AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87585

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87585.t1 MTR_4g023090 71.340 321 72 4 1 304 69 386 1.39e-158 447
MS.gene87585.t1 MTR_4g023380 67.781 329 63 4 1 304 69 379 2.08e-149 424
MS.gene87585.t1 MTR_4g023080 71.382 304 74 4 1 303 62 353 4.66e-149 422
MS.gene87585.t1 MTR_4g023440 66.447 304 59 3 1 304 72 332 3.66e-135 386
MS.gene87585.t1 MTR_4g021785 65.686 306 91 4 1 304 62 355 2.38e-134 385
MS.gene87585.t1 MTR_4g023070 59.248 319 109 5 1 303 56 369 3.44e-123 357
MS.gene87585.t1 MTR_4g023130 64.894 282 87 3 23 303 42 312 1.56e-122 353
MS.gene87585.t1 MTR_4g023120 57.812 320 114 5 1 304 56 370 2.64e-120 350
MS.gene87585.t1 MTR_4g023190 60.984 305 102 7 3 302 65 357 6.67e-116 338
MS.gene87585.t1 MTR_4g023150 60.984 305 103 6 3 302 65 358 1.02e-115 338
MS.gene87585.t1 MTR_4g023200 59.609 307 108 6 1 302 63 358 9.86e-115 335
MS.gene87585.t1 MTR_3g103020 57.566 304 117 7 9 304 79 378 9.91e-105 310
MS.gene87585.t1 MTR_4g122850 56.721 305 120 10 9 304 63 364 2.01e-99 297
MS.gene87585.t1 MTR_4g117720 52.396 313 134 8 3 304 70 378 6.00e-94 283
MS.gene87585.t1 MTR_3g034180 52.997 317 129 9 3 304 178 489 5.51e-93 284
MS.gene87585.t1 MTR_4g109270 52.885 312 126 9 3 304 64 364 1.12e-92 279
MS.gene87585.t1 MTR_3g034160 53.333 315 129 8 3 303 64 374 2.85e-92 279
MS.gene87585.t1 MTR_4g094365 52.090 311 135 7 1 304 61 364 4.58e-92 278
MS.gene87585.t1 MTR_4g109280 53.074 309 129 7 3 304 60 359 8.38e-90 272
MS.gene87585.t1 MTR_8g433040 52.667 300 124 6 10 304 78 364 2.74e-89 271
MS.gene87585.t1 MTR_8g032380 52.667 300 124 6 10 304 78 364 2.74e-89 271
MS.gene87585.t1 MTR_6g060650 50.649 308 136 7 9 303 68 372 2.30e-88 268
MS.gene87585.t1 MTR_4g116330 53.558 267 111 6 44 304 121 380 2.00e-87 266
MS.gene87585.t1 MTR_3g034130 52.203 295 124 7 3 284 64 354 5.13e-86 263
MS.gene87585.t1 MTR_4g117770 51.935 310 120 8 11 304 71 367 9.80e-86 262
MS.gene87585.t1 MTR_4g117810 51.935 310 120 8 11 304 71 367 9.80e-86 262
MS.gene87585.t1 MTR_4g117780 51.316 304 131 8 11 304 71 367 4.37e-83 255
MS.gene87585.t1 MTR_4g117850 50.820 305 125 11 9 301 85 376 1.84e-82 254
MS.gene87585.t1 MTR_4g117870 49.495 297 107 8 12 304 10 267 2.97e-82 249
MS.gene87585.t1 MTR_5g070130 47.267 311 145 9 9 304 71 377 2.54e-81 251
MS.gene87585.t1 MTR_8g032390 49.158 297 120 8 9 299 99 370 7.73e-79 244
MS.gene87585.t1 MTR_4g118380 48.123 293 127 7 9 279 68 357 2.45e-76 237
MS.gene87585.t1 MTR_5g070080 46.626 326 135 12 9 304 70 386 4.02e-75 235
MS.gene87585.t1 MTR_5g097210 50.175 285 123 10 25 300 85 359 2.37e-71 225
MS.gene87585.t1 MTR_4g116320 57.209 215 83 5 94 301 43 255 2.36e-70 219
MS.gene87585.t1 MTR_5g070088 46.316 285 128 8 35 304 112 386 4.52e-70 222
MS.gene87585.t1 MTR_1g055135 43.408 311 153 9 9 304 73 375 4.77e-70 222
MS.gene87585.t1 MTR_1g055195 43.810 315 143 10 5 304 183 478 2.68e-69 223
MS.gene87585.t1 MTR_4g121580 46.284 296 120 10 8 294 74 339 1.11e-68 219
MS.gene87585.t1 MTR_8g433030 55.556 216 87 7 90 299 125 337 5.65e-66 210
MS.gene87585.t1 MTR_8g040935 54.545 209 85 5 105 304 1 208 8.08e-66 205
MS.gene87585.t1 MTR_4g116330 45.865 266 94 6 44 304 121 341 5.80e-65 207
MS.gene87585.t1 MTR_6g463430 57.005 207 77 6 105 304 1 202 1.79e-61 194
