AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77856

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77856.t1 MTR_3g034180 87.500 312 38 1 1 312 178 488 0.0 561
MS.gene77856.t1 MTR_3g034160 90.385 312 29 1 1 312 64 374 0.0 560
MS.gene77856.t1 MTR_3g034130 86.957 299 39 0 1 299 64 362 0.0 533
MS.gene77856.t1 MTR_3g103020 63.810 315 99 6 3 313 75 378 1.83e-133 384
MS.gene77856.t1 MTR_4g117720 62.229 323 98 9 1 313 70 378 5.18e-127 367
MS.gene77856.t1 MTR_6g060650 62.422 322 100 6 1 312 62 372 1.19e-124 361
MS.gene77856.t1 MTR_4g109270 57.367 319 112 7 1 313 64 364 6.53e-113 331
MS.gene77856.t1 MTR_4g122850 57.006 314 118 9 6 313 62 364 1.60e-109 323
MS.gene77856.t1 MTR_4g109280 61.093 311 102 8 6 313 65 359 3.42e-109 322
MS.gene77856.t1 MTR_4g117770 57.235 311 113 5 9 313 71 367 6.01e-107 316
MS.gene77856.t1 MTR_4g117810 57.235 311 113 5 9 313 71 367 6.01e-107 316
MS.gene77856.t1 MTR_4g117850 55.375 307 112 9 7 306 85 373 1.03e-100 301
MS.gene77856.t1 MTR_4g117780 54.487 312 120 7 9 313 71 367 1.60e-100 300
MS.gene77856.t1 MTR_4g094365 52.351 319 129 7 1 313 63 364 5.21e-100 298
MS.gene77856.t1 MTR_4g023080 54.140 314 118 8 1 312 64 353 1.94e-99 296
MS.gene77856.t1 MTR_4g023090 51.064 329 126 8 1 311 71 382 5.02e-99 296
MS.gene77856.t1 MTR_4g118380 54.636 302 105 6 6 286 67 357 1.25e-98 295
MS.gene77856.t1 MTR_4g021785 52.063 315 126 7 1 313 64 355 5.41e-97 290
MS.gene77856.t1 MTR_5g097210 51.148 305 133 6 7 310 71 360 8.71e-97 290
MS.gene77856.t1 MTR_5g070080 50.301 332 127 8 6 313 69 386 7.31e-94 283
MS.gene77856.t1 MTR_4g023380 48.974 341 114 9 1 313 71 379 2.19e-93 282
MS.gene77856.t1 MTR_4g116330 51.603 312 130 7 8 313 84 380 6.84e-93 281
MS.gene77856.t1 MTR_5g070130 50.156 321 134 10 6 313 70 377 2.84e-89 272
MS.gene77856.t1 MTR_4g023070 49.394 330 131 11 1 312 58 369 4.84e-89 271
MS.gene77856.t1 MTR_4g117870 50.318 314 108 7 1 313 1 267 8.09e-89 266
MS.gene77856.t1 MTR_4g117750 50.641 312 125 7 7 309 64 355 2.12e-87 270
MS.gene77856.t1 MTR_4g117750 61.468 109 40 1 205 311 373 481 5.82e-35 132
MS.gene77856.t1 MTR_4g023120 49.240 329 135 10 1 313 58 370 4.43e-87 266
MS.gene77856.t1 MTR_1g055135 48.254 315 143 8 7 313 73 375 6.47e-87 265
MS.gene77856.t1 MTR_4g023130 52.778 288 112 8 27 312 47 312 1.02e-85 260
MS.gene77856.t1 MTR_8g433040 50.641 312 121 9 9 313 79 364 4.93e-84 258
MS.gene77856.t1 MTR_8g032380 50.641 312 121 9 9 313 79 364 4.93e-84 258
MS.gene77856.t1 MTR_1g055195 47.188 320 138 7 2 313 182 478 5.13e-84 261
MS.gene77856.t1 MTR_4g023190 50.943 318 124 12 1 311 65 357 8.66e-83 254
MS.gene77856.t1 MTR_4g023200 49.686 318 129 12 1 311 65 358 3.22e-81 250
MS.gene77856.t1 MTR_4g023440 46.965 313 112 7 1 313 74 332 3.36e-80 247
MS.gene77856.t1 MTR_4g023150 48.580 317 134 11 1 311 65 358 5.87e-79 244
MS.gene77856.t1 MTR_5g070088 45.181 332 147 9 6 313 66 386 6.87e-79 245
MS.gene77856.t1 MTR_8g032390 48.544 309 115 10 7 308 99 370 1.79e-77 241
MS.gene77856.t1 MTR_4g118400 47.841 301 109 8 16 307 33 294 6.09e-77 237
MS.gene77856.t1 MTR_4g116320 57.589 224 80 6 91 310 43 255 5.80e-76 233
MS.gene77856.t1 MTR_8g040935 56.338 213 81 4 107 313 2 208 2.04e-72 223
MS.gene77856.t1 MTR_8g013010 58.333 216 73 5 1 214 109 309 2.87e-70 221
MS.gene77856.t1 MTR_4g118370 45.263 285 124 6 28 310 3 257 3.53e-69 216
