AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81242

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81242.t1 MTR_5g097210 90.705 312 29 0 1 312 63 374 0.0 584
MS.gene81242.t1 MTR_1g055135 58.947 285 108 6 21 297 88 371 6.62e-108 319
MS.gene81242.t1 MTR_4g117750 57.377 305 117 4 1 297 56 355 2.44e-107 321
MS.gene81242.t1 MTR_4g117750 57.407 108 43 2 194 298 373 480 4.74e-30 119
MS.gene81242.t1 MTR_3g103020 58.766 308 114 6 1 298 71 375 2.50e-106 315
MS.gene81242.t1 MTR_1g055195 58.571 280 98 5 25 297 206 474 8.74e-100 302
MS.gene81242.t1 MTR_4g117720 55.627 311 117 10 1 296 68 372 3.50e-99 296
MS.gene81242.t1 MTR_4g109280 58.086 303 116 8 1 297 58 355 2.68e-97 291
MS.gene81242.t1 MTR_6g060650 53.312 317 123 7 1 298 60 370 5.95e-96 288
MS.gene81242.t1 MTR_4g117850 55.782 294 117 8 9 294 85 373 5.08e-95 286
MS.gene81242.t1 MTR_5g070130 50.915 328 137 10 1 309 63 385 6.82e-95 286
MS.gene81242.t1 MTR_3g034130 51.678 298 128 6 5 287 66 362 2.27e-94 284
MS.gene81242.t1 MTR_4g118370 60.905 243 76 3 57 298 33 257 2.29e-94 280
MS.gene81242.t1 MTR_3g034180 50.962 312 136 7 1 297 176 485 1.99e-91 280
MS.gene81242.t1 MTR_4g122850 50.158 317 145 7 7 312 61 375 1.75e-89 272
MS.gene81242.t1 MTR_3g034160 52.597 308 129 7 5 297 66 371 1.28e-88 270
MS.gene81242.t1 MTR_4g109270 52.427 309 125 8 1 297 62 360 5.59e-88 268
MS.gene81242.t1 MTR_5g070088 48.160 326 133 9 1 295 59 379 1.00e-83 258
MS.gene81242.t1 MTR_4g094365 50.974 308 126 7 8 301 68 364 1.02e-83 257
MS.gene81242.t1 MTR_5g070080 48.000 325 134 9 1 296 62 380 2.54e-82 254
MS.gene81242.t1 MTR_4g117770 50.633 316 130 10 1 301 63 367 2.66e-82 253
MS.gene81242.t1 MTR_4g117810 50.633 316 130 10 1 301 63 367 2.66e-82 253
MS.gene81242.t1 MTR_4g023080 50.329 304 129 10 1 297 62 350 1.44e-78 243
MS.gene81242.t1 MTR_4g117870 48.000 300 110 7 5 297 3 263 3.10e-78 239
MS.gene81242.t1 MTR_4g117780 48.734 316 135 10 1 301 64 367 7.23e-78 242
MS.gene81242.t1 MTR_1g055165 45.246 305 107 7 1 298 64 315 8.21e-76 235
MS.gene81242.t1 MTR_4g118380 50.000 304 116 10 1 274 60 357 2.20e-75 235
MS.gene81242.t1 MTR_8g433040 48.220 309 132 8 5 301 72 364 4.52e-74 232
MS.gene81242.t1 MTR_8g032380 48.220 309 132 8 5 301 72 364 4.52e-74 232
MS.gene81242.t1 MTR_4g023070 47.468 316 134 12 4 297 61 366 7.65e-74 232
MS.gene81242.t1 MTR_4g021785 48.822 297 129 11 9 297 70 351 1.21e-73 231
MS.gene81242.t1 MTR_4g116330 49.624 266 118 8 42 299 121 378 8.06e-73 229
MS.gene81242.t1 MTR_1g055175 60.733 191 72 3 111 298 8 198 2.37e-72 222
MS.gene81242.t1 MTR_4g023090 47.315 298 122 10 22 295 92 378 6.35e-72 227
MS.gene81242.t1 MTR_4g023120 46.984 315 137 11 4 297 61 366 7.03e-72 226
MS.gene81242.t1 MTR_4g023130 49.823 283 110 10 21 297 53 309 2.98e-70 221
MS.gene81242.t1 MTR_4g023380 44.590 305 111 9 23 295 93 371 5.79e-69 219
MS.gene81242.t1 MTR_1g055250 52.632 247 97 9 76 312 41 277 1.05e-67 214
MS.gene81242.t1 MTR_4g118400 48.084 287 108 12 20 295 38 294 2.60e-67 213
