AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene041475


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041475.t1 MTR_4g117780 57.848 223 82 6 3 217 149 367 1.59e-70 219
MS.gene041475.t1 MTR_4g117770 57.848 223 82 6 3 217 149 367 3.52e-68 213
MS.gene041475.t1 MTR_4g117810 57.848 223 82 6 3 217 149 367 3.52e-68 213
MS.gene041475.t1 MTR_6g060650 55.556 225 80 6 3 216 157 372 3.96e-67 211
MS.gene041475.t1 MTR_4g117850 55.752 226 75 7 3 210 155 373 2.23e-65 206
MS.gene041475.t1 MTR_4g117720 55.365 233 77 9 3 217 155 378 3.40e-65 206
MS.gene041475.t1 MTR_4g023130 54.795 219 84 6 3 216 104 312 8.16e-65 203
MS.gene041475.t1 MTR_3g034180 54.274 234 79 6 3 216 263 488 9.34e-65 208
MS.gene041475.t1 MTR_4g023080 53.881 219 86 6 3 216 145 353 1.96e-63 201
MS.gene041475.t1 MTR_3g103020 53.712 229 85 7 3 217 157 378 3.15e-63 201
MS.gene041475.t1 MTR_4g109280 57.711 201 73 6 20 217 168 359 1.19e-62 199
MS.gene041475.t1 MTR_4g109270 54.751 221 88 5 3 217 150 364 1.36e-62 199
MS.gene041475.t1 MTR_8g040935 56.311 206 77 6 18 217 10 208 3.40e-62 193
MS.gene041475.t1 MTR_4g116330 52.423 227 88 7 3 217 162 380 1.52e-59 191
MS.gene041475.t1 MTR_3g034160 51.073 233 88 5 3 216 149 374 1.97e-59 191
MS.gene041475.t1 MTR_4g118380 55.392 204 71 5 3 191 159 357 2.47e-59 190
MS.gene041475.t1 MTR_4g116320 55.172 203 75 7 17 214 64 255 1.50e-57 182
MS.gene041475.t1 MTR_4g122850 51.528 229 90 8 3 217 143 364 1.63e-57 186
MS.gene041475.t1 MTR_1g055135 49.351 231 97 6 3 217 149 375 2.50e-56 183
MS.gene041475.t1 MTR_4g023090 47.826 230 93 6 3 212 157 379 3.11e-56 183
MS.gene041475.t1 MTR_3g034130 53.846 208 70 5 3 191 149 349 7.45e-56 182
MS.gene041475.t1 MTR_1g055175 51.485 202 83 7 22 217 9 201 1.56e-55 176
MS.gene041475.t1 MTR_4g021785 49.545 220 97 6 3 217 145 355 7.12e-55 179
MS.gene041475.t1 MTR_4g023070 48.133 241 88 9 3 216 139 369 1.09e-54 179
MS.gene041475.t1 MTR_4g118400 54.124 194 65 6 20 211 123 294 2.37e-54 176
MS.gene041475.t1 MTR_4g094365 50.442 226 92 9 3 217 148 364 2.62e-52 172
MS.gene041475.t1 MTR_4g023380 46.444 239 88 8 3 217 157 379 1.02e-51 171
MS.gene041475.t1 MTR_5g097210 50.661 227 84 10 3 212 143 358 1.49e-51 171
MS.gene041475.t1 MTR_4g023120 45.868 242 94 9 3 217 139 370 2.94e-49 164
MS.gene041475.t1 MTR_1g055195 50.000 226 93 9 3 217 262 478 1.72e-48 165
MS.gene041475.t1 MTR_6g463430 47.489 219 92 10 4 217 2 202 2.51e-48 157
MS.gene041475.t1 MTR_8g433030 52.551 196 83 7 20 213 151 338 4.84e-48 160