MS.gene87585.t1 MTR_4g118400 45.283 265 111 8 37 298 61 294 1.74e-60 194
MS.gene87585.t1 MTR_4g117750 44.551 312 139 11 9 303 64 358 4.42e-59 196
MS.gene87585.t1 MTR_4g117750 56.881 109 45 1 196 302 373 481 2.96e-28 114
MS.gene87585.t1 MTR_8g013010 51.707 205 93 4 3 205 109 309 8.10e-59 191
MS.gene87585.t1 MTR_7g079370 40.122 329 153 12 4 298 32 350 1.96e-57 189
MS.gene87585.t1 MTR_7g079400 41.837 294 146 9 26 304 54 337 1.05e-53 178
MS.gene87585.t1 MTR_4g118370 45.455 253 100 12 59 301 33 257 6.26e-52 171
MS.gene87585.t1 MTR_1g055165 37.417 302 130 8 9 304 70 318 5.35e-50 168
MS.gene87585.t1 MTR_4g114700 39.080 261 104 4 43 295 5 218 4.10e-49 164
MS.gene87585.t1 MTR_4g117530 40.984 244 104 5 64 304 47 253 2.36e-47 159
MS.gene87585.t1 MTR_4g117430 53.289 152 60 3 58 208 14 155 6.71e-44 147
MS.gene87585.t1 MTR_8g102520 39.464 261 135 8 57 304 72 322 1.41e-43 152
MS.gene87585.t1 MTR_2g048580 36.213 301 141 14 20 304 77 342 3.30e-42 149
MS.gene87585.t1 MTR_1g055250 39.224 232 118 8 78 291 27 253 1.23e-41 147
MS.gene87585.t1 MTR_1g055175 42.564 195 101 6 118 303 8 200 3.44e-39 137
MS.gene87585.t1 MTR_7g117720 51.408 142 63 4 9 146 52 191 3.27e-36 131
MS.gene87585.t1 MTR_7g117720 59.302 86 29 2 219 298 186 271 1.03e-20 90.1
MS.gene87585.t1 MTR_7g079420 36.000 275 125 11 46 304 905 1144 5.81e-36 137
MS.gene87585.t1 MTR_4g116340 50.000 150 68 4 28 172 100 247 2.79e-35 128
MS.gene87585.t1 MTR_5g023000 60.204 98 37 1 208 303 1 98 6.89e-33 117
MS.gene87585.t1 MTR_4g023430 73.171 82 17 1 228 304 141 222 3.34e-32 119
MS.gene87585.t1 MTR_4g118360 56.250 112 41 3 196 303 18 125 7.71e-31 112
MS.gene87585.t1 MTR_4g122860 38.318 214 94 8 104 304 132 320 4.48e-30 116
MS.gene87585.t1 MTR_4g133170 29.482 251 146 10 40 269 86 326 1.47e-24 102
MS.gene87585.t1 MTR_6g034720 42.553 141 76 4 70 206 53 192 2.14e-24 98.6
MS.gene87585.t1 MTR_0667s0010 28.159 277 163 12 25 271 75 345 2.08e-23 99.4
MS.gene87585.t1 MTR_8g098830 36.458 192 107 7 115 298 31 215 2.29e-23 96.3
MS.gene87585.t1 MTR_2g426070 29.021 286 173 11 44 303 105 386 6.26e-23 98.2
MS.gene87585.t1 MTR_2g025960 30.986 213 124 5 44 235 5 215 1.34e-22 95.9
MS.gene87585.t1 MTR_2g028130 27.642 246 152 9 44 266 106 348 2.02e-22 96.7
MS.gene87585.t1 MTR_2g426090 27.178 287 179 11 44 303 100 383 2.32e-22 96.7
MS.gene87585.t1 MTR_7g079450 41.135 141 75 4 167 304 2 137 3.91e-22 90.5
MS.gene87585.t1 MTR_2g425980 28.302 318 183 12 18 303 81 385 5.08e-22 95.5
MS.gene87585.t1 MTR_2g021150 27.609 297 196 13 10 296 81 368 5.39e-22 95.1
MS.gene87585.t1 MTR_2g028160 30.126 239 133 11 44 254 106 338 7.56e-21 92.4
MS.gene87585.t1 MTR_4g023100 78.182 55 12 0 250 304 65 119 9.38e-21 86.3
MS.gene87585.t1 MTR_1g068875 28.235 255 163 9 18 258 67 315 2.89e-20 90.1
MS.gene87585.t1 MTR_1g068835 26.506 249 165 8 40 273 87 332 2.94e-20 90.1
MS.gene87585.t1 MTR_4g070550 26.644 289 177 11 44 301 101 385 6.23e-20 89.7
MS.gene87585.t1 MTR_3g036290 30.451 266 148 13 42 296 117 356 7.49e-20 89.0
MS.gene87585.t1 MTR_2g426020 24.739 287 184 8 44 301 100 383 1.44e-19 88.6
MS.gene87585.t1 MTR_2g024110 28.421 285 170 15 44 301 93 370 1.98e-19 87.8
MS.gene87585.t1 MTR_2g025420 29.044 272 151 14 24 259 84 349 1.24e-18 85.9
