AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40982


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40982.t1 MTR_1g055175 77.301 163 35 1 1 163 41 201 5.85e-88 256
MS.gene40982.t1 MTR_1g055165 77.301 163 35 1 1 163 158 318 5.48e-87 257
MS.gene40982.t1 MTR_1g055135 73.939 165 40 2 1 163 212 375 6.69e-78 236
MS.gene40982.t1 MTR_1g055195 64.417 163 55 3 1 163 319 478 2.12e-61 197
MS.gene40982.t1 MTR_5g097210 66.456 158 49 3 2 159 206 359 3.24e-61 193
MS.gene40982.t1 MTR_1g055250 66.423 137 42 3 1 137 111 243 2.73e-55 177
MS.gene40982.t1 MTR_4g117720 58.580 169 60 7 2 163 213 378 2.65e-52 171
MS.gene40982.t1 MTR_6g060650 56.442 163 66 4 1 162 214 372 6.53e-52 169
MS.gene40982.t1 MTR_4g109280 57.669 163 61 6 4 163 202 359 8.94e-50 164
MS.gene40982.t1 MTR_4g118370 55.000 160 50 5 1 160 120 257 9.89e-47 153
MS.gene40982.t1 MTR_4g117750 56.707 164 63 4 1 159 195 355 1.48e-46 158
MS.gene40982.t1 MTR_4g117750 48.182 110 54 3 54 161 373 481 8.24e-23 94.0
MS.gene40982.t1 MTR_3g103020 56.173 162 64 5 5 163 221 378 8.19e-46 154
MS.gene40982.t1 MTR_4g116330 52.381 168 71 6 1 163 217 380 8.27e-46 154
MS.gene40982.t1 MTR_4g094365 52.147 163 74 3 2 162 203 363 1.11e-44 150
MS.gene40982.t1 MTR_4g122850 50.932 161 74 4 5 163 207 364 3.01e-43 147
MS.gene40982.t1 MTR_4g116320 52.439 164 67 5 2 160 98 255 5.27e-43 143
MS.gene40982.t1 MTR_3g034180 51.852 162 72 5 2 162 332 488 9.01e-43 148
MS.gene40982.t1 MTR_6g463430 52.201 159 69 4 6 163 50 202 2.35e-42 140
MS.gene40982.t1 MTR_4g118380 56.338 142 51 4 1 133 216 355 1.48e-41 142
MS.gene40982.t1 MTR_5g070080 52.976 168 66 7 2 163 226 386 1.55e-41 143
MS.gene40982.t1 MTR_5g070088 51.572 159 73 4 1 157 223 379 6.55e-41 141
MS.gene40982.t1 MTR_4g117850 52.201 159 67 5 4 156 218 373 1.09e-40 140
MS.gene40982.t1 MTR_8g040935 52.096 167 67 7 5 163 47 208 1.21e-40 135
MS.gene40982.t1 MTR_4g109270 51.553 161 71 5 6 163 208 364 2.23e-40 139
MS.gene40982.t1 MTR_8g433030 53.503 157 65 6 4 157 185 336 2.22e-39 136
MS.gene40982.t1 MTR_8g032390 53.503 157 65 6 4 157 218 369 5.89e-39 135
MS.gene40982.t1 MTR_4g023130 50.311 161 70 6 5 162 159 312 2.50e-38 133
MS.gene40982.t1 MTR_5g070130 52.258 155 68 5 6 157 219 370 2.72e-38 134
MS.gene40982.t1 MTR_4g023080 50.311 161 70 6 5 162 200 353 4.20e-38 133
MS.gene40982.t1 MTR_4g023070 48.837 172 70 6 5 162 202 369 1.97e-37 132