MS.gene041475.t1 MTR_8g032390 52.551 196 83 7 20 213 184 371 1.21e-47 160
MS.gene041475.t1 MTR_1g055165 52.047 171 71 5 50 217 156 318 2.47e-47 158
MS.gene041475.t1 MTR_5g070080 50.000 224 92 8 3 214 167 382 1.06e-46 158
MS.gene041475.t1 MTR_4g023190 48.430 223 93 9 3 215 147 357 1.44e-46 157
MS.gene041475.t1 MTR_4g023200 47.982 223 95 9 3 215 147 358 2.38e-46 157
MS.gene041475.t1 MTR_4g023150 47.982 223 95 9 3 215 147 358 3.46e-45 154
MS.gene041475.t1 MTR_8g433040 46.320 231 86 10 3 217 156 364 5.72e-44 150
MS.gene041475.t1 MTR_8g032380 46.320 231 86 10 3 217 156 364 5.72e-44 150
MS.gene041475.t1 MTR_4g117530 45.205 219 77 7 3 217 74 253 1.69e-43 146
MS.gene041475.t1 MTR_1g055250 45.366 205 92 7 1 194 49 244 3.06e-43 148
MS.gene041475.t1 MTR_4g118370 46.222 225 80 10 3 212 57 255 3.67e-42 143
MS.gene041475.t1 MTR_4g117750 46.018 226 99 9 4 211 133 353 1.01e-40 145
MS.gene041475.t1 MTR_4g117750 59.633 109 38 4 107 212 373 478 2.64e-30 116
MS.gene041475.t1 MTR_7g079400 41.176 238 110 9 1 217 109 337 5.06e-40 140
MS.gene041475.t1 MTR_5g070088 44.298 228 105 8 3 215 163 383 7.78e-40 140
MS.gene041475.t1 MTR_8g102520 41.453 234 111 9 1 217 98 322 1.84e-39 138
MS.gene041475.t1 MTR_5g070130 42.174 230 110 9 3 214 149 373 1.29e-38 137
MS.gene041475.t1 MTR_4g117870 42.731 227 74 8 3 217 85 267 5.36e-38 133
MS.gene041475.t1 MTR_4g023440 42.793 222 73 7 4 217 157 332 3.83e-37 132
MS.gene041475.t1 MTR_4g116330 42.291 227 72 7 3 217 162 341 2.74e-35 127
MS.gene041475.t1 MTR_4g118360 56.391 133 48 5 88 217 1 126 1.10e-34 120
MS.gene041475.t1 MTR_2g048580 35.586 222 119 8 3 217 138 342 8.95e-31 115
MS.gene041475.t1 MTR_7g079370 36.207 232 116 8 3 213 132 352 9.53e-31 116
MS.gene041475.t1 MTR_4g117430 52.066 121 45 4 3 118 42 154 2.86e-30 109
MS.gene041475.t1 MTR_8g098830 42.105 171 85 6 21 187 34 194 6.92e-30 110
MS.gene041475.t1 MTR_4g121580 44.388 196 62 9 3 185 157 318 2.86e-29 112
MS.gene041475.t1 MTR_5g023000 59.375 96 36 2 123 217 6 99 1.64e-27 100
MS.gene041475.t1 MTR_8g013010 54.032 124 37 5 3 116 196 309 8.12e-27 104
MS.gene041475.t1 MTR_4g122860 39.394 198 79 8 27 217 157 320 6.73e-26 102
MS.gene041475.t1 MTR_7g079450 42.857 147 71 5 73 217 2 137 1.42e-22 89.4
MS.gene041475.t1 MTR_7g079420 37.870 169 93 7 53 217 984 1144 2.36e-22 95.1
MS.gene041475.t1 MTR_4g114700 35.586 222 73 7 3 208 51 218 2.79e-21 88.6
MS.gene041475.t1 MTR_7g117720 54.545 88 35 2 129 212 186 272 1.40e-16 76.6
