AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50332

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50332.t1 MTR_4g023090 64.341 258 60 5 1 243 139 379 1.26e-103 306
MS.gene50332.t1 MTR_4g023080 64.516 248 62 4 1 247 131 353 4.84e-101 298
MS.gene50332.t1 MTR_4g023130 64.516 248 62 4 1 247 90 312 1.82e-100 295
MS.gene50332.t1 MTR_4g023380 60.073 273 52 6 1 248 139 379 4.37e-99 294
MS.gene50332.t1 MTR_4g021785 61.200 250 70 4 1 248 131 355 6.83e-95 282
MS.gene50332.t1 MTR_4g023070 57.724 246 83 4 18 247 129 369 2.20e-89 269
MS.gene50332.t1 MTR_4g023120 56.275 247 87 4 18 248 129 370 4.85e-87 263
MS.gene50332.t1 MTR_3g103020 53.755 253 96 6 1 248 142 378 1.21e-79 244
MS.gene50332.t1 MTR_4g117720 54.618 249 96 7 1 244 137 373 1.16e-78 242
MS.gene50332.t1 MTR_4g023440 54.878 246 54 4 3 248 144 332 1.81e-77 237
MS.gene50332.t1 MTR_8g032390 54.400 250 91 9 1 244 139 371 1.23e-75 234
MS.gene50332.t1 MTR_4g116330 52.000 250 101 6 1 248 148 380 9.84e-75 232
MS.gene50332.t1 MTR_4g023150 53.386 251 88 7 1 246 132 358 1.53e-74 231
MS.gene50332.t1 MTR_6g060650 51.406 249 104 6 1 247 139 372 3.67e-74 230
MS.gene50332.t1 MTR_3g034180 54.357 241 92 7 20 248 255 489 7.07e-74 233
MS.gene50332.t1 MTR_4g023190 53.785 251 86 8 1 246 132 357 1.15e-73 228
MS.gene50332.t1 MTR_8g433030 55.983 234 88 9 17 244 114 338 8.55e-73 225
MS.gene50332.t1 MTR_4g023200 52.590 251 90 7 1 246 132 358 3.44e-72 224
MS.gene50332.t1 MTR_4g116320 58.216 213 83 5 33 242 43 252 5.15e-71 218
MS.gene50332.t1 MTR_4g117850 54.867 226 93 6 23 241 150 373 5.71e-71 222
MS.gene50332.t1 MTR_3g034130 53.478 230 91 6 20 235 141 368 2.32e-70 220
MS.gene50332.t1 MTR_4g109270 53.175 252 94 7 1 248 133 364 4.81e-70 219
MS.gene50332.t1 MTR_4g109280 56.164 219 89 6 33 248 145 359 4.97e-70 219
MS.gene50332.t1 MTR_3g034160 54.772 241 89 7 20 247 141 374 5.14e-70 219
MS.gene50332.t1 MTR_4g122850 52.549 255 99 11 1 248 125 364 5.33e-70 219
MS.gene50332.t1 MTR_4g094365 50.593 253 102 7 1 248 130 364 2.11e-69 218
MS.gene50332.t1 MTR_5g070130 49.130 230 102 5 26 244 147 372 9.24e-67 211
MS.gene50332.t1 MTR_8g433040 49.802 253 96 7 1 248 138 364 1.24e-65 208
MS.gene50332.t1 MTR_8g032380 49.802 253 96 7 1 248 138 364 1.24e-65 208
MS.gene50332.t1 MTR_8g040935 52.857 210 92 5 44 248 1 208 1.76e-65 202
