AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50339

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50339.t1 MTR_4g023120 90.747 281 26 0 28 308 90 370 0.0 536
MS.gene50339.t1 MTR_4g023070 90.000 280 28 0 28 307 90 369 0.0 527
MS.gene50339.t1 MTR_4g023090 62.500 288 96 4 28 308 104 386 7.31e-122 355
MS.gene50339.t1 MTR_4g023130 58.716 327 90 6 3 307 9 312 2.80e-118 343
MS.gene50339.t1 MTR_4g021785 61.484 283 84 4 28 308 96 355 7.01e-116 338
MS.gene50339.t1 MTR_4g023380 55.988 334 76 7 2 308 90 379 8.10e-113 331
MS.gene50339.t1 MTR_4g023080 61.922 281 83 4 28 307 96 353 8.73e-112 328
MS.gene50339.t1 MTR_4g023200 56.383 282 100 5 28 306 97 358 2.97e-98 293
MS.gene50339.t1 MTR_4g023150 56.383 282 100 5 28 306 97 358 4.59e-98 293
MS.gene50339.t1 MTR_4g023190 57.092 282 97 6 28 306 97 357 1.06e-97 292
MS.gene50339.t1 MTR_3g103020 55.137 292 100 9 28 308 107 378 3.37e-92 279
MS.gene50339.t1 MTR_4g117720 48.991 347 115 12 1 305 49 375 3.32e-90 274
MS.gene50339.t1 MTR_4g023440 50.171 293 91 3 16 308 95 332 5.07e-88 266
MS.gene50339.t1 MTR_4g094365 53.125 288 106 8 30 308 97 364 2.00e-86 263
MS.gene50339.t1 MTR_6g060650 51.590 283 110 8 33 307 109 372 4.49e-85 260
MS.gene50339.t1 MTR_4g116330 52.364 275 108 7 36 305 121 377 5.62e-84 258
MS.gene50339.t1 MTR_4g109280 51.916 287 114 7 28 308 91 359 6.51e-83 254
MS.gene50339.t1 MTR_4g117850 50.694 288 111 9 29 305 109 376 5.20e-81 250
MS.gene50339.t1 MTR_8g032390 46.705 349 120 12 3 303 40 370 1.95e-79 246
MS.gene50339.t1 MTR_4g117780 49.306 288 118 7 30 308 99 367 5.03e-77 239
MS.gene50339.t1 MTR_3g034130 52.174 276 100 9 28 289 98 355 3.48e-76 238
MS.gene50339.t1 MTR_8g433040 48.958 288 112 7 29 308 104 364 6.65e-76 236
MS.gene50339.t1 MTR_8g032380 48.958 288 112 7 29 308 104 364 6.65e-76 236
MS.gene50339.t1 MTR_4g122850 49.667 300 117 13 23 308 85 364 2.44e-75 236
MS.gene50339.t1 MTR_5g097210 50.172 291 114 11 23 304 91 359 1.90e-74 233
MS.gene50339.t1 MTR_3g034180 49.320 294 120 9 28 308 212 489 3.84e-74 236
MS.gene50339.t1 MTR_4g117770 48.966 290 120 7 28 308 97 367 9.06e-74 231
MS.gene50339.t1 MTR_4g117810 48.966 290 120 7 28 308 97 367 9.06e-74 231
MS.gene50339.t1 MTR_8g433030 45.872 327 122 10 3 303 40 337 1.77e-73 229
MS.gene50339.t1 MTR_3g034160 50.512 293 112 10 28 305 98 372 3.17e-73 230
MS.gene50339.t1 MTR_4g118380 49.442 269 104 7 29 283 107 357 2.70e-72 227
MS.gene50339.t1 MTR_4g117870 46.831 284 97 9 28 308 35 267 3.40e-71 221
MS.gene50339.t1 MTR_4g109270 47.766 291 120 9 28 308 96 364 1.52e-70 223
MS.gene50339.t1 MTR_1g055135 46.416 293 127 9 28 308 101 375 6.76e-69 219
MS.gene50339.t1 MTR_5g070080 46.528 288 119 10 28 303 116 380 7.16e-66 211
MS.gene50339.t1 MTR_5g070130 44.178 292 133 7 28 305 99 374 2.83e-65 210
MS.gene50339.t1 MTR_1g055195 44.483 290 120 8 28 305 215 475 4.59e-65 212
MS.gene50339.t1 MTR_5g070088 43.662 284 142 5 28 306 113 383 1.72e-64 208
MS.gene50339.t1 MTR_4g116330 45.091 275 89 7 36 305 121 338 1.78e-63 204
MS.gene50339.t1 MTR_4g116320 45.806 310 98 7 3 305 9 255 2.72e-63 201
MS.gene50339.t1 MTR_4g118400 44.404 277 106 8 29 301 61 293 8.09e-63 201
MS.gene50339.t1 MTR_4g121580 45.965 285 99 11 33 307 109 348 1.26e-60 198
MS.gene50339.t1 MTR_6g463430 53.431 204 74 4 109 308 16 202 1.80e-57 184
MS.gene50339.t1 MTR_8g040935 50.952 210 82 5 107 308 12 208 1.73e-56 182
MS.gene50339.t1 MTR_4g117750 43.945 289 130 9 29 304 86 355 2.01e-55 187
MS.gene50339.t1 MTR_4g117750 52.033 123 41 3 188 306 373 481 1.77e-25 106
