AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50301


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50301.t1 MTR_4g023080 73.973 146 38 0 1 146 208 353 1.59e-72 221
MS.gene50301.t1 MTR_4g023130 72.603 146 40 0 1 146 167 312 6.48e-71 216
MS.gene50301.t1 MTR_4g023090 68.293 164 35 3 1 147 223 386 1.08e-70 217
MS.gene50301.t1 MTR_4g023380 65.698 172 34 3 1 147 208 379 3.02e-69 213
MS.gene50301.t1 MTR_4g021785 68.027 147 47 0 1 147 209 355 1.44e-66 206
MS.gene50301.t1 MTR_4g023070 61.875 160 47 2 1 146 210 369 1.95e-60 191
MS.gene50301.t1 MTR_4g023120 60.870 161 49 2 1 147 210 370 1.28e-59 188
MS.gene50301.t1 MTR_3g103020 62.667 150 53 2 1 147 229 378 2.65e-58 185
MS.gene50301.t1 MTR_8g040935 60.390 154 54 3 1 147 55 208 9.50e-57 176
MS.gene50301.t1 MTR_4g109270 61.333 150 55 2 1 147 215 364 2.14e-56 180
MS.gene50301.t1 MTR_4g023440 86.458 96 13 0 52 147 237 332 2.57e-53 171
MS.gene50301.t1 MTR_4g023150 62.667 150 51 3 1 145 209 358 5.46e-53 171
MS.gene50301.t1 MTR_4g116330 57.237 152 60 2 1 147 229 380 1.54e-52 171
MS.gene50301.t1 MTR_4g023190 62.000 150 52 3 1 145 208 357 2.65e-52 169
MS.gene50301.t1 MTR_4g023200 61.333 150 53 3 1 145 209 358 2.89e-51 167
MS.gene50301.t1 MTR_6g060650 56.376 149 60 3 1 146 226 372 2.39e-50 165
MS.gene50301.t1 MTR_4g116320 59.060 149 54 3 1 144 109 255 1.36e-49 159
MS.gene50301.t1 MTR_8g433040 60.145 138 53 2 11 147 228 364 2.67e-47 157
MS.gene50301.t1 MTR_8g032380 60.145 138 53 2 11 147 228 364 2.67e-47 157
MS.gene50301.t1 MTR_4g109280 55.921 152 59 4 1 147 211 359 3.36e-47 156
MS.gene50301.t1 MTR_4g117780 58.170 153 58 2 1 147 215 367 8.13e-47 155
MS.gene50301.t1 MTR_6g463430 59.603 151 52 4 1 147 57 202 3.47e-46 149
MS.gene50301.t1 MTR_3g034180 57.047 149 58 4 2 147 344 489 6.35e-46 155
MS.gene50301.t1 MTR_4g117770 58.170 153 58 2 1 147 215 367 7.93e-46 153
MS.gene50301.t1 MTR_4g117810 58.170 153 58 2 1 147 215 367 7.93e-46 153
MS.gene50301.t1 MTR_4g117720 56.051 157 57 5 1 147 224 378 1.90e-45 152
MS.gene50301.t1 MTR_4g117850 56.863 153 54 6 1 144 227 376 2.19e-45 152
MS.gene50301.t1 MTR_4g117870 60.465 129 46 4 22 147 141 267 1.40e-44 147
MS.gene50301.t1 MTR_8g433030 59.259 135 50 4 11 142 205 337 4.40e-44 147
MS.gene50301.t1 MTR_8g032390 59.259 135 50 4 11 142 238 370 9.56e-44 147
MS.gene50301.t1 MTR_4g122850 56.579 152 59 6 1 147 215 364 3.03e-43 146
MS.gene50301.t1 MTR_5g097210 57.143 147 55 5 1 143 217 359 2.28e-42 144
MS.gene50301.t1 MTR_3g034160 57.823 147 58 3 2 146 230 374 2.56e-42 144
MS.gene50301.t1 MTR_5g070130 50.000 152 71 3 1 147 226 377 5.32e-42 143
MS.gene50301.t1 MTR_4g094365 54.248 153 62 5 1 147 214 364 7.50e-42 142
