AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045473

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045473.t1 MTR_4g080200 55.679 361 115 6 1 327 1 350 1.29e-128 371
MS.gene045473.t1 MTR_1g023400 43.631 369 148 11 1 326 1 352 4.60e-82 253
MS.gene045473.t1 MTR_1g009110 43.048 374 156 13 2 326 13 378 1.31e-77 242
MS.gene045473.t1 MTR_1g022200 42.204 372 154 14 1 326 13 369 1.11e-70 224
MS.gene045473.t1 MTR_1g037540 37.531 405 163 18 3 326 5 400 4.31e-56 187
MS.gene045473.t1 MTR_1g022105 35.866 329 163 10 2 310 20 320 2.51e-44 154
MS.gene045473.t1 MTR_8g093280 68.000 100 27 1 7 106 11 105 4.37e-36 126
MS.gene045473.t1 MTR_1g023430 41.007 139 74 4 191 326 39 172 2.70e-24 97.8
MS.gene045473.t1 MTR_1g024125 46.667 90 42 3 222 308 62 148 5.62e-18 80.1
MS.gene045473.t1 MTR_1g008000 31.274 259 148 13 80 326 28 268 7.50e-17 79.3
MS.gene045473.t1 MTR_4g133170 27.094 203 123 5 2 193 3 191 3.45e-16 79.0
MS.gene045473.t1 MTR_3g009310 27.385 325 173 14 6 284 45 352 4.26e-16 79.0
MS.gene045473.t1 MTR_2g028140 28.311 219 122 7 3 193 5 216 1.83e-14 73.9
MS.gene045473.t1 MTR_4g055060 27.542 236 152 8 6 230 33 260 8.47e-14 72.0
MS.gene045473.t1 MTR_7g070440 31.982 222 129 10 5 217 9 217 8.82e-14 71.6
MS.gene045473.t1 MTR_7g013590 28.502 207 116 6 6 197 39 228 9.22e-14 72.0
MS.gene045473.t1 MTR_3g013740 28.713 202 120 8 2 186 43 237 1.11e-13 71.6
MS.gene045473.t1 MTR_4g030760 31.401 207 106 10 3 193 73 259 3.95e-13 70.1
MS.gene045473.t1 MTR_6g090010 71.429 42 12 0 2 43 54 95 4.25e-13 66.6
MS.gene045473.t1 MTR_5g057710 28.261 230 124 9 6 217 49 255 4.82e-13 69.7
MS.gene045473.t1 MTR_3g103020 28.796 191 115 7 6 189 23 199 5.11e-13 69.7
MS.gene045473.t1 MTR_6g004210 26.768 198 130 5 14 197 36 232 5.12e-13 69.7
MS.gene045473.t1 MTR_7g017170 29.187 209 121 8 2 186 17 222 7.60e-13 69.3
MS.gene045473.t1 MTR_7g017130 29.187 209 121 8 2 186 17 222 8.18e-13 68.9
MS.gene045473.t1 MTR_5g010660 24.167 360 216 13 2 325 33 371 2.02e-12 68.2
MS.gene045473.t1 MTR_4g130330 28.085 235 123 11 5 205 9 231 2.08e-12 67.8
MS.gene045473.t1 MTR_5g068040 31.928 166 92 6 3 160 16 168 2.46e-12 67.4
MS.gene045473.t1 MTR_3g014540 28.426 197 131 5 2 193 28 219 2.83e-12 67.4
MS.gene045473.t1 MTR_6g065980 26.861 309 147 14 6 270 19 292 2.88e-12 67.0
