AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50297


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50297.t1 MTR_4g023380 66.216 148 26 3 1 124 232 379 4.39e-58 184
MS.gene50297.t1 MTR_4g023090 63.571 140 35 3 1 124 247 386 7.16e-54 173
MS.gene50297.t1 MTR_4g023130 70.968 124 33 2 1 123 191 312 6.63e-51 164
MS.gene50297.t1 MTR_4g023080 71.774 124 32 2 1 123 232 353 2.53e-50 163
MS.gene50297.t1 MTR_4g023070 62.500 136 38 2 1 123 234 369 3.46e-50 163
MS.gene50297.t1 MTR_4g023120 61.314 137 40 2 1 124 234 370 1.11e-48 159
MS.gene50297.t1 MTR_4g021785 68.548 124 36 2 1 123 233 354 2.37e-48 158
MS.gene50297.t1 MTR_4g109270 63.115 122 42 1 6 124 243 364 5.41e-46 152
MS.gene50297.t1 MTR_4g023440 67.241 116 28 2 10 124 226 332 4.03e-45 149
MS.gene50297.t1 MTR_3g103020 63.200 125 42 3 3 124 255 378 9.07e-45 149
MS.gene50297.t1 MTR_4g116330 61.240 129 44 3 1 124 253 380 1.49e-44 149
MS.gene50297.t1 MTR_8g040935 58.915 129 47 2 1 124 81 208 1.93e-44 144
MS.gene50297.t1 MTR_5g023000 65.657 99 31 1 29 124 1 99 2.98e-41 132
MS.gene50297.t1 MTR_4g117870 56.693 127 49 4 1 124 144 267 5.34e-41 137
MS.gene50297.t1 MTR_4g117780 65.179 112 36 2 16 124 256 367 8.21e-41 139
MS.gene50297.t1 MTR_4g116320 61.905 126 40 4 1 121 133 255 9.78e-41 136
MS.gene50297.t1 MTR_4g117770 65.179 112 36 2 16 124 256 367 1.03e-40 139
MS.gene50297.t1 MTR_4g117810 65.179 112 36 2 16 124 256 367 1.03e-40 139
MS.gene50297.t1 MTR_6g060650 55.556 126 50 3 1 123 250 372 4.00e-40 137
MS.gene50297.t1 MTR_4g094365 59.055 127 47 4 1 124 240 364 4.38e-40 137
MS.gene50297.t1 MTR_4g023190 64.865 111 36 3 15 122 247 357 1.12e-39 135
MS.gene50297.t1 MTR_8g433040 58.537 123 48 3 3 124 244 364 3.71e-39 134
MS.gene50297.t1 MTR_8g032380 58.537 123 48 3 3 124 244 364 3.71e-39 134
MS.gene50297.t1 MTR_4g118360 61.207 116 36 4 13 123 14 125 1.34e-38 126
MS.gene50297.t1 MTR_4g023150 63.964 111 37 3 15 122 248 358 1.58e-38 132
MS.gene50297.t1 MTR_4g023200 63.063 111 38 3 15 122 248 358 6.40e-38 131
MS.gene50297.t1 MTR_3g034180 60.630 127 43 5 1 124 367 489 6.91e-38 133
MS.gene50297.t1 MTR_8g433030 58.678 121 46 4 1 119 219 337 2.82e-37 129
MS.gene50297.t1 MTR_8g032390 58.678 121 46 4 1 119 252 370 7.18e-37 129
MS.gene50297.t1 MTR_4g116330 61.062 113 39 2 17 124 229 341 7.80e-37 128
MS.gene50297.t1 MTR_4g109280 57.759 116 41 4 14 124 247 359 2.78e-36 127
MS.gene50297.t1 MTR_4g117530 54.310 116 51 1 9 124 140 253 1.28e-35 122
MS.gene50297.t1 MTR_6g463430 52.756 127 52 2 1 124 81 202 5.45e-35 119
MS.gene50297.t1 MTR_4g117720 51.880 133 52 4 1 124 249 378 7.65e-35 123