MS.gene50339.t1 MTR_7g079370 41.115 287 135 8 33 301 79 349 6.06e-54 180
MS.gene50339.t1 MTR_4g118370 42.748 262 106 10 51 305 33 257 9.05e-54 176
MS.gene50339.t1 MTR_7g079400 40.339 295 133 10 33 308 67 337 1.31e-53 178
MS.gene50339.t1 MTR_4g117530 39.414 307 109 8 3 308 23 253 3.40e-51 169
MS.gene50339.t1 MTR_1g055165 39.649 285 99 8 30 308 101 318 2.40e-50 169
MS.gene50339.t1 MTR_4g114700 41.026 273 86 7 39 299 9 218 1.13e-47 160
MS.gene50339.t1 MTR_8g102520 34.897 341 161 12 3 308 8 322 1.15e-45 157
MS.gene50339.t1 MTR_1g055175 45.205 219 89 8 101 307 1 200 4.22e-45 152
MS.gene50339.t1 MTR_1g055250 36.593 317 123 12 3 308 21 270 4.83e-43 150
MS.gene50339.t1 MTR_8g013010 48.315 178 73 6 28 197 143 309 2.46e-42 148
MS.gene50339.t1 MTR_4g116340 41.860 215 67 6 3 162 36 247 2.95e-39 138
MS.gene50339.t1 MTR_2g048580 36.525 282 127 13 30 301 96 335 1.04e-36 134
MS.gene50339.t1 MTR_4g117430 49.669 151 61 6 50 197 14 152 4.06e-36 127
MS.gene50339.t1 MTR_4g122860 35.016 317 129 14 3 308 70 320 2.94e-32 122
MS.gene50339.t1 MTR_7g079420 34.602 289 119 12 38 306 905 1143 3.00e-32 127
MS.gene50339.t1 MTR_7g117720 43.503 177 48 4 3 129 10 184 1.03e-31 119
MS.gene50339.t1 MTR_5g023000 53.636 110 37 2 201 308 2 99 6.07e-28 104
MS.gene50339.t1 MTR_4g023430 59.140 93 21 2 221 308 142 222 2.91e-26 103
MS.gene50339.t1 MTR_4g118360 48.000 125 45 3 185 305 15 123 6.07e-26 100
MS.gene50339.t1 MTR_8g098830 37.640 178 86 8 109 279 35 194 7.56e-23 94.7
MS.gene50339.t1 MTR_7g079450 38.994 159 69 6 156 308 1 137 3.55e-22 90.5
MS.gene50339.t1 MTR_4g108820 30.712 267 125 13 3 228 42 289 8.19e-22 94.0
MS.gene50339.t1 MTR_2g028130 30.638 235 129 11 36 243 106 333 3.94e-21 93.2
MS.gene50339.t1 MTR_4g133170 29.412 238 134 9 32 247 86 311 1.25e-20 91.7
MS.gene50339.t1 MTR_6g034720 42.276 123 64 3 57 173 48 169 3.02e-20 87.4
MS.gene50339.t1 MTR_1g094210 25.532 282 180 9 32 308 150 406 5.18e-20 90.1
MS.gene50339.t1 MTR_1g094210 26.016 246 153 7 32 273 150 370 2.97e-19 87.8
MS.gene50339.t1 MTR_1g097560 25.088 283 162 11 32 300 127 373 3.24e-19 87.4
MS.gene50339.t1 MTR_4g023100 43.966 116 56 3 200 308 6 119 6.02e-19 81.3
MS.gene50339.t1 MTR_2g028140 31.651 218 112 11 36 223 107 317 7.79e-19 86.7
MS.gene50339.t1 MTR_1g068875 29.148 223 138 8 36 243 91 308 8.13e-19 86.3
MS.gene50339.t1 MTR_0667s0010 29.832 238 130 11 36 243 94 324 2.16e-18 85.1
MS.gene50339.t1 MTR_2g021050 25.000 368 181 14 2 304 4 341 3.20e-18 84.7
MS.gene50339.t1 MTR_1g068835 27.734 256 157 9 36 277 91 332 3.48e-18 84.3
MS.gene50339.t1 MTR_2g426020 30.736 231 132 9 36 244 100 324 6.14e-18 84.0
MS.gene50339.t1 MTR_2g024100 29.218 243 127 11 36 243 96 328 6.90e-18 83.6
MS.gene50339.t1 MTR_1g097490 26.374 273 175 8 32 300 140 390 8.79e-18 83.2
MS.gene50339.t1 MTR_4g055060 31.839 223 126 11 29 237 121 331 2.66e-17 82.0
MS.gene50339.t1 MTR_2g055970 27.365 296 178 13 16 300 291 560 2.76e-17 82.4
MS.gene50339.t1 MTR_4g070550 31.651 218 112 12 36 223 101 311 3.31e-17 81.6
MS.gene50339.t1 MTR_2g021155 31.364 220 132 10 36 244 130 341 5.46e-17 82.0
MS.gene50339.t1 MTR_2g021155 28.906 256 152 12 3 246 671 908 6.84e-16 78.6
MS.gene50339.t1 MTR_1g068860 30.435 230 129 11 36 243 91 311 1.10e-16 80.1
MS.gene50339.t1 MTR_4g107820 25.974 308 141 11 2 236 35 328 1.82e-16 79.3
MS.gene50339.t1 MTR_4g107780 25.974 308 141 11 2 236 35 328 1.82e-16 79.3
MS.gene50339.t1 MTR_2g426070 29.310 232 135 9 36 243 105 331 2.16e-16 79.3
MS.gene50339.t1 MTR_4g107820 25.974 308 141 11 2 236 35 328 2.19e-16 79.3
