AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene044426


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044426.t1 MTR_4g109280 69.945 183 50 3 2 182 180 359 5.07e-85 254
MS.gene044426.t1 MTR_6g060650 66.667 180 57 2 2 181 196 372 4.26e-81 245
MS.gene044426.t1 MTR_3g103020 64.516 186 58 4 2 182 196 378 2.01e-76 233
MS.gene044426.t1 MTR_4g117720 63.298 188 59 5 2 182 194 378 2.41e-73 225
MS.gene044426.t1 MTR_4g109270 61.413 184 66 3 2 182 183 364 4.69e-70 216
MS.gene044426.t1 MTR_3g034180 61.497 187 61 5 2 181 306 488 9.27e-70 219
MS.gene044426.t1 MTR_4g117850 64.324 185 57 6 1 179 195 376 2.27e-67 210
MS.gene044426.t1 MTR_3g034130 62.069 174 56 4 2 168 192 362 2.27e-67 210
MS.gene044426.t1 MTR_4g116320 60.326 184 62 6 1 179 78 255 2.90e-64 198
MS.gene044426.t1 MTR_4g118380 63.354 161 51 2 2 155 198 357 6.27e-64 201
MS.gene044426.t1 MTR_3g034160 60.428 187 63 5 2 181 192 374 5.11e-63 199
MS.gene044426.t1 MTR_4g094365 57.609 184 72 4 2 182 184 364 9.35e-62 195
MS.gene044426.t1 MTR_4g116330 58.378 185 70 4 2 182 199 380 1.53e-61 195
MS.gene044426.t1 MTR_4g023130 57.923 183 67 5 2 181 137 312 1.42e-60 191
MS.gene044426.t1 MTR_8g040935 56.383 188 74 4 1 182 23 208 9.18e-60 185
MS.gene044426.t1 MTR_4g021785 55.435 184 75 4 1 182 177 355 3.02e-59 189
MS.gene044426.t1 MTR_4g122850 56.452 186 73 6 2 182 182 364 5.99e-59 189
MS.gene044426.t1 MTR_4g023080 56.284 183 70 5 2 181 178 353 2.96e-58 186
MS.gene044426.t1 MTR_4g118400 54.857 175 64 2 1 175 134 293 5.19e-58 184
MS.gene044426.t1 MTR_8g433030 58.242 182 63 8 2 177 163 337 1.11e-55 179
MS.gene044426.t1 MTR_4g117780 54.891 184 80 2 2 182 184 367 1.11e-55 180
MS.gene044426.t1 MTR_8g032390 58.242 182 63 8 2 177 196 370 4.43e-55 178
MS.gene044426.t1 MTR_6g463430 53.005 183 78 3 2 182 26 202 1.30e-54 172
MS.gene044426.t1 MTR_4g117770 54.348 184 81 2 2 182 184 367 5.76e-54 175
MS.gene044426.t1 MTR_4g117810 54.348 184 81 2 2 182 184 367 5.76e-54 175
MS.gene044426.t1 MTR_4g023070 50.769 195 75 6 2 181 181 369 1.18e-53 175
MS.gene044426.t1 MTR_4g023090 50.254 197 76 6 2 181 194 385 2.05e-52 172
MS.gene044426.t1 MTR_1g055175 53.191 188 76 8 2 182 19 201 5.37e-52 165
MS.gene044426.t1 MTR_1g055195 56.442 163 69 1 20 182 318 478 5.76e-52 173
MS.gene044426.t1 MTR_5g097210 55.495 182 74 5 1 179 183 360 6.87e-52 170
MS.gene044426.t1 MTR_4g023380 49.758 207 72 6 2 182 179 379 8.97e-52 170
MS.gene044426.t1 MTR_4g023120 49.490 196 78 6 2 182 181 370 1.64e-51 169
MS.gene044426.t1 MTR_5g070130 51.832 191 81 5 2 182 188 377 5.15e-51 168
MS.gene044426.t1 MTR_8g433040 53.514 185 67 7 2 182 195 364 7.36e-51 167
