AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058692


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058692.t1 MTR_8g102520 64.977 217 70 1 1 211 106 322 2.34e-91 271
MS.gene058692.t1 MTR_7g079400 57.466 221 84 2 1 211 117 337 1.13e-78 239
MS.gene058692.t1 MTR_8g098830 63.529 170 55 2 22 185 32 200 1.92e-69 212
MS.gene058692.t1 MTR_7g079450 69.286 140 39 1 72 211 2 137 9.97e-66 199
MS.gene058692.t1 MTR_7g079370 52.535 217 93 3 2 209 139 354 1.82e-60 193
MS.gene058692.t1 MTR_2g048580 54.000 200 72 7 21 211 154 342 4.42e-55 179
MS.gene058692.t1 MTR_6g060650 44.749 219 101 9 2 210 164 372 3.66e-46 156
MS.gene058692.t1 MTR_7g079420 52.973 185 69 7 40 211 965 1144 4.21e-46 163
MS.gene058692.t1 MTR_4g117720 43.379 219 104 7 2 207 162 373 1.71e-43 149
MS.gene058692.t1 MTR_4g094365 43.439 221 103 9 2 211 155 364 4.41e-43 148
MS.gene058692.t1 MTR_5g070088 42.466 219 106 8 2 207 170 381 2.23e-40 142
MS.gene058692.t1 MTR_4g109280 41.743 218 110 8 4 211 149 359 2.80e-40 140
MS.gene058692.t1 MTR_4g118380 44.670 197 89 6 2 183 166 357 3.69e-40 140
MS.gene058692.t1 MTR_4g116330 43.379 219 104 8 4 211 171 380 2.32e-39 139
MS.gene058692.t1 MTR_3g103020 41.553 219 113 7 3 211 165 378 9.40e-38 134
MS.gene058692.t1 MTR_1g055175 39.196 199 111 4 21 211 5 201 1.26e-37 130
MS.gene058692.t1 MTR_8g433030 42.326 215 104 8 4 207 133 338 1.35e-37 133
MS.gene058692.t1 MTR_4g109270 41.096 219 107 8 3 211 158 364 2.88e-37 133
MS.gene058692.t1 MTR_8g032390 42.326 215 104 8 4 207 166 371 2.91e-37 133
MS.gene058692.t1 MTR_4g118370 40.000 210 107 5 2 208 64 257 3.28e-37 130
MS.gene058692.t1 MTR_8g040935 41.791 201 97 8 25 211 14 208 8.54e-37 128
MS.gene058692.t1 MTR_3g034180 38.696 230 109 9 2 210 270 488 8.35e-36 131
MS.gene058692.t1 MTR_1g055135 40.541 222 118 6 2 211 156 375 1.70e-35 129
MS.gene058692.t1 MTR_5g070130 40.724 221 112 9 2 207 156 372 3.65e-35 128
MS.gene058692.t1 MTR_5g070080 42.081 221 109 9 2 211 174 386 7.58e-35 127
MS.gene058692.t1 MTR_4g118400 43.077 195 78 8 22 205 122 294 7.88e-35 125
MS.gene058692.t1 MTR_4g117850 38.356 219 116 7 3 207 163 376 8.95e-35 127
MS.gene058692.t1 MTR_4g116320 41.014 217 104 9 3 205 46 252 1.61e-34 123
MS.gene058692.t1 MTR_1g055195 39.732 224 107 8 2 211 269 478 2.63e-34 127
MS.gene058692.t1 MTR_4g117750 39.910 223 117 6 2 210 139 358 2.83e-34 127
MS.gene058692.t1 MTR_4g117750 46.296 108 49 4 104 205 373 477 7.96e-20 87.0
MS.gene058692.t1 MTR_8g433040 40.845 213 112 7 2 211 163 364 5.21e-34 124
MS.gene058692.t1 MTR_8g032380 40.845 213 112 7 2 211 163 364 5.21e-34 124
