AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54049

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54049.t1 MTR_1g009110 94.921 315 13 1 1 315 68 379 0.0 626
MS.gene54049.t1 MTR_1g022105 58.108 296 72 7 1 294 75 320 3.38e-105 310
MS.gene54049.t1 MTR_1g023400 45.513 312 140 11 13 314 61 352 5.61e-75 234
MS.gene54049.t1 MTR_4g080200 43.689 309 155 11 13 314 53 349 5.46e-66 211
MS.gene54049.t1 MTR_1g022200 44.969 318 144 13 11 315 71 370 1.13e-64 208
MS.gene54049.t1 MTR_1g037540 41.554 296 148 12 36 314 113 400 1.23e-55 186
MS.gene54049.t1 MTR_1g023430 51.493 134 64 1 182 315 41 173 6.14e-39 135
MS.gene54049.t1 MTR_1g024125 52.727 110 48 2 186 291 38 147 1.18e-31 116
MS.gene54049.t1 MTR_1g008000 32.982 285 121 17 47 315 39 269 2.81e-19 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 92 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTACTATAGATGTTTA+TGG 0.171813 1.2:-10549479 MS.gene54049:CDS
TGGCCCAATGTTGTACATTT+TGG 0.236356 1.2:+10549632 None:intergenic
GGGCTCCCTTGCTTATGTTT+TGG 0.242082 1.2:-10549715 MS.gene54049:CDS
CATGTTGCATAAGTGATTTC+TGG 0.246825 1.2:-10549599 MS.gene54049:CDS
TACTTCTGCTCCCTCTTTCC+TGG 0.291167 1.2:-10550266 MS.gene54049:CDS
CGAACTGGGATGCGGTTAAA+TGG 0.329209 1.2:+10549912 None:intergenic
GAATTACTATAGATGTTTAT+GGG 0.331663 1.2:-10549478 MS.gene54049:CDS
GTCCGATCCAGTTGGAAATC+TGG 0.346576 1.2:-10549677 MS.gene54049:CDS
AAATATGATGATTCCCTGTA+TGG 0.359503 1.2:-10550083 MS.gene54049:CDS
GGTGGACCTGTTTAGTTGTT+GGG 0.363221 1.2:+10550138 None:intergenic
AAGGATGGCGATCAAGTCTT+TGG 0.371759 1.2:-10549576 MS.gene54049:CDS
ATTTATCACAGACCCATTGA+TGG 0.376040 1.2:-10550359 MS.gene54049:CDS
TTGTTAACTGTGTTTATCAT+TGG 0.385806 1.2:-10549866 MS.gene54049:CDS
TATGATGGATTGTATCGAAC+TGG 0.389997 1.2:+10549897 None:intergenic
CCAGTTGGAAATCTGGATTA+TGG 0.392026 1.2:-10549670 MS.gene54049:CDS
CTCTTGGTCAATTGATCCTA+TGG 0.400602 1.2:-10550163 MS.gene54049:CDS
ATATGTTTCGATTCGGTCTA+CGG 0.400923 1.2:+10550235 None:intergenic
ATGCAACATGTCCACGGAAC+TGG 0.401019 1.2:+10549612 None:intergenic
AATGGTGGAGGGTGATGGAC+AGG 0.404814 1.2:+10549930 None:intergenic
CGGTCTACGGATTGATTACC+AGG 0.408180 1.2:+10550248 None:intergenic
AGGTGGACCTGTTTAGTTGT+TGG 0.412563 1.2:+10550137 None:intergenic
