AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88776


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88776.t1 MTR_5g070088 80.288 208 41 0 1 208 179 386 1.43e-121 350
MS.gene88776.t1 MTR_5g070130 62.791 215 71 2 1 208 165 377 1.35e-89 268
MS.gene88776.t1 MTR_5g070080 65.385 208 68 3 1 208 183 386 1.02e-88 266
MS.gene88776.t1 MTR_3g103020 51.500 200 94 2 7 204 177 375 1.28e-59 191
MS.gene88776.t1 MTR_4g117720 50.467 214 94 6 1 208 171 378 6.02e-58 187
MS.gene88776.t1 MTR_5g097210 51.707 205 91 5 1 202 159 358 1.51e-57 186
MS.gene88776.t1 MTR_6g060650 50.000 192 90 4 14 204 184 370 1.39e-55 181
MS.gene88776.t1 MTR_4g109280 49.206 189 90 4 14 200 168 352 1.47e-51 170
MS.gene88776.t1 MTR_1g055195 48.756 201 90 6 15 207 282 477 1.09e-50 171
MS.gene88776.t1 MTR_4g023080 47.692 195 89 4 14 204 166 351 1.16e-50 167
MS.gene88776.t1 MTR_4g023130 47.692 195 89 4 14 204 125 310 1.22e-50 166
MS.gene88776.t1 MTR_6g463430 44.390 205 107 2 1 204 1 199 1.44e-49 160
MS.gene88776.t1 MTR_4g116320 47.826 207 100 5 1 204 54 255 1.98e-49 161
MS.gene88776.t1 MTR_4g117750 48.585 212 94 6 1 202 148 354 3.16e-48 164
MS.gene88776.t1 MTR_4g117750 45.385 130 62 2 81 202 350 478 1.63e-23 97.4
MS.gene88776.t1 MTR_4g023090 44.286 210 97 5 14 208 182 386 6.06e-48 161
MS.gene88776.t1 MTR_4g116330 48.691 191 94 3 16 204 189 377 9.45e-48 160
MS.gene88776.t1 MTR_1g055175 46.667 195 95 6 15 204 8 198 1.29e-47 155
MS.gene88776.t1 MTR_1g055135 49.048 210 95 7 1 202 165 370 3.57e-47 159
MS.gene88776.t1 MTR_4g109270 45.178 197 96 3 14 204 171 361 4.76e-47 158
MS.gene88776.t1 MTR_1g055250 50.000 180 81 5 7 182 69 243 5.04e-47 157
MS.gene88776.t1 MTR_4g117850 46.341 205 103 5 1 200 171 373 5.76e-46 156
MS.gene88776.t1 MTR_3g034160 48.309 207 96 6 7 207 173 374 6.97e-46 155
MS.gene88776.t1 MTR_8g433030 47.573 206 98 7 1 203 140 338 1.87e-45 154
MS.gene88776.t1 MTR_4g118400 46.277 188 85 4 14 201 123 294 1.94e-45 152
MS.gene88776.t1 MTR_8g032390 47.573 206 98 7 1 203 173 371 4.48e-45 153
MS.gene88776.t1 MTR_4g118370 45.854 205 86 5 1 202 73 255 7.69e-45 150
MS.gene88776.t1 MTR_4g023120 44.335 203 97 4 14 204 169 367 4.33e-43 148
MS.gene88776.t1 MTR_3g034180 43.902 205 104 6 10 208 290 489 7.16e-43 150
MS.gene88776.t1 MTR_4g023070 42.396 217 108 4 1 204 155 367 8.09e-43 147
MS.gene88776.t1 MTR_8g040935 42.584 209 113 4 1 205 1 206 8.32e-43 143
MS.gene88776.t1 MTR_4g023150 45.918 196 95 7 14 204 168 357 2.61e-42 146
MS.gene88776.t1 MTR_4g021785 42.347 196 99 5 14 204 166 352 3.34e-42 145
MS.gene88776.t1 MTR_4g118380 48.000 175 78 4 15 180 187 357 3.34e-42 145
MS.gene88776.t1 MTR_4g023190 46.429 196 93 7 14 204 168 356 6.52e-42 145
