AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77704

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77704.t1 MTR_4g023090 75.330 227 38 3 6 219 165 386 3.53e-115 334
MS.gene77704.t1 MTR_4g023380 70.638 235 32 2 4 219 163 379 1.19e-112 327
MS.gene77704.t1 MTR_4g023130 70.370 216 50 5 4 218 110 312 1.55e-97 286
MS.gene77704.t1 MTR_4g023080 70.233 215 50 5 5 218 152 353 3.99e-95 282
MS.gene77704.t1 MTR_4g023070 61.135 229 73 3 4 218 145 371 1.73e-87 263
MS.gene77704.t1 MTR_4g021785 64.977 217 61 5 4 218 151 354 2.45e-86 259
MS.gene77704.t1 MTR_4g023120 59.130 230 76 4 4 219 145 370 2.15e-82 250
MS.gene77704.t1 MTR_4g023440 60.930 215 39 4 5 219 163 332 2.72e-79 241
MS.gene77704.t1 MTR_3g103020 60.550 218 74 7 7 218 166 377 1.38e-77 238
MS.gene77704.t1 MTR_6g060650 56.682 217 83 6 5 218 164 372 2.43e-74 229
MS.gene77704.t1 MTR_4g116320 59.906 212 74 6 7 212 47 253 4.14e-74 225
MS.gene77704.t1 MTR_4g117720 58.744 223 76 7 5 218 162 377 1.33e-73 228
MS.gene77704.t1 MTR_4g023150 59.259 216 74 6 5 217 154 358 8.33e-72 222
MS.gene77704.t1 MTR_4g023190 60.185 216 71 7 5 217 154 357 1.26e-71 222
MS.gene77704.t1 MTR_4g023200 58.333 216 76 6 5 217 154 358 1.11e-70 219
MS.gene77704.t1 MTR_8g433030 58.768 211 78 7 6 212 132 337 1.42e-70 219
MS.gene77704.t1 MTR_8g032390 58.768 211 78 7 6 212 165 370 4.88e-70 218
MS.gene77704.t1 MTR_4g109270 56.682 217 77 7 7 218 159 363 8.71e-70 218
MS.gene77704.t1 MTR_3g034180 54.825 228 82 8 5 219 270 489 5.97e-68 216
MS.gene77704.t1 MTR_4g116330 55.505 218 84 6 6 218 170 379 8.67e-68 213
MS.gene77704.t1 MTR_4g122850 55.856 222 84 9 5 218 150 365 7.07e-67 210
MS.gene77704.t1 MTR_8g040935 54.884 215 79 8 14 218 1 207 2.58e-66 203
MS.gene77704.t1 MTR_4g109280 53.881 219 87 6 6 218 148 358 1.64e-65 206
MS.gene77704.t1 MTR_4g094365 54.545 220 85 8 5 219 155 364 4.69e-64 202
MS.gene77704.t1 MTR_4g117850 54.338 219 85 7 7 216 164 376 4.87e-64 203
MS.gene77704.t1 MTR_3g034130 53.659 205 79 5 5 198 156 355 1.90e-63 201
MS.gene77704.t1 MTR_3g034160 55.000 220 80 7 6 212 157 370 6.24e-63 200
MS.gene77704.t1 MTR_4g117770 56.621 219 80 9 7 219 158 367 1.60e-62 199
MS.gene77704.t1 MTR_4g117810 56.621 219 80 9 7 219 158 367 1.60e-62 199
MS.gene77704.t1 MTR_8g433040 54.795 219 78 8 5 219 163 364 2.59e-62 198
MS.gene77704.t1 MTR_8g032380 54.795 219 78 8 5 219 163 364 2.59e-62 198
MS.gene77704.t1 MTR_6g463430 52.607 211 86 6 14 219 1 202 6.83e-62 192
MS.gene77704.t1 MTR_4g118380 53.299 197 78 4 5 192 166 357 3.90e-60 192