MS.gene40982.t1 MTR_3g034160 51.235 162 73 4 2 162 218 374 3.21e-37 131
MS.gene40982.t1 MTR_4g021785 48.447 161 75 5 5 163 201 355 6.59e-37 130
MS.gene40982.t1 MTR_3g034130 48.993 149 71 4 2 149 218 362 4.37e-36 128
MS.gene40982.t1 MTR_4g023380 43.784 185 73 7 5 163 200 379 5.62e-36 128
MS.gene40982.t1 MTR_8g433040 51.408 142 63 3 23 163 228 364 9.32e-36 127
MS.gene40982.t1 MTR_8g032380 51.408 142 63 3 23 163 228 364 9.32e-36 127
MS.gene40982.t1 MTR_4g118400 49.686 159 61 6 1 157 153 294 1.12e-35 125
MS.gene40982.t1 MTR_4g118360 50.781 128 60 3 37 163 1 126 4.40e-35 119
MS.gene40982.t1 MTR_4g023090 44.571 175 76 7 5 162 215 385 5.55e-35 125
MS.gene40982.t1 MTR_7g079400 45.679 162 82 3 6 163 178 337 1.39e-34 124
MS.gene40982.t1 MTR_4g117780 45.732 164 85 4 2 162 204 366 3.96e-34 123
MS.gene40982.t1 MTR_4g117770 46.341 164 84 4 2 162 204 366 7.51e-34 122
MS.gene40982.t1 MTR_4g117810 46.341 164 84 4 2 162 204 366 7.51e-34 122
MS.gene40982.t1 MTR_4g023120 45.455 176 72 6 5 163 202 370 1.75e-33 121
MS.gene40982.t1 MTR_4g023150 47.239 163 75 6 5 161 201 358 1.99e-33 121
MS.gene40982.t1 MTR_7g079420 45.000 160 83 4 6 163 988 1144 2.35e-33 124
MS.gene40982.t1 MTR_4g023190 47.853 163 74 6 5 161 200 357 5.25e-33 120
MS.gene40982.t1 MTR_4g023200 46.012 163 77 6 5 161 201 358 2.27e-32 118
MS.gene40982.t1 MTR_4g117870 47.692 130 65 2 34 163 141 267 1.32e-30 112
MS.gene40982.t1 MTR_8g102520 40.741 162 94 2 2 163 163 322 2.91e-30 112
MS.gene40982.t1 MTR_4g117530 41.875 160 57 4 5 163 129 253 7.22e-30 109
MS.gene40982.t1 MTR_7g079370 43.871 155 80 5 6 158 202 351 1.75e-28 108
MS.gene40982.t1 MTR_5g023000 56.250 96 34 5 72 163 8 99 1.48e-24 91.7
MS.gene40982.t1 MTR_7g079450 40.426 141 78 3 23 163 3 137 2.69e-24 92.0
MS.gene40982.t1 MTR_4g116330 39.286 168 54 6 1 163 217 341 2.94e-23 94.0
MS.gene40982.t1 MTR_2g048580 35.976 164 91 6 2 163 191 342 5.81e-23 93.2
MS.gene40982.t1 MTR_4g122860 38.095 168 69 6 1 162 181 319 9.88e-23 92.4
MS.gene40982.t1 MTR_4g023440 38.125 160 55 5 5 162 214 331 1.25e-22 92.0
MS.gene40982.t1 MTR_1g055145 62.338 77 26 1 3 79 129 202 4.33e-22 89.7
MS.gene40982.t1 MTR_8g098830 37.692 130 78 3 2 131 68 194 6.23e-17 75.1
MS.gene40982.t1 MTR_7g117720 41.905 105 49 4 62 158 172 272 6.95e-15 70.5
MS.gene40982.t1 MTR_4g121580 40.157 127 44 6 5 129 222 318 9.57e-15 70.9
