 44.105 229 97 9 1 207 156 375 1.36e-45 155
MS.gene68890.t1 MTR_1g055250 44.860 214 87 9 1 197 56 255 4.19e-45 152
MS.gene68890.t1 MTR_4g117750 44.196 224 103 8 1 206 139 358 6.45e-43 150
MS.gene68890.t1 MTR_4g117750 53.913 115 50 1 94 205 367 481 4.19e-31 118
MS.gene68890.t1 MTR_4g118370 42.593 216 90 10 1 204 64 257 5.31e-42 142
MS.gene68890.t1 MTR_1g055165 46.073 191 85 7 22 207 141 318 7.64e-42 144
MS.gene68890.t1 MTR_7g079370 42.593 216 105 6 1 201 139 350 3.33e-40 140
MS.gene68890.t1 MTR_5g023000 65.657 99 31 1 112 207 1 99 1.40e-39 131
MS.gene68890.t1 MTR_4g118360 57.364 129 45 5 84 206 1 125 3.67e-37 126
MS.gene68890.t1 MTR_8g102520 39.631 217 114 6 4 207 110 322 1.33e-36 130
MS.gene68890.t1 MTR_7g079400 39.111 225 114 7 1 207 118 337 1.88e-36 130
MS.gene68890.t1 MTR_4g121580 43.204 206 78 7 1 197 164 339 3.23e-36 130
MS.gene68890.t1 MTR_4g122860 39.352 216 87 8 9 207 132 320 8.57e-32 118
MS.gene68890.t1 MTR_8g098830 42.012 169 84 6 20 179 31 194 7.55e-31 113
MS.gene68890.t1 MTR_4g114700 39.524 210 64 7 2 198 59 218 4.34e-29 108
MS.gene68890.t1 MTR_7g079450 44.755 143 68 5 69 207 2 137 4.94e-28 103
MS.gene68890.t1 MTR_4g023430 65.854 82 22 2 132 207 141 222 1.78e-26 101
MS.gene68890.t1 MTR_4g117430 51.304 115 45 4 1 112 49 155 8.51e-26 97.8
MS.gene68890.t1 MTR_7g117720 63.218 87 25 2 123 202 186 272 6.34e-25 98.6
MS.gene68890.t1 MTR_2g048580 35.859 198 100 9 24 207 158 342 9.72e-25 99.4
MS.gene68890.t1 MTR_7g079420 37.705 183 95 7 38 207 968 1144 4.36e-24 99.8
MS.gene68890.t1 MTR_8g013010 50.442 113 46 4 1 109 203 309 1.52e-22 93.2
MS.gene68890.t1 MTR_4g023100 80.000 55 10 1 154 207 65 119 2.96e-21 85.1
MS.gene68890.t1 MTR_8g013005 62.069 58 19 1 152 206 1 58 2.20e-17 73.2
MS.gene68890.t1 MTR_4g122840 39.706 136 44 3 75 207 164 264 5.83e-17 77.0
MS.gene68890.t1 MTR_6g034720 45.556 90 44 2 1 85 80 169 2.90e-14 68.9
MS.gene68890.t1 MTR_4g116340 51.948 77 32 3 2 74 172 247 1.20e-13 67.8
MS.gene68890.t1 MTR_1g094210 26.667 195 122 7 25 207 221 406 2.42e-13 68.2
MS.gene68890.t1 MTR_2g025420 28.571 175 108 7 10 171 186 356 2.24e-12 65.5
MS.gene68890.t1 MTR_1g094210 27.564 156 100 5 25 173 221 370 2.47e-12 65.1
MS.gene68890.t1 MTR_7g017170 31.333 150 81 8 25 162 213 352 2.79e-12 65.1
MS.gene68890.t1 MTR_7g017130 31.333 150 81 8 25 162 213 352 3.43e-12 64.7
MS.gene68890.t1 MTR_1g068835 28.144 167 111 6 3 161 150 315 6.91e-12 63.9
MS.gene68890.t1 MTR_2g426020 24.631 203 138 6 4 200 171 364 8.00e-12 63.9
MS.gene68890.t1 MTR_1g055145 46.667 75 37 2 52 126 131 202 8.62e-12 63.2
MS.gene68890.t1 MTR_8g099460 27.742 155 100 6 17 168 178 323 1.05e-11 63.5
MS.gene68890.t1 MTR_2g028160 30.822 146 90 6 23 158 194 338 1.31e-11 63.2
MS.gene68890.t1 MTR_2g021150 29.167 168 104 9 3 161 167 328 2.45e-11 62.4