MS.gene045473.t1 MTR_0620s0010 64.286 42 15 0 2 43 5 46 2.99e-12 61.6
MS.gene045473.t1 MTR_3g009240 31.088 193 121 6 6 193 39 224 3.25e-12 67.0
MS.gene045473.t1 MTR_8g012090 32.749 171 98 9 5 159 18 187 3.60e-12 66.6
MS.gene045473.t1 MTR_4g129190 27.119 236 147 7 1 227 4 223 3.64e-12 67.0
MS.gene045473.t1 MTR_5g057790 27.273 231 125 9 6 217 49 255 4.13e-12 67.0
MS.gene045473.t1 MTR_5g057020 28.767 219 122 10 6 217 49 240 4.16e-12 66.6
MS.gene045473.t1 MTR_5g057643 27.386 241 134 9 6 228 49 266 4.53e-12 65.9
MS.gene045473.t1 MTR_7g066840 30.500 200 115 7 3 193 23 207 5.32e-12 66.6
MS.gene045473.t1 MTR_8g464770 31.944 216 120 10 2 195 11 221 6.77e-12 66.2
MS.gene045473.t1 MTR_6g055210 27.948 229 153 7 5 223 24 250 7.20e-12 66.2
MS.gene045473.t1 MTR_3g034180 25.000 196 129 5 3 189 123 309 7.39e-12 66.2
MS.gene045473.t1 MTR_8g013010 26.250 240 144 11 1 227 52 271 1.17e-11 65.1
MS.gene045473.t1 MTR_5g074850 28.509 228 120 10 3 195 35 254 1.26e-11 66.2
MS.gene045473.t1 MTR_4g031770 27.136 199 121 8 6 193 7 192 1.33e-11 65.1
MS.gene045473.t1 MTR_4g109280 28.571 133 87 2 2 134 7 131 1.43e-11 65.1
MS.gene045473.t1 MTR_8g073550 30.846 201 103 9 3 186 30 211 1.45e-11 63.9
MS.gene045473.t1 MTR_3g058480 28.079 203 125 6 2 189 38 234 1.53e-11 64.7
MS.gene045473.t1 MTR_3g007270 28.085 235 145 7 6 224 50 276 1.54e-11 65.1
MS.gene045473.t1 MTR_4g117780 32.474 194 109 8 2 189 10 187 1.84e-11 64.7
MS.gene045473.t1 MTR_7g016930 28.571 196 114 8 5 186 18 201 1.89e-11 64.7
MS.gene045473.t1 MTR_1g094210 28.500 200 115 10 3 193 57 237 2.56e-11 64.3
MS.gene045473.t1 MTR_3g083640 22.350 349 219 10 2 312 54 388 2.66e-11 64.3
MS.gene045473.t1 MTR_1g056710 29.817 218 126 11 6 212 37 238 2.89e-11 64.3
MS.gene045473.t1 MTR_1g054575 30.500 200 113 8 3 193 52 234 2.99e-11 64.7
MS.gene045473.t1 MTR_3g007360 29.082 196 125 7 6 193 55 244 3.28e-11 64.3
MS.gene045473.t1 MTR_6g089990 66.667 42 14 0 2 43 54 95 3.55e-11 62.4
MS.gene045473.t1 MTR_5g011200 28.137 263 143 13 6 250 20 254 3.60e-11 63.9
MS.gene045473.t1 MTR_4g019990 25.681 257 146 11 3 231 6 245 3.67e-11 63.9
MS.gene045473.t1 MTR_1g094210 28.500 200 115 10 3 193 57 237 3.68e-11 63.9
MS.gene045473.t1 MTR_3g013770 27.586 203 128 5 3 193 33 228 3.83e-11 63.9
MS.gene045473.t1 MTR_8g046310 27.014 211 111 9 2 186 15 208 3.98e-11 63.9