MS.gene50301.t1 MTR_5g070080 55.629 151 62 4 1 147 237 386 8.79e-41 140
MS.gene50301.t1 MTR_5g070088 50.968 155 65 4 1 147 235 386 8.56e-40 137
MS.gene50301.t1 MTR_1g055195 51.007 149 64 5 1 144 331 475 3.22e-39 138
MS.gene50301.t1 MTR_4g023430 79.012 81 12 1 72 147 142 222 3.45e-39 132
MS.gene50301.t1 MTR_3g034130 52.632 133 60 2 1 132 229 359 1.34e-38 134
MS.gene50301.t1 MTR_1g055175 46.667 150 74 4 1 146 53 200 3.22e-37 126
MS.gene50301.t1 MTR_5g023000 61.616 99 36 1 51 147 1 99 1.93e-36 121
MS.gene50301.t1 MTR_4g118360 55.039 129 47 5 25 146 1 125 2.52e-36 121
MS.gene50301.t1 MTR_4g118380 53.030 132 50 3 1 122 228 357 6.24e-36 127
MS.gene50301.t1 MTR_1g055165 46.358 151 75 4 1 147 170 318 1.06e-35 125
MS.gene50301.t1 MTR_1g055135 45.806 155 73 4 1 147 224 375 3.03e-35 125
MS.gene50301.t1 MTR_4g118400 51.748 143 54 4 1 141 165 294 5.78e-35 123
MS.gene50301.t1 MTR_4g116330 57.658 111 43 1 41 147 231 341 1.97e-34 122
MS.gene50301.t1 MTR_4g117750 50.758 132 54 2 25 145 350 481 8.15e-33 120
MS.gene50301.t1 MTR_4g117750 47.436 156 68 4 1 146 207 358 2.69e-28 108
MS.gene50301.t1 MTR_4g118370 50.388 129 57 4 19 144 133 257 3.02e-31 112
MS.gene50301.t1 MTR_4g117530 42.857 147 54 3 1 147 137 253 1.09e-30 111
MS.gene50301.t1 MTR_1g055250 42.963 135 72 4 1 134 123 253 2.45e-29 109
MS.gene50301.t1 MTR_7g079450 43.262 141 72 4 10 147 2 137 7.75e-29 103
MS.gene50301.t1 MTR_7g079400 44.595 148 68 5 10 147 194 337 7.85e-29 108
MS.gene50301.t1 MTR_7g079370 48.175 137 62 4 10 141 218 350 8.15e-29 108
MS.gene50301.t1 MTR_4g023100 83.636 55 9 0 93 147 65 119 5.96e-26 95.1
MS.gene50301.t1 MTR_8g102520 40.278 144 76 4 10 147 183 322 6.83e-26 100
MS.gene50301.t1 MTR_4g122860 48.438 128 56 5 27 147 196 320 7.63e-26 100
MS.gene50301.t1 MTR_7g117720 61.628 86 27 2 62 141 186 271 9.81e-25 96.3
MS.gene50301.t1 MTR_4g121580 48.739 119 50 4 21 137 230 339 1.30e-23 94.7
MS.gene50301.t1 MTR_4g122840 44.118 136 37 5 16 147 164 264 9.22e-22 88.2
MS.gene50301.t1 MTR_7g079420 39.073 151 77 6 6 147 1000 1144 4.37e-21 88.6
MS.gene50301.t1 MTR_8g098830 40.000 135 71 5 10 141 88 215 5.02e-21 85.5
MS.gene50301.t1 MTR_4g114700 50.515 97 41 2 49 138 122 218 2.58e-20 84.0
MS.gene50301.t1 MTR_2g048580 36.301 146 71 6 10 147 211 342 1.80e-18 80.5
MS.gene50301.t1 MTR_8g013005 62.712 59 20 1 91 147 1 59 1.36e-16 69.7
MS.gene50301.t1 MTR_2g064960 67.442 43 12 1 107 147 17 59 4.18e-12 58.2
MS.gene50301.t1 MTR_4g109290 62.264 53 18 1 97 147 136 188 7.94e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAACCATTTACTTAATTC+TGG 0.247939 4.4:-77642691 MS.gene50301:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.267319 4.4:-77642657 MS.gene50301:CDS