MS.gene041475.t1 MTR_4g023430 51.190 84 35 2 138 217 141 222 4.47e-16 74.3
MS.gene041475.t1 MTR_6g034720 45.000 100 41 3 3 90 73 170 9.38e-16 73.2
MS.gene041475.t1 MTR_8g013005 63.934 61 19 2 158 217 1 59 4.54e-14 64.7
MS.gene041475.t1 MTR_1g094210 28.283 198 113 9 3 187 195 376 4.12e-12 64.7
MS.gene041475.t1 MTR_4g116340 47.674 86 33 4 3 78 164 247 1.60e-11 62.4
MS.gene041475.t1 MTR_1g094210 27.835 194 111 9 3 183 195 372 7.72e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 51 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAGTTTGATGGAATATA+AGG 0.192370 8.2:+11727293 None:intergenic
GATTGTGGTTTGTGGGTATT+TGG 0.221112 8.2:-11727029 MS.gene041475:CDS
ATCGTAATACAACTGAAATA+TGG 0.303594 8.2:-11726977 MS.gene041475:CDS
GAGTAGGGTCCCTTTGTAAT+GGG 0.322096 8.2:-11727184 MS.gene041475:CDS
TTTGTAATGGGGCTATTCAT+TGG 0.336970 8.2:-11727172 MS.gene041475:CDS
CTAATGATGTCCTTTACATT+TGG 0.354400 8.2:-11726902 MS.gene041475:CDS
CCCTTATATTCCATCAAACT+TGG 0.358101 8.2:-11727294 MS.gene041475:CDS
GGAGTAGGGTCCCTTTGTAA+TGG 0.396390 8.2:-11727185 MS.gene041475:CDS
TTTCCAGATGATTATTATAA+CGG 0.407070 8.2:-11727071 MS.gene041475:CDS
AGAGCATTGCTCCAATTGTA+AGG 0.429098 8.2:-11726778 MS.gene041475:CDS
TAATGGGGCTATTCATTGGT+TGG 0.429474 8.2:-11727168 MS.gene041475:CDS
GACTCTTTGCTTTCACTCCC+CGG 0.434215 8.2:-11726726 MS.gene041475:CDS
TCGTAATACAACTGAAATAT+GGG 0.435341 8.2:-11726976 MS.gene041475:CDS
ACCACGTGAATCCTTACAAT+TGG 0.439030 8.2:+11726767 None:intergenic
AAACCGTTATAATAATCATC+TGG 0.454919 8.2:+11727068 None:intergenic
CCAAGTTTGATGGAATATAA+GGG 0.458503 8.2:+11727294 None:intergenic
TTAAGCTTGCTCATTGCCAC+CGG 0.460768 8.2:+11726707 None:intergenic
ACGTATGGATTGTGGTTTGT+GGG 0.472010 8.2:-11727036 MS.gene041475:CDS
TTTCCTTTCTGTTAAATCAA+AGG 0.476302 8.2:+11727109 None:intergenic
GTTGATGATTATTCCGGAGT+AGG 0.486016 8.2:-11727200 MS.gene041475:CDS
ACTCCGGAATAATCATCAAC+GGG 0.493191 8.2:+11727203 None:intergenic
AGTCCCGTTGATGATTATTC+CGG 0.493361 8.2:-11727206 MS.gene041475:CDS
CTGCTACAAGGGGAGTGATC+AGG 0.499364 8.2:+11727227 None:intergenic
TTTCCTTTGATTTAACAGAA+AGG 0.499674 8.2:-11727112 MS.gene041475:CDS
AACGTATGGATTGTGGTTTG+TGG 0.511387 8.2:-11727037 MS.gene041475:CDS
AATACAAAGTGAATTCGTCT+TGG 0.512787 8.2:-11726944 MS.gene041475:CDS
TGGTGGTAGTACAGAAAGGT+TGG 0.512790 8.2:-11726829 MS.gene041475:CDS
TTGATGATTATTCCGGAGTA+GGG 0.518500 8.2:-11727199 MS.gene041475:CDS
GGTGATATTATTGGAAAAGA+TGG 0.522115 8.2:-11726849 MS.gene041475:CDS
