AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43789

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43789.t1 MTR_5g070080 88.686 274 27 1 6 275 113 386 3.29e-177 493
MS.gene43789.t1 MTR_5g070088 65.468 278 87 5 6 275 110 386 1.77e-121 352
MS.gene43789.t1 MTR_5g070130 62.847 288 92 6 1 275 92 377 1.41e-115 337
MS.gene43789.t1 MTR_3g103020 56.383 282 113 6 1 275 100 378 1.32e-95 286
MS.gene43789.t1 MTR_4g117720 55.000 280 108 8 10 275 103 378 1.02e-89 271
MS.gene43789.t1 MTR_6g060650 51.852 270 117 7 13 274 108 372 7.74e-79 243
MS.gene43789.t1 MTR_3g034180 49.658 292 121 9 1 274 205 488 7.12e-77 241
MS.gene43789.t1 MTR_5g097210 50.719 278 118 10 2 269 90 358 4.23e-76 236
MS.gene43789.t1 MTR_3g034160 50.685 292 118 10 1 274 91 374 9.70e-75 233
MS.gene43789.t1 MTR_1g055195 50.923 271 103 8 12 268 218 472 9.88e-74 233
MS.gene43789.t1 MTR_4g109270 51.079 278 110 9 12 275 99 364 1.09e-73 229
MS.gene43789.t1 MTR_4g023080 48.364 275 126 7 1 271 89 351 1.10e-73 229
MS.gene43789.t1 MTR_1g055135 49.135 289 124 10 2 275 95 375 2.35e-73 229
MS.gene43789.t1 MTR_4g109280 49.645 282 129 7 1 275 84 359 2.88e-72 226
MS.gene43789.t1 MTR_4g117850 50.562 267 117 6 12 267 111 373 2.26e-71 224
MS.gene43789.t1 MTR_4g023090 45.791 297 130 10 1 274 97 385 6.78e-70 221
MS.gene43789.t1 MTR_3g034130 48.375 277 119 8 2 260 92 362 7.25e-70 220
MS.gene43789.t1 MTR_4g094365 46.182 275 130 8 5 268 91 358 9.97e-70 219
MS.gene43789.t1 MTR_4g023130 48.837 258 117 6 19 272 65 311 1.79e-69 217
MS.gene43789.t1 MTR_4g117750 48.014 277 119 8 10 269 86 354 3.33e-68 219
MS.gene43789.t1 MTR_4g117750 55.357 112 49 1 159 269 367 478 2.39e-26 107
MS.gene43789.t1 MTR_4g118380 48.438 256 111 6 9 247 106 357 1.48e-66 211
MS.gene43789.t1 MTR_4g122850 45.035 282 137 10 1 269 83 359 9.82e-66 210
MS.gene43789.t1 MTR_4g117770 47.368 285 133 9 1 275 90 367 1.49e-65 209
MS.gene43789.t1 MTR_4g117810 47.368 285 133 9 1 275 90 367 1.49e-65 209
MS.gene43789.t1 MTR_4g117780 46.316 285 136 9 1 275 90 367 2.23e-64 206
MS.gene43789.t1 MTR_4g023190 46.071 280 129 10 1 271 90 356 3.73e-64 205
MS.gene43789.t1 MTR_4g023150 45.357 280 132 10 1 271 90 357 3.13e-63 202
MS.gene43789.t1 MTR_4g021785 46.125 271 129 9 10 275 97 355 4.08e-63 202
MS.gene43789.t1 MTR_8g032390 45.985 274 131 8 10 270 102 371 6.29e-63 202
MS.gene43789.t1 MTR_4g023070 45.424 295 123 12 4 272 86 368 6.32e-63 202
MS.gene43789.t1 MTR_4g023380 45.277 307 112 13 1 275 97 379 7.98e-63 202
MS.gene43789.t1 MTR_4g023120 45.085 295 124 11 4 272 86 368 2.12e-62 201
MS.gene43789.t1 MTR_4g116330 48.339 271 117 10 17 275 121 380 2.32e-62 201
