AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50307

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50307.t1 MTR_4g023090 64.407 295 59 5 1 252 95 386 1.03e-124 359
MS.gene50307.t1 MTR_4g023380 65.614 285 65 4 1 252 95 379 1.29e-124 359
MS.gene50307.t1 MTR_4g023080 66.421 271 67 3 1 251 87 353 5.61e-121 349
MS.gene50307.t1 MTR_4g023130 62.593 270 77 3 2 251 47 312 3.82e-111 322
MS.gene50307.t1 MTR_4g021785 60.432 278 71 3 2 251 88 354 3.39e-109 319
MS.gene50307.t1 MTR_4g023070 54.452 292 87 5 2 251 82 369 2.28e-98 292
MS.gene50307.t1 MTR_4g023120 53.925 293 89 5 2 252 82 370 2.13e-97 290
MS.gene50307.t1 MTR_3g103020 54.255 282 94 5 2 251 99 377 1.61e-90 272
MS.gene50307.t1 MTR_4g023440 53.957 278 59 4 1 252 98 332 1.77e-88 265
MS.gene50307.t1 MTR_4g109270 52.158 278 105 5 1 251 87 363 7.24e-88 265
MS.gene50307.t1 MTR_4g023190 52.747 273 101 6 2 250 89 357 6.97e-87 262
MS.gene50307.t1 MTR_4g023150 52.143 280 93 6 2 250 89 358 1.29e-86 261
MS.gene50307.t1 MTR_4g023200 52.015 273 104 6 2 250 89 358 5.85e-85 257
MS.gene50307.t1 MTR_4g122850 51.916 287 100 9 1 252 81 364 2.59e-83 254
MS.gene50307.t1 MTR_4g117720 50.709 282 99 7 7 251 99 377 1.47e-80 247
MS.gene50307.t1 MTR_3g034180 49.315 292 103 7 1 252 203 489 1.51e-79 248
MS.gene50307.t1 MTR_6g060650 49.438 267 104 4 14 251 108 372 9.08e-79 242
MS.gene50307.t1 MTR_4g094365 49.110 281 112 6 1 252 86 364 1.01e-78 241
MS.gene50307.t1 MTR_4g117770 50.000 282 109 5 1 252 88 367 7.67e-78 239
MS.gene50307.t1 MTR_4g117810 50.000 282 109 5 1 252 88 367 7.67e-78 239
MS.gene50307.t1 MTR_4g117780 49.645 282 110 5 1 252 88 367 1.83e-76 236
MS.gene50307.t1 MTR_3g034160 49.655 290 103 6 1 251 89 374 3.96e-76 235
MS.gene50307.t1 MTR_4g116330 49.430 263 100 6 18 251 121 379 4.79e-74 230
MS.gene50307.t1 MTR_4g117850 49.077 271 96 7 11 245 109 373 3.45e-73 228
MS.gene50307.t1 MTR_8g433040 49.265 272 111 5 4 252 97 364 6.03e-73 227
MS.gene50307.t1 MTR_8g032380 49.265 272 111 5 4 252 97 364 6.03e-73 227
MS.gene50307.t1 MTR_5g070130 44.251 287 113 5 1 246 90 370 2.93e-72 226
MS.gene50307.t1 MTR_3g034130 48.507 268 96 5 1 230 89 352 5.57e-72 224
MS.gene50307.t1 MTR_8g032390 50.000 260 97 6 19 247 113 370 1.43e-71 223
MS.gene50307.t1 MTR_4g117870 47.287 258 112 8 2 252 27 267 6.07e-69 213
MS.gene50307.t1 MTR_4g109280 46.831 284 111 6 1 251 82 358 1.63e-68 215
MS.gene50307.t1 MTR_4g118380 46.063 254 97 4 11 227 107 357 1.84e-65 207
MS.gene50307.t1 MTR_5g070080 46.545 275 109 8 10 251 116 385 3.27e-65 207
