AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50305

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50305.t1 MTR_4g023080 74.333 300 65 4 1 296 62 353 1.98e-155 438
MS.gene50305.t1 MTR_4g023090 71.875 320 65 6 1 297 69 386 2.66e-153 434
MS.gene50305.t1 MTR_4g023380 70.245 326 53 6 1 297 69 379 1.74e-148 421
MS.gene50305.t1 MTR_4g021785 71.854 302 72 5 1 297 62 355 6.96e-148 419
MS.gene50305.t1 MTR_4g023440 69.435 301 48 4 1 297 72 332 7.61e-139 395
MS.gene50305.t1 MTR_4g023130 68.100 279 77 4 22 296 42 312 5.07e-129 370
MS.gene50305.t1 MTR_4g023070 62.025 316 98 5 1 296 56 369 1.49e-128 370
MS.gene50305.t1 MTR_4g023120 61.514 317 100 5 1 297 56 370 1.92e-127 368
MS.gene50305.t1 MTR_4g023150 62.333 300 100 7 3 295 65 358 3.17e-115 336
MS.gene50305.t1 MTR_4g023200 61.258 302 104 7 1 295 63 358 1.74e-113 332
MS.gene50305.t1 MTR_4g023190 61.667 300 101 8 3 295 65 357 2.74e-112 329
MS.gene50305.t1 MTR_3g103020 58.416 303 109 8 9 297 79 378 2.32e-107 317
MS.gene50305.t1 MTR_3g034180 54.717 318 115 9 3 297 178 489 3.79e-100 302
MS.gene50305.t1 MTR_3g034160 54.890 317 114 9 3 296 64 374 1.31e-98 295
MS.gene50305.t1 MTR_4g122850 56.352 307 111 11 9 297 63 364 2.31e-98 294
MS.gene50305.t1 MTR_4g094365 54.693 309 123 8 1 297 61 364 4.53e-98 293
MS.gene50305.t1 MTR_4g117720 54.754 305 114 9 12 297 79 378 1.98e-95 286
MS.gene50305.t1 MTR_4g109270 53.896 308 122 9 3 297 64 364 2.63e-94 283
MS.gene50305.t1 MTR_3g034130 53.265 291 110 7 9 278 70 355 1.37e-92 279
MS.gene50305.t1 MTR_4g117770 54.952 313 115 10 3 297 63 367 2.50e-90 273
MS.gene50305.t1 MTR_4g117810 54.952 313 115 10 3 297 63 367 2.50e-90 273
MS.gene50305.t1 MTR_4g116330 52.349 298 127 6 12 297 86 380 2.06e-89 271
MS.gene50305.t1 MTR_6g060650 50.968 310 125 9 9 296 68 372 3.84e-88 268
MS.gene50305.t1 MTR_4g117780 54.045 309 124 9 3 297 63 367 8.85e-87 264
MS.gene50305.t1 MTR_8g433040 52.492 301 116 7 10 297 78 364 4.20e-86 262
MS.gene50305.t1 MTR_8g032380 52.492 301 116 7 10 297 78 364 4.20e-86 262
MS.gene50305.t1 MTR_4g109280 52.492 301 124 8 9 297 66 359 5.38e-85 259
MS.gene50305.t1 MTR_4g117870 50.338 296 99 9 12 297 10 267 1.76e-81 247
MS.gene50305.t1 MTR_4g117850 51.656 302 120 12 9 294 85 376 1.90e-80 248
MS.gene50305.t1 MTR_5g070130 47.573 309 140 8 9 297 71 377 4.88e-78 242
MS.gene50305.t1 MTR_8g032390 51.342 298 105 12 9 292 99 370 2.62e-77 240
MS.gene50305.t1 MTR_4g118380 47.619 294 120 7 9 272 68 357 1.54e-75 235
MS.gene50305.t1 MTR_5g070080 47.040 321 134 12 9 297 70 386 7.52e-73 229
MS.gene50305.t1 MTR_5g097210 48.161 299 131 11 9 293 71 359 4.63e-71 224
MS.gene50305.t1 MTR_1g055195 45.307 309 138 11 6 297 184 478 3.03e-70 225
MS.gene50305.t1 MTR_5g070088 44.201 319 143 7 12 297 70 386 1.50e-69 221