MS.gene045473.t1 MTR_3g009260 28.571 203 106 6 6 193 44 222 4.06e-11 63.9
MS.gene045473.t1 MTR_5g070130 29.730 222 136 8 3 217 11 219 4.71e-11 63.5
MS.gene045473.t1 MTR_8g467140 28.922 204 112 8 3 193 32 215 5.00e-11 63.2
MS.gene045473.t1 MTR_3g009250 28.079 203 107 6 6 193 44 222 5.28e-11 62.4
MS.gene045473.t1 MTR_3g007410 26.407 231 131 9 6 217 55 265 5.77e-11 63.5
MS.gene045473.t1 MTR_4g117550 26.126 222 129 9 2 193 16 232 6.32e-11 63.2
MS.gene045473.t1 MTR_7g069550 25.778 225 125 10 5 193 16 234 6.70e-11 63.2
MS.gene045473.t1 MTR_3g036430 27.919 197 105 7 2 193 19 183 7.72e-11 62.8
MS.gene045473.t1 MTR_3g006530 25.962 208 121 8 6 193 43 237 9.29e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045473.t1 AT3G17530 27.706 231 150 7 2 223 5 227 1.63e-12 68.2
MS.gene045473.t1 AT3G23880 25.862 232 133 8 2 215 13 223 2.56e-11 64.3
MS.gene045473.t1 AT1G11270 26.961 204 122 8 3 194 35 223 3.24e-11 62.8
MS.gene045473.t1 AT1G11270 25.822 213 134 7 3 203 35 235 5.82e-11 63.2
MS.gene045473.t1 AT1G11270 25.822 213 134 7 3 203 35 235 5.82e-11 63.2
MS.gene045473.t1 AT4G12560 29.744 195 113 9 6 189 7 188 7.88e-11 63.2
MS.gene045473.t1 AT4G12560 29.744 195 113 9 6 189 7 188 7.88e-11 63.2
MS.gene045473.t1 AT4G12560 29.744 195 113 9 6 189 7 188 7.88e-11 63.2

Find 65 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATGGTTTGGTTGGAAATA+TGG 0.186494 4.2:+29803039 MS.gene045473:CDS
AGATAAACATCAAGTAATTC+TGG 0.246226 4.2:-29802947 None:intergenic
TGTCTCAAAGGTATTTATTC+AGG 0.256979 4.2:+29802527 MS.gene045473:CDS
AATGAATTTAGGAGAAGATT+TGG 0.260801 4.2:+29802223 None:intergenic
TTTCATCATCTCATTGTTAT+CGG 0.302741 4.2:-29802708 None:intergenic
AGAGCAGATACAATGAATTT+AGG 0.315479 4.2:+29802212 None:intergenic
GTGAGGGAGCACTGGGAATA+AGG 0.322794 4.2:-29802604 None:intergenic
TTGATTCTTGCTACACCTTT+GGG 0.330484 4.2:-29802476 None:intergenic
TGATGACCTCATTGAGATAA+AGG 0.339238 4.2:+29802980 MS.gene045473:CDS
TTTGATTCTTGCTACACCTT+TGG 0.340798 4.2:-29802477 None:intergenic
GGAGTATAGGAATTCTAGTA+TGG 0.353777 4.2:+29803022 MS.gene045473:CDS
GTCACGATCAATTGATTGCT+TGG 0.387790 4.2:+29802558 MS.gene045473:CDS
AACAAAGATTTGATGAGGAT+TGG 0.402919 4.2:-29802452 None:intergenic
ACAAGAGTGTTGTATCTTGA+AGG 0.407653 4.2:-29802830 None:intergenic