MS.gene044426.t1 MTR_8g032380 53.514 185 67 7 2 182 195 364 7.36e-51 167
MS.gene044426.t1 MTR_4g023190 53.226 186 72 7 2 180 180 357 1.41e-50 166
MS.gene044426.t1 MTR_1g055165 56.886 167 63 6 20 182 157 318 3.05e-49 162
MS.gene044426.t1 MTR_4g118360 63.492 126 42 2 59 182 3 126 7.39e-48 152
MS.gene044426.t1 MTR_5g070080 51.630 184 83 4 2 182 206 386 9.18e-48 160
MS.gene044426.t1 MTR_4g023200 49.462 186 80 7 2 180 180 358 8.16e-46 154
MS.gene044426.t1 MTR_4g118370 47.802 182 71 5 1 179 97 257 1.18e-45 151
MS.gene044426.t1 MTR_4g023150 51.075 186 77 7 2 180 180 358 1.36e-45 153
MS.gene044426.t1 MTR_8g102520 46.486 185 93 4 2 182 140 322 1.64e-45 152
MS.gene044426.t1 MTR_4g117750 49.474 190 84 5 1 181 172 358 2.99e-45 155
MS.gene044426.t1 MTR_4g117750 60.550 109 41 1 74 180 373 481 2.51e-36 131
MS.gene044426.t1 MTR_5g070088 46.927 179 92 2 1 177 203 380 2.50e-44 151
MS.gene044426.t1 MTR_7g079400 43.915 189 96 4 2 182 151 337 3.01e-43 147
MS.gene044426.t1 MTR_1g055135 54.878 164 64 5 25 182 216 375 3.79e-43 147
MS.gene044426.t1 MTR_4g117870 46.703 182 58 5 2 182 124 267 1.59e-42 143
MS.gene044426.t1 MTR_4g116330 48.649 185 49 4 2 182 199 341 1.34e-41 142
MS.gene044426.t1 MTR_4g023440 44.199 181 60 3 2 182 193 332 1.91e-40 139
MS.gene044426.t1 MTR_7g079370 45.506 178 90 5 2 175 175 349 1.24e-38 135
MS.gene044426.t1 MTR_5g023000 65.591 93 32 0 90 182 7 99 1.34e-37 125
MS.gene044426.t1 MTR_1g055250 46.591 176 78 10 1 169 87 253 1.57e-37 132
MS.gene044426.t1 MTR_4g117530 42.541 181 70 3 2 182 107 253 2.23e-37 129
MS.gene044426.t1 MTR_8g098830 44.805 154 79 4 2 152 45 195 1.23e-36 127
MS.gene044426.t1 MTR_7g079450 47.183 142 68 3 42 182 2 137 9.76e-34 117
MS.gene044426.t1 MTR_4g122860 43.085 188 71 7 2 182 162 320 2.58e-33 121
MS.gene044426.t1 MTR_7g079420 40.107 187 97 7 5 182 964 1144 2.53e-32 122
MS.gene044426.t1 MTR_2g048580 36.364 187 101 7 2 182 168 342 2.18e-30 113
MS.gene044426.t1 MTR_4g114700 36.667 180 62 4 1 176 90 221 1.85e-29 108
MS.gene044426.t1 MTR_4g121580 41.667 180 68 8 1 175 195 342 8.26e-28 107
MS.gene044426.t1 MTR_4g117430 56.471 85 33 2 2 86 75 155 2.00e-21 85.9
MS.gene044426.t1 MTR_7g117720 51.515 99 43 3 82 175 172 270 2.81e-21 88.2
MS.gene044426.t1 MTR_4g023430 53.659 82 35 1 104 182 141 222 5.47e-21 86.3
MS.gene044426.t1 MTR_8g013010 58.537 82 27 3 2 83 235 309 6.04e-21 88.2
MS.gene044426.t1 MTR_8g013005 59.322 59 24 0 124 182 1 59 3.60e-17 72.0
MS.gene044426.t1 MTR_4g122840 40.580 138 42 5 48 182 164 264 5.90e-17 76.6
MS.gene044426.t1 MTR_4g109290 57.895 57 24 0 126 182 132 188 1.82e-14 68.6
MS.gene044426.t1 MTR_3g010970 27.624 181 109 6 2 174 225 391 7.50e-12 63.2
