AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44883

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44883.t1 MTR_5g070080 87.591 274 34 0 6 279 113 386 2.07e-178 497
MS.gene44883.t1 MTR_5g070088 67.266 278 86 4 6 279 110 386 2.11e-127 367
MS.gene44883.t1 MTR_5g070130 64.158 279 90 4 10 279 100 377 1.11e-118 345
MS.gene44883.t1 MTR_4g117720 55.357 280 111 7 10 279 103 378 3.05e-93 280
MS.gene44883.t1 MTR_3g103020 54.255 282 123 5 1 279 100 378 1.05e-91 276
MS.gene44883.t1 MTR_5g097210 52.313 281 113 9 2 273 90 358 3.02e-83 254
MS.gene44883.t1 MTR_6g060650 51.852 270 121 6 13 278 108 372 4.60e-82 251
MS.gene44883.t1 MTR_1g055195 51.799 278 108 8 12 279 218 479 9.75e-81 251
MS.gene44883.t1 MTR_3g034180 49.315 292 128 7 1 279 205 489 3.09e-80 250
MS.gene44883.t1 MTR_1g055135 53.004 283 115 10 2 273 95 370 5.30e-80 246
MS.gene44883.t1 MTR_3g034160 50.690 290 122 8 1 276 91 373 6.61e-79 243
MS.gene44883.t1 MTR_4g109270 52.518 278 110 7 12 279 99 364 6.71e-79 243
MS.gene44883.t1 MTR_4g109280 51.064 282 129 6 1 279 84 359 6.14e-78 241
MS.gene44883.t1 MTR_4g023080 50.000 276 124 7 1 275 89 351 3.35e-76 236
MS.gene44883.t1 MTR_4g023090 46.644 298 132 9 1 279 97 386 1.63e-75 235
MS.gene44883.t1 MTR_4g094365 48.399 281 132 8 5 279 91 364 2.98e-75 234
MS.gene44883.t1 MTR_3g034130 48.561 278 123 7 1 264 91 362 7.06e-73 228
MS.gene44883.t1 MTR_4g117850 49.442 269 125 5 10 271 109 373 1.21e-72 228
MS.gene44883.t1 MTR_4g117750 50.903 277 115 8 10 273 86 354 1.77e-72 230
MS.gene44883.t1 MTR_4g117750 49.612 129 56 2 154 273 350 478 6.61e-26 106
MS.gene44883.t1 MTR_4g023130 50.775 258 114 6 19 275 65 310 3.93e-72 224
MS.gene44883.t1 MTR_4g122850 47.163 282 135 10 1 273 83 359 2.76e-71 224
MS.gene44883.t1 MTR_4g023190 47.500 280 129 9 1 275 90 356 3.42e-68 215
MS.gene44883.t1 MTR_4g023380 45.928 307 114 11 1 279 97 379 4.79e-68 216
MS.gene44883.t1 MTR_4g021785 48.134 268 125 8 10 275 97 352 6.74e-68 214
MS.gene44883.t1 MTR_8g032390 48.000 275 128 8 10 274 102 371 1.02e-67 215
MS.gene44883.t1 MTR_4g118380 48.047 256 116 6 9 251 106 357 4.31e-67 213
MS.gene44883.t1 MTR_4g023070 46.259 294 124 10 4 275 86 367 5.12e-67 213
MS.gene44883.t1 MTR_4g117770 47.183 284 139 7 1 279 90 367 5.62e-67 213
MS.gene44883.t1 MTR_4g117810 47.183 284 139 7 1 279 90 367 5.62e-67 213
MS.gene44883.t1 MTR_4g023150 46.975 281 130 10 1 275 90 357 5.68e-67 212
MS.gene44883.t1 MTR_8g433040 46.595 279 128 8 7 279 101 364 1.16e-66 212
MS.gene44883.t1 MTR_8g032380 46.595 279 128 8 7 279 101 364 1.16e-66 212
MS.gene44883.t1 MTR_4g117780 46.479 284 141 7 1 279 90 367 1.29e-66 212
MS.gene44883.t1 MTR_4g116330 46.992 266 125 7 17 275 121 377 1.89e-66 212
MS.gene44883.t1 MTR_4g118370 49.194 248 95 7 32 273 33 255 2.52e-66 207
MS.gene44883.t1 MTR_4g023120 46.259 294 124 10 4 275 86 367 4.07e-66 211
MS.gene44883.t1 MTR_4g023200 45.714 280 135 8 1 275 90 357 1.01e-64 206
MS.gene44883.t1 MTR_4g117870 44.280 271 111 7 10 279 36 267 1.24e-61 196