MS.gene50305.t1 MTR_4g116320 57.339 218 76 7 89 294 43 255 1.67e-68 214
MS.gene50305.t1 MTR_1g055135 43.631 314 141 9 9 297 73 375 2.41e-68 217
MS.gene50305.t1 MTR_4g117750 47.727 308 128 11 9 296 64 358 3.34e-66 214
MS.gene50305.t1 MTR_4g117750 56.250 112 43 3 187 295 373 481 1.58e-27 111
MS.gene50305.t1 MTR_4g116330 44.295 298 112 6 12 297 86 341 1.07e-65 209
MS.gene50305.t1 MTR_8g433030 55.702 228 85 10 75 292 116 337 3.30e-65 208
MS.gene50305.t1 MTR_8g040935 54.976 211 79 6 100 297 1 208 2.72e-64 201
MS.gene50305.t1 MTR_4g121580 44.482 299 114 14 8 287 74 339 2.38e-58 192
MS.gene50305.t1 MTR_7g079370 40.193 311 150 8 9 291 48 350 3.02e-58 191
MS.gene50305.t1 MTR_7g079400 42.140 299 146 11 19 297 46 337 3.37e-57 187
MS.gene50305.t1 MTR_1g055165 40.203 296 123 9 9 297 70 318 1.19e-56 185
MS.gene50305.t1 MTR_4g118400 41.696 283 121 8 24 291 41 294 1.41e-55 182
MS.gene50305.t1 MTR_6g463430 51.675 209 83 6 100 297 1 202 3.06e-55 178
MS.gene50305.t1 MTR_8g102520 38.658 313 146 11 1 297 40 322 2.00e-50 169
MS.gene50305.t1 MTR_8g013010 50.732 205 84 7 9 201 115 314 1.12e-49 167
MS.gene50305.t1 MTR_4g117530 41.767 249 89 8 63 297 47 253 2.32e-48 162
MS.gene50305.t1 MTR_4g118370 43.825 251 101 11 58 294 33 257 1.66e-47 160
MS.gene50305.t1 MTR_4g117430 54.430 158 63 5 46 199 3 155 1.16e-44 149
MS.gene50305.t1 MTR_1g055175 44.221 199 93 7 110 296 8 200 3.21e-43 147
MS.gene50305.t1 MTR_1g055250 42.791 215 95 8 86 284 51 253 3.94e-43 150
MS.gene50305.t1 MTR_4g114700 38.868 265 93 6 42 288 5 218 9.28e-40 139
MS.gene50305.t1 MTR_7g079420 37.079 267 127 10 45 297 905 1144 3.53e-36 137
MS.gene50305.t1 MTR_2g048580 33.663 303 151 14 12 297 73 342 3.94e-35 130
MS.gene50305.t1 MTR_7g117720 52.941 136 51 5 9 131 52 187 1.12e-33 124
MS.gene50305.t1 MTR_7g117720 59.302 86 29 2 212 291 186 271 6.66e-19 84.7
MS.gene50305.t1 MTR_4g116340 47.879 165 71 5 12 161 83 247 1.91e-33 123
MS.gene50305.t1 MTR_5g023000 62.245 98 35 1 202 297 2 99 9.88e-33 116
MS.gene50305.t1 MTR_4g118360 57.265 117 40 4 184 296 15 125 1.09e-31 114
MS.gene50305.t1 MTR_4g023430 72.840 81 17 1 222 297 142 222 1.47e-31 117
MS.gene50305.t1 MTR_8g098830 38.974 195 99 8 107 291 31 215 1.32e-29 112
MS.gene50305.t1 MTR_4g122860 36.735 245 107 11 66 297 111 320 5.77e-28 110
MS.gene50305.t1 MTR_7g079450 41.096 146 74 5 155 297 1 137 1.64e-23 94.0
MS.gene50305.t1 MTR_4g023100 77.193 57 13 0 241 297 63 119 2.20e-22 90.1
MS.gene50305.t1 MTR_6g034720 45.968 124 54 5 67 177 51 174 1.45e-20 88.2
MS.gene50305.t1 MTR_2g024110 28.417 278 173 10 43 294 93 370 1.87e-20 90.5
MS.gene50305.t1 MTR_4g133170 26.459 257 163 7 15 250 62 313 2.38e-19 87.8
MS.gene50305.t1 MTR_4g070550 29.245 212 119 8 43 224 101 311 4.23e-19 87.0
MS.gene50305.t1 MTR_2g028130 27.376 263 161 9 6 241 71 330 1.17e-18 85.9