MS.gene50339.t1 MTR_4g107780 25.974 308 141 11 2 236 35 328 2.50e-16 79.3
MS.gene50339.t1 MTR_2g028160 29.555 247 136 12 31 244 95 336 2.60e-16 79.3
MS.gene50339.t1 MTR_3g023930 27.239 268 161 10 37 300 130 367 4.23e-16 78.2
MS.gene50339.t1 MTR_8g101580 24.865 370 188 14 2 300 44 394 4.77e-16 78.2
MS.gene50339.t1 MTR_5g097350 24.809 262 163 8 50 301 146 383 6.47e-16 77.8
MS.gene50339.t1 MTR_6g065510 27.368 285 163 12 3 261 23 289 1.05e-15 76.6
MS.gene50339.t1 MTR_2g031640 29.333 225 139 6 36 243 92 313 1.05e-15 77.4
MS.gene50339.t1 MTR_1g097330 30.049 203 127 8 33 229 106 299 1.19e-15 77.4
MS.gene50339.t1 MTR_8g013005 62.069 58 20 1 252 307 1 58 1.22e-15 70.5
MS.gene50339.t1 MTR_2g046920 29.237 236 142 10 32 254 117 340 1.26e-15 77.0
MS.gene50339.t1 MTR_8g467140 28.829 222 138 9 16 230 112 320 1.29e-15 76.6
MS.gene50339.t1 MTR_5g057790 31.429 210 123 9 36 237 142 338 1.40e-15 77.0
MS.gene50339.t1 MTR_5g086590 29.703 202 127 8 44 236 8 203 2.34e-15 74.3
MS.gene50339.t1 MTR_6g021880 28.111 217 138 6 37 242 122 331 2.69e-15 75.9
MS.gene50339.t1 MTR_2g046830 26.891 238 147 10 32 261 118 336 3.12e-15 75.9
MS.gene50339.t1 MTR_2g021150 28.326 233 145 10 34 261 113 328 3.72e-15 75.5
MS.gene50339.t1 MTR_2g425980 27.575 301 158 14 36 299 98 375 3.85e-15 75.9
MS.gene50339.t1 MTR_4g030830 25.076 331 204 12 1 300 76 393 4.88e-15 75.5
MS.gene50339.t1 MTR_8g063440 29.384 211 131 9 32 227 111 318 6.76e-15 74.7
MS.gene50339.t1 MTR_3g009310 27.039 233 145 8 49 276 157 369 6.84e-15 74.7
MS.gene50339.t1 MTR_4g117550 26.370 292 148 11 39 306 126 374 6.87e-15 74.7
MS.gene50339.t1 MTR_2g047832 25.882 255 148 10 32 261 122 360 8.75e-15 74.7
MS.gene50339.t1 MTR_7g017170 30.144 209 121 11 36 223 124 328 1.28e-14 74.3
MS.gene50339.t1 MTR_7g017130 30.144 209 121 11 36 223 124 328 1.40e-14 73.9
MS.gene50339.t1 MTR_7g070440 28.889 225 129 10 32 232 93 310 1.57e-14 73.6
MS.gene50339.t1 MTR_0129s0050 27.362 307 164 12 40 305 120 408 1.77e-14 73.9
MS.gene50339.t1 MTR_3g036290 31.282 195 107 9 34 223 117 289 1.87e-14 73.6
MS.gene50339.t1 MTR_1g055200 49.383 81 39 2 35 115 5 83 3.11e-14 68.2
MS.gene50339.t1 MTR_7g070830 28.102 274 139 13 34 261 114 375 6.76e-14 72.0
MS.gene50339.t1 MTR_7g079640 27.308 260 144 12 52 296 136 365 9.48e-14 71.6
MS.gene50339.t1 MTR_3g036430 24.432 352 183 14 3 300 20 342 1.17e-13 70.9
MS.gene50339.t1 MTR_6g079810 25.652 230 131 9 36 227 171 398 1.56e-13 71.2
MS.gene50339.t1 MTR_2g104340 25.667 300 173 10 40 307 117 398 2.09e-13 70.5
MS.gene50339.t1 MTR_3g013770 27.402 281 171 10 34 303 132 390 3.09e-13 70.1
MS.gene50339.t1 MTR_2g028110 23.567 314 163 8 2 243 3 311 3.21e-13 69.7
MS.gene50339.t1 MTR_6g065980 25.362 276 166 11 37 300 111 358 3.22e-13 69.7
MS.gene50339.t1 MTR_7g009265 27.273 308 174 15 33 302 30 325 3.48e-13 69.3
MS.gene50339.t1 MTR_1g050542 23.387 248 167 9 32 273 144 374 4.64e-13 69.3
MS.gene50339.t1 MTR_7g070880 27.372 274 141 13 34 261 114 375 5.57e-13 69.3
MS.gene50339.t1 MTR_4g028510 27.982 218 123 9 36 223 120 333 5.67e-13 69.3
MS.gene50339.t1 MTR_5g064700 22.837 289 170 10 52 305 98 368 7.58e-13 68.6
MS.gene50339.t1 MTR_0129s0070 25.758 264 155 9 40 280 124 369 7.80e-13 68.9
MS.gene50339.t1 MTR_5g069120 42.105 95 45 2 33 117 83 177 8.58e-13 65.9
MS.gene50339.t1 MTR_7g069550 28.727 275 142 14 36 280 123 373 1.06e-12 68.6
MS.gene50339.t1 MTR_7g445890 23.780 328 177 11 3 261 16 339 1.14e-12 68.2
MS.gene50339.t1 MTR_5g022420 27.778 252 144 14 32 273 118 341 1.25e-12 68.2
MS.gene50339.t1 MTR_5g072060 29.740 269 134 15 35 280 109 345 1.80e-12 68.2