MS.gene68890.t1 MTR_2g028130 29.861 144 92 5 23 158 194 336 3.06e-11 62.0
MS.gene68890.t1 MTR_2g024110 25.153 163 112 5 1 153 147 309 4.06e-11 61.6
MS.gene68890.t1 MTR_3g007360 28.241 216 122 10 3 200 203 403 6.59e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68890.t1 AT3G06240 28.977 176 110 6 3 173 223 388 1.38e-13 68.9

Find 46 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGTTGCCTTTGATTTAA+TGG 0.113056 4.3:+74924037 MS.gene68890:CDS
CATACTGGTTTGTGGGTATT+TGG 0.151877 4.3:+74924116 MS.gene68890:CDS
AGAAAAGGGTTGCTATTTAA+TGG 0.223877 4.3:+74923969 MS.gene68890:CDS
CTAATGATACAATTGAAATA+TGG 0.264279 4.3:+74924171 MS.gene68890:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.267319 4.3:+74924135 MS.gene68890:CDS
GAAAAGGGTTGCTATTTAAT+GGG 0.297162 4.3:+74923970 MS.gene68890:CDS
AAAGAAATTCACTTCCCTTA+TGG 0.300752 4.3:+74923930 MS.gene68890:CDS
ATGATAAAGGACAGTTATTA+AGG 0.300794 4.3:+74924345 MS.gene68890:CDS
ATAATCAGATGGACTATTAA+AGG 0.324115 4.3:-74924375 None:intergenic
TTGGCTTTGCGTGAAGATTT+AGG 0.342328 4.3:+74924005 MS.gene68890:CDS
TATTTAATGGGGTTATTCAT+TGG 0.346478 4.3:+74923982 MS.gene68890:CDS
TGTTGTAACAGCATAAAGTA+TGG 0.380139 4.3:-74923871 None:intergenic
ATCTGATTATGTCATGTATA+CGG 0.391652 4.3:+74924388 MS.gene68890:CDS
GTCATCTCTTCTGTCAGAAA+AGG 0.411814 4.3:+74923954 MS.gene68890:CDS
TTGGCGAAGTATAATGATAA+AGG 0.416108 4.3:+74924332 MS.gene68890:CDS
ATAGGGCGAAAGCAGGGTTC+AGG 0.417607 4.3:-74924260 None:intergenic
TGTTCAGAATCACTTGGAAT+AGG 0.431059 4.3:-74924080 None:intergenic
TAATGGGGTTATTCATTGGT+TGG 0.432729 4.3:+74923986 MS.gene68890:CDS
CACAGAGCATTAAGACATAC+TGG 0.435069 4.3:+74924101 MS.gene68890:CDS
AATACAATGTGCATTCATCT+TGG 0.441546 4.3:+74924204 MS.gene68890:CDS
GATATTATTGGAATAAGTCC+TGG 0.455002 4.3:+74924302 MS.gene68890:CDS
TAATGATACAATTGAAATAT+GGG 0.469242 4.3:+74924172 MS.gene68890:CDS
CTTTCTTTCCATTAAATCAA+AGG 0.478029 4.3:-74924045 None:intergenic
ACAGAAGAGATGACACCATA+AGG 0.478205 4.3:-74923945 None:intergenic
CCAGTCAAGAGATGATTACT+TGG 0.479956 4.3:+74923824 None:intergenic
ATTAAGACATACTGGTTTGT+GGG 0.486970 4.3:+74924109 MS.gene68890:CDS
TGGAGAATTTCTCAGTCTAT+GGG 0.490268 4.3:+74924136 MS.gene68890:CDS
AATAAGTCCTGGGACTAGAT+TGG 0.496773 4.3:+74924313 MS.gene68890:CDS
CATTAAGACATACTGGTTTG+TGG 0.501728 4.3:+74924108 MS.gene68890:CDS
ATTTCTCAGTCTATGGGCTA+TGG 0.507502 4.3:+74924142 MS.gene68890:CDS
GAGTCTCTGCTTTCACTCCC+TGG 0.511723 4.3:+74924410 MS.gene68890:CDS
CAGAAGAGATGACACCATAA+GGG 0.519654 4.3:-74923944 None:intergenic
CTTTGCGTGAAGATTTAGGT+TGG 0.523190 4.3:+74924009 MS.gene68890:CDS
TCATCTCTTCTGTCAGAAAA+GGG 0.526425 4.3:+74923955 MS.gene68890:CDS
TAAACTTGCTCATTGTCACC+AGG 0.534960 4.3:-74924428 None:intergenic
GTCAAGAGATGATTACTTGG+TGG 0.535085 4.3:+74923827 None:intergenic
AGAACATATAGGGCGAAAGC+AGG 0.538165 4.3:-74924267 None:intergenic
