AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064109


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064109.t1 MTR_4g023080 72.678 183 42 4 2 183 178 353 2.29e-86 258
MS.gene064109.t1 MTR_4g023130 70.492 183 46 4 2 183 137 312 2.02e-83 249
MS.gene064109.t1 MTR_4g023070 62.564 195 56 2 2 184 181 370 3.59e-80 243
MS.gene064109.t1 MTR_4g021785 68.108 185 50 4 1 183 177 354 7.01e-79 239
MS.gene064109.t1 MTR_4g023090 61.026 195 58 2 2 183 194 383 1.11e-77 237
MS.gene064109.t1 MTR_4g023120 60.309 194 60 2 2 183 181 369 1.74e-75 231
MS.gene064109.t1 MTR_4g023380 60.488 205 53 3 2 183 179 378 1.85e-75 231
MS.gene064109.t1 MTR_3g103020 57.527 186 71 4 2 183 196 377 2.80e-63 200
MS.gene064109.t1 MTR_4g116320 59.563 183 67 5 1 181 78 255 1.21e-62 194
MS.gene064109.t1 MTR_4g109280 52.717 184 80 5 2 183 180 358 1.73e-59 189
MS.gene064109.t1 MTR_6g060650 54.098 183 77 4 2 183 196 372 1.50e-58 187
MS.gene064109.t1 MTR_4g117720 56.757 185 72 6 2 181 194 375 2.50e-58 187
MS.gene064109.t1 MTR_4g023200 55.435 184 74 4 2 182 180 358 4.34e-58 186
MS.gene064109.t1 MTR_4g116330 55.249 181 78 3 2 181 199 377 1.20e-57 185
MS.gene064109.t1 MTR_4g109270 54.595 185 77 4 2 183 183 363 1.26e-57 185
MS.gene064109.t1 MTR_4g023190 55.978 184 72 5 2 182 180 357 4.17e-56 181
MS.gene064109.t1 MTR_8g040935 51.872 187 84 4 1 183 23 207 1.70e-55 174
MS.gene064109.t1 MTR_4g094365 55.376 186 73 6 2 183 184 363 2.55e-55 179
MS.gene064109.t1 MTR_4g023150 54.348 184 76 4 2 182 180 358 2.06e-54 176
MS.gene064109.t1 MTR_6g463430 54.098 183 74 3 2 182 26 200 9.09e-54 170
MS.gene064109.t1 MTR_8g433030 54.098 183 71 7 2 179 163 337 4.19e-52 170
MS.gene064109.t1 MTR_8g032390 54.098 183 71 7 2 179 196 370 9.94e-52 170
MS.gene064109.t1 MTR_3g034180 53.684 190 73 6 2 183 306 488 2.15e-51 172
MS.gene064109.t1 MTR_4g117850 51.075 186 82 5 1 181 195 376 5.68e-51 168
MS.gene064109.t1 MTR_4g023440 51.099 182 46 2 2 183 193 331 1.19e-49 163
MS.gene064109.t1 MTR_3g034130 52.778 180 72 5 2 171 192 368 2.03e-49 164
MS.gene064109.t1 MTR_5g070130 47.568 185 85 3 2 177 188 369 7.71e-49 162
MS.gene064109.t1 MTR_4g117780 51.613 186 83 3 2 183 184 366 1.16e-48 162
MS.gene064109.t1 MTR_3g034160 53.191 188 73 5 2 181 192 372 4.64e-48 160
MS.gene064109.t1 MTR_4g122850 53.439 189 74 10 2 183 182 363 2.86e-46 156
MS.gene064109.t1 MTR_4g117770 51.075 186 84 3 2 183 184 366 5.64e-46 155
MS.gene064109.t1 MTR_4g117810 51.075 186 84 3 2 183 184 366 5.64e-46 155
MS.gene064109.t1 MTR_1g055195 54.839 155 62 4 24 177 324 471 1.00e-45 157
