AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51191


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51191.t1 MTR_8g102520 75.287 174 43 0 3 176 149 322 2.98e-92 271
MS.gene51191.t1 MTR_7g079450 78.571 140 26 1 37 176 2 137 1.29e-76 225
MS.gene51191.t1 MTR_7g079400 63.128 179 62 1 2 176 159 337 1.19e-73 224
MS.gene51191.t1 MTR_8g098830 72.297 148 40 1 3 150 54 200 2.00e-73 220
MS.gene51191.t1 MTR_7g079370 58.721 172 70 1 2 173 183 353 4.35e-59 188
MS.gene51191.t1 MTR_7g079420 59.551 178 60 6 2 172 968 1140 3.34e-58 196
MS.gene51191.t1 MTR_2g048580 54.444 180 64 6 2 176 176 342 6.02e-53 172
MS.gene51191.t1 MTR_6g060650 48.571 175 82 4 3 175 204 372 9.88e-43 146
MS.gene51191.t1 MTR_4g117720 45.506 178 85 5 2 172 201 373 8.05e-42 144
MS.gene51191.t1 MTR_4g109280 48.555 173 77 7 2 169 187 352 3.45e-40 139
MS.gene51191.t1 MTR_4g116320 48.734 158 76 3 16 170 97 252 6.50e-38 130
MS.gene51191.t1 MTR_1g055175 41.520 171 96 3 2 170 27 195 7.09e-38 129
MS.gene51191.t1 MTR_5g070088 47.500 160 78 4 17 172 224 381 7.80e-38 134
MS.gene51191.t1 MTR_5g070080 48.447 161 75 5 16 172 225 381 1.26e-35 128
MS.gene51191.t1 MTR_1g055195 42.236 161 92 1 15 175 318 477 1.88e-35 129
MS.gene51191.t1 MTR_4g094365 47.500 160 76 5 16 170 202 358 3.43e-35 126
MS.gene51191.t1 MTR_5g070130 46.497 157 78 4 20 172 218 372 4.23e-35 126
MS.gene51191.t1 MTR_4g116330 48.428 159 75 4 17 170 218 374 5.40e-35 126
MS.gene51191.t1 MTR_1g055165 42.073 164 91 3 9 170 151 312 1.67e-34 123
MS.gene51191.t1 MTR_5g097210 43.931 173 88 6 2 171 192 358 2.31e-34 124
MS.gene51191.t1 MTR_4g122850 45.509 167 84 6 8 170 195 358 4.47e-34 124
MS.gene51191.t1 MTR_3g103020 42.775 173 89 5 2 170 206 372 9.84e-34 122
MS.gene51191.t1 MTR_4g118380 47.518 141 65 2 17 148 217 357 1.38e-33 122
MS.gene51191.t1 MTR_4g118400 42.105 171 79 5 2 170 142 294 1.63e-33 120
MS.gene51191.t1 MTR_4g023080 45.570 158 76 4 16 169 196 347 1.80e-33 121
MS.gene51191.t1 MTR_4g023130 44.828 174 78 6 2 169 145 306 4.09e-33 120
MS.gene51191.t1 MTR_4g118360 50.000 120 59 1 52 170 1 120 9.59e-33 114
MS.gene51191.t1 MTR_1g055135 39.888 178 104 2 2 176 198 375 1.70e-32 119
MS.gene51191.t1 MTR_8g040935 40.909 176 93 6 2 170 31 202 1.91e-32 115
MS.gene51191.t1 MTR_4g109270 41.379 174 93 5 2 170 189 358 4.85e-32 118
MS.gene51191.t1 MTR_4g117750 46.707 167 81 3 15 175 194 358 9.38e-32 119
MS.gene51191.t1 MTR_4g117750 42.105 133 66 5 48 170 346 477 8.59e-16 74.3
MS.gene51191.t1 MTR_8g433030 46.250 160 76 6 18 172 184 338 2.50e-31 115
