AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene036556


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036556.t1 MTR_5g097210 68.702 131 40 1 5 134 227 357 1.43e-57 183
MS.gene036556.t1 MTR_1g055195 68.657 134 40 2 3 134 339 472 1.09e-55 181
MS.gene036556.t1 MTR_4g118370 59.701 134 54 0 1 134 121 254 1.83e-51 164
MS.gene036556.t1 MTR_1g055135 61.379 145 48 4 4 142 233 375 2.50e-50 164
MS.gene036556.t1 MTR_1g055175 60.563 142 51 4 4 142 62 201 5.87e-49 156
MS.gene036556.t1 MTR_1g055165 60.563 142 51 4 4 142 179 318 2.97e-47 155
MS.gene036556.t1 MTR_4g117720 55.172 145 57 3 5 142 235 378 4.28e-46 154
MS.gene036556.t1 MTR_4g109280 55.634 142 56 4 5 142 221 359 7.10e-45 150
MS.gene036556.t1 MTR_3g103020 55.634 142 57 3 5 142 239 378 1.01e-44 150
MS.gene036556.t1 MTR_1g055250 63.158 114 41 1 4 116 131 244 2.90e-44 147
MS.gene036556.t1 MTR_6g060650 51.799 139 63 2 5 141 236 372 6.00e-44 148
MS.gene036556.t1 MTR_4g117750 57.554 139 52 1 3 134 215 353 2.98e-41 143
MS.gene036556.t1 MTR_4g117750 53.211 109 47 3 34 138 373 481 7.93e-28 107
MS.gene036556.t1 MTR_8g040935 53.147 143 59 4 6 142 68 208 2.58e-40 134
MS.gene036556.t1 MTR_4g117870 56.250 128 52 3 16 141 141 266 4.24e-40 135
MS.gene036556.t1 MTR_3g034180 53.521 142 55 4 5 141 353 488 4.41e-40 140
MS.gene036556.t1 MTR_4g117850 53.571 140 57 4 5 139 240 376 6.02e-40 137
MS.gene036556.t1 MTR_4g109270 50.704 142 64 3 5 142 225 364 1.71e-38 134
MS.gene036556.t1 MTR_4g116330 51.389 144 62 4 5 142 239 380 1.82e-38 134
MS.gene036556.t1 MTR_4g023380 47.239 163 58 4 5 141 218 378 3.63e-38 133
MS.gene036556.t1 MTR_8g433030 55.970 134 53 4 5 134 205 336 5.05e-38 132
MS.gene036556.t1 MTR_4g116320 52.239 134 60 2 5 134 119 252 6.33e-38 129
MS.gene036556.t1 MTR_8g032390 55.970 134 53 4 5 134 238 369 1.32e-37 131
MS.gene036556.t1 MTR_4g118360 54.386 114 48 2 31 142 15 126 1.48e-37 124
MS.gene036556.t1 MTR_8g433040 54.286 140 57 4 5 141 228 363 1.72e-37 131
MS.gene036556.t1 MTR_8g032380 54.286 140 57 4 5 141 228 363 1.72e-37 131
MS.gene036556.t1 MTR_4g122850 49.655 145 69 4 1 141 221 365 6.72e-37 130
MS.gene036556.t1 MTR_4g094365 51.429 140 63 4 5 139 224 363 2.64e-36 128
MS.gene036556.t1 MTR_5g070080 52.113 142 62 4 5 142 247 386 5.08e-36 127
MS.gene036556.t1 MTR_4g118380 53.333 120 45 2 5 113 238 357 6.43e-35 124
MS.gene036556.t1 MTR_4g118400 53.030 132 48 4 5 134 175 294 6.71e-35 122
MS.gene036556.t1 MTR_5g070130 48.951 143 67 4 5 142 236 377 9.14e-35 124
MS.gene036556.t1 MTR_4g023090 47.097 155 62 6 5 141 233 385 1.15e-34 124
MS.gene036556.t1 MTR_3g034130 50.806 124 59 1 5 126 239 362 1.15e-34 124
MS.gene036556.t1 MTR_4g023070 49.032 155 58 5 5 142 220 370 1.92e-34 123
