AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90742


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90742.t1 MTR_1g023400 93.182 264 18 0 1 264 89 352 0.0 520
MS.gene90742.t1 MTR_1g022200 61.871 278 90 8 5 270 102 375 1.93e-106 313
MS.gene90742.t1 MTR_1g009110 46.209 277 129 9 1 265 111 379 1.75e-68 216
MS.gene90742.t1 MTR_4g080200 48.754 281 114 12 1 264 82 349 5.12e-68 214
MS.gene90742.t1 MTR_1g023430 69.014 142 38 2 132 270 40 178 1.03e-62 195
MS.gene90742.t1 MTR_1g037540 43.206 287 130 13 1 264 124 400 4.75e-55 182
MS.gene90742.t1 MTR_1g024125 73.214 112 29 1 137 247 38 149 2.26e-53 171
MS.gene90742.t1 MTR_1g022105 39.357 249 104 6 1 249 120 321 3.31e-49 165
MS.gene90742.t1 MTR_1g008000 35.231 281 112 15 3 265 41 269 1.23e-24 99.8
MS.gene90742.t1 MTR_0064s0160 25.784 287 173 13 2 263 148 419 5.67e-14 71.6
MS.gene90742.t1 MTR_2g021950 23.944 284 183 11 3 263 120 393 1.99e-11 63.9
MS.gene90742.t1 MTR_2g426090 25.939 293 176 14 1 264 96 376 3.06e-11 63.5
MS.gene90742.t1 MTR_1g061330 24.199 281 166 13 5 264 103 357 6.86e-11 62.4
MS.gene90742.t1 MTR_5g037680 26.293 232 144 10 5 226 126 340 8.73e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 77 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCCGTCATCTTGAGCATT+TGG 0.258740 1.4:+1337340 MS.gene90742:CDS
AATCAACATCAATTTGAAAT+TGG 0.285324 1.4:-1337518 None:intergenic
GCCCTCTGAGTTGGTGAAAC+TGG 0.301844 1.4:-1337224 None:intergenic
TGGTGTGTGGTCTTAGTAAA+AGG 0.318775 1.4:-1337087 None:intergenic
TAATTCCATCATAACTAGTA+TGG 0.350426 1.4:-1337002 None:intergenic
ATAGAATATTTGCAACAAAA+TGG 0.364437 1.4:-1337182 None:intergenic
GACTCATGCATGTATGGTTT+CGG 0.365854 1.4:+1336937 MS.gene90742:CDS
TCCCGTCATCTTGAGCATTT+GGG 0.381975 1.4:+1337341 MS.gene90742:CDS
TTGTTAACAGCGTTTATCAT+TGG 0.387639 1.4:+1337133 MS.gene90742:CDS
ATAATTTAAGTACCAACTCT+TGG 0.412072 1.4:+1337043 MS.gene90742:CDS
CATCTTGAGCATTTGGGTGA+TGG 0.434538 1.4:+1337347 MS.gene90742:CDS
GACAGCGACTCATGCATGTA+TGG 0.435390 1.4:+1336931 MS.gene90742:CDS
CGGTGGACCTCTCTAGTTGT+TGG 0.437420 1.4:-1336886 None:intergenic
CCTTGTCGACCTATCTAGCT+AGG 0.454046 1.4:+1337577 MS.gene90742:CDS
AACTTTGAAGTTGATGTTGT+TGG 0.457396 1.4:-1336971 None:intergenic