MS.gene51191.t1 MTR_8g032390 46.250 160 76 6 18 172 217 371 4.30e-31 115
MS.gene51191.t1 MTR_4g021785 42.286 175 85 6 1 169 184 348 4.85e-31 115
MS.gene51191.t1 MTR_3g034180 45.062 162 75 5 15 170 330 483 2.80e-30 115
MS.gene51191.t1 MTR_4g023190 42.941 170 88 4 7 170 187 353 5.15e-30 112
MS.gene51191.t1 MTR_4g118370 42.038 157 71 3 15 171 119 255 6.70e-29 107
MS.gene51191.t1 MTR_6g463430 41.860 172 89 6 2 170 33 196 9.36e-28 103
MS.gene51191.t1 MTR_4g023200 42.236 161 84 4 16 170 197 354 1.51e-27 106
MS.gene51191.t1 MTR_4g023090 39.306 173 82 6 16 170 211 378 3.33e-27 105
MS.gene51191.t1 MTR_4g023070 39.645 169 80 5 18 169 200 363 3.57e-27 105
MS.gene51191.t1 MTR_3g034160 40.223 179 93 5 2 175 205 374 3.62e-27 105
MS.gene51191.t1 MTR_4g117850 39.759 166 91 4 14 172 213 376 3.63e-27 105
MS.gene51191.t1 MTR_3g034130 41.549 142 69 3 15 149 216 350 5.99e-27 104
MS.gene51191.t1 MTR_8g433040 44.304 158 70 5 16 170 216 358 6.09e-27 104
MS.gene51191.t1 MTR_8g032380 44.304 158 70 5 16 170 216 358 6.09e-27 104
MS.gene51191.t1 MTR_4g023150 42.138 159 83 4 18 170 199 354 7.51e-27 104
MS.gene51191.t1 MTR_4g117770 44.099 161 84 4 15 170 202 361 2.44e-25 100
MS.gene51191.t1 MTR_4g117810 44.099 161 84 4 15 170 202 361 2.44e-25 100
MS.gene51191.t1 MTR_4g023120 39.645 169 80 6 18 169 200 363 7.20e-25 99.0
MS.gene51191.t1 MTR_4g023380 37.569 181 82 5 16 170 196 371 8.89e-25 99.0
MS.gene51191.t1 MTR_4g117780 41.279 172 93 5 4 170 193 361 1.33e-24 98.6
MS.gene51191.t1 MTR_1g055250 40.441 136 76 4 15 149 110 241 1.01e-23 95.5
MS.gene51191.t1 MTR_4g117870 45.385 130 66 4 49 176 141 267 1.12e-23 94.4
MS.gene51191.t1 MTR_4g117530 35.673 171 71 5 2 170 114 247 1.17e-23 94.0
MS.gene51191.t1 MTR_5g023000 52.874 87 38 2 86 170 8 93 5.82e-21 82.8
MS.gene51191.t1 MTR_4g116330 49.091 110 51 3 66 170 226 335 4.77e-20 85.9
MS.gene51191.t1 MTR_4g023440 33.918 171 67 4 2 170 200 326 1.47e-18 81.6
MS.gene51191.t1 MTR_7g117720 50.000 98 40 4 81 171 177 272 2.37e-18 80.1
MS.gene51191.t1 MTR_4g122860 39.850 133 62 6 53 175 195 319 2.44e-17 78.2
MS.gene51191.t1 MTR_4g114700 42.857 105 53 3 70 167 114 218 5.68e-13 65.1
MS.gene51191.t1 MTR_4g117430 43.421 76 39 2 2 77 80 151 3.64e-12 61.6
MS.gene51191.t1 MTR_6g021880 30.588 170 111 5 3 168 215 381 8.36e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATTGCCTTTGATTTAA+AGG 0.116438 7.3:-27693304 MS.gene51191:CDS
ATTTATGATTTGATGGTATT+TGG 0.157846 7.3:-27693222 MS.gene51191:CDS
TCTTGAATGGTTCTATTTAT+TGG 0.189301 7.3:-27693359 MS.gene51191:CDS