MS.gene50339.t1 MTR_7g066850 24.667 300 184 12 34 303 120 407 2.31e-12 67.4
MS.gene50339.t1 MTR_3g007360 28.070 285 160 16 36 301 145 403 3.25e-12 67.0
MS.gene50339.t1 MTR_7g034310 27.572 243 129 11 77 302 17 229 3.26e-12 65.5
MS.gene50339.t1 MTR_0054s0100 26.230 244 142 8 13 223 101 339 3.37e-12 67.0
MS.gene50339.t1 MTR_8g064890 27.941 204 118 9 40 223 125 319 3.43e-12 67.0
MS.gene50339.t1 MTR_1g053510 27.004 237 126 10 30 227 113 341 3.60e-12 67.0
MS.gene50339.t1 MTR_7g062060 25.763 295 177 13 33 302 124 401 4.23e-12 66.6
MS.gene50339.t1 MTR_3g013740 27.076 277 171 12 36 300 149 406 5.16e-12 66.2
MS.gene50339.t1 MTR_2g078870 26.148 283 168 16 31 300 53 307 6.57e-12 65.5
MS.gene50339.t1 MTR_3g010920 26.000 300 180 13 7 300 118 381 8.44e-12 65.5
MS.gene50339.t1 MTR_3g083660 26.549 226 127 9 3 228 25 211 1.11e-11 64.7
MS.gene50339.t1 MTR_7g055830 26.171 363 190 17 3 303 13 359 1.20e-11 65.1
MS.gene50339.t1 MTR_8g039500 27.404 208 134 7 30 223 104 308 1.22e-11 65.1
MS.gene50339.t1 MTR_2g021190 26.978 278 171 13 34 300 116 372 1.24e-11 65.1
MS.gene50339.t1 MTR_3g009300 26.154 260 138 10 6 223 37 284 1.26e-11 65.1
MS.gene50339.t1 MTR_4g028750 27.354 223 114 8 36 223 120 329 1.27e-11 65.1
MS.gene50339.t1 MTR_2g046900 27.049 244 147 12 32 261 121 347 1.36e-11 65.1
MS.gene50339.t1 MTR_7g078900 25.086 291 172 10 33 295 122 394 1.58e-11 65.1
MS.gene50339.t1 MTR_8g063370 26.455 189 126 3 44 222 134 319 1.76e-11 64.7
MS.gene50339.t1 MTR_2g021800 25.316 316 174 13 30 301 115 412 1.80e-11 64.7
MS.gene50339.t1 MTR_6g017305 27.411 197 126 7 40 223 5 197 1.84e-11 63.9
MS.gene50339.t1 MTR_5g011200 27.586 203 124 8 36 228 117 306 1.91e-11 64.7
MS.gene50339.t1 MTR_0760s0010 26.277 274 159 12 37 300 149 389 1.95e-11 64.7
MS.gene50339.t1 MTR_3g007330 23.948 309 192 13 11 300 108 392 2.08e-11 64.3
MS.gene50339.t1 MTR_1004s0010 26.277 274 159 12 37 300 119 359 2.21e-11 64.3
MS.gene50339.t1 MTR_4g028680 27.273 220 128 9 36 227 120 335 2.24e-11 64.3
MS.gene50339.t1 MTR_3g011060 26.449 276 163 12 34 299 140 385 2.27e-11 64.3
MS.gene50339.t1 MTR_7g057270 26.538 260 147 11 34 261 123 370 2.52e-11 64.3
MS.gene50339.t1 MTR_7g017040 25.490 306 178 16 32 306 113 399 2.77e-11 64.3
MS.gene50339.t1 MTR_2g066170 26.415 212 140 6 27 223 107 317 3.05e-11 63.9
MS.gene50339.t1 MTR_4g013180 25.215 349 160 12 3 261 11 348 3.30e-11 63.9
MS.gene50339.t1 MTR_1g112010 23.482 247 155 8 34 273 76 295 3.81e-11 63.5
MS.gene50339.t1 MTR_4g019990 24.046 262 164 9 22 261 96 344 4.16e-11 63.5
MS.gene50339.t1 MTR_4g013175 25.564 266 145 7 34 261 121 371 4.38e-11 63.5
MS.gene50339.t1 MTR_3g010390 25.234 214 138 8 36 234 136 342 5.18e-11 63.2
MS.gene50339.t1 MTR_3g006570 26.259 278 172 11 37 300 118 376 5.44e-11 63.2
MS.gene50339.t1 MTR_4g024890 23.954 263 171 9 32 275 105 357 5.94e-11 63.2
MS.gene50339.t1 MTR_4g028660 25.701 214 131 7 36 223 119 330 6.18e-11 63.2
MS.gene50339.t1 MTR_4g027450 25.701 214 131 7 36 223 119 330 6.18e-11 63.2
MS.gene50339.t1 MTR_1g055065 26.941 219 132 10 28 229 13 220 6.57e-11 62.4
MS.gene50339.t1 MTR_6g016285 25.157 318 187 12 32 304 113 424 6.63e-11 63.2
MS.gene50339.t1 MTR_7g066840 26.263 297 174 13 34 303 122 400 7.91e-11 62.8
MS.gene50339.t1 MTR_3g014570 25.965 285 168 12 36 303 123 381 8.72e-11 62.4
MS.gene50339.t1 MTR_5g056020 25.868 317 178 13 34 300 111 420 8.96e-11 62.8
MS.gene50339.t1 MTR_4g130330 24.585 301 177 12 36 303 106 389 9.21e-11 62.4