MS.gene058692.t1 MTR_5g097210 37.089 213 122 6 2 206 150 358 1.84e-32 120
MS.gene058692.t1 MTR_3g034130 38.500 200 101 7 2 184 156 350 5.25e-32 119
MS.gene058692.t1 MTR_4g117770 39.091 220 114 7 3 211 157 367 3.81e-31 117
MS.gene058692.t1 MTR_4g117810 39.091 220 114 7 3 211 157 367 3.81e-31 117
MS.gene058692.t1 MTR_4g122850 38.739 222 117 10 2 211 150 364 7.36e-31 116
MS.gene058692.t1 MTR_4g117530 35.814 215 89 7 3 211 82 253 1.37e-30 113
MS.gene058692.t1 MTR_4g023130 39.091 220 105 8 2 210 111 312 2.41e-30 114
MS.gene058692.t1 MTR_4g116330 39.437 213 82 7 4 211 171 341 6.38e-30 113
MS.gene058692.t1 MTR_1g055250 38.028 213 117 8 2 204 56 263 8.81e-30 112
MS.gene058692.t1 MTR_4g117870 39.070 215 87 9 2 211 92 267 8.82e-30 111
MS.gene058692.t1 MTR_4g023080 39.091 220 105 8 2 210 152 353 1.13e-29 113
MS.gene058692.t1 MTR_1g055165 39.412 170 93 4 50 211 151 318 7.38e-29 110
MS.gene058692.t1 MTR_6g463430 38.071 197 104 7 24 211 15 202 7.90e-29 107
MS.gene058692.t1 MTR_4g117780 37.727 220 117 8 3 211 157 367 1.26e-28 110
MS.gene058692.t1 MTR_4g118360 43.651 126 70 1 87 211 1 126 4.20e-28 103
MS.gene058692.t1 MTR_4g021785 38.288 222 107 10 2 211 152 355 5.73e-28 108
MS.gene058692.t1 MTR_3g034160 36.323 223 120 7 4 210 158 374 1.86e-26 104
MS.gene058692.t1 MTR_4g023120 35.745 235 116 10 2 211 146 370 2.35e-26 104
MS.gene058692.t1 MTR_4g023380 35.814 215 92 8 25 205 169 371 2.64e-25 101
MS.gene058692.t1 MTR_4g023070 35.169 236 114 9 2 210 146 369 3.70e-25 101
MS.gene058692.t1 MTR_4g023090 36.726 226 106 10 4 205 166 378 3.87e-25 101
MS.gene058692.t1 MTR_4g023190 36.199 221 111 10 2 209 154 357 6.12e-25 100
MS.gene058692.t1 MTR_4g023150 36.199 221 112 10 2 209 154 358 6.83e-24 97.4
MS.gene058692.t1 MTR_5g023000 50.538 93 43 2 121 211 8 99 1.85e-23 90.5
MS.gene058692.t1 MTR_4g023440 34.742 213 95 8 1 211 162 332 7.14e-23 94.4
MS.gene058692.t1 MTR_4g023200 34.842 221 115 10 2 209 154 358 5.76e-22 92.4
MS.gene058692.t1 MTR_4g122860 34.392 189 90 7 30 210 157 319 5.86e-21 89.4
MS.gene058692.t1 MTR_4g121580 35.468 203 101 8 2 201 164 339 8.93e-20 86.7
MS.gene058692.t1 MTR_4g114700 32.524 206 85 6 4 202 60 218 1.19e-17 79.0
MS.gene058692.t1 MTR_7g117720 52.874 87 36 3 125 206 186 272 1.57e-17 79.0
MS.gene058692.t1 MTR_0128s0110 27.564 156 81 6 37 161 4 158 1.39e-11 61.6
MS.gene058692.t1 MTR_4g117430 35.897 117 55 4 2 112 49 151 3.10e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTATGATTTGATGGTATT+TGG 0.157846 7.4:+26541986 MS.gene058692:CDS
CGATGATTACATTGTAGTTT+TGG 0.211333 7.4:+26541694 MS.gene058692:CDS