MS.gene77856.t1 MTR_7g079400 42.395 309 155 8 14 313 43 337 8.48e-69 218
MS.gene77856.t1 MTR_4g121580 46.405 306 114 13 7 303 75 339 4.92e-66 212
MS.gene77856.t1 MTR_7g079370 42.500 320 148 11 7 307 48 350 1.15e-64 207
MS.gene77856.t1 MTR_8g433030 54.185 227 85 8 87 308 125 337 2.82e-64 206
MS.gene77856.t1 MTR_4g116330 43.590 312 116 8 8 313 84 341 2.40e-63 204
MS.gene77856.t1 MTR_6g463430 51.905 210 85 5 109 313 4 202 3.49e-56 181
MS.gene77856.t1 MTR_8g102520 40.705 312 143 11 7 313 48 322 3.77e-55 182
MS.gene77856.t1 MTR_1g055175 48.500 200 93 5 118 313 8 201 8.63e-54 174
MS.gene77856.t1 MTR_1g055250 42.857 231 117 8 74 300 34 253 9.80e-51 171
MS.gene77856.t1 MTR_4g117430 51.724 174 61 4 46 217 3 155 2.91e-50 164
MS.gene77856.t1 MTR_4g117530 42.745 255 94 8 63 313 47 253 3.92e-50 167
MS.gene77856.t1 MTR_1g055165 37.460 315 131 8 3 313 66 318 5.94e-48 163
MS.gene77856.t1 MTR_4g114700 39.777 269 101 6 42 304 5 218 1.70e-45 154
MS.gene77856.t1 MTR_4g116340 49.721 179 74 5 7 180 80 247 7.90e-44 150
MS.gene77856.t1 MTR_2g048580 34.194 310 158 12 10 313 73 342 4.46e-42 149
MS.gene77856.t1 MTR_4g118360 57.812 128 48 3 190 313 1 126 4.77e-41 139
MS.gene77856.t1 MTR_7g079420 33.333 285 129 8 45 313 905 1144 3.33e-38 144
MS.gene77856.t1 MTR_8g098830 43.103 174 85 5 114 282 30 194 1.45e-36 131
MS.gene77856.t1 MTR_4g122860 41.709 199 74 8 123 313 156 320 4.02e-36 133
MS.gene77856.t1 MTR_5g023000 66.667 93 31 0 221 313 7 99 5.90e-34 120
MS.gene77856.t1 MTR_7g117720 54.167 144 50 7 7 145 52 184 1.98e-32 121
MS.gene77856.t1 MTR_7g117720 50.000 100 45 3 213 307 172 271 1.01e-17 81.6
MS.gene77856.t1 MTR_6g034720 45.000 160 69 5 40 196 31 174 7.31e-31 115
MS.gene77856.t1 MTR_7g079450 41.060 151 64 5 174 313 1 137 2.28e-25 99.0
MS.gene77856.t1 MTR_1g068875 29.572 257 148 11 3 243 52 291 1.59e-23 99.8
MS.gene77856.t1 MTR_5g069120 44.961 129 62 2 6 127 51 177 2.20e-22 92.4
MS.gene77856.t1 MTR_0667s0010 26.190 294 176 10 3 268 54 334 2.99e-22 96.3
MS.gene77856.t1 MTR_2g028130 30.508 236 133 10 47 264 117 339 1.71e-21 94.4
MS.gene77856.t1 MTR_4g122840 41.912 136 41 4 181 313 164 264 9.61e-21 90.1
MS.gene77856.t1 MTR_4g133170 28.794 257 149 12 2 242 51 289 1.45e-20 91.3
MS.gene77856.t1 MTR_2g028160 28.846 260 141 10 44 274 107 351 1.65e-20 91.7
MS.gene77856.t1 MTR_2g024110 25.899 278 172 11 10 269 63 324 9.21e-20 89.0
MS.gene77856.t1 MTR_2g426070 30.594 219 125 8 57 261 129 334 1.34e-19 89.0
MS.gene77856.t1 MTR_4g023430 54.878 82 34 1 235 313 141 222 2.16e-19 85.5
MS.gene77856.t1 MTR_2g426020 24.314 255 157 7 44 276 101 341 2.33e-19 88.2
MS.gene77856.t1 MTR_2g026010 27.734 256 161 10 35 276 81 326 7.00e-19 86.7
MS.gene77856.t1 MTR_4g070550 27.273 286 158 13 3 250 53 326 1.81e-18 85.5
MS.gene77856.t1 MTR_2g025960 26.199 271 157 10 39 282 1 255 1.84e-18 84.3
MS.gene77856.t1 MTR_8g013005 67.797 59 19 0 255 313 1 59 4.79e-18 77.0
MS.gene77856.t1 MTR_2g031640 26.667 225 140 6 33 242 81 295 5.35e-18 84.0
MS.gene77856.t1 MTR_2g426090 25.475 263 156 11 37 276 94 339 1.58e-17 82.8
MS.gene77856.t1 MTR_1g055200 47.573 103 43 5 42 139 5 101 2.26e-17 76.6
MS.gene77856.t1 MTR_2g078870 32.836 201 103 11 44 238 58 232 2.81e-17 81.3
MS.gene77856.t1 MTR_2g025420 24.522 314 181 12 4 274 56 356 6.99e-17 80.9