MS.gene50305.t1 MTR_1g055200 53.750 80 32 3 42 117 5 83 4.27e-18 78.6
MS.gene50305.t1 MTR_2g021150 26.408 284 192 11 18 289 90 368 4.64e-18 84.0
MS.gene50305.t1 MTR_2g028140 27.315 216 126 8 39 224 103 317 7.65e-17 80.5
MS.gene50305.t1 MTR_4g122840 42.446 139 39 6 162 297 164 264 2.00e-16 77.8
MS.gene50305.t1 MTR_2g028160 29.661 236 132 11 43 247 106 338 2.98e-16 79.0
MS.gene50305.t1 MTR_7g070880 27.407 270 128 11 41 250 114 375 7.20e-16 77.8
MS.gene50305.t1 MTR_1g097490 28.364 275 168 12 28 289 132 390 1.47e-15 76.6
MS.gene50305.t1 MTR_1g068835 24.880 209 136 6 39 229 87 292 6.24e-15 74.7
MS.gene50305.t1 MTR_2g021800 26.214 309 177 12 27 290 110 412 9.97e-15 74.3
MS.gene50305.t1 MTR_2g425980 26.460 291 174 10 43 296 98 385 1.02e-14 74.3
MS.gene50305.t1 MTR_8g013005 62.500 56 19 1 243 296 3 58 1.18e-14 67.8
MS.gene50305.t1 MTR_4g028510 25.651 269 158 9 59 292 139 400 1.57e-14 73.9
MS.gene50305.t1 MTR_8g101580 27.347 245 162 10 56 289 155 394 2.14e-14 73.2
MS.gene50305.t1 MTR_2g426020 24.306 288 178 10 43 294 100 383 3.70e-14 72.4
MS.gene50305.t1 MTR_4g055060 27.869 244 138 13 10 229 93 322 4.65e-14 72.4
MS.gene50305.t1 MTR_2g026010 29.302 215 124 9 39 228 86 297 1.01e-13 71.2
MS.gene50305.t1 MTR_1g097330 27.037 270 175 13 8 262 74 336 1.35e-13 70.9
MS.gene50305.t1 MTR_2g021155 27.039 233 152 9 43 262 130 357 3.30e-13 70.5
MS.gene50305.t1 MTR_5g057790 30.196 255 147 14 1 238 98 338 3.89e-13 69.7
MS.gene50305.t1 MTR_4g109290 62.500 56 19 1 244 297 133 188 5.87e-13 66.6
MS.gene50305.t1 MTR_2g046920 26.293 232 156 9 9 233 89 312 8.36e-13 68.6
MS.gene50305.t1 MTR_7g070440 29.817 218 128 10 40 233 94 310 9.11e-13 68.2
MS.gene50305.t1 MTR_6g053260 26.977 215 132 10 41 242 434 636 9.38e-13 68.9
MS.gene50305.t1 MTR_5g069120 39.806 103 53 1 9 102 52 154 9.79e-13 65.9
MS.gene50305.t1 MTR_7g062060 26.502 283 170 12 40 290 124 400 1.40e-12 67.8
MS.gene50305.t1 MTR_5g011200 25.862 232 155 9 4 226 80 303 1.43e-12 67.8
MS.gene50305.t1 MTR_2g021050 25.926 270 150 13 28 266 77 327 1.76e-12 67.4
MS.gene50305.t1 MTR_3g013740 26.394 269 165 10 43 289 149 406 1.80e-12 67.8
MS.gene50305.t1 MTR_7g078960 28.351 194 119 6 59 238 22 209 1.80e-12 66.6
MS.gene50305.t1 MTR_4g061340 26.573 286 165 11 30 289 109 375 2.12e-12 67.4
MS.gene50305.t1 MTR_7g017130 26.421 299 176 14 30 295 111 398 2.55e-12 67.0
MS.gene50305.t1 MTR_3g010390 28.218 202 131 5 43 230 136 337 2.61e-12 67.0
MS.gene50305.t1 MTR_3g009300 25.856 263 165 13 32 273 86 339 2.79e-12 66.6
MS.gene50305.t1 MTR_2g046860 24.898 245 162 9 40 267 6 245 2.82e-12 66.6
MS.gene50305.t1 MTR_5g037680 25.403 248 164 8 56 289 141 381 3.08e-12 67.0
MS.gene50305.t1 MTR_6g090180 24.468 282 171 10 45 290 159 434 3.51e-12 66.6
MS.gene50305.t1 MTR_2g078870 30.000 190 109 12 44 224 58 232 3.94e-12 65.9
