AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58781


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58781.t1 MTR_4g116320 65.031 163 40 6 10 160 99 256 9.04e-61 189
MS.gene58781.t1 MTR_4g116330 60.976 164 51 4 10 162 219 380 3.55e-58 186
MS.gene58781.t1 MTR_3g103020 58.642 162 56 5 10 162 219 378 3.92e-52 170
MS.gene58781.t1 MTR_4g109280 54.070 172 66 6 2 162 190 359 6.18e-50 164
MS.gene58781.t1 MTR_6g060650 54.717 159 59 5 10 159 216 370 1.82e-48 160
MS.gene58781.t1 MTR_4g117720 54.601 163 60 6 10 159 214 375 3.53e-47 157
MS.gene58781.t1 MTR_8g040935 51.829 164 68 4 10 162 45 208 2.28e-46 150
MS.gene58781.t1 MTR_4g023130 55.063 158 59 5 10 159 157 310 1.60e-45 151
MS.gene58781.t1 MTR_4g023080 55.063 158 59 5 10 159 198 351 3.17e-45 152
MS.gene58781.t1 MTR_4g117850 52.071 169 67 6 3 159 210 376 4.30e-45 152
MS.gene58781.t1 MTR_4g109270 52.469 162 66 4 10 162 205 364 2.20e-44 150
MS.gene58781.t1 MTR_4g094365 50.888 169 65 7 2 158 198 360 5.88e-44 149
MS.gene58781.t1 MTR_4g116330 50.980 153 45 1 10 162 219 341 1.66e-43 147
MS.gene58781.t1 MTR_4g021785 53.165 158 62 5 10 159 199 352 1.38e-42 145
MS.gene58781.t1 MTR_4g118380 53.472 144 49 4 7 133 215 357 3.17e-42 144
MS.gene58781.t1 MTR_4g023380 48.634 183 57 5 10 159 198 376 6.08e-42 144
MS.gene58781.t1 MTR_5g097210 52.830 159 61 6 11 160 208 361 1.02e-41 143
MS.gene58781.t1 MTR_3g034180 53.125 160 59 6 10 159 333 486 4.45e-40 141
MS.gene58781.t1 MTR_3g034130 53.846 143 52 5 10 141 219 358 5.61e-40 138
MS.gene58781.t1 MTR_4g023070 47.727 176 66 5 6 159 196 367 7.19e-40 138
MS.gene58781.t1 MTR_4g118400 48.052 154 63 4 7 155 152 293 1.12e-39 135
MS.gene58781.t1 MTR_4g023090 47.368 171 63 5 10 157 213 379 1.73e-39 137
MS.gene58781.t1 MTR_1g055195 49.686 159 65 5 11 159 322 475 1.82e-39 139
MS.gene58781.t1 MTR_3g034160 51.899 158 64 4 10 159 219 372 2.26e-38 134
MS.gene58781.t1 MTR_4g023120 46.629 178 69 5 4 159 194 367 5.50e-38 133
MS.gene58781.t1 MTR_5g070080 50.617 162 68 6 5 157 222 380 6.01e-38 133
MS.gene58781.t1 MTR_4g122850 48.428 159 67 6 10 157 205 359 1.85e-37 132
MS.gene58781.t1 MTR_4g117770 47.531 162 71 4 10 159 205 364 1.48e-36 129
MS.gene58781.t1 MTR_4g117810 47.531 162 71 4 10 159 205 364 1.48e-36 129
MS.gene58781.t1 MTR_6g463430 47.093 172 62 6 4 159 41 199 1.53e-36 125
MS.gene58781.t1 MTR_1g055175 45.342 161 76 5 11 162 44 201 5.06e-36 124
MS.gene58781.t1 MTR_4g117780 46.296 162 73 4 10 159 205 364 6.03e-36 128
MS.gene58781.t1 MTR_5g070130 47.059 153 69 5 13 155 219 369 2.07e-35 127
MS.gene58781.t1 MTR_1g055165 45.342 161 76 5 11 162 161 318 9.81e-35 124
MS.gene58781.t1 MTR_4g023440 45.098 153 48 3 9 159 211 329 3.57e-34 122
MS.gene58781.t1 MTR_1g055250 44.444 153 76 3 11 156 114 264 5.92e-34 122
MS.gene58781.t1 MTR_4g118360 55.455 110 46 2 51 160 18 124 6.12e-34 116