MS.gene77856.t1 MTR_5g037680 26.502 283 171 10 38 305 121 381 1.08e-16 80.5
MS.gene77856.t1 MTR_2g028140 28.085 235 130 10 44 250 108 331 1.61e-16 80.1
MS.gene77856.t1 MTR_1g068835 27.572 243 148 11 16 243 63 292 2.21e-16 79.3
MS.gene77856.t1 MTR_7g070440 28.197 305 165 12 17 285 71 357 2.47e-16 79.0
MS.gene77856.t1 MTR_1g068860 24.797 246 160 7 12 243 60 294 7.12e-16 77.8
MS.gene77856.t1 MTR_3g083640 26.280 293 180 11 23 305 136 402 8.65e-16 77.8
MS.gene77856.t1 MTR_2g021770 27.160 324 193 13 17 308 93 405 1.32e-15 77.0
MS.gene77856.t1 MTR_2g031720 24.194 310 192 11 2 280 43 340 4.14e-15 75.5
MS.gene77856.t1 MTR_3g083010 25.094 267 162 9 37 276 102 357 4.84e-15 75.5
MS.gene77856.t1 MTR_2g021155 28.095 210 129 7 37 238 687 882 5.89e-15 75.9
MS.gene77856.t1 MTR_2g021155 29.956 227 138 9 22 242 110 321 8.21e-14 72.4
MS.gene77856.t1 MTR_2g425980 28.821 229 130 9 54 262 119 334 6.09e-15 75.1
MS.gene77856.t1 MTR_4g031770 29.808 208 118 10 44 242 101 289 6.38e-15 75.1
MS.gene77856.t1 MTR_2g021150 27.413 259 162 10 15 264 87 328 7.82e-15 74.7
MS.gene77856.t1 MTR_2g031680 26.923 234 144 9 23 242 72 292 8.26e-15 74.7
MS.gene77856.t1 MTR_4g117550 28.467 274 147 11 53 311 135 374 1.01e-14 74.3
MS.gene77856.t1 MTR_2g024100 30.374 214 113 11 47 238 107 306 1.42e-14 73.9
MS.gene77856.t1 MTR_4g030830 23.762 303 168 12 44 305 113 393 3.22e-14 73.2
MS.gene77856.t1 MTR_6g053240 26.087 207 129 6 41 238 125 316 3.52e-14 72.8
MS.gene77856.t1 MTR_2g461450 26.786 224 138 8 32 248 97 301 3.88e-14 72.4
MS.gene77856.t1 MTR_6g065980 29.577 213 120 10 44 252 111 297 3.97e-14 72.4
MS.gene77856.t1 MTR_2g028110 26.721 247 145 10 23 246 70 303 4.00e-14 72.8
MS.gene77856.t1 MTR_3g030250 26.071 280 157 10 46 305 130 379 4.74e-14 72.4
MS.gene77856.t1 MTR_4g055060 27.419 248 148 10 9 243 94 322 5.71e-14 72.4
MS.gene77856.t1 MTR_5g068490 24.662 296 183 12 20 304 5 271 8.29e-14 70.9
MS.gene77856.t1 MTR_8g099460 24.671 304 174 11 4 283 56 328 1.05e-13 71.6
MS.gene77856.t1 MTR_1g094210 27.692 260 141 12 40 284 151 378 1.14e-13 71.6
MS.gene77856.t1 MTR_7g056390 27.273 286 140 12 41 285 130 388 1.86e-13 70.9
MS.gene77856.t1 MTR_7g070830 25.630 238 138 9 57 264 147 375 3.19e-13 70.1
MS.gene77856.t1 MTR_1g054725 29.808 208 116 10 43 238 117 306 3.51e-13 69.7
MS.gene77856.t1 MTR_0044s0300 28.293 205 110 8 44 238 138 315 4.67e-13 69.7
MS.gene77856.t1 MTR_6g053260 28.302 212 125 8 34 238 429 620 5.16e-13 70.1
MS.gene77856.t1 MTR_4g023100 64.286 56 18 1 257 312 65 118 5.65e-13 65.1
MS.gene77856.t1 MTR_4g061340 28.169 213 124 6 44 243 120 316 6.07e-13 68.9
MS.gene77856.t1 MTR_5g097680 25.442 283 162 12 59 308 159 425 6.77e-13 69.3
MS.gene77856.t1 MTR_5g064700 26.207 290 153 14 59 308 98 366 7.11e-13 68.9
MS.gene77856.t1 MTR_0129s0050 26.490 302 167 12 47 308 120 406 8.41e-13 68.9
MS.gene77856.t1 MTR_2g078900 30.055 183 99 9 59 238 153 309 9.29e-13 68.6
MS.gene77856.t1 MTR_8g467670 26.275 255 157 9 59 303 139 372 1.10e-12 68.6
MS.gene77856.t1 MTR_3g072470 26.761 284 147 12 41 286 130 390 1.24e-12 68.2
MS.gene77856.t1 MTR_3g007330 26.066 211 125 7 44 243 138 328 1.29e-12 68.2
MS.gene77856.t1 MTR_1g094210 27.559 254 137 12 40 278 151 372 1.48e-12 68.2
MS.gene77856.t1 MTR_3g010970 26.642 274 158 11 41 305 152 391 1.61e-12 67.8