MS.gene87585.t1 MTR_2g026010 28.472 288 175 13 40 301 86 368 1.26e-18 85.9
MS.gene87585.t1 MTR_1g097490 27.517 298 189 13 8 296 111 390 1.37e-18 85.5
MS.gene87585.t1 MTR_4g122840 43.382 136 38 5 173 304 164 264 2.89e-18 83.2
MS.gene87585.t1 MTR_4g117550 27.839 273 154 12 48 302 127 374 3.83e-18 84.3
MS.gene87585.t1 MTR_1g055200 50.588 85 34 3 43 125 5 83 4.42e-18 78.6
MS.gene87585.t1 MTR_8g099460 29.545 220 124 9 40 235 89 301 1.04e-17 83.2
MS.gene87585.t1 MTR_2g024100 28.279 244 144 10 19 235 71 310 1.29e-17 82.8
MS.gene87585.t1 MTR_0128s0020 28.421 285 163 10 42 295 118 392 2.98e-17 81.6
MS.gene87585.t1 MTR_7g407070 27.407 270 173 10 44 296 135 398 4.30e-17 81.3
MS.gene87585.t1 MTR_7g070880 28.125 288 144 12 42 273 114 394 4.43e-17 81.3
MS.gene87585.t1 MTR_5g007610 29.242 277 165 14 42 296 133 400 5.01e-17 81.3
MS.gene87585.t1 MTR_3g014570 26.688 311 191 13 7 296 84 378 1.16e-16 80.1
MS.gene87585.t1 MTR_2g028140 27.477 222 132 9 40 235 103 321 1.18e-16 80.1
MS.gene87585.t1 MTR_2g055970 25.373 268 183 9 38 296 301 560 1.49e-16 80.1
MS.gene87585.t1 MTR_2g031680 25.397 252 165 10 40 273 87 333 1.60e-16 79.7
MS.gene87585.t1 MTR_0129s0050 26.087 299 166 10 48 301 120 408 2.05e-16 79.3
MS.gene87585.t1 MTR_2g031720 26.644 289 183 13 39 304 91 373 2.18e-16 79.3
MS.gene87585.t1 MTR_3g007360 27.941 272 165 12 44 297 145 403 2.81e-16 79.0
MS.gene87585.t1 MTR_1g050542 25.926 270 174 13 42 296 146 404 3.50e-16 78.6
MS.gene87585.t1 MTR_4g055060 29.137 278 155 14 17 269 101 361 4.37e-16 78.2
MS.gene87585.t1 MTR_1g088120 27.372 274 139 11 44 269 109 370 5.28e-16 78.2
MS.gene87585.t1 MTR_2g426060 25.385 260 160 10 40 270 95 349 7.54e-16 77.8
MS.gene87585.t1 MTR_7g079640 25.658 304 188 12 5 292 84 365 1.16e-15 77.0
MS.gene87585.t1 MTR_7g070830 26.837 313 167 11 42 297 114 421 1.40e-15 77.0
MS.gene87585.t1 MTR_2g046900 26.797 306 192 16 9 296 93 384 1.48e-15 76.6
MS.gene87585.t1 MTR_2g046860 25.506 247 164 10 41 274 6 245 1.49e-15 76.3
MS.gene87585.t1 MTR_2g022000 24.468 282 176 9 45 293 130 407 1.94e-15 76.6
MS.gene87585.t1 MTR_1g094210 27.599 279 162 14 42 304 152 406 2.04e-15 76.6
MS.gene87585.t1 MTR_1g068860 26.277 274 174 11 25 277 72 338 2.05e-15 76.3
MS.gene87585.t1 MTR_3g007410 27.574 272 166 12 44 297 145 403 2.15e-15 76.3
MS.gene87585.t1 MTR_0129s0070 25.649 308 199 10 22 304 97 399 2.19e-15 76.3
MS.gene87585.t1 MTR_7g056503 26.829 287 174 11 41 296 116 397 3.02e-15 75.9
MS.gene87585.t1 MTR_2g021800 24.126 286 180 9 45 297 131 412 3.86e-15 75.9
MS.gene87585.t1 MTR_3g013740 27.106 273 164 13 44 296 149 406 4.55e-15 75.5
MS.gene87585.t1 MTR_2g021155 28.794 257 156 14 27 269 114 357 9.58e-15 75.1
MS.gene87585.t1 MTR_2g021155 31.156 199 110 10 88 269 738 926 4.61e-12 67.0
MS.gene87585.t1 MTR_2g104340 24.221 289 175 9 48 301 117 396 1.73e-14 73.6
MS.gene87585.t1 MTR_2g021050 26.148 283 159 12 18 273 68 327 1.78e-14 73.6
MS.gene87585.t1 MTR_1g094210 27.500 240 141 11 42 269 152 370 2.67e-14 73.2
MS.gene87585.t1 MTR_4g061340 28.947 228 120 11 31 236 109 316 3.41e-14 72.8
MS.gene87585.t1 MTR_3g011100 30.035 283 157 15 42 296 332 601 5.10e-14 72.8
MS.gene87585.t1 MTR_3g007430 28.837 215 134 7 32 235 122 328 5.45e-14 72.0
MS.gene87585.t1 MTR_3g007330 27.797 295 160 16 32 296 121 392 5.95e-14 72.0