ATGATAAAGGACAGCTATTA+AGG 0.278962 4.4:-77642447 MS.gene50301:CDS
ATAAGGATACAGTTGAAATA+TGG 0.287555 4.4:-77642621 MS.gene50301:CDS
AATTGTTGCCTTTGATTTAA+CGG 0.294431 4.4:-77642749 MS.gene50301:CDS
TTGTTTGAGATGCCTCTTCC+AGG 0.350585 4.4:-77642718 MS.gene50301:CDS
AGGAAATCTTAATTCTAGAT+TGG 0.414662 4.4:-77642479 MS.gene50301:CDS
CTATTAAGGCATCGCTCCTT+CGG 0.422078 4.4:-77642433 MS.gene50301:CDS
TTGGTCAAGTATAATGATAA+AGG 0.451105 4.4:-77642460 MS.gene50301:CDS
TAAACTTGCTCGTTGTCACC+AGG 0.464044 4.4:+77642361 None:intergenic
TAAGGATACAGTTGAAATAT+GGG 0.473963 4.4:-77642620 MS.gene50301:CDS
TTTCCTTTCCGTTAAATCAA+AGG 0.475134 4.4:+77642741 None:intergenic
TGGAGAATTTCTCAGTCTAT+GGG 0.489976 4.4:-77642656 MS.gene50301:CDS
AATACAAAGTGCATTCGTCT+TGG 0.503299 4.4:-77642588 MS.gene50301:CDS
ATGGGCTGTGGACTATGATA+AGG 0.510847 4.4:-77642638 MS.gene50301:CDS
ATAGGGTGAAAATAGCGATC+AGG 0.512584 4.4:+77642532 None:intergenic
GAGTCTCTACTTTCACTCCC+TGG 0.547203 4.4:-77642379 MS.gene50301:CDS
ATTTCTCAGTCTATGGGCTG+TGG 0.553738 4.4:-77642650 MS.gene50301:CDS
TTGCCTTTGATTTAACGGAA+AGG 0.569675 4.4:-77642744 MS.gene50301:CDS
TGGTTAAAATCACCTGGAAG+AGG 0.570122 4.4:+77642706 None:intergenic
GTGAAAATAGCGATCAGGAA+TGG 0.580924 4.4:+77642537 None:intergenic
AGTAAATGGTTAAAATCACC+TGG 0.587427 4.4:+77642700 None:intergenic
CTATGCGTCGTGATTTACGT+TGG 0.619317 4.4:-77642777 MS.gene50301:CDS
AAACTTGCTCGTTGTCACCA+GGG 0.636630 4.4:+77642362 None:intergenic
ATCGGATTGACTATTACCGA+AGG 0.667712 4.4:+77642417 None:intergenic
CTCTGTATACATGACAACAT+CGG 0.674049 4.4:+77642399 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACCAGAATTAAGTAAA+TGG + chr4.4:77642458-77642477 None:intergenic 20.0%
!! ACAAAAAATGGTGATATTAT+CGG - chr4.4:77642639-77642658 MS.gene50301:CDS 20.0%
!! TTTAACCATTTACTTAATTC+TGG - chr4.4:77642450-77642469 MS.gene50301:CDS 20.0%
!!! ATTTACTTAATTCTGGTTTT+TGG - chr4.4:77642457-77642476 MS.gene50301:CDS 20.0%
!!! TTTACTTAATTCTGGTTTTT+GGG - chr4.4:77642458-77642477 MS.gene50301:CDS 20.0%
! AGGAAATCTTAATTCTAGAT+TGG - chr4.4:77642662-77642681 MS.gene50301:CDS 25.0%
! ATAAGGATACAGTTGAAATA+TGG - chr4.4:77642520-77642539 MS.gene50301:CDS 25.0%
! TAAGGATACAGTTGAAATAT+GGG - chr4.4:77642521-77642540 MS.gene50301:CDS 25.0%
! TTGGTCAAGTATAATGATAA+AGG - chr4.4:77642681-77642700 MS.gene50301:CDS 25.0%
!! AATTGTTGCCTTTGATTTAA+CGG - chr4.4:77642392-77642411 MS.gene50301:CDS 25.0%
!!! GATAATATCACCATTTTTTG+TGG + chr4.4:77642640-77642659 None:intergenic 25.0%
AAAAATGGTGATATTATCGG+AGG - chr4.4:77642642-77642661 MS.gene50301:CDS 30.0%
AGTAAATGGTTAAAATCACC+TGG + chr4.4:77642444-77642463 None:intergenic 30.0%
ATGATAAAGGACAGCTATTA+AGG - chr4.4:77642694-77642713 MS.gene50301:CDS 30.0%