MS.gene43789.t1 MTR_4g023200 45.357 280 132 10 1 271 90 357 8.08e-62 199
MS.gene43789.t1 MTR_4g023440 47.601 271 95 11 8 275 106 332 1.58e-61 197
MS.gene43789.t1 MTR_4g118400 46.008 263 115 7 7 268 58 294 2.05e-61 196
MS.gene43789.t1 MTR_8g433040 44.444 279 130 9 7 275 101 364 5.87e-60 194
MS.gene43789.t1 MTR_8g032380 44.444 279 130 9 7 275 101 364 5.87e-60 194
MS.gene43789.t1 MTR_4g118370 46.371 248 98 8 32 269 33 255 1.76e-59 190
MS.gene43789.t1 MTR_7g079400 44.014 284 129 10 6 270 61 333 1.91e-56 184
MS.gene43789.t1 MTR_4g116320 52.336 214 90 6 65 269 43 253 2.86e-56 181
MS.gene43789.t1 MTR_4g117870 43.774 265 106 8 10 269 36 262 5.66e-56 181
MS.gene43789.t1 MTR_8g433030 50.000 218 97 6 61 270 125 338 1.86e-54 179
MS.gene43789.t1 MTR_7g079370 43.011 279 132 11 14 270 79 352 3.67e-53 177
MS.gene43789.t1 MTR_4g121580 44.195 267 105 10 10 264 105 339 6.58e-52 174
MS.gene43789.t1 MTR_8g102520 42.248 258 128 10 31 275 73 322 2.67e-51 171
MS.gene43789.t1 MTR_1g055165 40.569 281 105 10 2 275 93 318 9.27e-50 167
MS.gene43789.t1 MTR_4g117530 40.727 275 113 8 6 275 24 253 5.52e-49 162
MS.gene43789.t1 MTR_4g116330 43.820 267 96 11 17 275 121 341 7.78e-48 162
MS.gene43789.t1 MTR_8g013010 49.153 177 84 4 1 174 136 309 1.56e-47 161
MS.gene43789.t1 MTR_1g055175 48.485 198 88 8 88 275 8 201 1.63e-47 157
MS.gene43789.t1 MTR_6g463430 47.115 208 96 5 76 275 1 202 2.25e-47 157
MS.gene43789.t1 MTR_1g055250 45.714 210 95 8 54 248 37 242 2.93e-47 160
MS.gene43789.t1 MTR_8g040935 46.190 210 101 6 76 275 1 208 1.93e-41 142
MS.gene43789.t1 MTR_4g117430 49.689 161 66 5 20 175 3 153 2.58e-40 137
MS.gene43789.t1 MTR_7g079420 38.129 278 113 12 19 275 905 1144 7.70e-39 144
MS.gene43789.t1 MTR_2g048580 35.764 288 139 17 1 275 88 342 8.80e-37 133
MS.gene43789.t1 MTR_4g114700 40.927 259 98 10 16 265 6 218 1.52e-36 130
MS.gene43789.t1 MTR_5g023000 62.000 100 36 2 177 275 1 99 1.78e-31 112
MS.gene43789.t1 MTR_4g116340 45.985 137 68 3 13 144 112 247 1.09e-30 115
MS.gene43789.t1 MTR_4g118360 54.867 113 44 4 159 268 12 120 2.18e-28 105
MS.gene43789.t1 MTR_8g098830 40.123 162 84 4 91 243 37 194 2.20e-27 106
MS.gene43789.t1 MTR_7g079450 45.390 141 68 6 139 275 2 137 9.62e-24 94.0
MS.gene43789.t1 MTR_7g117720 53.271 107 42 3 171 269 166 272 6.39e-23 95.5
MS.gene43789.t1 MTR_7g117720 51.546 97 42 3 13 105 85 180 1.39e-22 94.4
MS.gene43789.t1 MTR_4g122860 37.222 180 88 3 93 268 156 314 3.38e-20 89.0
MS.gene43789.t1 MTR_1g055200 51.190 84 36 2 16 97 5 85 1.28e-19 82.0
MS.gene43789.t1 MTR_2g024110 29.469 207 124 8 11 200 88 289 2.04e-19 87.0
MS.gene43789.t1 MTR_4g122840 41.538 130 37 2 146 270 165 260 2.10e-19 85.5
