AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39262


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39262.t1 MTR_1g055175 77.612 134 28 1 1 134 70 201 1.76e-68 205
MS.gene39262.t1 MTR_1g055165 77.612 134 28 1 1 134 187 318 1.93e-67 206
MS.gene39262.t1 MTR_1g055135 69.118 136 39 2 1 134 241 375 6.63e-55 176
MS.gene39262.t1 MTR_1g055195 63.433 134 46 3 1 134 348 478 3.67e-48 161
MS.gene39262.t1 MTR_1g055250 66.355 107 33 2 2 108 140 243 1.50e-41 140
MS.gene39262.t1 MTR_5g097210 60.606 132 42 4 2 130 235 359 2.47e-40 138
MS.gene39262.t1 MTR_4g118370 55.725 131 53 4 1 131 132 257 3.16e-39 132
MS.gene39262.t1 MTR_4g109280 57.353 136 52 5 1 134 228 359 6.10e-38 132
MS.gene39262.t1 MTR_4g117720 55.319 141 52 7 1 134 242 378 3.84e-37 130
MS.gene39262.t1 MTR_4g094365 50.735 136 63 3 1 134 231 364 7.39e-34 121
MS.gene39262.t1 MTR_6g060650 50.000 134 62 4 1 133 243 372 5.91e-33 119
MS.gene39262.t1 MTR_3g103020 54.015 137 56 5 1 134 246 378 1.13e-32 118
MS.gene39262.t1 MTR_4g118360 48.438 128 63 3 8 134 1 126 1.53e-32 111
MS.gene39262.t1 MTR_8g433040 48.148 135 64 3 1 134 235 364 3.34e-31 114
MS.gene39262.t1 MTR_8g032380 48.148 135 64 3 1 134 235 364 3.34e-31 114
MS.gene39262.t1 MTR_6g463430 48.889 135 62 4 1 134 74 202 3.42e-31 110
MS.gene39262.t1 MTR_4g117870 47.692 130 65 2 5 134 141 267 4.82e-31 112
MS.gene39262.t1 MTR_4g117750 52.941 119 53 3 12 130 240 355 5.61e-31 115
MS.gene39262.t1 MTR_4g117750 50.909 110 51 3 25 132 373 481 1.13e-24 97.8
MS.gene39262.t1 MTR_4g116320 50.365 137 55 5 1 131 126 255 5.84e-31 111
MS.gene39262.t1 MTR_4g116330 48.921 139 62 6 1 134 246 380 3.19e-30 112
MS.gene39262.t1 MTR_3g034180 50.746 134 60 5 1 133 360 488 6.57e-30 112
MS.gene39262.t1 MTR_4g109270 49.265 136 64 4 1 134 232 364 1.85e-29 109
MS.gene39262.t1 MTR_8g040935 50.725 138 59 5 2 134 75 208 2.02e-29 106
MS.gene39262.t1 MTR_8g433030 51.538 130 56 5 1 128 212 336 2.79e-29 108
MS.gene39262.t1 MTR_8g032390 51.538 130 56 5 1 128 245 369 7.50e-29 108
MS.gene39262.t1 MTR_4g117850 51.163 129 57 4 2 127 248 373 1.44e-28 107
MS.gene39262.t1 MTR_4g122850 46.324 136 68 4 1 134 232 364 3.14e-28 106
MS.gene39262.t1 MTR_4g023380 43.125 160 60 8 1 134 225 379 1.56e-27 104
MS.gene39262.t1 MTR_4g021785 45.588 136 66 5 1 134 226 355 1.34e-26 102
MS.gene39262.t1 MTR_4g023070 45.890 146 61 6 2 133 228 369 1.78e-26 102
MS.gene39262.t1 MTR_4g118380 51.786 112 45 3 1 104 245 355 1.94e-26 101
MS.gene39262.t1 MTR_5g070130 49.618 131 60 5 1 128 243 370 3.45e-26 101