AGATACAACACTCTTGTTAA+CGG 0.411459 4.2:+29802836 MS.gene045473:CDS
GGTGAACCTCATTAGTTGTT+GGG 0.430302 4.2:-29802583 None:intergenic
GTAGCAAGAATCAAAACTTA+TGG 0.431491 4.2:+29802485 MS.gene045473:CDS
ATAGGAATTCTAGTATGGTT+TGG 0.433927 4.2:+29803027 MS.gene045473:CDS
TCAAATGTGTTCAAAGATCA+TGG 0.438742 4.2:+29802291 MS.gene045473:CDS
ATTGAGATAAAGGGCTCTCT+TGG 0.445429 4.2:+29802990 MS.gene045473:CDS
GAATTCTAGTATGGTTTGGT+TGG 0.450780 4.2:+29803031 MS.gene045473:CDS
ACTTCTTCTGCTCACAAAGC+AGG 0.453122 4.2:-29802333 None:intergenic
ACATGAGAGAAGCTTGATGC+TGG 0.454156 4.2:-29802411 None:intergenic
GATAAACATCAAGTAATTCT+GGG 0.462331 4.2:-29802946 None:intergenic
ACACATTTGAATCTCATCAA+TGG 0.483025 4.2:-29802278 None:intergenic
TTGGTTATGTTGTGGAGTAT+AGG 0.485868 4.2:+29803009 MS.gene045473:CDS
GTTACGGAGTGAGGGAGCAC+TGG 0.488895 4.2:-29802612 None:intergenic
GGGCTCTCTTGGTTATGTTG+TGG 0.499919 4.2:+29803001 MS.gene045473:CDS
ATCTTGAAGGTTGTCGATGT+TGG 0.502139 4.2:-29802817 None:intergenic
TATTTATGGTATTTGGAATG+AGG 0.504084 4.2:+29803124 MS.gene045473:CDS
ATTTATCAATACAACCGTTA+CGG 0.504541 4.2:-29802628 None:intergenic
ATAATCTAAGCACTAAATCA+TGG 0.511976 4.2:+29802759 MS.gene045473:CDS
ATTTATGGTATTTGGAATGA+GGG 0.512971 4.2:+29803125 MS.gene045473:CDS
AGGTGAACCTCATTAGTTGT+TGG 0.516554 4.2:-29802584 None:intergenic
ATCAAATCTTTGTTTCCCAA+AGG 0.523197 4.2:+29802461 MS.gene045473:CDS
GATGACCTCATTGAGATAAA+GGG 0.525786 4.2:+29802981 MS.gene045473:CDS
CTTCTTCTGCTCACAAAGCA+GGG 0.543207 4.2:-29802332 None:intergenic
GCTGCTCAACATTTCCTCCA+AGG 0.547175 4.2:-29803157 None:intergenic
CTCACGTCATACGATCATCA+AGG 0.558496 4.2:+29803185 MS.gene045473:CDS
TGGGAAACAAAGATTTGATG+AGG 0.564142 4.2:-29802457 None:intergenic
AATACAACCGTTACGGAGTG+AGG 0.565225 4.2:-29802621 None:intergenic
TTGGAACCCAACAACTAATG+AGG 0.566976 4.2:+29802577 MS.gene045473:CDS
AATGAGGGTGCTGAAATCCT+TGG 0.569360 4.2:+29803140 MS.gene045473:CDS
TGTTCAATGATAAGAAGACT+AGG 0.576044 4.2:-29802371 None:intergenic
GAAAATGATTTATCTTACAT+CGG 0.578001 4.2:+29802727 MS.gene045473:CDS
CATGAGAGAAGCTTGATGCT+GGG 0.579942 4.2:-29802410 None:intergenic
TTGGTTGGAAATATGGATTG+TGG 0.581471 4.2:+29803046 MS.gene045473:CDS
GAGGGTGCTGAAATCCTTGG+AGG 0.581509 4.2:+29803143 MS.gene045473:CDS
AAAACTTATGGTAGTTGCAA+CGG 0.584601 4.2:+29802497 MS.gene045473:CDS