MS.gene81242.t1 MTR_8g032390 46.580 307 144 9 2 295 70 369 2.58e-66 212
MS.gene81242.t1 MTR_4g023190 46.711 304 128 12 9 297 71 355 8.59e-66 211
MS.gene81242.t1 MTR_4g023150 46.179 301 135 11 9 297 71 356 6.53e-65 208
MS.gene81242.t1 MTR_7g079370 43.849 317 152 11 9 304 48 359 4.69e-64 206
MS.gene81242.t1 MTR_4g023200 44.771 306 132 13 9 297 71 356 1.15e-63 205
MS.gene81242.t1 MTR_7g079400 44.406 286 143 8 21 295 51 331 1.51e-63 204
MS.gene81242.t1 MTR_4g116320 52.752 218 95 4 86 299 43 256 1.73e-60 194
MS.gene81242.t1 MTR_4g023440 42.997 307 123 10 1 301 72 332 9.81e-59 191
MS.gene81242.t1 MTR_8g433030 53.211 218 92 6 82 295 125 336 2.80e-58 191
MS.gene81242.t1 MTR_8g013010 53.774 212 80 8 1 203 107 309 6.08e-55 181
MS.gene81242.t1 MTR_8g040935 51.000 200 89 6 106 298 8 205 6.02e-53 172
MS.gene81242.t1 MTR_4g116330 43.233 266 96 9 42 299 121 339 8.89e-53 176
MS.gene81242.t1 MTR_4g121580 41.009 317 137 12 7 305 73 357 2.54e-52 176
MS.gene81242.t1 MTR_8g102520 42.231 251 129 8 56 296 73 317 2.97e-52 174
MS.gene81242.t1 MTR_6g463430 49.254 201 93 5 100 297 4 198 4.58e-46 155
MS.gene81242.t1 MTR_7g079420 39.405 269 111 9 44 295 905 1138 8.05e-46 166
MS.gene81242.t1 MTR_4g117530 43.636 220 79 7 80 297 73 249 5.17e-43 148
MS.gene81242.t1 MTR_2g048580 37.248 298 147 14 10 296 69 337 3.90e-42 149
MS.gene81242.t1 MTR_4g114700 39.051 274 102 10 45 306 9 229 1.28e-36 131
MS.gene81242.t1 MTR_4g118360 55.200 125 51 3 179 299 1 124 4.79e-36 126
MS.gene81242.t1 MTR_4g117430 47.097 155 64 4 55 203 10 152 1.80e-34 123
MS.gene81242.t1 MTR_4g116340 44.186 172 79 5 10 169 81 247 7.71e-33 122
MS.gene81242.t1 MTR_8g098830 41.358 162 90 4 112 270 35 194 4.56e-30 114
MS.gene81242.t1 MTR_5g023000 61.957 92 32 2 208 297 5 95 2.21e-28 105
MS.gene81242.t1 MTR_7g117720 48.936 141 58 6 4 133 47 184 3.94e-28 110
MS.gene81242.t1 MTR_7g117720 54.639 97 37 4 206 296 177 272 1.14e-20 90.1
MS.gene81242.t1 MTR_4g122860 36.139 202 94 6 117 311 157 330 1.03e-27 110
MS.gene81242.t1 MTR_7g079450 44.853 136 65 5 165 296 3 132 2.45e-26 101
MS.gene81242.t1 MTR_6g034720 37.500 168 83 5 68 226 53 207 1.64e-23 96.3
MS.gene81242.t1 MTR_4g133170 32.068 237 130 9 38 252 86 313 1.99e-23 99.4
MS.gene81242.t1 MTR_2g021190 29.236 301 183 12 7 293 88 372 2.05e-21 93.6
MS.gene81242.t1 MTR_1g055200 54.321 81 36 1 41 120 5 85 3.15e-20 84.3
MS.gene81242.t1 MTR_1g068835 30.841 214 119 8 38 231 87 291 1.77e-19 88.2
MS.gene81242.t1 MTR_2g021150 27.869 305 192 13 8 297 81 372 4.10e-19 87.0
MS.gene81242.t1 MTR_1g068860 30.189 212 125 7 38 231 87 293 8.31e-19 86.3
MS.gene81242.t1 MTR_5g057790 29.368 269 156 12 42 293 142 393 1.69e-18 85.5
MS.gene81242.t1 MTR_4g061340 31.963 219 108 10 37 231 114 315 1.88e-18 85.1
MS.gene81242.t1 MTR_2g078870 28.571 245 151 8 58 293 78 307 3.13e-18 84.0
MS.gene81242.t1 MTR_2g025960 30.364 247 132 11 42 256 5 243 3.39e-18 83.6