MS.gene064109.t1 MTR_4g118380 52.201 159 63 4 2 150 198 353 4.45e-44 149
MS.gene064109.t1 MTR_5g097210 49.206 189 75 8 1 180 183 359 4.80e-44 150
MS.gene064109.t1 MTR_8g433040 48.925 186 74 5 2 183 195 363 6.01e-44 149
MS.gene064109.t1 MTR_8g032380 48.925 186 74 5 2 183 195 363 6.01e-44 149
MS.gene064109.t1 MTR_5g070088 50.633 158 75 1 25 182 229 383 7.06e-44 150
MS.gene064109.t1 MTR_1g055175 46.277 188 87 8 2 182 19 199 7.55e-42 139
MS.gene064109.t1 MTR_5g070080 48.619 181 84 6 2 179 206 380 2.65e-41 143
MS.gene064109.t1 MTR_4g118400 46.927 179 74 5 1 177 134 293 3.51e-41 140
MS.gene064109.t1 MTR_1g055165 48.235 170 80 6 15 183 155 317 4.77e-40 138
MS.gene064109.t1 MTR_1g055250 42.623 183 93 7 1 177 87 263 1.15e-38 135
MS.gene064109.t1 MTR_4g117870 46.369 179 57 5 2 180 124 263 2.80e-37 130
MS.gene064109.t1 MTR_1g055135 47.239 163 78 5 24 183 217 374 3.42e-37 132
MS.gene064109.t1 MTR_4g117530 42.077 183 68 4 2 183 107 252 3.47e-37 129
MS.gene064109.t1 MTR_4g023430 71.250 80 23 0 104 183 142 221 4.00e-35 123
MS.gene064109.t1 MTR_4g116330 42.541 181 62 3 2 181 199 338 4.84e-35 125
MS.gene064109.t1 MTR_5g023000 61.386 101 36 1 83 183 1 98 2.17e-33 114
MS.gene064109.t1 MTR_7g079400 42.784 194 91 9 2 183 151 336 1.52e-32 119
MS.gene064109.t1 MTR_4g117750 50.370 135 57 2 55 182 350 481 3.77e-32 120
MS.gene064109.t1 MTR_4g117750 44.271 192 87 7 1 180 172 355 3.94e-30 115
MS.gene064109.t1 MTR_4g118370 40.323 186 81 6 1 181 97 257 1.24e-31 115
MS.gene064109.t1 MTR_7g079370 40.437 183 94 6 2 177 175 349 2.15e-31 116
MS.gene064109.t1 MTR_4g121580 45.614 171 57 7 1 165 195 335 1.94e-30 114
MS.gene064109.t1 MTR_4g118360 55.856 111 44 3 71 181 18 123 2.44e-30 107
MS.gene064109.t1 MTR_8g102520 39.181 171 90 6 20 183 158 321 2.07e-27 105
MS.gene064109.t1 MTR_4g122860 40.426 188 76 9 2 183 162 319 1.28e-24 98.2
MS.gene064109.t1 MTR_7g079450 42.466 146 73 5 39 183 1 136 1.69e-23 90.9
MS.gene064109.t1 MTR_7g079420 41.818 165 76 9 21 177 985 1137 2.02e-23 97.1
MS.gene064109.t1 MTR_8g098830 39.610 154 78 6 24 175 72 212 2.98e-23 92.4
MS.gene064109.t1 MTR_7g117720 61.628 86 30 3 94 177 186 270 6.65e-22 90.1
MS.gene064109.t1 MTR_4g023100 72.881 59 11 1 125 183 65 118 2.23e-21 84.7
MS.gene064109.t1 MTR_4g114700 34.807 181 66 3 1 178 90 221 5.21e-21 87.0
MS.gene064109.t1 MTR_2g048580 37.297 185 90 11 2 177 168 335 3.54e-20 86.3
MS.gene064109.t1 MTR_4g117430 53.659 82 37 1 2 83 75 155 5.14e-20 82.4
MS.gene064109.t1 MTR_8g013010 53.659 82 28 4 2 80 235 309 5.65e-16 74.3