MS.gene50307.t1 MTR_1g055195 46.454 282 110 9 1 251 206 477 3.75e-65 210
MS.gene50307.t1 MTR_5g097210 45.583 283 109 8 1 248 87 359 1.35e-64 206
MS.gene50307.t1 MTR_1g055135 43.643 291 116 8 1 251 92 374 5.37e-64 204
MS.gene50307.t1 MTR_5g070088 44.484 281 115 8 8 252 111 386 9.38e-63 201
MS.gene50307.t1 MTR_8g040935 61.818 165 56 3 94 251 43 207 2.58e-61 192
MS.gene50307.t1 MTR_4g116320 53.488 215 65 5 68 249 43 255 4.25e-60 191
MS.gene50307.t1 MTR_8g433030 50.450 222 77 6 57 247 118 337 3.07e-58 188
MS.gene50307.t1 MTR_1g055165 43.750 256 111 8 1 251 90 317 3.31e-56 182
MS.gene50307.t1 MTR_4g116330 46.639 238 105 4 18 251 121 340 7.89e-56 182
MS.gene50307.t1 MTR_4g118400 42.857 266 118 6 1 246 43 294 2.16e-54 177
MS.gene50307.t1 MTR_4g117750 42.561 289 114 8 5 251 80 358 1.41e-52 177
MS.gene50307.t1 MTR_4g117750 54.545 132 49 2 130 250 350 481 5.35e-34 128
MS.gene50307.t1 MTR_6g463430 56.471 170 65 3 87 252 38 202 9.78e-52 167
MS.gene50307.t1 MTR_7g117720 43.254 252 83 6 4 247 73 272 5.85e-51 167
MS.gene50307.t1 MTR_4g121580 41.481 270 95 9 7 242 99 339 1.46e-50 170
MS.gene50307.t1 MTR_4g117530 43.404 235 81 5 39 251 48 252 6.43e-48 159
MS.gene50307.t1 MTR_7g079370 38.267 277 123 6 15 247 79 351 4.13e-46 157
MS.gene50307.t1 MTR_4g118370 42.553 235 107 10 33 249 33 257 6.82e-46 154
MS.gene50307.t1 MTR_7g079400 38.462 286 126 9 7 251 60 336 1.84e-45 155
MS.gene50307.t1 MTR_4g114700 43.966 232 107 4 17 243 5 218 1.86e-44 150
MS.gene50307.t1 MTR_1g055175 49.686 159 72 5 98 251 45 200 1.19e-41 141
MS.gene50307.t1 MTR_5g023000 68.367 98 29 1 156 251 1 98 2.53e-39 132
MS.gene50307.t1 MTR_7g079420 38.710 248 127 10 20 251 905 1143 5.10e-37 138
MS.gene50307.t1 MTR_1g055250 39.151 212 93 6 60 239 46 253 1.35e-36 132
MS.gene50307.t1 MTR_8g102520 34.884 258 123 7 31 251 72 321 9.32e-35 127
MS.gene50307.t1 MTR_4g118360 60.714 112 36 4 144 251 18 125 1.49e-34 120
MS.gene50307.t1 MTR_8g013010 40.556 180 76 3 1 153 134 309 1.16e-31 119
MS.gene50307.t1 MTR_4g117430 44.828 145 57 3 32 156 14 155 2.21e-31 114
MS.gene50307.t1 MTR_2g048580 33.692 279 134 11 1 251 86 341 2.55e-30 115
MS.gene50307.t1 MTR_4g023430 51.240 121 42 2 137 252 114 222 6.38e-30 112
MS.gene50307.t1 MTR_7g079450 46.429 140 67 4 115 251 2 136 8.08e-29 106
MS.gene50307.t1 MTR_4g122860 46.094 128 59 4 132 252 196 320 3.66e-24 99.4
MS.gene50307.t1 MTR_4g023100 81.818 55 10 0 198 252 65 119 2.99e-23 91.7
MS.gene50307.t1 MTR_4g116340 39.189 148 61 2 2 120 100 247 1.00e-21 90.9
MS.gene50307.t1 MTR_2g028130 28.169 284 145 13 18 252 106 379 6.40e-21 91.3