MS.gene81242.t1 MTR_1g068875 28.151 238 140 8 37 252 86 314 5.60e-18 84.0
MS.gene81242.t1 MTR_2g021155 31.250 240 136 9 42 264 130 357 5.76e-18 85.1
MS.gene81242.t1 MTR_3g006500 26.263 297 188 10 8 293 93 369 1.20e-17 82.8
MS.gene81242.t1 MTR_4g023430 51.807 83 34 3 224 301 141 222 2.56e-17 79.7
MS.gene81242.t1 MTR_2g025420 28.451 355 184 18 1 309 78 408 4.30e-17 81.6
MS.gene81242.t1 MTR_2g029990 27.372 274 173 11 33 293 142 402 1.08e-16 80.5
MS.gene81242.t1 MTR_4g070550 28.947 228 127 8 42 238 101 324 1.13e-16 80.1
MS.gene81242.t1 MTR_1g055145 57.333 75 30 2 145 218 129 202 1.96e-16 78.2
MS.gene81242.t1 MTR_3g030250 28.780 205 118 9 38 236 133 315 2.03e-16 79.3
MS.gene81242.t1 MTR_5g057710 32.353 204 111 10 42 232 142 331 2.66e-16 79.3
MS.gene81242.t1 MTR_0064s0160 26.429 280 182 8 39 298 148 423 3.26e-16 79.0
MS.gene81242.t1 MTR_2g021050 29.437 231 136 10 38 252 91 310 4.44e-16 78.6
MS.gene81242.t1 MTR_8g013005 65.385 52 18 0 246 297 4 55 4.76e-16 71.6
MS.gene81242.t1 MTR_3g007430 27.197 239 146 8 8 231 103 328 6.75e-16 77.8
MS.gene81242.t1 MTR_2g024100 28.740 254 139 9 38 256 92 338 7.01e-16 77.8
MS.gene81242.t1 MTR_2g026010 28.571 217 127 7 38 231 86 297 1.17e-15 77.4
MS.gene81242.t1 MTR_8g101580 27.843 255 151 14 56 293 156 394 1.50e-15 77.0
MS.gene81242.t1 MTR_5g069120 41.085 129 58 4 8 120 51 177 1.57e-15 73.6
MS.gene81242.t1 MTR_3g036290 30.435 253 132 14 40 275 117 342 1.66e-15 76.6
MS.gene81242.t1 MTR_6g053260 28.019 207 134 7 40 239 434 632 2.57e-15 77.0
MS.gene81242.t1 MTR_8g099460 26.349 315 179 13 18 296 71 368 3.42e-15 75.9
MS.gene81242.t1 MTR_8g046310 25.856 263 180 8 42 298 125 378 3.58e-15 75.9
MS.gene81242.t1 MTR_3g007330 30.000 210 123 9 42 239 137 334 8.06e-15 74.7
MS.gene81242.t1 MTR_3g023930 28.464 267 146 13 43 293 130 367 8.39e-15 74.7
MS.gene81242.t1 MTR_3g010920 25.839 298 182 9 7 293 112 381 1.02e-14 74.3
MS.gene81242.t1 MTR_3g009310 33.333 186 93 10 58 231 160 326 1.12e-14 74.3
MS.gene81242.t1 MTR_1g094210 27.138 269 161 11 39 293 151 398 1.18e-14 74.3
MS.gene81242.t1 MTR_4g117550 26.199 271 150 10 46 294 127 369 1.42e-14 73.9
MS.gene81242.t1 MTR_3g024130 26.403 303 171 16 8 275 16 301 2.19e-14 72.8
MS.gene81242.t1 MTR_1g094210 27.347 245 144 9 40 270 152 376 2.85e-14 73.2
MS.gene81242.t1 MTR_1g097560 26.446 242 150 11 39 269 128 352 3.25e-14 72.8
MS.gene81242.t1 MTR_4g055060 30.222 225 118 11 34 236 119 326 3.78e-14 72.8
MS.gene81242.t1 MTR_2g055970 28.986 207 127 9 36 232 301 497 4.16e-14 73.2
MS.gene81242.t1 MTR_3g007410 30.631 222 117 10 42 241 145 351 6.27e-14 72.0
MS.gene81242.t1 MTR_6g053240 26.415 212 136 7 40 239 125 328 6.60e-14 72.0
MS.gene81242.t1 MTR_2g426060 27.626 257 142 12 32 252 88 336 8.71e-14 71.6
MS.gene81242.t1 MTR_6g027890 28.413 271 152 11 52 293 67 324 1.16e-13 70.9
MS.gene81242.t1 MTR_3g007360 30.396 227 111 12 42 241 145 351 1.25e-13 71.2
MS.gene81242.t1 MTR_2g031640 26.263 297 182 10 38 308 88 373 1.28e-13 71.2