MS.gene064109.t1 MTR_4g122840 38.849 139 45 5 46 183 164 263 7.62e-12 62.4
MS.gene064109.t1 MTR_8g013005 56.667 60 21 2 125 183 3 58 1.48e-11 57.4
MS.gene064109.t1 MTR_1g055145 47.727 88 39 5 11 97 121 202 1.57e-11 61.6
MS.gene064109.t1 MTR_4g109290 60.000 55 19 2 129 183 136 187 5.58e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064109.t1 AT3G06240 31.056 161 97 6 21 176 268 419 9.84e-12 62.8

Find 41 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGTTGCCTTTGATTTAA+TGG 0.113056 4.3:-75365978 MS.gene064109:CDS
TTTGATGGTTTGTGGGTATT+TGG 0.242237 4.3:-75365899 MS.gene064109:CDS
TTGGAGAATTTCTAAGTCTA+TGG 0.259076 4.3:-75365880 MS.gene064109:CDS
AAGAAAGCATTTCTATTTGA+TGG 0.290942 4.3:-75365914 MS.gene064109:CDS
ATGATAAAGGACAACTATTA+TGG 0.306500 4.3:-75365670 MS.gene064109:CDS
CCTTTAGTGGGGCTGTTCAT+TGG 0.312684 4.3:-75366033 MS.gene064109:CDS
TTAATGATACAACTGTAATA+TGG 0.316712 4.3:-75365844 MS.gene064109:CDS
TGTAAGAGTCCATATGAAAT+TGG 0.319752 4.3:-75365635 MS.gene064109:CDS
GCTTTCTTGTCAAAATCATT+TGG 0.335595 4.3:+75365929 None:intergenic
GATATTATTGGAAGAAATCT+TGG 0.373623 4.3:-75365713 MS.gene064109:CDS
CATTTCTATTTGATGGTTTG+TGG 0.390994 4.3:-75365907 MS.gene064109:CDS
GAGTCTCTGCTTTCACTCCT+TGG 0.393410 4.3:-75365587 MS.gene064109:CDS
TTGTCAAAATCATTTGGAAC+TGG 0.400066 4.3:+75365935 None:intergenic
GAGAAGGGGTTCCCTTTAGT+GGG 0.408337 4.3:-75366045 MS.gene064109:CDS
GGAGAAGGGGTTCCCTTTAG+TGG 0.409358 4.3:-75366046 MS.gene064109:CDS
ATAGGTTGAAAGAAGTGATT+AGG 0.414422 4.3:+75365755 None:intergenic
AAGAAATCTTGGTACTAGAT+TGG 0.428204 4.3:-75365702 MS.gene064109:CDS
ATTTCTATTTGATGGTTTGT+GGG 0.428632 4.3:-75365906 MS.gene064109:CDS
GTATACATGCCAATTTCATA+TGG 0.442449 4.3:+75365626 None:intergenic
CAATGAACAGCCCCACTAAA+GGG 0.448820 4.3:+75366034 None:intergenic
TAGTGGGGCTGTTCATTGGT+TGG 0.462169 4.3:-75366029 MS.gene064109:CDS
TAATATGTGGAAAGAAATAG+AGG 0.468516 4.3:-75366104 None:intergenic
TTTCCTTTCCATTAAATCAA+AGG 0.474723 4.3:+75365970 None:intergenic
CCAATGAACAGCCCCACTAA+AGG 0.489470 4.3:+75366033 None:intergenic
TGGAGAATTTCTAAGTCTAT+GGG 0.490268 4.3:-75365879 MS.gene064109:CDS
TTGGTGAAGTATAATGATAA+AGG 0.492510 4.3:-75365683 MS.gene064109:CDS
ATTTCTAAGTCTATGGGCTA+TGG 0.507502 4.3:-75365873 MS.gene064109:CDS
TAATGATACAACTGTAATAT+GGG 0.523695 4.3:-75365843 MS.gene064109:CDS
TATATTGACGTATACAAAGA+TGG 0.527306 4.3:-75366067 MS.gene064109:CDS
TGACATCTAAACGTAAATCA+TGG 0.533252 4.3:+75365999 None:intergenic
TTGCCTTTGATTTAATGGAA+AGG 0.533360 4.3:-75365973 MS.gene064109:CDS
TTGAAAGAAGTGATTAGGAA+TGG 0.552785 4.3:+75365760 None:intergenic