MS.gene50332.t1 MTR_5g097210 49.200 250 98 9 1 243 131 358 7.57e-62 198
MS.gene50332.t1 MTR_4g117770 51.915 235 97 6 20 248 143 367 8.42e-62 198
MS.gene50332.t1 MTR_4g117810 51.915 235 97 6 20 248 143 367 8.42e-62 198
MS.gene50332.t1 MTR_5g070080 52.968 219 95 6 29 244 168 381 2.36e-61 197
MS.gene50332.t1 MTR_4g118380 48.696 230 93 6 1 218 141 357 1.80e-60 194
MS.gene50332.t1 MTR_6g463430 52.857 210 86 6 44 248 1 202 9.34e-60 187
MS.gene50332.t1 MTR_5g070088 48.661 224 101 5 29 244 164 381 4.36e-59 192
MS.gene50332.t1 MTR_4g117780 47.638 254 109 7 1 248 132 367 5.40e-59 191
MS.gene50332.t1 MTR_4g117870 45.200 250 83 9 1 248 70 267 2.65e-56 181
MS.gene50332.t1 MTR_1g055135 47.303 241 99 8 24 247 145 374 1.67e-55 182
MS.gene50332.t1 MTR_1g055195 49.115 226 87 8 29 242 263 472 3.40e-55 184
MS.gene50332.t1 MTR_4g116330 44.262 244 80 6 1 243 148 336 4.69e-52 172
MS.gene50332.t1 MTR_4g121580 45.992 237 82 9 1 230 139 336 1.63e-49 167
MS.gene50332.t1 MTR_4g117530 44.344 221 80 5 29 248 75 253 1.72e-49 163
MS.gene50332.t1 MTR_4g118400 48.325 209 81 5 41 242 106 294 1.10e-48 162
MS.gene50332.t1 MTR_4g118370 44.934 227 92 9 25 243 54 255 2.97e-48 160
MS.gene50332.t1 MTR_1g055250 43.396 212 108 8 25 229 46 252 2.26e-47 160
MS.gene50332.t1 MTR_4g117750 45.000 240 109 8 23 247 127 358 1.35e-46 161
MS.gene50332.t1 MTR_4g117750 58.716 109 42 1 135 243 373 478 3.16e-31 120
MS.gene50332.t1 MTR_7g079370 44.589 231 110 9 29 248 133 356 2.15e-45 155
MS.gene50332.t1 MTR_4g023430 87.654 81 10 0 168 248 142 222 3.69e-45 151
MS.gene50332.t1 MTR_7g079400 41.667 240 124 8 20 248 103 337 4.46e-45 154
MS.gene50332.t1 MTR_1g055175 45.960 198 95 7 57 247 8 200 4.61e-44 147
MS.gene50332.t1 MTR_8g102520 39.485 233 117 9 29 248 101 322 3.33e-39 138
MS.gene50332.t1 MTR_1g055165 49.383 162 75 5 88 247 161 317 8.00e-39 137
MS.gene50332.t1 MTR_4g114700 37.603 242 92 5 1 239 33 218 6.26e-38 133
MS.gene50332.t1 MTR_8g013010 50.000 144 58 5 1 143 178 308 3.86e-35 127
MS.gene50332.t1 MTR_4g117430 49.324 148 55 3 1 147 27 155 3.85e-34 120
MS.gene50332.t1 MTR_5g023000 60.784 102 37 1 147 248 1 99 8.06e-34 118
MS.gene50332.t1 MTR_4g118360 58.036 112 40 4 132 242 15 120 1.95e-30 110
MS.gene50332.t1 MTR_8g098830 41.860 172 81 7 54 214 31 194 4.57e-29 109
MS.gene50332.t1 MTR_4g122860 40.909 198 69 10 64 248 158 320 1.25e-28 111
