AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85043


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85043.t1 MTR_4g023380 77.000 200 27 1 2 182 180 379 2.80e-108 314
MS.gene85043.t1 MTR_4g023090 76.804 194 30 2 2 182 195 386 2.69e-101 297
MS.gene85043.t1 MTR_4g023130 73.481 181 41 4 2 181 138 312 3.65e-87 258
MS.gene85043.t1 MTR_4g023080 72.928 181 42 4 2 181 179 353 7.77e-85 254
MS.gene85043.t1 MTR_4g021785 66.484 182 53 4 2 181 179 354 9.93e-76 231
MS.gene85043.t1 MTR_4g023070 63.542 192 54 3 2 181 182 369 3.19e-75 230
MS.gene85043.t1 MTR_4g023120 61.140 193 59 3 2 182 182 370 2.09e-72 223
MS.gene85043.t1 MTR_4g116320 62.360 178 57 5 2 174 80 252 3.32e-65 201
MS.gene85043.t1 MTR_3g103020 60.221 181 64 5 4 181 202 377 8.56e-65 203
MS.gene85043.t1 MTR_6g060650 57.377 183 68 5 2 181 197 372 1.42e-64 203
MS.gene85043.t1 MTR_4g023150 61.538 182 63 5 2 180 181 358 1.13e-63 200
MS.gene85043.t1 MTR_4g023200 60.440 182 65 5 2 180 181 358 1.33e-62 197
MS.gene85043.t1 MTR_4g023440 58.564 181 33 3 2 182 194 332 5.14e-62 195
MS.gene85043.t1 MTR_4g023190 60.989 182 63 6 2 180 181 357 1.31e-61 195
MS.gene85043.t1 MTR_4g116330 57.297 185 69 5 2 181 200 379 1.07e-59 191
MS.gene85043.t1 MTR_4g117720 57.143 189 66 6 2 181 195 377 6.46e-59 188
MS.gene85043.t1 MTR_4g109270 59.639 166 60 4 18 181 203 363 3.05e-58 186
MS.gene85043.t1 MTR_4g109280 53.763 186 72 6 2 181 181 358 6.38e-57 182
MS.gene85043.t1 MTR_8g040935 57.647 170 61 5 18 181 43 207 6.27e-56 176
MS.gene85043.t1 MTR_3g034180 58.788 165 58 5 20 182 333 489 8.31e-55 181
MS.gene85043.t1 MTR_8g433030 57.386 176 67 6 3 174 165 336 2.21e-54 176
MS.gene85043.t1 MTR_8g032390 57.386 176 67 6 3 174 198 369 7.31e-54 175
MS.gene85043.t1 MTR_4g117770 55.978 184 73 5 3 182 188 367 1.09e-52 172
MS.gene85043.t1 MTR_4g117810 55.978 184 73 5 3 182 188 367 1.09e-52 172
MS.gene85043.t1 MTR_4g094365 54.645 183 74 6 2 179 185 363 3.53e-52 171
MS.gene85043.t1 MTR_6g463430 52.432 185 75 5 2 182 27 202 4.75e-52 165
MS.gene85043.t1 MTR_4g117850 54.301 186 71 6 2 179 197 376 1.03e-51 170
MS.gene85043.t1 MTR_4g122850 54.098 183 73 7 5 181 188 365 1.22e-51 170
MS.gene85043.t1 MTR_3g034160 55.367 177 69 5 9 182 206 375 8.18e-51 167
MS.gene85043.t1 MTR_4g117780 54.348 184 76 5 3 182 188 367 3.54e-50 166
MS.gene85043.t1 MTR_8g433040 55.152 165 58 5 18 182 216 364 1.85e-49 164
MS.gene85043.t1 MTR_8g032380 55.152 165 58 5 18 182 216 364 1.85e-49 164
MS.gene85043.t1 MTR_5g070130 54.938 162 68 3 22 181 218 376 2.12e-49 164
MS.gene85043.t1 MTR_3g034130 54.140 157 65 3 7 161 204 355 2.72e-49 163
MS.gene85043.t1 MTR_4g118380 53.374 163 63 3 2 155 199 357 8.93e-49 162