GAGAAGATGATTATTCCTTC+AGG 0.423139 1.2:-10549990 MS.gene54049:CDS
TGATAAACACAGTTAACAAC+CGG 0.423981 1.2:+10549870 None:intergenic
CTCTCCTGTGACGGTGGCGA+CGG 0.438612 1.2:-10550311 MS.gene54049:CDS
CAAGTCTTTGGAGGAAAGGT+TGG 0.443153 1.2:-10549564 MS.gene54049:CDS
GTTTAGTTGTTGGGTTCCAT+AGG 0.448822 1.2:+10550147 None:intergenic
ATGGAACCCAACAACTAAAC+AGG 0.452752 1.2:-10550144 MS.gene54049:CDS
CGAAAACATTGCTGAGATCA+AGG 0.453867 1.2:-10549736 MS.gene54049:CDS
AATTGCCATATGTTTCGATT+CGG 0.459622 1.2:+10550228 None:intergenic
GCCTCCGTCGCCACCGTCAC+AGG 0.460383 1.2:+10550307 None:intergenic
ACAGGAGAGAAGAGTGATGT+AGG 0.461686 1.2:+10550325 None:intergenic
AGAATGTTTAACGTGCCGAC+AGG 0.462860 1.2:+10549761 None:intergenic
GGACCAAAATGTACAACATT+GGG 0.464466 1.2:-10549635 MS.gene54049:CDS
ATTTCCAACTGGATCGGACT+AGG 0.486938 1.2:+10549681 None:intergenic
TCCTGTGACGGTGGCGACGG+AGG 0.500634 1.2:-10550308 MS.gene54049:CDS
GGGAGGTTTGAATCCATCAA+TGG 0.500733 1.2:+10550346 None:intergenic
TGGCGACGGAGGCGAGGAGT+TGG 0.501551 1.2:-10550297 MS.gene54049:CDS
CCATAATCCAGATTTCCAAC+TGG 0.510407 1.2:+10549670 None:intergenic
ATGATCTAAGCACTAAATCA+TGG 0.514952 1.2:-10549956 MS.gene54049:CDS
GAGTGACAGAATGAGGGTGC+AGG 0.515232 1.2:+10550107 None:intergenic
AGGAAAGGTTGGAAAGCTGC+TGG 0.518994 1.2:-10549553 MS.gene54049:CDS
CAGGAGAGAAGAGTGATGTA+GGG 0.521709 1.2:+10550326 None:intergenic
GTAGACCGAATCGAAACATA+TGG 0.532232 1.2:-10550233 MS.gene54049:CDS
CGGATTGATTACCAGGAAAG+AGG 0.534555 1.2:+10550255 None:intergenic
CGATCAAGTCTTTGGAGGAA+AGG 0.536754 1.2:-10549568 MS.gene54049:CDS
TCTGGATTATGGATCAGAAC+CGG 0.536974 1.2:-10549659 MS.gene54049:CDS
TAAATGCACAGAAACCATAC+AGG 0.538550 1.2:+10550069 None:intergenic
ATATTTAGAGTGACAGAATG+AGG 0.539503 1.2:+10550100 None:intergenic
TGGACCAAAATGTACAACAT+TGG 0.539844 1.2:-10549636 MS.gene54049:CDS
CTGGCTTCATATGACGATCA+AGG 0.540639 1.2:-10549534 MS.gene54049:CDS
GGTTAAATGGTGGAGGGTGA+TGG 0.542091 1.2:+10549925 None:intergenic
TGAGGTGAGCTAGGTTGACA+AGG 0.543773 1.2:+10549428 None:intergenic
TTAACAACCGGGGTATATGA+TGG 0.545027 1.2:+10549882 None:intergenic
TGACGGTGGCGACGGAGGCG+AGG 0.546343 1.2:-10550303 MS.gene54049:CDS
GGAGGTTTGAATCCATCAAT+GGG 0.551734 1.2:+10550347 None:intergenic
ACTCTTCTCTCCTGTGACGG+TGG 0.556337 1.2:-10550317 MS.gene54049:CDS