MS.gene77704.t1 MTR_4g117780 53.881 219 86 8 7 219 158 367 5.01e-59 190
MS.gene77704.t1 MTR_5g070130 49.778 225 98 6 5 218 156 376 6.90e-58 187
MS.gene77704.t1 MTR_5g070080 52.968 219 91 8 5 218 174 385 4.78e-57 185
MS.gene77704.t1 MTR_5g070088 48.636 220 105 5 5 219 170 386 3.84e-55 180
MS.gene77704.t1 MTR_4g117870 48.571 210 67 7 5 212 92 262 2.65e-52 169
MS.gene77704.t1 MTR_4g118400 47.143 210 86 4 5 211 107 294 5.92e-52 169
MS.gene77704.t1 MTR_5g097210 50.463 216 90 9 5 211 150 357 2.81e-50 167
MS.gene77704.t1 MTR_1g055195 46.018 226 81 7 5 211 269 472 3.68e-49 167
MS.gene77704.t1 MTR_1g055175 46.860 207 81 9 27 218 8 200 1.18e-48 158
MS.gene77704.t1 MTR_1g055135 45.022 231 98 8 5 218 156 374 1.68e-48 163
MS.gene77704.t1 MTR_1g055250 44.175 206 101 7 5 204 56 253 4.92e-46 155
MS.gene77704.t1 MTR_4g116330 45.413 218 67 6 6 218 170 340 4.07e-45 153
MS.gene77704.t1 MTR_4g117750 47.511 221 96 9 5 211 139 353 6.84e-44 153
MS.gene77704.t1 MTR_4g117750 51.852 135 52 4 91 215 350 481 1.64e-29 114
MS.gene77704.t1 MTR_1g055165 49.171 181 75 7 42 218 150 317 6.95e-44 149
MS.gene77704.t1 MTR_4g117530 45.070 213 73 6 7 218 83 252 8.33e-44 147
MS.gene77704.t1 MTR_4g118370 43.721 215 89 9 5 211 64 254 5.47e-43 145
MS.gene77704.t1 MTR_7g079370 43.379 219 105 7 5 211 139 350 7.58e-42 145
MS.gene77704.t1 MTR_4g121580 45.455 209 75 9 5 207 164 339 1.72e-41 145
MS.gene77704.t1 MTR_7g079400 42.222 225 109 8 5 215 118 335 5.24e-39 137
MS.gene77704.t1 MTR_5g023000 64.646 99 31 2 122 218 2 98 3.91e-34 117
MS.gene77704.t1 MTR_4g118360 54.762 126 46 5 91 211 1 120 1.81e-33 117
MS.gene77704.t1 MTR_8g102520 39.252 214 113 8 8 211 110 316 2.29e-31 117
MS.gene77704.t1 MTR_4g114700 38.389 211 71 3 6 208 59 218 6.11e-31 114
MS.gene77704.t1 MTR_4g122860 43.548 186 68 8 34 211 158 314 2.69e-30 114
MS.gene77704.t1 MTR_4g117430 50.862 116 49 2 4 119 48 155 7.48e-28 103
MS.gene77704.t1 MTR_4g023430 65.476 84 22 2 141 219 141 222 9.91e-28 105
MS.gene77704.t1 MTR_8g098830 38.462 195 103 8 24 211 31 215 1.45e-27 105
MS.gene77704.t1 MTR_7g079420 38.182 220 102 10 8 215 945 1142 4.04e-26 105
MS.gene77704.t1 MTR_8g013010 50.413 121 47 4 5 121 203 314 5.45e-26 102
MS.gene77704.t1 MTR_7g079450 45.652 138 63 4 77 211 3 131 1.95e-25 97.1
MS.gene77704.t1 MTR_2g048580 35.468 203 104 9 25 216 155 341 8.84e-23 94.7
MS.gene77704.t1 MTR_4g023100 74.576 59 13 1 161 219 63 119 3.43e-21 85.5
MS.gene77704.t1 MTR_7g117720 59.302 86 29 2 132 211 186 271 5.27e-21 88.6