MS.gene87585.t1 MTR_3g011060 27.759 299 174 16 15 295 111 385 1.31e-13 70.9
MS.gene87585.t1 MTR_4g130330 23.448 290 182 14 44 299 106 389 1.54e-13 70.9
MS.gene87585.t1 MTR_5g037680 26.923 234 150 9 35 259 117 338 1.70e-13 70.9
MS.gene87585.t1 MTR_1g055145 49.315 73 36 1 150 222 131 202 1.84e-13 69.7
MS.gene87585.t1 MTR_8g101580 24.638 276 192 9 30 296 126 394 2.20e-13 70.5
MS.gene87585.t1 MTR_8g013005 58.621 58 22 1 248 303 1 58 2.93e-13 63.9
MS.gene87585.t1 MTR_5g069390 25.952 289 185 11 35 301 136 417 2.98e-13 70.1
MS.gene87585.t1 MTR_1g054575 29.557 203 120 12 42 235 143 331 3.20e-13 70.5
MS.gene87585.t1 MTR_5g069120 39.423 104 57 2 9 107 52 154 3.29e-13 67.0
MS.gene87585.t1 MTR_8g041270 24.296 284 181 9 48 301 127 406 3.83e-13 69.7
MS.gene87585.t1 MTR_0044s0300 26.241 282 174 13 32 296 125 389 5.14e-13 69.3
MS.gene87585.t1 MTR_0707s0020 23.701 308 186 11 32 298 146 445 5.61e-13 69.3
MS.gene87585.t1 MTR_0129s0120 26.639 244 152 8 48 269 124 362 7.43e-13 68.9
MS.gene87585.t1 MTR_2g038680 25.079 315 174 14 31 299 111 409 9.46e-13 68.6
MS.gene87585.t1 MTR_7g070440 28.889 225 127 10 41 240 94 310 1.53e-12 67.8
MS.gene87585.t1 MTR_3g010390 25.000 256 170 9 44 282 136 386 1.54e-12 67.8
MS.gene87585.t1 MTR_0128s0070 26.923 286 165 11 42 295 122 395 1.59e-12 67.8
MS.gene87585.t1 MTR_6g053260 28.326 233 124 13 35 249 429 636 3.30e-12 67.4
MS.gene87585.t1 MTR_4g028660 24.823 282 179 12 44 297 119 395 3.65e-12 66.6
MS.gene87585.t1 MTR_4g027450 24.823 282 179 12 44 297 119 395 3.65e-12 66.6
MS.gene87585.t1 MTR_3g436150 28.253 269 165 15 45 296 135 392 4.01e-12 66.6
MS.gene87585.t1 MTR_7g062100 26.515 264 144 11 42 269 119 368 4.40e-12 66.6
MS.gene87585.t1 MTR_7g062060 25.000 304 194 12 21 297 104 400 5.14e-12 66.2
MS.gene87585.t1 MTR_3g011020 26.786 224 134 11 90 295 158 369 5.21e-12 66.2
MS.gene87585.t1 MTR_2g021190 26.441 295 183 15 20 296 94 372 5.50e-12 66.2
MS.gene87585.t1 MTR_6g065980 26.493 268 161 13 45 296 111 358 5.78e-12 65.9
MS.gene87585.t1 MTR_5g057710 28.165 316 182 17 4 296 101 394 6.64e-12 65.9
MS.gene87585.t1 MTR_4g107820 25.726 241 155 8 40 269 128 355 6.84e-12 65.9
MS.gene87585.t1 MTR_4g107780 25.726 241 155 8 40 269 128 355 6.84e-12 65.9
MS.gene87585.t1 MTR_0649s0020 26.174 298 160 15 44 296 105 387 7.12e-12 65.9
MS.gene87585.t1 MTR_4g107820 25.726 241 155 8 40 269 128 355 7.45e-12 65.9
MS.gene87585.t1 MTR_4g107780 25.726 241 155 8 40 269 128 355 8.63e-12 65.5
MS.gene87585.t1 MTR_7g077970 28.879 232 126 11 42 244 114 335 9.24e-12 65.5
MS.gene87585.t1 MTR_2g029990 25.746 268 173 12 42 296 148 402 1.07e-11 65.5
MS.gene87585.t1 MTR_7g017130 24.068 295 194 10 31 302 111 398 1.11e-11 65.5
MS.gene87585.t1 MTR_1004s0010 28.679 265 152 14 45 296 119 359 1.13e-11 65.1
MS.gene87585.t1 MTR_0760s0010 28.679 265 152 14 45 296 149 389 1.18e-11 65.1
MS.gene87585.t1 MTR_3g007390 27.854 219 132 10 88 296 1 203 1.20e-11 63.2
MS.gene87585.t1 MTR_5g009060 26.882 279 154 15 44 296 144 398 1.24e-11 65.1
MS.gene87585.t1 MTR_7g017040 22.772 303 176 14 41 302 114 399 1.62e-11 64.7
MS.gene87585.t1 MTR_6g027910 26.644 289 171 14 35 296 100 374 1.77e-11 64.7
MS.gene87585.t1 MTR_2g046830 22.383 277 190 10 4 269 85 347 2.21e-11 64.3