MS.gene44883.t1 MTR_8g433030 52.968 219 93 6 61 274 125 338 6.22e-60 194
MS.gene44883.t1 MTR_4g023440 46.182 275 97 10 8 279 106 332 1.61e-59 192
MS.gene44883.t1 MTR_4g118400 44.195 267 118 8 7 272 58 294 2.75e-58 188
MS.gene44883.t1 MTR_7g079400 44.755 286 137 11 6 279 61 337 4.84e-58 189
MS.gene44883.t1 MTR_4g116320 52.336 214 94 5 65 273 43 253 4.65e-57 184
MS.gene44883.t1 MTR_1g055165 41.935 279 107 7 2 278 93 318 2.71e-56 184
MS.gene44883.t1 MTR_1g055175 51.020 196 89 5 88 278 8 201 1.11e-54 176
MS.gene44883.t1 MTR_8g102520 43.874 253 126 9 31 274 73 318 2.78e-54 179
MS.gene44883.t1 MTR_1g055250 49.524 210 91 6 54 252 37 242 6.20e-54 178
MS.gene44883.t1 MTR_7g079370 43.011 279 136 11 14 274 79 352 7.69e-54 179
MS.gene44883.t1 MTR_4g121580 45.318 267 106 10 10 268 105 339 1.03e-53 179
MS.gene44883.t1 MTR_6g463430 49.519 208 95 5 76 279 1 202 9.89e-50 163
MS.gene44883.t1 MTR_8g040935 46.411 209 106 4 76 279 1 208 2.40e-46 154
MS.gene44883.t1 MTR_4g116330 40.602 266 103 8 17 275 121 338 2.95e-46 158
MS.gene44883.t1 MTR_8g013010 49.724 181 81 5 1 178 136 309 6.55e-46 157
MS.gene44883.t1 MTR_4g117530 38.828 273 114 9 6 273 24 248 3.28e-41 143
MS.gene44883.t1 MTR_4g117430 49.686 159 71 3 20 178 3 152 6.61e-41 139
MS.gene44883.t1 MTR_2g048580 37.809 283 135 15 1 274 88 338 1.08e-40 144
MS.gene44883.t1 MTR_7g079420 38.662 269 119 11 19 274 905 1140 3.53e-39 145
MS.gene44883.t1 MTR_4g114700 39.544 263 100 9 16 269 6 218 8.24e-38 134
MS.gene44883.t1 MTR_8g098830 45.122 164 81 4 89 247 35 194 8.51e-34 123
MS.gene44883.t1 MTR_4g118360 54.032 124 48 5 154 272 1 120 3.86e-32 115
MS.gene44883.t1 MTR_4g116340 47.887 142 58 4 13 144 112 247 2.80e-31 117
MS.gene44883.t1 MTR_7g079450 48.201 139 64 5 138 274 1 133 4.49e-30 110
MS.gene44883.t1 MTR_5g023000 59.574 94 36 2 187 279 7 99 8.01e-29 106
MS.gene44883.t1 MTR_4g122860 38.251 183 84 3 94 272 157 314 1.75e-24 100
MS.gene44883.t1 MTR_7g117720 56.075 107 39 4 175 273 166 272 2.25e-24 99.4
MS.gene44883.t1 MTR_7g117720 52.577 97 41 3 13 105 85 180 1.05e-22 94.7
MS.gene44883.t1 MTR_2g026010 31.839 223 132 9 17 220 90 311 1.28e-21 93.6
MS.gene44883.t1 MTR_1g055200 54.878 82 32 2 16 95 5 83 1.43e-20 84.7
MS.gene44883.t1 MTR_1g068835 29.384 211 130 8 13 209 87 292 2.49e-19 87.0
MS.gene44883.t1 MTR_2g031720 27.817 284 185 9 11 276 90 371 3.03e-19 87.0
MS.gene44883.t1 MTR_2g025960 29.954 217 121 8 17 208 5 215 5.89e-19 85.1
MS.gene44883.t1 MTR_6g034720 43.333 120 60 4 43 156 53 170 1.27e-18 82.4
MS.gene44883.t1 MTR_2g028140 29.787 235 120 10 17 216 107 331 1.60e-18 85.1
MS.gene44883.t1 MTR_4g070550 31.356 236 124 12 13 216 97 326 2.17e-18 84.7
MS.gene44883.t1 MTR_4g133170 28.571 210 130 8 13 208 86 289 3.59e-18 84.0
MS.gene44883.t1 MTR_2g426090 29.661 236 133 11 12 220 95 324 6.42e-18 83.2
MS.gene44883.t1 MTR_2g426070 30.435 230 127 10 17 220 105 327 1.40e-17 82.4
MS.gene44883.t1 MTR_2g031640 28.516 256 155 10 1 235 76 324 1.49e-17 82.0