MS.gene77704.t1 MTR_6g034720 47.253 91 46 2 5 93 80 170 1.27e-17 78.6
MS.gene77704.t1 MTR_8g013005 63.793 58 17 2 163 218 3 58 1.62e-15 68.6
MS.gene77704.t1 MTR_4g116340 55.844 77 32 2 6 81 172 247 1.69e-15 73.2
MS.gene77704.t1 MTR_4g109290 64.286 56 16 2 165 218 134 187 8.53e-14 67.4
MS.gene77704.t1 MTR_1g055145 48.052 77 35 2 59 135 131 202 9.44e-13 66.2
MS.gene77704.t1 MTR_4g061340 33.571 140 69 7 23 154 203 326 2.24e-12 65.5
MS.gene77704.t1 MTR_1g094210 26.344 186 116 7 9 188 206 376 1.20e-11 63.5
MS.gene77704.t1 MTR_2g021800 25.789 190 128 5 29 210 228 412 2.52e-11 62.8
MS.gene77704.t1 MTR_8g099460 26.087 207 135 8 3 207 161 351 6.53e-11 61.2
MS.gene77704.t1 MTR_1g094210 25.701 214 125 9 9 209 206 398 9.68e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 53 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGTTGCCTTTGATTTAA+TGG 0.113056 4.4:+67975571 MS.gene77704:CDS
CATTCTGGTTTGTGGGTATT+TGG 0.171406 4.4:+67975650 MS.gene77704:CDS
AGAAAAGGGTTGCTATTTAA+TGG 0.226317 4.4:+67975503 MS.gene77704:CDS
TTGGAGAATTTCTCAGTATA+TGG 0.239627 4.4:+67975669 MS.gene77704:CDS
TTGGCTTTGCGTCGTAATTT+AGG 0.260116 4.4:+67975539 MS.gene77704:CDS
ATGATAAAGGACAGTTATTA+AGG 0.300794 4.4:+67975885 MS.gene77704:CDS
GAAAAGGGTTGCTATTTAAT+GGG 0.312663 4.4:+67975504 MS.gene77704:CDS
ATAATGATACAGTTGAAATA+TGG 0.320851 4.4:+67975711 MS.gene77704:CDS
CACAAAGCATTGGTACATTC+TGG 0.326889 4.4:+67975635 MS.gene77704:CDS
TATTTAATGGGGCTATTCAT+TGG 0.360740 4.4:+67975516 MS.gene77704:CDS
GCTTTGTGGTCAACATCATT+TGG 0.369346 4.4:-67975620 None:intergenic
ATTAGCTGCGGTTGGATCAT+AGG 0.393069 4.4:-67975384 None:intergenic
GCCATCCCTTCTCGCAGAAA+AGG 0.395590 4.4:+67975488 MS.gene77704:CDS
TGGAGAATTTCTCAGTATAT+GGG 0.402920 4.4:+67975670 MS.gene77704:CDS
TGCTCGTTGTCACCAGGGCC+AGG 0.412802 4.4:-67975971 None:intergenic
CATTGGTACATTCTGGTTTG+TGG 0.418702 4.4:+67975642 MS.gene77704:CDS
ATTGGTACATTCTGGTTTGT+GGG 0.430276 4.4:+67975643 MS.gene77704:CDS
TAATGGGGCTATTCATTGGT+TGG 0.433983 4.4:+67975520 MS.gene77704:CDS
CGAGAAGGGATGGCAACATA+AGG 0.436687 4.4:-67975479 None:intergenic
TAAATTTGCTCGTTGTCACC+AGG 0.457925 4.4:-67975977 None:intergenic
ATCCGAAGTTGTCATGTATA+CGG 0.460650 4.4:+67975931 MS.gene77704:CDS
CTGCTTTCACTCCCTGGCCC+TGG 0.461764 4.4:+67975959 MS.gene77704:CDS
TTCGGATGGACTATTATAGA+AGG 0.465105 4.4:-67975915 None:intergenic
AATACAAAGTGAATTCATCT+TGG 0.472564 4.4:+67975744 MS.gene77704:CDS
TTTCCTTTCCATTAAATCAA+AGG 0.474723 4.4:-67975579 None:intergenic
GAGTCTCTGCTTTCACTCCC+TGG 0.477411 4.4:+67975953 MS.gene77704:CDS