TTTGACAATAGAGCTATGCT+TGG 0.586263 4.2:-29803266 None:intergenic
ATCAAGCTTCTCTCATGTGA+CGG 0.587574 4.2:+29802416 MS.gene045473:CDS
GTTCAATGATAAGAAGACTA+GGG 0.591404 4.2:-29802370 None:intergenic
CGAGGAGGAAAATCGAGGAT+AGG 0.595302 4.2:-29802785 None:intergenic
AGTGCTCCCTCACTCCGTAA+CGG 0.599236 4.2:+29802614 MS.gene045473:CDS
TTACGGAGTGAGGGAGCACT+GGG 0.599398 4.2:-29802611 None:intergenic
ATACAACCGTTACGGAGTGA+GGG 0.632090 4.2:-29802620 None:intergenic
TGGAGACAGAGATCTTGTTG+CGG 0.647922 4.2:+29802243 MS.gene045473:CDS
TCACGTCATACGATCATCAA+GGG 0.648010 4.2:+29803186 MS.gene045473:CDS
ATAAACATCAAGTAATTCTG+GGG 0.648367 4.2:-29802945 None:intergenic
TTATCTCAATGAGGTCATCA+CGG 0.654243 4.2:-29802977 None:intergenic
TTGGAGGAAATGTTGAGCAG+CGG 0.664636 4.2:+29803159 MS.gene045473:CDS
GAGAGCCCTTTATCTCAATG+AGG 0.682373 4.2:-29802986 None:intergenic
CGATGTTGGACCATAATACG+AGG 0.683951 4.2:-29802803 None:intergenic
TAATACGAGGAGGAAAATCG+AGG 0.706310 4.2:-29802790 None:intergenic
TGTTGGACCATAATACGAGG+AGG 0.729728 4.2:-29802800 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAAATTTTAATTTGAAAT+CGG - chr4.2:29803223-29803242 None:intergenic 10.0%
!!! TGTATTGATAAATCATTTTA+TGG + chr4.2:29802638-29802657 MS.gene045473:CDS 15.0%
!! GAAAATGATTTATCTTACAT+CGG + chr4.2:29802727-29802746 MS.gene045473:CDS 20.0%
!!! AGTTTTGTATTTATGGTATT+TGG + chr4.2:29803117-29803136 MS.gene045473:CDS 20.0%
!!! GATAAATCATTTTATGGTTT+TGG + chr4.2:29802644-29802663 MS.gene045473:CDS 20.0%
! AGATAAACATCAAGTAATTC+TGG - chr4.2:29802950-29802969 None:intergenic 25.0%
! ATAAACATCAAGTAATTCTG+GGG - chr4.2:29802948-29802967 None:intergenic 25.0%
! ATAATCTAAGCACTAAATCA+TGG + chr4.2:29802759-29802778 MS.gene045473:CDS 25.0%
! ATTTATCAATACAACCGTTA+CGG - chr4.2:29802631-29802650 None:intergenic 25.0%
! GATAAACATCAAGTAATTCT+GGG - chr4.2:29802949-29802968 None:intergenic 25.0%
! TTTCATCATCTCATTGTTAT+CGG - chr4.2:29802711-29802730 None:intergenic 25.0%
!! ATATGGATTGTGGAAAAAAA+CGG + chr4.2:29803056-29803075 MS.gene045473:CDS 25.0%
!! ATTTATGGTATTTGGAATGA+GGG + chr4.2:29803125-29803144 MS.gene045473:CDS 25.0%
!! TATTTATGGTATTTGGAATG+AGG + chr4.2:29803124-29803143 MS.gene045473:CDS 25.0%
!!! CAGTCAAAGTTTTGTATTTA+TGG + chr4.2:29803110-29803129 MS.gene045473:CDS 25.0%