MS.gene44883.t1 MTR_4g023430 54.217 83 33 3 201 279 141 222 1.72e-17 79.7
MS.gene44883.t1 MTR_1g068875 27.234 235 149 9 12 230 86 314 3.79e-17 80.9
MS.gene44883.t1 MTR_2g024110 27.907 215 135 9 11 212 88 295 4.20e-17 80.5
MS.gene44883.t1 MTR_2g028130 28.333 240 128 10 13 220 102 329 6.01e-17 80.5
MS.gene44883.t1 MTR_0667s0010 28.085 235 122 10 8 208 85 306 1.07e-16 79.7
MS.gene44883.t1 MTR_3g007410 29.317 249 144 11 2 237 135 364 1.35e-16 79.3
MS.gene44883.t1 MTR_3g007360 30.043 233 133 11 5 221 135 353 1.47e-16 79.3
MS.gene44883.t1 MTR_2g024100 29.412 221 121 9 13 204 92 306 2.15e-16 79.0
MS.gene44883.t1 MTR_1g068860 29.577 213 129 9 13 209 87 294 2.21e-16 78.6
MS.gene44883.t1 MTR_3g036290 31.000 200 113 9 14 209 116 294 3.62e-16 77.8
MS.gene44883.t1 MTR_5g011200 27.311 238 143 10 17 247 117 331 4.23e-16 77.8
MS.gene44883.t1 MTR_4g061340 28.319 226 125 7 12 216 114 323 4.86e-16 77.8
MS.gene44883.t1 MTR_4g130330 28.800 250 141 11 1 215 88 335 1.16e-15 76.6
MS.gene44883.t1 MTR_4g122840 40.000 140 38 4 146 279 165 264 1.51e-15 75.1
MS.gene44883.t1 MTR_6g080680 26.336 262 154 9 33 269 142 389 2.04e-15 75.9
MS.gene44883.t1 MTR_0054s0090 24.503 302 185 9 7 273 111 404 2.18e-15 75.9
MS.gene44883.t1 MTR_2g078870 27.749 191 116 7 19 204 59 232 5.49e-15 73.9
MS.gene44883.t1 MTR_6g090180 25.556 270 168 7 30 271 170 434 1.18e-14 73.9
MS.gene44883.t1 MTR_1g097490 26.449 276 180 11 1 270 132 390 1.37e-14 73.6
MS.gene44883.t1 MTR_3g083010 27.727 220 117 7 17 204 108 317 3.17e-14 72.4
MS.gene44883.t1 MTR_2g021050 30.131 229 120 12 13 220 91 300 3.38e-14 72.4
MS.gene44883.t1 MTR_7g079640 28.279 244 147 9 24 254 127 355 5.82e-14 71.6
MS.gene44883.t1 MTR_4g028660 23.693 287 152 11 28 271 133 395 6.62e-14 71.6
MS.gene44883.t1 MTR_4g027450 23.693 287 152 11 28 271 133 395 6.62e-14 71.6
MS.gene44883.t1 MTR_5g057790 28.638 213 125 11 17 218 142 338 6.98e-14 71.2
MS.gene44883.t1 MTR_5g096910 24.621 264 169 9 25 273 143 391 1.54e-13 70.5
MS.gene44883.t1 MTR_1g055145 50.685 73 34 2 120 192 129 199 1.67e-13 69.3
MS.gene44883.t1 MTR_2g021150 24.715 263 184 7 15 270 113 368 2.09e-13 69.7
MS.gene44883.t1 MTR_8g046200 24.830 294 171 8 24 275 137 422 2.42e-13 70.1
MS.gene44883.t1 MTR_6g021880 27.679 224 141 9 7 220 116 328 3.10e-13 69.3
MS.gene44883.t1 MTR_0054s0100 23.958 288 165 9 26 273 133 406 5.08e-13 68.9
MS.gene44883.t1 MTR_2g031680 26.667 225 143 8 13 220 87 306 5.17e-13 68.9
MS.gene44883.t1 MTR_6g086825 25.080 311 186 13 1 273 157 458 5.59e-13 68.9
MS.gene44883.t1 MTR_6g017305 25.651 269 171 10 30 270 14 281 5.68e-13 67.8
MS.gene44883.t1 MTR_7g017040 25.597 293 175 13 12 271 112 394 5.69e-13 68.6
MS.gene44883.t1 MTR_5g069120 43.158 95 43 3 14 97 83 177 5.98e-13 66.2
MS.gene44883.t1 MTR_3g009240 27.895 190 122 5 25 210 140 318 9.65e-13 68.2
MS.gene44883.t1 MTR_2g055970 28.788 198 131 5 15 208 305 496 1.05e-12 68.2
MS.gene44883.t1 MTR_0128s0020 27.727 220 129 10 15 215 118 326 1.20e-12 67.8