AATTACTATAGATGTTTATG+GGG 0.557410 1.2:-10549477 MS.gene54049:CDS
GAGAGAAGAGTGATGTAGGG+AGG 0.559362 1.2:+10550329 None:intergenic
ATCACTCTTCTCTCCTGTGA+CGG 0.564386 1.2:-10550320 MS.gene54049:CDS
GATGGCGATCAAGTCTTTGG+AGG 0.568823 1.2:-10549573 MS.gene54049:CDS
GAAAACATTGCTGAGATCAA+GGG 0.570059 1.2:-10549735 MS.gene54049:CDS
ATCCAGATTTCCAACTGGAT+CGG 0.573766 1.2:+10549675 None:intergenic
GATACTCCAAAACATAAGCA+AGG 0.575880 1.2:+10549709 None:intergenic
AGTGACAGAATGAGGGTGCA+GGG 0.576394 1.2:+10550108 None:intergenic
ATAAGTGATTTCTGGAAGGA+TGG 0.580005 1.2:-10549591 MS.gene54049:CDS
GCCGACAGGGAGCTTTGAGT+TGG 0.583956 1.2:+10549775 None:intergenic
GATTGTATCGAACTGGGATG+CGG 0.586322 1.2:+10549904 None:intergenic
GAAACATATGGCAATTGCAA+TGG 0.588891 1.2:-10550221 MS.gene54049:CDS
TCATACACTTCTGCGCCTGA+AGG 0.591235 1.2:+10549975 None:intergenic
GGGATGCGGTTAAATGGTGG+AGG 0.595710 1.2:+10549918 None:intergenic
GGATTGATTACCAGGAAAGA+GGG 0.601607 1.2:+10550256 None:intergenic
ATGGTGGAGGGTGATGGACA+GGG 0.604225 1.2:+10549931 None:intergenic
TCATCCTAGTCCGATCCAGT+TGG 0.605888 1.2:-10549685 MS.gene54049:CDS
ACAACATTGGGCCAGTTCCG+TGG 0.611189 1.2:-10549623 MS.gene54049:CDS
ACTGGGATGCGGTTAAATGG+TGG 0.612497 1.2:+10549915 None:intergenic
TGGTGGAGGGTGATGGACAG+GGG 0.617953 1.2:+10549932 None:intergenic
TCACTTATGCAACATGTCCA+CGG 0.629494 1.2:+10549606 None:intergenic
GAATGTTTAACGTGCCGACA+GGG 0.630721 1.2:+10549762 None:intergenic
TATTTAGAGTGACAGAATGA+GGG 0.632064 1.2:+10550101 None:intergenic
GAGGTGAGCTAGGTTGACAA+GGG 0.632872 1.2:+10549429 None:intergenic
TGACAGAATGAGGGTGCAGG+GGG 0.633633 1.2:+10550110 None:intergenic
ATACTCCAAAACATAAGCAA+GGG 0.638978 1.2:+10549710 None:intergenic
GTGACAGAATGAGGGTGCAG+GGG 0.639675 1.2:+10550109 None:intergenic
GATAAACACAGTTAACAACC+GGG 0.639807 1.2:+10549871 None:intergenic
GGATTATGGATCAGAACCGG+TGG 0.658826 1.2:-10549656 MS.gene54049:CDS
TTGCATAAGTGATTTCTGGA+AGG 0.673241 1.2:-10549595 MS.gene54049:CDS
ATACAATCCATCATATACCC+CGG 0.677642 1.2:-10549889 MS.gene54049:CDS
ATGATGGATTGTATCGAACT+GGG 0.683119 1.2:+10549898 None:intergenic
GGATGCGGTTAAATGGTGGA+GGG 0.686361 1.2:+10549919 None:intergenic
ACCAACTCAAAGCTCCCTGT+CGG 0.686762 1.2:-10549776 MS.gene54049:CDS
AAATGCACAGAAACCATACA+GGG 0.706345 1.2:+10550070 None:intergenic