MS.gene50339.t1 MTR_3g006500 24.904 261 150 12 51 300 144 369 9.38e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50339.t1 AT4G12560 26.367 311 176 13 31 308 88 378 2.14e-18 85.5
MS.gene50339.t1 AT4G12560 26.367 311 176 13 31 308 88 378 2.14e-18 85.5
MS.gene50339.t1 AT4G12560 26.367 311 176 13 31 308 88 378 2.14e-18 85.5
MS.gene50339.t1 AT3G06240 26.821 302 180 9 10 300 148 419 4.54e-17 81.6
MS.gene50339.t1 AT4G22390 27.039 233 145 10 33 244 90 318 8.29e-12 65.9
MS.gene50339.t1 AT4G22390 27.039 233 145 10 33 244 90 318 9.20e-12 65.5
MS.gene50339.t1 AT1G32600 28.873 142 82 6 37 175 47 172 1.62e-11 64.3

Find 68 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGAATGGATTAACTTTAT+TGG 0.141488 4.4:+78365057 None:intergenic
ACAGAAAATGGTGATATTAT+TGG 0.164193 4.4:-78364575 MS.gene50339:CDS
GAAGCGTATATTAATCTATA+TGG 0.223056 4.4:-78365127 MS.gene50339:CDS
CATTCTGGTTTGTGGGTATC+TGG 0.231608 4.4:-78364785 MS.gene50339:CDS
GTAATGATACAATTGAAATA+TGG 0.247553 4.4:-78364730 MS.gene50339:CDS
CTGGAGAATTTCTCAGTCTA+TGG 0.261173 4.4:-78364766 MS.gene50339:CDS
CCTATGTATTCTACAGAAAA+TGG 0.272991 4.4:-78364587 MS.gene50339:CDS
CTTCCTCAGCATGTCATTCC+TGG 0.289129 4.4:-78364659 MS.gene50339:CDS
CATTGTTGGCTTTGATTTAA+CGG 0.305680 4.4:-78364864 MS.gene50339:CDS
TCAAATTCTGTTGAGGTTAC+CGG 0.316216 4.4:-78365497 MS.gene50339:CDS
CACAGAGCATTAGTACATTC+TGG 0.320173 4.4:-78364800 MS.gene50339:CDS
GTGAAAGTAGTCTGCAAATC+TGG 0.335248 4.4:+78364610 None:intergenic
TTCGCGTCGTGATTTAGTTT+TGG 0.338685 4.4:-78364891 MS.gene50339:CDS
GTCATCCAAACATTTACATT+TGG 0.361833 4.4:-78365201 MS.gene50339:CDS
GAGGGTACTCACTTACCTTA+TGG 0.383661 4.4:-78364971 MS.gene50339:CDS
TATATTAATCTATATGGGTT+TGG 0.387173 4.4:-78365121 MS.gene50339:CDS
AGTCTATGGGTTAAGGATAC+TGG 0.398340 4.4:-78364752 MS.gene50339:CDS
GGATTCCAAATGTAAATGTT+TGG 0.401055 4.4:+78365196 None:intergenic
TGGTGACAATGAGCATGTTT+AGG 0.402786 4.4:-78364432 None:intergenic
ATTTCTCAGTCTATGGGTTA+AGG 0.409114 4.4:-78364759 MS.gene50339:CDS
GCTCTGTGATCAACATCATT+TGG 0.411538 4.4:+78364815 None:intergenic
AAAGTTAATCCATTCTTAGT+TGG 0.417793 4.4:-78365052 MS.gene50339:CDS
TAATGATACAATTGAAATAT+GGG 0.423786 4.4:-78364729 MS.gene50339:CDS
ATTAGTACATTCTGGTTTGT+GGG 0.428082 4.4:-78364792 MS.gene50339:CDS
CATTAGTACATTCTGGTTTG+TGG 0.428815 4.4:-78364793 MS.gene50339:CDS
TGGTGTTCCTCAATCACGTT+TGG 0.430084 4.4:-78365032 MS.gene50339:CDS
AAGCGTATATTAATCTATAT+GGG 0.430207 4.4:-78365126 MS.gene50339:CDS
GGTGATATTATTGGAAAATA+CGG 0.431474 4.4:-78364566 MS.gene50339:CDS
ACATGACCCTTTAATTTCAA+AGG 0.452023 4.4:+78365246 None:intergenic
CATGACCCTTTAATTTCAAA+GGG 0.458275 4.4:+78365247 None:intergenic
CTCGGTATACACGACTCCTT+CGG 0.477674 4.4:+78364472 None:intergenic
TGATCAACATCATTTGGAAC+AGG 0.489346 4.4:+78364821 None:intergenic
TCTCTATAATCGTCGCCATA+AGG 0.490230 4.4:+78364956 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.494278 4.4:-78364697 MS.gene50339:CDS
TGAGGAACACCAACTAAGAA+TGG 0.498401 4.4:+78365043 None:intergenic
AAAATACGGTGGTACTAGAT+TGG 0.512046 4.4:-78364552 MS.gene50339:CDS
ATATAGATTAATATACGCTT+CGG 0.513373 4.4:+78365129 None:intergenic
TGGAGAATTTCTCAGTCTAT+GGG 0.524018 4.4:-78364765 MS.gene50339:CDS
TACATTTGGAATCCATCCAC+CGG 0.532440 4.4:-78365187 MS.gene50339:CDS
GAAATTAAAGGGTCATGTAG+AGG 0.538383 4.4:-78365241 MS.gene50339:CDS
TCAAAGCATGGATCTCCCTC+AGG 0.540191 4.4:-78365530 None:intergenic
GAGTCGCTACTTTCACTCTC+TGG 0.542238 4.4:-78364452 MS.gene50339:CDS
TGATCATTCAAATTCTGTTG+AGG 0.546882 4.4:-78365504 MS.gene50339:CDS
AAATTAAAGGGTCATGTAGA+GGG 0.550758 4.4:-78365240 MS.gene50339:CDS
TTCGGATGGACTGTTGCAGA+AGG 0.564578 4.4:+78364490 None:intergenic