TAAGCTTGCTCATTGCCACC+GGG 0.526590 8.2:+11726708 None:intergenic
CATCAACGGGACTGCTACAA+GGG 0.529706 8.2:+11727216 None:intergenic
ATTACAAAGGGACCCTACTC+CGG 0.531948 8.2:+11727187 None:intergenic
GGGAAAAGTACCAAATGTAA+AGG 0.532919 8.2:+11726892 None:intergenic
ATTTCTCAGTCTATGCGCTA+AGG 0.533303 8.2:-11727003 MS.gene041475:CDS
TCATCAACGGGACTGCTACA+AGG 0.534486 8.2:+11727215 None:intergenic
TCTCATTGAGAGACAATACA+TGG 0.536974 8.2:-11727265 MS.gene041475:CDS
TTTCTCAGTCTATGCGCTAA+GGG 0.541350 8.2:-11727002 MS.gene041475:CDS
ATTTGTGAACGTATGGATTG+TGG 0.541400 8.2:-11727044 MS.gene041475:CDS
ATGAATAGCCCCATTACAAA+GGG 0.555543 8.2:+11727175 None:intergenic
CTGATCATCTCCAAAACCAT+AGG 0.587717 8.2:+11727334 None:intergenic
TACTCCGGAATAATCATCAA+CGG 0.590143 8.2:+11727202 None:intergenic
AATGAATAGCCCCATTACAA+AGG 0.597680 8.2:+11727174 None:intergenic
AAGATGGTGGTAGTACAGAA+AGG 0.602755 8.2:-11726833 MS.gene041475:CDS
AATTGTAAGGATTCACGTGG+TGG 0.626284 8.2:-11726765 MS.gene041475:CDS
AGTAGGGTCCCTTTGTAATG+GGG 0.631345 8.2:-11727183 MS.gene041475:CDS
GATATTATTGGAAAAGATGG+TGG 0.651238 8.2:-11726846 MS.gene041475:CDS
ATCAACGGGACTGCTACAAG+GGG 0.680280 8.2:+11727217 None:intergenic
AAGCTTGCTCATTGCCACCG+GGG 0.691782 8.2:+11726709 None:intergenic
TCCAATTGTAAGGATTCACG+TGG 0.697098 8.2:-11726768 MS.gene041475:CDS
TCTTTGCTTTCACTCCCCGG+TGG 0.705967 8.2:-11726723 MS.gene041475:CDS
TTGGTGAAGTATGATGACAA+TGG 0.748686 8.2:-11726810 MS.gene041475:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCCAGATGATTATTATAA+CGG - chr8.2:11726974-11726993 MS.gene041475:CDS 20.0%
!!! TTTTCGTGATGATTTAAAAA+AGG - chr8.2:11726901-11726920 MS.gene041475:CDS 20.0%
! AAACCGTTATAATAATCATC+TGG + chr8.2:11726980-11726999 None:intergenic 25.0%
! ACAAAAAGTGGTGATATTAT+TGG - chr8.2:11727187-11727206 MS.gene041475:CDS 25.0%
! ATCGTAATACAACTGAAATA+TGG - chr8.2:11727068-11727087 MS.gene041475:CDS 25.0%
! TCGTAATACAACTGAAATAT+GGG - chr8.2:11727069-11727088 MS.gene041475:CDS 25.0%
! TTTCCTTTCTGTTAAATCAA+AGG + chr8.2:11726939-11726958 None:intergenic 25.0%
!! ACTTTTTGTACAACAAATTG+GGG + chr8.2:11727176-11727195 None:intergenic 25.0%
!! TTTCCTTTGATTTAACAGAA+AGG - chr8.2:11726933-11726952 MS.gene041475:CDS 25.0%
!!! CACTTTTTGTACAACAAATT+GGG + chr8.2:11727177-11727196 None:intergenic 25.0%
AATACAAAGTGAATTCGTCT+TGG - chr8.2:11727101-11727120 MS.gene041475:CDS 30.0%
CCAAGTTTGATGGAATATAA+GGG + chr8.2:11726754-11726773 None:intergenic 30.0%