MS.gene50297.t1 MTR_4g122850 55.039 129 50 6 1 124 239 364 1.59e-34 122
MS.gene50297.t1 MTR_5g070130 53.906 128 55 2 1 124 250 377 1.60e-34 123
MS.gene50297.t1 MTR_4g117850 56.000 125 49 4 1 121 254 376 3.13e-34 122
MS.gene50297.t1 MTR_3g034160 65.138 109 34 3 17 123 268 374 8.62e-34 120
MS.gene50297.t1 MTR_4g117750 55.172 116 47 2 11 122 367 481 2.02e-32 119
MS.gene50297.t1 MTR_4g117750 46.721 122 58 3 5 123 241 358 1.30e-26 102
MS.gene50297.t1 MTR_1g055195 52.000 125 52 4 1 121 355 475 6.47e-32 117
MS.gene50297.t1 MTR_5g097210 53.279 122 48 5 3 120 243 359 2.49e-31 114
MS.gene50297.t1 MTR_5g070080 58.621 116 43 3 13 124 272 386 4.25e-31 114
MS.gene50297.t1 MTR_3g034130 58.095 105 40 3 1 104 253 354 2.29e-30 111
MS.gene50297.t1 MTR_1g055175 48.438 128 57 5 1 123 77 200 3.35e-30 107
MS.gene50297.t1 MTR_4g118370 48.387 124 56 5 1 121 139 257 4.01e-30 108
MS.gene50297.t1 MTR_4g118380 57.143 98 39 2 3 100 263 357 5.69e-30 110
MS.gene50297.t1 MTR_4g118400 59.551 89 33 1 33 118 206 294 2.91e-29 107
MS.gene50297.t1 MTR_1g055165 48.438 128 57 5 1 123 194 317 9.45e-29 106
MS.gene50297.t1 MTR_4g122860 52.174 115 45 3 17 124 209 320 3.98e-28 105
MS.gene50297.t1 MTR_5g070088 49.219 128 61 2 1 124 259 386 4.32e-28 105
MS.gene50297.t1 MTR_4g023430 65.854 82 22 2 49 124 141 222 8.82e-28 102
MS.gene50297.t1 MTR_1g055135 44.615 130 62 4 1 123 248 374 1.21e-26 102
MS.gene50297.t1 MTR_7g117720 63.218 87 25 2 40 119 186 272 1.39e-26 100
MS.gene50297.t1 MTR_1g055250 48.246 114 55 3 1 114 146 255 1.78e-26 100
MS.gene50297.t1 MTR_7g079450 47.748 111 50 3 17 123 30 136 1.99e-23 88.6
MS.gene50297.t1 MTR_8g102520 47.321 112 51 3 17 124 215 322 4.69e-23 91.7
MS.gene50297.t1 MTR_4g023100 80.000 55 10 1 71 124 65 119 2.45e-22 85.1
MS.gene50297.t1 MTR_4g114700 53.261 92 37 1 30 115 127 218 5.17e-22 87.4
MS.gene50297.t1 MTR_4g121580 47.826 115 50 3 1 114 234 339 5.89e-22 89.4
MS.gene50297.t1 MTR_7g079370 49.057 106 46 3 17 118 249 350 1.04e-21 88.6
MS.gene50297.t1 MTR_7g079400 46.552 116 50 4 17 124 226 337 2.36e-21 87.4
MS.gene50297.t1 MTR_8g098830 50.000 84 37 3 13 96 116 194 1.00e-17 75.9
MS.gene50297.t1 MTR_8g013005 61.017 59 20 1 69 124 1 59 1.09e-17 71.6
MS.gene50297.t1 MTR_7g079420 41.228 114 55 5 17 124 1037 1144 7.41e-16 72.8
MS.gene50297.t1 MTR_4g122840 39.370 127 39 3 1 124 173 264 1.48e-15 70.9
MS.gene50297.t1 MTR_2g048580 37.615 109 53 5 19 124 246 342 5.89e-12 61.2
MS.gene50297.t1 MTR_4g109290 60.377 53 18 1 75 124 136 188 3.10e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 28 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATACTGGTTTGTGGGTATT+TGG 0.151877 4.3:-74908527 MS.gene50297:CDS