ATGATTATAGCAACTTTGCA+TGG 0.461665 1.4:+1337259 MS.gene90742:CDS
TCTGTCTCCAAGGTTTGTGT+TGG 0.467072 1.4:+1336824 MS.gene90742:CDS
AAACTCCATACTAGTTATGA+TGG 0.471735 1.4:+1336997 MS.gene90742:CDS
AAAGGAAGAGGATGTTCAGT+TGG 0.476611 1.4:-1337069 None:intergenic
GGTGGACCTCTCTAGTTGTT+GGG 0.479668 1.4:-1336885 None:intergenic
CAAATCCTTGGAGGAAAGGT+TGG 0.481459 1.4:+1337453 MS.gene90742:CDS
ACTTTGAAGTTGATGTTGTT+GGG 0.482647 1.4:-1336970 None:intergenic
CTATCTAGCTAGGGCACACT+AGG 0.484374 1.4:+1337587 MS.gene90742:CDS
AAGGAAGAGGATGTTCAGTT+GGG 0.484768 1.4:-1337068 None:intergenic
GCCGAGATCAAAGGTTCCCT+TGG 0.490016 1.4:+1337291 MS.gene90742:CDS
CTAGTGTGCCCTAGCTAGAT+AGG 0.491771 1.4:-1337586 None:intergenic
ACAAGAGTGCTATATCTAGA+AGG 0.495385 1.4:-1337114 None:intergenic
TGTTTCGTATGTGTGGCCTT+TGG 0.504996 1.4:+1337418 MS.gene90742:CDS
TATGAAACCAACACAAACCT+TGG 0.505418 1.4:-1336831 None:intergenic
GTTGATGTTGTTGGGATCAT+CGG 0.508372 1.4:-1336962 None:intergenic
GTCGACAAGGGAGCTATGCT+TGG 0.515430 1.4:-1337564 None:intergenic
ACCCAAATGCTCAAGATGAC+GGG 0.515580 1.4:-1337342 None:intergenic
AAGAATGGCGATCAAATCCT+TGG 0.518473 1.4:+1337441 MS.gene90742:CDS
GTTCTCCAACCTTTCCTCCA+AGG 0.525401 1.4:-1337458 None:intergenic
ATGTGTGGCCTTTGGAAGAA+TGG 0.525913 1.4:+1337426 MS.gene90742:CDS
TGATTATAGCAACTTTGCAT+GGG 0.536071 1.4:+1337260 MS.gene90742:CDS
TTCAGTTGGGGTCCAAGAGT+TGG 0.536792 1.4:-1337055 None:intergenic
ACCAAGGGAACCTTTGATCT+CGG 0.537068 1.4:-1337292 None:intergenic
CGATCAAATCCTTGGAGGAA+AGG 0.537340 1.4:+1337449 MS.gene90742:CDS
TTTGATCGCCATTCTTCCAA+AGG 0.545314 1.4:-1337434 None:intergenic
CACCCAAATGCTCAAGATGA+CGG 0.555873 1.4:-1337343 None:intergenic
CTCACATCATATGACTATCA+AGG 0.557817 1.4:+1337483 MS.gene90742:CDS
GATTGTGTTGCCGAGATCAA+AGG 0.560123 1.4:+1337282 MS.gene90742:CDS
TGGATTATAAGCAGTTCATA+CGG 0.560788 1.4:+1337153 MS.gene90742:CDS
TTTATAAGAATGGAATCATA+CGG 0.562387 1.4:+1336784 None:intergenic
GTGGTCTTAGTAAAAGGAAG+AGG 0.563530 1.4:-1337081 None:intergenic
GTCTTGATGAGTTGATTATG+TGG 0.567130 1.4:+1336860 MS.gene90742:CDS
GAATCATACGGTAGTTGCAA+TGG 0.573781 1.4:+1336796 MS.gene90742:CDS
TCGCTGTCAAGGCAGAGCCT+CGG 0.581840 1.4:-1336916 None:intergenic
TGCTATATCTAGAAGGACAT+TGG 0.583334 1.4:-1337107 None:intergenic