MS.gene39262.t1 MTR_4g023130 47.794 136 61 7 1 133 184 312 7.06e-26 99.4
MS.gene39262.t1 MTR_5g070080 50.000 136 61 6 2 134 255 386 8.96e-26 100
MS.gene39262.t1 MTR_4g023080 47.794 136 61 7 1 133 225 353 1.11e-25 99.4
MS.gene39262.t1 MTR_4g117530 55.319 94 35 3 43 134 165 253 1.50e-25 97.4
MS.gene39262.t1 MTR_5g070088 46.565 131 64 5 1 128 252 379 9.17e-25 97.4
MS.gene39262.t1 MTR_4g023190 46.377 138 63 6 1 132 225 357 9.38e-25 97.1
MS.gene39262.t1 MTR_5g023000 56.250 96 34 5 43 134 8 99 1.11e-24 90.9
MS.gene39262.t1 MTR_7g079400 43.796 137 71 3 2 134 203 337 1.25e-24 96.3
MS.gene39262.t1 MTR_4g118400 48.462 130 46 6 2 128 183 294 1.73e-24 95.5
MS.gene39262.t1 MTR_4g023120 42.667 150 62 6 2 134 228 370 6.19e-24 95.1
MS.gene39262.t1 MTR_4g117780 48.214 112 55 3 24 133 256 366 7.38e-24 94.7
MS.gene39262.t1 MTR_4g117770 47.788 113 56 3 24 134 256 367 8.51e-24 94.7
MS.gene39262.t1 MTR_4g117810 47.788 113 56 3 24 134 256 367 8.51e-24 94.7
MS.gene39262.t1 MTR_4g023150 44.928 138 65 6 1 132 226 358 1.26e-23 94.0
MS.gene39262.t1 MTR_4g023090 41.060 151 66 8 1 133 240 385 3.46e-23 93.2
MS.gene39262.t1 MTR_8g102520 42.222 135 72 4 2 134 192 322 3.59e-22 89.7
MS.gene39262.t1 MTR_7g079450 40.602 133 73 3 2 134 11 137 4.53e-22 85.5
MS.gene39262.t1 MTR_4g023200 43.478 138 67 6 1 132 226 358 6.48e-22 89.4
MS.gene39262.t1 MTR_3g034160 47.015 134 65 4 1 133 246 374 6.93e-22 89.4
MS.gene39262.t1 MTR_4g116330 50.000 114 49 6 27 134 230 341 1.04e-21 88.6
MS.gene39262.t1 MTR_3g034130 45.238 126 54 5 1 120 246 362 1.37e-21 88.6
MS.gene39262.t1 MTR_7g079420 41.481 135 74 4 2 134 1013 1144 3.31e-21 88.6
MS.gene39262.t1 MTR_4g122860 43.651 126 61 4 14 133 198 319 5.01e-21 86.7
MS.gene39262.t1 MTR_4g023440 51.042 96 41 4 40 133 240 331 1.26e-20 85.5
MS.gene39262.t1 MTR_7g079370 43.182 132 64 7 2 129 227 351 3.58e-18 79.3
MS.gene39262.t1 MTR_7g117720 37.956 137 63 6 11 129 140 272 7.56e-15 69.3
MS.gene39262.t1 MTR_8g013005 55.000 60 26 1 75 134 1 59 7.80e-14 62.0
MS.gene39262.t1 MTR_4g114700 43.182 88 47 2 41 125 131 218 1.38e-13 65.5
MS.gene39262.t1 MTR_4g023100 45.455 66 32 2 69 133 56 118 8.84e-13 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCACAGAATCTAATTTA+TGG 0.185039 1.1:+43103491 MS.gene39262:CDS
ACAAGGGTAAAGTTGATATA+TGG 0.197793 1.1:+43103551 MS.gene39262:CDS
ATTTATCAGTCTATGGGTTT+GGG 0.253367 1.1:+43103525 MS.gene39262:CDS
TTAGAGGATTTATCAGTCTA+TGG 0.298080 1.1:+43103518 MS.gene39262:CDS