CTAATGATACAATTGAAATA+TGG 0.264279 4.3:-74908472 MS.gene50297:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.267319 4.3:-74908508 MS.gene50297:CDS
ATGATAAAGGACAGTTATTA+AGG 0.300794 4.3:-74908298 MS.gene50297:CDS
ATAATCAGATGGACTATTAA+AGG 0.324115 4.3:+74908268 None:intergenic
ATCTGATTATGTCATGTATA+CGG 0.391652 4.3:-74908255 MS.gene50297:CDS
TTGGCGAAGTATAATGATAA+AGG 0.416108 4.3:-74908311 MS.gene50297:CDS
ATAGGGCGAAAGCAGGGTTC+AGG 0.417607 4.3:+74908383 None:intergenic
TGTTCAGAATCACTTGGAAT+AGG 0.431059 4.3:+74908563 None:intergenic
CACAGAGCATTAAGACATAC+TGG 0.435069 4.3:-74908542 MS.gene50297:CDS
AATACAATGTGCATTCATCT+TGG 0.441546 4.3:-74908439 MS.gene50297:CDS
GATATTATTGGAATAAGTCC+TGG 0.455002 4.3:-74908341 MS.gene50297:CDS
TAATGATACAATTGAAATAT+GGG 0.469242 4.3:-74908471 MS.gene50297:CDS
ATTAAGACATACTGGTTTGT+GGG 0.486970 4.3:-74908534 MS.gene50297:CDS
TGGAGAATTTCTCAGTCTAT+GGG 0.490268 4.3:-74908507 MS.gene50297:CDS
AATAAGTCCTGGGACTAGAT+TGG 0.496773 4.3:-74908330 MS.gene50297:CDS
CATTAAGACATACTGGTTTG+TGG 0.501728 4.3:-74908535 MS.gene50297:CDS
ATTTCTCAGTCTATGGGCTA+TGG 0.507502 4.3:-74908501 MS.gene50297:CDS
GAGTCTCTGCTTTCACTCCC+TGG 0.511723 4.3:-74908233 MS.gene50297:CDS
TAAACTTGCTCATTGTCACC+AGG 0.534960 4.3:+74908215 None:intergenic
AGAACATATAGGGCGAAAGC+AGG 0.538165 4.3:+74908376 None:intergenic
TACTTCGCCAATCTAGTCCC+AGG 0.568427 4.3:+74908323 None:intergenic
ATATTATTGGAATAAGTCCT+GGG 0.574033 4.3:-74908340 MS.gene50297:CDS
GCGAAAGCAGGGTTCAGGAA+TGG 0.589429 4.3:+74908388 None:intergenic
GTATACATGACATAATCAGA+TGG 0.633695 4.3:+74908257 None:intergenic
GAACATATAGGGCGAAAGCA+GGG 0.645510 4.3:+74908377 None:intergenic
GCTCTGTGTTCAGAATCACT+TGG 0.661251 4.3:+74908557 None:intergenic
AAACTTGCTCATTGTCACCA+GGG 0.667535 4.3:+74908216 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGATACAATTGAAATAT+GGG - chr4.3:74908309-74908328 MS.gene50297:CDS 15.0%
!! ACAAAAAATGGTGATATTAT+TGG - chr4.3:74908427-74908446 MS.gene50297:CDS 20.0%
!! CTAATGATACAATTGAAATA+TGG - chr4.3:74908308-74908327 MS.gene50297:CDS 20.0%
!!! CATTTTTTGTAGAACATATA+GGG + chr4.3:74908417-74908436 None:intergenic 20.0%
! ATATTATTGGAATAAGTCCT+GGG - chr4.3:74908440-74908459 MS.gene50297:CDS 25.0%
! ATCTGATTATGTCATGTATA+CGG - chr4.3:74908525-74908544 MS.gene50297:CDS 25.0%
! ATGATAAAGGACAGTTATTA+AGG - chr4.3:74908482-74908501 MS.gene50297:CDS 25.0%
! CCTATATGTTCTACAAAAAA+TGG - chr4.3:74908415-74908434 MS.gene50297:CDS 25.0%
!!! ATAATCAGATGGACTATTAA+AGG + chr4.3:74908515-74908534 None:intergenic 25.0%
!!! CCATTTTTTGTAGAACATAT+AGG + chr4.3:74908418-74908437 None:intergenic 25.0%