AAAACTTATGGTAGTTGCAA+CGG + chr4.2:29802497-29802516 MS.gene045473:CDS 30.0%
AACAAAGATTTGATGAGGAT+TGG - chr4.2:29802455-29802474 None:intergenic 30.0%
ACACATTTGAATCTCATCAA+TGG - chr4.2:29802281-29802300 None:intergenic 30.0%
AGATACAACACTCTTGTTAA+CGG + chr4.2:29802836-29802855 MS.gene045473:CDS 30.0%
ATAGGAATTCTAGTATGGTT+TGG + chr4.2:29803027-29803046 MS.gene045473:CDS 30.0%
GTAGCAAGAATCAAAACTTA+TGG + chr4.2:29802485-29802504 MS.gene045473:CDS 30.0%
GTTCAATGATAAGAAGACTA+GGG - chr4.2:29802373-29802392 None:intergenic 30.0%
TCAAATGTGTTCAAAGATCA+TGG + chr4.2:29802291-29802310 MS.gene045473:CDS 30.0%
TGTTCAATGATAAGAAGACT+AGG - chr4.2:29802374-29802393 None:intergenic 30.0%
TTGTTAACGGTGTTTATCAT+TGG + chr4.2:29802849-29802868 MS.gene045473:intron 30.0%
! ATCAAATCTTTGTTTCCCAA+AGG + chr4.2:29802461-29802480 MS.gene045473:CDS 30.0%
! TATGGATTGTGGAAAAAAAC+GGG + chr4.2:29803057-29803076 MS.gene045473:CDS 30.0%
!! TGTCTCAAAGGTATTTATTC+AGG + chr4.2:29802527-29802546 MS.gene045473:CDS 30.0%
GATGACCTCATTGAGATAAA+GGG + chr4.2:29802981-29803000 MS.gene045473:CDS 35.0%
GGAGTATAGGAATTCTAGTA+TGG + chr4.2:29803022-29803041 MS.gene045473:CDS 35.0%
TGATGACCTCATTGAGATAA+AGG + chr4.2:29802980-29802999 MS.gene045473:CDS 35.0%
TGGGAAACAAAGATTTGATG+AGG - chr4.2:29802460-29802479 None:intergenic 35.0%
TTATCTCAATGAGGTCATCA+CGG - chr4.2:29802980-29802999 None:intergenic 35.0%
TTGATTCTTGCTACACCTTT+GGG - chr4.2:29802479-29802498 None:intergenic 35.0%
TTGGTTATGTTGTGGAGTAT+AGG + chr4.2:29803009-29803028 MS.gene045473:CDS 35.0%
TTTGACAATAGAGCTATGCT+TGG - chr4.2:29803269-29803288 None:intergenic 35.0%
TTTGATTCTTGCTACACCTT+TGG - chr4.2:29802480-29802499 None:intergenic 35.0%
! TTGGTTGGAAATATGGATTG+TGG + chr4.2:29803046-29803065 MS.gene045473:CDS 35.0%
!! AATCTCATCAATGGTTTTGC+TGG - chr4.2:29802272-29802291 None:intergenic 35.0%
!! ACAAGAGTGTTGTATCTTGA+AGG - chr4.2:29802833-29802852 None:intergenic 35.0%
!! GAATTCTAGTATGGTTTGGT+TGG + chr4.2:29803031-29803050 MS.gene045473:CDS 35.0%
!! GTATGGTTTGGTTGGAAATA+TGG + chr4.2:29803039-29803058 MS.gene045473:CDS 35.0%
!!! AACGGTGTTTTTTGTCTCAA+AGG + chr4.2:29802515-29802534 MS.gene045473:CDS 35.0%
!!! TATGGTTTTGGTGCTGTTAA+TGG + chr4.2:29802656-29802675 MS.gene045473:CDS 35.0%
AGGTGAACCTCATTAGTTGT+TGG - chr4.2:29802587-29802606 None:intergenic 40.0%