MS.gene43789.t1 MTR_2g026010 30.088 226 128 9 17 216 90 311 1.25e-18 85.1
MS.gene43789.t1 MTR_4g133170 27.642 246 152 10 13 237 86 326 3.15e-18 84.0
MS.gene43789.t1 MTR_2g028160 31.556 225 132 9 21 226 120 341 7.71e-18 83.2
MS.gene43789.t1 MTR_1g068835 27.830 212 128 10 13 205 87 292 4.03e-17 80.5
MS.gene43789.t1 MTR_6g034720 40.833 120 63 4 43 156 53 170 1.30e-16 77.0
MS.gene43789.t1 MTR_2g031720 27.719 285 180 10 11 272 90 371 1.30e-16 79.3
MS.gene43789.t1 MTR_4g023430 54.217 83 33 3 197 275 141 222 1.32e-16 77.0
MS.gene43789.t1 MTR_7g070830 28.616 318 152 16 15 267 114 421 1.39e-16 79.3
MS.gene43789.t1 MTR_2g025960 29.954 217 117 9 17 204 5 215 1.66e-16 78.2
MS.gene43789.t1 MTR_4g070550 29.661 236 124 10 13 212 97 326 1.76e-16 79.0
MS.gene43789.t1 MTR_7g070880 27.358 318 156 16 15 267 114 421 8.89e-16 77.0
MS.gene43789.t1 MTR_2g024100 28.829 222 119 9 12 200 91 306 1.20e-15 76.6
MS.gene43789.t1 MTR_0667s0010 26.749 243 127 10 8 212 85 314 1.21e-15 76.6
MS.gene43789.t1 MTR_2g426090 27.966 236 133 11 12 216 95 324 1.28e-15 76.6
MS.gene43789.t1 MTR_4g013175 28.028 289 136 13 15 251 121 389 2.07e-15 75.9
MS.gene43789.t1 MTR_3g036290 32.836 201 104 10 14 205 116 294 2.21e-15 75.5
MS.gene43789.t1 MTR_2g426070 28.696 230 127 9 17 216 105 327 3.90e-15 75.1
MS.gene43789.t1 MTR_2g078870 27.979 193 109 8 19 200 59 232 4.82e-15 73.9
MS.gene43789.t1 MTR_1g068875 26.383 235 147 10 12 226 86 314 6.83e-15 74.3
MS.gene43789.t1 MTR_2g031640 26.877 253 155 12 1 229 76 322 3.46e-14 72.0
MS.gene43789.t1 MTR_2g426060 27.711 249 136 11 4 216 86 326 3.71e-14 72.0
MS.gene43789.t1 MTR_1g068860 27.830 212 130 9 13 205 87 294 5.85e-14 71.6
MS.gene43789.t1 MTR_2g028130 27.511 229 116 11 13 205 102 316 9.14e-14 70.9
MS.gene43789.t1 MTR_6g080680 24.335 263 156 10 33 266 142 390 1.59e-13 70.1
MS.gene43789.t1 MTR_5g069120 43.158 95 43 3 14 97 83 177 2.18e-13 67.4
MS.gene43789.t1 MTR_3g007410 28.750 240 139 12 9 233 142 364 2.40e-13 69.7
MS.gene43789.t1 MTR_4g028660 23.050 282 156 11 28 267 133 395 2.96e-13 69.3
MS.gene43789.t1 MTR_4g027450 23.050 282 156 11 28 267 133 395 2.96e-13 69.3
MS.gene43789.t1 MTR_2g031680 25.118 211 134 7 13 204 87 292 8.57e-13 68.2
MS.gene43789.t1 MTR_6g017305 26.702 191 125 5 30 206 14 203 1.25e-12 67.0
MS.gene43789.t1 MTR_5g043605 27.857 280 171 13 13 273 72 339 2.88e-12 66.6
MS.gene43789.t1 MTR_3g030250 29.000 200 112 9 13 204 133 310 2.89e-12 66.6
MS.gene43789.t1 MTR_2g021155 28.992 238 142 11 17 237 130 357 3.06e-12 67.0
MS.gene43789.t1 MTR_2g021050 27.404 208 122 9 13 204 91 285 5.11e-12 65.9
MS.gene43789.t1 MTR_2g055970 27.619 210 133 7 15 216 305 503 5.15e-12 66.2