MS.gene81242.t1 MTR_4g023100 51.429 70 30 2 232 301 54 119 1.38e-13 66.6
MS.gene81242.t1 MTR_2g031720 26.756 299 180 12 42 312 96 383 2.09e-13 70.5
MS.gene81242.t1 MTR_2g028130 26.160 237 140 9 42 249 106 336 2.10e-13 70.5
MS.gene81242.t1 MTR_1g054725 26.715 277 160 11 42 293 117 375 2.10e-13 70.5
MS.gene81242.t1 MTR_5g059430 25.874 286 166 12 45 292 143 420 2.53e-13 70.5
MS.gene81242.t1 MTR_5g097680 27.465 284 148 15 54 294 155 423 2.61e-13 70.5
MS.gene81242.t1 MTR_3g013740 29.858 211 112 9 42 232 149 343 3.50e-13 70.1
MS.gene81242.t1 MTR_1g097490 26.616 263 172 9 39 293 141 390 4.43e-13 69.7
MS.gene81242.t1 MTR_3g010970 26.638 229 136 10 10 236 127 325 4.44e-13 69.7
MS.gene81242.t1 MTR_0115s0020 26.014 296 166 15 39 294 113 395 4.55e-13 69.7
MS.gene81242.t1 MTR_3g010870 26.007 273 164 10 40 293 131 384 4.71e-13 69.3
MS.gene81242.t1 MTR_8g063440 26.224 286 167 14 37 294 110 379 4.92e-13 69.3
MS.gene81242.t1 MTR_2g031680 27.526 287 165 12 38 296 87 358 5.60e-13 69.3
MS.gene81242.t1 MTR_3g024150 29.293 198 113 9 43 232 139 317 1.00e-12 68.6
MS.gene81242.t1 MTR_8g046200 25.083 303 172 11 42 298 129 422 1.15e-12 68.6
MS.gene81242.t1 MTR_5g057690 28.643 199 123 7 38 232 138 321 1.58e-12 67.8
MS.gene81242.t1 MTR_6g048120 27.925 265 143 12 58 293 162 407 1.88e-12 67.8
MS.gene81242.t1 MTR_2g104340 25.246 305 181 11 27 294 98 392 2.11e-12 67.4
MS.gene81242.t1 MTR_4g122840 36.719 128 44 4 171 295 165 258 2.12e-12 66.6
MS.gene81242.t1 MTR_2g078900 30.556 180 110 6 58 236 153 318 2.31e-12 67.4
MS.gene81242.t1 MTR_2g021210 27.801 241 148 10 36 266 173 397 2.91e-12 67.4
MS.gene81242.t1 MTR_0054s0090 23.469 294 172 13 42 294 121 402 3.29e-12 67.0
MS.gene81242.t1 MTR_3g006640 27.907 215 118 8 42 232 20 221 3.32e-12 66.2
MS.gene81242.t1 MTR_5g057020 28.986 207 123 9 38 240 138 324 3.42e-12 66.6
MS.gene81242.t1 MTR_4g031770 29.126 206 124 9 42 236 100 294 3.53e-12 66.6
MS.gene81242.t1 MTR_4g024890 23.368 291 175 11 39 293 106 384 3.93e-12 66.6
MS.gene81242.t1 MTR_2g021252 25.954 262 165 9 42 294 144 385 4.70e-12 66.6
MS.gene81242.t1 MTR_3g007300 29.851 201 107 9 38 231 129 302 5.37e-12 66.2
MS.gene81242.t1 MTR_5g009060 32.075 212 101 12 42 227 144 338 6.69e-12 66.2
MS.gene81242.t1 MTR_2g066200 26.977 215 126 8 58 251 137 341 9.25e-12 65.5
MS.gene81242.t1 MTR_6g021880 28.839 267 167 11 43 293 122 381 1.13e-11 65.5
MS.gene81242.t1 MTR_3g006670 28.365 208 116 8 37 227 141 332 1.56e-11 65.1
MS.gene81242.t1 MTR_5g097330 25.862 290 177 12 31 294 122 399 1.58e-11 65.1
MS.gene81242.t1 MTR_8g464770 26.259 278 173 12 38 295 112 377 1.76e-11 64.7
MS.gene81242.t1 MTR_3g010520 27.670 206 111 11 40 236 119 295 1.90e-11 64.7
MS.gene81242.t1 MTR_3g009240 30.256 195 117 8 42 227 128 312 2.00e-11 64.7
MS.gene81242.t1 MTR_3g030310 28.042 189 116 6 53 237 144 316 2.01e-11 64.7
MS.gene81242.t1 MTR_5g022420 26.531 196 127 7 40 232 120 301 2.10e-11 64.3