MS.gene40982.t1 MTR_8g013005 55.000 60 26 1 104 163 1 59 1.45e-13 62.4
MS.gene40982.t1 MTR_4g114700 43.182 88 47 2 70 154 131 218 1.93e-13 65.9
MS.gene40982.t1 MTR_8g013010 58.065 62 20 1 2 63 254 309 2.84e-13 66.2
MS.gene40982.t1 MTR_4g023100 43.056 72 37 2 92 162 50 118 9.71e-13 61.6
MS.gene40982.t1 MTR_4g117430 55.932 59 24 1 5 63 96 152 1.98e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTTGCCTTTCATTTAA+TGG 0.113478 1.3:+40623648 MS.gene40982:CDS
GTTTCAAAGATTGTAATTTA+TGG 0.151549 1.3:+40623722 MS.gene40982:CDS
TTTCTCAGTCTATGGGTTTC+GGG 0.238313 1.3:+40623757 MS.gene40982:CDS
TTAGAGGATTTCTCAGTCTA+TGG 0.277856 1.3:+40623749 MS.gene40982:CDS
ACAAGGGTAAAGTTGATATA+TGG 0.287530 1.3:+40623782 MS.gene40982:CDS
ATTTCTCAGTCTATGGGTTT+CGG 0.288923 1.3:+40623756 MS.gene40982:CDS
TTTCAAAGATTGTAATTTAT+GGG 0.304574 1.3:+40623723 MS.gene40982:CDS
TATTCAAAATCATCATCATT+TGG 0.309421 1.3:-40623697 None:intergenic
ATTCAAAATCATCATCATTT+GGG 0.373726 1.3:-40623696 None:intergenic
TGTAATTTATGGGTATTTAG+AGG 0.380678 1.3:+40623733 MS.gene40982:CDS
TCTTCAATGGGGCTATTCAC+TGG 0.419380 1.3:+40623593 MS.gene40982:CDS
CAAGGGTAAAGTTGATATAT+GGG 0.420388 1.3:+40623783 MS.gene40982:CDS
AATACAAAGTGCAGTCATCT+TGG 0.424763 1.3:+40623815 MS.gene40982:CDS
TGGTAGAGTCAGTCTTCAAT+GGG 0.445013 1.3:+40623581 MS.gene40982:CDS
ATGGTAGAGTCAGTCTTCAA+TGG 0.450181 1.3:+40623580 MS.gene40982:CDS
CAATGGGGCTATTCACTGGT+TGG 0.483990 1.3:+40623597 MS.gene40982:CDS
TTGCCTTTCATTTAATGGAA+AGG 0.488920 1.3:+40623653 MS.gene40982:CDS
TAGAGGATTTCTCAGTCTAT+GGG 0.489737 1.3:+40623750 MS.gene40982:CDS
TTCTCAGTCTATGGGTTTCG+GGG 0.502147 1.3:+40623758 MS.gene40982:CDS
TAAGCTTCCTCATTGTCACT+AGG 0.504127 1.3:-40624048 None:intergenic
TATCCTTTCCATTAAATGAA+AGG 0.507802 1.3:-40623656 None:intergenic
GTTGGAACAGATTACCGTAC+TGG 0.514745 1.3:+40623916 MS.gene40982:CDS
TACAAGGGCAACGTTATGAA+TGG 0.537235 1.3:-40623869 None:intergenic
AAATCATCATCATTTGGGAG+AGG 0.551980 1.3:-40623691 None:intergenic
AAGCTTCCTCATTGTCACTA+GGG 0.552135 1.3:-40624047 None:intergenic
TTCACGAAATGAATTCCGAT+TGG 0.558524 1.3:+40623996 MS.gene40982:CDS
GAACAGATTACCGTACTGGA+TGG 0.571251 1.3:+40623920 MS.gene40982:CDS
ACTCTGTATACATAGCCAAT+CGG 0.573189 1.3:-40624011 None:intergenic
CATATCTCACCCATCCAGTA+CGG 0.584321 1.3:-40623930 None:intergenic
AACAGATTACCGTACTGGAT+GGG 0.623237 1.3:+40623921 MS.gene40982:CDS