MS.gene58781.t1 MTR_4g023190 50.909 165 60 8 10 159 198 356 6.25e-34 122
MS.gene58781.t1 MTR_8g433030 48.780 164 66 8 6 157 180 337 1.40e-33 121
MS.gene58781.t1 MTR_1g055135 44.375 160 74 4 11 158 215 371 1.50e-33 122
MS.gene58781.t1 MTR_8g032390 48.780 164 66 8 6 157 213 370 1.64e-33 121
MS.gene58781.t1 MTR_5g023000 61.458 96 35 1 67 162 6 99 2.95e-33 113
MS.gene58781.t1 MTR_4g023150 49.697 165 62 8 10 159 199 357 3.42e-33 120
MS.gene58781.t1 MTR_5g070088 45.283 159 71 5 11 157 226 380 5.89e-33 120
MS.gene58781.t1 MTR_4g117750 56.637 113 46 2 48 159 370 480 6.93e-33 121
MS.gene58781.t1 MTR_4g117750 44.785 163 70 5 11 158 198 355 2.51e-30 114
MS.gene58781.t1 MTR_4g117870 50.794 126 53 3 39 158 141 263 8.42e-33 117
MS.gene58781.t1 MTR_4g118370 43.976 166 60 7 2 159 117 257 4.06e-32 115
MS.gene58781.t1 MTR_4g023200 49.697 165 62 8 10 159 199 357 5.83e-32 117
MS.gene58781.t1 MTR_4g117530 42.138 159 62 4 6 162 123 253 7.57e-31 112
MS.gene58781.t1 MTR_8g433040 45.860 157 65 5 10 159 218 361 1.60e-28 108
MS.gene58781.t1 MTR_8g032380 45.860 157 65 5 10 159 218 361 1.60e-28 108
MS.gene58781.t1 MTR_7g079370 44.231 156 72 7 10 155 199 349 1.19e-26 103
MS.gene58781.t1 MTR_4g114700 58.140 86 35 1 69 153 133 218 1.62e-25 98.2
MS.gene58781.t1 MTR_7g079400 41.615 161 78 6 10 157 175 332 1.17e-24 97.4
MS.gene58781.t1 MTR_8g102520 38.415 164 71 5 10 155 164 315 2.16e-24 96.7
MS.gene58781.t1 MTR_4g122860 43.333 150 58 6 12 155 185 313 2.32e-22 91.7
MS.gene58781.t1 MTR_7g117720 60.000 85 32 2 73 155 186 270 9.78e-22 88.6
MS.gene58781.t1 MTR_7g079450 42.857 133 68 4 27 155 2 130 4.37e-21 84.0
MS.gene58781.t1 MTR_7g079420 41.463 164 67 12 10 155 985 1137 1.50e-20 87.8
MS.gene58781.t1 MTR_4g121580 37.870 169 58 7 6 160 216 351 1.83e-19 84.0
MS.gene58781.t1 MTR_4g023430 50.633 79 38 1 82 159 141 219 6.11e-19 80.5
MS.gene58781.t1 MTR_8g098830 41.538 130 62 6 10 129 69 194 7.67e-18 77.4
MS.gene58781.t1 MTR_8g013005 61.290 62 20 2 102 162 1 59 1.66e-15 67.4
MS.gene58781.t1 MTR_2g048580 34.146 164 84 7 3 155 185 335 2.72e-15 72.0
MS.gene58781.t1 MTR_4g109290 60.000 55 20 1 108 162 136 188 5.41e-12 61.2
MS.gene58781.t1 MTR_4g023100 57.143 56 20 2 104 159 65 116 1.36e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAGAGCATAACTCATTT+TGG 0.118675 8.3:+9594124 MS.gene58781:CDS
TATTATTGCATTTGATTTAA+TGG 0.155060 8.3:+9593825 MS.gene58781:CDS
GCAAGTTATTTGTGGGTATT+TGG 0.161094 8.3:+9593883 MS.gene58781:CDS
TTTGATGGGTCACTCTTTAA+TGG 0.231958 8.3:+9593763 MS.gene58781:CDS
CACTTTCTGTAGAGCATATT+GGG 0.261064 8.3:-9594041 None:intergenic
GTTATTCATTGGTTGACTTT+TGG 0.268375 8.3:+9593787 MS.gene58781:CDS
ACAGAAAGTGGTGACATTAT+TGG 0.270370 8.3:+9594054 MS.gene58781:CDS
AGGAGGATATAGTTGAGATA+TGG 0.282271 8.3:+9593935 MS.gene58781:CDS