TCTCATTTAGAGATAATACA+TGG 0.548869 4.3:+74923907 MS.gene68890:CDS
TACTTCGCCAATCTAGTCCC+AGG 0.568427 4.3:-74924320 None:intergenic
ATATTATTGGAATAAGTCCT+GGG 0.574033 4.3:+74924303 MS.gene68890:CDS
AAAAGGGTTGCTATTTAATG+GGG 0.580733 4.3:+74923971 MS.gene68890:CDS
GCGAAAGCAGGGTTCAGGAA+TGG 0.589429 4.3:-74924255 None:intergenic
GTATACATGACATAATCAGA+TGG 0.633695 4.3:-74924386 None:intergenic
GAACATATAGGGCGAAAGCA+GGG 0.645510 4.3:-74924266 None:intergenic
GCTCTGTGTTCAGAATCACT+TGG 0.661251 4.3:-74924086 None:intergenic
AAACTTGCTCATTGTCACCA+GGG 0.667535 4.3:-74924427 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGATACAATTGAAATAT+GGG + chr4.3:74924172-74924191 MS.gene68890:CDS 15.0%
!! ACAAAAAATGGTGATATTAT+TGG + chr4.3:74924290-74924309 MS.gene68890:CDS 20.0%
!! CTAATGATACAATTGAAATA+TGG + chr4.3:74924171-74924190 MS.gene68890:CDS 20.0%
!!! CATTTTTTGTAGAACATATA+GGG - chr4.3:74924280-74924299 None:intergenic 20.0%
! ATATTATTGGAATAAGTCCT+GGG + chr4.3:74924303-74924322 MS.gene68890:CDS 25.0%
! ATCTGATTATGTCATGTATA+CGG + chr4.3:74924388-74924407 MS.gene68890:CDS 25.0%
! ATGATAAAGGACAGTTATTA+AGG + chr4.3:74924345-74924364 MS.gene68890:CDS 25.0%
! CCTATATGTTCTACAAAAAA+TGG + chr4.3:74924278-74924297 MS.gene68890:CDS 25.0%
! CTTTCTTTCCATTAAATCAA+AGG - chr4.3:74924048-74924067 None:intergenic 25.0%
! TATTTAATGGGGTTATTCAT+TGG + chr4.3:74923982-74924001 MS.gene68890:CDS 25.0%
! TCTCATTTAGAGATAATACA+TGG + chr4.3:74923907-74923926 MS.gene68890:CDS 25.0%
!!! ATAATCAGATGGACTATTAA+AGG - chr4.3:74924378-74924397 None:intergenic 25.0%
!!! CCATTTTTTGTAGAACATAT+AGG - chr4.3:74924281-74924300 None:intergenic 25.0%
AAAGAAATTCACTTCCCTTA+TGG + chr4.3:74923930-74923949 MS.gene68890:CDS 30.0%
AATACAATGTGCATTCATCT+TGG + chr4.3:74924204-74924223 MS.gene68890:CDS 30.0%
GATATTATTGGAATAAGTCC+TGG + chr4.3:74924302-74924321 MS.gene68890:CDS 30.0%
GTATACATGACATAATCAGA+TGG - chr4.3:74924389-74924408 None:intergenic 30.0%
TGTTGTAACAGCATAAAGTA+TGG - chr4.3:74923874-74923893 None:intergenic 30.0%
TTGGCGAAGTATAATGATAA+AGG + chr4.3:74924332-74924351 MS.gene68890:CDS 30.0%
! CATTGTTGCCTTTGATTTAA+TGG + chr4.3:74924037-74924056 MS.gene68890:CDS 30.0%
!! AAAAGGGTTGCTATTTAATG+GGG + chr4.3:74923971-74923990 MS.gene68890:CDS 30.0%
!! AGAAAAGGGTTGCTATTTAA+TGG + chr4.3:74923969-74923988 MS.gene68890:CDS 30.0%
!! ATTAAGACATACTGGTTTGT+GGG + chr4.3:74924109-74924128 MS.gene68890:CDS 30.0%
!! GAAAAGGGTTGCTATTTAAT+GGG + chr4.3:74923970-74923989 MS.gene68890:CDS 30.0%
TAATGGGGTTATTCATTGGT+TGG + chr4.3:74923986-74924005 MS.gene68890:CDS 35.0%
TCATCTCTTCTGTCAGAAAA+GGG + chr4.3:74923955-74923974 MS.gene68890:CDS 35.0%
TGGAGAATTTCTCAGTCTAT+GGG + chr4.3:74924136-74924155 MS.gene68890:CDS 35.0%
TGTTCAGAATCACTTGGAAT+AGG - chr4.3:74924083-74924102 None:intergenic 35.0%