MS.gene50332.t1 MTR_2g048580 34.934 229 118 10 33 248 132 342 8.79e-28 108
MS.gene50332.t1 MTR_7g079420 36.864 236 108 11 20 248 943 1144 2.70e-26 107
MS.gene50332.t1 MTR_4g023100 81.667 60 6 1 189 248 65 119 6.48e-25 95.9
MS.gene50332.t1 MTR_7g117720 64.368 87 30 1 158 243 186 272 2.69e-24 98.2
MS.gene50332.t1 MTR_7g117720 46.250 80 34 1 1 80 116 186 2.25e-12 65.5
MS.gene50332.t1 MTR_7g079450 41.549 142 76 4 107 248 3 137 5.93e-24 94.0
MS.gene50332.t1 MTR_6g034720 38.312 154 85 4 1 152 54 199 1.36e-21 89.7
MS.gene50332.t1 MTR_4g116340 54.545 88 38 2 25 111 161 247 1.92e-21 90.1
MS.gene50332.t1 MTR_4g122840 42.446 139 40 5 112 248 164 264 1.25e-19 85.5
MS.gene50332.t1 MTR_2g028130 31.016 187 116 6 17 193 153 336 8.32e-18 82.0
MS.gene50332.t1 MTR_4g133170 30.508 177 113 6 26 196 141 313 4.76e-16 77.0
MS.gene50332.t1 MTR_2g028160 31.868 182 107 6 25 193 161 338 1.76e-14 72.4
MS.gene50332.t1 MTR_1g068860 33.735 166 97 8 27 182 141 303 1.88e-14 72.4
MS.gene50332.t1 MTR_1g068875 30.168 179 109 8 29 196 141 314 2.98e-14 71.6
MS.gene50332.t1 MTR_2g025960 29.412 170 112 4 29 193 70 236 4.57e-14 70.9
MS.gene50332.t1 MTR_2g055970 27.861 201 133 8 21 213 342 538 4.69e-14 71.6
MS.gene50332.t1 MTR_0667s0010 30.978 184 104 8 1 174 136 306 6.11e-14 70.9
MS.gene50332.t1 MTR_1g068835 29.341 167 101 6 27 179 140 303 1.36e-13 69.7
MS.gene50332.t1 MTR_2g024100 32.911 158 91 7 25 170 152 306 1.59e-13 69.7
MS.gene50332.t1 MTR_4g070550 31.410 156 94 6 25 170 159 311 2.10e-13 69.3
MS.gene50332.t1 MTR_1g094210 27.320 194 120 7 29 214 196 376 2.21e-13 69.3
MS.gene50332.t1 MTR_8g101580 27.273 187 124 6 29 208 185 366 3.78e-13 68.6
MS.gene50332.t1 MTR_2g026010 31.383 188 105 9 24 193 137 318 5.42e-13 68.2
MS.gene50332.t1 MTR_2g426020 28.070 171 111 5 29 193 162 326 9.48e-13 67.4
MS.gene50332.t1 MTR_7g070440 32.121 165 98 5 27 179 148 310 1.13e-12 67.0
MS.gene50332.t1 MTR_8g013005 57.377 61 23 1 187 247 1 58 1.42e-12 61.2
MS.gene50332.t1 MTR_1g094210 27.128 188 116 7 29 208 196 370 2.53e-12 66.2
MS.gene50332.t1 MTR_2g426060 30.000 180 104 9 29 193 161 333 2.55e-12 66.2
MS.gene50332.t1 MTR_2g024110 28.191 188 116 8 29 200 141 325 5.71e-12 65.1
MS.gene50332.t1 MTR_2g022000 25.316 237 144 8 21 228 171 403 6.16e-12 65.1
MS.gene50332.t1 MTR_2g021155 30.256 195 113 11 29 208 740 926 6.27e-12 65.5