ACATGCATGAGTCGCTGTCA+AGG 0.587596 1.4:-1336927 None:intergenic
CTATAATCATATGAGAAGGT+TGG 0.594270 1.4:-1337246 None:intergenic
AGAAGGTTGGCCCTCTGAGT+TGG 0.594377 1.4:-1337233 None:intergenic
AATGGCGATCAAATCCTTGG+AGG 0.596817 1.4:+1337444 MS.gene90742:CDS
GGCAGAGCCTCGGAGGAACA+CGG 0.598335 1.4:-1336906 None:intergenic
CTTGTCGACCTATCTAGCTA+GGG 0.600363 1.4:+1337578 MS.gene90742:CDS
ACCAGTTTCACCAACTCAGA+GGG 0.601175 1.4:+1337223 MS.gene90742:CDS
TACGAAACATTGACACAAGT+GGG 0.601347 1.4:-1337404 None:intergenic
GTTGCTATAATCATATGAGA+AGG 0.609576 1.4:-1337250 None:intergenic
GTGTCAATGTTTCGTATGTG+TGG 0.615188 1.4:+1337411 MS.gene90742:CDS
TACAATGCACAACATAACCA+AGG 0.616377 1.4:-1337308 None:intergenic
CTGTCAAGGCAGAGCCTCGG+AGG 0.616821 1.4:-1336913 None:intergenic
ATACGAAACATTGACACAAG+TGG 0.622567 1.4:-1337405 None:intergenic
CCTAGCTAGATAGGTCGACA+AGG 0.638285 1.4:-1337577 None:intergenic
ACAATGCACAACATAACCAA+GGG 0.652154 1.4:-1337307 None:intergenic
AGGTCCACCGTGTTCCTCCG+AGG 0.662733 1.4:+1336899 MS.gene90742:CDS
AACCAGTTTCACCAACTCAG+AGG 0.670970 1.4:+1337222 MS.gene90742:CDS
GGGTGATGGATCAAAGTGGA+TGG 0.671155 1.4:+1337361 MS.gene90742:CDS
TGATGGAATTATGAGATCAG+CGG 0.673695 1.4:+1337014 MS.gene90742:CDS
GTGGAACCCAACAACTAGAG+AGG 0.673963 1.4:+1336879 MS.gene90742:CDS
AGGAAGAGGATGTTCAGTTG+GGG 0.674344 1.4:-1337067 None:intergenic
TGATAGTCATATGATGTGAG+CGG 0.685126 1.4:-1337480 None:intergenic
GTTGGTGAAACTGGTTGTTG+CGG 0.693105 1.4:-1337215 None:intergenic
TCTAGAAGGACATTGGTGTG+TGG 0.699232 1.4:-1337100 None:intergenic
CTAGCTAGATAGGTCGACAA+GGG 0.718320 1.4:-1337576 None:intergenic
ATTTGGGTGATGGATCAAAG+TGG 0.755177 1.4:+1337357 MS.gene90742:CDS
AGAGCCTCGGAGGAACACGG+TGG 0.797176 1.4:-1336903 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGAATATTTGCAACAAAA+TGG - chr1.4:1337185-1337204 None:intergenic 20.0%
!!! AATCAACATCAATTTGAAAT+TGG - chr1.4:1337521-1337540 None:intergenic 20.0%
! ATAATTTAAGTACCAACTCT+TGG + chr1.4:1337043-1337062 MS.gene90742:CDS 25.0%
! TAATTCCATCATAACTAGTA+TGG - chr1.4:1337005-1337024 None:intergenic 25.0%
AAACTCCATACTAGTTATGA+TGG + chr1.4:1336997-1337016 MS.gene90742:CDS 30.0%
ATGATTATAGCAACTTTGCA+TGG + chr1.4:1337259-1337278 MS.gene90742:CDS 30.0%