MS.gene036556.t1 MTR_6g463430 52.083 144 61 4 1 141 63 201 5.08e-34 118
MS.gene036556.t1 MTR_3g034160 51.079 139 63 3 5 141 239 374 1.60e-32 118
MS.gene036556.t1 MTR_5g070088 50.000 142 65 4 5 141 245 385 1.75e-32 118
MS.gene036556.t1 MTR_4g023120 48.701 154 58 6 5 141 220 369 5.89e-32 116
MS.gene036556.t1 MTR_4g021785 47.945 146 67 5 1 142 215 355 5.91e-32 116
MS.gene036556.t1 MTR_4g023080 51.773 141 59 5 5 141 218 353 8.58e-32 115
MS.gene036556.t1 MTR_4g023130 51.408 142 58 6 5 141 177 312 1.14e-31 114
MS.gene036556.t1 MTR_4g023190 51.613 155 49 9 1 140 214 357 1.67e-31 115
MS.gene036556.t1 MTR_4g023150 50.968 155 50 9 1 140 215 358 5.74e-31 114
MS.gene036556.t1 MTR_4g117770 52.083 144 60 5 5 141 225 366 7.46e-31 114
MS.gene036556.t1 MTR_4g117810 52.083 144 60 5 5 141 225 366 7.46e-31 114
MS.gene036556.t1 MTR_4g117780 51.389 144 61 5 5 141 225 366 8.37e-31 113
MS.gene036556.t1 MTR_4g023200 48.387 155 54 9 1 140 215 358 1.95e-28 107
MS.gene036556.t1 MTR_7g079400 44.681 141 71 2 5 138 195 335 2.13e-28 106
MS.gene036556.t1 MTR_5g023000 52.083 96 42 2 49 142 6 99 3.57e-27 97.4
MS.gene036556.t1 MTR_7g079370 47.015 134 67 3 5 135 219 351 1.98e-25 99.4
MS.gene036556.t1 MTR_4g116330 51.754 114 46 5 36 142 230 341 4.09e-25 98.2
MS.gene036556.t1 MTR_8g102520 41.912 136 76 2 2 134 181 316 7.04e-25 97.1
MS.gene036556.t1 MTR_7g079450 42.537 134 68 3 5 134 3 131 8.66e-25 92.8
MS.gene036556.t1 MTR_4g023440 51.579 95 40 3 49 141 241 331 9.59e-22 89.0
MS.gene036556.t1 MTR_4g117530 44.037 109 54 3 36 142 150 253 4.02e-21 85.9
MS.gene036556.t1 MTR_4g122860 42.623 122 59 4 21 134 196 314 6.67e-21 86.7
MS.gene036556.t1 MTR_7g079420 39.860 143 72 6 5 138 1005 1142 7.26e-20 85.1
MS.gene036556.t1 MTR_4g114700 43.269 104 51 3 36 131 115 218 2.30e-18 78.6
MS.gene036556.t1 MTR_4g121580 48.958 96 39 4 15 107 230 318 3.46e-18 79.7
MS.gene036556.t1 MTR_8g098830 43.443 122 62 4 5 123 89 206 3.63e-18 77.8
MS.gene036556.t1 MTR_2g048580 39.098 133 70 5 5 134 212 336 7.39e-16 72.8
MS.gene036556.t1 MTR_4g023430 45.783 83 38 3 64 141 141 221 1.87e-14 67.8
MS.gene036556.t1 MTR_7g117720 51.163 86 36 3 55 134 186 271 1.92e-14 68.6
MS.gene036556.t1 MTR_8g013005 50.000 60 28 1 82 141 1 58 2.68e-11 55.8
MS.gene036556.t1 MTR_4g122840 34.074 135 47 5 11 139 165 263 3.95e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTACACATTGTGGTTTA+TGG 0.100871 8.3:+8114165 MS.gene036556:CDS
GTAGTAACAAATGGTTCTTA+TGG 0.251967 8.3:+8114353 MS.gene036556:CDS
TTAGAGGATTTCTCAGTCTA+TGG 0.277856 8.3:+8114192 MS.gene036556:CDS
TTCTACACATTGTGGTTTAT+GGG 0.314260 8.3:+8114166 MS.gene036556:CDS
TATTGTTGCATTTCATTTAA+CGG 0.314412 8.3:+8114097 MS.gene036556:CDS
GATATGATGCAATTGATATA+TGG 0.320951 8.3:+8114222 MS.gene036556:CDS