CTCTGTATACACCGCCATTT+TGG 0.207122 7.3:+27692948 None:intergenic
CACGATTTGTAAAGCATATT+GGG 0.232410 7.3:+27693073 None:intergenic
CTGGTATGTACTTGCCTTAT+AGG 0.235793 7.3:-27693422 None:intergenic
CCACGATTTGTAAAGCATAT+TGG 0.239313 7.3:+27693072 None:intergenic
GGCAATAATAACATCGCTTT+TGG 0.269149 7.3:+27693317 None:intergenic
CCGACTCTGGGACCACCTTC+AGG 0.271870 7.3:+27693387 None:intergenic
TCTTAGAATTTATGATTTGA+TGG 0.287400 7.3:-27693229 MS.gene51191:CDS
CTTTCACTCCCCGATGGTAT+TGG 0.294504 7.3:-27692919 MS.gene51191:CDS
GGGCTTGCAATATTCCTATA+AGG 0.347352 7.3:+27693408 None:intergenic
TTACTTTATGAGATCCAAAA+TGG 0.350207 7.3:-27692962 MS.gene51191:CDS
GAATGGTTCTATTTATTGGC+TGG 0.370215 7.3:-27693355 MS.gene51191:CDS
TTTCACTCCCCGATGGTATT+GGG 0.375560 7.3:-27692918 MS.gene51191:CDS
AGAGTTCTAAAATTAAGATA+TGG 0.380976 7.3:-27693173 MS.gene51191:CDS
TTGGCGGACTTATCAGTGTA+TGG 0.416802 7.3:-27693203 MS.gene51191:CDS
TGACCACTATTAAAATCATC+TGG 0.425662 7.3:+27693255 None:intergenic
CTGAAGGTGGTCCCAGAGTC+GGG 0.433638 7.3:-27693386 MS.gene51191:CDS
CGACTCTGGGACCACCTTCA+GGG 0.442516 7.3:+27693388 None:intergenic
ACAAATCGTGGTGATATAGT+TGG 0.444100 7.3:-27693060 MS.gene51191:CDS
ATAGTTGGTCCAGTTGTTGA+TGG 0.466569 7.3:-27693045 MS.gene51191:CDS
TGTCATTTCCTTTAAATCAA+AGG 0.469429 7.3:+27693296 None:intergenic
ATTCAAGAATGACCCGACTC+TGG 0.470304 7.3:+27693374 None:intergenic
GCTAATCCACCATCAACAAC+TGG 0.481967 7.3:+27693036 None:intergenic
AATATGCAGTGCATTCATCT+TGG 0.489224 7.3:-27693140 MS.gene51191:CDS
GAGTTCTAAAATTAAGATAT+GGG 0.492351 7.3:-27693172 MS.gene51191:CDS
TAAGTTTGCCCAATACCATC+GGG 0.492784 7.3:+27692910 None:intergenic
TATGATTTGATGGTATTTGG+CGG 0.504914 7.3:-27693219 MS.gene51191:CDS
AGGAATATTGCAAGCCCTGA+AGG 0.512532 7.3:-27693402 MS.gene51191:CDS
TTAAGTTTGCCCAATACCAT+CGG 0.522120 7.3:+27692909 None:intergenic
CTGCAAGAGTATCATTCCTA+TGG 0.523562 7.3:-27692991 MS.gene51191:CDS
GTTGGTCCAGTTGTTGATGG+TGG 0.526964 7.3:-27693042 MS.gene51191:CDS
CCTGAAGGTGGTCCCAGAGT+CGG 0.527660 7.3:-27693387 MS.gene51191:CDS
CCAATATGCTTTACAAATCG+TGG 0.535731 7.3:-27693072 MS.gene51191:CDS
AGAGTCGGGTCATTCTTGAA+TGG 0.540114 7.3:-27693372 MS.gene51191:CDS
TTCAAGAATGACCCGACTCT+GGG 0.541376 7.3:+27693375 None:intergenic
CATAAAGTAACGGTCACCAT+AGG 0.590340 7.3:+27692975 None:intergenic
ACGATTTGTAAAGCATATTG+GGG 0.609876 7.3:+27693074 None:intergenic
TCTCTGCTTTCACTCCCCGA+TGG 0.651085 7.3:-27692925 MS.gene51191:CDS
TTAGCGAAGCTTAATGACAA+AGG 0.665656 7.3:-27693018 MS.gene51191:CDS
AATATTGCAAGCCCTGAAGG+TGG 0.669896 7.3:-27693399 MS.gene51191:CDS