MS.gene81242.t1 MTR_3g014540 30.189 212 117 12 42 236 123 320 2.27e-11 64.3
MS.gene81242.t1 MTR_0054s0100 23.469 294 170 13 42 292 121 402 2.27e-11 64.3
MS.gene81242.t1 MTR_6g086825 26.000 300 171 14 33 292 166 454 2.34e-11 64.7
MS.gene81242.t1 MTR_2g425980 28.507 221 121 8 42 231 98 312 2.72e-11 64.3
MS.gene81242.t1 MTR_6g090180 24.324 296 180 12 36 294 146 434 5.43e-11 63.5
MS.gene81242.t1 MTR_7g069550 22.635 296 177 12 7 261 80 364 5.65e-11 63.2
MS.gene81242.t1 MTR_3g013770 29.524 210 122 9 40 236 132 328 5.69e-11 63.2
MS.gene81242.t1 MTR_1g097330 28.079 203 125 9 40 232 107 298 5.92e-11 63.2
MS.gene81242.t1 MTR_5g046260 25.381 197 111 8 33 227 87 249 8.18e-11 62.4
MS.gene81242.t1 MTR_7g407070 27.948 229 116 11 42 239 135 345 9.45e-11 62.8
MS.gene81242.t1 MTR_1g097370 26.267 217 139 10 48 252 104 311 9.48e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81242.t1 AT3G06240 29.670 273 164 12 38 296 163 421 8.70e-19 86.7
MS.gene81242.t1 AT3G23880 26.199 271 170 12 39 297 111 363 2.52e-12 67.4

Find 53 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTTCGATATTGAAATTAA+AGG 0.208263 5.2:-81496709 MS.gene81242:CDS
GGTCACTGTCAGCTTGTCAT+TGG 0.288922 5.2:+81495928 None:intergenic
TTAGAGGATTTCTCAGTCTA+TGG 0.289404 5.2:-81496222 MS.gene81242:CDS
GTCACTGTCAGCTTGTCATT+GGG 0.292686 5.2:+81495929 None:intergenic
ATTTAACTACCTTATTCTTC+TGG 0.298545 5.2:+81495884 None:intergenic
TATTGTTGCCTTTCATTTAA+CGG 0.314412 5.2:-81496317 MS.gene81242:CDS
GAAGAGACATTAGTATAAAA+AGG 0.325623 5.2:+81496418 None:intergenic
GAATTATAATTAATATCAAC+TGG 0.339568 5.2:+81496268 None:intergenic
TAGGGAATGTCATCATAAAT+AGG 0.340881 5.2:+81496118 None:intergenic
GGGACGATGAAGTTGATATA+TGG 0.344274 5.2:-81496192 MS.gene81242:CDS
CGCAACAGATGATTATTTAG+TGG 0.344517 5.2:-81496551 MS.gene81242:CDS
AATACAAAGTGCATTCATCT+TGG 0.393478 5.2:-81496159 MS.gene81242:CDS
TCTTCAATGGGGCTATTCAC+TGG 0.420006 5.2:-81496372 MS.gene81242:CDS
ACGCACAAACCAGAAGAATA+AGG 0.424832 5.2:-81495893 None:intergenic
TATTTGTTTGTTACGTCCAA+TGG 0.431181 5.2:+81496627 None:intergenic
CAATGGGGCTATTCACTGGT+TGG 0.434685 5.2:-81496368 MS.gene81242:CDS
CAAGTAGAGTCGTTCTTCAA+TGG 0.436469 5.2:-81496385 MS.gene81242:CDS
AAGTAGAGTCGTTCTTCAAT+GGG 0.446028 5.2:-81496384 MS.gene81242:CDS
AATTATAATTCCAAAGGTTG+TGG 0.459804 5.2:-81496256 MS.gene81242:CDS
TTATACTAATGTCTCTTCCT+CGG 0.466914 5.2:-81496413 MS.gene81242:CDS
ATTGGAACAGATGGTGGTAC+TGG 0.468517 5.2:-81496058 MS.gene81242:CDS
TGAGTTTGAAATTTGATCAC+GGG 0.471501 5.2:+81496519 None:intergenic
TTTCCTTTCCGTTAAATGAA+AGG 0.478785 5.2:+81496309 None:intergenic
TCCACGTGCATCTTCACAAT+AGG 0.482064 5.2:+81495991 None:intergenic
TTTGTGCGTCTTCTTAAGCT+TGG 0.484874 5.2:+81495907 None:intergenic
TAGGATAAAGGGCAAAAGTA+GGG 0.505069 5.2:+81496100 None:intergenic
TACCTATGGAATCCATCCAT+TGG 0.507663 5.2:-81496643 MS.gene81242:CDS
TTGCCTTTCATTTAACGGAA+AGG 0.511796 5.2:-81496312 MS.gene81242:CDS
AAATTAAAGGTTCGTGTAGA+GGG 0.515124 5.2:-81496696 MS.gene81242:CDS