TTCACTCCCTAGTGACAATG+AGG 0.626833 1.3:+40624041 MS.gene40982:CDS
TCTCAGTCTATGGGTTTCGG+GGG 0.637073 1.3:+40623759 MS.gene40982:CDS
GCTATTCGTCACGATAGACG+TGG 0.699166 1.3:+40623619 MS.gene40982:CDS
CTATTCGTCACGATAGACGT+GGG 0.735650 1.3:+40623620 MS.gene40982:CDS
TGGGTGAGATATGATGACAA+AGG 0.747254 1.3:+40623940 MS.gene40982:CDS
GGTAGAGTCAGTCTTCAATG+GGG 0.775884 1.3:+40623582 MS.gene40982:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCAAAGATTGTAATTTAT+GGG + chr1.3:40623723-40623742 MS.gene40982:CDS 15.0%
!! ATTCAAAATCATCATCATTT+GGG - chr1.3:40623699-40623718 None:intergenic 20.0%
!! GTTTCAAAGATTGTAATTTA+TGG + chr1.3:40623722-40623741 MS.gene40982:CDS 20.0%
!! TATTCAAAATCATCATCATT+TGG - chr1.3:40623700-40623719 None:intergenic 20.0%
! TATCCTTTCCATTAAATGAA+AGG - chr1.3:40623659-40623678 None:intergenic 25.0%
! TATTGTTGCCTTTCATTTAA+TGG + chr1.3:40623648-40623667 MS.gene40982:CDS 25.0%
! TGTAATTTATGGGTATTTAG+AGG + chr1.3:40623733-40623752 MS.gene40982:CDS 25.0%
ACAAAAAGTGGTGATATTGT+TGG + chr1.3:40623898-40623917 MS.gene40982:CDS 30.0%
TTGCCTTTCATTTAATGGAA+AGG + chr1.3:40623653-40623672 MS.gene40982:CDS 30.0%
! ACTTTTTGTGCAACATACAA+GGG - chr1.3:40623887-40623906 None:intergenic 30.0%
!! ACAAGGGTAAAGTTGATATA+TGG + chr1.3:40623782-40623801 MS.gene40982:CDS 30.0%
!! CAAGGGTAAAGTTGATATAT+GGG + chr1.3:40623783-40623802 MS.gene40982:CDS 30.0%
AAATCATCATCATTTGGGAG+AGG - chr1.3:40623694-40623713 None:intergenic 35.0%
AATACAAAGTGCAGTCATCT+TGG + chr1.3:40623815-40623834 MS.gene40982:CDS 35.0%
ACTCTGTATACATAGCCAAT+CGG - chr1.3:40624014-40624033 None:intergenic 35.0%
ATTTCTCAGTCTATGGGTTT+CGG + chr1.3:40623756-40623775 MS.gene40982:CDS 35.0%
CTTGTATGTTGCACAAAAAG+TGG + chr1.3:40623886-40623905 MS.gene40982:CDS 35.0%
TAGAGGATTTCTCAGTCTAT+GGG + chr1.3:40623750-40623769 MS.gene40982:CDS 35.0%
TTAGAGGATTTCTCAGTCTA+TGG + chr1.3:40623749-40623768 MS.gene40982:CDS 35.0%
TTCACGAAATGAATTCCGAT+TGG + chr1.3:40623996-40624015 MS.gene40982:CDS 35.0%
!! CACTTTTTGTGCAACATACA+AGG - chr1.3:40623888-40623907 None:intergenic 35.0%
AACAGATTACCGTACTGGAT+GGG + chr1.3:40623921-40623940 MS.gene40982:CDS 40.0%
AAGCTTCCTCATTGTCACTA+GGG - chr1.3:40624050-40624069 None:intergenic 40.0%
ATGGTAGAGTCAGTCTTCAA+TGG + chr1.3:40623580-40623599 MS.gene40982:CDS 40.0%
TAAGCTTCCTCATTGTCACT+AGG - chr1.3:40624051-40624070 None:intergenic 40.0%