MS.gene85043.t1 MTR_1g055175 48.352 182 77 7 4 181 32 200 1.94e-47 154
MS.gene85043.t1 MTR_5g070088 53.374 163 71 3 22 182 227 386 3.30e-46 155
MS.gene85043.t1 MTR_1g055195 53.846 156 63 4 22 174 323 472 5.87e-46 157
MS.gene85043.t1 MTR_5g097210 55.280 161 62 5 17 174 204 357 1.13e-45 154
MS.gene85043.t1 MTR_1g055165 48.619 181 76 7 5 181 150 317 1.91e-45 152
MS.gene85043.t1 MTR_4g118400 48.295 176 71 3 2 174 136 294 2.76e-43 146
MS.gene85043.t1 MTR_1g055135 47.458 177 77 5 4 174 203 369 3.71e-43 147
MS.gene85043.t1 MTR_5g070080 51.892 185 78 7 2 181 207 385 6.94e-43 147
MS.gene85043.t1 MTR_4g117870 58.955 134 46 5 51 182 141 267 9.57e-43 144
MS.gene85043.t1 MTR_1g055250 47.945 146 69 4 22 167 115 253 1.39e-37 132
MS.gene85043.t1 MTR_4g116330 45.405 185 52 5 2 181 200 340 5.09e-37 130
MS.gene85043.t1 MTR_7g079370 44.505 182 85 6 2 174 176 350 2.03e-36 129
MS.gene85043.t1 MTR_4g117750 47.849 186 78 7 2 174 174 353 1.12e-35 130
MS.gene85043.t1 MTR_4g117750 51.852 135 52 4 54 178 350 481 1.24e-29 113
MS.gene85043.t1 MTR_5g023000 64.646 99 31 2 85 181 2 98 1.57e-35 120
MS.gene85043.t1 MTR_4g118370 45.000 180 68 8 2 174 99 254 1.74e-35 125
MS.gene85043.t1 MTR_4g118360 54.887 133 47 6 54 181 1 125 2.24e-35 120
MS.gene85043.t1 MTR_4g117530 42.541 181 67 5 2 181 108 252 4.01e-32 116
MS.gene85043.t1 MTR_7g079400 45.294 170 77 6 18 178 173 335 5.40e-31 115
MS.gene85043.t1 MTR_4g023430 66.265 83 21 2 105 182 142 222 3.33e-29 107
MS.gene85043.t1 MTR_4g121580 42.442 172 63 7 3 170 200 339 4.62e-29 110
MS.gene85043.t1 MTR_8g102520 41.975 162 82 5 18 174 162 316 1.87e-28 108
MS.gene85043.t1 MTR_7g079420 43.195 169 79 7 18 178 983 1142 5.77e-27 107
MS.gene85043.t1 MTR_4g122860 42.373 177 66 7 5 174 167 314 1.54e-26 103
MS.gene85043.t1 MTR_7g079450 45.652 138 63 4 40 174 3 131 3.02e-26 97.8
MS.gene85043.t1 MTR_8g098830 40.625 160 83 6 18 175 67 216 7.03e-26 99.4
MS.gene85043.t1 MTR_4g114700 52.294 109 46 2 80 182 122 230 1.79e-23 93.2
MS.gene85043.t1 MTR_4g023100 76.271 59 12 1 124 182 63 119 6.53e-23 88.6
MS.gene85043.t1 MTR_7g117720 60.465 86 28 2 95 174 186 271 1.47e-21 89.0
MS.gene85043.t1 MTR_2g048580 35.404 161 86 5 18 174 190 336 4.27e-20 85.9
MS.gene85043.t1 MTR_4g122840 39.416 137 44 4 45 179 164 263 1.87e-17 77.8
MS.gene85043.t1 MTR_4g117430 50.617 81 39 1 2 82 76 155 3.18e-17 75.1
MS.gene85043.t1 MTR_8g013005 60.345 58 19 2 126 181 3 58 9.75e-14 63.2
MS.gene85043.t1 MTR_1g055145 48.052 77 35 2 22 98 131 202 2.97e-13 66.6
MS.gene85043.t1 MTR_4g109290 60.714 56 18 2 128 181 134 187 1.66e-12 63.2
MS.gene85043.t1 MTR_8g013010 46.341 82 32 3 2 79 236 309 9.53e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGTTGCCTTTGATTTAA+TGG 0.113056 4.2:+67733640 MS.gene85043:CDS