MS.gene77856.t1 MTR_7g077910 25.652 230 137 7 41 251 42 256 1.71e-12 67.4
MS.gene77856.t1 MTR_2g043410 29.258 229 123 9 40 251 133 339 1.72e-12 67.8
MS.gene77856.t1 MTR_1g097560 25.670 261 160 11 28 281 119 352 2.08e-12 67.4
MS.gene77856.t1 MTR_0054s0090 25.874 286 167 10 57 311 136 407 2.28e-12 67.4
MS.gene77856.t1 MTR_0054s0100 26.073 303 174 12 44 311 122 409 2.38e-12 67.4
MS.gene77856.t1 MTR_6g027910 26.636 214 131 6 38 238 106 306 2.81e-12 67.0
MS.gene77856.t1 MTR_3g007430 27.477 222 134 8 32 242 123 328 4.01e-12 66.6
MS.gene77856.t1 MTR_7g017040 24.834 302 183 13 34 306 108 394 4.13e-12 66.6
MS.gene77856.t1 MTR_3g009310 26.943 193 112 6 57 243 158 327 6.03e-12 66.2
MS.gene77856.t1 MTR_1g055145 45.946 74 38 2 157 229 130 202 6.72e-12 65.1
MS.gene77856.t1 MTR_3g007410 27.751 209 127 8 44 243 146 339 6.78e-12 66.2
MS.gene77856.t1 MTR_8g464770 25.325 308 195 11 17 307 88 377 7.28e-12 65.9
MS.gene77856.t1 MTR_7g056290 26.481 287 141 11 41 285 134 392 8.65e-12 65.9
MS.gene77856.t1 MTR_3g023930 26.838 272 155 11 44 305 130 367 9.29e-12 65.5
MS.gene77856.t1 MTR_3g007360 27.751 209 127 8 44 243 146 339 1.03e-11 65.5
MS.gene77856.t1 MTR_7g056503 25.939 293 179 14 40 305 116 397 1.06e-11 65.5
MS.gene77856.t1 MTR_5g057690 30.288 208 122 9 40 242 131 320 1.16e-11 65.5
MS.gene77856.t1 MTR_0129s0070 25.899 278 169 11 57 312 136 398 1.22e-11 65.5
MS.gene77856.t1 MTR_3g010870 27.317 205 118 9 41 238 131 311 1.26e-11 65.1
MS.gene77856.t1 MTR_7g057270 25.957 235 134 10 41 248 123 344 1.45e-11 65.1
MS.gene77856.t1 MTR_4g109290 60.377 53 21 0 261 313 136 188 1.48e-11 62.8
MS.gene77856.t1 MTR_3g030320 26.295 251 144 13 1 238 1 223 2.43e-11 63.9
MS.gene77856.t1 MTR_3g006500 25.540 278 167 10 39 305 121 369 2.75e-11 63.9
MS.gene77856.t1 MTR_4g108820 27.126 247 133 11 30 260 96 311 2.86e-11 63.5
MS.gene77856.t1 MTR_2g038680 25.000 308 169 14 42 308 123 409 3.00e-11 64.3
MS.gene77856.t1 MTR_2g029990 26.383 235 142 12 15 238 120 334 3.16e-11 63.9
MS.gene77856.t1 MTR_6g007135 26.106 226 139 9 38 252 121 329 3.45e-11 63.9
MS.gene77856.t1 MTR_6g065510 23.729 236 138 8 54 264 71 289 4.08e-11 63.5
MS.gene77856.t1 MTR_3g010430 28.230 209 113 10 44 238 133 318 4.56e-11 63.5
MS.gene77856.t1 MTR_4g031020 25.000 260 148 10 18 246 114 357 4.94e-11 63.5
MS.gene77856.t1 MTR_3g024130 26.484 219 138 7 41 252 58 260 5.89e-11 62.8
MS.gene77856.t1 MTR_4g037465 26.522 230 127 10 57 264 140 349 6.02e-11 63.2
MS.gene77856.t1 MTR_6g007150 28.455 246 145 11 7 238 33 261 6.48e-11 62.8
MS.gene77856.t1 MTR_7g445890 25.532 235 142 9 41 251 87 312 6.87e-11 62.8
MS.gene77856.t1 MTR_3g006570 25.000 284 166 10 44 305 118 376 7.26e-11 62.8
MS.gene77856.t1 MTR_7g060960 26.667 225 112 11 41 243 106 299 7.39e-11 62.8
MS.gene77856.t1 MTR_7g056003 26.293 232 132 8 41 251 118 331 7.40e-11 62.8
MS.gene77856.t1 MTR_4g013180 25.641 234 136 7 44 251 105 326 7.84e-11 63.2
MS.gene77856.t1 MTR_3g096280 26.523 279 154 18 37 305 105 342 7.89e-11 62.8
MS.gene77856.t1 MTR_3g464470 24.231 260 138 9 45 267 103 340 8.11e-11 62.8
MS.gene77856.t1 MTR_3g011120 26.609 233 142 9 54 276 133 346 8.13e-11 62.8
MS.gene77856.t1 MTR_5g086590 26.263 198 123 7 54 242 11 194 8.38e-11 61.6
MS.gene77856.t1 MTR_5g022040 25.197 254 144 11 18 247 161 392 8.52e-11 62.8
MS.gene77856.t1 MTR_2g021252 26.633 199 115 6 44 238 145 316 8.71e-11 62.8