MS.gene50305.t1 MTR_2g426060 25.561 223 128 8 39 228 95 312 4.33e-12 66.6
MS.gene50305.t1 MTR_1g054575 29.016 193 118 11 41 223 143 326 4.44e-12 66.6
MS.gene50305.t1 MTR_3g024130 27.037 270 163 10 41 289 58 314 6.02e-12 65.5
MS.gene50305.t1 MTR_5g096710 26.045 311 174 15 26 289 97 398 6.52e-12 65.9
MS.gene50305.t1 MTR_0128s0010 25.279 269 163 9 39 273 115 379 9.95e-12 65.5
MS.gene50305.t1 MTR_3g011060 27.170 265 157 10 41 288 140 385 1.08e-11 65.1
MS.gene50305.t1 MTR_0128s0070 28.696 230 123 11 86 288 180 395 1.14e-11 65.1
MS.gene50305.t1 MTR_2g047832 24.830 294 176 12 9 267 94 377 1.15e-11 65.1
MS.gene50305.t1 MTR_7g017170 28.319 226 123 12 30 224 111 328 1.18e-11 65.1
MS.gene50305.t1 MTR_6g053240 25.991 227 132 12 41 246 125 336 1.88e-11 64.3
MS.gene50305.t1 MTR_3g009310 27.411 197 120 8 56 244 157 338 2.22e-11 64.3
MS.gene50305.t1 MTR_2g055970 25.373 268 177 11 37 289 301 560 2.52e-11 64.3
MS.gene50305.t1 MTR_7g057270 26.147 218 127 7 43 229 125 339 2.83e-11 63.9
MS.gene50305.t1 MTR_2g078900 29.592 196 115 12 38 224 128 309 2.88e-11 63.9
MS.gene50305.t1 MTR_1g055145 46.753 77 36 2 139 215 131 202 3.50e-11 63.2
MS.gene50305.t1 MTR_8g012090 26.160 237 136 9 39 262 121 331 3.57e-11 63.5
MS.gene50305.t1 MTR_7g062100 26.117 291 160 12 41 289 119 396 3.84e-11 63.5
MS.gene50305.t1 MTR_2g064960 68.293 41 11 1 258 296 18 58 3.89e-11 58.2
MS.gene50305.t1 MTR_5g022420 25.368 272 166 11 41 289 120 377 3.94e-11 63.5
MS.gene50305.t1 MTR_7g017040 25.168 298 169 17 40 295 114 399 4.11e-11 63.5
MS.gene50305.t1 MTR_7g079640 26.104 249 165 8 48 285 125 365 4.11e-11 63.5
MS.gene50305.t1 MTR_3g007360 27.106 273 160 13 43 290 145 403 4.21e-11 63.5
MS.gene50305.t1 MTR_5g097680 24.275 276 164 11 59 295 159 428 4.54e-11 63.5
MS.gene50305.t1 MTR_6g017295 25.926 270 161 11 7 243 5 268 4.93e-11 63.5
MS.gene50305.t1 MTR_2g010900 28.070 228 128 10 43 237 79 303 6.22e-11 62.8
MS.gene50305.t1 MTR_5g097330 25.090 279 174 11 39 290 129 399 6.34e-11 62.8
MS.gene50305.t1 MTR_7g055870 24.375 320 166 12 21 274 101 410 6.58e-11 62.8
MS.gene50305.t1 MTR_7g055930 24.281 313 160 12 32 274 105 410 8.12e-11 62.8
MS.gene50305.t1 MTR_3g011020 26.786 224 132 9 85 288 158 369 9.26e-11 62.4
MS.gene50305.t1 MTR_4g030830 23.684 266 157 8 38 264 107 365 9.30e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50305.t1 AT3G06240 28.390 236 147 10 39 262 163 388 3.27e-13 70.1
MS.gene50305.t1 AT3G23880 25.888 197 132 7 40 228 111 301 4.56e-13 69.3

Find 65 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCTTGCCTTTGATTTAA+TGG 0.063010 4.4:-77661588 MS.gene50305:CDS
CATTCTGGTTTGTGGGTATT+TGG 0.166612 4.4:-77661509 MS.gene50305:CDS
AGAAAAGGGTTGCTATTTAA+TGG 0.223877 4.4:-77661656 MS.gene50305:CDS
ATGACAAGGTGCTAATAATT+TGG 0.223995 4.4:+77661829 None:intergenic