MS.gene43789.t1 MTR_2g021150 24.335 263 181 8 15 266 113 368 5.27e-12 65.9
MS.gene43789.t1 MTR_8g013005 61.404 57 20 2 220 275 4 59 6.01e-12 60.1
MS.gene43789.t1 MTR_0064s0160 25.926 297 187 12 3 274 139 427 1.15e-11 64.7
MS.gene43789.t1 MTR_4g109290 60.417 48 18 1 29 76 89 135 1.22e-11 62.8
MS.gene43789.t1 MTR_1g097490 24.638 276 181 11 1 266 132 390 1.64e-11 64.3
MS.gene43789.t1 MTR_7g017040 24.667 300 177 13 12 274 112 399 1.64e-11 64.3
MS.gene43789.t1 MTR_5g007610 28.112 249 147 12 8 235 126 363 1.66e-11 64.3
MS.gene43789.t1 MTR_7g078860 30.137 219 123 10 15 213 120 328 1.82e-11 64.3
MS.gene43789.t1 MTR_6g090180 23.723 274 164 8 30 267 170 434 2.10e-11 63.9
MS.gene43789.t1 MTR_4g019990 25.606 289 168 12 15 265 108 387 2.26e-11 63.9
MS.gene43789.t1 MTR_7g066850 24.172 302 172 13 13 269 118 407 2.71e-11 63.9
MS.gene43789.t1 MTR_2g021800 25.279 269 161 10 33 267 150 412 2.73e-11 63.5
MS.gene43789.t1 MTR_3g072470 25.818 275 138 12 15 258 130 369 3.42e-11 63.5
MS.gene43789.t1 MTR_3g009240 27.368 190 119 6 25 206 140 318 3.46e-11 63.2
MS.gene43789.t1 MTR_7g056503 25.442 283 180 12 14 266 116 397 4.18e-11 63.2
MS.gene43789.t1 MTR_3g083640 23.723 274 181 10 2 266 148 402 4.56e-11 63.2
MS.gene43789.t1 MTR_1g097560 27.969 261 142 14 1 242 119 352 4.80e-11 62.8
MS.gene43789.t1 MTR_2g022000 25.506 247 146 9 33 245 149 391 5.10e-11 62.8
MS.gene43789.t1 MTR_2g426020 26.070 257 143 11 17 239 100 343 5.47e-11 62.8
MS.gene43789.t1 MTR_3g010390 26.699 206 129 8 17 205 136 336 6.54e-11 62.4
MS.gene43789.t1 MTR_6g065510 26.829 205 120 9 25 209 67 261 6.69e-11 62.4
MS.gene43789.t1 MTR_8g099460 28.125 224 128 11 8 204 84 301 7.84e-11 62.4
MS.gene43789.t1 MTR_3g030310 25.907 193 121 7 17 200 127 306 8.26e-11 62.0
MS.gene43789.t1 MTR_5g059430 24.345 267 161 13 33 269 169 424 8.28e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43789.t1 AT4G12560 29.386 228 136 10 2 205 78 304 2.73e-14 72.8
MS.gene43789.t1 AT4G12560 29.386 228 136 10 2 205 78 304 2.73e-14 72.8
MS.gene43789.t1 AT4G12560 29.386 228 136 10 2 205 78 304 2.73e-14 72.8
MS.gene43789.t1 AT3G06240 25.837 209 123 8 13 203 163 357 5.43e-13 68.9
MS.gene43789.t1 AT3G23880 28.283 198 124 7 14 202 111 299 2.18e-12 67.0

Find 61 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAATTATTTCTATGGTTT+TGG 0.152964 5.1:+54511331 MS.gene43789:CDS
GTCCTAGAGTGTATGATTTA+TGG 0.242282 5.1:+54511641 MS.gene43789:CDS
GAATCTTTGCTTTCACTTCC+TGG 0.245425 5.1:+54511949 MS.gene43789:CDS
GTTATTCAAGCCTTTATCTA+TGG 0.251337 5.1:+54511245 MS.gene43789:CDS
TTAGAAGCAAATTATTTCTA+TGG 0.288587 5.1:+54511325 MS.gene43789:CDS