MS.gene77856.t1 MTR_5g059430 25.589 297 169 13 44 310 152 426 8.82e-11 62.8
MS.gene77856.t1 MTR_2g064960 64.286 42 15 0 272 313 18 59 9.25e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77856.t1 AT3G06240 29.020 255 140 12 20 260 145 372 6.90e-19 87.0
MS.gene77856.t1 AT4G12560 29.249 253 161 10 40 276 90 340 1.67e-17 82.8
MS.gene77856.t1 AT4G12560 29.249 253 161 10 40 276 90 340 1.67e-17 82.8
MS.gene77856.t1 AT4G12560 29.249 253 161 10 40 276 90 340 1.67e-17 82.8
MS.gene77856.t1 AT4G22390 24.746 295 191 12 40 313 90 374 1.74e-13 70.9
MS.gene77856.t1 AT4G22390 24.746 295 191 12 40 313 90 374 1.92e-13 70.9

Find 70 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTTGCATTCATATAATA+AGG 0.164755 3.4:+25747784 None:intergenic
TTGGAGAATTTCTAAGTCTA+TGG 0.196739 3.4:-25747591 MS.gene77856:CDS
GATTGTGATTTGTGGATATT+TGG 0.226311 3.4:-25747610 MS.gene77856:CDS
TCATGTTGTCTGTATGGTTT+TGG 0.229471 3.4:-25747970 MS.gene77856:CDS
AGGATGGAACAATTGAAATA+TGG 0.245076 3.4:-25747561 MS.gene77856:CDS
ATTCGGGAAGCATAAAATTA+AGG 0.275163 3.4:+25748133 None:intergenic
GTTCTTCAAACATTTATCTA+TGG 0.300796 3.4:-25748056 MS.gene77856:CDS
GATGAAATATTATCTTCTAT+TGG 0.305333 3.4:+25747886 None:intergenic
AAAGTAGGGACGCTCTTTAA+TGG 0.309047 3.4:-25747754 MS.gene77856:CDS
TGAATATTGGCCATCTAAAC+TGG 0.318220 3.4:+25747990 None:intergenic
ATGAAATATTATCTTCTATT+GGG 0.319010 3.4:+25747887 None:intergenic
AGATAATATTTCATCACATT+TGG 0.320818 3.4:-25747878 MS.gene77856:CDS
AAGCGATGCTTCAAAATCTA+TGG 0.326989 3.4:+25748185 None:intergenic
ATTAAAGAGCGTCCCTACTT+TGG 0.358272 3.4:+25747756 None:intergenic
TTCAAGATTAGAATAAGATT+CGG 0.367334 3.4:+25748116 None:intergenic
TTTCCTCCTTCCAGTTTAGA+TGG 0.367355 3.4:-25748000 MS.gene77856:CDS
CATACAGACAACATGAATAT+TGG 0.369761 3.4:+25747977 None:intergenic
TATTGTTGGCTTTGATTTAG+TGG 0.370118 3.4:-25747686 MS.gene77856:CDS
TTAAAGAGCGTCCCTACTTT+GGG 0.374951 3.4:+25747757 None:intergenic
TCTTTAATGGGACTATTCAT+TGG 0.384035 3.4:-25747741 MS.gene77856:CDS
CAATATTCATGTTGTCTGTA+TGG 0.390156 3.4:-25747976 MS.gene77856:CDS
TCAAGATTAGAATAAGATTC+GGG 0.401464 3.4:+25748117 None:intergenic
TGGAAATGTCATCAATAGAT+AGG 0.410119 3.4:+25747491 None:intergenic
CCATTCAAGTGATGATTACT+TGG 0.412169 3.4:-25747941 MS.gene77856:CDS
AACCTATAGATTGTGATTTG+TGG 0.425796 3.4:-25747618 MS.gene77856:CDS
AAGTAGGGACGCTCTTTAAT+GGG 0.430028 3.4:-25747753 MS.gene77856:CDS
AGGTATTTGTTTGTGATGTC+CGG 0.430912 3.4:+25748023 None:intergenic
GGATGGAACAATTGAAATAT+GGG 0.445935 3.4:-25747560 MS.gene77856:CDS
CATTTGCATTCATATAATAA+GGG 0.448554 3.4:+25747785 None:intergenic
GAGTCTCTGCTTTCACTCCC+TGG 0.452167 3.4:-25747313 MS.gene77856:CDS
AATACAAAGTGCATTCGTCT+TGG 0.453817 3.4:-25747528 MS.gene77856:CDS
AATCACAATCTATAGGTTCA+TGG 0.456461 3.4:+25747623 None:intergenic
ATACAAAGTGCATTCGTCTT+GGG 0.456469 3.4:-25747527 MS.gene77856:CDS
GGTTCATGGTCAAAACCATC+TGG 0.456773 3.4:+25747637 None:intergenic
ATCCACAAATCACAATCTAT+AGG 0.460799 3.4:+25747616 None:intergenic
TGGGTCTTTACTCGTATCAT+AGG 0.461190 3.4:+25747906 None:intergenic
ATTGGAACAAACGGTGGCAC+TGG 0.477184 3.4:-25747424 MS.gene77856:CDS
TTATCAATGGATGTTATTGT+TGG 0.479970 3.4:-25747700 MS.gene77856:CDS
CTCTGTATACATAGCCACTA+GGG 0.483092 3.4:+25747333 None:intergenic
TCTAAACTGGAAGGAGGAAA+AGG 0.489830 3.4:+25748003 None:intergenic