MS.gene044426.t1 MTR_1g055145 48.000 75 35 2 25 98 131 202 7.62e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATGACTTGTGGGTATT+TGG 0.144797 8.1:+16336572 MS.gene044426:CDS
TATTCATTAGCGTTCAAATA+AGG 0.220938 8.1:-16336392 None:intergenic
GGAGCAGGGTTCCTCTTTAA+TGG 0.253120 8.1:+16336422 MS.gene044426:CDS
GAGCAGGGTTCCTCTTTAAT+GGG 0.265091 8.1:+16336423 MS.gene044426:CDS
ACGAAGCTACAGTTGAAATA+TGG 0.272308 8.1:+16336621 MS.gene044426:CDS
ACAAAATGTGGTGATATTAT+TGG 0.310598 8.1:+16336740 MS.gene044426:CDS
ACTCTTGTCCTTCCTATTGA+TGG 0.321860 8.1:+16336683 MS.gene044426:CDS
TCTTTAATGGGGCTATTCAT+TGG 0.331439 8.1:+16336435 MS.gene044426:CDS
ACCCACAAGTCATAATAATT+AGG 0.339487 8.1:-16336566 None:intergenic
ATTCATTAGCGTTCAAATAA+GGG 0.368832 8.1:-16336391 None:intergenic
TCCACATGGATTGTTGAAAT+AGG 0.371891 8.1:-16336825 None:intergenic
TTGGCGAAGTACGATGAAAA+TGG 0.379685 8.1:+16336782 MS.gene044426:CDS
TAATGGGGCTATTCATTGGT+TGG 0.427301 8.1:+16336439 MS.gene044426:CDS
AGATAATATGTGGAAAGAGC+TGG 0.433933 8.1:+16336355 None:intergenic
ATTAAAGAGGAACCCTGCTC+CGG 0.440956 8.1:-16336420 None:intergenic
TGAGTTGGAATGCCATCAAT+AGG 0.444967 8.1:-16336695 None:intergenic
ATTGGAACAGATGGTGGTAA+TGG 0.447081 8.1:+16336758 MS.gene044426:CDS
ATCCTAATTATTATGACTTG+TGG 0.450172 8.1:+16336564 MS.gene044426:CDS
TCCTAATTATTATGACTTGT+GGG 0.453742 8.1:+16336565 MS.gene044426:CDS
CTCAGTATACATGGCCGCTC+GGG 0.457520 8.1:-16336849 None:intergenic
CGAAGCTACAGTTGAAATAT+GGG 0.462892 8.1:+16336622 MS.gene044426:CDS
AATGAATATAATCCCGGAGC+AGG 0.490513 8.1:+16336407 MS.gene044426:CDS
AGTCATAATAATTAGGATCA+TGG 0.492245 8.1:-16336559 None:intergenic
AATACAAAGTGCATTCGTCT+TGG 0.494278 8.1:+16336654 MS.gene044426:CDS
AACGCTAATGAATATAATCC+CGG 0.506207 8.1:+16336401 MS.gene044426:CDS
AATGAATAGCCCCATTAAAG+AGG 0.526680 8.1:-16336433 None:intergenic
ACTCAGTATACATGGCCGCT+CGG 0.535525 8.1:-16336850 None:intergenic
TTAAAGAGGAACCCTGCTCC+GGG 0.544713 8.1:-16336419 None:intergenic
AATGGGTAGAAGTACTGAGT+TGG 0.583626 8.1:-16336710 None:intergenic
TAATATGTGGAAAGAGCTGG+AGG 0.586191 8.1:+16336358 None:intergenic
TAGCAGAGACTCAGTATACA+TGG 0.587628 8.1:-16336858 None:intergenic
GGTGATATTATTGGAACAGA+TGG 0.588464 8.1:+16336749 MS.gene044426:CDS
GAGTCTCTGCTATCACTCCT+CGG 0.588999 8.1:+16336869 MS.gene044426:CDS
GATATTATTGGAACAGATGG+TGG 0.593108 8.1:+16336752 MS.gene044426:CDS
AACAGATGGTGGTAATGGAT+TGG 0.598103 8.1:+16336763 MS.gene044426:CDS
AGCAGGGTTCCTCTTTAATG+GGG 0.611689 8.1:+16336424 MS.gene044426:CDS
TTGGAATGCCATCAATAGGA+AGG 0.614361 8.1:-16336691 None:intergenic
TCCTATTTCAACAATCCATG+TGG 0.619417 8.1:+16336824 MS.gene044426:CDS
ATGGCCGCTCGGGATCCACA+TGG 0.650158 8.1:-16336839 None:intergenic