TTTATAGATCTTGATATTAG+AGG 0.291130 5.1:+54511193 MS.gene43789:CDS
AATACAACGTGCAATCTTCT+TGG 0.293336 5.1:+54511737 MS.gene43789:CDS
ATAATGGTATAGTTGAAATA+TGG 0.314057 5.1:+54511704 MS.gene43789:CDS
TATGATTTATGGCTATTTAG+AGG 0.334732 5.1:+54511652 MS.gene43789:CDS
CTCAATTTCCTTCCACGTAT+TGG 0.336524 5.1:-54511452 None:intergenic
TAGATAAAGGCTTGAATAAC+CGG 0.360813 5.1:-54511242 None:intergenic
GAAATAATTTGCTTCTAAAT+CGG 0.363111 5.1:-54511320 None:intergenic
ATAGAGTTGCCTTTGCCTAA+TGG 0.368828 5.1:+54511619 MS.gene43789:CDS
AGAGTAGATACGCTCTTAAA+TGG 0.374090 5.1:+54511517 MS.gene43789:CDS
TTGGCCAAGTATAATGATAA+AGG 0.374960 5.1:+54511862 MS.gene43789:CDS
TCTGATATGTACATTGATAA+TGG 0.380566 5.1:+54511688 MS.gene43789:CDS
GTGGTTTGTTATCTCACTTT+GGG 0.388159 5.1:+54511413 MS.gene43789:CDS
AGATCAACGAATGTTATTGT+TGG 0.399345 5.1:+54511571 MS.gene43789:CDS
CTGTCCTTTATCATTATACT+TGG 0.401300 5.1:-54511866 None:intergenic
GGTGGTTTGTTATCTCACTT+TGG 0.409078 5.1:+54511412 MS.gene43789:CDS
TCTTAAATGGTGTTATTCAC+TGG 0.426216 5.1:+54511530 MS.gene43789:CDS
TATCAAGATCTATAAAATAT+CGG 0.426644 5.1:-54511186 None:intergenic
TCCTGGTGGTGACAGTGATC+AGG 0.428106 5.1:+54511966 MS.gene43789:CDS
TTGGATTTCATTTAACAGAA+AGG 0.439356 5.1:+54511590 MS.gene43789:CDS
TGGATGGATTCCATAGATAA+AGG 0.442360 5.1:-54511255 None:intergenic
AAATGGTGTTATTCACTGGT+TGG 0.457182 5.1:+54511534 MS.gene43789:CDS
GGAATCATGAACAGTAGTAA+AGG 0.490153 5.1:-54511299 None:intergenic
GAATCATGAACAGTAGTAAA+GGG 0.494070 5.1:-54511298 None:intergenic
TCCTACTCGGACCATGATGA+TGG 0.505877 5.1:+54511904 MS.gene43789:CDS
CAATACGTGGAAGGAAATTG+AGG 0.520416 5.1:+54511453 MS.gene43789:CDS
TCATACACTCTAGGACCATT+AGG 0.527398 5.1:-54511634 None:intergenic
TGGAACTGACGATAGTGTAT+TGG 0.529716 5.1:+54511843 MS.gene43789:CDS
TAATGGTATAGTTGAAATAT+GGG 0.533072 5.1:+54511705 MS.gene43789:CDS
CATAGCACATTGAAAGAACC+AGG 0.552532 5.1:-54511378 None:intergenic
GCCTGATCACTGTCACCACC+AGG 0.559608 5.1:-54511967 None:intergenic
TGGCTATTTAGAGGATGTCT+CGG 0.559992 5.1:+54511661 MS.gene43789:CDS
TTTGGGCAAAAGTAGTGCAT+GGG 0.560044 5.1:-54511793 None:intergenic
GGGTATTTGTCTGTGAACTC+CGG 0.580241 5.1:-54511278 None:intergenic
GATAACAAACCACCCGCACT+CGG 0.580473 5.1:-54511403 None:intergenic
ATTGAGAGCCAATACGTGGA+AGG 0.584328 5.1:+54511444 MS.gene43789:CDS
TGTCTGTGAACTCCGGTGGA+TGG 0.584561 5.1:-54511271 None:intergenic
ATGTGCTATGATCCGAGTGC+GGG 0.591052 5.1:+54511391 MS.gene43789:CDS
GATATTAGAGGTGAATGTAG+AGG 0.593796 5.1:+54511205 MS.gene43789:CDS
AATACGTGGAAGGAAATTGA+GGG 0.594846 5.1:+54511454 MS.gene43789:CDS
TGAGTCTACTGATGATTACC+TGG 0.607214 5.1:+54511360 MS.gene43789:CDS