MS.gene50307.t1 MTR_8g098830 36.000 175 100 7 77 246 48 215 8.16e-21 88.2
MS.gene50307.t1 MTR_4g122840 43.382 136 38 5 121 252 164 264 4.18e-19 84.3
MS.gene50307.t1 MTR_8g012090 29.730 222 131 8 12 217 119 331 5.91e-19 84.7
MS.gene50307.t1 MTR_0667s0010 26.496 234 123 7 3 188 79 311 8.25e-18 82.4
MS.gene50307.t1 MTR_2g025960 27.468 233 120 7 18 202 5 236 2.02e-17 80.5
MS.gene50307.t1 MTR_2g425980 27.682 289 153 11 18 251 98 385 3.60e-17 80.5
MS.gene50307.t1 MTR_1g068875 25.726 241 141 7 1 205 76 314 5.43e-17 79.7
MS.gene50307.t1 MTR_1g097490 28.000 275 149 13 3 244 132 390 5.48e-17 79.7
MS.gene50307.t1 MTR_1g055200 56.061 66 24 1 17 82 5 65 5.91e-17 74.7
MS.gene50307.t1 MTR_8g013005 66.071 56 17 1 198 251 3 58 8.40e-17 72.8
MS.gene50307.t1 MTR_2g426070 27.719 285 152 12 18 251 105 386 1.11e-16 79.0
MS.gene50307.t1 MTR_4g133170 26.337 243 138 9 14 217 86 326 5.72e-16 77.0
MS.gene50307.t1 MTR_1g068835 24.390 246 148 7 14 221 87 332 8.22e-16 76.3
MS.gene50307.t1 MTR_1g097560 27.206 272 151 16 3 244 119 373 2.59e-15 75.1
MS.gene50307.t1 MTR_2g026010 25.965 285 160 11 14 249 86 368 7.30e-15 73.6
MS.gene50307.t1 MTR_2g028160 27.778 234 119 10 18 202 106 338 8.82e-15 73.6
MS.gene50307.t1 MTR_2g031680 23.843 281 165 9 14 249 87 363 9.05e-15 73.6
MS.gene50307.t1 MTR_2g025420 25.623 281 144 11 18 242 106 377 6.55e-14 70.9
MS.gene50307.t1 MTR_3g036290 27.778 252 147 10 16 244 117 356 1.03e-13 70.1
MS.gene50307.t1 MTR_5g037680 25.191 262 155 10 18 244 126 381 1.84e-13 69.7
MS.gene50307.t1 MTR_8g064790 25.217 230 145 8 21 242 120 330 2.40e-13 68.9
MS.gene50307.t1 MTR_2g024100 25.292 257 136 10 3 205 81 335 3.02e-13 68.9
MS.gene50307.t1 MTR_1g094210 24.280 243 145 8 16 230 152 383 4.90e-13 68.6
MS.gene50307.t1 MTR_4g006270 29.412 204 119 10 14 195 85 285 6.38e-13 68.2
MS.gene50307.t1 MTR_1g094210 24.806 258 154 9 16 244 152 398 7.33e-13 67.8
MS.gene50307.t1 MTR_7g111700 27.907 258 159 10 12 249 115 365 3.00e-12 65.9
MS.gene50307.t1 MTR_3g083640 25.490 255 152 12 19 244 157 402 3.06e-12 66.2
MS.gene50307.t1 MTR_4g107820 28.102 274 138 13 14 244 128 385 7.03e-12 65.1
MS.gene50307.t1 MTR_4g107780 28.102 274 138 13 14 244 128 385 7.03e-12 65.1
MS.gene50307.t1 MTR_4g109290 65.385 52 16 1 202 251 136 187 7.74e-12 62.8
MS.gene50307.t1 MTR_4g107780 28.102 274 138 13 14 244 128 385 9.37e-12 64.7
MS.gene50307.t1 MTR_4g107820 28.102 274 138 13 14 244 128 385 9.46e-12 64.7
MS.gene50307.t1 MTR_8g101580 23.735 257 162 8 19 244 141 394 1.15e-11 64.3
MS.gene50307.t1 MTR_1g068860 23.774 265 160 8 1 225 76 338 1.20e-11 64.3