TTGCTTCTGATTTGCTTTAT+AGG 0.235745 7.4:+26541804 MS.gene058692:CDS
CACAACTTGTAAAGCATATT+GGG 0.268178 7.4:-26542135 None:intergenic
CCACAACTTGTAAAGCATAT+TGG 0.287389 7.4:-26542136 None:intergenic
TCTTGAATGGTTCTATTCAT+TGG 0.308230 7.4:+26541867 MS.gene058692:CDS
TCATAGAAATTATGATTTGA+TGG 0.315173 7.4:+26541979 MS.gene058692:CDS
TTTCCTCCAGTAATATTTGA+TGG 0.321228 7.4:-26541720 None:intergenic
ATTCAAGAACGACCCGACTA+TGG 0.322369 7.4:-26541852 None:intergenic
ACTCTATATACACCGTTGTT+TGG 0.341638 7.4:-26542261 None:intergenic
CTCTATATACACCGTTGTTT+GGG 0.346270 7.4:-26542260 None:intergenic
TGTCATTTCCTTTAAATCAA+TGG 0.363707 7.4:-26541912 None:intergenic
ATAGGTTTAAAATTAAGATA+TGG 0.364548 7.4:+26542035 MS.gene058692:CDS
TGGGGTCCATCAAATATTAC+TGG 0.366116 7.4:+26541714 MS.gene058692:CDS
GAATGGTTCTATTCATTGGC+TGG 0.382596 7.4:+26541871 MS.gene058692:CDS
TCAGTGTATGGTATGTGAAT+AGG 0.396150 7.4:+26542017 MS.gene058692:CDS
TAAGCTTGCTCAGTGCCATC+AGG 0.418945 7.4:-26542298 None:intergenic
ATATCACTATCAAAATAATC+TGG 0.420762 7.4:-26541953 None:intergenic
ACAAGTTGTGGTGATATAGT+TGG 0.420850 7.4:+26542148 MS.gene058692:CDS
TAGGTTTAAAATTAAGATAT+GGG 0.433947 7.4:+26542036 MS.gene058692:CDS
CCAATATGCTTTACAAGTTG+TGG 0.444016 7.4:+26542136 MS.gene058692:CDS
GCTAATCCAACAGCAGCATC+TGG 0.462581 7.4:-26542172 None:intergenic
TTGGCGGACTTATCAGTGTA+TGG 0.462616 7.4:+26542005 MS.gene058692:CDS
TCTCATTGAGAGCTAATAAG+TGG 0.462649 7.4:+26541768 MS.gene058692:CDS
ATAGTCGGGTCGTTCTTGAA+TGG 0.471817 7.4:+26541854 MS.gene058692:CDS
CTGAAGTTGGTCCCATAGTC+GGG 0.472332 7.4:+26541840 MS.gene058692:CDS
AATACAAAGTGCATTCATCT+TGG 0.486620 7.4:+26542068 MS.gene058692:CDS
TATGATTTGATGGTATTTGG+CGG 0.504914 7.4:+26541989 MS.gene058692:CDS
ATTCTAGAGTATCACTCCTA+TGG 0.526361 7.4:+26542217 MS.gene058692:CDS
TCTGAAGTTGGTCCCATAGT+CGG 0.531749 7.4:+26541839 MS.gene058692:CDS
ATCAAATCATAATTTCTATG+AGG 0.536641 7.4:-26541977 None:intergenic
GAAAACTAGCTCGATTGAGT+TGG 0.537743 7.4:+26541739 MS.gene058692:CDS
TTCAAGAACGACCCGACTAT+GGG 0.546092 7.4:-26541851 None:intergenic
GTTGGACCAGATGCTGCTGT+TGG 0.573521 7.4:+26542166 MS.gene058692:CDS
AAGACTGCAAGCTCTGAAGT+TGG 0.574592 7.4:+26541827 MS.gene058692:CDS
TCTCTGCTTTCACTCCCTGA+TGG 0.581645 7.4:+26542283 MS.gene058692:CDS
AAGCTTGCTCAGTGCCATCA+GGG 0.607041 7.4:-26542297 None:intergenic
TTTATAGTAAGAGTCGCCAT+AGG 0.613089 7.4:-26542233 None:intergenic
TTAGCAAAGCTTAATGACAA+AGG 0.620954 7.4:+26542190 MS.gene058692:CDS
TAATAAGTGGAAACAAGTTG+AGG 0.642343 7.4:+26541781 MS.gene058692:CDS
ACAACTTGTAAAGCATATTG+GGG 0.651604 7.4:-26542134 None:intergenic