ATTATTGCATTTGATTTAAT+GGG 0.299436 8.3:+9593826 MS.gene58781:CDS
GTTCTTGTTCTTCGTGTTGA+TGG 0.307283 8.3:+9593997 MS.gene58781:CDS
TATAATATGCGTAGCTTTGA+TGG 0.312506 8.3:+9593748 MS.gene58781:CDS
TTGATGGGTCACTCTTTAAT+GGG 0.316031 8.3:+9593764 MS.gene58781:CDS
CCACTTTCTGTAGAGCATAT+TGG 0.323473 8.3:-9594042 None:intergenic
TCTTTAATGGGGTTATTCAT+TGG 0.352748 8.3:+9593776 MS.gene58781:CDS
TGTGCATTCGTCTTGGACTA+AGG 0.384447 8.3:+9593975 MS.gene58781:CDS
CACAAATAACTTGCTGGAAA+AGG 0.400503 8.3:-9593874 None:intergenic
GGAGGATATAGTTGAGATAT+GGG 0.407950 8.3:+9593936 MS.gene58781:CDS
AATACCCACAAATAACTTGC+TGG 0.410672 8.3:-9593880 None:intergenic
ATGATCATTTGATCATCAAT+TGG 0.425885 8.3:-9594159 None:intergenic
GAGTCTCTGCTCTCACTCTC+TGG 0.442999 8.3:+9594189 MS.gene58781:CDS
TTGGTGTCGTCTTCTTAGGC+TGG 0.446163 8.3:-9594222 None:intergenic
CATTTGATCATCAATTGGTC+CGG 0.475346 8.3:-9594154 None:intergenic
CCAATATGCTCTACAGAAAG+TGG 0.475457 8.3:+9594042 MS.gene58781:CDS
AATACAATGTGCATTCGTCT+TGG 0.497447 8.3:+9593968 MS.gene58781:CDS
TTCTCAGTGGATACACTAGT+AGG 0.549872 8.3:+9593911 MS.gene58781:CDS
ATAATATGCGTAGCTTTGAT+GGG 0.553129 8.3:+9593749 MS.gene58781:CDS
TGATGGGTCACTCTTTAATG+GGG 0.565924 8.3:+9593765 MS.gene58781:CDS
CAGTGGATACACTAGTAGGA+AGG 0.612659 8.3:+9593915 MS.gene58781:CDS
GGTGACATTATTGGAACAGA+TGG 0.620272 8.3:+9594063 MS.gene58781:CDS
GTATTTGGAGACTTTCTCAG+TGG 0.646304 8.3:+9593898 MS.gene58781:CDS
TTGTTCAAGTATAATGACAA+AGG 0.652471 8.3:+9594096 MS.gene58781:CDS
TGGATACACTAGTAGGAAGG+AGG 0.660643 8.3:+9593918 MS.gene58781:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTGCATTTGATTTAAT+GGG + chr8.3:9593826-9593845 MS.gene58781:CDS 15.0%
!!! TATTATTGCATTTGATTTAA+TGG + chr8.3:9593825-9593844 MS.gene58781:CDS 15.0%
! ATGATCATTTGATCATCAAT+TGG - chr8.3:9594162-9594181 None:intergenic 25.0%
! ATTGGGAAAAAGAATTTAGA+AGG - chr8.3:9594027-9594046 None:intergenic 25.0%
! TTGTTCAAGTATAATGACAA+AGG + chr8.3:9594096-9594115 MS.gene58781:CDS 25.0%
TCTTTAATGGGGTTATTCAT+TGG + chr8.3:9593776-9593795 MS.gene58781:CDS 30.0%
! TTTTCCAGCAAGTTATTTGT+GGG + chr8.3:9593876-9593895 MS.gene58781:CDS 30.0%
!! ATAATATGCGTAGCTTTGAT+GGG + chr8.3:9593749-9593768 MS.gene58781:CDS 30.0%
!! GTTATTCATTGGTTGACTTT+TGG + chr8.3:9593787-9593806 MS.gene58781:CDS 30.0%
!! TATAATATGCGTAGCTTTGA+TGG + chr8.3:9593748-9593767 MS.gene58781:CDS 30.0%
!! TCTAGAGCATAACTCATTTT+GGG + chr8.3:9594125-9594144 MS.gene58781:CDS 30.0%
!!! TTCTAGAGCATAACTCATTT+TGG + chr8.3:9594124-9594143 MS.gene58781:CDS 30.0%
AATACAATGTGCATTCGTCT+TGG + chr8.3:9593968-9593987 MS.gene58781:CDS 35.0%
ACAGAAAGTGGTGACATTAT+TGG + chr8.3:9594054-9594073 MS.gene58781:CDS 35.0%
AGGAGGATATAGTTGAGATA+TGG + chr8.3:9593935-9593954 MS.gene58781:CDS 35.0%
CACTTTCTGTAGAGCATATT+GGG - chr8.3:9594044-9594063 None:intergenic 35.0%
CATTTGATCATCAATTGGTC+CGG - chr8.3:9594157-9594176 None:intergenic 35.0%