CTATAATCATATGAGAAGGT+TGG - chr1.4:1337249-1337268 None:intergenic 30.0%
GTTGCTATAATCATATGAGA+AGG - chr1.4:1337253-1337272 None:intergenic 30.0%
TGATTATAGCAACTTTGCAT+GGG + chr1.4:1337260-1337279 MS.gene90742:CDS 30.0%
TGGATTATAAGCAGTTCATA+CGG + chr1.4:1337153-1337172 MS.gene90742:CDS 30.0%
TTGTTAACAGCGTTTATCAT+TGG + chr1.4:1337133-1337152 MS.gene90742:CDS 30.0%
!! AACTTTGAAGTTGATGTTGT+TGG - chr1.4:1336974-1336993 None:intergenic 30.0%
!! ACTTTGAAGTTGATGTTGTT+GGG - chr1.4:1336973-1336992 None:intergenic 30.0%
ACAATGCACAACATAACCAA+GGG - chr1.4:1337310-1337329 None:intergenic 35.0%
ATACGAAACATTGACACAAG+TGG - chr1.4:1337408-1337427 None:intergenic 35.0%
CTCACATCATATGACTATCA+AGG + chr1.4:1337483-1337502 MS.gene90742:CDS 35.0%
TACAATGCACAACATAACCA+AGG - chr1.4:1337311-1337330 None:intergenic 35.0%
TACGAAACATTGACACAAGT+GGG - chr1.4:1337407-1337426 None:intergenic 35.0%
TATGAAACCAACACAAACCT+TGG - chr1.4:1336834-1336853 None:intergenic 35.0%
TGATAGTCATATGATGTGAG+CGG - chr1.4:1337483-1337502 None:intergenic 35.0%
TGATGGAATTATGAGATCAG+CGG + chr1.4:1337014-1337033 MS.gene90742:CDS 35.0%
TGCTATATCTAGAAGGACAT+TGG - chr1.4:1337110-1337129 None:intergenic 35.0%
!! ACAAGAGTGCTATATCTAGA+AGG - chr1.4:1337117-1337136 None:intergenic 35.0%
!! GTCTTGATGAGTTGATTATG+TGG + chr1.4:1336860-1336879 MS.gene90742:CDS 35.0%
AAAGGAAGAGGATGTTCAGT+TGG - chr1.4:1337072-1337091 None:intergenic 40.0%
AAGAATGGCGATCAAATCCT+TGG + chr1.4:1337441-1337460 MS.gene90742:CDS 40.0%
AAGGAAGAGGATGTTCAGTT+GGG - chr1.4:1337071-1337090 None:intergenic 40.0%
GAATCATACGGTAGTTGCAA+TGG + chr1.4:1336796-1336815 MS.gene90742:CDS 40.0%
GACTCATGCATGTATGGTTT+CGG + chr1.4:1336937-1336956 MS.gene90742:CDS 40.0%
GTGTCAATGTTTCGTATGTG+TGG + chr1.4:1337411-1337430 MS.gene90742:CDS 40.0%
GTTGATGTTGTTGGGATCAT+CGG - chr1.4:1336965-1336984 None:intergenic 40.0%
TTTGATCGCCATTCTTCCAA+AGG - chr1.4:1337437-1337456 None:intergenic 40.0%
! GTGGTCTTAGTAAAAGGAAG+AGG - chr1.4:1337084-1337103 None:intergenic 40.0%
! TGGCACAAAAAGTGCAACAT+TGG + chr1.4:1337381-1337400 MS.gene90742:CDS 40.0%
! TGGTGTGTGGTCTTAGTAAA+AGG - chr1.4:1337090-1337109 None:intergenic 40.0%
!! ATTTGGGTGATGGATCAAAG+TGG + chr1.4:1337357-1337376 MS.gene90742:CDS 40.0%
AACCAGTTTCACCAACTCAG+AGG + chr1.4:1337222-1337241 MS.gene90742:CDS 45.0%