TTGGAGAATTTCTCAGTCTA+TGG + chr4.3:74924135-74924154 MS.gene68890:CDS 35.0%
!! CATTAAGACATACTGGTTTG+TGG + chr4.3:74924108-74924127 MS.gene68890:CDS 35.0%
AATAAGTCCTGGGACTAGAT+TGG + chr4.3:74924313-74924332 MS.gene68890:CDS 40.0%
ACAGAAGAGATGACACCATA+AGG - chr4.3:74923948-74923967 None:intergenic 40.0%
CAGAAGAGATGACACCATAA+GGG - chr4.3:74923947-74923966 None:intergenic 40.0%
CTTTGCGTGAAGATTTAGGT+TGG + chr4.3:74924009-74924028 MS.gene68890:CDS 40.0%
GTCATCTCTTCTGTCAGAAA+AGG + chr4.3:74923954-74923973 MS.gene68890:CDS 40.0%
! AAACTTGCTCATTGTCACCA+GGG - chr4.3:74924430-74924449 None:intergenic 40.0%
! ATTTCTCAGTCTATGGGCTA+TGG + chr4.3:74924142-74924161 MS.gene68890:CDS 40.0%
! CACAGAGCATTAAGACATAC+TGG + chr4.3:74924101-74924120 MS.gene68890:CDS 40.0%
! TAAACTTGCTCATTGTCACC+AGG - chr4.3:74924431-74924450 None:intergenic 40.0%
!! CATACTGGTTTGTGGGTATT+TGG + chr4.3:74924116-74924135 MS.gene68890:CDS 40.0%
!! TTGGCTTTGCGTGAAGATTT+AGG + chr4.3:74924005-74924024 MS.gene68890:CDS 40.0%
AGAACATATAGGGCGAAAGC+AGG - chr4.3:74924270-74924289 None:intergenic 45.0%
GAACATATAGGGCGAAAGCA+GGG - chr4.3:74924269-74924288 None:intergenic 45.0%
GCTCTGTGTTCAGAATCACT+TGG - chr4.3:74924089-74924108 None:intergenic 45.0%
TACTTCGCCAATCTAGTCCC+AGG - chr4.3:74924323-74924342 None:intergenic 50.0%
ATAGGGCGAAAGCAGGGTTC+AGG - chr4.3:74924263-74924282 None:intergenic 55.0%
GAGTCTCTGCTTTCACTCCC+TGG + chr4.3:74924410-74924429 MS.gene68890:CDS 55.0%
GCGAAAGCAGGGTTCAGGAA+TGG - chr4.3:74924258-74924277 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 74923828 74924451 74923828 ID=MS.gene68890
chr4.3 mRNA 74923828 74924451 74923828 ID=MS.gene68890.t1;Parent=MS.gene68890
chr4.3 exon 74923828 74924451 74923828 ID=MS.gene68890.t1.exon1;Parent=MS.gene68890.t1
chr4.3 CDS 74923828 74924451 74923828 ID=cds.MS.gene68890.t1;Parent=MS.gene68890.t1
Gene Sequence

>MS.gene68890

TCAAGAGATGATTACTTGGTGGTTTCATTGTTCTATAATGAAACCATACTTTATGCTGTTACAACACATTTGAAGTTTTTCTCATTTAGAGATAATACATGGAAAGAAATTCACTTCCCTTATGGTGTCATCTCTTCTGTCAGAAAAGGGTTGCTATTTAATGGGGTTATTCATTGGTTGGCTTTGCGTGAAGATTTAGGTTGGAATGTCATTGTTGCCTTTGATTTAATGGAAAGAAAGTTGTTTGAGATGCCTATTCCAAGTGATTCTGAACACAGAGCATTAAGACATACTGGTTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGGCTATGGATACTGCTAATGATACAATTGAAATATGGGTGATGAAAGAATACAATGTGCATTCATCTTGGACTAAAACTCTTGTTCTTTCTGTTGATACCATTCCTGAACCCTGCTTTCGCCCTATATGTTCTACAAAAAATGGTGATATTATTGGAATAAGTCCTGGGACTAGATTGGCGAAGTATAATGATAAAGGACAGTTATTAAGGCATCGCTCCTTTAATAGTCCATCTGATTATGTCATGTATACGGAGTCTCTGCTTTCACTCCCTGGTGACAATGAGCAAGTTTAA

Protein sequence

>MS.gene68890.t1

SRDDYLVVSLFYNETILYAVTTHLKFFSFRDNTWKEIHFPYGVISSVRKGLLFNGVIHWLALREDLGWNVIVAFDLMERKLFEMPIPSDSEHRALRHTGLWVFGEFLSLWAMDTANDTIEIWVMKEYNVHSSWTKTLVLSVDTIPEPCFRPICSTKNGDIIGISPGTRLAKYNDKGQLLRHRSFNSPSDYVMYTESLLSLPGDNEQV