MS.gene44883.t1 MTR_8g013005 64.706 51 17 1 224 273 4 54 1.35e-12 62.0
MS.gene44883.t1 MTR_8g099460 27.679 224 133 9 8 208 84 301 1.60e-12 67.4
MS.gene44883.t1 MTR_4g030830 24.542 273 151 10 17 248 112 370 1.81e-12 67.4
MS.gene44883.t1 MTR_7g007300 25.847 236 140 10 17 246 205 411 2.45e-12 67.0
MS.gene44883.t1 MTR_5g096630 22.535 284 177 8 25 273 137 412 2.68e-12 66.6
MS.gene44883.t1 MTR_3g030250 25.279 269 168 10 13 270 133 379 2.86e-12 66.6
MS.gene44883.t1 MTR_1g094210 27.126 247 144 13 15 247 152 376 2.90e-12 66.6
MS.gene44883.t1 MTR_4g109290 53.030 66 26 2 11 76 75 135 3.11e-12 64.3
MS.gene44883.t1 MTR_3g006670 25.403 248 158 8 6 242 135 366 4.39e-12 66.2
MS.gene44883.t1 MTR_2g426020 24.904 261 153 9 13 243 96 343 4.42e-12 66.2
MS.gene44883.t1 MTR_1g094210 27.536 207 119 11 15 212 152 336 4.92e-12 65.9
MS.gene44883.t1 MTR_1g097560 28.352 261 145 14 1 246 119 352 5.13e-12 65.9
MS.gene44883.t1 MTR_5g043605 26.523 279 174 13 13 274 72 336 7.05e-12 65.5
MS.gene44883.t1 MTR_2g078900 26.562 192 119 7 19 204 134 309 7.98e-12 65.1
MS.gene44883.t1 MTR_3g010390 28.986 207 127 9 17 209 136 336 1.02e-11 65.1
MS.gene44883.t1 MTR_6g053240 25.871 201 129 7 15 204 125 316 1.15e-11 64.7
MS.gene44883.t1 MTR_3g009310 26.984 189 121 5 25 209 152 327 1.25e-11 64.7
MS.gene44883.t1 MTR_7g017130 25.180 278 157 13 31 278 142 398 1.47e-11 64.7
MS.gene44883.t1 MTR_3g014540 25.651 269 171 13 17 270 123 377 1.60e-11 64.3
MS.gene44883.t1 MTR_5g007610 26.923 286 175 13 8 270 126 400 3.00e-11 63.5
MS.gene44883.t1 MTR_2g029990 23.636 275 184 12 8 270 142 402 3.35e-11 63.5
MS.gene44883.t1 MTR_2g046920 27.523 218 139 9 1 213 109 312 3.57e-11 63.2
MS.gene44883.t1 MTR_1g058190 28.141 199 120 7 60 246 149 336 3.75e-11 63.2
MS.gene44883.t1 MTR_2g046830 30.455 220 130 13 1 213 110 313 3.80e-11 63.2
MS.gene44883.t1 MTR_4g028680 26.106 226 115 10 28 215 134 345 4.34e-11 63.2
MS.gene44883.t1 MTR_1g442850 27.004 237 135 11 6 215 87 312 4.41e-11 63.2
MS.gene44883.t1 MTR_3g036335 25.243 206 133 7 19 217 144 335 4.70e-11 63.2
MS.gene44883.t1 MTR_2g021155 28.151 238 148 11 17 241 130 357 5.25e-11 63.5
MS.gene44883.t1 MTR_2g021155 25.523 239 152 10 13 237 689 915 7.30e-11 62.8
MS.gene44883.t1 MTR_8g066440 26.389 288 173 14 17 271 121 402 6.61e-11 62.4
MS.gene44883.t1 MTR_7g017170 25.214 234 134 10 31 242 142 356 6.96e-11 62.4
MS.gene44883.t1 MTR_3g007430 27.230 213 130 11 10 208 127 328 6.96e-11 62.4
MS.gene44883.t1 MTR_6g065510 25.604 207 128 8 25 215 67 263 7.77e-11 62.0
MS.gene44883.t1 MTR_3g006500 26.809 235 136 11 13 240 121 326 7.82e-11 62.4
MS.gene44883.t1 MTR_2g021800 25.275 273 160 12 33 271 150 412 8.10e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44883.t1 AT4G12560 28.947 228 141 8 2 209 78 304 4.84e-17 80.9
MS.gene44883.t1 AT4G12560 28.947 228 141 8 2 209 78 304 4.84e-17 80.9
MS.gene44883.t1 AT4G12560 28.947 228 141 8 2 209 78 304 4.84e-17 80.9