CATTCAAAATCATCGTCATT+TGG 0.304453 1.1:-43103466 None:intergenic
TTTCACAGAATCTAATTTAT+GGG 0.336216 1.1:+43103492 MS.gene39262:CDS
GATTTATCAGTCTATGGGTT+TGG 0.343864 1.1:+43103524 MS.gene39262:CDS
ATTCAAAATCATCGTCATTT+GGG 0.346028 1.1:-43103465 None:intergenic
TCTAATTTATGGGTATTTAG+AGG 0.356771 1.1:+43103502 MS.gene39262:CDS
AATACAAAGTGCAGTCATCT+TGG 0.424763 1.1:+43103584 MS.gene39262:CDS
CAAGGGTAAAGTTGATATAT+GGG 0.454916 1.1:+43103552 MS.gene39262:CDS
TTGCCTTTCATTTAATGGAA+AGG 0.488920 1.1:+43103422 None:intergenic
TAGAGGATTTATCAGTCTAT+GGG 0.499656 1.1:+43103519 MS.gene39262:CDS
TAAGCTTCCTCATTGTCACT+AGG 0.504127 1.1:-43103817 None:intergenic
GTTGGAACAGATTACCGTAC+TGG 0.514745 1.1:+43103685 MS.gene39262:CDS
TACAAGGGCAACGTTATGAA+TGG 0.537235 1.1:-43103638 None:intergenic
AAATCATCGTCATTTGGGAG+AGG 0.549721 1.1:-43103460 None:intergenic
TTATCAGTCTATGGGTTTGG+GGG 0.551494 1.1:+43103527 MS.gene39262:CDS
AAGCTTCCTCATTGTCACTA+GGG 0.552135 1.1:-43103816 None:intergenic
TTCACGAAATGAATTCCGAT+TGG 0.558524 1.1:+43103765 MS.gene39262:CDS
TTTATCAGTCTATGGGTTTG+GGG 0.570897 1.1:+43103526 MS.gene39262:CDS
GAACAGATTACCGTACTGGA+TGG 0.571251 1.1:+43103689 MS.gene39262:CDS
ACTCTGTATACATAGCCAAT+CGG 0.573189 1.1:-43103780 None:intergenic
CATATCTCACCCATCCAGTA+CGG 0.584321 1.1:-43103699 None:intergenic
AACAGATTACCGTACTGGAT+GGG 0.623237 1.1:+43103690 MS.gene39262:CDS
TTCACTCCCTAGTGACAATG+AGG 0.626833 1.1:+43103810 MS.gene39262:CDS
TGGGTGAGATATGATGACAA+AGG 0.747254 1.1:+43103709 MS.gene39262:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCACAGAATCTAATTTAT+GGG + chr1.1:43103492-43103511 MS.gene39262:CDS 20.0%
! ATTCAAAATCATCGTCATTT+GGG - chr1.1:43103468-43103487 None:intergenic 25.0%
! GTTTCACAGAATCTAATTTA+TGG + chr1.1:43103491-43103510 MS.gene39262:CDS 25.0%
! TCTAATTTATGGGTATTTAG+AGG + chr1.1:43103502-43103521 MS.gene39262:CDS 25.0%
ACAAAAAGTGGTGATATTGT+TGG + chr1.1:43103667-43103686 MS.gene39262:CDS 30.0%
ATTTATCAGTCTATGGGTTT+GGG + chr1.1:43103525-43103544 MS.gene39262:CDS 30.0%
CATTCAAAATCATCGTCATT+TGG - chr1.1:43103469-43103488 None:intergenic 30.0%
TAGAGGATTTATCAGTCTAT+GGG + chr1.1:43103519-43103538 MS.gene39262:CDS 30.0%
TTAGAGGATTTATCAGTCTA+TGG + chr1.1:43103518-43103537 MS.gene39262:CDS 30.0%
! ACTTTTTGTGCAACATACAA+GGG - chr1.1:43103656-43103675 None:intergenic 30.0%
!! ACAAGGGTAAAGTTGATATA+TGG + chr1.1:43103551-43103570 MS.gene39262:CDS 30.0%
!! CAAGGGTAAAGTTGATATAT+GGG + chr1.1:43103552-43103571 MS.gene39262:CDS 30.0%