AATACAATGTGCATTCATCT+TGG - chr4.3:74908341-74908360 MS.gene50297:CDS 30.0%
GATATTATTGGAATAAGTCC+TGG - chr4.3:74908439-74908458 MS.gene50297:CDS 30.0%
GTATACATGACATAATCAGA+TGG + chr4.3:74908526-74908545 None:intergenic 30.0%
TTGGCGAAGTATAATGATAA+AGG - chr4.3:74908469-74908488 MS.gene50297:CDS 30.0%
!! ATTAAGACATACTGGTTTGT+GGG - chr4.3:74908246-74908265 MS.gene50297:CDS 30.0%
TGGAGAATTTCTCAGTCTAT+GGG - chr4.3:74908273-74908292 MS.gene50297:CDS 35.0%
TGTTCAGAATCACTTGGAAT+AGG + chr4.3:74908220-74908239 None:intergenic 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr4.3:74908272-74908291 MS.gene50297:CDS 35.0%
!! CATTAAGACATACTGGTTTG+TGG - chr4.3:74908245-74908264 MS.gene50297:CDS 35.0%
AATAAGTCCTGGGACTAGAT+TGG - chr4.3:74908450-74908469 MS.gene50297:CDS 40.0%
! AAACTTGCTCATTGTCACCA+GGG + chr4.3:74908567-74908586 None:intergenic 40.0%
! ATTTCTCAGTCTATGGGCTA+TGG - chr4.3:74908279-74908298 MS.gene50297:CDS 40.0%
! CACAGAGCATTAAGACATAC+TGG - chr4.3:74908238-74908257 MS.gene50297:CDS 40.0%
! TAAACTTGCTCATTGTCACC+AGG + chr4.3:74908568-74908587 None:intergenic 40.0%
!! CATACTGGTTTGTGGGTATT+TGG - chr4.3:74908253-74908272 MS.gene50297:CDS 40.0%
AGAACATATAGGGCGAAAGC+AGG + chr4.3:74908407-74908426 None:intergenic 45.0%
GAACATATAGGGCGAAAGCA+GGG + chr4.3:74908406-74908425 None:intergenic 45.0%
GCTCTGTGTTCAGAATCACT+TGG + chr4.3:74908226-74908245 None:intergenic 45.0%
TACTTCGCCAATCTAGTCCC+AGG + chr4.3:74908460-74908479 None:intergenic 50.0%
ATAGGGCGAAAGCAGGGTTC+AGG + chr4.3:74908400-74908419 None:intergenic 55.0%
GAGTCTCTGCTTTCACTCCC+TGG - chr4.3:74908547-74908566 MS.gene50297:CDS 55.0%
GCGAAAGCAGGGTTCAGGAA+TGG + chr4.3:74908395-74908414 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 74908214 74908588 74908214 ID=MS.gene50297
chr4.3 mRNA 74908214 74908588 74908214 ID=MS.gene50297.t1;Parent=MS.gene50297
chr4.3 exon 74908214 74908588 74908214 ID=MS.gene50297.t1.exon1;Parent=MS.gene50297.t1
chr4.3 CDS 74908214 74908588 74908214 ID=cds.MS.gene50297.t1;Parent=MS.gene50297.t1
Gene Sequence

>MS.gene50297

ATGCCTATTCCAAGTGATTCTGAACACAGAGCATTAAGACATACTGGTTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGGCTATGGATACTGCTAATGATACAATTGAAATATGGGTGATGAAAGAATACAATGTGCATTCATCTTGGACTAAAACTCTTGTTCTTTCTGTTGATACCATTCCTGAACCCTGCTTTCGCCCTATATGTTCTACAAAAAATGGTGATATTATTGGAATAAGTCCTGGGACTAGATTGGCGAAGTATAATGATAAAGGACAGTTATTAAGGCATCGCTCCTTTAATAGTCCATCTGATTATGTCATGTATACGGAGTCTCTGCTTTCACTCCCTGGTGACAATGAGCAAGTTTAA

Protein sequence

>MS.gene50297.t1

MPIPSDSEHRALRHTGLWVFGEFLSLWAMDTANDTIEIWVMKEYNVHSSWTKTLVLSVDTIPEPCFRPICSTKNGDIIGISPGTRLAKYNDKGQLLRHRSFNSPSDYVMYTESLLSLPGDNEQV