CATTCTGGTTTGTGGGTATT+TGG 0.171406 4.2:+67733719 MS.gene85043:CDS
AGAAAAGGGTTGCTATTTAA+TGG 0.226317 4.2:+67733572 MS.gene85043:CDS
TTGGAGAATTTCTCAGTATA+TGG 0.239627 4.2:+67733738 MS.gene85043:CDS
TTGGCTTTGCGTCGTAATTT+AGG 0.260116 4.2:+67733608 MS.gene85043:CDS
ATGATAAAGGACAGTTATTA+AGG 0.300794 4.2:+67733954 MS.gene85043:CDS
GAAAAGGGTTGCTATTTAAT+GGG 0.312663 4.2:+67733573 MS.gene85043:CDS
ATAATGATACAGTTGAAATA+TGG 0.320851 4.2:+67733780 MS.gene85043:CDS
CACAAAGCATTGGTACATTC+TGG 0.326889 4.2:+67733704 MS.gene85043:CDS
TATTTAATGGGGCTATTCAT+TGG 0.360740 4.2:+67733585 MS.gene85043:CDS
GCTTTGTGGTCAACATCATT+TGG 0.369346 4.2:-67733689 None:intergenic
GTCATCCCTTCTCGCAGAAA+AGG 0.395590 4.2:+67733557 MS.gene85043:CDS
TGGAGAATTTCTCAGTATAT+GGG 0.402920 4.2:+67733739 MS.gene85043:CDS
CATTGGTACATTCTGGTTTG+TGG 0.418702 4.2:+67733711 MS.gene85043:CDS
ATTGGTACATTCTGGTTTGT+GGG 0.430276 4.2:+67733712 MS.gene85043:CDS
CGAGAAGGGATGACAACATA+AGG 0.430786 4.2:-67733548 None:intergenic
TAATGGGGCTATTCATTGGT+TGG 0.433983 4.2:+67733589 MS.gene85043:CDS
AATACAAAGTGCATTCATCT+TGG 0.438377 4.2:+67733813 MS.gene85043:CDS
TGCTTGTTGTCACCAGGGAC+AGG 0.438679 4.2:-67734040 None:intergenic
CTGCTTTCACTCCCTGTCCC+TGG 0.445586 4.2:+67734028 MS.gene85043:CDS
ATCCGAAGTTGTCATGTATA+CGG 0.460650 4.2:+67734000 MS.gene85043:CDS
TTTCCTTTCCATTAAATCAA+AGG 0.474723 4.2:-67733648 None:intergenic
TAAATTTGCTTGTTGTCACC+AGG 0.478700 4.2:-67734046 None:intergenic
CAGAATGTACCAATGCTTTG+TGG 0.480428 4.2:-67733703 None:intergenic
TTGGTGAAGTATAATGATAA+AGG 0.492510 4.2:+67733941 MS.gene85043:CDS
AAGAAATCGTGGTACTAGAT+TGG 0.508666 4.2:+67733922 MS.gene85043:CDS
TCATCCCTTCTCGCAGAAAA+GGG 0.509731 4.2:+67733558 MS.gene85043:CDS
ATTTCTCAGTATATGGGCCA+TGG 0.520677 4.2:+67733745 MS.gene85043:CDS
TGGTCAACATCATTTGGAAG+AGG 0.521206 4.2:-67733683 None:intergenic
TAATGATACAGTTGAAATAT+GGG 0.533072 4.2:+67733781 MS.gene85043:CDS
TTGCCTTTGATTTAATGGAA+AGG 0.533360 4.2:+67733645 MS.gene85043:CDS
TTGAAAGTAATGATCATGAA+TGG 0.538193 4.2:-67733864 None:intergenic
GCTTGTTGTCACCAGGGACA+GGG 0.550530 4.2:-67734039 None:intergenic
ATTATCATTATCATAATCCA+TGG 0.571255 4.2:-67733762 None:intergenic
AGGGATGACAACATAAGGGA+AGG 0.583508 4.2:-67733543 None:intergenic
TGATGTTGACCACAAAGCAT+TGG 0.583728 4.2:+67733694 MS.gene85043:CDS
GAGAAGGGATGACAACATAA+GGG 0.592203 4.2:-67733547 None:intergenic
GGGATGACAACATAAGGGAA+GGG 0.603054 4.2:-67733542 None:intergenic
AAAAGGGTTGCTATTTAATG+GGG 0.619929 4.2:+67733574 MS.gene85043:CDS
AAATTTGCTTGTTGTCACCA+GGG 0.627879 4.2:-67734045 None:intergenic