ACGTATACAAAGATGGAGAA+GGG 0.557646 4.3:-75366060 MS.gene064109:CDS
AATACAAAATGCATTCGTCA+TGG 0.568013 4.3:-75365811 MS.gene064109:CDS
GACGTATACAAAGATGGAGA+AGG 0.580640 4.3:-75366061 MS.gene064109:CDS
TAGGAATGGCATCAACAGAA+AGG 0.581408 4.3:+75365774 None:intergenic
AATGATGCTCATTGTCACCA+AGG 0.601416 4.3:+75365570 None:intergenic
TTCATATGGACTCTTACAGA+AGG 0.605799 4.3:+75365640 None:intergenic
AGAAGGGGTTCCCTTTAGTG+GGG 0.631108 4.3:-75366044 MS.gene064109:CDS
CGTATACAAAGATGGAGAAG+GGG 0.638066 4.3:-75366059 MS.gene064109:CDS
AATATGTGGAAAGAAATAGA+GGG 0.680535 4.3:-75366103 MS.gene064109:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAAAATGGTGATATTAT+TGG - chr4.3:75365943-75365962 MS.gene064109:CDS 20.0%
!! CCTATATATTCTACAAAAAA+TGG - chr4.3:75365931-75365950 MS.gene064109:CDS 20.0%
!! TAATGATACAACTGTAATAT+GGG - chr4.3:75365825-75365844 MS.gene064109:CDS 20.0%
!! TTAATGATACAACTGTAATA+TGG - chr4.3:75365824-75365843 MS.gene064109:CDS 20.0%
!!! CCATTTTTTGTAGAATATAT+AGG + chr4.3:75365934-75365953 None:intergenic 20.0%
! ATGATAAAGGACAACTATTA+TGG - chr4.3:75365998-75366017 MS.gene064109:CDS 25.0%
! GATATTATTGGAAGAAATCT+TGG - chr4.3:75365955-75365974 MS.gene064109:CDS 25.0%
! TATATTGACGTATACAAAGA+TGG - chr4.3:75365601-75365620 MS.gene064109:CDS 25.0%
! TTGGTGAAGTATAATGATAA+AGG - chr4.3:75365985-75366004 MS.gene064109:CDS 25.0%
! TTTCCTTTCCATTAAATCAA+AGG + chr4.3:75365701-75365720 None:intergenic 25.0%
!! ATTTCTATTTGATGGTTTGT+GGG - chr4.3:75365762-75365781 MS.gene064109:CDS 25.0%
!!! AAGAAAGCATTTCTATTTGA+TGG - chr4.3:75365754-75365773 MS.gene064109:CDS 25.0%
AAGAAATCTTGGTACTAGAT+TGG - chr4.3:75365966-75365985 MS.gene064109:CDS 30.0%
AATACAAAATGCATTCGTCA+TGG - chr4.3:75365857-75365876 MS.gene064109:CDS 30.0%
GCTTTCTTGTCAAAATCATT+TGG + chr4.3:75365742-75365761 None:intergenic 30.0%
GTATACATGCCAATTTCATA+TGG + chr4.3:75366045-75366064 None:intergenic 30.0%
TGACATCTAAACGTAAATCA+TGG + chr4.3:75365672-75365691 None:intergenic 30.0%
TGGAGAATTTCTAAGTCTAT+GGG - chr4.3:75365789-75365808 MS.gene064109:CDS 30.0%
TGTAAGAGTCCATATGAAAT+TGG - chr4.3:75366033-75366052 MS.gene064109:CDS 30.0%
TTGAAAGAAGTGATTAGGAA+TGG + chr4.3:75365911-75365930 None:intergenic 30.0%
TTGGAGAATTTCTAAGTCTA+TGG - chr4.3:75365788-75365807 MS.gene064109:CDS 30.0%
! ATAGGTTGAAAGAAGTGATT+AGG + chr4.3:75365916-75365935 None:intergenic 30.0%
! CATTGTTGCCTTTGATTTAA+TGG - chr4.3:75365690-75365709 MS.gene064109:CDS 30.0%
! CATTTCTATTTGATGGTTTG+TGG - chr4.3:75365761-75365780 MS.gene064109:CDS 30.0%