CCAGTCAAGAGATGATTACT+TGG 0.479956 4.4:+67975349 MS.gene77704:intron
CAGAATGTACCAATGCTTTG+TGG 0.480428 4.4:-67975634 None:intergenic
CCATCCCTTCTCGCAGAAAA+GGG 0.490399 4.4:+67975489 MS.gene77704:CDS
TTGGTGAAGTATAATGATAA+AGG 0.492510 4.4:+67975872 MS.gene77704:CDS
GAAACATCATTAGCTGCGGT+TGG 0.496696 4.4:-67975392 None:intergenic
AAGAAATCGTGGTACTAGAT+TGG 0.508666 4.4:+67975853 MS.gene77704:CDS
ATTTCTCAGTATATGGGCCA+TGG 0.520677 4.4:+67975676 MS.gene77704:CDS
TGGTCAACATCATTTGGAAG+AGG 0.521206 4.4:-67975614 None:intergenic
GCTCGTTGTCACCAGGGCCA+GGG 0.528579 4.4:-67975970 None:intergenic
TAATGATACAGTTGAAATAT+GGG 0.533072 4.4:+67975712 MS.gene77704:CDS
TTGCCTTTGATTTAATGGAA+AGG 0.533360 4.4:+67975576 MS.gene77704:CDS
TAATACATGGAAAGAAAATG+AGG 0.536958 4.4:+67975445 MS.gene77704:CDS
TTGAAAGTAATGATCATGAA+TGG 0.538193 4.4:-67975795 None:intergenic
TCTCATTTAGAGATAATACA+TGG 0.548869 4.4:+67975432 MS.gene77704:CDS
GAGAAGGGATGGCAACATAA+GGG 0.552037 4.4:-67975478 None:intergenic
GTCAAGAGATGATTACTTGG+TGG 0.564802 4.4:+67975352 MS.gene77704:intron
ATTATCATTATCATAATCCA+TGG 0.571255 4.4:-67975693 None:intergenic
TGATGTTGACCACAAAGCAT+TGG 0.583728 4.4:+67975625 MS.gene77704:CDS
AGGGATGGCAACATAAGGGA+AGG 0.593796 4.4:-67975474 None:intergenic
AAATTTGCTCGTTGTCACCA+GGG 0.612054 4.4:-67975976 None:intergenic
AAAAGGGTTGCTATTTAATG+GGG 0.619929 4.4:+67975505 MS.gene77704:CDS
GGGATGGCAACATAAGGGAA+GGG 0.620262 4.4:-67975473 None:intergenic
AATACATGGAAAGAAAATGA+GGG 0.628611 4.4:+67975446 MS.gene77704:CDS
CTCCGTATACATGACAACTT+CGG 0.636118 4.4:-67975933 None:intergenic
GTATACATGACAACTTCGGA+TGG 0.641195 4.4:-67975929 None:intergenic
GATATTGTTGGAAGAAATCG+TGG 0.669142 4.4:+67975842 MS.gene77704:CDS
TGTTGAAACATCATTAGCTG+CGG 0.713336 4.4:-67975396 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATGATACAGTTGAAATA+TGG + chr4.4:67975711-67975730 MS.gene77704:CDS 20.0%
!! ATTATCATTATCATAATCCA+TGG - chr4.4:67975696-67975715 None:intergenic 20.0%
!! ATTATGGAAGCAAAAAATTA+AGG - chr4.4:67975154-67975173 None:intergenic 20.0%
!! TAATGATACAGTTGAAATAT+GGG + chr4.4:67975712-67975731 MS.gene77704:CDS 20.0%
!! TCAACTAGAAAATAAGATTA+TGG - chr4.4:67975170-67975189 None:intergenic 20.0%
!!! TAATTGTTTTTTTAATCCAG+TGG - chr4.4:67975261-67975280 None:intergenic 20.0%
!!! TATTTTCTAGTTGAAATTAG+AGG + chr4.4:67975176-67975195 MS.gene77704:intron 20.0%
! AAAATGCGAATTTGCAAAAT+GGG - chr4.4:67975018-67975037 None:intergenic 25.0%
! AATACAAAGTGAATTCATCT+TGG + chr4.4:67975744-67975763 MS.gene77704:CDS 25.0%