MS.gene50332.t1 MTR_4g108820 31.818 154 94 7 23 175 146 289 7.22e-12 64.3
MS.gene50332.t1 MTR_5g057690 34.118 170 93 11 23 184 170 328 8.00e-12 64.7
MS.gene50332.t1 MTR_2g031680 27.160 162 105 6 23 174 134 292 8.28e-12 64.7
MS.gene50332.t1 MTR_5g056020 27.273 253 138 11 30 240 172 420 1.02e-11 64.7
MS.gene50332.t1 MTR_1g055145 44.737 76 41 1 86 161 128 202 1.62e-11 63.2
MS.gene50332.t1 MTR_2g021150 29.775 178 111 9 27 196 157 328 2.72e-11 63.2
MS.gene50332.t1 MTR_2g425980 30.769 156 95 6 29 174 160 312 2.87e-11 63.2
MS.gene50332.t1 MTR_2g021800 24.051 237 149 7 21 229 172 405 3.13e-11 63.2
MS.gene50332.t1 MTR_4g013180 27.551 196 109 6 30 196 157 348 4.73e-11 62.4
MS.gene50332.t1 MTR_4g107780 26.939 245 135 12 17 240 164 385 5.19e-11 62.4
MS.gene50332.t1 MTR_4g107820 26.939 245 135 12 17 240 164 385 5.64e-11 62.0
MS.gene50332.t1 MTR_4g107780 26.939 245 135 12 17 240 164 385 5.64e-11 62.0
MS.gene50332.t1 MTR_4g107820 26.939 245 135 12 17 240 164 385 5.64e-11 62.4
MS.gene50332.t1 MTR_5g057710 34.161 161 86 10 23 176 185 332 6.49e-11 62.0
MS.gene50332.t1 MTR_3g036290 32.911 158 84 7 28 181 161 300 8.96e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50332.t1 AT3G06240 31.282 195 122 6 29 220 215 400 2.73e-16 77.8

Find 60 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGAATAGCCCCATTAAAT+AGG 0.222547 4.4:-78274208 None:intergenic
CGCCTTCATATACTTGGTTT+TGG 0.241496 4.4:+78274014 MS.gene50332:CDS
TCTCATTTAGAGATAATAAA+TGG 0.250501 4.4:+78274126 MS.gene50332:CDS
ATGACAATGTGCTGCTAATT+TGG 0.251004 4.4:-78273967 None:intergenic
CATTGTTGCCTTTGATTTAA+CGG 0.288851 4.4:+78274265 MS.gene50332:CDS
CCAGTCAAGAGATGATTATT+TGG 0.297387 4.4:+78274043 MS.gene50332:CDS
AATTCTAGTTTGTGGGTATA+TGG 0.300528 4.4:+78274338 MS.gene50332:CDS
TATTTAATGGGGCTATTCAT+TGG 0.316240 4.4:+78274210 MS.gene50332:CDS
ATGATGAAGGACAGCTATTA+AGG 0.321316 4.4:+78274567 MS.gene50332:CDS
CTAATGATACAGTTGAAATA+TGG 0.329864 4.4:+78274393 MS.gene50332:CDS
AGAAAAGGGTCCCTATTTAA+TGG 0.349556 4.4:+78274197 MS.gene50332:CDS
GTATTTAGTTGTCATGTATA+CGG 0.356000 4.4:+78274628 MS.gene50332:CDS
GAAAAGGGTCCCTATTTAAT+GGG 0.364953 4.4:+78274198 MS.gene50332:CDS
ATGGCTTTGAGTCATGATTT+AGG 0.366864 4.4:+78274233 MS.gene50332:CDS
TGTAGTTAATTCTAGTTTGT+GGG 0.415557 4.4:+78274331 MS.gene50332:CDS
ATCTTCTACCATTGGATCAT+AGG 0.418878 4.4:-78274078 None:intergenic