TATCTATGGAATCCATCCAC+CGG 0.612787 5.1:+54511259 MS.gene43789:CDS
AGCCATAAATCATACACTCT+AGG 0.618217 5.1:-54511643 None:intergenic
CCACTAGGGATCCATCATCA+TGG 0.620597 5.1:-54511915 None:intergenic
GCTAGAGCATCACTCCTACT+CGG 0.623312 5.1:+54511891 MS.gene43789:CDS
TGCTATGATCCGAGTGCGGG+TGG 0.623842 5.1:+54511394 MS.gene43789:CDS
TCTTTGCTTTCACTTCCTGG+TGG 0.625428 5.1:+54511952 MS.gene43789:CDS
ATTCTGTATACATAACCACT+AGG 0.630116 5.1:-54511930 None:intergenic
TTCTGTATACATAACCACTA+GGG 0.637981 5.1:-54511929 None:intergenic
TATTTGTCTGTGAACTCCGG+TGG 0.653787 5.1:-54511275 None:intergenic
AATGTGCTATGATCCGAGTG+CGG 0.661055 5.1:+54511390 MS.gene43789:CDS
TCCATCATCATGGTCCGAGT+AGG 0.664813 5.1:-54511905 None:intergenic
ACTCTAGGACCATTAGGCAA+AGG 0.668741 5.1:-54511628 None:intergenic
CCATGATGATGGATCCCTAG+TGG 0.673107 5.1:+54511915 MS.gene43789:CDS
TTTCATTGAGAGCCAATACG+TGG 0.680917 5.1:+54511440 MS.gene43789:CDS
ACGTGGAAGGAAATTGAGGG+TGG 0.704970 5.1:+54511457 MS.gene43789:CDS
AGATCTATAAAATATCGGTG+AGG 0.738556 5.1:-54511181 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCAAGATCTATAAAATAT+CGG - chr5.1:54511189-54511208 None:intergenic 15.0%
!! ATAATGGTATAGTTGAAATA+TGG + chr5.1:54511704-54511723 MS.gene43789:CDS 20.0%
!! TAATGGTATAGTTGAAATAT+GGG + chr5.1:54511705-54511724 MS.gene43789:CDS 20.0%
!! TTTATAGATCTTGATATTAG+AGG + chr5.1:54511193-54511212 MS.gene43789:CDS 20.0%
!!! CAAATTATTTCTATGGTTTT+GGG + chr5.1:54511332-54511351 MS.gene43789:CDS 20.0%
!!! CACTTTTTGTACAATATTTT+GGG - chr5.1:54511813-54511832 None:intergenic 20.0%
!!! GAAATAATTTGCTTCTAAAT+CGG - chr5.1:54511323-54511342 None:intergenic 20.0%
!!! TTAGAAGCAAATTATTTCTA+TGG + chr5.1:54511325-54511344 MS.gene43789:CDS 20.0%
! CCAAAATATTGTACAAAAAG+TGG + chr5.1:54511811-54511830 MS.gene43789:CDS 25.0%
! TATGATTTATGGCTATTTAG+AGG + chr5.1:54511652-54511671 MS.gene43789:CDS 25.0%
! TCTGATATGTACATTGATAA+TGG + chr5.1:54511688-54511707 MS.gene43789:CDS 25.0%
! TTGGATTTCATTTAACAGAA+AGG + chr5.1:54511590-54511609 MS.gene43789:CDS 25.0%
!!! CCACTTTTTGTACAATATTT+TGG - chr5.1:54511814-54511833 None:intergenic 25.0%
!!! GCAAATTATTTCTATGGTTT+TGG + chr5.1:54511331-54511350 MS.gene43789:CDS 25.0%
AGATCAACGAATGTTATTGT+TGG + chr5.1:54511571-54511590 MS.gene43789:CDS 30.0%
AGATCTATAAAATATCGGTG+AGG - chr5.1:54511184-54511203 None:intergenic 30.0%
ATTCTGTATACATAACCACT+AGG - chr5.1:54511933-54511952 None:intergenic 30.0%
CTGTCCTTTATCATTATACT+TGG - chr5.1:54511869-54511888 None:intergenic 30.0%
GAATCATGAACAGTAGTAAA+GGG - chr5.1:54511301-54511320 None:intergenic 30.0%
GTTATTCAAGCCTTTATCTA+TGG + chr5.1:54511245-54511264 MS.gene43789:CDS 30.0%