! AATACATGGAAAGAAAATGA+GGG + chr4.4:67975446-67975465 MS.gene77704:CDS 25.0%
! ATGATAAAGGACAGTTATTA+AGG + chr4.4:67975885-67975904 MS.gene77704:CDS 25.0%
! CCTATACATTCTACAAAAAT+TGG + chr4.4:67975818-67975837 MS.gene77704:CDS 25.0%
! TAATACATGGAAAGAAAATG+AGG + chr4.4:67975445-67975464 MS.gene77704:CDS 25.0%
! TCTCATGAAATTTGATCAAT+AGG + chr4.4:67975089-67975108 MS.gene77704:intron 25.0%
! TCTCATTTAGAGATAATACA+TGG + chr4.4:67975432-67975451 MS.gene77704:CDS 25.0%
! TTGAAAGTAATGATCATGAA+TGG - chr4.4:67975798-67975817 None:intergenic 25.0%
! TTGGTGAAGTATAATGATAA+AGG + chr4.4:67975872-67975891 MS.gene77704:CDS 25.0%
! TTTCCTTTCCATTAAATCAA+AGG - chr4.4:67975582-67975601 None:intergenic 25.0%
!! ACAAAAATTGGTGATATTGT+TGG + chr4.4:67975830-67975849 MS.gene77704:CDS 25.0%
!! CACATTCATATACATTGTTT+TGG + chr4.4:67975320-67975339 MS.gene77704:intron 25.0%
!! CCAATTTTTGTAGAATGTAT+AGG - chr4.4:67975821-67975840 None:intergenic 25.0%
AAATGCGAATTTGCAAAATG+GGG - chr4.4:67975017-67975036 None:intergenic 30.0%
AAATTAGAGGTTCATGTAGA+GGG + chr4.4:67975189-67975208 MS.gene77704:intron 30.0%
GAAAATGCGAATTTGCAAAA+TGG - chr4.4:67975019-67975038 None:intergenic 30.0%
GATCAAATTTCATGAGATAG+AGG - chr4.4:67975086-67975105 None:intergenic 30.0%
GTGACAATATGATGCTAAAT+TGG - chr4.4:67975303-67975322 None:intergenic 30.0%
TATTTAATGGGGCTATTCAT+TGG + chr4.4:67975516-67975535 MS.gene77704:CDS 30.0%
TGGAGAATTTCTCAGTATAT+GGG + chr4.4:67975670-67975689 MS.gene77704:CDS 30.0%
TTGGAGAATTTCTCAGTATA+TGG + chr4.4:67975669-67975688 MS.gene77704:CDS 30.0%
! CATTGTTGCCTTTGATTTAA+TGG + chr4.4:67975571-67975590 MS.gene77704:CDS 30.0%
! TTGCCTTTGATTTAATGGAA+AGG + chr4.4:67975576-67975595 MS.gene77704:CDS 30.0%
!! AAAAGGGTTGCTATTTAATG+GGG + chr4.4:67975505-67975524 MS.gene77704:CDS 30.0%
!! AGAAAAGGGTTGCTATTTAA+TGG + chr4.4:67975503-67975522 MS.gene77704:CDS 30.0%
!! GAAAAGGGTTGCTATTTAAT+GGG + chr4.4:67975504-67975523 MS.gene77704:CDS 30.0%
!!! TGTTTTTTTAATCCAGTGGA+TGG - chr4.4:67975257-67975276 None:intergenic 30.0%
AAGAAATCGTGGTACTAGAT+TGG + chr4.4:67975853-67975872 MS.gene77704:CDS 35.0%
ATATGTAGATGTTTGGACGA+CGG - chr4.4:67975229-67975248 None:intergenic 35.0%
ATCCGAAGTTGTCATGTATA+CGG + chr4.4:67975931-67975950 MS.gene77704:CDS 35.0%
GAAATTAGAGGTTCATGTAG+AGG + chr4.4:67975188-67975207 MS.gene77704:intron 35.0%
GATATTGTTGGAAGAAATCG+TGG + chr4.4:67975842-67975861 MS.gene77704:CDS 35.0%
GATGCTAAATTGGAACGAAA+AGG - chr4.4:67975293-67975312 None:intergenic 35.0%