AGATGCACTTGTATTTCTAT+TGG 0.341222 8.3:+8114430 MS.gene036556:CDS
TAGTAACAAATGGTTCTTAT+GGG 0.351884 8.3:+8114354 MS.gene036556:CDS
GAATCATAGTCAACATCAAC+TGG 0.373741 8.3:-8114146 None:intergenic
TGTGGTTTATGGGTATTTAG+AGG 0.378055 8.3:+8114176 MS.gene036556:CDS
TGCTCGTTGTCACCAGGAAC+AGG 0.396247 8.3:-8114482 None:intergenic
ATGAAGTCGGTGGGACCAAC+AGG 0.407414 8.3:-8114296 None:intergenic
CCACTCTTTGTACAACATAC+AGG 0.447432 8.3:-8114323 None:intergenic
AAGACTCTTGTTCTTCCTGT+TGG 0.456705 8.3:+8114281 MS.gene036556:CDS
TACAAGTGCATCTTTACAAA+AGG 0.470897 8.3:-8114420 None:intergenic
AATACAAAGTACATTCATCT+TGG 0.473647 8.3:+8114255 MS.gene036556:CDS
GGTGATATTGTAGTAACAAA+TGG 0.485444 8.3:+8114344 MS.gene036556:CDS
TAGAGGATTTCTCAGTCTAT+GGG 0.489737 8.3:+8114193 MS.gene036556:CDS
ATATGATGCAATTGATATAT+GGG 0.495926 8.3:+8114223 MS.gene036556:CDS
TTGCATTTCATTTAACGGAA+AGG 0.496118 8.3:+8114102 MS.gene036556:CDS
AACAAATGGTTCTTATGGGT+TGG 0.502071 8.3:+8114358 MS.gene036556:CDS
TAACCTTGCTCGTTGTCACC+AGG 0.523641 8.3:-8114488 None:intergenic
CACTCTTTGTACAACATACA+GGG 0.563133 8.3:-8114322 None:intergenic
TGCAATTGATATATGGGTGA+TGG 0.565148 8.3:+8114229 MS.gene036556:CDS
TAGTCAACATCAACTGGGAG+AGG 0.571901 8.3:-8114140 None:intergenic
GACTATGATTCTACACATTG+TGG 0.583006 8.3:+8114158 MS.gene036556:CDS
GTTCCTGGTGACAACGAGCA+AGG 0.617466 8.3:+8114485 MS.gene036556:CDS
CCTGTATGTTGTACAAAGAG+TGG 0.630075 8.3:+8114323 MS.gene036556:CDS
TACAGGGAAAATGAAGTCGG+TGG 0.632059 8.3:-8114306 None:intergenic
GCTCGTTGTCACCAGGAACA+GGG 0.633306 8.3:-8114481 None:intergenic
TTGGTGAAATATAACAACAA+AGG 0.638031 8.3:+8114377 MS.gene036556:CDS
ACAGGGAAAATGAAGTCGGT+GGG 0.646954 8.3:-8114305 None:intergenic
ACATACAGGGAAAATGAAGT+CGG 0.663561 8.3:-8114309 None:intergenic
AATCATAGTCAACATCAACT+GGG 0.686130 8.3:-8114145 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATGATGCAATTGATATAT+GGG + chr8.3:8114223-8114242 MS.gene036556:CDS 20.0%
!! TATTGTTGCATTTCATTTAA+CGG + chr8.3:8114097-8114116 MS.gene036556:CDS 20.0%
! AATACAAAGTACATTCATCT+TGG + chr8.3:8114255-8114274 MS.gene036556:CDS 25.0%
! GATATGATGCAATTGATATA+TGG + chr8.3:8114222-8114241 MS.gene036556:CDS 25.0%
! TAGTAACAAATGGTTCTTAT+GGG + chr8.3:8114354-8114373 MS.gene036556:CDS 25.0%
! TTGGTGAAATATAACAACAA+AGG + chr8.3:8114377-8114396 MS.gene036556:CDS 25.0%
AATCATAGTCAACATCAACT+GGG - chr8.3:8114148-8114167 None:intergenic 30.0%
ATTCTACACATTGTGGTTTA+TGG + chr8.3:8114165-8114184 MS.gene036556:CDS 30.0%
GGTGATATTGTAGTAACAAA+TGG + chr8.3:8114344-8114363 MS.gene036556:CDS 30.0%
GTAGTAACAAATGGTTCTTA+TGG + chr8.3:8114353-8114372 MS.gene036556:CDS 30.0%
TACAAGTGCATCTTTACAAA+AGG - chr8.3:8114423-8114442 None:intergenic 30.0%