ATCAAGCTTCTCTCATGTGA+CGG + chr4.2:29802416-29802435 MS.gene045473:CDS 40.0%
ATCTTGAAGGTTGTCGATGT+TGG - chr4.2:29802820-29802839 None:intergenic 40.0%
ATTGAGATAAAGGGCTCTCT+TGG + chr4.2:29802990-29803009 MS.gene045473:CDS 40.0%
GATTGTGGAAAAAAACGGGT+GGG + chr4.2:29803061-29803080 MS.gene045473:CDS 40.0%
GGTGAACCTCATTAGTTGTT+GGG - chr4.2:29802586-29802605 None:intergenic 40.0%
GTCACGATCAATTGATTGCT+TGG + chr4.2:29802558-29802577 MS.gene045473:CDS 40.0%
TAATACGAGGAGGAAAATCG+AGG - chr4.2:29802793-29802812 None:intergenic 40.0%
TCACGTCATACGATCATCAA+GGG + chr4.2:29803186-29803205 MS.gene045473:CDS 40.0%
TTGGAACCCAACAACTAATG+AGG + chr4.2:29802577-29802596 MS.gene045473:CDS 40.0%
! CGATTTTCCTCCTCGTATTA+TGG + chr4.2:29802793-29802812 MS.gene045473:CDS 40.0%
! GATGCTGGGTCTTTTCAATT+TGG - chr4.2:29802399-29802418 None:intergenic 40.0%
AATACAACCGTTACGGAGTG+AGG - chr4.2:29802624-29802643 None:intergenic 45.0%
ACATGAGAGAAGCTTGATGC+TGG - chr4.2:29802414-29802433 None:intergenic 45.0%
ACTTCTTCTGCTCACAAAGC+AGG - chr4.2:29802336-29802355 None:intergenic 45.0%
ATACAACCGTTACGGAGTGA+GGG - chr4.2:29802623-29802642 None:intergenic 45.0%
CATGAGAGAAGCTTGATGCT+GGG - chr4.2:29802413-29802432 None:intergenic 45.0%
CGATGTTGGACCATAATACG+AGG - chr4.2:29802806-29802825 None:intergenic 45.0%
CTCACGTCATACGATCATCA+AGG + chr4.2:29803185-29803204 MS.gene045473:CDS 45.0%
CTTCTTCTGCTCACAAAGCA+GGG - chr4.2:29802335-29802354 None:intergenic 45.0%
GAGAGCCCTTTATCTCAATG+AGG - chr4.2:29802989-29803008 None:intergenic 45.0%
GGATTGTGGAAAAAAACGGG+TGG + chr4.2:29803060-29803079 MS.gene045473:CDS 45.0%
TGGAGACAGAGATCTTGTTG+CGG + chr4.2:29802243-29802262 MS.gene045473:CDS 45.0%
TGTTGGACCATAATACGAGG+AGG - chr4.2:29802803-29802822 None:intergenic 45.0%
TTGGAGGAAATGTTGAGCAG+CGG + chr4.2:29803159-29803178 MS.gene045473:CDS 45.0%
!! AATGAGGGTGCTGAAATCCT+TGG + chr4.2:29803140-29803159 MS.gene045473:CDS 45.0%
!!! AAATTAAAATTTCAAATTTT+AGG + chr4.2:29803228-29803247 MS.gene045473:CDS 5.0%
CGAGGAGGAAAATCGAGGAT+AGG - chr4.2:29802788-29802807 None:intergenic 50.0%
GCTGCTCAACATTTCCTCCA+AGG - chr4.2:29803160-29803179 None:intergenic 50.0%
GGGCTCTCTTGGTTATGTTG+TGG + chr4.2:29803001-29803020 MS.gene045473:CDS 50.0%
AGTGCTCCCTCACTCCGTAA+CGG + chr4.2:29802614-29802633 MS.gene045473:CDS 55.0%