TTGGCTTGGCGTCGTGATTC+AGG 0.233161 4.4:-77661620 MS.gene50305:CDS
CCTTATGGTGTCATCTCTTC+TGG 0.260970 4.4:-77661680 MS.gene50305:CDS
TTGGAGAATTTCTCAGTCTA+TGG 0.267319 4.4:-77661490 MS.gene50305:CDS
ATAATGATAAACTTGAAATA+TGG 0.273381 4.4:-77661448 MS.gene50305:CDS
ATGATAAAGGACAGCTATTA+AGG 0.278962 4.4:-77661274 MS.gene50305:CDS
TGAAACTAGGGTTGGATTAT+AGG 0.283039 4.4:+77661757 None:intergenic
TTAGCACCTTGTCATGGTTT+TGG 0.308191 4.4:-77661821 MS.gene50305:CDS
GAAAAGGGTTGCTATTTAAT+GGG 0.327715 4.4:-77661655 MS.gene50305:CDS
CACAGAGCATTAGTACATTC+TGG 0.337046 4.4:-77661524 MS.gene50305:CDS
TCAACCGGAAAATAAGATTC+TGG 0.339211 4.4:+77661962 None:intergenic
AAAGAAAATCACTTCCCTTA+TGG 0.342286 4.4:-77661695 MS.gene50305:CDS
TATTTAATGGGGTTATTCAT+TGG 0.346478 4.4:-77661643 MS.gene50305:CDS
CAACATCAGATGGACTATTA+AGG 0.361234 4.4:+77661240 None:intergenic
GTCATCTCTTCTGGCAGAAA+AGG 0.371237 4.4:-77661671 MS.gene50305:CDS
ACTTCAAATGTGTTGAAACT+AGG 0.376322 4.4:+77661744 None:intergenic
GGATTCCATATGTAGATGTT+TGG 0.378467 4.4:+77661896 None:intergenic
AAGAAATCTTAATTCTAGAT+TGG 0.392600 4.4:-77661306 MS.gene50305:CDS
TGGTGACAATGAGCAAGTTT+AGG 0.405268 4.4:-77661186 None:intergenic
CACGAACCTCTAATTTCAAC+CGG 0.420465 4.4:+77661947 None:intergenic
ATTAGTACATTCTGGTTTGT+GGG 0.428082 4.4:-77661516 MS.gene50305:CDS
CATTAGTACATTCTGGTTTG+TGG 0.428815 4.4:-77661517 MS.gene50305:CDS
AAATTAAGGTTCTGTGAAGC+AGG 0.432550 4.4:+77661992 None:intergenic
TAATGGGGTTATTCATTGGT+TGG 0.432729 4.4:-77661639 MS.gene50305:CDS
AATACAAAGTGCATTCGTAT+TGG 0.433556 4.4:-77661415 MS.gene50305:CDS
GCTAATAATTTGGAATCAAA+AGG 0.450428 4.4:+77661839 None:intergenic
TTGGTCAAGTATAATGATAA+AGG 0.451105 4.4:-77661287 MS.gene50305:CDS
GGGTTATTCATTGGTTGGCT+TGG 0.460363 4.4:-77661634 MS.gene50305:CDS
CCAGAAGAGATGACACCATA+AGG 0.461732 4.4:+77661680 None:intergenic
CTTCAAATGTGTTGAAACTA+GGG 0.465289 4.4:+77661745 None:intergenic
TAATGATAAACTTGAAATAT+GGG 0.467930 4.4:-77661447 MS.gene50305:CDS
CTTCCTTTCCATTAAATCAA+AGG 0.474732 4.4:+77661580 None:intergenic
TACATATGGAATCCATCTAC+TGG 0.475293 4.4:-77661887 MS.gene50305:CDS
CCAGTCTAGAGATGATTACT+TGG 0.477474 4.4:-77661792 MS.gene50305:CDS
AAATGTGTTGAAACTAGGGT+TGG 0.489114 4.4:+77661749 None:intergenic
TGGAGAATTTCTCAGTCTAT+GGG 0.490268 4.4:-77661489 MS.gene50305:CDS
ATTTCTCAGTCTATGGGCTA+TGG 0.507502 4.4:-77661483 MS.gene50305:CDS
GTCTAGAGATGATTACTTGG+TGG 0.513414 4.4:-77661789 MS.gene50305:CDS
AAATTAGAGGTTCGTGTAGA+GGG 0.519047 4.4:-77661940 MS.gene50305:CDS
TCATCTCTTCTGGCAGAAAA+GGG 0.524042 4.4:-77661670 MS.gene50305:CDS
GTCGTCCAAACATCTACATA+TGG 0.524920 4.4:-77661901 MS.gene50305:CDS
GAAATTAGAGGTTCGTGTAG+AGG 0.526536 4.4:-77661941 MS.gene50305:CDS
ATAGGGTGAAAGTAGCGATC+AGG 0.528097 4.4:+77661359 None:intergenic
TAAACTTGCTCATTGTCACC+AGG 0.540244 4.4:+77661188 None:intergenic