MS.gene44883.t1 AT3G06240 27.723 202 132 7 13 207 163 357 1.46e-15 76.6
MS.gene44883.t1 AT3G23880 30.151 199 123 6 14 206 111 299 1.18e-13 70.5
MS.gene44883.t1 AT3G07870 29.032 217 122 11 12 204 116 324 5.65e-12 65.9

Find 66 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCCAGAGTGTATGATTTA+TGG 0.171088 5.2:+59307675 MS.gene44883:CDS
TATTGTTGGATTTCATTTAA+TGG 0.189014 5.2:+59307607 MS.gene44883:CDS
AAGACTCTTGTTCTTTCTTT+TGG 0.207374 5.2:+59307797 MS.gene44883:CDS
GTTATTCAAGCCTTTATCTA+TGG 0.251337 5.2:+59307267 MS.gene44883:CDS
AGAGTAGAGTCGCTATTAAA+TGG 0.261111 5.2:+59307539 MS.gene44883:CDS
TTGCCAAATGGTATAAATAA+TGG 0.282877 5.2:+59307653 MS.gene44883:CDS
TTTATAGATCTTGATATTAG+AGG 0.291130 5.2:+59307215 MS.gene44883:CDS
TCCTACTCGGACAATGAATA+TGG 0.303766 5.2:+59307938 MS.gene44883:CDS
GAATCTCTGCTTTCACTTCC+CGG 0.307927 5.2:+59307983 MS.gene44883:CDS
TATGATTTATGGGTATTTAG+AGG 0.334732 5.2:+59307686 MS.gene44883:CDS
TGGAATATGTGGCGTGATAA+AGG 0.350876 5.2:+59307722 MS.gene44883:CDS
TAGATAAAGGCTTGAATAAC+CGG 0.360813 5.2:-59307264 None:intergenic
TTCCAGAGTGTATGATTTAT+GGG 0.367996 5.2:+59307676 MS.gene44883:CDS
TTGGCCAAGTATAATGATAA+AGG 0.374960 5.2:+59307896 MS.gene44883:CDS
TTAGAGGATGTCTCAGTCTA+TGG 0.381600 5.2:+59307702 MS.gene44883:CDS
AAAATAATCTGCTTCTAAAT+CGG 0.383409 5.2:-59307342 None:intergenic
GAACCATTATTTATACCATT+TGG 0.393822 5.2:-59307656 None:intergenic
TATTAAATGGGGTTATTCAC+TGG 0.396592 5.2:+59307552 MS.gene44883:CDS
CTGTCCTTTATCATTATACT+TGG 0.401300 5.2:-59307900 None:intergenic
GAGTAGAGTCGCTATTAAAT+GGG 0.413688 5.2:+59307540 MS.gene44883:CDS
TCCCGGTGGTGACGGTGATC+AGG 0.418651 5.2:+59308000 MS.gene44883:CDS
AGGTGGTTTGTTATCTCACT+TGG 0.419341 5.2:+59307433 MS.gene44883:CDS
TATCAAGATCTATAAAATAT+CGG 0.426644 5.2:-59307208 None:intergenic
AGATCACTGAATGTTATTGT+TGG 0.432300 5.2:+59307593 MS.gene44883:CDS
TTTCGGGCAAAAGTATTGAA+TGG 0.432445 5.2:-59307828 None:intergenic
ATAAAGGTACAATTGACATA+TGG 0.438139 5.2:+59307738 MS.gene44883:CDS
TGGATGGATTCCATAGATAA+AGG 0.442360 5.2:-59307277 None:intergenic
TTCGGGCAAAAGTATTGAAT+GGG 0.443601 5.2:-59307827 None:intergenic
ATAGAATTGCCTTTGCCAAA+TGG 0.448065 5.2:+59307641 MS.gene44883:CDS
TTGGATTTCATTTAATGGAA+AGG 0.463172 5.2:+59307612 MS.gene44883:CDS
GGTGGTTTGTTATCTCACTT+GGG 0.468464 5.2:+59307434 MS.gene44883:CDS
CTTTCACTTCCCGGTGGTGA+CGG 0.473853 5.2:+59307992 MS.gene44883:CDS
AATACAATGTGCAATCGTCT+TGG 0.487961 5.2:+59307771 MS.gene44883:CDS
GGAATCATGAACAGTAGTAA+AGG 0.490153 5.2:-59307321 None:intergenic
GAATCATGAACAGTAGTAAA+GGG 0.494070 5.2:-59307320 None:intergenic
AAATGGGGTTATTCACTGGT+TGG 0.500924 5.2:+59307556 MS.gene44883:CDS
AACCTGATCACCGTCACCAC+CGG 0.504784 5.2:-59308002 None:intergenic
TAAAGGTACAATTGACATAT+GGG 0.507071 5.2:+59307739 MS.gene44883:CDS
TGGAACTGACGATAATGTAT+TGG 0.520044 5.2:+59307877 MS.gene44883:CDS
GTCTCAGTCTATGGAATATG+TGG 0.520208 5.2:+59307711 MS.gene44883:CDS
TAACACGTGGAAGGAAATTG+AGG 0.529215 5.2:+59307475 MS.gene44883:CDS