MS.gene87585.t1 MTR_4g013180 26.103 272 146 12 44 271 104 364 2.68e-11 64.3
MS.gene87585.t1 MTR_3g009240 26.222 225 147 8 58 272 146 361 2.85e-11 63.9
MS.gene87585.t1 MTR_7g017170 25.664 226 143 8 31 235 111 332 2.99e-11 63.9
MS.gene87585.t1 MTR_5g096710 26.792 265 145 12 41 269 118 369 3.00e-11 63.9
MS.gene87585.t1 MTR_5g059430 24.809 262 170 10 35 275 142 397 3.46e-11 63.9
MS.gene87585.t1 MTR_8g063440 26.582 237 143 9 40 256 111 336 3.92e-11 63.5
MS.gene87585.t1 MTR_3g007270 24.719 267 175 10 10 263 121 374 4.03e-11 63.5
MS.gene87585.t1 MTR_0128s0010 28.231 294 163 15 40 296 115 397 4.20e-11 63.5
MS.gene87585.t1 MTR_3g030350 28.214 280 154 14 44 296 131 390 4.81e-11 63.2
MS.gene87585.t1 MTR_7g069550 27.734 256 138 13 44 266 123 364 4.92e-11 63.2
MS.gene87585.t1 MTR_0054s0090 22.789 294 179 11 44 299 121 404 5.14e-11 63.2
MS.gene87585.t1 MTR_5g064700 21.661 277 176 9 60 301 98 368 5.41e-11 63.2
MS.gene87585.t1 MTR_6g086825 24.415 299 190 11 36 302 167 461 5.64e-11 63.2
MS.gene87585.t1 MTR_3g083640 25.540 278 173 14 33 296 145 402 5.98e-11 63.2
MS.gene87585.t1 MTR_3g009260 27.638 199 127 6 44 236 132 319 7.32e-11 62.8
MS.gene87585.t1 MTR_6g017295 26.183 317 179 15 3 275 1 306 8.85e-11 62.8
MS.gene87585.t1 MTR_7g009265 26.070 257 146 12 41 258 30 281 9.48e-11 62.0
MS.gene87585.t1 MTR_1g112010 23.950 238 153 7 42 269 76 295 9.89e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87585.t1 AT3G06240 31.224 237 145 9 40 269 163 388 3.13e-20 90.5
MS.gene87585.t1 AT4G12560 28.767 292 173 12 39 300 88 374 2.89e-18 84.7
MS.gene87585.t1 AT4G12560 28.767 292 173 12 39 300 88 374 2.89e-18 84.7
MS.gene87585.t1 AT4G12560 28.767 292 173 12 39 300 88 374 2.89e-18 84.7
MS.gene87585.t1 AT4G22390 27.386 241 148 9 40 258 89 324 9.55e-15 74.3
MS.gene87585.t1 AT4G22390 27.386 241 148 9 40 258 89 324 1.12e-14 74.3
MS.gene87585.t1 AT3G23880 28.102 274 162 18 41 300 111 363 2.45e-14 73.2

Find 57 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGTTGCCTTTGATTTAA+TGG 0.135224 4.1:-74907487 MS.gene87585:CDS
GCGACATTGTGCTACTAATT+TGG 0.142162 4.1:+74907758 None:intergenic
ATTACATTCCAACGTAAATT+AGG 0.238514 4.1:+74907507 None:intergenic
TTGGAGAATTTCTCAGTCTA+TGG 0.267319 4.1:-74907395 MS.gene87585:CDS
AGAAAAGGGTGTCTATTTAA+TGG 0.280867 4.1:-74907555 MS.gene87585:CDS
ATAAGGATACAGTTGAAATA+TGG 0.287555 4.1:-74907359 MS.gene87585:CDS
ATGATAAAGGACAGTTATTA+AGG 0.300794 4.1:-74907185 MS.gene87585:CDS
CGCGTTCATGTACATGGTTT+TGG 0.311272 4.1:-74907738 MS.gene87585:CDS
GAAAAGGGTGTCTATTTAAT+GGG 0.320229 4.1:-74907554 MS.gene87585:CDS
TCTCATGAAATTCGATCTAT+AGG 0.322760 4.1:-74907972 MS.gene87585:CDS
TATTTAATGGGGCTATTCAT+TGG 0.344728 4.1:-74907542 MS.gene87585:CDS
TTGTTTGAGATGCCTCTTCC+AGG 0.350585 4.1:-74907456 MS.gene87585:CDS
ATCTGATGTTGTCATGTATA+CGG 0.404158 4.1:-74907139 MS.gene87585:CDS
AAGAAATCTTAGTTATAGAT+TGG 0.412950 4.1:-74907217 MS.gene87585:CDS
TGATAAGGAACAACAGCATA+AGG 0.415699 4.1:+74907579 None:intergenic
GTTGTTCCTTATCACAGAAA+AGG 0.416894 4.1:-74907570 MS.gene87585:CDS
CACGAACCTCTAATTTCAAC+CGG 0.420465 4.1:+74907876 None:intergenic
CAATGTCGCGTTCATGTACA+TGG 0.438350 4.1:-74907744 MS.gene87585:CDS
GGATTCCATATGTAGATGTC+TGG 0.447584 4.1:+74907825 None:intergenic
ATTAGCTACGGATGGATCAT+AGG 0.449088 4.1:+74907674 None:intergenic