AATGGCGATCAAATCCTTGG+AGG + chr1.4:1337444-1337463 MS.gene90742:CDS 45.0%
ACCAAGGGAACCTTTGATCT+CGG - chr1.4:1337295-1337314 None:intergenic 45.0%
ACCAGTTTCACCAACTCAGA+GGG + chr1.4:1337223-1337242 MS.gene90742:CDS 45.0%
ACCCAAATGCTCAAGATGAC+GGG - chr1.4:1337345-1337364 None:intergenic 45.0%
AGGAAGAGGATGTTCAGTTG+GGG - chr1.4:1337070-1337089 None:intergenic 45.0%
CAAATCCTTGGAGGAAAGGT+TGG + chr1.4:1337453-1337472 MS.gene90742:CDS 45.0%
CACCCAAATGCTCAAGATGA+CGG - chr1.4:1337346-1337365 None:intergenic 45.0%
CATCTTGAGCATTTGGGTGA+TGG + chr1.4:1337347-1337366 MS.gene90742:CDS 45.0%
CGATCAAATCCTTGGAGGAA+AGG + chr1.4:1337449-1337468 MS.gene90742:CDS 45.0%
CTAGCTAGATAGGTCGACAA+GGG - chr1.4:1337579-1337598 None:intergenic 45.0%
CTTGTCGACCTATCTAGCTA+GGG + chr1.4:1337578-1337597 MS.gene90742:CDS 45.0%
GATTGTGTTGCCGAGATCAA+AGG + chr1.4:1337282-1337301 MS.gene90742:CDS 45.0%
TCCCGTCATCTTGAGCATTT+GGG + chr1.4:1337341-1337360 MS.gene90742:CDS 45.0%
TCTAGAAGGACATTGGTGTG+TGG - chr1.4:1337103-1337122 None:intergenic 45.0%
TCTGTCTCCAAGGTTTGTGT+TGG + chr1.4:1336824-1336843 MS.gene90742:CDS 45.0%
TGTTTCGTATGTGTGGCCTT+TGG + chr1.4:1337418-1337437 MS.gene90742:CDS 45.0%
! AATGGCGTTTTCTGTCTCCA+AGG + chr1.4:1336814-1336833 MS.gene90742:CDS 45.0%
! ATGTGTGGCCTTTGGAAGAA+TGG + chr1.4:1337426-1337445 MS.gene90742:CDS 45.0%
! GTTGGTGAAACTGGTTGTTG+CGG - chr1.4:1337218-1337237 None:intergenic 45.0%
ACATGCATGAGTCGCTGTCA+AGG - chr1.4:1336930-1336949 None:intergenic 50.0%
CCTAGCTAGATAGGTCGACA+AGG - chr1.4:1337580-1337599 None:intergenic 50.0%
CCTTGTCGACCTATCTAGCT+AGG + chr1.4:1337577-1337596 MS.gene90742:CDS 50.0%
CTAGTGTGCCCTAGCTAGAT+AGG - chr1.4:1337589-1337608 None:intergenic 50.0%
CTCCCGTCATCTTGAGCATT+TGG + chr1.4:1337340-1337359 MS.gene90742:CDS 50.0%
GACAGCGACTCATGCATGTA+TGG + chr1.4:1336931-1336950 MS.gene90742:CDS 50.0%
GGTGGACCTCTCTAGTTGTT+GGG - chr1.4:1336888-1336907 None:intergenic 50.0%
GTGGAACCCAACAACTAGAG+AGG + chr1.4:1336879-1336898 MS.gene90742:CDS 50.0%
GTTCTCCAACCTTTCCTCCA+AGG - chr1.4:1337461-1337480 None:intergenic 50.0%
TTCAGTTGGGGTCCAAGAGT+TGG - chr1.4:1337058-1337077 None:intergenic 50.0%
!! GGGTGATGGATCAAAGTGGA+TGG + chr1.4:1337361-1337380 MS.gene90742:CDS 50.0%