TGGAGAATTTCTAAGTCTAT+GGG 0.490268 3.4:-25747590 MS.gene77856:CDS
TGATTATACTTCACCGATCC+AGG 0.490650 3.4:+25747811 None:intergenic
ATTTCTAAGTCTATGGGCTA+TGG 0.507502 3.4:-25747584 MS.gene77856:CDS
TGGTCAAAACCATCTGGAAA+AGG 0.508561 3.4:+25747643 None:intergenic
GCAAATGATGATCCCAAAGT+AGG 0.511115 3.4:-25747769 MS.gene77856:CDS
TGGAAAGATATCGAGTGTCC+TGG 0.527209 3.4:-25747829 MS.gene77856:CDS
TTTCATTGAGAGCTAATACA+TGG 0.531728 3.4:-25747849 MS.gene77856:CDS
AGTCTATGGGCTATGGAGGA+TGG 0.532601 3.4:-25747577 MS.gene77856:CDS
CCAAGTAATCATCACTTGAA+TGG 0.535251 3.4:+25747941 None:intergenic
GAAATTAAAAGTTCTTGTAG+AGG 0.536250 3.4:-25748096 MS.gene77856:CDS
GGTGGTATTATTGGAACAAA+CGG 0.557309 3.4:-25747433 MS.gene77856:CDS
TTGGCTTTGATTTAGTGGAA+AGG 0.565737 3.4:-25747681 MS.gene77856:CDS
CAAATGATGATCCCAAAGTA+GGG 0.565983 3.4:-25747768 MS.gene77856:CDS
TTCAAGTGATGATTACTTGG+TGG 0.566498 3.4:-25747938 MS.gene77856:CDS
AACAAACGGTGGCACTGGAT+TGG 0.570072 3.4:-25747419 MS.gene77856:CDS
TCTAAGTCTATGGGCTATGG+AGG 0.577648 3.4:-25747581 MS.gene77856:CDS
TGTTTGTGATGTCCGGTCGA+TGG 0.582419 3.4:+25748030 None:intergenic
TAAGCTTGATCACTGTCACC+AGG 0.583359 3.4:+25747295 None:intergenic
ACTGGATTGGTGAAGTATGA+TGG 0.588559 3.4:-25747406 MS.gene77856:CDS
AGATATCGAGTGTCCTGGAT+CGG 0.591831 3.4:-25747824 MS.gene77856:CDS
TATCTATGGAATCCATCGAC+CGG 0.592196 3.4:-25748042 MS.gene77856:CDS
AAGCTTGATCACTGTCACCA+GGG 0.598347 3.4:+25747296 None:intergenic
TATTGGCCATCTAAACTGGA+AGG 0.609203 3.4:+25747994 None:intergenic
ACTCTGTATACATAGCCACT+AGG 0.618200 3.4:+25747332 None:intergenic
TGGCCATCTAAACTGGAAGG+AGG 0.648530 3.4:+25747997 None:intergenic
CGAACCATGTGAACCCCTAG+TGG 0.657467 3.4:-25747347 MS.gene77856:CDS
TCTGTATACATAGCCACTAG+GGG 0.693139 3.4:+25747334 None:intergenic
ATAGCCACTAGGGGTTCACA+TGG 0.697094 3.4:+25747343 None:intergenic
GGTATTATTGGAACAAACGG+TGG 0.700731 3.4:-25747430 MS.gene77856:CDS
TTGGTGAAGTATGATGGCAA+TGG 0.739876 3.4:-25747400 MS.gene77856:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAATATTATCTTCTATT+GGG + chr3.4:25747623-25747642 None:intergenic 15.0%
!! AGATAATATTTCATCACATT+TGG - chr3.4:25747629-25747648 MS.gene77856:CDS 20.0%
!! CATTTGCATTCATATAATAA+GGG + chr3.4:25747725-25747744 None:intergenic 20.0%
!! GATGAAATATTATCTTCTAT+TGG + chr3.4:25747624-25747643 None:intergenic 20.0%
!! TCATTTGCATTCATATAATA+AGG + chr3.4:25747726-25747745 None:intergenic 20.0%
!! TTCAAGATTAGAATAAGATT+CGG + chr3.4:25747394-25747413 None:intergenic 20.0%
! GAAATTAAAAGTTCTTGTAG+AGG - chr3.4:25747411-25747430 MS.gene77856:CDS 25.0%
! GTTCTTCAAACATTTATCTA+TGG - chr3.4:25747451-25747470 MS.gene77856:CDS 25.0%
! TCAAGATTAGAATAAGATTC+GGG + chr3.4:25747393-25747412 None:intergenic 25.0%
! TTATCAATGGATGTTATTGT+TGG - chr3.4:25747807-25747826 MS.gene77856:CDS 25.0%
!! ACTTTTTGTAGAACATATTG+GGG + chr3.4:25748054-25748073 None:intergenic 25.0%
!! TTTTCGCAATGATTTATCAA+TGG - chr3.4:25747794-25747813 MS.gene77856:CDS 25.0%
!!! CACTTTTTGTAGAACATATT+GGG + chr3.4:25748055-25748074 None:intergenic 25.0%
AACCTATAGATTGTGATTTG+TGG - chr3.4:25747889-25747908 MS.gene77856:CDS 30.0%
AATCACAATCTATAGGTTCA+TGG + chr3.4:25747887-25747906 None:intergenic 30.0%
ACAAAAAGTGGTGGTATTAT+TGG - chr3.4:25748065-25748084 MS.gene77856:CDS 30.0%