GCAGCACATTGTCATCGTAT+TGG 0.424130 4.4:+78273975 MS.gene50332:CDS
GTCAATCATAGTCGCAGAAA+AGG 0.424575 4.4:+78274182 MS.gene50332:CDS
ATGAATAGCCCCATTAAATA+GGG 0.428993 4.4:-78274207 None:intergenic
TCTTATCGCCTTCATATACT+TGG 0.436134 4.4:+78274008 MS.gene50332:CDS
GATATTATCGGAGGAAATCT+TGG 0.436612 4.4:+78274524 MS.gene50332:CDS
ATGTAGTTAATTCTAGTTTG+TGG 0.445103 4.4:+78274330 MS.gene50332:CDS
ATTTCTCAGTCGATGGGCTA+TGG 0.462969 4.4:+78274364 MS.gene50332:CDS
GAGTCTCTGCTTTCGCTCCC+TGG 0.464262 4.4:+78274650 MS.gene50332:CDS
CTTTGAGTCATGATTTAGGT+TGG 0.471414 4.4:+78274237 MS.gene50332:CDS
GAAACAGCATCTTCTACCAT+TGG 0.482329 4.4:-78274086 None:intergenic
AGGAAATCTTGGTACTAGAT+TGG 0.488834 4.4:+78274535 MS.gene50332:CDS
TAATAAATGGAAAGAAATTG+AGG 0.493163 4.4:+78274139 MS.gene50332:CDS
AATACAAAGTGCATTCGTCT+TGG 0.494278 4.4:+78274426 MS.gene50332:CDS
TAATGGGGCTATTCATTGGA+TGG 0.494477 4.4:+78274214 MS.gene50332:CDS
GTCAAGAGATGATTATTTGG+TGG 0.498135 4.4:+78274046 MS.gene50332:CDS
ATTGGGTGAAAGTATCGATC+AGG 0.504656 4.4:-78274482 None:intergenic
AACTAAATACATGACAAGTT+CGG 0.506028 4.4:-78274615 None:intergenic
GGTGGCATCGAGCAAGTGTA+AGG 0.507282 4.4:+78274671 MS.gene50332:CDS
TACACTTGCTCGATGCCACC+AGG 0.518184 4.4:-78274668 None:intergenic
ATGGAGAATTTCTCAGTCGA+TGG 0.520871 4.4:+78274357 MS.gene50332:CDS
AACCAAAACCAAGTATATGA+AGG 0.524098 4.4:-78274016 None:intergenic
CCAAATAATCATCTCTTGAC+TGG 0.527790 4.4:-78274043 None:intergenic
TCAATCATAGTCGCAGAAAA+GGG 0.531989 4.4:+78274183 MS.gene50332:CDS
TTCATTGTCCTATGATCCAA+TGG 0.536743 4.4:+78274070 MS.gene50332:CDS
GCTGCTAATTTGGAGTCAAA+AGG 0.537694 4.4:-78273957 None:intergenic
GTATATGAAGGCGATAAGAC+TGG 0.541415 4.4:-78274004 None:intergenic
TTGGTGAAGTATAATGATGA+AGG 0.541886 4.4:+78274554 MS.gene50332:CDS
TAATGATACAGTTGAAATAT+GGG 0.542942 4.4:+78274394 MS.gene50332:CDS
AATAAATGGAAAGAAATTGA+GGG 0.552425 4.4:+78274140 MS.gene50332:CDS
ACTACATAGTCAAACTCACT+TGG 0.565813 4.4:-78274314 None:intergenic
TAGTCAAACTCACTTGGAAG+AGG 0.576522 4.4:-78274308 None:intergenic
GATGCTGTTTCAACACATGT+GGG 0.589760 4.4:+78274098 MS.gene50332:CDS
TGGAGAATTTCTCAGTCGAT+GGG 0.597386 4.4:+78274358 MS.gene50332:CDS
AGATGCTGTTTCAACACATG+TGG 0.597758 4.4:+78274097 MS.gene50332:CDS
TTCGGATGGACTAGTGCAGA+AGG 0.600027 4.4:-78274597 None:intergenic