CTGAGTTTGAAATTTGATCA+CGG 0.522327 5.2:+81496518 None:intergenic
ATTGAGAGCTAATGTGTGGC+AGG 0.523321 5.2:-81496464 MS.gene81242:CDS
GAAATTAAAGGTTCGTGTAG+AGG 0.532265 5.2:-81496697 MS.gene81242:CDS
GGACGATGAAGTTGATATAT+GGG 0.532674 5.2:-81496191 MS.gene81242:CDS
ATAGGATAAAGGGCAAAAGT+AGG 0.534631 5.2:+81496099 None:intergenic
GATATTAATTATAATTCCAA+AGG 0.542638 5.2:-81496262 MS.gene81242:CDS
TGTTTGTTACGTCCAATGGA+TGG 0.559201 5.2:+81496631 None:intergenic
TCACTGTCAGCTTGTCATTG+GGG 0.567864 5.2:+81495930 None:intergenic
GGTGATATTATTGGAACAGA+TGG 0.576103 5.2:-81496067 MS.gene81242:CDS
ACTCTGTATACATAGCCAAT+CGG 0.578596 5.2:+81495966 None:intergenic
ATGATGTGCTATCTCACTTG+TGG 0.589833 5.2:-81496495 MS.gene81242:CDS
TCAATATCGAAATAAGATTG+TGG 0.600503 5.2:+81496718 None:intergenic
AGATGCACGTGGATTCCGAT+TGG 0.602170 5.2:-81495981 MS.gene81242:CDS
ATATAATCATTAGGAGACAA+AGG 0.607813 5.2:+81496604 None:intergenic
AACAGATGGTGGTACTGGAT+TGG 0.625571 5.2:-81496053 MS.gene81242:CDS
CGACTCTACTTGTCGAACCG+AGG 0.630880 5.2:+81496396 None:intergenic
AATTATAATTAATATCAACT+GGG 0.634811 5.2:+81496269 None:intergenic
TTGGTGAAATATAACGACAA+AGG 0.634925 5.2:-81496034 MS.gene81242:CDS
TCTCATTGAGAGCTAATGTG+TGG 0.640904 5.2:-81496468 MS.gene81242:CDS
AATGAAAGGCAACAATAACA+TGG 0.652147 5.2:+81496323 None:intergenic
GATATTATTGGAACAGATGG+TGG 0.666571 5.2:-81496064 MS.gene81242:CDS
GTCCAATGGATGGATTCCAT+AGG 0.673456 5.2:+81496641 None:intergenic
AGTAGAGTCGTTCTTCAATG+GGG 0.705724 5.2:-81496383 MS.gene81242:CDS
TCCTATTGTGAAGATGCACG+TGG 0.711389 5.2:-81495992 MS.gene81242:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATAATTAATATCAACT+GGG + chr5.2:81496440-81496459 None:intergenic 10.0%
!!! ATTTTTAATTTATATGGTTT+TGG - chr5.2:81496123-81496142 MS.gene81242:CDS 10.0%
!!! TTTTTAATTTATATGGTTTT+GGG - chr5.2:81496124-81496143 MS.gene81242:CDS 10.0%
!! GAATTATAATTAATATCAAC+TGG + chr5.2:81496441-81496460 None:intergenic 15.0%
!! GATATTAATTATAATTCCAA+AGG - chr5.2:81496444-81496463 MS.gene81242:CDS 15.0%
!! TATTTCGATATTGAAATTAA+AGG - chr5.2:81495997-81496016 MS.gene81242:CDS 15.0%
! AATTATAATTCCAAAGGTTG+TGG - chr5.2:81496450-81496469 MS.gene81242:CDS 25.0%
! ACAAAAAGTGGTGATATTAT+TGG - chr5.2:81496630-81496649 MS.gene81242:CDS 25.0%
! ATATAATCATTAGGAGACAA+AGG + chr5.2:81496105-81496124 None:intergenic 25.0%
! ATTAGTATAAAAAGGCAAAC+GGG + chr5.2:81496283-81496302 None:intergenic 25.0%
! CATTAGTATAAAAAGGCAAA+CGG + chr5.2:81496284-81496303 None:intergenic 25.0%
! GAAGAGACATTAGTATAAAA+AGG + chr5.2:81496291-81496310 None:intergenic 25.0%
! TATTGTTGCCTTTCATTTAA+CGG - chr5.2:81496389-81496408 MS.gene81242:CDS 25.0%
! TCAATATCGAAATAAGATTG+TGG + chr5.2:81495991-81496010 None:intergenic 25.0%
!!! AATATCACCACTTTTTGTAT+AGG + chr5.2:81496628-81496647 None:intergenic 25.0%
!!! CACTTTTTGTATAGGATAAA+GGG + chr5.2:81496620-81496639 None:intergenic 25.0%
!!! TCTATAGATTGAGTTTTAAG+AGG + chr5.2:81495916-81495935 None:intergenic 25.0%