ACGATTATAGAGAAGGAGAA+GGG 0.565105 4.4:-78364946 MS.gene50339:CDS
TTATAGAGAAGGAGAAGGGG+TGG 0.585026 4.4:-78364942 MS.gene50339:CDS
TTGGCTTTGATTTAACGGAA+AGG 0.585773 4.4:-78364859 MS.gene50339:CDS
GACGATTATAGAGAAGGAGA+AGG 0.587454 4.4:-78364947 MS.gene50339:CDS
CAAATCTGGAATAGTACAGA+GGG 0.587792 4.4:+78364624 None:intergenic
TTCTGCAACAGTCCATCCGA+AGG 0.595731 4.4:-78364488 MS.gene50339:CDS
TATGGCGACGATTATAGAGA+AGG 0.601035 4.4:-78364953 MS.gene50339:CDS
TCGGAATTAAAAGCAGACAT+TGG 0.601452 4.4:+78365148 None:intergenic
GTCTAGAGATGATTATGTGG+TGG 0.603968 4.4:-78365089 MS.gene50339:CDS
GCAAATCTGGAATAGTACAG+AGG 0.613631 4.4:+78364623 None:intergenic
TTTGAATGATCAGTTCCTGA+GGG 0.621173 4.4:+78365515 None:intergenic
CGATTATAGAGAAGGAGAAG+GGG 0.628339 4.4:-78364945 MS.gene50339:CDS
ATTTGAATGATCAGTTCCTG+AGG 0.632781 4.4:+78365514 None:intergenic
TGACCAGGAATGACATGCTG+AGG 0.635899 4.4:+78364656 None:intergenic
GTATACACGACTCCTTCGGA+TGG 0.638472 4.4:+78364476 None:intergenic
TTGGTGAAGTATAATGACAA+AGG 0.639076 4.4:-78364533 MS.gene50339:CDS
CAATGCGTGGAAAGAAATCG+AGG 0.642429 4.4:-78364990 MS.gene50339:CDS
AGAGGGTTAAAGTAGTGACC+AGG 0.655673 4.4:+78364641 None:intergenic
AGAGAGTGAAAGTAGCGACT+CGG 0.677615 4.4:+78364454 None:intergenic
TCTCGTTTAGAGACAATGCG+TGG 0.682425 4.4:-78365003 MS.gene50339:CDS
TCAGTCTAGAGATGATTATG+TGG 0.684477 4.4:-78365092 MS.gene50339:CDS
GATATTATTGGAAAATACGG+TGG 0.691433 4.4:-78364563 MS.gene50339:CDS
AATGCGTGGAAAGAAATCGA+GGG 0.697045 4.4:-78364989 MS.gene50339:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGATACAATTGAAATAT+GGG - chr4.4:78365227-78365246 MS.gene50339:CDS 15.0%
!! AAGCGTATATTAATCTATAT+GGG - chr4.4:78364830-78364849 MS.gene50339:CDS 20.0%
!! ATATAGATTAATATACGCTT+CGG + chr4.4:78364830-78364849 None:intergenic 20.0%
!! GTAATGATACAATTGAAATA+TGG - chr4.4:78365226-78365245 MS.gene50339:CDS 20.0%
!! TATATTAATCTATATGGGTT+TGG - chr4.4:78364835-78364854 MS.gene50339:CDS 20.0%
!!! ATTTTCCCTTTGAAATTAAA+GGG - chr4.4:78364704-78364723 MS.gene50339:CDS 20.0%
!!! TAAGAATGGATTAACTTTAT+TGG + chr4.4:78364902-78364921 None:intergenic 20.0%
!!! TATTTTCCCTTTGAAATTAA+AGG - chr4.4:78364703-78364722 MS.gene50339:CDS 20.0%
! AAAAATATAAGAGACTTCAC+CGG + chr4.4:78364481-78364500 None:intergenic 25.0%
! AAAGTTAATCCATTCTTAGT+TGG - chr4.4:78364904-78364923 MS.gene50339:CDS 25.0%
! ACAGAAAATGGTGATATTAT+TGG - chr4.4:78365381-78365400 MS.gene50339:intron 25.0%
! GAAGCGTATATTAATCTATA+TGG - chr4.4:78364829-78364848 MS.gene50339:CDS 25.0%
! GGTGATATTATTGGAAAATA+CGG - chr4.4:78365390-78365409 MS.gene50339:intron 25.0%
!! AAATGTGAATTTTCAAAGTG+AGG + chr4.4:78364544-78364563 None:intergenic 25.0%
!! CATTTTCTGTAGAATACATA+GGG + chr4.4:78365371-78365390 None:intergenic 25.0%
AAATTAAAGGGTCATGTAGA+GGG - chr4.4:78364716-78364735 MS.gene50339:CDS 30.0%
ATCTCATGAAATTCGATCTA+TGG - chr4.4:78364615-78364634 MS.gene50339:CDS 30.0%
CCTATGTATTCTACAGAAAA+TGG - chr4.4:78365369-78365388 MS.gene50339:intron 30.0%
GATATTATTGGAAAATACGG+TGG - chr4.4:78365393-78365412 MS.gene50339:intron 30.0%
GGATTCCAAATGTAAATGTT+TGG + chr4.4:78364763-78364782 None:intergenic 30.0%
GTCATCCAAACATTTACATT+TGG - chr4.4:78364755-78364774 MS.gene50339:CDS 30.0%
TCAAATGCGTTTGTAAGTTA+TGG - chr4.4:78364500-78364519 MS.gene50339:CDS 30.0%
TGATCATTCAAATTCTGTTG+AGG - chr4.4:78364452-78364471 MS.gene50339:CDS 30.0%
TTGGTGAAGTATAATGACAA+AGG - chr4.4:78365423-78365442 MS.gene50339:intron 30.0%
! ACATGACCCTTTAATTTCAA+AGG + chr4.4:78364713-78364732 None:intergenic 30.0%