TTCTACACATTGTGGTTTAT+GGG + chr8.3:8114166-8114185 MS.gene036556:CDS 30.0%
TTGCATTTCATTTAACGGAA+AGG + chr8.3:8114102-8114121 MS.gene036556:CDS 30.0%
! AGATGCACTTGTATTTCTAT+TGG + chr8.3:8114430-8114449 MS.gene036556:CDS 30.0%
AACAAATGGTTCTTATGGGT+TGG + chr8.3:8114358-8114377 MS.gene036556:CDS 35.0%
ACATACAGGGAAAATGAAGT+CGG - chr8.3:8114312-8114331 None:intergenic 35.0%
CACTCTTTGTACAACATACA+GGG - chr8.3:8114325-8114344 None:intergenic 35.0%
GAATCATAGTCAACATCAAC+TGG - chr8.3:8114149-8114168 None:intergenic 35.0%
GACTATGATTCTACACATTG+TGG + chr8.3:8114158-8114177 MS.gene036556:CDS 35.0%
TAGAGGATTTCTCAGTCTAT+GGG + chr8.3:8114193-8114212 MS.gene036556:CDS 35.0%
TGCAATTGATATATGGGTGA+TGG + chr8.3:8114229-8114248 MS.gene036556:CDS 35.0%
TGTGGTTTATGGGTATTTAG+AGG + chr8.3:8114176-8114195 MS.gene036556:CDS 35.0%
TTAGAGGATTTCTCAGTCTA+TGG + chr8.3:8114192-8114211 MS.gene036556:CDS 35.0%
AAGACTCTTGTTCTTCCTGT+TGG + chr8.3:8114281-8114300 MS.gene036556:CDS 40.0%
CCACTCTTTGTACAACATAC+AGG - chr8.3:8114326-8114345 None:intergenic 40.0%
CCTGTATGTTGTACAAAGAG+TGG + chr8.3:8114323-8114342 MS.gene036556:CDS 40.0%
ACAGGGAAAATGAAGTCGGT+GGG - chr8.3:8114308-8114327 None:intergenic 45.0%
TACAGGGAAAATGAAGTCGG+TGG - chr8.3:8114309-8114328 None:intergenic 45.0%
TAGTCAACATCAACTGGGAG+AGG - chr8.3:8114143-8114162 None:intergenic 45.0%
TAACCTTGCTCGTTGTCACC+AGG - chr8.3:8114491-8114510 None:intergenic 50.0%
!! CTGCTTTTACTCCCTGTTCC+TGG + chr8.3:8114470-8114489 MS.gene036556:CDS 50.0%
ATGAAGTCGGTGGGACCAAC+AGG - chr8.3:8114299-8114318 None:intergenic 55.0%
GCTCGTTGTCACCAGGAACA+GGG - chr8.3:8114484-8114503 None:intergenic 55.0%
GTTCCTGGTGACAACGAGCA+AGG + chr8.3:8114485-8114504 MS.gene036556:CDS 55.0%
TGCTCGTTGTCACCAGGAAC+AGG - chr8.3:8114485-8114504 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 8114083 8114511 8114083 ID=MS.gene036556
chr8.3 mRNA 8114083 8114511 8114083 ID=MS.gene036556.t1;Parent=MS.gene036556
chr8.3 exon 8114083 8114511 8114083 ID=MS.gene036556.t1.exon1;Parent=MS.gene036556.t1
chr8.3 CDS 8114083 8114511 8114083 ID=cds.MS.gene036556.t1;Parent=MS.gene036556.t1
Gene Sequence

>MS.gene036556

ATGGATGTCTTTGTTATTGTTGCATTTCATTTAACGGAAAGGAAACTTATAGAGATTCCTCTCCCAGTTGATGTTGACTATGATTCTACACATTGTGGTTTATGGGTATTTAGAGGATTTCTCAGTCTATGGGTTTTGAGATATGATGCAATTGATATATGGGTGATGGAAGAATACAAAGTACATTCATCTTGGACTAAGACTCTTGTTCTTCCTGTTGGTCCCACCGACTTCATTTTCCCTGTATGTTGTACAAAGAGTGGTGATATTGTAGTAACAAATGGTTCTTATGGGTTGGTGAAATATAACAACAAAGGAGAGTTTCTAGAGCGTAACTCCTTTTGTAAAGATGCACTTGTATTTCTATTGGCTATGTATACAGAGTCTCTGCTTTTACTCCCTGTTCCTGGTGACAACGAGCAAGGTTAA

Protein sequence

>MS.gene036556.t1

MDVFVIVAFHLTERKLIEIPLPVDVDYDSTHCGLWVFRGFLSLWVLRYDAIDIWVMEEYKVHSSWTKTLVLPVGPTDFIFPVCCTKSGDIVVTNGSYGLVKYNNKGEFLERNSFCKDALVFLLAMYTESLLLLPVPGDNEQG