TAGATAAAGGCTTGAATAAC+CGG - chr5.1:54511245-54511264 None:intergenic 30.0%
TTCTGTATACATAACCACTA+GGG - chr5.1:54511932-54511951 None:intergenic 30.0%
TTGGCCAAGTATAATGATAA+AGG + chr5.1:54511862-54511881 MS.gene43789:CDS 30.0%
! ACTCTTGTTCTTTCTTTTGA+TGG + chr5.1:54511766-54511785 MS.gene43789:CDS 30.0%
!! TCTTAAATGGTGTTATTCAC+TGG + chr5.1:54511530-54511549 MS.gene43789:CDS 30.0%
AATACAACGTGCAATCTTCT+TGG + chr5.1:54511737-54511756 MS.gene43789:CDS 35.0%
AATACGTGGAAGGAAATTGA+GGG + chr5.1:54511454-54511473 MS.gene43789:CDS 35.0%
ACAAAAAGTGGTGATATCGT+TGG + chr5.1:54511823-54511842 MS.gene43789:CDS 35.0%
AGAGTAGATACGCTCTTAAA+TGG + chr5.1:54511517-54511536 MS.gene43789:CDS 35.0%
AGCCATAAATCATACACTCT+AGG - chr5.1:54511646-54511665 None:intergenic 35.0%
GATATTAGAGGTGAATGTAG+AGG + chr5.1:54511205-54511224 MS.gene43789:CDS 35.0%
GGAATCATGAACAGTAGTAA+AGG - chr5.1:54511302-54511321 None:intergenic 35.0%
GTCCTAGAGTGTATGATTTA+TGG + chr5.1:54511641-54511660 MS.gene43789:CDS 35.0%
! TGGATGGATTCCATAGATAA+AGG - chr5.1:54511258-54511277 None:intergenic 35.0%
!! AAATGGTGTTATTCACTGGT+TGG + chr5.1:54511534-54511553 MS.gene43789:CDS 35.0%
!! AGAGGCTTTATACTTTTCTC+CGG + chr5.1:54511223-54511242 MS.gene43789:CDS 35.0%
!! GTGGTTTGTTATCTCACTTT+GGG + chr5.1:54511413-54511432 MS.gene43789:CDS 35.0%
ATAGAGTTGCCTTTGCCTAA+TGG + chr5.1:54511619-54511638 MS.gene43789:CDS 40.0%
CAATACGTGGAAGGAAATTG+AGG + chr5.1:54511453-54511472 MS.gene43789:CDS 40.0%
CATAGCACATTGAAAGAACC+AGG - chr5.1:54511381-54511400 None:intergenic 40.0%
CTCAATTTCCTTCCACGTAT+TGG - chr5.1:54511455-54511474 None:intergenic 40.0%
GAATCTTTGCTTTCACTTCC+TGG + chr5.1:54511949-54511968 MS.gene43789:CDS 40.0%
TATCTATGGAATCCATCCAC+CGG + chr5.1:54511259-54511278 MS.gene43789:CDS 40.0%
TGAGTCTACTGATGATTACC+TGG + chr5.1:54511360-54511379 MS.gene43789:CDS 40.0%
TGGAACTGACGATAGTGTAT+TGG + chr5.1:54511843-54511862 MS.gene43789:CDS 40.0%
TGGCTATTTAGAGGATGTCT+CGG + chr5.1:54511661-54511680 MS.gene43789:CDS 40.0%
TTTCATTGAGAGCCAATACG+TGG + chr5.1:54511440-54511459 MS.gene43789:CDS 40.0%
TTTGGGCAAAAGTAGTGCAT+GGG - chr5.1:54511796-54511815 None:intergenic 40.0%
! TTTTGGGCAAAAGTAGTGCA+TGG - chr5.1:54511797-54511816 None:intergenic 40.0%
!! GGTGGTTTGTTATCTCACTT+TGG + chr5.1:54511412-54511431 MS.gene43789:CDS 40.0%
!! TCATACACTCTAGGACCATT+AGG - chr5.1:54511637-54511656 None:intergenic 40.0%
AATGTGCTATGATCCGAGTG+CGG + chr5.1:54511390-54511409 MS.gene43789:CDS 45.0%
ATTGAGAGCCAATACGTGGA+AGG + chr5.1:54511444-54511463 MS.gene43789:CDS 45.0%
GGGTATTTGTCTGTGAACTC+CGG - chr5.1:54511281-54511300 None:intergenic 45.0%