CATAGCACATTGAAAGAACT+AGG 0.548302 5.2:-59307400 None:intergenic
ACCTGATCACCGTCACCACC+GGG 0.560215 5.2:-59308001 None:intergenic
GTGGTTTGTTATCTCACTTG+GGG 0.574732 5.2:+59307435 MS.gene44883:CDS
TACCCATAAATCATACACTC+TGG 0.579461 5.2:-59307678 None:intergenic
GGGTATTTGTCTGTGAACTC+CGG 0.580241 5.2:-59307300 None:intergenic
TTATTTATACCATTTGGCAA+AGG 0.581590 5.2:-59307650 None:intergenic
ATGTGCTATGATCCGAGTGC+AGG 0.582242 5.2:+59307413 MS.gene44883:CDS
TGTCTGTGAACTCCGGTGGA+TGG 0.584561 5.2:-59307293 None:intergenic
GATATTAGAGGTGAATGTAG+AGG 0.593796 5.2:+59307227 MS.gene44883:CDS
AACACGTGGAAGGAAATTGA+GGG 0.607346 5.2:+59307476 MS.gene44883:CDS
GATAACAAACCACCTGCACT+CGG 0.609345 5.2:-59307425 None:intergenic
TCCATATTCATTGTCCGAGT+AGG 0.611628 5.2:-59307939 None:intergenic
TATCTATGGAATCCATCCAC+CGG 0.612787 5.2:+59307281 MS.gene44883:CDS
ATTGAGAGTTAACACGTGGA+AGG 0.615061 5.2:+59307466 MS.gene44883:CDS
GCTAGAGCATCACTCCTACT+CGG 0.623312 5.2:+59307925 MS.gene44883:CDS
ATTCTGTATACATAACCACT+AGG 0.630116 5.2:-59307964 None:intergenic
TGCTATGATCCGAGTGCAGG+TGG 0.637949 5.2:+59307416 MS.gene44883:CDS
TTCTGTATACATAACCACTA+GGG 0.647948 5.2:-59307963 None:intergenic
TATTTGTCTGTGAACTCCGG+TGG 0.653787 5.2:-59307297 None:intergenic
AGTAGAGTCGCTATTAAATG+GGG 0.668633 5.2:+59307541 MS.gene44883:CDS
CAATGAATATGGATCCCTAG+TGG 0.682110 5.2:+59307949 MS.gene44883:CDS
TCTCTGCTTTCACTTCCCGG+TGG 0.698305 5.2:+59307986 MS.gene44883:CDS
TCTCATTGAGAGTTAACACG+TGG 0.703925 5.2:+59307462 MS.gene44883:CDS
ACGTGGAAGGAAATTGAGGG+TGG 0.721598 5.2:+59307479 MS.gene44883:CDS
AGATCTATAAAATATCGGTG+AGG 0.738556 5.2:-59307203 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCAAGATCTATAAAATAT+CGG - chr5.2:59307211-59307230 None:intergenic 15.0%
!! AAAATAATCTGCTTCTAAAT+CGG - chr5.2:59307345-59307364 None:intergenic 20.0%
!! TATTGTTGGATTTCATTTAA+TGG + chr5.2:59307607-59307626 MS.gene44883:CDS 20.0%
!! TTTATAGATCTTGATATTAG+AGG + chr5.2:59307215-59307234 MS.gene44883:CDS 20.0%
!!! CAGATTATTTTTATGGTTTT+GGG + chr5.2:59307354-59307373 MS.gene44883:CDS 20.0%
!!! TTAGAAGCAGATTATTTTTA+TGG + chr5.2:59307347-59307366 MS.gene44883:CDS 20.0%
! GAACCATTATTTATACCATT+TGG - chr5.2:59307659-59307678 None:intergenic 25.0%
! TATGATTTATGGGTATTTAG+AGG + chr5.2:59307686-59307705 MS.gene44883:CDS 25.0%
! TTATTTATACCATTTGGCAA+AGG - chr5.2:59307653-59307672 None:intergenic 25.0%
! TTGCCAAATGGTATAAATAA+TGG + chr5.2:59307653-59307672 MS.gene44883:CDS 25.0%
! TTGGATTTCATTTAATGGAA+AGG + chr5.2:59307612-59307631 MS.gene44883:CDS 25.0%
!! ATAAAGGTACAATTGACATA+TGG + chr5.2:59307738-59307757 MS.gene44883:CDS 25.0%
!! TAAAGGTACAATTGACATAT+GGG + chr5.2:59307739-59307758 MS.gene44883:CDS 25.0%
!!! CACTTTTTGTACAATATTTC+GGG - chr5.2:59307847-59307866 None:intergenic 25.0%
!!! CCACTTTTTGTACAATATTT+CGG - chr5.2:59307848-59307867 None:intergenic 25.0%
!!! GCAGATTATTTTTATGGTTT+TGG + chr5.2:59307353-59307372 MS.gene44883:CDS 25.0%
AGATCACTGAATGTTATTGT+TGG + chr5.2:59307593-59307612 MS.gene44883:CDS 30.0%