AAATTAAAGGTTCGTGTAGA+GGG - chr5.2:81496010-81496029 MS.gene81242:CDS 30.0%
AATACAAAGTGCATTCATCT+TGG - chr5.2:81496547-81496566 MS.gene81242:CDS 30.0%
AATGAAAGGCAACAATAACA+TGG + chr5.2:81496386-81496405 None:intergenic 30.0%
CCTTTATCCTATACAAAAAG+TGG - chr5.2:81496618-81496637 MS.gene81242:CDS 30.0%
TAGGGAATGTCATCATAAAT+AGG + chr5.2:81496591-81496610 None:intergenic 30.0%
TTATACTAATGTCTCTTCCT+CGG - chr5.2:81496293-81496312 MS.gene81242:CDS 30.0%
TTGGTGAAATATAACGACAA+AGG - chr5.2:81496672-81496691 MS.gene81242:CDS 30.0%
TTTCCTTTCCGTTAAATGAA+AGG + chr5.2:81496400-81496419 None:intergenic 30.0%
! CTGAGTTTGAAATTTGATCA+CGG + chr5.2:81496191-81496210 None:intergenic 30.0%
! TATTTGTTTGTTACGTCCAA+TGG + chr5.2:81496082-81496101 None:intergenic 30.0%
! TGAGTTTGAAATTTGATCAC+GGG + chr5.2:81496190-81496209 None:intergenic 30.0%
!!! ATAGATTGAGTTTTAAGAGG+TGG + chr5.2:81495913-81495932 None:intergenic 30.0%
!!! CCACTTTTTGTATAGGATAA+AGG + chr5.2:81496621-81496640 None:intergenic 30.0%
AAGTAGAGTCGTTCTTCAAT+GGG - chr5.2:81496322-81496341 MS.gene81242:CDS 35.0%
ACTCTGTATACATAGCCAAT+CGG + chr5.2:81496743-81496762 None:intergenic 35.0%
ATAGGATAAAGGGCAAAAGT+AGG + chr5.2:81496610-81496629 None:intergenic 35.0%
CGCAACAGATGATTATTTAG+TGG - chr5.2:81496155-81496174 MS.gene81242:CDS 35.0%
GAAATTAAAGGTTCGTGTAG+AGG - chr5.2:81496009-81496028 MS.gene81242:CDS 35.0%
GATATTATTGGAACAGATGG+TGG - chr5.2:81496642-81496661 MS.gene81242:CDS 35.0%
GGTGATATTATTGGAACAGA+TGG - chr5.2:81496639-81496658 MS.gene81242:CDS 35.0%
TAGGATAAAGGGCAAAAGTA+GGG + chr5.2:81496609-81496628 None:intergenic 35.0%
TTAGAGGATTTCTCAGTCTA+TGG - chr5.2:81496484-81496503 MS.gene81242:CDS 35.0%
TTGCCTTTCATTTAACGGAA+AGG - chr5.2:81496394-81496413 MS.gene81242:CDS 35.0%
! ATTCCAAAGGTTGTGGTTTT+TGG - chr5.2:81496457-81496476 MS.gene81242:CDS 35.0%
! GGGTTTATACTTTTGCACTT+TGG - chr5.2:81496030-81496049 MS.gene81242:CDS 35.0%
! TCTCAGTCTATGGATTTTGA+GGG - chr5.2:81496494-81496513 MS.gene81242:CDS 35.0%
! TTCCAAAGGTTGTGGTTTTT+GGG - chr5.2:81496458-81496477 MS.gene81242:CDS 35.0%
! TTCTCAGTCTATGGATTTTG+AGG - chr5.2:81496493-81496512 MS.gene81242:CDS 35.0%
!! GGACGATGAAGTTGATATAT+GGG - chr5.2:81496515-81496534 MS.gene81242:CDS 35.0%
!!! TGTGGTTTTTGGGTATTTAG+AGG - chr5.2:81496468-81496487 MS.gene81242:CDS 35.0%
AGTAGAGTCGTTCTTCAATG+GGG - chr5.2:81496323-81496342 MS.gene81242:CDS 40.0%
ATGATGTGCTATCTCACTTG+TGG - chr5.2:81496211-81496230 MS.gene81242:CDS 40.0%
CAAGTAGAGTCGTTCTTCAA+TGG - chr5.2:81496321-81496340 MS.gene81242:CDS 40.0%
TACCCAAAAACCACAACCTT+TGG + chr5.2:81496463-81496482 None:intergenic 40.0%
TACCTATGGAATCCATCCAT+TGG - chr5.2:81496063-81496082 MS.gene81242:CDS 40.0%
TCTCATTGAGAGCTAATGTG+TGG - chr5.2:81496238-81496257 MS.gene81242:CDS 40.0%
TTTGTGCGTCTTCTTAAGCT+TGG + chr5.2:81496802-81496821 None:intergenic 40.0%
! TGTTTGTTACGTCCAATGGA+TGG + chr5.2:81496078-81496097 None:intergenic 40.0%