CTTTATGAGATCCAAAATGG+CGG 0.689205 7.3:-27692959 MS.gene51191:CDS
AAGTTTGCCCAATACCATCG+GGG 0.746553 7.3:+27692911 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTAGAATTTATGATTTGA+TGG - chr7.3:27693097-27693116 MS.gene51191:CDS 20.0%
!!! AGAGTTCTAAAATTAAGATA+TGG - chr7.3:27693153-27693172 MS.gene51191:CDS 20.0%
!!! ATTTATGATTTGATGGTATT+TGG - chr7.3:27693104-27693123 MS.gene51191:CDS 20.0%
!!! GAGTTCTAAAATTAAGATAT+GGG - chr7.3:27693154-27693173 MS.gene51191:CDS 20.0%
!!! TATTATTGCCTTTGATTTAA+AGG - chr7.3:27693022-27693041 MS.gene51191:CDS 20.0%
! TTACTTTATGAGATCCAAAA+TGG - chr7.3:27693364-27693383 MS.gene51191:CDS 25.0%
!! TCTTGAATGGTTCTATTTAT+TGG - chr7.3:27692967-27692986 MS.gene51191:CDS 25.0%
!! TGTCATTTCCTTTAAATCAA+AGG + chr7.3:27693033-27693052 None:intergenic 25.0%
!! TTTTGGATCTCATAAAGTAA+CGG + chr7.3:27693364-27693383 None:intergenic 25.0%
ACGATTTGTAAAGCATATTG+GGG + chr7.3:27693255-27693274 None:intergenic 30.0%
CACGATTTGTAAAGCATATT+GGG + chr7.3:27693256-27693275 None:intergenic 30.0%
TGACCACTATTAAAATCATC+TGG + chr7.3:27693074-27693093 None:intergenic 30.0%
! TATGATTTGATGGTATTTGG+CGG - chr7.3:27693107-27693126 MS.gene51191:CDS 30.0%
!! CTTCCAGATGATTTTAATAG+TGG - chr7.3:27693068-27693087 MS.gene51191:CDS 30.0%
AAGCATATTGGGGAAAAATC+CGG + chr7.3:27693245-27693264 None:intergenic 35.0%
AATATGCAGTGCATTCATCT+TGG - chr7.3:27693186-27693205 MS.gene51191:CDS 35.0%
ACAAATCGTGGTGATATAGT+TGG - chr7.3:27693266-27693285 MS.gene51191:CDS 35.0%
CCAATATGCTTTACAAATCG+TGG - chr7.3:27693254-27693273 MS.gene51191:CDS 35.0%
CCACGATTTGTAAAGCATAT+TGG + chr7.3:27693257-27693276 None:intergenic 35.0%
CTTTATGAGATCCAAAATGG+CGG - chr7.3:27693367-27693386 MS.gene51191:CDS 35.0%
TTAAGTTTGCCCAATACCAT+CGG + chr7.3:27693420-27693439 None:intergenic 35.0%
TTAGCGAAGCTTAATGACAA+AGG - chr7.3:27693308-27693327 MS.gene51191:CDS 35.0%
! GAATGGTTCTATTTATTGGC+TGG - chr7.3:27692971-27692990 MS.gene51191:CDS 35.0%
! GGCAATAATAACATCGCTTT+TGG + chr7.3:27693012-27693031 None:intergenic 35.0%
ATAGTTGGTCCAGTTGTTGA+TGG - chr7.3:27693281-27693300 MS.gene51191:CDS 40.0%
CATAAAGTAACGGTCACCAT+AGG + chr7.3:27693354-27693373 None:intergenic 40.0%
GGGCTTGCAATATTCCTATA+AGG + chr7.3:27692921-27692940 None:intergenic 40.0%
TAAGTTTGCCCAATACCATC+GGG + chr7.3:27693419-27693438 None:intergenic 40.0%
! TTTTTCTTTTCATCCCGCTC+CGG - chr7.3:27693223-27693242 MS.gene51191:CDS 40.0%
!! CTGCAAGAGTATCATTCCTA+TGG - chr7.3:27693335-27693354 MS.gene51191:CDS 40.0%