CTAATGATGTCCTTTACATT+TGG - chr8.2:11727143-11727162 MS.gene041475:CDS 30.0%
GATATTATTGGAAAAGATGG+TGG - chr8.2:11727199-11727218 MS.gene041475:CDS 30.0%
GGTGATATTATTGGAAAAGA+TGG - chr8.2:11727196-11727215 MS.gene041475:CDS 30.0%
TCCAAGTTTGATGGAATATA+AGG + chr8.2:11726755-11726774 None:intergenic 30.0%
!! CCAATTTGTTGTACAAAAAG+TGG - chr8.2:11727175-11727194 MS.gene041475:CDS 30.0%
!!! CCACTTTTTGTACAACAAAT+TGG + chr8.2:11727178-11727197 None:intergenic 30.0%
AATGAATAGCCCCATTACAA+AGG + chr8.2:11726874-11726893 None:intergenic 35.0%
ATGAATAGCCCCATTACAAA+GGG + chr8.2:11726873-11726892 None:intergenic 35.0%
CCCTTATATTCCATCAAACT+TGG - chr8.2:11726751-11726770 MS.gene041475:CDS 35.0%
GAGAAAAACTCCAAGTTTGA+TGG + chr8.2:11726764-11726783 None:intergenic 35.0%
TACTCCGGAATAATCATCAA+CGG + chr8.2:11726846-11726865 None:intergenic 35.0%
TCTCATTGAGAGACAATACA+TGG - chr8.2:11726780-11726799 MS.gene041475:CDS 35.0%
TTGGTGAAGTATGATGACAA+TGG - chr8.2:11727235-11727254 MS.gene041475:CDS 35.0%
! ATTTGTGAACGTATGGATTG+TGG - chr8.2:11727001-11727020 MS.gene041475:CDS 35.0%
! TTGATGATTATTCCGGAGTA+GGG - chr8.2:11726846-11726865 MS.gene041475:CDS 35.0%
! TTTGTAATGGGGCTATTCAT+TGG - chr8.2:11726873-11726892 MS.gene041475:CDS 35.0%
!! GGGAAAAGTACCAAATGTAA+AGG + chr8.2:11727156-11727175 None:intergenic 35.0%
!!! CGGTTTTATTTGTGAACGTA+TGG - chr8.2:11726994-11727013 MS.gene041475:CDS 35.0%
AATTGTAAGGATTCACGTGG+TGG - chr8.2:11727280-11727299 MS.gene041475:CDS 40.0%
ACCACGTGAATCCTTACAAT+TGG + chr8.2:11727281-11727300 None:intergenic 40.0%
ACTCCGGAATAATCATCAAC+GGG + chr8.2:11726845-11726864 None:intergenic 40.0%
ATTTCTCAGTCTATGCGCTA+AGG - chr8.2:11727042-11727061 MS.gene041475:CDS 40.0%
CTGATCATCTCCAAAACCAT+AGG + chr8.2:11726714-11726733 None:intergenic 40.0%
TAATGGGGCTATTCATTGGT+TGG - chr8.2:11726877-11726896 MS.gene041475:CDS 40.0%
TCCAATTGTAAGGATTCACG+TGG - chr8.2:11727277-11727296 MS.gene041475:CDS 40.0%
TTTCTCAGTCTATGCGCTAA+GGG - chr8.2:11727043-11727062 MS.gene041475:CDS 40.0%
! AACGTATGGATTGTGGTTTG+TGG - chr8.2:11727008-11727027 MS.gene041475:CDS 40.0%
! AAGATGGTGGTAGTACAGAA+AGG - chr8.2:11727212-11727231 MS.gene041475:CDS 40.0%
! ACGTATGGATTGTGGTTTGT+GGG - chr8.2:11727009-11727028 MS.gene041475:CDS 40.0%
! GATTGTGGTTTGTGGGTATT+TGG - chr8.2:11727016-11727035 MS.gene041475:CDS 40.0%
!! AGAGCATTGCTCCAATTGTA+AGG - chr8.2:11727267-11727286 MS.gene041475:CDS 40.0%
!! AGTCCCGTTGATGATTATTC+CGG - chr8.2:11726839-11726858 MS.gene041475:CDS 40.0%