MS.gene88776.t1 MTR_4g094365 45.789 190 98 4 14 201 172 358 9.84e-42 144
MS.gene88776.t1 MTR_3g034130 42.786 201 104 5 1 193 165 362 1.33e-41 144
MS.gene88776.t1 MTR_4g023380 41.475 217 99 5 15 208 168 379 2.52e-41 144
MS.gene88776.t1 MTR_4g023200 43.878 196 99 6 14 204 168 357 1.64e-39 139
MS.gene88776.t1 MTR_7g079370 44.271 192 99 4 16 203 165 352 1.18e-38 136
MS.gene88776.t1 MTR_7g079400 44.162 197 97 5 16 203 141 333 1.82e-38 135
MS.gene88776.t1 MTR_8g102520 42.268 194 101 6 16 203 130 318 1.96e-38 135
MS.gene88776.t1 MTR_1g055165 46.154 169 85 5 38 204 151 315 3.23e-38 134
MS.gene88776.t1 MTR_4g122850 41.748 206 111 7 1 202 159 359 3.25e-38 135
MS.gene88776.t1 MTR_4g117780 43.000 200 109 4 12 208 170 367 7.92e-37 132
MS.gene88776.t1 MTR_8g433040 43.590 195 97 6 14 208 183 364 5.25e-36 129
MS.gene88776.t1 MTR_8g032380 43.590 195 97 6 14 208 183 364 5.25e-36 129
MS.gene88776.t1 MTR_4g117770 42.640 197 110 2 14 208 172 367 2.10e-35 128
MS.gene88776.t1 MTR_4g117810 42.640 197 110 2 14 208 172 367 2.10e-35 128
MS.gene88776.t1 MTR_4g023440 40.476 210 74 8 1 208 172 332 1.62e-34 125
MS.gene88776.t1 MTR_4g117870 39.394 198 81 6 11 208 109 267 5.06e-32 117
MS.gene88776.t1 MTR_4g116330 40.838 191 70 3 16 204 189 338 6.99e-32 118
MS.gene88776.t1 MTR_7g079450 46.377 138 66 4 67 203 3 133 1.06e-30 110
MS.gene88776.t1 MTR_4g117530 38.830 188 80 4 15 202 96 248 1.70e-30 112
MS.gene88776.t1 MTR_4g118360 48.387 124 57 5 81 201 1 120 4.54e-28 102
MS.gene88776.t1 MTR_5g023000 56.842 95 39 1 108 202 2 94 9.33e-28 101
MS.gene88776.t1 MTR_2g048580 35.437 206 110 7 4 203 150 338 2.55e-26 103
MS.gene88776.t1 MTR_8g098830 40.244 164 89 5 16 175 35 193 4.00e-26 100
MS.gene88776.t1 MTR_4g122860 35.135 185 89 4 21 201 157 314 9.69e-24 96.7
MS.gene88776.t1 MTR_7g117720 58.621 87 32 2 120 202 186 272 1.86e-23 94.7
MS.gene88776.t1 MTR_7g079420 42.138 159 79 7 51 203 989 1140 1.16e-22 95.5
MS.gene88776.t1 MTR_4g121580 37.255 204 90 9 1 200 173 342 5.50e-22 92.4
MS.gene88776.t1 MTR_4g023430 53.659 82 34 2 130 208 142 222 7.00e-20 84.3
MS.gene88776.t1 MTR_8g013010 44.340 106 53 2 1 106 210 309 5.03e-17 78.2
MS.gene88776.t1 MTR_4g114700 51.136 88 40 1 114 198 131 218 1.15e-16 75.9
MS.gene88776.t1 MTR_4g117430 43.750 80 31 3 14 87 63 134 2.19e-12 62.8
MS.gene88776.t1 MTR_8g013005 58.824 51 21 0 152 202 4 54 4.43e-12 59.3
MS.gene88776.t1 MTR_4g023100 54.386 57 23 2 152 208 66 119 5.75e-12 60.8
MS.gene88776.t1 MTR_4g122840 35.606 132 48 4 73 203 165 260 1.07e-11 62.8
MS.gene88776.t1 MTR_1g055145 44.828 87 44 3 34 120 117 199 7.22e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAACATGCATAGTTTAT+TGG 0.206624 5.4:+55031797 MS.gene88776:CDS