! TTGCCTTTGATTTAATGGAA+AGG - chr4.3:75365695-75365714 MS.gene064109:CDS 30.0%
! TTGTCAAAATCATTTGGAAC+TGG + chr4.3:75365736-75365755 None:intergenic 30.0%
ACGTATACAAAGATGGAGAA+GGG - chr4.3:75365608-75365627 MS.gene064109:CDS 35.0%
TTCATATGGACTCTTACAGA+AGG + chr4.3:75366031-75366050 None:intergenic 35.0%
! ATTTCTAAGTCTATGGGCTA+TGG - chr4.3:75365795-75365814 MS.gene064109:CDS 35.0%
! TTTGATGGTTTGTGGGTATT+TGG - chr4.3:75365769-75365788 MS.gene064109:CDS 35.0%
AATGATGCTCATTGTCACCA+AGG + chr4.3:75366101-75366120 None:intergenic 40.0%
CGTATACAAAGATGGAGAAG+GGG - chr4.3:75365609-75365628 MS.gene064109:CDS 40.0%
GACGTATACAAAGATGGAGA+AGG - chr4.3:75365607-75365626 MS.gene064109:CDS 40.0%
TAGGAATGGCATCAACAGAA+AGG + chr4.3:75365897-75365916 None:intergenic 40.0%
CAATGAACAGCCCCACTAAA+GGG + chr4.3:75365637-75365656 None:intergenic 45.0%
AGAAGGGGTTCCCTTTAGTG+GGG - chr4.3:75365624-75365643 MS.gene064109:CDS 50.0%
CCAATGAACAGCCCCACTAA+AGG + chr4.3:75365638-75365657 None:intergenic 50.0%
CCTTTAGTGGGGCTGTTCAT+TGG - chr4.3:75365635-75365654 MS.gene064109:CDS 50.0%
GAGAAGGGGTTCCCTTTAGT+GGG - chr4.3:75365623-75365642 MS.gene064109:CDS 50.0%
GAGTCTCTGCTTTCACTCCT+TGG - chr4.3:75366081-75366100 MS.gene064109:CDS 50.0%
TAGTGGGGCTGTTCATTGGT+TGG - chr4.3:75365639-75365658 MS.gene064109:CDS 50.0%
GGAGAAGGGGTTCCCTTTAG+TGG - chr4.3:75365622-75365641 MS.gene064109:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 75365568 75366122 75365568 ID=MS.gene064109
chr4.3 mRNA 75365568 75366122 75365568 ID=MS.gene064109.t1;Parent=MS.gene064109
chr4.3 exon 75365568 75366122 75365568 ID=MS.gene064109.t1.exon1;Parent=MS.gene064109.t1
chr4.3 CDS 75365568 75366122 75365568 ID=cds.MS.gene064109.t1;Parent=MS.gene064109.t1
Gene Sequence

>MS.gene064109

ATGTGGAAAGAAATAGAGGGCACTCACGTCTCTTATATTGACGTATACAAAGATGGAGAAGGGGTTCCCTTTAGTGGGGCTGTTCATTGGTTGGCTTTACGCCATGATTTACGTTTAGATGTCATTGTTGCCTTTGATTTAATGGAAAGGAAATTATTTGAGATGCCAGTTCCAAATGATTTTGACAAGAAAGCATTTCTATTTGATGGTTTGTGGGTATTTGGAGAATTTCTAAGTCTATGGGCTATGGATAATGTTAATGATACAACTGTAATATGGGTGATGAAAGAATACAAAATGCATTCGTCATGGACTAAAACTCTTGTCCTTTCTGTTGATGCCATTCCTAATCACTTCTTTCAACCTATATATTCTACAAAAAATGGTGATATTATTGGAAGAAATCTTGGTACTAGATTGGTGAAGTATAATGATAAAGGACAACTATTATGGCAGCGCTCCTTCTGTAAGAGTCCATATGAAATTGGCATGTATACAGTTGTCATATATACAGAGTCTCTGCTTTCACTCCTTGGTGACAATGAGCATCATTAA

Protein sequence

>MS.gene064109.t1

MWKEIEGTHVSYIDVYKDGEGVPFSGAVHWLALRHDLRLDVIVAFDLMERKLFEMPVPNDFDKKAFLFDGLWVFGEFLSLWAMDNVNDTTVIWVMKEYKMHSSWTKTLVLSVDAIPNHFFQPIYSTKNGDIIGRNLGTRLVKYNDKGQLLWQRSFCKSPYEIGMYTVVIYTESLLSLLGDNEHH