TACAAGGGCAACGTTATGAA+TGG - chr1.3:40623872-40623891 None:intergenic 40.0%
TGGGTGAGATATGATGACAA+AGG + chr1.3:40623940-40623959 MS.gene40982:CDS 40.0%
TGGTAGAGTCAGTCTTCAAT+GGG + chr1.3:40623581-40623600 MS.gene40982:CDS 40.0%
TTTCTCAGTCTATGGGTTTC+GGG + chr1.3:40623757-40623776 MS.gene40982:CDS 40.0%
CATATCTCACCCATCCAGTA+CGG - chr1.3:40623933-40623952 None:intergenic 45.0%
CTATTCGTCACGATAGACGT+GGG + chr1.3:40623620-40623639 MS.gene40982:CDS 45.0%
GAACAGATTACCGTACTGGA+TGG + chr1.3:40623920-40623939 MS.gene40982:CDS 45.0%
GGTAGAGTCAGTCTTCAATG+GGG + chr1.3:40623582-40623601 MS.gene40982:CDS 45.0%
GTTGGAACAGATTACCGTAC+TGG + chr1.3:40623916-40623935 MS.gene40982:CDS 45.0%
TCTTCAATGGGGCTATTCAC+TGG + chr1.3:40623593-40623612 MS.gene40982:CDS 45.0%
TTCACTCCCTAGTGACAATG+AGG + chr1.3:40624041-40624060 MS.gene40982:CDS 45.0%
TTCTCAGTCTATGGGTTTCG+GGG + chr1.3:40623758-40623777 MS.gene40982:CDS 45.0%
CAATGGGGCTATTCACTGGT+TGG + chr1.3:40623597-40623616 MS.gene40982:CDS 50.0%
GCTATTCGTCACGATAGACG+TGG + chr1.3:40623619-40623638 MS.gene40982:CDS 50.0%
TCTCAGTCTATGGGTTTCGG+GGG + chr1.3:40623759-40623778 MS.gene40982:CDS 50.0%
CTATGGGTTTCGGGGGACAA+GGG + chr1.3:40623766-40623785 MS.gene40982:CDS 55.0%
TCTATGGGTTTCGGGGGACA+AGG + chr1.3:40623765-40623784 MS.gene40982:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 40623580 40624071 40623580 ID=MS.gene40982
chr1.3 mRNA 40623580 40624071 40623580 ID=MS.gene40982.t1;Parent=MS.gene40982
chr1.3 exon 40623580 40624071 40623580 ID=MS.gene40982.t1.exon1;Parent=MS.gene40982.t1
chr1.3 CDS 40623580 40624071 40623580 ID=cds.MS.gene40982.t1;Parent=MS.gene40982.t1
Gene Sequence

>MS.gene40982

ATGGTAGAGTCAGTCTTCAATGGGGCTATTCACTGGTTGGCTATTCGTCACGATAGACGTGGGTATGTTATTGTTGCCTTTCATTTAATGGAAAGGATACTTCTAGAAATTCCTCTCCCAAATGATGATGATTTTGAATATAGTTTCAAAGATTGTAATTTATGGGTATTTAGAGGATTTCTCAGTCTATGGGTTTCGGGGGACAAGGGTAAAGTTGATATATGGGTGATGAAAGAATACAAAGTGCAGTCATCTTGGACTAAAACTCTTGTTCTTACTACTTATGATACCATTCATAACGTTGCCCTTGTATGTTGCACAAAAAGTGGTGATATTGTTGGAACAGATTACCGTACTGGATGGGTGAGATATGATGACAAAGGAGAGTTTCTAGAGCATATCTACTATTGCATAGATTCACGAAATGAATTCCGATTGGCTATGTATACAGAGTCTTTGCTTTCACTCCCTAGTGACAATGAGGAAGCTTAA

Protein sequence

>MS.gene40982.t1

MVESVFNGAIHWLAIRHDRRGYVIVAFHLMERILLEIPLPNDDDFEYSFKDCNLWVFRGFLSLWVSGDKGKVDIWVMKEYKVQSSWTKTLVLTTYDTIHNVALVCCTKSGDIVGTDYRTGWVRYDDKGEFLEHIYYCIDSRNEFRLAMYTESLLSLPSDNEEA