AAATACATGACAAGTTCGGA+TGG 0.609589 4.4:-78274611 None:intergenic
CGATCAGGAATTGCATGAAG+AGG 0.610810 4.4:-78274467 None:intergenic
TTGGAGTCAAAAGGAGACAC+GGG 0.631270 4.4:-78273948 None:intergenic
ATGCTGTTTCAACACATGTG+GGG 0.642270 4.4:+78274099 MS.gene50332:CDS
TTTGGAGTCAAAAGGAGACA+CGG 0.650067 4.4:-78273949 None:intergenic
TCTCTGCTTTCGCTCCCTGG+TGG 0.656790 4.4:+78274653 MS.gene50332:CDS
AAAAGGGTCCCTATTTAATG+GGG 0.672236 4.4:+78274199 MS.gene50332:CDS
GACTATGATTGACATCGTAA+CGG 0.674942 4.4:-78274172 None:intergenic
ACACTTGCTCGATGCCACCA+GGG 0.698396 4.4:-78274667 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATGGAAAGAAATTGA+GGG + chr4.4:78274140-78274159 MS.gene50332:CDS 20.0%
!! ACAAAAAATGGTGATATTAT+CGG + chr4.4:78274512-78274531 MS.gene50332:CDS 20.0%
!! TAATAAATGGAAAGAAATTG+AGG + chr4.4:78274139-78274158 MS.gene50332:CDS 20.0%
!! TAATGATACAGTTGAAATAT+GGG + chr4.4:78274394-78274413 MS.gene50332:CDS 20.0%
!! TCTCATTTAGAGATAATAAA+TGG + chr4.4:78274126-78274145 MS.gene50332:CDS 20.0%
! AACTAAATACATGACAAGTT+CGG - chr4.4:78274618-78274637 None:intergenic 25.0%
! CTAATGATACAGTTGAAATA+TGG + chr4.4:78274393-78274412 MS.gene50332:CDS 25.0%
! GTATTTAGTTGTCATGTATA+CGG + chr4.4:78274628-78274647 MS.gene50332:CDS 25.0%
!!! ATGTAGTTAATTCTAGTTTG+TGG + chr4.4:78274330-78274349 MS.gene50332:CDS 25.0%
!!! CATTTTTTGTAGAGTACATT+GGG - chr4.4:78274502-78274521 None:intergenic 25.0%
!!! TGTAGTTAATTCTAGTTTGT+GGG + chr4.4:78274331-78274350 MS.gene50332:CDS 25.0%
AAAAATGGTGATATTATCGG+AGG + chr4.4:78274515-78274534 MS.gene50332:CDS 30.0%
AACCAAAACCAAGTATATGA+AGG - chr4.4:78274019-78274038 None:intergenic 30.0%
AATGAATAGCCCCATTAAAT+AGG - chr4.4:78274211-78274230 None:intergenic 30.0%
ATGAATAGCCCCATTAAATA+GGG - chr4.4:78274210-78274229 None:intergenic 30.0%
CCAATGTACTCTACAAAAAA+TGG + chr4.4:78274500-78274519 MS.gene50332:CDS 30.0%
TATTTAATGGGGCTATTCAT+TGG + chr4.4:78274210-78274229 MS.gene50332:CDS 30.0%
TTGGTGAAGTATAATGATGA+AGG + chr4.4:78274554-78274573 MS.gene50332:CDS 30.0%
! CATTGTTGCCTTTGATTTAA+CGG + chr4.4:78274265-78274284 MS.gene50332:CDS 30.0%
! TTTTCGTTCCGTTAAATCAA+AGG - chr4.4:78274276-78274295 None:intergenic 30.0%
!! AATTCTAGTTTGTGGGTATA+TGG + chr4.4:78274338-78274357 MS.gene50332:CDS 30.0%
!!! CCATTTTTTGTAGAGTACAT+TGG - chr4.4:78274503-78274522 None:intergenic 30.0%