ATAAACACAGTTAACAACCG+GGG 0.720377 1.2:+10549872 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTACTATAGATGTTTATG+GGG - chr1.2:10550322-10550341 MS.gene54049:CDS 20.0%
!! GAATTACTATAGATGTTTAT+GGG - chr1.2:10550321-10550340 MS.gene54049:CDS 20.0%
!! TGAATTACTATAGATGTTTA+TGG - chr1.2:10550320-10550339 MS.gene54049:CDS 20.0%
!!! TCTTCTTAAAAGCTTTTTAT+AGG - chr1.2:10549606-10549625 MS.gene54049:CDS 20.0%
! TTGTTAACTGTGTTTATCAT+TGG - chr1.2:10549933-10549952 MS.gene54049:CDS 25.0%
!! TTTTATAGGAATTCAACTCT+TGG - chr1.2:10549620-10549639 MS.gene54049:CDS 25.0%
AAATATGATGATTCCCTGTA+TGG - chr1.2:10549716-10549735 MS.gene54049:CDS 30.0%
AATTGCCATATGTTTCGATT+CGG + chr1.2:10549574-10549593 None:intergenic 30.0%
ATACTCCAAAACATAAGCAA+GGG + chr1.2:10550092-10550111 None:intergenic 30.0%
ATGATCTAAGCACTAAATCA+TGG - chr1.2:10549843-10549862 MS.gene54049:CDS 30.0%
TGATAAACACAGTTAACAAC+CGG + chr1.2:10549932-10549951 None:intergenic 30.0%
! ATATTTAGAGTGACAGAATG+AGG + chr1.2:10549702-10549721 None:intergenic 30.0%
! TATTTAGAGTGACAGAATGA+GGG + chr1.2:10549701-10549720 None:intergenic 30.0%
AAATGCACAGAAACCATACA+GGG + chr1.2:10549732-10549751 None:intergenic 35.0%
ATAAACACAGTTAACAACCG+GGG + chr1.2:10549930-10549949 None:intergenic 35.0%
ATACAATCCATCATATACCC+CGG - chr1.2:10549910-10549929 MS.gene54049:CDS 35.0%
ATTTATCACAGACCCATTGA+TGG - chr1.2:10549440-10549459 MS.gene54049:CDS 35.0%
CATGTTGCATAAGTGATTTC+TGG - chr1.2:10550200-10550219 MS.gene54049:CDS 35.0%
GAAAACATTGCTGAGATCAA+GGG - chr1.2:10550064-10550083 MS.gene54049:CDS 35.0%
GAAACATATGGCAATTGCAA+TGG - chr1.2:10549578-10549597 MS.gene54049:CDS 35.0%
GAGAAGATGATTATTCCTTC+AGG - chr1.2:10549809-10549828 MS.gene54049:CDS 35.0%
GATAAACACAGTTAACAACC+GGG + chr1.2:10549931-10549950 None:intergenic 35.0%
GATACTCCAAAACATAAGCA+AGG + chr1.2:10550093-10550112 None:intergenic 35.0%
GGACCAAAATGTACAACATT+GGG - chr1.2:10550164-10550183 MS.gene54049:CDS 35.0%
TAAATGCACAGAAACCATAC+AGG + chr1.2:10549733-10549752 None:intergenic 35.0%
TGGACCAAAATGTACAACAT+TGG - chr1.2:10550163-10550182 MS.gene54049:CDS 35.0%
! ATAAGTGATTTCTGGAAGGA+TGG - chr1.2:10550208-10550227 MS.gene54049:CDS 35.0%
! TTGCATAAGTGATTTCTGGA+AGG - chr1.2:10550204-10550223 MS.gene54049:CDS 35.0%