MS.gene50307.t1 MTR_1g055145 41.584 101 49 2 70 170 112 202 1.25e-11 63.5
MS.gene50307.t1 MTR_1g068905 28.659 164 94 5 1 146 76 234 3.78e-11 62.4
MS.gene50307.t1 MTR_8g099460 29.299 157 77 7 56 183 150 301 4.22e-11 62.8
MS.gene50307.t1 MTR_2g064960 68.293 41 11 1 213 251 18 58 9.13e-11 56.6
MS.gene50307.t1 MTR_5g057710 29.808 208 101 13 18 192 142 337 9.92e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50307.t1 AT3G06240 29.004 231 132 8 14 217 163 388 2.64e-15 75.1
MS.gene50307.t1 AT4G12560 25.086 291 167 9 13 252 88 378 1.07e-13 70.5
MS.gene50307.t1 AT4G12560 25.086 291 167 9 13 252 88 378 1.07e-13 70.5
MS.gene50307.t1 AT4G12560 25.086 291 167 9 13 252 88 378 1.07e-13 70.5

Find 45 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGACAATGTGCTGCTAATT+TGG 0.151154 4.4:+77677153 None:intergenic
ATTTCTAGTTTGTGGGTATT+TGG 0.209175 4.4:-77676797 MS.gene50307:CDS
TTCGCTTCGCATCGTGATTT+AGG 0.254867 4.4:-77676902 MS.gene50307:CDS
ATGATAAAGGACAGTTATTA+AGG 0.300794 4.4:-77676568 MS.gene50307:CDS
CGTATTCATATACATGGTTT+TGG 0.310495 4.4:-77677133 MS.gene50307:CDS
GAAAAGGGTTGCTATTTGAT+AGG 0.322095 4.4:-77676937 MS.gene50307:CDS
ATAATGATACAGTTGAAATA+TGG 0.329909 4.4:-77676742 MS.gene50307:CDS
GCTTCCCGAATCTTATTTGC+CGG 0.352035 4.4:-77677290 MS.gene50307:CDS
TTCAACCGGCAAATAAGATT+CGG 0.360291 4.4:+77677285 None:intergenic
ATGTAAATATTTCTAGTTTG+TGG 0.375634 4.4:-77676805 MS.gene50307:CDS
GGATTCCAAATGTAGATGTT+TGG 0.390948 4.4:+77677220 None:intergenic
ATCCGAAGTTGTCGTGTATA+CGG 0.397437 4.4:-77676522 MS.gene50307:CDS
CCAAGTAATCATCTCTTAAC+TGG 0.398912 4.4:+77677104 None:intergenic
TATTTGCCGGTTGAAATTAG+AGG 0.404868 4.4:-77677277 MS.gene50307:CDS
TGTAAATATTTCTAGTTTGT+GGG 0.407198 4.4:-77676804 MS.gene50307:CDS
CATTGTCGTATTCATATACA+TGG 0.424808 4.4:-77677139 MS.gene50307:CDS
TCAACCGGCAAATAAGATTC+GGG 0.425629 4.4:+77677286 None:intergenic
GTCATCCAAACATCTACATT+TGG 0.429842 4.4:-77677225 MS.gene50307:CDS
TATTTGATAGGGCTATTCAT+TGG 0.433166 4.4:-77676925 MS.gene50307:CDS
CTTCGCATCGTGATTTAGGT+TGG 0.442618 4.4:-77676898 MS.gene50307:CDS
ATTAGCTACAGTTAGATCAT+AGG 0.451354 4.4:+77677069 None:intergenic
CATGAACCTCTAATTTCAAC+CGG 0.456067 4.4:+77677271 None:intergenic
CCAGTTAAGAGATGATTACT+TGG 0.468395 4.4:-77677104 MS.gene50307:CDS
TTGGTGAAGTATAATGATAA+AGG 0.492510 4.4:-77676581 MS.gene50307:CDS
TTTCCTTTCGATTAAATCGA+AGG 0.493566 4.4:+77676862 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.494278 4.4:-77676709 MS.gene50307:CDS
AAGAAATCGTGGTACTAGAT+TGG 0.508666 4.4:-77676600 MS.gene50307:CDS