AATACAAAGTGCAGTCATCT+TGG + chr1.1:43103584-43103603 MS.gene39262:CDS 35.0%
ACTCTGTATACATAGCCAAT+CGG - chr1.1:43103783-43103802 None:intergenic 35.0%
CTTGTATGTTGCACAAAAAG+TGG + chr1.1:43103655-43103674 MS.gene39262:CDS 35.0%
GATTTATCAGTCTATGGGTT+TGG + chr1.1:43103524-43103543 MS.gene39262:CDS 35.0%
TTCACGAAATGAATTCCGAT+TGG + chr1.1:43103765-43103784 MS.gene39262:CDS 35.0%
TTTATCAGTCTATGGGTTTG+GGG + chr1.1:43103526-43103545 MS.gene39262:CDS 35.0%
!! CACTTTTTGTGCAACATACA+AGG - chr1.1:43103657-43103676 None:intergenic 35.0%
AAATCATCGTCATTTGGGAG+AGG - chr1.1:43103463-43103482 None:intergenic 40.0%
AACAGATTACCGTACTGGAT+GGG + chr1.1:43103690-43103709 MS.gene39262:CDS 40.0%
AAGCTTCCTCATTGTCACTA+GGG - chr1.1:43103819-43103838 None:intergenic 40.0%
TAAGCTTCCTCATTGTCACT+AGG - chr1.1:43103820-43103839 None:intergenic 40.0%
TACAAGGGCAACGTTATGAA+TGG - chr1.1:43103641-43103660 None:intergenic 40.0%
TGGGTGAGATATGATGACAA+AGG + chr1.1:43103709-43103728 MS.gene39262:CDS 40.0%
TTATCAGTCTATGGGTTTGG+GGG + chr1.1:43103527-43103546 MS.gene39262:CDS 40.0%
CATATCTCACCCATCCAGTA+CGG - chr1.1:43103702-43103721 None:intergenic 45.0%
GAACAGATTACCGTACTGGA+TGG + chr1.1:43103689-43103708 MS.gene39262:CDS 45.0%
GTTGGAACAGATTACCGTAC+TGG + chr1.1:43103685-43103704 MS.gene39262:CDS 45.0%
TTCACTCCCTAGTGACAATG+AGG + chr1.1:43103810-43103829 MS.gene39262:CDS 45.0%
! CTATGGGTTTGGGGGTACAA+GGG + chr1.1:43103535-43103554 MS.gene39262:CDS 50.0%
! TCTATGGGTTTGGGGGTACA+AGG + chr1.1:43103534-43103553 MS.gene39262:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 43103436 43103840 43103436 ID=MS.gene39262
chr1.1 mRNA 43103436 43103840 43103436 ID=MS.gene39262.t1;Parent=MS.gene39262
chr1.1 exon 43103436 43103840 43103436 ID=MS.gene39262.t1.exon1;Parent=MS.gene39262.t1
chr1.1 CDS 43103436 43103840 43103436 ID=cds.MS.gene39262.t1;Parent=MS.gene39262.t1
Gene Sequence

>MS.gene39262

ATGGAAAGGATACTTCTAGAAATTCCTCTCCCAAATGACGATGATTTTGAATGTAGTTTCACAGAATCTAATTTATGGGTATTTAGAGGATTTATCAGTCTATGGGTTTGGGGGTACAAGGGTAAAGTTGATATATGGGTGATGAAAGAATACAAAGTGCAGTCATCTTGGACTAAAACTCTTGTTCTTATTACTTATGATACCATTCATAACGTTGCCCTTGTATGTTGCACAAAAAGTGGTGATATTGTTGGAACAGATTACCGTACTGGATGGGTGAGATATGATGACAAAGGAGAGTTTCTAGAGCATATCTACTATTGCATAGATTCACGAAATGAATTCCGATTGGCTATGTATACAGAGTCTTTGCTTTCACTCCCTAGTGACAATGAGGAAGCTTAA

Protein sequence

>MS.gene39262.t1

MERILLEIPLPNDDDFECSFTESNLWVFRGFISLWVWGYKGKVDIWVMKEYKVQSSWTKTLVLITYDTIHNVALVCCTKSGDIVGTDYRTGWVRYDDKGEFLEHIYYCIDSRNEFRLAMYTESLLSLPSDNEEA