CGGTGGACCTCTCTAGTTGT+TGG - chr1.4:1336889-1336908 None:intergenic 55.0%
GCCGAGATCAAAGGTTCCCT+TGG + chr1.4:1337291-1337310 MS.gene90742:CDS 55.0%
GTCGACAAGGGAGCTATGCT+TGG - chr1.4:1337567-1337586 None:intergenic 55.0%
! AGAAGGTTGGCCCTCTGAGT+TGG - chr1.4:1337236-1337255 None:intergenic 55.0%
!! GCCCTCTGAGTTGGTGAAAC+TGG - chr1.4:1337227-1337246 None:intergenic 55.0%
TCGCTGTCAAGGCAGAGCCT+CGG - chr1.4:1336919-1336938 None:intergenic 60.0%
AGAGCCTCGGAGGAACACGG+TGG - chr1.4:1336906-1336925 None:intergenic 65.0%
AGGTCCACCGTGTTCCTCCG+AGG + chr1.4:1336899-1336918 MS.gene90742:CDS 65.0%
CTGTCAAGGCAGAGCCTCGG+AGG - chr1.4:1336916-1336935 None:intergenic 65.0%
GGCAGAGCCTCGGAGGAACA+CGG - chr1.4:1336909-1336928 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 1336793 1337608 1336793 ID=MS.gene90742
chr1.4 mRNA 1336793 1337608 1336793 ID=MS.gene90742.t1;Parent=MS.gene90742
chr1.4 exon 1336793 1337608 1336793 ID=MS.gene90742.t1.exon1;Parent=MS.gene90742.t1
chr1.4 CDS 1336793 1337608 1336793 ID=cds.MS.gene90742.t1;Parent=MS.gene90742.t1
Gene Sequence

>MS.gene90742

ATGGAATCATACGGTAGTTGCAATGGCGTTTTCTGTCTCCAAGGTTTGTGTTGGTTTCATAAGAGTTGTCTTGATGAGTTGATTATGTGGAACCCAACAACTAGAGAGGTCCACCGTGTTCCTCCGAGGCTCTGCCTTGACAGCGACTCATGCATGTATGGTTTCGGTGCCGATGATCCCAACAACATCAACTTCAAAGTTGTGAAACTCCATACTAGTTATGATGGAATTATGAGATCAGCGGAAGTTTATAATTTAAGTACCAACTCTTGGACCCCAACTGAACATCCTCTTCCTTTTACTAAGACCACACACCAATGTCCTTCTAGATATAGCACTCTTGTTAACAGCGTTTATCATTGGATTATAAGCAGTTCATACGGCAATCGCCATTTTGTTGCAAATATTCTATGCTTTGACTTCCGCAACAACCAGTTTCACCAACTCAGAGGGCCAACCTTCTCATATGATTATAGCAACTTTGCATGGGATTGTGTTGCCGAGATCAAAGGTTCCCTTGGTTATGTTGTGCATTGTAACTTCAATGCTCCCGTCATCTTGAGCATTTGGGTGATGGATCAAAGTGGATGGCACAAAAAGTGCAACATTGGCCCACTTGTGTCAATGTTTCGTATGTGTGGCCTTTGGAAGAATGGCGATCAAATCCTTGGAGGAAAGGTTGGAGAACCGCTCACATCATATGACTATCAAGGTAACTCACTTGACCAATTTCAAATTGATGTTGATTATATTAAACTTCATGAGTATGTGCCAAGCATAGCTCCCTTGTCGACCTATCTAGCTAGGGCACACTAG

Protein sequence

>MS.gene90742.t1

MESYGSCNGVFCLQGLCWFHKSCLDELIMWNPTTREVHRVPPRLCLDSDSCMYGFGADDPNNINFKVVKLHTSYDGIMRSAEVYNLSTNSWTPTEHPLPFTKTTHQCPSRYSTLVNSVYHWIISSSYGNRHFVANILCFDFRNNQFHQLRGPTFSYDYSNFAWDCVAEIKGSLGYVVHCNFNAPVILSIWVMDQSGWHKKCNIGPLVSMFRMCGLWKNGDQILGGKVGEPLTSYDYQGNSLDQFQIDVDYIKLHEYVPSIAPLSTYLARAH