ATAATACATGAAAGAAACTG+AGG 0.634313 4.2:+67733514 None:intergenic
CTCCGTATACATGACAACTT+CGG 0.636118 4.2:-67734002 None:intergenic
GTATACATGACAACTTCGGA+TGG 0.641195 4.2:-67733998 None:intergenic
TTCGGATGGACTATTACAGA+AGG 0.644435 4.2:-67733984 None:intergenic
GATATTGTTGGAAGAAATCG+TGG 0.669142 4.2:+67733911 MS.gene85043:CDS
TAATACATGAAAGAAACTGA+GGG 0.684928 4.2:+67733515 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATGATACAGTTGAAATA+TGG + chr4.2:67733780-67733799 MS.gene85043:CDS 20.0%
!! ATTATCATTATCATAATCCA+TGG - chr4.2:67733765-67733784 None:intergenic 20.0%
!! TAATGATACAGTTGAAATAT+GGG + chr4.2:67733781-67733800 MS.gene85043:CDS 20.0%
! ATGATAAAGGACAGTTATTA+AGG + chr4.2:67733954-67733973 MS.gene85043:CDS 25.0%
! CCTATACATTCTACAAAAAT+TGG + chr4.2:67733887-67733906 MS.gene85043:CDS 25.0%
! TTGAAAGTAATGATCATGAA+TGG - chr4.2:67733867-67733886 None:intergenic 25.0%
! TTGGTGAAGTATAATGATAA+AGG + chr4.2:67733941-67733960 MS.gene85043:CDS 25.0%
! TTTCCTTTCCATTAAATCAA+AGG - chr4.2:67733651-67733670 None:intergenic 25.0%
!! ACAAAAATTGGTGATATTGT+TGG + chr4.2:67733899-67733918 MS.gene85043:CDS 25.0%
!! CCAATTTTTGTAGAATGTAT+AGG - chr4.2:67733890-67733909 None:intergenic 25.0%
AATACAAAGTGCATTCATCT+TGG + chr4.2:67733813-67733832 MS.gene85043:CDS 30.0%
TATTTAATGGGGCTATTCAT+TGG + chr4.2:67733585-67733604 MS.gene85043:CDS 30.0%
TGGAGAATTTCTCAGTATAT+GGG + chr4.2:67733739-67733758 MS.gene85043:CDS 30.0%
TTGGAGAATTTCTCAGTATA+TGG + chr4.2:67733738-67733757 MS.gene85043:CDS 30.0%
! CATTGTTGCCTTTGATTTAA+TGG + chr4.2:67733640-67733659 MS.gene85043:CDS 30.0%
! TTGCCTTTGATTTAATGGAA+AGG + chr4.2:67733645-67733664 MS.gene85043:CDS 30.0%
!! AAAAGGGTTGCTATTTAATG+GGG + chr4.2:67733574-67733593 MS.gene85043:CDS 30.0%
!! AGAAAAGGGTTGCTATTTAA+TGG + chr4.2:67733572-67733591 MS.gene85043:CDS 30.0%
!! GAAAAGGGTTGCTATTTAAT+GGG + chr4.2:67733573-67733592 MS.gene85043:CDS 30.0%
AAGAAATCGTGGTACTAGAT+TGG + chr4.2:67733922-67733941 MS.gene85043:CDS 35.0%
ATCCGAAGTTGTCATGTATA+CGG + chr4.2:67734000-67734019 MS.gene85043:CDS 35.0%
GATATTGTTGGAAGAAATCG+TGG + chr4.2:67733911-67733930 MS.gene85043:CDS 35.0%
!! AAATTTGCTTGTTGTCACCA+GGG - chr4.2:67734048-67734067 None:intergenic 35.0%
!! ATTGGTACATTCTGGTTTGT+GGG + chr4.2:67733712-67733731 MS.gene85043:CDS 35.0%
!! TAAATTTGCTTGTTGTCACC+AGG - chr4.2:67734049-67734068 None:intergenic 35.0%
ATTTCTCAGTATATGGGCCA+TGG + chr4.2:67733745-67733764 MS.gene85043:CDS 40.0%
CAGAATGTACCAATGCTTTG+TGG - chr4.2:67733706-67733725 None:intergenic 40.0%
CTCCGTATACATGACAACTT+CGG - chr4.2:67734005-67734024 None:intergenic 40.0%
GAGAAGGGATGACAACATAA+GGG - chr4.2:67733550-67733569 None:intergenic 40.0%