CAGAAGAGATGACACCATAA+GGG 0.544518 4.4:+77661681 None:intergenic
TCTCATTTAGAGATAATACA+TGG 0.548869 4.4:-77661718 MS.gene50305:CDS
CCAAGTAATCATCTCTAGAC+TGG 0.555163 4.4:+77661792 None:intergenic
GCTCTGTGGTCAAAATCACT+TGG 0.561634 4.4:+77661539 None:intergenic
TTGCCTTTGATTTAATGGAA+AGG 0.567973 4.4:-77661583 MS.gene50305:CDS
TGGTCAAAATCACTTGGAAG+AGG 0.569443 4.4:+77661545 None:intergenic
GAGTCTCTACTTTCACTCCC+TGG 0.572183 4.4:-77661206 MS.gene50305:CDS
AAATTATTAGCACCTTGTCA+TGG 0.574445 4.4:-77661827 MS.gene50305:CDS
AAAAGGGTTGCTATTTAATG+GGG 0.580733 4.4:-77661654 MS.gene50305:CDS
GTGAAAGTAGCGATCAGGAA+TGG 0.595805 4.4:+77661364 None:intergenic
TTGGAATCAAAAGGAAACAC+AGG 0.615428 4.4:+77661848 None:intergenic
ATCAGATGGACTATTAAGGA+AGG 0.625682 4.4:+77661244 None:intergenic
AATTAAGGTTCTGTGAAGCA+GGG 0.631097 4.4:+77661993 None:intergenic
GTATATATGACAACATCAGA+TGG 0.638194 4.4:+77661230 None:intergenic
ATATGTAGATGTTTGGACGA+CGG 0.680670 4.4:+77661903 None:intergenic
AAACTTGCTCATTGTCACCA+GGG 0.681651 4.4:+77661189 None:intergenic
TCATAACCAAAACCATGACA+AGG 0.700082 4.4:+77661815 None:intergenic
CAGAATGTACTAATGCTCTG+TGG 0.704221 4.4:+77661525 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATGATAAACTTGAAATA+TGG - chr4.4:77661797-77661816 MS.gene50305:CDS 15.0%
!! TAATGATAAACTTGAAATAT+GGG - chr4.4:77661798-77661817 MS.gene50305:CDS 15.0%
!! AAGAAATCTTAATTCTAGAT+TGG - chr4.4:77661939-77661958 MS.gene50305:CDS 20.0%
! TATTTAATGGGGTTATTCAT+TGG - chr4.4:77661602-77661621 MS.gene50305:CDS 25.0%
! TCTCATTTAGAGATAATACA+TGG - chr4.4:77661527-77661546 MS.gene50305:CDS 25.0%
! TTGGTCAAGTATAATGATAA+AGG - chr4.4:77661958-77661977 MS.gene50305:CDS 25.0%
!! GCTAATAATTTGGAATCAAA+AGG + chr4.4:77661409-77661428 None:intergenic 25.0%
!!! TGTTTTTTAAATCCAGTAGA+TGG + chr4.4:77661373-77661392 None:intergenic 25.0%
AAAGAAAATCACTTCCCTTA+TGG - chr4.4:77661550-77661569 MS.gene50305:CDS 30.0%
AAATTATTAGCACCTTGTCA+TGG - chr4.4:77661418-77661437 MS.gene50305:CDS 30.0%
AATACAAAGTGCATTCGTAT+TGG - chr4.4:77661830-77661849 MS.gene50305:CDS 30.0%
ACAAAAATCGGCGATATTAT+TGG - chr4.4:77661916-77661935 MS.gene50305:CDS 30.0%
ACTTCAAATGTGTTGAAACT+AGG + chr4.4:77661504-77661523 None:intergenic 30.0%
ATGATAAAGGACAGCTATTA+AGG - chr4.4:77661971-77661990 MS.gene50305:CDS 30.0%
CCTATACACTCTACAAAAAT+CGG - chr4.4:77661904-77661923 MS.gene50305:CDS 30.0%
CTTCAAATGTGTTGAAACTA+GGG + chr4.4:77661503-77661522 None:intergenic 30.0%
CTTCCTTTCCATTAAATCAA+AGG + chr4.4:77661668-77661687 None:intergenic 30.0%
GTATATATGACAACATCAGA+TGG + chr4.4:77662018-77662037 None:intergenic 30.0%
! ATGACAAGGTGCTAATAATT+TGG + chr4.4:77661419-77661438 None:intergenic 30.0%
! ATTCTGGAAGCGAAAAATTA+AGG + chr4.4:77661270-77661289 None:intergenic 30.0%