GGAGATATGCACTTAATTCT+AGG - chr4.4:67975065-67975084 None:intergenic 35.0%
TGTTGAAACATCATTAGCTG+CGG - chr4.4:67975399-67975418 None:intergenic 35.0%
!! ATTGGTACATTCTGGTTTGT+GGG + chr4.4:67975643-67975662 MS.gene77704:CDS 35.0%
!! TTCGGATGGACTATTATAGA+AGG - chr4.4:67975918-67975937 None:intergenic 35.0%
ATTTCTCAGTATATGGGCCA+TGG + chr4.4:67975676-67975695 MS.gene77704:CDS 40.0%
CAGAATGTACCAATGCTTTG+TGG - chr4.4:67975637-67975656 None:intergenic 40.0%
CCAAGTAATCATCTCTTGAC+TGG - chr4.4:67975352-67975371 None:intergenic 40.0%
CCAGTCAAGAGATGATTACT+TGG + chr4.4:67975349-67975368 MS.gene77704:intron 40.0%
CTCCGTATACATGACAACTT+CGG - chr4.4:67975936-67975955 None:intergenic 40.0%
GCTTTGTGGTCAACATCATT+TGG - chr4.4:67975623-67975642 None:intergenic 40.0%
GGACTCCATATGTAGATGTT+TGG - chr4.4:67975236-67975255 None:intergenic 40.0%
GTATACATGACAACTTCGGA+TGG - chr4.4:67975932-67975951 None:intergenic 40.0%
GTCAAGAGATGATTACTTGG+TGG + chr4.4:67975352-67975371 MS.gene77704:intron 40.0%
GTCGTCCAAACATCTACATA+TGG + chr4.4:67975228-67975247 MS.gene77704:intron 40.0%
TAATGGGGCTATTCATTGGT+TGG + chr4.4:67975520-67975539 MS.gene77704:CDS 40.0%
TGATGTTGACCACAAAGCAT+TGG + chr4.4:67975625-67975644 MS.gene77704:CDS 40.0%
TGGTCAACATCATTTGGAAG+AGG - chr4.4:67975617-67975636 None:intergenic 40.0%
! AAATTTGCTCGTTGTCACCA+GGG - chr4.4:67975979-67975998 None:intergenic 40.0%
! TAAATTTGCTCGTTGTCACC+AGG - chr4.4:67975980-67975999 None:intergenic 40.0%
!! CACAAAGCATTGGTACATTC+TGG + chr4.4:67975635-67975654 MS.gene77704:CDS 40.0%
!! CATTCTGGTTTGTGGGTATT+TGG + chr4.4:67975650-67975669 MS.gene77704:CDS 40.0%
!! CATTGGTACATTCTGGTTTG+TGG + chr4.4:67975642-67975661 MS.gene77704:CDS 40.0%
!! TTGGCTTTGCGTCGTAATTT+AGG + chr4.4:67975539-67975558 MS.gene77704:CDS 40.0%
ATTAGCTGCGGTTGGATCAT+AGG - chr4.4:67975387-67975406 None:intergenic 45.0%
GAAACATCATTAGCTGCGGT+TGG - chr4.4:67975395-67975414 None:intergenic 45.0%
GAGAAGGGATGGCAACATAA+GGG - chr4.4:67975481-67975500 None:intergenic 45.0%
TACATATGGAGTCCATCCAC+TGG + chr4.4:67975242-67975261 MS.gene77704:intron 45.0%
TTGGAACGAAAAGGAGACAC+AGG - chr4.4:67975284-67975303 None:intergenic 45.0%
! AATTCTAGGAGTGTGCGTTC+TGG - chr4.4:67975051-67975070 None:intergenic 45.0%
AGGGATGGCAACATAAGGGA+AGG - chr4.4:67975477-67975496 None:intergenic 50.0%
CCATCCCTTCTCGCAGAAAA+GGG + chr4.4:67975489-67975508 MS.gene77704:CDS 50.0%
CGAGAAGGGATGGCAACATA+AGG - chr4.4:67975482-67975501 None:intergenic 50.0%
GGGATGGCAACATAAGGGAA+GGG - chr4.4:67975476-67975495 None:intergenic 50.0%