GCCTTCCTACTCGCTGCTTT+TGG 0.288891 5.4:+55031739 MS.gene88776:CDS
AAAATACTGTCCTGCGAATA+AGG 0.289089 5.4:-55031426 None:intergenic
GAATCTCTGCTTTCAATTCC+TGG 0.293988 5.4:+55031903 MS.gene88776:CDS
TCCTACTGTGACAATGAATA+TGG 0.321509 5.4:+55031858 MS.gene88776:CDS
ATAAAGGTGCAGTTGAAATA+TGG 0.331433 5.4:+55031655 MS.gene88776:CDS
ATAAAGGATGTCTTGCTCTA+TGG 0.333566 5.4:+55031619 MS.gene88776:CDS
CTGTCCTTTGTCATTATACT+TGG 0.343254 5.4:-55031820 None:intergenic
CTAGTAGTGTGTATGAATTA+TGG 0.346010 5.4:+55031592 MS.gene88776:CDS
TGGGATCTTTCGTCTGATAA+AGG 0.394653 5.4:+55031639 MS.gene88776:CDS
ATATCAATTCGTGTTATTGT+TGG 0.407133 5.4:+55031507 MS.gene88776:CDS
TAGTAGTGTGTATGAATTAT+GGG 0.419654 5.4:+55031593 MS.gene88776:CDS
TCTCAAATGGTGCTCTTCAC+TGG 0.420308 5.4:+55031466 MS.gene88776:CDS
CATAATTCATACACACTACT+AGG 0.438164 5.4:-55031591 None:intergenic
AAATGGTGCTCTTCACTGGT+TGG 0.443938 5.4:+55031470 MS.gene88776:CDS
TATGAATTATGGGTGTATAA+AGG 0.444879 5.4:+55031603 MS.gene88776:CDS
AGAGCTCGGACTCGGTTCAT+AGG 0.446194 5.4:-55031325 None:intergenic
GGACCATACACGATATCATT+TGG 0.447942 5.4:-55031570 None:intergenic
TAAAGGATGTCTTGCTCTAT+GGG 0.453000 5.4:+55031620 MS.gene88776:CDS
TTGGCTTTCATTTAATTGAA+AGG 0.461881 5.4:+55031526 MS.gene88776:CDS
AATACAATGTGCAATCGTCT+TGG 0.487961 5.4:+55031688 MS.gene88776:CDS
TGATGGTATGCTATCTCACT+TGG 0.499072 5.4:+55031347 MS.gene88776:CDS
TTAGTGGAGTCGCTCTCAAA+TGG 0.507369 5.4:+55031453 MS.gene88776:CDS
TTGCCAAATGATATCGTGTA+TGG 0.511952 5.4:+55031567 MS.gene88776:CDS
TGTCCTGCGAATAAGGCAAA+AGG 0.515585 5.4:-55031419 None:intergenic
GAACCGAGTCCGAGCTCTGA+TGG 0.517994 5.4:+55031330 MS.gene88776:CDS
TTCTGTATACATAACCTTGA+GGG 0.520895 5.4:-55031883 None:intergenic
TAATGTGTGGAGCGCAATTG+AGG 0.536865 5.4:+55031389 MS.gene88776:CDS
TCAATTCCTGGTGGTGACAG+TGG 0.541479 5.4:+55031915 MS.gene88776:CDS
TAAAGGTGCAGTTGAAATAT+GGG 0.548466 5.4:+55031656 MS.gene88776:CDS
ATACCATCAGAGCTCGGACT+CGG 0.552731 5.4:-55031333 None:intergenic
TCCATATTCATTGTCACAGT+AGG 0.588381 5.4:-55031859 None:intergenic
TGCAGTTGAAATATGGGTGA+TGG 0.589143 5.4:+55031662 MS.gene88776:CDS
TCTCATTGAGAGCTAATGTG+TGG 0.589751 5.4:+55031376 MS.gene88776:CDS
ACTTGACCACTGTCACCACC+AGG 0.596131 5.4:-55031921 None:intergenic
GCCAAAAGCAGCGAGTAGGA+AGG 0.602964 5.4:-55031740 None:intergenic
AATGTGTGGAGCGCAATTGA+GGG 0.603196 5.4:+55031390 MS.gene88776:CDS
GATGGTATGCTATCTCACTT+GGG 0.616133 5.4:+55031348 MS.gene88776:CDS
CAATGAATATGGATCCCTCA+AGG 0.623502 5.4:+55031869 MS.gene88776:CDS
TTGGCCAAGTATAATGACAA+AGG 0.625260 5.4:+55031816 MS.gene88776:CDS
ATTCTGTATACATAACCTTG+AGG 0.635872 5.4:-55031884 None:intergenic
GATAGCATACCATCAGAGCT+CGG 0.646205 5.4:-55031339 None:intergenic