! CATTCTTGCCTTTGATTTAA+TGG - chr4.4:77661657-77661676 MS.gene50305:CDS 30.0%
! CGATTTTTGTAGAGTGTATA+GGG + chr4.4:77661906-77661925 None:intergenic 30.0%
! TATTTTCCGGTTGAAATTAG+AGG - chr4.4:77661292-77661311 MS.gene50305:CDS 30.0%
! TTGCCTTTGATTTAATGGAA+AGG - chr4.4:77661662-77661681 MS.gene50305:CDS 30.0%
!! AAAAGGGTTGCTATTTAATG+GGG - chr4.4:77661591-77661610 MS.gene50305:CDS 30.0%
!! AGAAAAGGGTTGCTATTTAA+TGG - chr4.4:77661589-77661608 MS.gene50305:CDS 30.0%
!! ATTAGTACATTCTGGTTTGT+GGG - chr4.4:77661729-77661748 MS.gene50305:CDS 30.0%
!! GAAAAGGGTTGCTATTTAAT+GGG - chr4.4:77661590-77661609 MS.gene50305:CDS 30.0%
AAATGTGTTGAAACTAGGGT+TGG + chr4.4:77661499-77661518 None:intergenic 35.0%
AAATTAAGGTTCTGTGAAGC+AGG + chr4.4:77661256-77661275 None:intergenic 35.0%
AAATTAGAGGTTCGTGTAGA+GGG - chr4.4:77661305-77661324 MS.gene50305:CDS 35.0%
AATTAAGGTTCTGTGAAGCA+GGG + chr4.4:77661255-77661274 None:intergenic 35.0%
ATATGTAGATGTTTGGACGA+CGG + chr4.4:77661345-77661364 None:intergenic 35.0%
GGATTCCATATGTAGATGTT+TGG + chr4.4:77661352-77661371 None:intergenic 35.0%
TAATGGGGTTATTCATTGGT+TGG - chr4.4:77661606-77661625 MS.gene50305:CDS 35.0%
TACATATGGAATCCATCTAC+TGG - chr4.4:77661358-77661377 MS.gene50305:CDS 35.0%
TCAACCGGAAAATAAGATTC+TGG + chr4.4:77661286-77661305 None:intergenic 35.0%
TCATAACCAAAACCATGACA+AGG + chr4.4:77661433-77661452 None:intergenic 35.0%
TGAAACTAGGGTTGGATTAT+AGG + chr4.4:77661491-77661510 None:intergenic 35.0%
TGGAGAATTTCTCAGTCTAT+GGG - chr4.4:77661756-77661775 MS.gene50305:CDS 35.0%
TTGGAATCAAAAGGAAACAC+AGG + chr4.4:77661400-77661419 None:intergenic 35.0%
TTGGAGAATTTCTCAGTCTA+TGG - chr4.4:77661755-77661774 MS.gene50305:CDS 35.0%
! CCGATTTTTGTAGAGTGTAT+AGG + chr4.4:77661907-77661926 None:intergenic 35.0%
!! ATCAGATGGACTATTAAGGA+AGG + chr4.4:77662004-77662023 None:intergenic 35.0%
!! CAACATCAGATGGACTATTA+AGG + chr4.4:77662008-77662027 None:intergenic 35.0%
!! CATTAGTACATTCTGGTTTG+TGG - chr4.4:77661728-77661747 MS.gene50305:CDS 35.0%
!!! GCTTCCAGAATCTTATTTTC+CGG - chr4.4:77661279-77661298 MS.gene50305:CDS 35.0%
CACGAACCTCTAATTTCAAC+CGG + chr4.4:77661301-77661320 None:intergenic 40.0%
CAGAAGAGATGACACCATAA+GGG + chr4.4:77661567-77661586 None:intergenic 40.0%
CAGAATGTACTAATGCTCTG+TGG + chr4.4:77661723-77661742 None:intergenic 40.0%
CCAAGTAATCATCTCTAGAC+TGG + chr4.4:77661456-77661475 None:intergenic 40.0%
CCAGTCTAGAGATGATTACT+TGG - chr4.4:77661453-77661472 MS.gene50305:CDS 40.0%
GAAATTAGAGGTTCGTGTAG+AGG - chr4.4:77661304-77661323 MS.gene50305:CDS 40.0%
GTCGTCCAAACATCTACATA+TGG - chr4.4:77661344-77661363 MS.gene50305:CDS 40.0%
GTCTAGAGATGATTACTTGG+TGG - chr4.4:77661456-77661475 MS.gene50305:CDS 40.0%
TCATCTCTTCTGGCAGAAAA+GGG - chr4.4:77661575-77661594 MS.gene50305:CDS 40.0%
TGGTCAAAATCACTTGGAAG+AGG + chr4.4:77661703-77661722 None:intergenic 40.0%