TACATTTGGAATCCATGCAC+TGG 0.509434 4.4:-77677211 MS.gene50307:CDS
TAAATTTGCTCATTGTCACT+AGG 0.515439 4.4:+77676482 None:intergenic
AAATTTGCTCATTGTCACTA+GGG 0.519922 4.4:+77676483 None:intergenic
AAAAGGGTTGCTATTTGATA+GGG 0.522281 4.4:-77676936 MS.gene50307:CDS
AAATTAGAGGTTCATGTAGA+GGG 0.527818 4.4:-77677264 MS.gene50307:CDS
TAATGATACAGTTGAAATAT+GGG 0.533072 4.4:-77676741 MS.gene50307:CDS
GAAATTAGAGGTTCATGTAG+AGG 0.536201 4.4:-77677265 MS.gene50307:CDS
GCTGCTAATTTGGAACGAAA+AGG 0.541147 4.4:+77677163 None:intergenic
TTGCCTTCGATTTAATCGAA+AGG 0.562789 4.4:-77676865 MS.gene50307:CDS
CTCCGTATACACGACAACTT+CGG 0.564849 4.4:+77676520 None:intergenic
TTCGGATTCACTATTAGAGA+AGG 0.566878 4.4:+77676538 None:intergenic
TTTACATGGTCAATATCACT+TGG 0.571309 4.4:+77676821 None:intergenic
TGGTCAATATCACTTGGAAG+AGG 0.591203 4.4:+77676827 None:intergenic
AAATGTAGATGTTTGGATGA+CGG 0.595423 4.4:+77677227 None:intergenic
GATATTATTGGAAGAAATCG+TGG 0.613647 4.4:-77676611 MS.gene50307:CDS
TTGGAACGAAAAGGAGACAC+AGG 0.637002 4.4:+77677172 None:intergenic
ACAAACTAGAAATATTTACA+TGG 0.657492 4.4:+77676807 None:intergenic
ATAGGTCGGAAGTAGCGACG+AGG 0.788050 4.4:+77676653 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGTAGAAAGAAATTAA+GGG - chr4.4:77676781-77676800 MS.gene50307:CDS 15.0%
!! TAATATGTAGAAAGAAATTA+AGG - chr4.4:77676780-77676799 MS.gene50307:CDS 15.0%
!! ACAAACTAGAAATATTTACA+TGG + chr4.4:77676984-77677003 None:intergenic 20.0%
!! ATAATGATACAGTTGAAATA+TGG - chr4.4:77677046-77677065 MS.gene50307:intron 20.0%
!! TAATGATACAGTTGAAATAT+GGG - chr4.4:77677047-77677066 MS.gene50307:intron 20.0%
!!! ATGTAAATATTTCTAGTTTG+TGG - chr4.4:77676983-77677002 MS.gene50307:intron 20.0%
!!! TGTAAATATTTCTAGTTTGT+GGG - chr4.4:77676984-77677003 MS.gene50307:intron 20.0%
!!! TTTTTGTAGAATATATAGGT+CGG + chr4.4:77677152-77677171 None:intergenic 20.0%
! ACAAAAAGTGGTGATATTAT+TGG - chr4.4:77677165-77677184 MS.gene50307:CDS 25.0%
! ATGATAAAGGACAGTTATTA+AGG - chr4.4:77677220-77677239 MS.gene50307:CDS 25.0%
! CCTATATATTCTACAAAAAG+TGG - chr4.4:77677153-77677172 MS.gene50307:CDS 25.0%
! TTGGTGAAGTATAATGATAA+AGG - chr4.4:77677207-77677226 MS.gene50307:CDS 25.0%
!!! CCACTTTTTGTAGAATATAT+AGG + chr4.4:77677156-77677175 None:intergenic 25.0%
AAATGTAGATGTTTGGATGA+CGG + chr4.4:77676564-77676583 None:intergenic 30.0%
AAATTAGAGGTTCATGTAGA+GGG - chr4.4:77676524-77676543 MS.gene50307:CDS 30.0%
ATTAGCTACAGTTAGATCAT+AGG + chr4.4:77676722-77676741 None:intergenic 30.0%
CATTGTCGTATTCATATACA+TGG - chr4.4:77676649-77676668 MS.gene50307:CDS 30.0%