GGTCCATCAAATATTACTGG+AGG 0.665756 7.4:+26541717 MS.gene058692:CDS
CTATAAAAGATCCCAAACAA+CGG 0.697525 7.4:+26542249 MS.gene058692:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAGGTTTAAAATTAAGATA+TGG + chr7.4:26542035-26542054 MS.gene058692:CDS 15.0%
!!! TAGGTTTAAAATTAAGATAT+GGG + chr7.4:26542036-26542055 MS.gene058692:CDS 15.0%
!! ATATCACTATCAAAATAATC+TGG - chr7.4:26541956-26541975 None:intergenic 20.0%
!! ATCAAATCATAATTTCTATG+AGG - chr7.4:26541980-26541999 None:intergenic 20.0%
!! TCATAGAAATTATGATTTGA+TGG + chr7.4:26541979-26541998 MS.gene058692:CDS 20.0%
!!! AATTATGATTTGATGGTATT+TGG + chr7.4:26541986-26542005 MS.gene058692:CDS 20.0%
!!! TATTTTTGCCATTGATTTAA+AGG + chr7.4:26541904-26541923 MS.gene058692:CDS 20.0%
!! GATGATTACATTGTAGTTTT+GGG + chr7.4:26541695-26541714 MS.gene058692:CDS 25.0%
!! TGTCATTTCCTTTAAATCAA+TGG - chr7.4:26541915-26541934 None:intergenic 25.0%
!!! ATGATTACATTGTAGTTTTG+GGG + chr7.4:26541696-26541715 MS.gene058692:CDS 25.0%
AATACAAAGTGCATTCATCT+TGG + chr7.4:26542068-26542087 MS.gene058692:CDS 30.0%
ACAACTTGTAAAGCATATTG+GGG - chr7.4:26542137-26542156 None:intergenic 30.0%
CACAACTTGTAAAGCATATT+GGG - chr7.4:26542138-26542157 None:intergenic 30.0%
CTATAAAAGATCCCAAACAA+CGG + chr7.4:26542249-26542268 MS.gene058692:CDS 30.0%
TAATAAGTGGAAACAAGTTG+AGG + chr7.4:26541781-26541800 MS.gene058692:CDS 30.0%
TTAGCAAAGCTTAATGACAA+AGG + chr7.4:26542190-26542209 MS.gene058692:CDS 30.0%
TTTCCTCCAGTAATATTTGA+TGG - chr7.4:26541723-26541742 None:intergenic 30.0%
! CGATGATTACATTGTAGTTT+TGG + chr7.4:26541694-26541713 MS.gene058692:CDS 30.0%
! TATGATTTGATGGTATTTGG+CGG + chr7.4:26541989-26542008 MS.gene058692:CDS 30.0%
! TCTTGAATGGTTCTATTCAT+TGG + chr7.4:26541867-26541886 MS.gene058692:CDS 30.0%
!! TTGCTTCTGATTTGCTTTAT+AGG + chr7.4:26541804-26541823 MS.gene058692:CDS 30.0%
ACAAGTTGTGGTGATATAGT+TGG + chr7.4:26542148-26542167 MS.gene058692:CDS 35.0%
ACTCTATATACACCGTTGTT+TGG - chr7.4:26542264-26542283 None:intergenic 35.0%
CCAATATGCTTTACAAGTTG+TGG + chr7.4:26542136-26542155 MS.gene058692:CDS 35.0%
CCACAACTTGTAAAGCATAT+TGG - chr7.4:26542139-26542158 None:intergenic 35.0%
CTCTATATACACCGTTGTTT+GGG - chr7.4:26542263-26542282 None:intergenic 35.0%
TATTGGGGAAAAATTCAGAG+CGG - chr7.4:26542122-26542141 None:intergenic 35.0%
TCAGTGTATGGTATGTGAAT+AGG + chr7.4:26542017-26542036 MS.gene058692:CDS 35.0%
TCTCATTGAGAGCTAATAAG+TGG + chr7.4:26541768-26541787 MS.gene058692:CDS 35.0%
TTTATAGTAAGAGTCGCCAT+AGG - chr7.4:26542236-26542255 None:intergenic 35.0%
!! ATTCTAGAGTATCACTCCTA+TGG + chr7.4:26542217-26542236 MS.gene058692:CDS 35.0%