! CATGACCCTTTAATTTCAAA+GGG + chr4.4:78364712-78364731 None:intergenic 30.0%
! CCATTTTCTGTAGAATACAT+AGG + chr4.4:78365372-78365391 None:intergenic 30.0%
! TTTTTAATGGGGCTATTCAT+TGG - chr4.4:78365037-78365056 MS.gene50339:CDS 30.0%
!! ATTAGTACATTCTGGTTTGT+GGG - chr4.4:78365164-78365183 MS.gene50339:CDS 30.0%
!! CATTGTTGGCTTTGATTTAA+CGG - chr4.4:78365092-78365111 MS.gene50339:CDS 30.0%
!!! TTAGTTTTGGATGTCATTGT+TGG - chr4.4:78365078-78365097 MS.gene50339:CDS 30.0%
AAAAACTCCAAACGTGATTG+AGG + chr4.4:78364934-78364953 None:intergenic 35.0%
AAAATACGGTGGTACTAGAT+TGG - chr4.4:78365404-78365423 MS.gene50339:intron 35.0%
AATACAAAGTGCATTCGTCT+TGG - chr4.4:78365259-78365278 MS.gene50339:CDS 35.0%
ACGATTATAGAGAAGGAGAA+GGG - chr4.4:78365010-78365029 MS.gene50339:CDS 35.0%
ATTTGAATGATCAGTTCCTG+AGG + chr4.4:78364445-78364464 None:intergenic 35.0%
GAAATTAAAGGGTCATGTAG+AGG - chr4.4:78364715-78364734 MS.gene50339:CDS 35.0%
TCAAAGGGAAAATAAGAGTC+TGG + chr4.4:78364697-78364716 None:intergenic 35.0%
TCAAATTCTGTTGAGGTTAC+CGG - chr4.4:78364459-78364478 MS.gene50339:CDS 35.0%
TCAGTCTAGAGATGATTATG+TGG - chr4.4:78364864-78364883 MS.gene50339:CDS 35.0%
TCGGAATTAAAAGCAGACAT+TGG + chr4.4:78364811-78364830 None:intergenic 35.0%
TGGAGAATTTCTCAGTCTAT+GGG - chr4.4:78365191-78365210 MS.gene50339:CDS 35.0%
TTTGAATGATCAGTTCCTGA+GGG + chr4.4:78364444-78364463 None:intergenic 35.0%
! ATTTCTCAGTCTATGGGTTA+AGG - chr4.4:78365197-78365216 MS.gene50339:CDS 35.0%
! CAAATCTGGAATAGTACAGA+GGG + chr4.4:78365335-78365354 None:intergenic 35.0%
! TGATCAACATCATTTGGAAC+AGG + chr4.4:78365138-78365157 None:intergenic 35.0%
! TGGTATTTGTCTTTTCGATC+CGG + chr4.4:78364791-78364810 None:intergenic 35.0%
!! CATTAGTACATTCTGGTTTG+TGG - chr4.4:78365163-78365182 MS.gene50339:CDS 35.0%
!! TTGGCTTTGATTTAACGGAA+AGG - chr4.4:78365097-78365116 MS.gene50339:CDS 35.0%
AATGCGTGGAAAGAAATCGA+GGG - chr4.4:78364967-78364986 MS.gene50339:CDS 40.0%
AGGGAAAATAAGAGTCTGGA+AGG + chr4.4:78364693-78364712 None:intergenic 40.0%
ATCTACTAGTGTGTGTTGCA+AGG + chr4.4:78364576-78364595 None:intergenic 40.0%
CGATTATAGAGAAGGAGAAG+GGG - chr4.4:78365011-78365030 MS.gene50339:CDS 40.0%
CTGGAGAATTTCTCAGTCTA+TGG - chr4.4:78365190-78365209 MS.gene50339:CDS 40.0%
GACGATTATAGAGAAGGAGA+AGG - chr4.4:78365009-78365028 MS.gene50339:CDS 40.0%
GCTCTGTGATCAACATCATT+TGG + chr4.4:78365144-78365163 None:intergenic 40.0%
GGAAGGGAACAGTTAAAGTT+GGG + chr4.4:78364676-78364695 None:intergenic 40.0%
GGGAAAATAAGAGTCTGGAA+GGG + chr4.4:78364692-78364711 None:intergenic 40.0%
GTCTAGAGATGATTATGTGG+TGG - chr4.4:78364867-78364886 MS.gene50339:CDS 40.0%
GTGAAAGTAGTCTGCAAATC+TGG + chr4.4:78365349-78365368 None:intergenic 40.0%
TATGGCGACGATTATAGAGA+AGG - chr4.4:78365003-78365022 MS.gene50339:CDS 40.0%
TCTCTATAATCGTCGCCATA+AGG + chr4.4:78365003-78365022 None:intergenic 40.0%
TGAGGAACACCAACTAAGAA+TGG + chr4.4:78364916-78364935 None:intergenic 40.0%
TGGAAGGGAACAGTTAAAGT+TGG + chr4.4:78364677-78364696 None:intergenic 40.0%
TGTGTGTTGCAAGGTTAAGT+TGG + chr4.4:78364567-78364586 None:intergenic 40.0%
! AGAAGGGGTGGTTTTTAATG+GGG - chr4.4:78365026-78365045 MS.gene50339:CDS 40.0%
! AGTCTATGGGTTAAGGATAC+TGG - chr4.4:78365204-78365223 MS.gene50339:CDS 40.0%
! CACAGAGCATTAGTACATTC+TGG - chr4.4:78365156-78365175 MS.gene50339:CDS 40.0%
! GAGAAGGGGTGGTTTTTAAT+GGG - chr4.4:78365025-78365044 MS.gene50339:CDS 40.0%
! GCAAATCTGGAATAGTACAG+AGG + chr4.4:78365336-78365355 None:intergenic 40.0%
! TACATTTGGAATCCATCCAC+CGG - chr4.4:78364769-78364788 MS.gene50339:CDS 40.0%