! TTGGTGCATGCTTTTACCTA+TGG - chr5.2:81496049-81496068 MS.gene81242:CDS 40.0%
!! GGGACGATGAAGTTGATATA+TGG - chr5.2:81496514-81496533 MS.gene81242:CDS 40.0%
ATTGAGAGCTAATGTGTGGC+AGG - chr5.2:81496242-81496261 MS.gene81242:CDS 45.0%
GTCACTGTCAGCTTGTCATT+GGG + chr5.2:81496780-81496799 None:intergenic 45.0%
TCACTGTCAGCTTGTCATTG+GGG + chr5.2:81496779-81496798 None:intergenic 45.0%
TCCACGTGCATCTTCACAAT+AGG + chr5.2:81496718-81496737 None:intergenic 45.0%
TCCTATTGTGAAGATGCACG+TGG - chr5.2:81496714-81496733 MS.gene81242:CDS 45.0%
TCTTCAATGGGGCTATTCAC+TGG - chr5.2:81496334-81496353 MS.gene81242:CDS 45.0%
! AACAGATGGTGGTACTGGAT+TGG - chr5.2:81496653-81496672 MS.gene81242:CDS 45.0%
! ATTGGAACAGATGGTGGTAC+TGG - chr5.2:81496648-81496667 MS.gene81242:CDS 45.0%
! GTCCAATGGATGGATTCCAT+AGG + chr5.2:81496068-81496087 None:intergenic 45.0%
!! AAATTAAAAATATAATCATT+AGG + chr5.2:81496114-81496133 None:intergenic 5.0%
!!! GATTATATTTTTAATTTATA+TGG - chr5.2:81496117-81496136 MS.gene81242:CDS 5.0%
AGATGCACGTGGATTCCGAT+TGG - chr5.2:81496725-81496744 MS.gene81242:CDS 50.0%
CAATGGGGCTATTCACTGGT+TGG - chr5.2:81496338-81496357 MS.gene81242:CDS 50.0%
GGTCACTGTCAGCTTGTCAT+TGG + chr5.2:81496781-81496800 None:intergenic 50.0%
CGACTCTACTTGTCGAACCG+AGG + chr5.2:81496313-81496332 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 81495895 81496833 81495895 ID=MS.gene81242
chr5.2 mRNA 81495895 81496833 81495895 ID=MS.gene81242.t1;Parent=MS.gene81242
chr5.2 exon 81495895 81496833 81495895 ID=MS.gene81242.t1.exon1;Parent=MS.gene81242.t1
chr5.2 CDS 81495895 81496833 81495895 ID=cds.MS.gene81242.t1;Parent=MS.gene81242.t1
Gene Sequence

>MS.gene81242

ATGCTCATATTAACTCCACCTCTTAAAACTCAATCTATAGATATAGAGCTATCACTTTACGCTTCTGCTTCAATGAACAATAATTTTTTGTGTCCACAATCTTATTTCGATATTGAAATTAAAGGTTCGTGTAGAGGGTTTATACTTTTGCACTTTGGTGCATGCTTTTACCTATGGAATCCATCCATTGGACGTAACAAACAAATACCTTTGTCTCCTAATGATTATATTTTTAATTTATATGGTTTTGGGTATGATCACGCAACAGATGATTATTTAGTGGTTTCAATTTCCCGTGATCAAATTTCAAACTCAGATGATGTGCTATCTCACTTGTGGCTTTTCTCATTGAGAGCTAATGTGTGGCAGGAAATTGCATGCACAACCCGTTTGCCTTTTTATACTAATGTCTCTTCCTCGGTTCGACAAGTAGAGTCGTTCTTCAATGGGGCTATTCACTGGTTGGCTTTTCGTCATGATATATCTGACCATGTTATTGTTGCCTTTCATTTAACGGAAAGGAAACTTCTAGAGATTCTTCTCCCAGTTGATATTAATTATAATTCCAAAGGTTGTGGTTTTTGGGTATTTAGAGGATTTCTCAGTCTATGGATTTTGAGGGACGATGAAGTTGATATATGGGTGATGAAAGAATACAAAGTGCATTCATCTTGGATTAAAACTCTTGTTCTTCCTATTTATGATGACATTCCCTACTTTTGCCCTTTATCCTATACAAAAAGTGGTGATATTATTGGAACAGATGGTGGTACTGGATTGGTGAAATATAACGACAAAGGAGAGATTCTAGAGCATAACTCCTATTGTGAAGATGCACGTGGATTCCGATTGGCTATGTATACAGAGTCATTGCTTTCGCTCCCCAATGACAAGCTGACAGTGACCAAGCTTAAGAAGACGCACAAACCAGAAGAATAA
Protein sequence

>MS.gene81242.t1

MLILTPPLKTQSIDIELSLYASASMNNNFLCPQSYFDIEIKGSCRGFILLHFGACFYLWNPSIGRNKQIPLSPNDYIFNLYGFGYDHATDDYLVVSISRDQISNSDDVLSHLWLFSLRANVWQEIACTTRLPFYTNVSSSVRQVESFFNGAIHWLAFRHDISDHVIVAFHLTERKLLEILLPVDINYNSKGCGFWVFRGFLSLWILRDDEVDIWVMKEYKVHSSWIKTLVLPIYDDIPYFCPLSYTKSGDIIGTDGGTGLVKYNDKGEILEHNSYCEDARGFRLAMYTESLLSLPNDKLTVTKLKKTHKPEE