AAAAGGGTCCCTATTTAATG+GGG + chr4.4:78274199-78274218 MS.gene50332:CDS 35.0%
AAATACATGACAAGTTCGGA+TGG - chr4.4:78274614-78274633 None:intergenic 35.0%
AATACAAAGTGCATTCGTCT+TGG + chr4.4:78274426-78274445 MS.gene50332:CDS 35.0%
ACTACATAGTCAAACTCACT+TGG - chr4.4:78274317-78274336 None:intergenic 35.0%
AGAAAAGGGTCCCTATTTAA+TGG + chr4.4:78274197-78274216 MS.gene50332:CDS 35.0%
AGGAAATCTTGGTACTAGAT+TGG + chr4.4:78274535-78274554 MS.gene50332:CDS 35.0%
ATCTTCTACCATTGGATCAT+AGG - chr4.4:78274081-78274100 None:intergenic 35.0%
ATGATGAAGGACAGCTATTA+AGG + chr4.4:78274567-78274586 MS.gene50332:CDS 35.0%
CCAAATAATCATCTCTTGAC+TGG - chr4.4:78274046-78274065 None:intergenic 35.0%
CCAGTCAAGAGATGATTATT+TGG + chr4.4:78274043-78274062 MS.gene50332:CDS 35.0%
CTTTGAGTCATGATTTAGGT+TGG + chr4.4:78274237-78274256 MS.gene50332:CDS 35.0%
GAAAAGGGTCCCTATTTAAT+GGG + chr4.4:78274198-78274217 MS.gene50332:CDS 35.0%
GACTATGATTGACATCGTAA+CGG - chr4.4:78274175-78274194 None:intergenic 35.0%
GATATTATCGGAGGAAATCT+TGG + chr4.4:78274524-78274543 MS.gene50332:CDS 35.0%
GTCAAGAGATGATTATTTGG+TGG + chr4.4:78274046-78274065 MS.gene50332:CDS 35.0%
TCAATCATAGTCGCAGAAAA+GGG + chr4.4:78274183-78274202 MS.gene50332:CDS 35.0%
TCTTATCGCCTTCATATACT+TGG + chr4.4:78274008-78274027 MS.gene50332:CDS 35.0%
TTCATTGTCCTATGATCCAA+TGG + chr4.4:78274070-78274089 MS.gene50332:CDS 35.0%
! ATGACAATGTGCTGCTAATT+TGG - chr4.4:78273970-78273989 None:intergenic 35.0%
!! ATGGCTTTGAGTCATGATTT+AGG + chr4.4:78274233-78274252 MS.gene50332:CDS 35.0%
ATGGAGAATTTCTCAGTCGA+TGG + chr4.4:78274357-78274376 MS.gene50332:CDS 40.0%
GAAACAGCATCTTCTACCAT+TGG - chr4.4:78274089-78274108 None:intergenic 40.0%
GTATATGAAGGCGATAAGAC+TGG - chr4.4:78274007-78274026 None:intergenic 40.0%
GTCAATCATAGTCGCAGAAA+AGG + chr4.4:78274182-78274201 MS.gene50332:CDS 40.0%
TAATGGGGCTATTCATTGGA+TGG + chr4.4:78274214-78274233 MS.gene50332:CDS 40.0%
TAGTCAAACTCACTTGGAAG+AGG - chr4.4:78274311-78274330 None:intergenic 40.0%
TGGAGAATTTCTCAGTCGAT+GGG + chr4.4:78274358-78274377 MS.gene50332:CDS 40.0%
! CGCCTTCATATACTTGGTTT+TGG + chr4.4:78274014-78274033 MS.gene50332:CDS 40.0%
! GCTGCTAATTTGGAGTCAAA+AGG - chr4.4:78273960-78273979 None:intergenic 40.0%
! TTTGGAGTCAAAAGGAGACA+CGG - chr4.4:78273952-78273971 None:intergenic 40.0%
!! AGATGCTGTTTCAACACATG+TGG + chr4.4:78274097-78274116 MS.gene50332:CDS 40.0%