! TATTTGTCTTTTCGATCCGG+TGG + chr4.4:78364788-78364807 None:intergenic 40.0%
!! TTCGCGTCGTGATTTAGTTT+TGG - chr4.4:78365065-78365084 MS.gene50339:CDS 40.0%
AGAGAGTGAAAGTAGCGACT+CGG + chr4.4:78365505-78365524 None:intergenic 45.0%
AGAGGGTTAAAGTAGTGACC+AGG + chr4.4:78365318-78365337 None:intergenic 45.0%
CAATGCGTGGAAAGAAATCG+AGG - chr4.4:78364966-78364985 MS.gene50339:CDS 45.0%
CAGTTAAAGTTGGGTGAAGC+AGG + chr4.4:78364667-78364686 None:intergenic 45.0%
TCTCGTTTAGAGACAATGCG+TGG - chr4.4:78364953-78364972 MS.gene50339:CDS 45.0%
TGGTGTTCCTCAATCACGTT+TGG - chr4.4:78364924-78364943 MS.gene50339:CDS 45.0%
TTATAGAGAAGGAGAAGGGG+TGG - chr4.4:78365014-78365033 MS.gene50339:CDS 45.0%
! GGAGAAGGGGTGGTTTTTAA+TGG - chr4.4:78365024-78365043 MS.gene50339:CDS 45.0%
!! CATTCTGGTTTGTGGGTATC+TGG - chr4.4:78365171-78365190 MS.gene50339:CDS 45.0%
!! GAGGGTACTCACTTACCTTA+TGG - chr4.4:78364985-78365004 MS.gene50339:CDS 45.0%
CTCGGTATACACGACTCCTT+CGG + chr4.4:78365487-78365506 None:intergenic 50.0%
CTTCCTCAGCATGTCATTCC+TGG - chr4.4:78365297-78365316 MS.gene50339:intron 50.0%
GTATACACGACTCCTTCGGA+TGG + chr4.4:78365483-78365502 None:intergenic 50.0%
TGACCAGGAATGACATGCTG+AGG + chr4.4:78365303-78365322 None:intergenic 50.0%
TTCTGCAACAGTCCATCCGA+AGG - chr4.4:78365468-78365487 MS.gene50339:CDS 50.0%
! GAGTCGCTACTTTCACTCTC+TGG - chr4.4:78365504-78365523 MS.gene50339:CDS 50.0%
! TGTCTTTTCGATCCGGTGGA+TGG + chr4.4:78364784-78364803 None:intergenic 50.0%
!! TTCGGATGGACTGTTGCAGA+AGG + chr4.4:78365469-78365488 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 78364433 78365545 78364433 ID=MS.gene50339
chr4.4 mRNA 78364433 78365545 78364433 ID=MS.gene50339.t1;Parent=MS.gene50339
chr4.4 exon 78365467 78365545 78365467 ID=MS.gene50339.t1.exon1;Parent=MS.gene50339.t1
chr4.4 CDS 78365467 78365545 78365467 ID=cds.MS.gene50339.t1;Parent=MS.gene50339.t1
chr4.4 exon 78364433 78365280 78364433 ID=MS.gene50339.t1.exon2;Parent=MS.gene50339.t1
chr4.4 CDS 78364433 78365280 78364433 ID=cds.MS.gene50339.t1;Parent=MS.gene50339.t1
Gene Sequence

>MS.gene50339

ATGGATCTCCCTCAGGAACTGATCATTCAAATTCTGTTGAGGTTACCGGTGAAGTCTCTTATATTTTTCAAATGCGTTTGTAAGTTATGGTTTTCTATTATCTCTGATCCTCACTTTGAAAATTCACATTTCCAACTTAACCTTGCAACACACACTAGTAGATTTTTATGCATTTCAGCTCTATCTCATGAAATTCGATCTATGGATTTTGATGCATTTCTTAATGATTCTCCTGCTTCACCCAACTTTAACTGTTCCCTTCCAGACTCTTATTTTCCCTTTGAAATTAAAGGGTCATGTAGAGGGTTTATATTCTTGTATCGTCATCCAAACATTTACATTTGGAATCCATCCACCGGATCGAAAAGACAAATACCAATGTCTGCTTTTAATTCCGAAGCGTATATTAATCTATATGGGTTTGGTTATGATCAGTCTAGAGATGATTATGTGGTGGTTTTACTGTCCAATAAAGTTAATCCATTCTTAGTTGGTGTTCCTCAATCACGTTTGGAGTTTTTCTCGTTTAGAGACAATGCGTGGAAAGAAATCGAGGGTACTCACTTACCTTATGGCGACGATTATAGAGAAGGAGAAGGGGTGGTTTTTAATGGGGCTATTCATTGGTTGTCTTCGCGTCGTGATTTAGTTTTGGATGTCATTGTTGGCTTTGATTTAACGGAAAGGAAATTGTTTGAGATGCCTGTTCCAAATGATGTTGATCACAGAGCATTAGTACATTCTGGTTTGTGGGTATCTGGAGAATTTCTCAGTCTATGGGTTAAGGATACTGGTAATGATACAATTGAAATATGGGTTATGAAAGAATACAAAGTGCATTCGTCTTGGACTAAGAGTCTTGTTCTTCCTCAGCATGTCATTCCTGGTCACTACTTTAACCCTCTGTACTATTCCAGATTTGCAGACTACTTTCACCCTATGTATTCTACAGAAAATGGTGATATTATTGGAAAATACGGTGGTACTAGATTGGTGAAGTATAATGACAAAGGACAGTTTTTAGAGCATCACTCCTTCTGCAACAGTCCATCCGAAGGAGTCGTGTATACCGAGTCGCTACTTTCACTCTCTGGTGACAATGAGCATGTTTAG
Protein sequence

>MS.gene50339.t1

MDLPQELIIQILLRLPVKSLIFFKCVYSYFPFEIKGSCRGFIFLYRHPNIYIWNPSTGSKRQIPMSAFNSEAYINLYGFGYDQSRDDYVVVLLSNKVNPFLVGVPQSRLEFFSFRDNAWKEIEGTHLPYGDDYREGEGVVFNGAIHWLSSRRDLVLDVIVGFDLTERKLFEMPVPNDVDHRALVHSGLWVSGEFLSLWVKDTGNDTIEIWVMKEYKVHSSWTKSLVLPQHVIPGHYFNPLYYSRFADYFHPMYSTENGDIIGKYGGTRLVKYNDKGQFLEHHSFCNSPSEGVVYTESLLSLSGDNEHV