!! ATATGTTTCGATTCGGTCTA+CGG + chr1.2:10549567-10549586 None:intergenic 35.0%
!! ATGATGGATTGTATCGAACT+GGG + chr1.2:10549904-10549923 None:intergenic 35.0%
!! TATGATGGATTGTATCGAAC+TGG + chr1.2:10549905-10549924 None:intergenic 35.0%
ATCCAGATTTCCAACTGGAT+CGG + chr1.2:10550127-10550146 None:intergenic 40.0%
ATGGAACCCAACAACTAAAC+AGG - chr1.2:10549655-10549674 MS.gene54049:CDS 40.0%
CCAGTTGGAAATCTGGATTA+TGG - chr1.2:10550129-10550148 MS.gene54049:CDS 40.0%
CCATAATCCAGATTTCCAAC+TGG + chr1.2:10550132-10550151 None:intergenic 40.0%
CGAAAACATTGCTGAGATCA+AGG - chr1.2:10550063-10550082 MS.gene54049:CDS 40.0%
CTCTTGGTCAATTGATCCTA+TGG - chr1.2:10549636-10549655 MS.gene54049:CDS 40.0%
GGAGGTTTGAATCCATCAAT+GGG + chr1.2:10549455-10549474 None:intergenic 40.0%
GGATTGATTACCAGGAAAGA+GGG + chr1.2:10549546-10549565 None:intergenic 40.0%
GTAGACCGAATCGAAACATA+TGG - chr1.2:10549566-10549585 MS.gene54049:CDS 40.0%
GTTTAGTTGTTGGGTTCCAT+AGG + chr1.2:10549655-10549674 None:intergenic 40.0%
TCACTTATGCAACATGTCCA+CGG + chr1.2:10550196-10550215 None:intergenic 40.0%
TCTGGATTATGGATCAGAAC+CGG - chr1.2:10550140-10550159 MS.gene54049:CDS 40.0%
TTAACAACCGGGGTATATGA+TGG + chr1.2:10549920-10549939 None:intergenic 40.0%
! TGGCCCAATGTTGTACATTT+TGG + chr1.2:10550170-10550189 None:intergenic 40.0%
!! TGTTGTACATTTTGGTCCAC+CGG + chr1.2:10550162-10550181 None:intergenic 40.0%
AAGGATGGCGATCAAGTCTT+TGG - chr1.2:10550223-10550242 MS.gene54049:CDS 45.0%
ACAGGAGAGAAGAGTGATGT+AGG + chr1.2:10549477-10549496 None:intergenic 45.0%
AGGTGGACCTGTTTAGTTGT+TGG + chr1.2:10549665-10549684 None:intergenic 45.0%
ATCACTCTTCTCTCCTGTGA+CGG - chr1.2:10549479-10549498 MS.gene54049:CDS 45.0%
ATTTCCAACTGGATCGGACT+AGG + chr1.2:10550121-10550140 None:intergenic 45.0%
CAGGAGAGAAGAGTGATGTA+GGG + chr1.2:10549476-10549495 None:intergenic 45.0%
CGGATTGATTACCAGGAAAG+AGG + chr1.2:10549547-10549566 None:intergenic 45.0%
CTGGCTTCATATGACGATCA+AGG - chr1.2:10550265-10550284 MS.gene54049:CDS 45.0%
GATTGTATCGAACTGGGATG+CGG + chr1.2:10549898-10549917 None:intergenic 45.0%
GGGAGGTTTGAATCCATCAA+TGG + chr1.2:10549456-10549475 None:intergenic 45.0%
GGTGGACCTGTTTAGTTGTT+GGG + chr1.2:10549664-10549683 None:intergenic 45.0%
! AGAATGTTTAACGTGCCGAC+AGG + chr1.2:10550041-10550060 None:intergenic 45.0%