GCAAGTTATTTGTGGGTATT+TGG + chr8.3:9593883-9593902 MS.gene58781:CDS 35.0%
GGAGGATATAGTTGAGATAT+GGG + chr8.3:9593936-9593955 MS.gene58781:CDS 35.0%
! AATACCCACAAATAACTTGC+TGG - chr8.3:9593883-9593902 None:intergenic 35.0%
! CTTTTCCAGCAAGTTATTTG+TGG + chr8.3:9593875-9593894 MS.gene58781:CDS 35.0%
! TTGATGGGTCACTCTTTAAT+GGG + chr8.3:9593764-9593783 MS.gene58781:CDS 35.0%
! TTTGATGGGTCACTCTTTAA+TGG + chr8.3:9593763-9593782 MS.gene58781:CDS 35.0%
!! CACAAATAACTTGCTGGAAA+AGG - chr8.3:9593877-9593896 None:intergenic 35.0%
!! GGGAAAGAAACTTTTAGAGA+TGG + chr8.3:9593846-9593865 MS.gene58781:CDS 35.0%
CCAATATGCTCTACAGAAAG+TGG + chr8.3:9594042-9594061 MS.gene58781:CDS 40.0%
CCACTTTCTGTAGAGCATAT+TGG - chr8.3:9594045-9594064 None:intergenic 40.0%
GGTGACATTATTGGAACAGA+TGG + chr8.3:9594063-9594082 MS.gene58781:CDS 40.0%
GTATTTGGAGACTTTCTCAG+TGG + chr8.3:9593898-9593917 MS.gene58781:CDS 40.0%
GTTCTTGTTCTTCGTGTTGA+TGG + chr8.3:9593997-9594016 MS.gene58781:CDS 40.0%
TTCTCAGTGGATACACTAGT+AGG + chr8.3:9593911-9593930 MS.gene58781:CDS 40.0%
! TGATGGGTCACTCTTTAATG+GGG + chr8.3:9593765-9593784 MS.gene58781:CDS 40.0%
!! AACTCATTTTGGGAAGGTTC+CGG + chr8.3:9594135-9594154 MS.gene58781:CDS 40.0%
!! GAGCATAACTCATTTTGGGA+AGG + chr8.3:9594129-9594148 MS.gene58781:CDS 40.0%
!! GGTTGACTTTTGGTCATGAT+CGG + chr8.3:9593797-9593816 MS.gene58781:CDS 40.0%
!! GTTGACTTTTGGTCATGATC+GGG + chr8.3:9593798-9593817 MS.gene58781:CDS 40.0%
CAGTGGATACACTAGTAGGA+AGG + chr8.3:9593915-9593934 MS.gene58781:CDS 45.0%
TGGATACACTAGTAGGAAGG+AGG + chr8.3:9593918-9593937 MS.gene58781:CDS 45.0%
TGTGCATTCGTCTTGGACTA+AGG + chr8.3:9593975-9593994 MS.gene58781:CDS 45.0%
GAGTCTCTGCTCTCACTCTC+TGG + chr8.3:9594189-9594208 MS.gene58781:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 9593742 9594230 9593742 ID=MS.gene58781
chr8.3 mRNA 9593742 9594230 9593742 ID=MS.gene58781.t1;Parent=MS.gene58781
chr8.3 exon 9593742 9594230 9593742 ID=MS.gene58781.t1.exon1;Parent=MS.gene58781.t1
chr8.3 CDS 9593742 9594230 9593742 ID=cds.MS.gene58781.t1;Parent=MS.gene58781.t1
Gene Sequence

>MS.gene58781

ATGTATTATAATATGCGTAGCTTTGATGGGTCACTCTTTAATGGGGTTATTCATTGGTTGACTTTTGGTCATGATCGGGATGCTATTATTGCATTTGATTTAATGGGAAAGAAACTTTTAGAGATGGAGATGCCTTTTCCAGCAAGTTATTTGTGGGTATTTGGAGACTTTCTCAGTGGATACACTAGTAGGAAGGAGGATATAGTTGAGATATGGGTGATGAAAGAATACAATGTGCATTCGTCTTGGACTAAGGTTCTTGTTCTTCGTGTTGATGGCATTCCTTCTAAATTCTTTTTCCCAATATGCTCTACAGAAAGTGGTGACATTATTGGAACAGATGGTGACACTACTTTGTTCAAGTATAATGACAAAGGACAGCTTCTAGAGCATAACTCATTTTGGGAAGGTTCCGGACCAATTGATGATCAAATGATCATGTATACAGAGTCTCTGCTCTCACTCTCTGGTGACAATGTGCCAGCCTAA

Protein sequence

>MS.gene58781.t1

MYYNMRSFDGSLFNGVIHWLTFGHDRDAIIAFDLMGKKLLEMEMPFPASYLWVFGDFLSGYTSRKEDIVEIWVMKEYNVHSSWTKVLVLRVDGIPSKFFFPICSTESGDIIGTDGDTTLFKYNDKGQLLEHNSFWEGSGPIDDQMIMYTESLLSLSGDNVPA