AGGATGGAACAATTGAAATA+TGG - chr3.4:25747946-25747965 MS.gene77856:CDS 30.0%
ATATGTTCTACAAAAAGTGG+TGG - chr3.4:25748056-25748075 MS.gene77856:CDS 30.0%
ATCCACAAATCACAATCTAT+AGG + chr3.4:25747894-25747913 None:intergenic 30.0%
CAATATTCATGTTGTCTGTA+TGG - chr3.4:25747531-25747550 MS.gene77856:CDS 30.0%
CATACAGACAACATGAATAT+TGG + chr3.4:25747533-25747552 None:intergenic 30.0%
CCAATATGTTCTACAAAAAG+TGG - chr3.4:25748053-25748072 MS.gene77856:CDS 30.0%
GGATGGAACAATTGAAATAT+GGG - chr3.4:25747947-25747966 MS.gene77856:CDS 30.0%
TCTTTAATGGGACTATTCAT+TGG - chr3.4:25747766-25747785 MS.gene77856:CDS 30.0%
TGGAAATGTCATCAATAGAT+AGG + chr3.4:25748019-25748038 None:intergenic 30.0%
TGGAGAATTTCTAAGTCTAT+GGG - chr3.4:25747917-25747936 MS.gene77856:CDS 30.0%
TTGGAGAATTTCTAAGTCTA+TGG - chr3.4:25747916-25747935 MS.gene77856:CDS 30.0%
TTTCATTGAGAGCTAATACA+TGG - chr3.4:25747658-25747677 MS.gene77856:CDS 30.0%
! ATTCGGGAAGCATAAAATTA+AGG + chr3.4:25747377-25747396 None:intergenic 30.0%
! CTTTTTGTAGAACATATTGG+GGG + chr3.4:25748053-25748072 None:intergenic 30.0%
! GATTGTGATTTGTGGATATT+TGG - chr3.4:25747897-25747916 MS.gene77856:CDS 30.0%
!! TATTGTTGGCTTTGATTTAG+TGG - chr3.4:25747821-25747840 MS.gene77856:CDS 30.0%
!!! CCACTTTTTGTAGAACATAT+TGG + chr3.4:25748056-25748075 None:intergenic 30.0%
AAGCGATGCTTCAAAATCTA+TGG + chr3.4:25747325-25747344 None:intergenic 35.0%
AATACAAAGTGCATTCGTCT+TGG - chr3.4:25747979-25747998 MS.gene77856:CDS 35.0%
AGGTATTTGTTTGTGATGTC+CGG + chr3.4:25747487-25747506 None:intergenic 35.0%
ATACAAAGTGCATTCGTCTT+GGG - chr3.4:25747980-25747999 MS.gene77856:CDS 35.0%
CAAATGATGATCCCAAAGTA+GGG - chr3.4:25747739-25747758 MS.gene77856:CDS 35.0%
CCAAGTAATCATCACTTGAA+TGG + chr3.4:25747569-25747588 None:intergenic 35.0%
CCATTCAAGTGATGATTACT+TGG - chr3.4:25747566-25747585 MS.gene77856:CDS 35.0%
GGTGGTATTATTGGAACAAA+CGG - chr3.4:25748074-25748093 MS.gene77856:CDS 35.0%
TGAATATTGGCCATCTAAAC+TGG + chr3.4:25747520-25747539 None:intergenic 35.0%
TTCAAGTGATGATTACTTGG+TGG - chr3.4:25747569-25747588 MS.gene77856:CDS 35.0%
! ATTTCTAAGTCTATGGGCTA+TGG - chr3.4:25747923-25747942 MS.gene77856:CDS 35.0%
! CATGTTGTCTGTATGGTTTT+GGG - chr3.4:25747538-25747557 MS.gene77856:CDS 35.0%
! TCATGTTGTCTGTATGGTTT+TGG - chr3.4:25747537-25747556 MS.gene77856:CDS 35.0%
!! TTGGCTTTGATTTAGTGGAA+AGG - chr3.4:25747826-25747845 MS.gene77856:CDS 35.0%
AAAGTAGGGACGCTCTTTAA+TGG - chr3.4:25747753-25747772 MS.gene77856:CDS 40.0%
AAGTAGGGACGCTCTTTAAT+GGG - chr3.4:25747754-25747773 MS.gene77856:CDS 40.0%
ACTCTGTATACATAGCCACT+AGG + chr3.4:25748178-25748197 None:intergenic 40.0%
CTCTGTATACATAGCCACTA+GGG + chr3.4:25748177-25748196 None:intergenic 40.0%
GCAAATGATGATCCCAAAGT+AGG - chr3.4:25747738-25747757 MS.gene77856:CDS 40.0%
GGTATTATTGGAACAAACGG+TGG - chr3.4:25748077-25748096 MS.gene77856:CDS 40.0%
TATCTATGGAATCCATCGAC+CGG - chr3.4:25747465-25747484 MS.gene77856:CDS 40.0%
TATTGGCCATCTAAACTGGA+AGG + chr3.4:25747516-25747535 None:intergenic 40.0%
TATTGGGGGATAGTACTCAA+TGG + chr3.4:25748039-25748058 None:intergenic 40.0%
TCTAAACTGGAAGGAGGAAA+AGG + chr3.4:25747507-25747526 None:intergenic 40.0%
TCTGTATACATAGCCACTAG+GGG + chr3.4:25748176-25748195 None:intergenic 40.0%
TGATTATACTTCACCGATCC+AGG + chr3.4:25747699-25747718 None:intergenic 40.0%
TGGGTCTTTACTCGTATCAT+AGG + chr3.4:25747604-25747623 None:intergenic 40.0%