TATTTGTCTGTGAACTCCGG+TGG - chr5.1:54511278-54511297 None:intergenic 45.0%
TCTTTGCTTTCACTTCCTGG+TGG + chr5.1:54511952-54511971 MS.gene43789:CDS 45.0%
!! ACTCTAGGACCATTAGGCAA+AGG - chr5.1:54511631-54511650 None:intergenic 45.0%
ACGTGGAAGGAAATTGAGGG+TGG + chr5.1:54511457-54511476 MS.gene43789:CDS 50.0%
ATGTGCTATGATCCGAGTGC+GGG + chr5.1:54511391-54511410 MS.gene43789:CDS 50.0%
CCACTAGGGATCCATCATCA+TGG - chr5.1:54511918-54511937 None:intergenic 50.0%
GATAACAAACCACCCGCACT+CGG - chr5.1:54511406-54511425 None:intergenic 50.0%
TCCATCATCATGGTCCGAGT+AGG - chr5.1:54511908-54511927 None:intergenic 50.0%
TCCTACTCGGACCATGATGA+TGG + chr5.1:54511904-54511923 MS.gene43789:CDS 50.0%
! GCTAGAGCATCACTCCTACT+CGG + chr5.1:54511891-54511910 MS.gene43789:CDS 50.0%
!! CCATGATGATGGATCCCTAG+TGG + chr5.1:54511915-54511934 MS.gene43789:CDS 50.0%
TGTCTGTGAACTCCGGTGGA+TGG - chr5.1:54511274-54511293 None:intergenic 55.0%
! TCCTGGTGGTGACAGTGATC+AGG + chr5.1:54511966-54511985 MS.gene43789:CDS 55.0%
GCCTGATCACTGTCACCACC+AGG - chr5.1:54511970-54511989 None:intergenic 60.0%
TGCTATGATCCGAGTGCGGG+TGG + chr5.1:54511394-54511413 MS.gene43789:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 54511166 54511993 54511166 ID=MS.gene43789
chr5.1 mRNA 54511166 54511993 54511166 ID=MS.gene43789.t1;Parent=MS.gene43789
chr5.1 exon 54511166 54511993 54511166 ID=MS.gene43789.t1.exon1;Parent=MS.gene43789.t1
chr5.1 CDS 54511166 54511993 54511166 ID=cds.MS.gene43789.t1;Parent=MS.gene43789.t1
Gene Sequence

>MS.gene43789

ATGGATTTTTCATCTCCTCACCGATATTTTATAGATCTTGATATTAGAGGTGAATGTAGAGGCTTTATACTTTTCTCCGGTTATTCAAGCCTTTATCTATGGAATCCATCCACCGGAGTTCACAGACAAATACCCTTTACTACTGTTCATGATTCCGATTTAGAAGCAAATTATTTCTATGGTTTTGGGTATGATGAGTCTACTGATGATTACCTGGTTCTTTCAATGTGCTATGATCCGAGTGCGGGTGGTTTGTTATCTCACTTTGGGCTTTTTTCATTGAGAGCCAATACGTGGAAGGAAATTGAGGGTGGTGACAATTTGCGTTATTCACAGTCGTGTATGTATTCTAGAGTAGATACGCTCTTAAATGGTGTTATTCACTGGTTGGCTTTTCGTAATGATAGATCAACGAATGTTATTGTTGGATTTCATTTAACAGAAAGGAAACTTATAGAGTTGCCTTTGCCTAATGGTCCTAGAGTGTATGATTTATGGCTATTTAGAGGATGTCTCGGTCTATCTGATATGTACATTGATAATGGTATAGTTGAAATATGGGTGATGAAAAAATACAACGTGCAATCTTCTTGGACTAAGACTCTTGTTCTTTCTTTTGATGGCATTCCCATGCACTACTTTTGCCCAAAATATTGTACAAAAAGTGGTGATATCGTTGGAACTGACGATAGTGTATTGGCCAAGTATAATGATAAAGGACAGCTGCTAGAGCATCACTCCTACTCGGACCATGATGATGGATCCCTAGTGGTTATGTATACAGAATCTTTGCTTTCACTTCCTGGTGGTGACAGTGATCAGGCTTAA
Protein sequence

>MS.gene43789.t1

MDFSSPHRYFIDLDIRGECRGFILFSGYSSLYLWNPSTGVHRQIPFTTVHDSDLEANYFYGFGYDESTDDYLVLSMCYDPSAGGLLSHFGLFSLRANTWKEIEGGDNLRYSQSCMYSRVDTLLNGVIHWLAFRNDRSTNVIVGFHLTERKLIELPLPNGPRVYDLWLFRGCLGLSDMYIDNGIVEIWVMKKYNVQSSWTKTLVLSFDGIPMHYFCPKYCTKSGDIVGTDDSVLAKYNDKGQLLEHHSYSDHDDGSLVVMYTESLLSLPGGDSDQA