! CAAGTCTTTGGAGGAAAGGT+TGG - chr1.2:10550235-10550254 MS.gene54049:CDS 45.0%
! CGATCAAGTCTTTGGAGGAA+AGG - chr1.2:10550231-10550250 MS.gene54049:CDS 45.0%
! GAATGTTTAACGTGCCGACA+GGG + chr1.2:10550040-10550059 None:intergenic 45.0%
ACCAACTCAAAGCTCCCTGT+CGG - chr1.2:10550023-10550042 MS.gene54049:CDS 50.0%
ACTGGGATGCGGTTAAATGG+TGG + chr1.2:10549887-10549906 None:intergenic 50.0%
AGGAAAGGTTGGAAAGCTGC+TGG - chr1.2:10550246-10550265 MS.gene54049:CDS 50.0%
ATGCAACATGTCCACGGAAC+TGG + chr1.2:10550190-10550209 None:intergenic 50.0%
CGAACTGGGATGCGGTTAAA+TGG + chr1.2:10549890-10549909 None:intergenic 50.0%
GAGAGAAGAGTGATGTAGGG+AGG + chr1.2:10549473-10549492 None:intergenic 50.0%
GATGGCGATCAAGTCTTTGG+AGG - chr1.2:10550226-10550245 MS.gene54049:CDS 50.0%
GGATTATGGATCAGAACCGG+TGG - chr1.2:10550143-10550162 MS.gene54049:CDS 50.0%
GTCCGATCCAGTTGGAAATC+TGG - chr1.2:10550122-10550141 MS.gene54049:CDS 50.0%
TACTTCTGCTCCCTCTTTCC+TGG - chr1.2:10549533-10549552 MS.gene54049:CDS 50.0%
TCATACACTTCTGCGCCTGA+AGG + chr1.2:10549827-10549846 None:intergenic 50.0%
TCATCCTAGTCCGATCCAGT+TGG - chr1.2:10550114-10550133 MS.gene54049:CDS 50.0%
! AGTGACAGAATGAGGGTGCA+GGG + chr1.2:10549694-10549713 None:intergenic 50.0%
! CGGTCTACGGATTGATTACC+AGG + chr1.2:10549554-10549573 None:intergenic 50.0%
! GGATGCGGTTAAATGGTGGA+GGG + chr1.2:10549883-10549902 None:intergenic 50.0%
! GGGCTCCCTTGCTTATGTTT+TGG - chr1.2:10550084-10550103 MS.gene54049:CDS 50.0%
! GGTTAAATGGTGGAGGGTGA+TGG + chr1.2:10549877-10549896 None:intergenic 50.0%
ACAACATTGGGCCAGTTCCG+TGG - chr1.2:10550176-10550195 MS.gene54049:CDS 55.0%
ACTCTTCTCTCCTGTGACGG+TGG - chr1.2:10549482-10549501 MS.gene54049:CDS 55.0%
GGGATGCGGTTAAATGGTGG+AGG + chr1.2:10549884-10549903 None:intergenic 55.0%
! ATGAGGGTGCAGGGGGTATA+AGG + chr1.2:10549685-10549704 None:intergenic 55.0%
! GAGTGACAGAATGAGGGTGC+AGG + chr1.2:10549695-10549714 None:intergenic 55.0%
! GTGACAGAATGAGGGTGCAG+GGG + chr1.2:10549693-10549712 None:intergenic 55.0%
! TGACAGAATGAGGGTGCAGG+GGG + chr1.2:10549692-10549711 None:intergenic 55.0%
!! AATGGTGGAGGGTGATGGAC+AGG + chr1.2:10549872-10549891 None:intergenic 55.0%
!! ATGGTGGAGGGTGATGGACA+GGG + chr1.2:10549871-10549890 None:intergenic 55.0%