GCTACTAATTTGGAATCAAA+AGG 0.455580 4.1:+74907768 None:intergenic
TTGGTCATGTATAATGATAA+AGG 0.457724 4.1:-74907198 MS.gene87585:CDS
TAAGGATACAGTTGAAATAT+GGG 0.473963 4.1:-74907358 MS.gene87585:CDS
TTTCCTTTCCATTAAATCAA+AGG 0.474723 4.1:+74907479 None:intergenic
TTGTTCCTTATCACAGAAAA+GGG 0.480238 4.1:-74907569 MS.gene87585:CDS
AATACAAAGTGTATTCGTCT+TGG 0.488178 4.1:-74907326 MS.gene87585:CDS
TGGAGAATTTCTCAGTCTAT+GGG 0.489976 4.1:-74907394 MS.gene87585:CDS
AAATTAAGGTTCAGTGAAGC+AGG 0.496308 4.1:+74907921 None:intergenic
CCGGTCAAGAGATGATTACT+TGG 0.500070 4.1:-74907709 MS.gene87585:CDS
TAATACATGGAAAGTAATTG+AGG 0.504046 4.1:-74907613 MS.gene87585:CDS
ATGGGCTGTGGACTATGATA+AGG 0.510847 4.1:-74907376 MS.gene87585:CDS
ATAGGGTGAAAATAGCGATC+AGG 0.512584 4.1:+74907270 None:intergenic
AAATTAGAGGTTCGTGTAGA+GGG 0.519047 4.1:-74907869 MS.gene87585:CDS
GAAATTAGAGGTTCGTGTAG+AGG 0.526536 4.1:-74907870 MS.gene87585:CDS
TTGCCTTTGATTTAATGGAA+AGG 0.533360 4.1:-74907482 MS.gene87585:CDS
GTCAAGAGATGATTACTTGG+TGG 0.541832 4.1:-74907706 MS.gene87585:CDS
CTTTGCTTCCTAATTTACGT+TGG 0.542961 4.1:-74907515 MS.gene87585:CDS
ATCAGATGGACTATTAAAGA+AGG 0.544756 4.1:+74907155 None:intergenic
GATAAGGAACAACAGCATAA+GGG 0.545954 4.1:+74907580 None:intergenic
TCTCAGTTAGAGATAATACA+TGG 0.549279 4.1:-74907626 MS.gene87585:CDS
TACATATGGAATCCATCCAC+TGG 0.550790 4.1:-74907816 MS.gene87585:CDS
GACGTCCAGACATCTACATA+TGG 0.552487 4.1:-74907830 MS.gene87585:CDS
ATTTCTCAGTCTATGGGCTG+TGG 0.553738 4.1:-74907388 MS.gene87585:CDS
AATACATGGAAAGTAATTGA+GGG 0.565339 4.1:-74907612 MS.gene87585:CDS
AGGTTAAAATCACCTGGAAG+AGG 0.570122 4.1:+74907444 None:intergenic
GTGAAAATAGCGATCAGGAA+TGG 0.580924 4.1:+74907275 None:intergenic
CCAAGTAATCATCTCTTGAC+CGG 0.585969 4.1:+74907709 None:intergenic
AGTAAAAGGTTAAAATCACC+TGG 0.587427 4.1:+74907438 None:intergenic
TAAACTTGCTCATTGTCACC+AGG 0.594919 4.1:+74907099 None:intergenic
GAAACAGCATTAGCTACGGA+TGG 0.602626 4.1:+74907666 None:intergenic
TTGGAATCAAAAGGAGACAC+GGG 0.612354 4.1:+74907777 None:intergenic
TGTTGAAACAGCATTAGCTA+CGG 0.620181 4.1:+74907662 None:intergenic
GTATACATGACAACATCAGA+TGG 0.627287 4.1:+74907141 None:intergenic
GAGTCTCTACTTTCACTACC+TGG 0.642620 4.1:-74907117 MS.gene87585:CDS
AAAAGGGTGTCTATTTAATG+GGG 0.654843 4.1:-74907553 MS.gene87585:CDS
TTTGGAATCAAAAGGAGACA+CGG 0.662326 4.1:+74907776 None:intergenic
ATATGTAGATGTCTGGACGT+CGG 0.736771 4.1:+74907832 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACCAGAATTAAGTAAA+AGG + chr4.1:74907667-74907686 None:intergenic 20.0%
!! AAGAAATCTTAGTTATAGAT+TGG - chr4.1:74907871-74907890 MS.gene87585:CDS 20.0%
!!! TAATTGTTTTTTAATTCCAG+TGG + chr4.1:74907291-74907310 None:intergenic 20.0%
!!! TTTAACCTTTTACTTAATTC+TGG - chr4.1:74907659-74907678 MS.gene87585:CDS 20.0%
!!! TTTACTTAATTCTGGTTTTT+GGG - chr4.1:74907667-74907686 MS.gene87585:CDS 20.0%
!!! TTTTACTTAATTCTGGTTTT+TGG - chr4.1:74907666-74907685 MS.gene87585:CDS 20.0%
! AATACATGGAAAGTAATTGA+GGG - chr4.1:74907476-74907495 MS.gene87585:CDS 25.0%
! ATAAGGATACAGTTGAAATA+TGG - chr4.1:74907729-74907748 MS.gene87585:CDS 25.0%
! ATGATAAAGGACAGTTATTA+AGG - chr4.1:74907903-74907922 MS.gene87585:CDS 25.0%
! ATTACATTCCAACGTAAATT+AGG + chr4.1:74907584-74907603 None:intergenic 25.0%