ATTGGGGAAAAATTCAGAGC+GGG - chr7.4:26542121-26542140 None:intergenic 40.0%
GAAAACTAGCTCGATTGAGT+TGG + chr7.4:26541739-26541758 MS.gene058692:CDS 40.0%
GGTCCATCAAATATTACTGG+AGG + chr7.4:26541717-26541736 MS.gene058692:CDS 40.0%
TGGGGTCCATCAAATATTAC+TGG + chr7.4:26541714-26541733 MS.gene058692:CDS 40.0%
! GAATGGTTCTATTCATTGGC+TGG + chr7.4:26541871-26541890 MS.gene058692:CDS 40.0%
ATAGTCGGGTCGTTCTTGAA+TGG + chr7.4:26541854-26541873 MS.gene058692:CDS 45.0%
ATTCAAGAACGACCCGACTA+TGG - chr7.4:26541855-26541874 None:intergenic 45.0%
TTCAAGAACGACCCGACTAT+GGG - chr7.4:26541854-26541873 None:intergenic 45.0%
! TCTGAAGTTGGTCCCATAGT+CGG + chr7.4:26541839-26541858 MS.gene058692:CDS 45.0%
!! AAGACTGCAAGCTCTGAAGT+TGG + chr7.4:26541827-26541846 MS.gene058692:CDS 45.0%
!! TTGGCGGACTTATCAGTGTA+TGG + chr7.4:26542005-26542024 MS.gene058692:CDS 45.0%
GCTAATCCAACAGCAGCATC+TGG - chr7.4:26542175-26542194 None:intergenic 50.0%
TCTCTGCTTTCACTCCCTGA+TGG + chr7.4:26542283-26542302 MS.gene058692:CDS 50.0%
! AAGCTTGCTCAGTGCCATCA+GGG - chr7.4:26542300-26542319 None:intergenic 50.0%
! CTGAAGTTGGTCCCATAGTC+GGG + chr7.4:26541840-26541859 MS.gene058692:CDS 50.0%
! TAAGCTTGCTCAGTGCCATC+AGG - chr7.4:26542301-26542320 None:intergenic 50.0%
GTTGGACCAGATGCTGCTGT+TGG + chr7.4:26542166-26542185 MS.gene058692:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 26541686 26542321 26541686 ID=MS.gene058692
chr7.4 mRNA 26541686 26542321 26541686 ID=MS.gene058692.t1;Parent=MS.gene058692
chr7.4 exon 26541686 26542321 26541686 ID=MS.gene058692.t1.exon1;Parent=MS.gene058692.t1
chr7.4 CDS 26541686 26542321 26541686 ID=cds.MS.gene058692.t1;Parent=MS.gene058692.t1
Gene Sequence

>MS.gene058692

ATGTCAAGCGATGATTACATTGTAGTTTTGGGGTCCATCAAATATTACTGGAGGAAAACTAGCTCGATTGAGTTGGAGATTTTCTCATTGAGAGCTAATAAGTGGAAACAAGTTGAGGTTGCTTCTGATTTGCTTTATAGGAAGACTGCAAGCTCTGAAGTTGGTCCCATAGTCGGGTCGTTCTTGAATGGTTCTATTCATTGGCTGGTTCATAATTTTATTTTTGCCATTGATTTAAAGGAAATGACAATGTCAGAGATAACTCTTCCAGATTATTTTGATAGTGATATTCCTCATAGAAATTATGATTTGATGGTATTTGGCGGACTTATCAGTGTATGGTATGTGAATAGGTTTAAAATTAAGATATGGGTGATGCAAGAATACAAAGTGCATTCATCTTGGTCTAAGACTTTTGAATTTTCTTTCTCTCCCGCTCTGAATTTTTCCCCAATATGCTTTACAAGTTGTGGTGATATAGTTGGACCAGATGCTGCTGTTGGATTAGCAAAGCTTAATGACAAAGGACAAATTCTAGAGTATCACTCCTATGGCGACTCTTACTATAAAAGATCCCAAACAACGGTGTATATAGAGTCTCTGCTTTCACTCCCTGATGGCACTGAGCAAGCTTAA

Protein sequence

>MS.gene058692.t1

MSSDDYIVVLGSIKYYWRKTSSIELEIFSLRANKWKQVEVASDLLYRKTASSEVGPIVGSFLNGSIHWLVHNFIFAIDLKEMTMSEITLPDYFDSDIPHRNYDLMVFGGLISVWYVNRFKIKIWVMQEYKVHSSWSKTFEFSFSPALNFSPICFTSCGDIVGPDAAVGLAKLNDKGQILEYHSYGDSYYKRSQTTVYIESLLSLPDGTEQA