ATGAATATAATCCCGGAGCA+GGG 0.652117 8.1:+16336408 MS.gene044426:CDS
CCATTGTGCTGTACAAAATG+TGG 0.678555 8.1:+16336728 MS.gene044426:CDS
CAATCCATGTGGATCCCGAG+CGG 0.712280 8.1:+16336835 MS.gene044426:CDS
AAGCTTGCTCATTGTCACCG+AGG 0.712518 8.1:-16336886 None:intergenic
AATATGTGGAAAGAGCTGGA+GGG 0.747768 8.1:+16336359 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCTTTTCTATTAAATCAA+AGG - chr8.1:16336501-16336520 None:intergenic 15.0%
! ACAAAATGTGGTGATATTAT+TGG + chr8.1:16336740-16336759 MS.gene044426:CDS 25.0%
! AGTCATAATAATTAGGATCA+TGG - chr8.1:16336562-16336581 None:intergenic 25.0%
! ATCCTAATTATTATGACTTG+TGG + chr8.1:16336564-16336583 MS.gene044426:CDS 25.0%
! ATTCATTAGCGTTCAAATAA+GGG - chr8.1:16336394-16336413 None:intergenic 25.0%
! TATTCATTAGCGTTCAAATA+AGG - chr8.1:16336395-16336414 None:intergenic 25.0%
! TGGTCAAAAATAAAATCATC+CGG - chr8.1:16336542-16336561 None:intergenic 25.0%
!! ACTTTTAGATATACATTTGC+CGG + chr8.1:16336520-16336539 MS.gene044426:CDS 25.0%
!! TCCTAATTATTATGACTTGT+GGG + chr8.1:16336565-16336584 MS.gene044426:CDS 25.0%
AACGCTAATGAATATAATCC+CGG + chr8.1:16336401-16336420 MS.gene044426:CDS 30.0%
ACCCACAAGTCATAATAATT+AGG - chr8.1:16336569-16336588 None:intergenic 30.0%
! TATTATGACTTGTGGGTATT+TGG + chr8.1:16336572-16336591 MS.gene044426:CDS 30.0%
AATACAAAGTGCATTCGTCT+TGG + chr8.1:16336654-16336673 MS.gene044426:CDS 35.0%
AATGAATAGCCCCATTAAAG+AGG - chr8.1:16336436-16336455 None:intergenic 35.0%
ACGAAGCTACAGTTGAAATA+TGG + chr8.1:16336621-16336640 MS.gene044426:CDS 35.0%
CGAAGCTACAGTTGAAATAT+GGG + chr8.1:16336622-16336641 MS.gene044426:CDS 35.0%
GATATTATTGGAACAGATGG+TGG + chr8.1:16336752-16336771 MS.gene044426:CDS 35.0%
GGTGATATTATTGGAACAGA+TGG + chr8.1:16336749-16336768 MS.gene044426:CDS 35.0%
TCCTATTTCAACAATCCATG+TGG + chr8.1:16336824-16336843 MS.gene044426:CDS 35.0%
TCTTTAATGGGGCTATTCAT+TGG + chr8.1:16336435-16336454 MS.gene044426:CDS 35.0%
! CACATTTTGTACAGCACAAT+GGG - chr8.1:16336730-16336749 None:intergenic 35.0%
! TCCACATGGATTGTTGAAAT+AGG - chr8.1:16336828-16336847 None:intergenic 35.0%
! TGGAGATTTTCTCAGTCTAT+GGG + chr8.1:16336592-16336611 MS.gene044426:CDS 35.0%
! TTGGAGATTTTCTCAGTCTA+TGG + chr8.1:16336591-16336610 MS.gene044426:CDS 35.0%
AATGAATATAATCCCGGAGC+AGG + chr8.1:16336407-16336426 MS.gene044426:CDS 40.0%
ACTCTTGTCCTTCCTATTGA+TGG + chr8.1:16336683-16336702 MS.gene044426:CDS 40.0%
ATGAATATAATCCCGGAGCA+GGG + chr8.1:16336408-16336427 MS.gene044426:CDS 40.0%
CCATTGTGCTGTACAAAATG+TGG + chr8.1:16336728-16336747 MS.gene044426:CDS 40.0%
TAATGGGGCTATTCATTGGT+TGG + chr8.1:16336439-16336458 MS.gene044426:CDS 40.0%