AGATCTATAAAATATCGGTG+AGG - chr5.2:59307206-59307225 None:intergenic 30.0%
ATTCTGTATACATAACCACT+AGG - chr5.2:59307967-59307986 None:intergenic 30.0%
CCGAAATATTGTACAAAAAG+TGG + chr5.2:59307845-59307864 MS.gene44883:CDS 30.0%
CTGTCCTTTATCATTATACT+TGG - chr5.2:59307903-59307922 None:intergenic 30.0%
GAATCATGAACAGTAGTAAA+GGG - chr5.2:59307323-59307342 None:intergenic 30.0%
GTTATTCAAGCCTTTATCTA+TGG + chr5.2:59307267-59307286 MS.gene44883:CDS 30.0%
TAGATAAAGGCTTGAATAAC+CGG - chr5.2:59307267-59307286 None:intergenic 30.0%
TATTAAATGGGGTTATTCAC+TGG + chr5.2:59307552-59307571 MS.gene44883:CDS 30.0%
TTCCAGAGTGTATGATTTAT+GGG + chr5.2:59307676-59307695 MS.gene44883:CDS 30.0%
TTCTGTATACATAACCACTA+GGG - chr5.2:59307966-59307985 None:intergenic 30.0%
TTGGCCAAGTATAATGATAA+AGG + chr5.2:59307896-59307915 MS.gene44883:CDS 30.0%
! AAGACTCTTGTTCTTTCTTT+TGG + chr5.2:59307797-59307816 MS.gene44883:CDS 30.0%
AATACAATGTGCAATCGTCT+TGG + chr5.2:59307771-59307790 MS.gene44883:CDS 35.0%
ACAAAAAGTGGTGATATCGT+TGG + chr5.2:59307857-59307876 MS.gene44883:CDS 35.0%
AGAGTAGAGTCGCTATTAAA+TGG + chr5.2:59307539-59307558 MS.gene44883:CDS 35.0%
AGTAGAGTCGCTATTAAATG+GGG + chr5.2:59307541-59307560 MS.gene44883:CDS 35.0%
ATAGAATTGCCTTTGCCAAA+TGG + chr5.2:59307641-59307660 MS.gene44883:CDS 35.0%
CATAGCACATTGAAAGAACT+AGG - chr5.2:59307403-59307422 None:intergenic 35.0%
GAGTAGAGTCGCTATTAAAT+GGG + chr5.2:59307540-59307559 MS.gene44883:CDS 35.0%
GATATTAGAGGTGAATGTAG+AGG + chr5.2:59307227-59307246 MS.gene44883:CDS 35.0%
GGAATCATGAACAGTAGTAA+AGG - chr5.2:59307324-59307343 None:intergenic 35.0%
GTTCCAGAGTGTATGATTTA+TGG + chr5.2:59307675-59307694 MS.gene44883:CDS 35.0%
TACCCATAAATCATACACTC+TGG - chr5.2:59307681-59307700 None:intergenic 35.0%
TGGAACTGACGATAATGTAT+TGG + chr5.2:59307877-59307896 MS.gene44883:CDS 35.0%
! TGGATGGATTCCATAGATAA+AGG - chr5.2:59307280-59307299 None:intergenic 35.0%
!! AGAGGCTTTATACTTTTCTC+CGG + chr5.2:59307245-59307264 MS.gene44883:CDS 35.0%
!! TTCGGGCAAAAGTATTGAAT+GGG - chr5.2:59307830-59307849 None:intergenic 35.0%
!! TTTCGGGCAAAAGTATTGAA+TGG - chr5.2:59307831-59307850 None:intergenic 35.0%
AAATGGGGTTATTCACTGGT+TGG + chr5.2:59307556-59307575 MS.gene44883:CDS 40.0%
AACACGTGGAAGGAAATTGA+GGG + chr5.2:59307476-59307495 MS.gene44883:CDS 40.0%
ATTGAGAGTTAACACGTGGA+AGG + chr5.2:59307466-59307485 MS.gene44883:CDS 40.0%
CAATGAATATGGATCCCTAG+TGG + chr5.2:59307949-59307968 MS.gene44883:CDS 40.0%
GTCTCAGTCTATGGAATATG+TGG + chr5.2:59307711-59307730 MS.gene44883:CDS 40.0%
TAACACGTGGAAGGAAATTG+AGG + chr5.2:59307475-59307494 MS.gene44883:CDS 40.0%
TATCTATGGAATCCATCCAC+CGG + chr5.2:59307281-59307300 MS.gene44883:CDS 40.0%
TCCATATTCATTGTCCGAGT+AGG - chr5.2:59307942-59307961 None:intergenic 40.0%
TCCTACTCGGACAATGAATA+TGG + chr5.2:59307938-59307957 MS.gene44883:CDS 40.0%
TCTCATTGAGAGTTAACACG+TGG + chr5.2:59307462-59307481 MS.gene44883:CDS 40.0%