AAGTTTGCCCAATACCATCG+GGG + chr7.3:27693418-27693437 None:intergenic 45.0%
AATATTGCAAGCCCTGAAGG+TGG - chr7.3:27692927-27692946 MS.gene51191:CDS 45.0%
AGAGTCGGGTCATTCTTGAA+TGG - chr7.3:27692954-27692973 MS.gene51191:CDS 45.0%
AGGAATATTGCAAGCCCTGA+AGG - chr7.3:27692924-27692943 MS.gene51191:CDS 45.0%
ATTCAAGAATGACCCGACTC+TGG + chr7.3:27692955-27692974 None:intergenic 45.0%
GCTAATCCACCATCAACAAC+TGG + chr7.3:27693293-27693312 None:intergenic 45.0%
TATTGGGGAAAAATCCGGAG+CGG + chr7.3:27693240-27693259 None:intergenic 45.0%
TTCAAGAATGACCCGACTCT+GGG + chr7.3:27692954-27692973 None:intergenic 45.0%
TTTCACTCCCCGATGGTATT+GGG - chr7.3:27693408-27693427 MS.gene51191:CDS 45.0%
! CTCTGTATACACCGCCATTT+TGG + chr7.3:27693381-27693400 None:intergenic 45.0%
!! TTGGCGGACTTATCAGTGTA+TGG - chr7.3:27693123-27693142 MS.gene51191:CDS 45.0%
ATTGGGGAAAAATCCGGAGC+GGG + chr7.3:27693239-27693258 None:intergenic 50.0%
CTTTCACTCCCCGATGGTAT+TGG - chr7.3:27693407-27693426 MS.gene51191:CDS 50.0%
! GTTGGTCCAGTTGTTGATGG+TGG - chr7.3:27693284-27693303 MS.gene51191:CDS 50.0%
TCTCTGCTTTCACTCCCCGA+TGG - chr7.3:27693401-27693420 MS.gene51191:CDS 55.0%
CCTGAAGGTGGTCCCAGAGT+CGG - chr7.3:27692939-27692958 MS.gene51191:CDS 60.0%
CTGAAGGTGGTCCCAGAGTC+GGG - chr7.3:27692940-27692959 MS.gene51191:CDS 60.0%
!! CGACTCTGGGACCACCTTCA+GGG + chr7.3:27692941-27692960 None:intergenic 60.0%
!! CCGACTCTGGGACCACCTTC+AGG + chr7.3:27692942-27692961 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 27692909 27693439 27692909 ID=MS.gene51191
chr7.3 mRNA 27692909 27693439 27692909 ID=MS.gene51191.t1;Parent=MS.gene51191
chr7.3 exon 27692909 27693439 27692909 ID=MS.gene51191.t1.exon1;Parent=MS.gene51191.t1
chr7.3 CDS 27692909 27693439 27692909 ID=cds.MS.gene51191.t1;Parent=MS.gene51191.t1
Gene Sequence

>MS.gene51191

ATGTACTTGCCTTATAGGAATATTGCAAGCCCTGAAGGTGGTCCCAGAGTCGGGTCATTCTTGAATGGTTCTATTTATTGGCTGGTTTATAATTATGAGACCAAAAGCGATGTTATTATTGCCTTTGATTTAAAGGAAATGACACTGTCAGAGATAGCTCTTCCAGATGATTTTAATAGTGGTCACTCTCTTAGAATTTATGATTTGATGGTATTTGGCGGACTTATCAGTGTATGGATTGTGAAGAGTTCTAAAATTAAGATATGGGTGATGCAAGAATATGCAGTGCATTCATCTTGGACTAAGACTCTTGATTTTTCTTTTCATCCCGCTCCGGATTTTTCCCCAATATGCTTTACAAATCGTGGTGATATAGTTGGTCCAGTTGTTGATGGTGGATTAGCGAAGCTTAATGACAAAGGACAACTGCAAGAGTATCATTCCTATGGTGACCGTTACTTTATGAGATCCAAAATGGCGGTGTATACAGAGTCTCTGCTTTCACTCCCCGATGGTATTGGGCAAACTTAA

Protein sequence

>MS.gene51191.t1

MYLPYRNIASPEGGPRVGSFLNGSIYWLVYNYETKSDVIIAFDLKEMTLSEIALPDDFNSGHSLRIYDLMVFGGLISVWIVKSSKIKIWVMQEYAVHSSWTKTLDFSFHPAPDFSPICFTNRGDIVGPVVDGGLAKLNDKGQLQEYHSYGDRYFMRSKMAVYTESLLSLPDGIGQT