! ATTCTGTAAGCGAAAAATTA+AGG + chr4.1:74907184-74907203 None:intergenic 25.0%
! TAAGGATACAGTTGAAATAT+GGG - chr4.1:74907730-74907749 MS.gene87585:CDS 25.0%
! TAATACATGGAAAGTAATTG+AGG - chr4.1:74907475-74907494 MS.gene87585:CDS 25.0%
! TTGGTCATGTATAATGATAA+AGG - chr4.1:74907890-74907909 MS.gene87585:CDS 25.0%
! TTTCCTTTCCATTAAATCAA+AGG + chr4.1:74907612-74907631 None:intergenic 25.0%
!! AATTGTTGCCTTTGATTTAA+TGG - chr4.1:74907601-74907620 MS.gene87585:CDS 25.0%
!!! AATAATATCACCGTTTTTTG+TGG + chr4.1:74907849-74907868 None:intergenic 25.0%
AATACAAAGTGTATTCGTCT+TGG - chr4.1:74907762-74907781 MS.gene87585:CDS 30.0%
AGTAAAAGGTTAAAATCACC+TGG + chr4.1:74907653-74907672 None:intergenic 30.0%
ATCTGATGTTGTCATGTATA+CGG - chr4.1:74907949-74907968 MS.gene87585:CDS 30.0%
TATTTAATGGGGCTATTCAT+TGG - chr4.1:74907546-74907565 MS.gene87585:CDS 30.0%
TCTCAGTTAGAGATAATACA+TGG - chr4.1:74907462-74907481 MS.gene87585:CDS 30.0%
TCTCATGAAATTCGATCTAT+AGG - chr4.1:74907116-74907135 MS.gene87585:CDS 30.0%
TTGTTCCTTATCACAGAAAA+GGG - chr4.1:74907519-74907538 MS.gene87585:CDS 30.0%
! GCTACTAATTTGGAATCAAA+AGG + chr4.1:74907323-74907342 None:intergenic 30.0%
! TATTTTCCGGTTGAAATTAG+AGG - chr4.1:74907206-74907225 MS.gene87585:CDS 30.0%
! TTGCCTTTGATTTAATGGAA+AGG - chr4.1:74907606-74907625 MS.gene87585:CDS 30.0%
!! AAAAGGGTGTCTATTTAATG+GGG - chr4.1:74907535-74907554 MS.gene87585:CDS 30.0%
!! AGAAAAGGGTGTCTATTTAA+TGG - chr4.1:74907533-74907552 MS.gene87585:CDS 30.0%
!! ATCAGATGGACTATTAAAGA+AGG + chr4.1:74907936-74907955 None:intergenic 30.0%
!! GAAAAGGGTGTCTATTTAAT+GGG - chr4.1:74907534-74907553 MS.gene87585:CDS 30.0%
!!! AATTCTGGTTTTTGGGTATT+TGG - chr4.1:74907674-74907693 MS.gene87585:CDS 30.0%
!!! GCTTACAGAATCTTATTTTC+CGG - chr4.1:74907193-74907212 MS.gene87585:CDS 30.0%
!!! TGTTTTTTAATTCCAGTGGA+TGG + chr4.1:74907287-74907306 None:intergenic 30.0%
AAATTAAGGTTCAGTGAAGC+AGG + chr4.1:74907170-74907189 None:intergenic 35.0%
AAATTAGAGGTTCGTGTAGA+GGG - chr4.1:74907219-74907238 MS.gene87585:CDS 35.0%
CCTATGTACTCCACAAAAAA+CGG - chr4.1:74907836-74907855 MS.gene87585:CDS 35.0%
CTTTGCTTCCTAATTTACGT+TGG - chr4.1:74907573-74907592 MS.gene87585:CDS 35.0%
GATAAGGAACAACAGCATAA+GGG + chr4.1:74907511-74907530 None:intergenic 35.0%
GTATACATGACAACATCAGA+TGG + chr4.1:74907950-74907969 None:intergenic 35.0%
GTTGTTCCTTATCACAGAAA+AGG - chr4.1:74907518-74907537 MS.gene87585:CDS 35.0%
TGATAAGGAACAACAGCATA+AGG + chr4.1:74907512-74907531 None:intergenic 35.0%
TGGAGAATTTCTCAGTCTAT+GGG - chr4.1:74907694-74907713 MS.gene87585:CDS 35.0%
TGTTGAAACAGCATTAGCTA+CGG + chr4.1:74907429-74907448 None:intergenic 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr4.1:74907693-74907712 MS.gene87585:CDS 35.0%
! TTTGGAATCAAAAGGAGACA+CGG + chr4.1:74907315-74907334 None:intergenic 35.0%
!!! CGTTTTTTGTGGAGTACATA+GGG + chr4.1:74907838-74907857 None:intergenic 35.0%
!!! TACATGGTTTTGGTTATGAC+CGG - chr4.1:74907360-74907379 MS.gene87585:CDS 35.0%
AGGTTAAAATCACCTGGAAG+AGG + chr4.1:74907647-74907666 None:intergenic 40.0%
ATAGGGTGAAAATAGCGATC+AGG + chr4.1:74907821-74907840 None:intergenic 40.0%
ATATGTAGATGTCTGGACGT+CGG + chr4.1:74907259-74907278 None:intergenic 40.0%
ATTAGCTACGGATGGATCAT+AGG + chr4.1:74907417-74907436 None:intergenic 40.0%