TAGCAGAGACTCAGTATACA+TGG - chr8.1:16336861-16336880 None:intergenic 40.0%
TTGGAATGCCATCAATAGGA+AGG - chr8.1:16336694-16336713 None:intergenic 40.0%
TTGGCGAAGTACGATGAAAA+TGG + chr8.1:16336782-16336801 MS.gene044426:CDS 40.0%
! ATTGGAACAGATGGTGGTAA+TGG + chr8.1:16336758-16336777 MS.gene044426:CDS 40.0%
! CCACATTTTGTACAGCACAA+TGG - chr8.1:16336731-16336750 None:intergenic 40.0%
! TGAGTTGGAATGCCATCAAT+AGG - chr8.1:16336698-16336717 None:intergenic 40.0%
!! AACAGATGGTGGTAATGGAT+TGG + chr8.1:16336763-16336782 MS.gene044426:CDS 40.0%
!! AATGGGTAGAAGTACTGAGT+TGG - chr8.1:16336713-16336732 None:intergenic 40.0%
!! TTTTCTCAGTCTATGGGCTA+TGG + chr8.1:16336598-16336617 MS.gene044426:CDS 40.0%
AGCAGGGTTCCTCTTTAATG+GGG + chr8.1:16336424-16336443 MS.gene044426:CDS 45.0%
ATTAAAGAGGAACCCTGCTC+CGG - chr8.1:16336423-16336442 None:intergenic 45.0%
GAGCAGGGTTCCTCTTTAAT+GGG + chr8.1:16336423-16336442 MS.gene044426:CDS 45.0%
ACTCAGTATACATGGCCGCT+CGG - chr8.1:16336853-16336872 None:intergenic 50.0%
GAGTCTCTGCTATCACTCCT+CGG + chr8.1:16336869-16336888 MS.gene044426:CDS 50.0%
GGAGCAGGGTTCCTCTTTAA+TGG + chr8.1:16336422-16336441 MS.gene044426:CDS 50.0%
TTAAAGAGGAACCCTGCTCC+GGG - chr8.1:16336422-16336441 None:intergenic 50.0%
! AAGCTTGCTCATTGTCACCG+AGG - chr8.1:16336889-16336908 None:intergenic 50.0%
CAATCCATGTGGATCCCGAG+CGG + chr8.1:16336835-16336854 MS.gene044426:CDS 55.0%
CTCAGTATACATGGCCGCTC+GGG - chr8.1:16336852-16336871 None:intergenic 55.0%
ATGGCCGCTCGGGATCCACA+TGG - chr8.1:16336842-16336861 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 16336362 16336910 16336362 ID=MS.gene044426
chr8.1 mRNA 16336362 16336910 16336362 ID=MS.gene044426.t1;Parent=MS.gene044426
chr8.1 exon 16336362 16336910 16336362 ID=MS.gene044426.t1.exon1;Parent=MS.gene044426.t1
chr8.1 CDS 16336362 16336910 16336362 ID=cds.MS.gene044426.t1;Parent=MS.gene044426.t1
Gene Sequence

>MS.gene044426

ATGTGGAAAGAGCTGGAGGGTACTCACTTCCCTTATTTGAACGCTAATGAATATAATCCCGGAGCAGGGTTCCTCTTTAATGGGGCTATTCATTGGTTGGCTTTTAGTTGTGATCTACTATATGATGTTATTGTTGCCTTTGATTTAATAGAAAAGAAACTTTTAGATATACATTTGCCGGATGATTTTATTTTTGACCATGATCCTAATTATTATGACTTGTGGGTATTTGGAGATTTTCTCAGTCTATGGGCTATGGACGAAGCTACAGTTGAAATATGGGTTATGAAAGAATACAAAGTGCATTCGTCTTGGACTAAGACTCTTGTCCTTCCTATTGATGGCATTCCAACTCAGTACTTCTACCCATTGTGCTGTACAAAATGTGGTGATATTATTGGAACAGATGGTGGTAATGGATTGGCGAAGTACGATGAAAATGGAGAATTACTAGACGTTCATTCCTATTTCAACAATCCATGTGGATCCCGAGCGGCCATGTATACTGAGTCTCTGCTATCACTCCTCGGTGACAATGAGCAAGCTTAA

Protein sequence

>MS.gene044426.t1

MWKELEGTHFPYLNANEYNPGAGFLFNGAIHWLAFSCDLLYDVIVAFDLIEKKLLDIHLPDDFIFDHDPNYYDLWVFGDFLSLWAMDEATVEIWVMKEYKVHSSWTKTLVLPIDGIPTQYFYPLCCTKCGDIIGTDGGNGLAKYDENGELLDVHSYFNNPCGSRAAMYTESLLSLLGDNEQA