TTCGGCCAAAAGCAGCGAGT+AGG 0.672610 5.4:-55031744 None:intergenic
TCTCTGCTTTCAATTCCTGG+TGG 0.696103 5.4:+55031906 MS.gene88776:CDS
GTGTGGAGCGCAATTGAGGG+TGG 0.724694 5.4:+55031393 MS.gene88776:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACAAACATGCATAGTTTAT+TGG + chr5.4:55031797-55031816 MS.gene88776:CDS 25.0%
! TAGTAGTGTGTATGAATTAT+GGG + chr5.4:55031593-55031612 MS.gene88776:CDS 25.0%
! TATGAATTATGGGTGTATAA+AGG + chr5.4:55031603-55031622 MS.gene88776:CDS 25.0%
!! ATATCAATTCGTGTTATTGT+TGG + chr5.4:55031507-55031526 MS.gene88776:CDS 25.0%
!! TTGGCTTTCATTTAATTGAA+AGG + chr5.4:55031526-55031545 MS.gene88776:CDS 25.0%
!!! CCACTTTTTGTATAGTATTT+CGG - chr5.4:55031765-55031784 None:intergenic 25.0%
ATTCTGTATACATAACCTTG+AGG - chr5.4:55031887-55031906 None:intergenic 30.0%
CATAATTCATACACACTACT+AGG - chr5.4:55031594-55031613 None:intergenic 30.0%
CCGAAATACTATACAAAAAG+TGG + chr5.4:55031762-55031781 MS.gene88776:CDS 30.0%
CTAGTAGTGTGTATGAATTA+TGG + chr5.4:55031592-55031611 MS.gene88776:CDS 30.0%
TTCTGTATACATAACCTTGA+GGG - chr5.4:55031886-55031905 None:intergenic 30.0%
! ATAAAGGTGCAGTTGAAATA+TGG + chr5.4:55031655-55031674 MS.gene88776:CDS 30.0%
! TAAAGGTGCAGTTGAAATAT+GGG + chr5.4:55031656-55031675 MS.gene88776:CDS 30.0%
!! GGACAGTATTTTAAACTTAG+TGG + chr5.4:55031437-55031456 MS.gene88776:CDS 30.0%
AAAATACTGTCCTGCGAATA+AGG - chr5.4:55031429-55031448 None:intergenic 35.0%
AATACAATGTGCAATCGTCT+TGG + chr5.4:55031688-55031707 MS.gene88776:CDS 35.0%
ACAAAAAGTGGTGATATCGT+TGG + chr5.4:55031774-55031793 MS.gene88776:CDS 35.0%
ATAAAGGATGTCTTGCTCTA+TGG + chr5.4:55031619-55031638 MS.gene88776:CDS 35.0%
CTGTCCTTTGTCATTATACT+TGG - chr5.4:55031823-55031842 None:intergenic 35.0%
TAAAGGATGTCTTGCTCTAT+GGG + chr5.4:55031620-55031639 MS.gene88776:CDS 35.0%
TCCATATTCATTGTCACAGT+AGG - chr5.4:55031862-55031881 None:intergenic 35.0%
TCCTACTGTGACAATGAATA+TGG + chr5.4:55031858-55031877 MS.gene88776:CDS 35.0%
TTGCCAAATGATATCGTGTA+TGG + chr5.4:55031567-55031586 MS.gene88776:CDS 35.0%
TTGGCCAAGTATAATGACAA+AGG + chr5.4:55031816-55031835 MS.gene88776:CDS 35.0%
! ACTCTTGTTCTGTCTTTTGA+TGG + chr5.4:55031717-55031736 MS.gene88776:CDS 35.0%
CAATGAATATGGATCCCTCA+AGG + chr5.4:55031869-55031888 MS.gene88776:CDS 40.0%
GAATCTCTGCTTTCAATTCC+TGG + chr5.4:55031903-55031922 MS.gene88776:CDS 40.0%
GATGGTATGCTATCTCACTT+GGG + chr5.4:55031348-55031367 MS.gene88776:CDS 40.0%
GGACCATACACGATATCATT+TGG - chr5.4:55031573-55031592 None:intergenic 40.0%
TCTCATTGAGAGCTAATGTG+TGG + chr5.4:55031376-55031395 MS.gene88776:CDS 40.0%
TGATGGTATGCTATCTCACT+TGG + chr5.4:55031347-55031366 MS.gene88776:CDS 40.0%
TGCAGTTGAAATATGGGTGA+TGG + chr5.4:55031662-55031681 MS.gene88776:CDS 40.0%