CACGAACCTCTAATTTCAAC+CGG + chr4.1:74907215-74907234 None:intergenic 40.0%
CCAAGTAATCATCTCTTGAC+CGG + chr4.1:74907382-74907401 None:intergenic 40.0%
GAAATTAGAGGTTCGTGTAG+AGG - chr4.1:74907218-74907237 MS.gene87585:CDS 40.0%
GGATTCCATATGTAGATGTC+TGG + chr4.1:74907266-74907285 None:intergenic 40.0%
GTCAAGAGATGATTACTTGG+TGG - chr4.1:74907382-74907401 MS.gene87585:CDS 40.0%
GTGAAAATAGCGATCAGGAA+TGG + chr4.1:74907816-74907835 None:intergenic 40.0%
TACATATGGAATCCATCCAC+TGG - chr4.1:74907272-74907291 MS.gene87585:CDS 40.0%
TTGGAATCAAAAGGAGACAC+GGG + chr4.1:74907314-74907333 None:intergenic 40.0%
! AGACACCCTTTTCTGTGATA+AGG + chr4.1:74907527-74907546 None:intergenic 40.0%
! GCGACATTGTGCTACTAATT+TGG + chr4.1:74907333-74907352 None:intergenic 40.0%
! TAAACTTGCTCATTGTCACC+AGG + chr4.1:74907992-74908011 None:intergenic 40.0%
!!! CCGTTTTTTGTGGAGTACAT+AGG + chr4.1:74907839-74907858 None:intergenic 40.0%
CAATGTCGCGTTCATGTACA+TGG - chr4.1:74907344-74907363 MS.gene87585:CDS 45.0%
CCGGTCAAGAGATGATTACT+TGG - chr4.1:74907379-74907398 MS.gene87585:CDS 45.0%
GAAACAGCATTAGCTACGGA+TGG + chr4.1:74907425-74907444 None:intergenic 45.0%
GACGTCCAGACATCTACATA+TGG - chr4.1:74907258-74907277 MS.gene87585:CDS 45.0%
GAGTCTCTACTTTCACTACC+TGG - chr4.1:74907971-74907990 MS.gene87585:CDS 45.0%
! ATGGGCTGTGGACTATGATA+AGG - chr4.1:74907712-74907731 MS.gene87585:CDS 45.0%
! ATTTCTCAGTCTATGGGCTG+TGG - chr4.1:74907700-74907719 MS.gene87585:CDS 45.0%
! CGCGTTCATGTACATGGTTT+TGG - chr4.1:74907350-74907369 MS.gene87585:CDS 45.0%
! TTGTTTGAGATGCCTCTTCC+AGG - chr4.1:74907632-74907651 MS.gene87585:CDS 45.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 74907098 74908012 74907098 ID=MS.gene87585
chr4.1 mRNA 74907098 74908012 74907098 ID=MS.gene87585.t1;Parent=MS.gene87585
chr4.1 exon 74907098 74908012 74907098 ID=MS.gene87585.t1.exon1;Parent=MS.gene87585.t1
chr4.1 CDS 74907098 74908012 74907098 ID=cds.MS.gene87585.t1;Parent=MS.gene87585.t1
Gene Sequence

>MS.gene87585

ATGTGCATATCATCTCTATCTCATGAAATTCGATCTATAGGTTTTGAAGCATTTCTTAACGATGATGATCCTGCTTCACTGAACCTTAATTTTTCGCTTACAGAATCTTATTTTCCGGTTGAAATTAGAGGTTCGTGTAGAGGGTTTATACTTTTGTACCGACGTCCAGACATCTACATATGGAATCCATCCACTGGAATTAAAAAACAATTACCCGTGTCTCCTTTTGATTCCAAATTAGTAGCACAATGTCGCGTTCATGTACATGGTTTTGGTTATGACCGGTCAAGAGATGATTACTTGGTGGTTTCATTGTCCTATGATCCATCCGTAGCTAATGCTGTTTCAACACATTTGAAGTTTTTCTCAGTTAGAGATAATACATGGAAAGTAATTGAGGGTACTCACTTCCCTTATGCTGTTGTTCCTTATCACAGAAAAGGGTGTCTATTTAATGGGGCTATTCATTGGTTGACTTTGCTTCCTAATTTACGTTGGAATGTAATTGTTGCCTTTGATTTAATGGAAAGGAAATTGTTTGAGATGCCTCTTCCAGGTGATTTTAACCTTTTACTTAATTCTGGTTTTTGGGTATTTGGAGAATTTCTCAGTCTATGGGCTGTGGACTATGATAAGGATACAGTTGAAATATGGGTGATGAAAGAATACAAAGTGTATTCGTCTTGGAATAAAATTCTTGTTCTTTCTGTTCATGCCATTCCTGATCGCTATTTTCACCCTATGTACTCCACAAAAAACGGTGATATTATTGTAAGAAATCTTAGTTATAGATTGGTCATGTATAATGATAAAGGACAGTTATTAAGGCATCACTCCTTCTTTAATAGTCCATCTGATGTTGTCATGTATACGGAGTCTCTACTTTCACTACCTGGTGACAATGAGCAAGTTTAA
Protein sequence

>MS.gene87585.t1

MCISSLSHEIRSIGFEAFLNDDDPASLNLNFSLTESYFPVEIRGSCRGFILLYRRPDIYIWNPSTGIKKQLPVSPFDSKLVAQCRVHVHGFGYDRSRDDYLVVSLSYDPSVANAVSTHLKFFSVRDNTWKVIEGTHFPYAVVPYHRKGCLFNGAIHWLTLLPNLRWNVIVAFDLMERKLFEMPLPGDFNLLLNSGFWVFGEFLSLWAVDYDKDTVEIWVMKEYKVYSSWNKILVLSVHAIPDRYFHPMYSTKNGDIIVRNLSYRLVMYNDKGQLLRHHSFFNSPSDVVMYTESLLSLPGDNEQV