GATATTATTGGAAGAAATCG+TGG - chr4.4:77677177-77677196 MS.gene50307:CDS 30.0%
TATTTGATAGGGCTATTCAT+TGG - chr4.4:77676863-77676882 MS.gene50307:CDS 30.0%
TTTACATGGTCAATATCACT+TGG + chr4.4:77676970-77676989 None:intergenic 30.0%
TTTCCTTTCGATTAAATCGA+AGG + chr4.4:77676929-77676948 None:intergenic 30.0%
! AAATTTGCTCATTGTCACTA+GGG + chr4.4:77677308-77677327 None:intergenic 30.0%
! CGTATTCATATACATGGTTT+TGG - chr4.4:77676655-77676674 MS.gene50307:CDS 30.0%
! TAAATTTGCTCATTGTCACT+AGG + chr4.4:77677309-77677328 None:intergenic 30.0%
!! AAAAGGGTTGCTATTTGATA+GGG - chr4.4:77676852-77676871 MS.gene50307:CDS 30.0%
!! ATTTCTAGTTTGTGGGTATT+TGG - chr4.4:77676991-77677010 MS.gene50307:intron 30.0%
!! TGGAGAATTTTTCAGTCTAT+GGG - chr4.4:77677011-77677030 MS.gene50307:intron 30.0%
!! TTGGAGAATTTTTCAGTCTA+TGG - chr4.4:77677010-77677029 MS.gene50307:intron 30.0%
AAGAAATCGTGGTACTAGAT+TGG - chr4.4:77677188-77677207 MS.gene50307:CDS 35.0%
AATACAAAGTGCATTCGTCT+TGG - chr4.4:77677079-77677098 MS.gene50307:CDS 35.0%
ACAGAATAAGGGAAGTGATA+AGG + chr4.4:77676817-77676836 None:intergenic 35.0%
ATTCGGGAAGCGAAAAATTA+AGG + chr4.4:77676489-77676508 None:intergenic 35.0%
CAGAATAAGGGAAGTGATAA+GGG + chr4.4:77676816-77676835 None:intergenic 35.0%
CATGAACCTCTAATTTCAAC+CGG + chr4.4:77676520-77676539 None:intergenic 35.0%
CCAAGTAATCATCTCTTAAC+TGG + chr4.4:77676687-77676706 None:intergenic 35.0%
CCAGTTAAGAGATGATTACT+TGG - chr4.4:77676684-77676703 MS.gene50307:CDS 35.0%
GAAATTAGAGGTTCATGTAG+AGG - chr4.4:77676523-77676542 MS.gene50307:CDS 35.0%
GGATTCCAAATGTAGATGTT+TGG + chr4.4:77676571-77676590 None:intergenic 35.0%
GTCATCCAAACATCTACATT+TGG - chr4.4:77676563-77676582 MS.gene50307:CDS 35.0%
TTCAACCGGCAAATAAGATT+CGG + chr4.4:77676506-77676525 None:intergenic 35.0%
TTCGGATTCACTATTAGAGA+AGG + chr4.4:77677253-77677272 None:intergenic 35.0%
TTGCCTTCGATTTAATCGAA+AGG - chr4.4:77676923-77676942 MS.gene50307:CDS 35.0%
! TATTTGCCGGTTGAAATTAG+AGG - chr4.4:77676511-77676530 MS.gene50307:CDS 35.0%
!! GAAAAGGGTTGCTATTTGAT+AGG - chr4.4:77676851-77676870 MS.gene50307:CDS 35.0%
!! TGTGTTTTAAATCCAGTGCA+TGG + chr4.4:77676592-77676611 None:intergenic 35.0%
ATCCGAAGTTGTCGTGTATA+CGG - chr4.4:77677266-77677285 MS.gene50307:CDS 40.0%
GACAAGGAACGACAGAATAA+GGG + chr4.4:77676828-77676847 None:intergenic 40.0%
TCAACCGGCAAATAAGATTC+GGG + chr4.4:77676505-77676524 None:intergenic 40.0%
TGGTCAATATCACTTGGAAG+AGG + chr4.4:77676964-77676983 None:intergenic 40.0%
! ACGACAATGTGCTGCTAATT+TGG + chr4.4:77676638-77676657 None:intergenic 40.0%
! GCTGCTAATTTGGAACGAAA+AGG + chr4.4:77676628-77676647 None:intergenic 40.0%