!! GTTGATGATTATTCCGGAGT+AGG - chr8.2:11726845-11726864 MS.gene041475:CDS 40.0%
AGTAGGGTCCCTTTGTAATG+GGG - chr8.2:11726862-11726881 MS.gene041475:CDS 45.0%
ATTACAAAGGGACCCTACTC+CGG + chr8.2:11726861-11726880 None:intergenic 45.0%
GAGTAGGGTCCCTTTGTAAT+GGG - chr8.2:11726861-11726880 MS.gene041475:CDS 45.0%
TGGTGGTAGTACAGAAAGGT+TGG - chr8.2:11727216-11727235 MS.gene041475:CDS 45.0%
! TTAAGCTTGCTCATTGCCAC+CGG + chr8.2:11727341-11727360 None:intergenic 45.0%
ATCAACGGGACTGCTACAAG+GGG + chr8.2:11726831-11726850 None:intergenic 50.0%
CATCAACGGGACTGCTACAA+GGG + chr8.2:11726832-11726851 None:intergenic 50.0%
GACTCTTTGCTTTCACTCCC+CGG - chr8.2:11727319-11727338 MS.gene041475:CDS 50.0%
GGAGTAGGGTCCCTTTGTAA+TGG - chr8.2:11726860-11726879 MS.gene041475:CDS 50.0%
TCATCAACGGGACTGCTACA+AGG + chr8.2:11726833-11726852 None:intergenic 50.0%
! TAAGCTTGCTCATTGCCACC+GGG + chr8.2:11727340-11727359 None:intergenic 50.0%
CTGCTACAAGGGGAGTGATC+AGG + chr8.2:11726821-11726840 None:intergenic 55.0%
TCTTTGCTTTCACTCCCCGG+TGG - chr8.2:11727322-11727341 MS.gene041475:CDS 55.0%
! AAGCTTGCTCATTGCCACCG+GGG + chr8.2:11727339-11727358 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 11726707 11727360 11726707 ID=MS.gene041475
chr8.2 mRNA 11726707 11727360 11726707 ID=MS.gene041475.t1;Parent=MS.gene041475
chr8.2 exon 11726707 11727360 11726707 ID=MS.gene041475.t1.exon1;Parent=MS.gene041475.t1
chr8.2 CDS 11726707 11727360 11726707 ID=cds.MS.gene041475.t1;Parent=MS.gene041475.t1
Gene Sequence

>MS.gene041475

ATGTCCTATGGTTTTGGAGATGATCAGTCAACAAATCATTACATCCCTTATATTCCATCAAACTTGGAGTTTTTCTCATTGAGAGACAATACATGGAAAGAAATTGAGTGTCCTGATCACTCCCCTTGTAGCAGTCCCGTTGATGATTATTCCGGAGTAGGGTCCCTTTGTAATGGGGCTATTCATTGGTTGGCTTTTCGTGATGATTTAAAAAAGGATGTTATTGTTTCCTTTGATTTAACAGAAAGGAAACTTTTAGATATAGATTTTCCAGATGATTATTATAACGGTTTTATTTGTGAACGTATGGATTGTGGTTTGTGGGTATTTGGAGAATTTCTCAGTCTATGCGCTAAGGGTTATCGTAATACAACTGAAATATGGGTGATGAAAGAATACAAAGTGAATTCGTCTTGGACTAAGACTCATGTTTTTTCTAATGATGTCCTTTACATTTGGTACTTTTCCCCAATTTGTTGTACAAAAAGTGGTGATATTATTGGAAAAGATGGTGGTAGTACAGAAAGGTTGGTGAAGTATGATGACAATGGACAGTTGCTAGAGCATTGCTCCAATTGTAAGGATTCACGTGGTGGATATACTATGTATACAGACTCTTTGCTTTCACTCCCCGGTGGCAATGAGCAAGCTTAA

Protein sequence

>MS.gene041475.t1

MSYGFGDDQSTNHYIPYIPSNLEFFSLRDNTWKEIECPDHSPCSSPVDDYSGVGSLCNGAIHWLAFRDDLKKDVIVSFDLTERKLLDIDFPDDYYNGFICERMDCGLWVFGEFLSLCAKGYRNTTEIWVMKEYKVNSSWTKTHVFSNDVLYIWYFSPICCTKSGDIIGKDGGSTERLVKYDDNGQLLEHCSNCKDSRGGYTMYTDSLLSLPGGNEQA