!! GAGGGTGCTGAAATCCTTGG+AGG + chr4.2:29803143-29803162 MS.gene045473:CDS 55.0%
!! GTGAGGGAGCACTGGGAATA+AGG - chr4.2:29802607-29802626 None:intergenic 55.0%
!! TTACGGAGTGAGGGAGCACT+GGG - chr4.2:29802614-29802633 None:intergenic 55.0%
! GTTACGGAGTGAGGGAGCAC+TGG - chr4.2:29802615-29802634 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 29802224 29803292 29802224 ID=MS.gene045473
chr4.2 mRNA 29802224 29803292 29802224 ID=MS.gene045473.t1;Parent=MS.gene045473
chr4.2 exon 29802224 29802859 29802224 ID=MS.gene045473.t1.exon1;Parent=MS.gene045473.t1
chr4.2 CDS 29802224 29802859 29802224 ID=cds.MS.gene045473.t1;Parent=MS.gene045473.t1
chr4.2 exon 29802945 29803292 29802945 ID=MS.gene045473.t1.exon2;Parent=MS.gene045473.t1
chr4.2 CDS 29802945 29803292 29802945 ID=cds.MS.gene045473.t1;Parent=MS.gene045473.t1
Gene Sequence

>MS.gene045473

ATGAATTTAGGAGAAGATTTGGAGACAGAGATCTTGTTGCGGCTTCCAGCAAAACCATTGATGAGATTCAAATGTGTTCAAAGATCATGGAACAGTCTCATCAAAACTCCCTGCTTTGTGAGCAGAAGAAGTAAGTTGCAACGAAACCCTAGTCTTCTTATCATTGAACAGTCCAAATTGAAAAGACCCAGCATCAAGCTTCTCTCATGTGACGGTGACAACGAGAAACCAATCCTCATCAAATCTTTGTTTCCCAAAGGTGTAGCAAGAATCAAAACTTATGGTAGTTGCAACGGTGTTTTTTGTCTCAAAGGTATTTATTCAGGCAAAACTTGTCACGATCAATTGATTGCTTGGAACCCAACAACTAATGAGGTTCACCTTATTCCCAGTGCTCCCTCACTCCGTAACGGTTGTATTGATAAATCATTTTATGGTTTTGGTGCTGTTAATGGCGACTTCAAACTTGTCAAACTCAATATCTCCGATAACAATGAGATGATGAAAATGATTTATCTTACATCGGCAGAAGTTTATAATCTAAGCACTAAATCATGGACACCTATCCTCGATTTTCCTCCTCGTATTATGGTCCAACATCGACAACCTTCAAGATACAACACTCTTGTTAACGGTGTTTATCATTGGATTACGAGCTCTTACTATACTACAAAAATTCTTTGCTTTGACTTTCACAATAACAAGTTTCAGCAACTTGAAGCCCCAGAATTACTTGATGTTTATCTCATATTTCCGTGATGACCTCATTGAGATAAAGGGCTCTCTTGGTTATGTTGTGGAGTATAGGAATTCTAGTATGGTTTGGTTGGAAATATGGATTGTGGAAAAAAACGGGTGGGACAAAAAGTATAACTTTGACACAACTCAGTCAAAGTTTTGTATTTATGGTATTTGGAATGAGGGTGCTGAAATCCTTGGAGGAAATGTTGAGCAGCGGAAGCTCACGTCATACGATCATCAAGGGAACGCACTTCGCCGATTTCAAATTAAAATTTCAAATTTTAGGATTTATGAGTATGTGCCAAGCATAGCTCTATTGTCAAAGTAG
Protein sequence

>MS.gene045473.t1

MNLGEDLETEILLRLPAKPLMRFKCVQRSWNSLIKTPCFVSRRSKLQRNPSLLIIEQSKLKRPSIKLLSCDGDNEKPILIKSLFPKGVARIKTYGSCNGVFCLKGIYSGKTCHDQLIAWNPTTNEVHLIPSAPSLRNGCIDKSFYGFGAVNGDFKLVKLNISDNNEMMKMIYLTSAEVYNLSTKSWTPILDFPPRIMVQHRQPSRYNTLVNGPQNYLMFISYFRDDLIEIKGSLGYVVEYRNSSMVWLEIWIVEKNGWDKKYNFDTTQSKFCIYGIWNEGAEILGGNVEQRKLTSYDHQGNALRRFQIKISNFRIYEYVPSIALLSK