! AAACTTGCTCATTGTCACCA+GGG + chr4.4:77662059-77662078 None:intergenic 40.0%
! ATTTCTCAGTCTATGGGCTA+TGG - chr4.4:77661762-77661781 MS.gene50305:CDS 40.0%
! CACAGAGCATTAGTACATTC+TGG - chr4.4:77661721-77661740 MS.gene50305:CDS 40.0%
! TAAACTTGCTCATTGTCACC+AGG + chr4.4:77662060-77662079 None:intergenic 40.0%
! TTAGCACCTTGTCATGGTTT+TGG - chr4.4:77661424-77661443 MS.gene50305:CDS 40.0%
!! CATTCTGGTTTGTGGGTATT+TGG - chr4.4:77661736-77661755 MS.gene50305:CDS 40.0%
ATAGGGTGAAAGTAGCGATC+AGG + chr4.4:77661889-77661908 None:intergenic 45.0%
CCAGAAGAGATGACACCATA+AGG + chr4.4:77661568-77661587 None:intergenic 45.0%
GCTCTGTGGTCAAAATCACT+TGG + chr4.4:77661709-77661728 None:intergenic 45.0%
GTCATCTCTTCTGGCAGAAA+AGG - chr4.4:77661574-77661593 MS.gene50305:CDS 45.0%
GTGAAAGTAGCGATCAGGAA+TGG + chr4.4:77661884-77661903 None:intergenic 45.0%
! GGGTTATTCATTGGTTGGCT+TGG - chr4.4:77661611-77661630 MS.gene50305:CDS 45.0%
!! CCTTATGGTGTCATCTCTTC+TGG - chr4.4:77661565-77661584 MS.gene50305:CDS 45.0%
GAGTCTCTACTTTCACTCCC+TGG - chr4.4:77662039-77662058 MS.gene50305:CDS 50.0%
!! TTGGCTTGGCGTCGTGATTC+AGG - chr4.4:77661625-77661644 MS.gene50305:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 77661187 77662080 77661187 ID=MS.gene50305
chr4.4 mRNA 77661187 77662080 77661187 ID=MS.gene50305.t1;Parent=MS.gene50305
chr4.4 exon 77661187 77662080 77661187 ID=MS.gene50305.t1.exon1;Parent=MS.gene50305.t1
chr4.4 CDS 77661187 77662080 77661187 ID=cds.MS.gene50305.t1;Parent=MS.gene50305.t1
Gene Sequence

>MS.gene50305

ATGTGCATATCATATCTATCTCATGAAATTCAATCTATAGATTTTGAAGCATTTCTTAATGATGACCCTGCTTCACAGAACCTTAATTTTTCGCTTCCAGAATCTTATTTTCCGGTTGAAATTAGAGGTTCGTGTAGAGGGTTTATACTTTCGTACCGTCGTCCAAACATCTACATATGGAATCCATCTACTGGATTTAAAAAACAAATACCTGTGTTTCCTTTTGATTCCAAATTATTAGCACCTTGTCATGGTTTTGGTTATGACCAGTCTAGAGATGATTACTTGGTGGTTTCATTGTCCTATAATCCAACCCTAGTTTCAACACATTTGAAGTTTTTCTCATTTAGAGATAATACATGGAAAGAAAATCACTTCCCTTATGGTGTCATCTCTTCTGGCAGAAAAGGGTTGCTATTTAATGGGGTTATTCATTGGTTGGCTTGGCGTCGTGATTCAGGTTTGAATGTCATTCTTGCCTTTGATTTAATGGAAAGGAAGTTGTTTGAGATGCCTCTTCCAAGTGATTTTGACCACAGAGCATTAGTACATTCTGGTTTGTGGGTATTTGGAGAATTTCTCAGTCTATGGGCTATGGACTATGATAATGATAATGATAAACTTGAAATATGGGTGATGAAAGAATACAAAGTGCATTCGTATTGGAATAAAACTCTTGATCTTTCTGTTGATGCCATTCCTGATCGCTACTTTCACCCTATACACTCTACAAAAATCGGCGATATTATTGGAAGAAATCTTAATTCTAGATTGGTCAAGTATAATGATAAAGGACAGCTATTAAGGCATCGCTCCTTCCTTAATAGTCCATCTGATGTTGTCATATATACTGAGTCTCTACTTTCACTCCCTGGTGACAATGAGCAAGTTTAG
Protein sequence

>MS.gene50305.t1

MCISYLSHEIQSIDFEAFLNDDPASQNLNFSLPESYFPVEIRGSCRGFILSYRRPNIYIWNPSTGFKKQIPVFPFDSKLLAPCHGFGYDQSRDDYLVVSLSYNPTLVSTHLKFFSFRDNTWKENHFPYGVISSGRKGLLFNGVIHWLAWRRDSGLNVILAFDLMERKLFEMPLPSDFDHRALVHSGLWVFGEFLSLWAMDYDNDNDKLEIWVMKEYKVHSYWNKTLDLSVDAIPDRYFHPIHSTKIGDIIGRNLNSRLVKYNDKGQLLRHRSFLNSPSDVVIYTESLLSLPGDNEQV