TGGTCAAAACCATCTGGAAA+AGG + chr3.4:25747867-25747886 None:intergenic 40.0%
TTGGTGAAGTATGATGGCAA+TGG - chr3.4:25748107-25748126 MS.gene77856:CDS 40.0%
TTTCCTCCTTCCAGTTTAGA+TGG - chr3.4:25747507-25747526 MS.gene77856:CDS 40.0%
!! ACTGGATTGGTGAAGTATGA+TGG - chr3.4:25748101-25748120 MS.gene77856:CDS 40.0%
!! ATTAAAGAGCGTCCCTACTT+TGG + chr3.4:25747754-25747773 None:intergenic 40.0%
!! TTAAAGAGCGTCCCTACTTT+GGG + chr3.4:25747753-25747772 None:intergenic 40.0%
AAGCTTGATCACTGTCACCA+GGG + chr3.4:25748214-25748233 None:intergenic 45.0%
AGATATCGAGTGTCCTGGAT+CGG - chr3.4:25747683-25747702 MS.gene77856:CDS 45.0%
GGTTCATGGTCAAAACCATC+TGG + chr3.4:25747873-25747892 None:intergenic 45.0%
TAAGCTTGATCACTGTCACC+AGG + chr3.4:25748215-25748234 None:intergenic 45.0%
TGGAAAGATATCGAGTGTCC+TGG - chr3.4:25747678-25747697 MS.gene77856:CDS 45.0%
! TCTAAGTCTATGGGCTATGG+AGG - chr3.4:25747926-25747945 MS.gene77856:CDS 45.0%
! TTCGAGATGCCTTTTCCAGA+TGG - chr3.4:25747855-25747874 MS.gene77856:CDS 45.0%
ATAGCCACTAGGGGTTCACA+TGG + chr3.4:25748167-25748186 None:intergenic 50.0%
ATTGGAACAAACGGTGGCAC+TGG - chr3.4:25748083-25748102 MS.gene77856:CDS 50.0%
TGGCCATCTAAACTGGAAGG+AGG + chr3.4:25747513-25747532 None:intergenic 50.0%
TGTTTGTGATGTCCGGTCGA+TGG + chr3.4:25747480-25747499 None:intergenic 50.0%
! AACAAACGGTGGCACTGGAT+TGG - chr3.4:25748088-25748107 MS.gene77856:CDS 50.0%
! AGTCTATGGGCTATGGAGGA+TGG - chr3.4:25747930-25747949 MS.gene77856:CDS 50.0%
CGAACCATGTGAACCCCTAG+TGG - chr3.4:25748160-25748179 MS.gene77856:CDS 55.0%
GAGTCTCTGCTTTCACTCCC+TGG - chr3.4:25748194-25748213 MS.gene77856:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 25747294 25748235 25747294 ID=MS.gene77856
chr3.4 mRNA 25747294 25748235 25747294 ID=MS.gene77856.t1;Parent=MS.gene77856
chr3.4 exon 25747294 25748235 25747294 ID=MS.gene77856.t1.exon1;Parent=MS.gene77856.t1
chr3.4 CDS 25747294 25748235 25747294 ID=cds.MS.gene77856.t1;Parent=MS.gene77856.t1
Gene Sequence

>MS.gene77856

ATGTCAACTCTAGCTCTTGAAACTCGATCCATAGATTTTGAAGCATCGCTTAACGATGATTCTGCTTCTACTTCACTGAACCTTAATTTTATGCTTCCCGAATCTTATTCTAATCTTGAAATTAAAAGTTCTTGTAGAGGATTTATAGTTTTGACTTGTTCTTCAAACATTTATCTATGGAATCCATCGACCGGACATCACAAACAAATACCTTTTCCTCCTTCCAGTTTAGATGGCCAATATTCATGTTGTCTGTATGGTTTTGGGTACGACCATTCAAGTGATGATTACTTGGTGGTATCAGTGTCCTATGATACGAGTAAAGACCCAATAGAAGATAATATTTCATCACATTTGGAGTTTTTTTCATTGAGAGCTAATACATGGAAAGATATCGAGTGTCCTGGATCGGTGAAGTATAATCACTTCCCTTATTATATGAATGCAAATGATGATCCCAAAGTAGGGACGCTCTTTAATGGGACTATTCATTGGTTCTCTTTTCGCAATGATTTATCAATGGATGTTATTGTTGGCTTTGATTTAGTGGAAAGGAAACTTTTCGAGATGCCTTTTCCAGATGGTTTTGACCATGAACCTATAGATTGTGATTTGTGGATATTTGGAGAATTTCTAAGTCTATGGGCTATGGAGGATGGAACAATTGAAATATGGGTTATGAAAGAATACAAAGTGCATTCGTCTTGGGTTAAAACTCTTGTCCTATCTATTGATGACATTTCCATTGAGTACTATCCCCCAATATGTTCTACAAAAAGTGGTGGTATTATTGGAACAAACGGTGGCACTGGATTGGTGAAGTATGATGGCAATGGTCAGTTGCTAGAGAATCGCTCATACTATAACGAACCATGTGAACCCCTAGTGGCTATGTATACAGAGTCTCTGCTTTCACTCCCTGGTGACAGTGATCAAGCTTAA
Protein sequence

>MS.gene77856.t1

MSTLALETRSIDFEASLNDDSASTSLNLNFMLPESYSNLEIKSSCRGFIVLTCSSNIYLWNPSTGHHKQIPFPPSSLDGQYSCCLYGFGYDHSSDDYLVVSVSYDTSKDPIEDNISSHLEFFSLRANTWKDIECPGSVKYNHFPYYMNANDDPKVGTLFNGTIHWFSFRNDLSMDVIVGFDLVERKLFEMPFPDGFDHEPIDCDLWIFGEFLSLWAMEDGTIEIWVMKEYKVHSSWVKTLVLSIDDISIEYYPPICSTKSGGIIGTNGGTGLVKYDGNGQLLENRSYYNEPCEPLVAMYTESLLSLPGDSDQA