GCTTTGTGGTCAACATCATT+TGG - chr4.2:67733692-67733711 None:intergenic 40.0%
GTATACATGACAACTTCGGA+TGG - chr4.2:67734001-67734020 None:intergenic 40.0%
TAATGGGGCTATTCATTGGT+TGG + chr4.2:67733589-67733608 MS.gene85043:CDS 40.0%
TGATGTTGACCACAAAGCAT+TGG + chr4.2:67733694-67733713 MS.gene85043:CDS 40.0%
TGGTCAACATCATTTGGAAG+AGG - chr4.2:67733686-67733705 None:intergenic 40.0%
!! CACAAAGCATTGGTACATTC+TGG + chr4.2:67733704-67733723 MS.gene85043:CDS 40.0%
!! CATTCTGGTTTGTGGGTATT+TGG + chr4.2:67733719-67733738 MS.gene85043:CDS 40.0%
!! CATTGGTACATTCTGGTTTG+TGG + chr4.2:67733711-67733730 MS.gene85043:CDS 40.0%
!! TTCGGATGGACTATTACAGA+AGG - chr4.2:67733987-67734006 None:intergenic 40.0%
!! TTGGCTTTGCGTCGTAATTT+AGG + chr4.2:67733608-67733627 MS.gene85043:CDS 40.0%
AGGGATGACAACATAAGGGA+AGG - chr4.2:67733546-67733565 None:intergenic 45.0%
CGAGAAGGGATGACAACATA+AGG - chr4.2:67733551-67733570 None:intergenic 45.0%
GGGATGACAACATAAGGGAA+GGG - chr4.2:67733545-67733564 None:intergenic 45.0%
TCATCCCTTCTCGCAGAAAA+GGG + chr4.2:67733558-67733577 MS.gene85043:CDS 45.0%
GTCATCCCTTCTCGCAGAAA+AGG + chr4.2:67733557-67733576 MS.gene85043:CDS 50.0%
! AGCAACCCTTTTCTGCGAGA+AGG - chr4.2:67733566-67733585 None:intergenic 50.0%
! GCAACCCTTTTCTGCGAGAA+GGG - chr4.2:67733565-67733584 None:intergenic 50.0%
!! GCTTGTTGTCACCAGGGACA+GGG - chr4.2:67734042-67734061 None:intergenic 55.0%
!! TGCTTGTTGTCACCAGGGAC+AGG - chr4.2:67734043-67734062 None:intergenic 55.0%
CTGCTTTCACTCCCTGTCCC+TGG + chr4.2:67734028-67734047 MS.gene85043:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 67733521 67734069 67733521 ID=MS.gene85043
chr4.2 mRNA 67733521 67734069 67733521 ID=MS.gene85043.t1;Parent=MS.gene85043
chr4.2 exon 67733521 67734069 67733521 ID=MS.gene85043.t1.exon1;Parent=MS.gene85043.t1
chr4.2 CDS 67733521 67734069 67733521 ID=cds.MS.gene85043.t1;Parent=MS.gene85043.t1
Gene Sequence

>MS.gene85043

ATGAAAGAAACTGAGGGCACTCCCTTCCCTTATGTTGTCATCCCTTCTCGCAGAAAAGGGTTGCTATTTAATGGGGCTATTCATTGGTTGGCTTTGCGTCGTAATTTAGGTTTGATTGTCATTGTTGCCTTTGATTTAATGGAAAGGAAATTATTTGAGATGCCTCTTCCAAATGATGTTGACCACAAAGCATTGGTACATTCTGGTTTGTGGGTATTTGGAGAATTTCTCAGTATATGGGCCATGGATTATGATAATGATAATGATACAGTTGAAATATGGGTGATGAAAGAATACAAAGTGCATTCATCTTGGACTAAAACTCTTATTCTTTCTGTTGATGCCATTCATGATCATTACTTTCAACCTATACATTCTACAAAAATTGGTGATATTGTTGGAAGAAATCGTGGTACTAGATTGGTGAAGTATAATGATAAAGGACAGTTATTAAGGCATTGCTCCTTCTGTAATAGTCCATCCGAAGTTGTCATGTATACGGAGTCTCTGCTTTCACTCCCTGTCCCTGGTGACAACAAGCAAATTTAA

Protein sequence

>MS.gene85043.t1

MKETEGTPFPYVVIPSRRKGLLFNGAIHWLALRRNLGLIVIVAFDLMERKLFEMPLPNDVDHKALVHSGLWVFGEFLSIWAMDYDNDNDTVEIWVMKEYKVHSSWTKTLILSVDAIHDHYFQPIHSTKIGDIVGRNRGTRLVKYNDKGQLLRHCSFCNSPSEVVMYTESLLSLPVPGDNKQI