TTTCCTTTCCGTTAAATCAA+AGG + chr4.4:77642403-77642422 None:intergenic 30.0%
!!! AATTCTGGTTTTTGGGTATT+TGG - chr4.4:77642465-77642484 MS.gene50301:CDS 30.0%
!!! CATTTTTTGTGGAGTACATA+GGG + chr4.4:77642629-77642648 None:intergenic 30.0%
AATACAAAGTGCATTCGTCT+TGG - chr4.4:77642553-77642572 MS.gene50301:CDS 35.0%
CCTATGTACTCCACAAAAAA+TGG - chr4.4:77642627-77642646 MS.gene50301:CDS 35.0%
CTCTGTATACATGACAACAT+CGG + chr4.4:77642745-77642764 None:intergenic 35.0%
TGGAGAATTTCTCAGTCTAT+GGG - chr4.4:77642485-77642504 MS.gene50301:CDS 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr4.4:77642484-77642503 MS.gene50301:CDS 35.0%
! TTGCCTTTGATTTAACGGAA+AGG - chr4.4:77642397-77642416 MS.gene50301:CDS 35.0%
!!! CCATTTTTTGTGGAGTACAT+AGG + chr4.4:77642630-77642649 None:intergenic 35.0%
ATAGGGTGAAAATAGCGATC+AGG + chr4.4:77642612-77642631 None:intergenic 40.0%
ATCGGATTGACTATTACCGA+AGG + chr4.4:77642727-77642746 None:intergenic 40.0%
GTGAAAATAGCGATCAGGAA+TGG + chr4.4:77642607-77642626 None:intergenic 40.0%
TGGTTAAAATCACCTGGAAG+AGG + chr4.4:77642438-77642457 None:intergenic 40.0%
CTATGCGTCGTGATTTACGT+TGG - chr4.4:77642364-77642383 MS.gene50301:CDS 45.0%
! AAACTTGCTCGTTGTCACCA+GGG + chr4.4:77642782-77642801 None:intergenic 45.0%
! ATGGGCTGTGGACTATGATA+AGG - chr4.4:77642503-77642522 MS.gene50301:CDS 45.0%
! ATTTCTCAGTCTATGGGCTG+TGG - chr4.4:77642491-77642510 MS.gene50301:CDS 45.0%
! TAAACTTGCTCGTTGTCACC+AGG + chr4.4:77642783-77642802 None:intergenic 45.0%
! TTGTTTGAGATGCCTCTTCC+AGG - chr4.4:77642423-77642442 MS.gene50301:CDS 45.0%
!! CTATTAAGGCATCGCTCCTT+CGG - chr4.4:77642708-77642727 MS.gene50301:CDS 45.0%
GAGTCTCTACTTTCACTCCC+TGG - chr4.4:77642762-77642781 MS.gene50301:CDS 50.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 77642360 77642803 77642360 ID=MS.gene50301
chr4.4 mRNA 77642360 77642803 77642360 ID=MS.gene50301.t1;Parent=MS.gene50301
chr4.4 exon 77642360 77642803 77642360 ID=MS.gene50301.t1.exon1;Parent=MS.gene50301.t1
chr4.4 CDS 77642360 77642803 77642360 ID=cds.MS.gene50301.t1;Parent=MS.gene50301.t1
Gene Sequence

>MS.gene50301

ATGGCTATGCGTCGTGATTTACGTTGGAATGTAATTGTTGCCTTTGATTTAACGGAAAGGAAATTGTTTGAGATGCCTCTTCCAGGTGATTTTAACCATTTACTTAATTCTGGTTTTTGGGTATTTGGAGAATTTCTCAGTCTATGGGCTGTGGACTATGATAAGGATACAGTTGAAATATGGGTGATGAAAGAATACAAAGTGCATTCGTCTTGGAATAAAATTCTTGTTCTTTCTGTTCATGCCATTCCTGATCGCTATTTTCACCCTATGTACTCCACAAAAAATGGTGATATTATCGGAGGAAATCTTAATTCTAGATTGGTCAAGTATAATGATAAAGGACAGCTATTAAGGCATCGCTCCTTCGGTAATAGTCAATCCGATGTTGTCATGTATACAGAGTCTCTACTTTCACTCCCTGGTGACAACGAGCAAGTTTAA

Protein sequence

>MS.gene50301.t1

MAMRRDLRWNVIVAFDLTERKLFEMPLPGDFNHLLNSGFWVFGEFLSLWAVDYDKDTVEIWVMKEYKVHSSWNKILVLSVHAIPDRYFHPMYSTKNGDIIGGNLNSRLVKYNDKGQLLRHRSFGNSQSDVVMYTESLLSLPGDNEQV