! AGGGTGCAGGGGGTATAAGG+TGG + chr1.2:10549682-10549701 None:intergenic 60.0%
!! GCCGACAGGGAGCTTTGAGT+TGG + chr1.2:10550027-10550046 None:intergenic 60.0%
!! TGGTGGAGGGTGATGGACAG+GGG + chr1.2:10549870-10549889 None:intergenic 60.0%
CTCTCCTGTGACGGTGGCGA+CGG - chr1.2:10549488-10549507 MS.gene54049:CDS 65.0%
TCCTGTGACGGTGGCGACGG+AGG - chr1.2:10549491-10549510 MS.gene54049:CDS 70.0%
TGGCGACGGAGGCGAGGAGT+TGG - chr1.2:10549502-10549521 MS.gene54049:CDS 70.0%
GCCTCCGTCGCCACCGTCAC+AGG + chr1.2:10549495-10549514 None:intergenic 75.0%
TGACGGTGGCGACGGAGGCG+AGG - chr1.2:10549496-10549515 MS.gene54049:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 10549437 10550384 10549437 ID=MS.gene54049
chr1.2 mRNA 10549437 10550384 10549437 ID=MS.gene54049.t1;Parent=MS.gene54049
chr1.2 exon 10549437 10550384 10549437 ID=MS.gene54049.t1.exon1;Parent=MS.gene54049.t1
chr1.2 CDS 10549437 10550384 10549437 ID=cds.MS.gene54049.t1;Parent=MS.gene54049.t1
Gene Sequence

>MS.gene54049

ATGATTTATCACAGACCCATTGATGGATTCAAACCTCCCTACATCACTCTTCTCTCCTGTGACGGTGGCGACGGAGGCGAGGAGTTGGAACGTATCTACTTCTGCTCCCTCTTTCCTGGTAATCAATCCGTAGACCGAATCGAAACATATGGCAATTGCAATGGTGTTTTCTTCTTAAAAGCTTTTTATAGGAATTCAACTCTTGGTCAATTGATCCTATGGAACCCAACAACTAAACAGGTCCACCTTATACCCCCTGCACCCTCATTCTGTCACTCTAAATATGATGATTCCCTGTATGGTTTCTGTGCATTTAACGACGACTGTAACATTAATTTCAAAGTTGTTAGACTTCAACAATGTGCACATGTTGAGAAGATGATTATTCCTTCAGGCGCAGAAGTGTATGATCTAAGCACTAAATCATGGACCCCTGTCCATCACCCTCCACCATTTAACCGCATCCCAGTTCGATACAATCCATCATATACCCCGGTTGTTAACTGTGTTTATCATTGGATTGTTACTGTCGATTTGCATACTACTTCAAACATCATCTGCTTTGACTTTCACAACAATCAGTTTCACCAACTCAAAGCTCCCTGTCGGCACGTTAAACATTCTAGCGAAAACATTGCTGAGATCAAGGGCTCCCTTGCTTATGTTTTGGAGTATCATCATCCTAGTCCGATCCAGTTGGAAATCTGGATTATGGATCAGAACCGGTGGACCAAAATGTACAACATTGGGCCAGTTCCGTGGACATGTTGCATAAGTGATTTCTGGAAGGATGGCGATCAAGTCTTTGGAGGAAAGGTTGGAAAGCTGCTGGCTTCATATGACGATCAAGGCAACTCGCTCAGCGAATTTCAAACTTATGTTTTGAATTACTATAGATGTTTATGGGGTTCTGAGTATTTACAGAGCATAACTCCCTTGTCAACCTAG
Protein sequence

>MS.gene54049.t1

MIYHRPIDGFKPPYITLLSCDGGDGGEELERIYFCSLFPGNQSVDRIETYGNCNGVFFLKAFYRNSTLGQLILWNPTTKQVHLIPPAPSFCHSKYDDSLYGFCAFNDDCNINFKVVRLQQCAHVEKMIIPSGAEVYDLSTKSWTPVHHPPPFNRIPVRYNPSYTPVVNCVYHWIVTVDLHTTSNIICFDFHNNQFHQLKAPCRHVKHSSENIAEIKGSLAYVLEYHHPSPIQLEIWIMDQNRWTKMYNIGPVPWTCCISDFWKDGDQVFGGKVGKLLASYDDQGNSLSEFQTYVLNYYRCLWGSEYLQSITPLST