!! ATGCTGTTTCAACACATGTG+GGG + chr4.4:78274099-78274118 MS.gene50332:CDS 40.0%
!! ATTGGGTGAAAGTATCGATC+AGG - chr4.4:78274485-78274504 None:intergenic 40.0%
!! GATGCTGTTTCAACACATGT+GGG + chr4.4:78274098-78274117 MS.gene50332:CDS 40.0%
CGATCAGGAATTGCATGAAG+AGG - chr4.4:78274470-78274489 None:intergenic 45.0%
GCAGCACATTGTCATCGTAT+TGG + chr4.4:78273975-78273994 MS.gene50332:CDS 45.0%
TTGGAGTCAAAAGGAGACAC+GGG - chr4.4:78273951-78273970 None:intergenic 45.0%
!! ATTTCTCAGTCGATGGGCTA+TGG + chr4.4:78274364-78274383 MS.gene50332:CDS 45.0%
!! TTCGGATGGACTAGTGCAGA+AGG - chr4.4:78274600-78274619 None:intergenic 50.0%
ACACTTGCTCGATGCCACCA+GGG - chr4.4:78274670-78274689 None:intergenic 55.0%
TACACTTGCTCGATGCCACC+AGG - chr4.4:78274671-78274690 None:intergenic 55.0%
GAGTCTCTGCTTTCGCTCCC+TGG + chr4.4:78274650-78274669 MS.gene50332:CDS 60.0%
TCTCTGCTTTCGCTCCCTGG+TGG + chr4.4:78274653-78274672 MS.gene50332:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 78273945 78274691 78273945 ID=MS.gene50332
chr4.4 mRNA 78273945 78274691 78273945 ID=MS.gene50332.t1;Parent=MS.gene50332
chr4.4 exon 78273945 78274691 78273945 ID=MS.gene50332.t1.exon1;Parent=MS.gene50332.t1
chr4.4 CDS 78273945 78274691 78273945 ID=cds.MS.gene50332.t1;Parent=MS.gene50332.t1
Gene Sequence

>MS.gene50332

ATGCCCGTGTCTCCTTTTGACTCCAAATTAGCAGCACATTGTCATCGTATTGGTTATGACCAGTCTTATCGCCTTCATATACTTGGTTTTGGTTATGACCAGTCAAGAGATGATTATTTGGTGGTTTCATTGTCCTATGATCCAATGGTAGAAGATGCTGTTTCAACACATGTGGGGTTTTTCTCATTTAGAGATAATAAATGGAAAGAAATTGAGGGTACTCACTTCCGTTACGATGTCAATCATAGTCGCAGAAAAGGGTCCCTATTTAATGGGGCTATTCATTGGATGGCTTTGAGTCATGATTTAGGTTGGAGTGTCATTGTTGCCTTTGATTTAACGGAACGAAAATTGTTTGAGATGCCTCTTCCAAGTGAGTTTGACTATGTAGTTAATTCTAGTTTGTGGGTATATGGAGAATTTCTCAGTCGATGGGCTATGGATTATGCTAATGATACAGTTGAAATATGGGTGATGAAAGAATACAAAGTGCATTCGTCTTGGACTAAAACTCTTGTTCTTCCTCTTCATGCAATTCCTGATCGATACTTTCACCCAATGTACTCTACAAAAAATGGTGATATTATCGGAGGAAATCTTGGTACTAGATTGGTGAAGTATAATGATGAAGGACAGCTATTAAGGCATCGCTCCTTCTGCACTAGTCCATCCGAACTTGTCATGTATTTAGTTGTCATGTATACGGAGTCTCTGCTTTCGCTCCCTGGTGGCATCGAGCAAGTGTAA
Protein sequence

>MS.gene50332.t1

MPVSPFDSKLAAHCHRIGYDQSYRLHILGFGYDQSRDDYLVVSLSYDPMVEDAVSTHVGFFSFRDNKWKEIEGTHFRYDVNHSRRKGSLFNGAIHWMALSHDLGWSVIVAFDLTERKLFEMPLPSEFDYVVNSSLWVYGEFLSRWAMDYANDTVEIWVMKEYKVHSSWTKTLVLPLHAIPDRYFHPMYSTKNGDIIGGNLGTRLVKYNDEGQLLRHRSFCTSPSELVMYLVVMYTESLLSLPGGIEQV