TGGAATATGTGGCGTGATAA+AGG + chr5.2:59307722-59307741 MS.gene44883:CDS 40.0%
TTAGAGGATGTCTCAGTCTA+TGG + chr5.2:59307702-59307721 MS.gene44883:CDS 40.0%
!! AGGTGGTTTGTTATCTCACT+TGG + chr5.2:59307433-59307452 MS.gene44883:CDS 40.0%
!! GGTGGTTTGTTATCTCACTT+GGG + chr5.2:59307434-59307453 MS.gene44883:CDS 40.0%
!! GTGGTTTGTTATCTCACTTG+GGG + chr5.2:59307435-59307454 MS.gene44883:CDS 40.0%
GAATCTCTGCTTTCACTTCC+CGG + chr5.2:59307983-59308002 MS.gene44883:CDS 45.0%
GATAACAAACCACCTGCACT+CGG - chr5.2:59307428-59307447 None:intergenic 45.0%
GGGTATTTGTCTGTGAACTC+CGG - chr5.2:59307303-59307322 None:intergenic 45.0%
TATTTGTCTGTGAACTCCGG+TGG - chr5.2:59307300-59307319 None:intergenic 45.0%
ACGTGGAAGGAAATTGAGGG+TGG + chr5.2:59307479-59307498 MS.gene44883:CDS 50.0%
ATGTGCTATGATCCGAGTGC+AGG + chr5.2:59307413-59307432 MS.gene44883:CDS 50.0%
! GCTAGAGCATCACTCCTACT+CGG + chr5.2:59307925-59307944 MS.gene44883:CDS 50.0%
AACCTGATCACCGTCACCAC+CGG - chr5.2:59308005-59308024 None:intergenic 55.0%
CTTTCACTTCCCGGTGGTGA+CGG + chr5.2:59307992-59308011 MS.gene44883:CDS 55.0%
TCTCTGCTTTCACTTCCCGG+TGG + chr5.2:59307986-59308005 MS.gene44883:CDS 55.0%
TGCTATGATCCGAGTGCAGG+TGG + chr5.2:59307416-59307435 MS.gene44883:CDS 55.0%
TGTCTGTGAACTCCGGTGGA+TGG - chr5.2:59307296-59307315 None:intergenic 55.0%
ACCTGATCACCGTCACCACC+GGG - chr5.2:59308004-59308023 None:intergenic 60.0%
!! TCCCGGTGGTGACGGTGATC+AGG + chr5.2:59308000-59308019 MS.gene44883:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 59307188 59308027 59307188 ID=MS.gene44883
chr5.2 mRNA 59307188 59308027 59307188 ID=MS.gene44883.t1;Parent=MS.gene44883
chr5.2 exon 59307188 59308027 59307188 ID=MS.gene44883.t1.exon1;Parent=MS.gene44883.t1
chr5.2 CDS 59307188 59308027 59307188 ID=cds.MS.gene44883.t1;Parent=MS.gene44883.t1
Gene Sequence

>MS.gene44883

ATGGATTTTTCATCTCCTCACCGATATTTTATAGATCTTGATATTAGAGGTGAATGTAGAGGCTTTATACTTTTCTCCGGTTATTCAAGCCTTTATCTATGGAATCCATCCACCGGAGTTCACAGACAAATACCCTTTACTACTGTTCATGATTCCGATTTAGAAGCAGATTATTTTTATGGTTTTGGGTATGATGAGTCTACTGATGATTACCTAGTTCTTTCAATGTGCTATGATCCGAGTGCAGGTGGTTTGTTATCTCACTTGGGGATTTTCTCATTGAGAGTTAACACGTGGAAGGAAATTGAGGGTGGTGACAATTTGCGTTATTCGCAGTCGTCTATTTATTCTAGAGTAGAGTCGCTATTAAATGGGGTTATTCACTGGTTGGCTTTTCGTGATGATAGATCACTGAATGTTATTGTTGGATTTCATTTAATGGAAAGGAAACTTATAGAATTGCCTTTGCCAAATGGTATAAATAATGGTTCCAGAGTGTATGATTTATGGGTATTTAGAGGATGTCTCAGTCTATGGAATATGTGGCGTGATAAAGGTACAATTGACATATGGGTGATGAAAAAATACAATGTGCAATCGTCTTGGACTAAGACTCTTGTTCTTTCTTTTGGTGACATTCCCATTCAATACTTTTGCCCGAAATATTGTACAAAAAGTGGTGATATCGTTGGAACTGACGATAATGTATTGGCCAAGTATAATGATAAAGGACAGCTGCTAGAGCATCACTCCTACTCGGACAATGAATATGGATCCCTAGTGGTTATGTATACAGAATCTCTGCTTTCACTTCCCGGTGGTGACGGTGATCAGGTTTAA
Protein sequence

>MS.gene44883.t1

MDFSSPHRYFIDLDIRGECRGFILFSGYSSLYLWNPSTGVHRQIPFTTVHDSDLEADYFYGFGYDESTDDYLVLSMCYDPSAGGLLSHLGIFSLRVNTWKEIEGGDNLRYSQSSIYSRVESLLNGVIHWLAFRDDRSLNVIVGFHLMERKLIELPLPNGINNGSRVYDLWVFRGCLSLWNMWRDKGTIDIWVMKKYNVQSSWTKTLVLSFGDIPIQYFCPKYCTKSGDIVGTDDNVLAKYNDKGQLLEHHSYSDNEYGSLVVMYTESLLSLPGGDGDQV