! TACATTTGGAATCCATGCAC+TGG - chr4.4:77676577-77676596 MS.gene50307:CDS 40.0%
CGACAAGGAACGACAGAATA+AGG + chr4.4:77676829-77676848 None:intergenic 45.0%
CTCCGTATACACGACAACTT+CGG + chr4.4:77677271-77677290 None:intergenic 45.0%
CTTCGCATCGTGATTTAGGT+TGG - chr4.4:77676890-77676909 MS.gene50307:CDS 45.0%
TCGTTCCTTGTCGCAGAAAA+GGG - chr4.4:77676836-77676855 MS.gene50307:CDS 45.0%
TTCGCTTCGCATCGTGATTT+AGG - chr4.4:77676886-77676905 MS.gene50307:CDS 45.0%
TTGGAACGAAAAGGAGACAC+AGG + chr4.4:77676619-77676638 None:intergenic 45.0%
! GCTTCCCGAATCTTATTTGC+CGG - chr4.4:77676498-77676517 MS.gene50307:CDS 45.0%
GTCGTTCCTTGTCGCAGAAA+AGG - chr4.4:77676835-77676854 MS.gene50307:CDS 50.0%
! AGCAACCCTTTTCTGCGACA+AGG + chr4.4:77676844-77676863 None:intergenic 50.0%
ATAGGTCGGAAGTAGCGACG+AGG + chr4.4:77677138-77677157 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 77676481 77677329 77676481 ID=MS.gene50307
chr4.4 mRNA 77676481 77677329 77676481 ID=MS.gene50307.t1;Parent=MS.gene50307
chr4.4 exon 77677049 77677329 77677049 ID=MS.gene50307.t1.exon1;Parent=MS.gene50307.t1
chr4.4 CDS 77677049 77677329 77677049 ID=cds.MS.gene50307.t1;Parent=MS.gene50307.t1
chr4.4 exon 77676481 77676958 77676481 ID=MS.gene50307.t1.exon2;Parent=MS.gene50307.t1
chr4.4 CDS 77676481 77676958 77676481 ID=cds.MS.gene50307.t1;Parent=MS.gene50307.t1
Gene Sequence

>MS.gene50307

ATGAACCTTAATTTTTCGCTTCCCGAATCTTATTTGCCGGTTGAAATTAGAGGTTCATGTAGAGGGTTTATACTTTTGTACCGTCATCCAAACATCTACATTTGGAATCCATGCACTGGATTTAAAACACAAATACCTGTGTCTCCTTTTCGTTCCAAATTAGCAGCACATTGTCGTATTCATATACATGGTTTTGGTTATGACCAGTTAAGAGATGATTACTTGGTCGTTTCATTGTCCTATGATCTAACTGTAGCTAATGCTGTTTCAACACATTTGAAGTTTTTCTCATTTAGAGATAATATGTAGAAAGAAATTAAGGGTACTCGCTTCCCTTATCACTTCCCTTATTCTGTCGTTCCTTGTCGCAGAAAAGGGTTGCTATTTGATAGGGCTATTCATTGGTTCGCTTCGCATCGTGATTTAGGTTGGAATGTCATTGTTGCCTTCGATTTAATCGAAAGGAAATTCTTTGAGATGCCTCTTCCAAGTGATATTGACCATGTAAATATTTCTAGTTTGTGGGTATTTGGAGAATTTTTCAGTCTATGGGTTATCGACTATGATAATGATACAGTTGAAATATGGGTGATGAAAGAATACAAAGTGCATTCGTCTTGGACTAAAACTCTTGTTCTTTCTGTTGATGTCGTTCCTCGTCGCTACTTCCGACCTATATATTCTACAAAAAGTGGTGATATTATTGGAAGAAATCGTGGTACTAGATTGGTGAAGTATAATGATAAAGGACAGTTATTAAGGCATCGATCCTTCTCTAATAGTGAATCCGAAGTTGTCGTGTATACGGAGTCTCTGCTTTCACTCCCTAGTGACAATGAGCAAATTTAA
Protein sequence

>MS.gene50307.t1

MNLNFSLPESYLPVEIRGSCRGFILLYRHPNIYIWNPCTGFKTQIPVSPFRSKLAAHCRIHIHGFGYDQLRDDYLVVSLSYDLTVANAVSTHLKKGLLFDRAIHWFASHRDLGWNVIVAFDLIERKFFEMPLPSDIDHVNISSLWVFGEFFSLWVIDYDNDTVEIWVMKEYKVHSSWTKTLVLSVDVVPRRYFRPIYSTKSGDIIGRNRGTRLVKYNDKGQLLRHRSFSNSESEVVVYTESLLSLPSDNEQI