TGGGATCTTTCGTCTGATAA+AGG + chr5.4:55031639-55031658 MS.gene88776:CDS 40.0%
!! TAACCTTTTGCCTTATTCGC+AGG + chr5.4:55031416-55031435 MS.gene88776:CDS 40.0%
AAATGGTGCTCTTCACTGGT+TGG + chr5.4:55031470-55031489 MS.gene88776:CDS 45.0%
AATGTGTGGAGCGCAATTGA+GGG + chr5.4:55031390-55031409 MS.gene88776:CDS 45.0%
GATAGCATACCATCAGAGCT+CGG - chr5.4:55031342-55031361 None:intergenic 45.0%
TAATGTGTGGAGCGCAATTG+AGG + chr5.4:55031389-55031408 MS.gene88776:CDS 45.0%
TCTCAAATGGTGCTCTTCAC+TGG + chr5.4:55031466-55031485 MS.gene88776:CDS 45.0%
TCTCTGCTTTCAATTCCTGG+TGG + chr5.4:55031906-55031925 MS.gene88776:CDS 45.0%
TGTCCTGCGAATAAGGCAAA+AGG - chr5.4:55031422-55031441 None:intergenic 45.0%
TTAGTGGAGTCGCTCTCAAA+TGG + chr5.4:55031453-55031472 MS.gene88776:CDS 45.0%
ATACCATCAGAGCTCGGACT+CGG - chr5.4:55031336-55031355 None:intergenic 50.0%
! TCAATTCCTGGTGGTGACAG+TGG + chr5.4:55031915-55031934 MS.gene88776:CDS 50.0%
ACTTGACCACTGTCACCACC+AGG - chr5.4:55031924-55031943 None:intergenic 55.0%
GCCAAAAGCAGCGAGTAGGA+AGG - chr5.4:55031743-55031762 None:intergenic 55.0%
TTCGGCCAAAAGCAGCGAGT+AGG - chr5.4:55031747-55031766 None:intergenic 55.0%
! AGAGCTCGGACTCGGTTCAT+AGG - chr5.4:55031328-55031347 None:intergenic 55.0%
!! GCCTTCCTACTCGCTGCTTT+TGG + chr5.4:55031739-55031758 MS.gene88776:CDS 55.0%
GTGTGGAGCGCAATTGAGGG+TGG + chr5.4:55031393-55031412 MS.gene88776:CDS 60.0%
! GAACCGAGTCCGAGCTCTGA+TGG + chr5.4:55031330-55031349 MS.gene88776:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 55031321 55031947 55031321 ID=MS.gene88776
chr5.4 mRNA 55031321 55031947 55031321 ID=MS.gene88776.t1;Parent=MS.gene88776
chr5.4 exon 55031321 55031947 55031321 ID=MS.gene88776.t1.exon1;Parent=MS.gene88776.t1
chr5.4 CDS 55031321 55031947 55031321 ID=cds.MS.gene88776.t1;Parent=MS.gene88776.t1
Gene Sequence

>MS.gene88776

ATGTCCTATGAACCGAGTCCGAGCTCTGATGGTATGCTATCTCACTTGGGAATTTTCTCATTGAGAGCTAATGTGTGGAGCGCAATTGAGGGTGGTAACCTTTTGCCTTATTCGCAGGACAGTATTTTAAACTTAGTGGAGTCGCTCTCAAATGGTGCTCTTCACTGGTTGGCTTATCGTGATGATATATCAATTCGTGTTATTGTTGGCTTTCATTTAATTGAAAGGAAACTTCTTGAGTTGCGTTTGCCAAATGATATCGTGTATGGTCCTAGTAGTGTGTATGAATTATGGGTGTATAAAGGATGTCTTGCTCTATGGGATCTTTCGTCTGATAAAGGTGCAGTTGAAATATGGGTGATGGAAAAATACAATGTGCAATCGTCTTGGACTAAGACTCTTGTTCTGTCTTTTGATGGCCTTCCTACTCGCTGCTTTTGGCCGAAATACTATACAAAAAGTGGTGATATCGTTGGAACAAACATGCATAGTTTATTGGCCAAGTATAATGACAAAGGACAGCTGCAAGAGCATCACTCCTACTGTGACAATGAATATGGATCCCTCAAGGTTATGTATACAGAATCTCTGCTTTCAATTCCTGGTGGTGACAGTGGTCAAGTTTAA

Protein sequence

>MS.gene88776.t1

MSYEPSPSSDGMLSHLGIFSLRANVWSAIEGGNLLPYSQDSILNLVESLSNGALHWLAYRDDISIRVIVGFHLIERKLLELRLPNDIVYGPSSVYELWVYKGCLALWDLSSDKGAVEIWVMEKYNVQSSWTKTLVLSFDGLPTRCFWPKYYTKSGDIVGTNMHSLLAKYNDKGQLQEHHSYCDNEYGSLKVMYTESLLSIPGGDSGQV