! AGCAACCCTTTTCTGCGAGA+AGG - chr4.4:67975497-67975516 None:intergenic 50.0%
! GCAACCCTTTTCTGCGAGAA+GGG - chr4.4:67975496-67975515 None:intergenic 50.0%
GAGTCTCTGCTTTCACTCCC+TGG + chr4.4:67975953-67975972 MS.gene77704:CDS 55.0%
GCCATCCCTTCTCGCAGAAA+AGG + chr4.4:67975488-67975507 MS.gene77704:CDS 55.0%
! CCCTTTTCTGCGAGAAGGGA+TGG - chr4.4:67975492-67975511 None:intergenic 55.0%
! TCTAGGAGTGTGCGTTCTGG+TGG - chr4.4:67975048-67975067 None:intergenic 55.0%
CTGCTTTCACTCCCTGGCCC+TGG + chr4.4:67975959-67975978 MS.gene77704:CDS 65.0%
GCTCGTTGTCACCAGGGCCA+GGG - chr4.4:67975973-67975992 None:intergenic 65.0%
TGCTCGTTGTCACCAGGGCC+AGG - chr4.4:67975974-67975993 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 67974994 67976000 67974994 ID=MS.gene77704
chr4.4 mRNA 67974994 67976000 67974994 ID=MS.gene77704.t1;Parent=MS.gene77704
chr4.4 exon 67974994 67975005 67974994 ID=MS.gene77704.t1.exon1;Parent=MS.gene77704.t1
chr4.4 CDS 67974994 67975005 67974994 ID=cds.MS.gene77704.t1;Parent=MS.gene77704.t1
chr4.4 exon 67975353 67976000 67975353 ID=MS.gene77704.t1.exon2;Parent=MS.gene77704.t1
chr4.4 CDS 67975353 67976000 67975353 ID=cds.MS.gene77704.t1;Parent=MS.gene77704.t1
Gene Sequence

>MS.gene77704

ATGTGGCTGTCGGTGAAGATCCCCATTTTGCAAATTCGCATTTTCAACTCACCACCAGAACGCACACTCCTAGAATTAAGTGCATATCTCCTCTATCTCATGAAATTTGATCAATAGGTTTTGAAGCATTTCTTAACGATGATAGTGCTTCGCTGAACCTTAATTTTTTGCTTCCATAATCTTATTTTCTAGTTGAAATTAGAGGTTCATGTAGAGGGTTTATTCTTTTGTACCGTCGTCCAAACATCTACATATGGAGTCCATCCACTGGATTAAAAAAACAATTACCTGTGTCTCCTTTTCGTTCCAATTTAGCATCATATTGTCACATTCATATACATTGTTTTGGTTATGACCAGTCAAGAGATGATTACTTGGTGGTTGTATTGTCCTATGATCCAACCGCAGCTAATGATGTTTCAACACATTTGAAGTTTTTCTCATTTAGAGATAATACATGGAAAGAAAATGAGGGCACTCCCTTCCCTTATGTTGCCATCCCTTCTCGCAGAAAAGGGTTGCTATTTAATGGGGCTATTCATTGGTTGGCTTTGCGTCGTAATTTAGGTTTGATTGTCATTGTTGCCTTTGATTTAATGGAAAGGAAATTATTTGAGATGCCTCTTCCAAATGATGTTGACCACAAAGCATTGGTACATTCTGGTTTGTGGGTATTTGGAGAATTTCTCAGTATATGGGCCATGGATTATGATAATGATAATGATACAGTTGAAATATGGGTGATGAAAGAATACAAAGTGAATTCATCTTGGACTAAAACTCTTATTCTTTCTGTTGATGCCATTCATGATCATTACTTTCAACCTATACATTCTACAAAAATTGGTGATATTGTTGGAAGAAATCGTGGTACTAGATTGGTGAAGTATAATGATAAAGGACAGTTATTAAGGCATTGCTCCTTCTATAATAGTCCATCCGAAGTTGTCATGTATACGGAGTCTCTGCTTTCACTCCCTGGCCCTGGTGACAACGAGCAAATTTAA
Protein sequence

>MS.gene77704.t1

MWLSSRDDYLVVVLSYDPTAANDVSTHLKFFSFRDNTWKENEGTPFPYVAIPSRRKGLLFNGAIHWLALRRNLGLIVIVAFDLMERKLFEMPLPNDVDHKALVHSGLWVFGEFLSIWAMDYDNDNDTVEIWVMKEYKVNSSWTKTLILSVDAIHDHYFQPIHSTKIGDIVGRNRGTRLVKYNDKGQLLRHCSFYNSPSEVVMYTESLLSLPGPGDNEQI