AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031591

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031591.t1 MTR_4g080200 50.000 360 128 12 1 318 1 350 1.45e-100 300
MS.gene031591.t1 MTR_1g009110 43.432 373 147 16 2 317 13 378 2.84e-72 228
MS.gene031591.t1 MTR_1g022200 41.803 366 155 12 1 317 13 369 3.79e-71 225
MS.gene031591.t1 MTR_1g023400 40.872 367 152 15 1 317 1 352 7.28e-67 213
MS.gene031591.t1 MTR_1g037540 37.778 405 153 16 3 317 5 400 6.02e-60 197
MS.gene031591.t1 MTR_1g022105 35.274 292 127 10 2 275 20 267 3.66e-36 132
MS.gene031591.t1 MTR_8g093280 58.654 104 40 2 3 105 7 108 1.63e-30 111
MS.gene031591.t1 MTR_1g023430 40.299 134 48 3 214 317 41 172 1.06e-19 85.1
MS.gene031591.t1 MTR_2g021150 23.958 288 189 8 3 272 14 289 1.04e-17 83.2
MS.gene031591.t1 MTR_6g007135 27.491 291 179 9 3 272 26 305 1.26e-17 83.2
MS.gene031591.t1 MTR_4g116330 27.178 287 179 11 3 274 23 294 1.36e-16 80.1
MS.gene031591.t1 MTR_3g007410 26.644 289 174 13 6 274 55 325 1.67e-16 79.7
MS.gene031591.t1 MTR_3g007360 26.298 289 175 13 6 274 55 325 2.94e-16 79.3
MS.gene031591.t1 MTR_0667s0010 27.723 303 173 13 3 274 6 293 3.29e-16 79.0
MS.gene031591.t1 MTR_7g016930 27.817 284 158 12 6 260 19 284 3.55e-16 78.2
MS.gene031591.t1 MTR_3g014540 25.517 290 184 10 2 274 28 302 5.16e-16 78.2
MS.gene031591.t1 MTR_2g034700 26.621 293 161 15 6 264 17 289 1.20e-15 77.0
MS.gene031591.t1 MTR_4g133170 23.154 298 180 9 2 274 3 276 1.38e-15 77.0
MS.gene031591.t1 MTR_3g007270 25.084 299 176 11 6 274 50 330 2.11e-15 76.6
MS.gene031591.t1 MTR_2g028140 25.312 320 175 13 3 274 5 308 8.89e-15 74.7
MS.gene031591.t1 MTR_8g063370 26.667 285 166 12 7 260 28 300 1.02e-14 74.7
MS.gene031591.t1 MTR_3g103020 26.736 288 182 11 3 274 20 294 1.16e-14 74.3
MS.gene031591.t1 MTR_3g014570 25.000 320 201 13 2 298 28 331 1.37e-14 73.9
MS.gene031591.t1 MTR_7g070440 27.823 248 152 9 6 234 10 249 2.22e-14 73.6
MS.gene031591.t1 MTR_8g073550 31.217 189 109 6 3 176 30 212 2.64e-14 71.6
MS.gene031591.t1 MTR_4g023090 28.295 258 146 12 3 238 16 256 5.40e-14 72.4
MS.gene031591.t1 MTR_8g013010 27.727 220 134 8 1 203 52 263 5.44e-14 71.6
MS.gene031591.t1 MTR_5g027330 26.115 314 177 11 2 275 39 337 6.09e-14 72.4
MS.gene031591.t1 MTR_3g013770 25.338 296 179 10 3 274 33 310 6.13e-14 72.4
MS.gene031591.t1 MTR_6g053260 26.923 260 159 11 2 241 336 584 6.67e-14 72.8
MS.gene031591.t1 MTR_5g027370 25.723 311 182 10 2 275 28 326 9.95e-14 71.6
MS.gene031591.t1 MTR_4g117850 25.939 293 184 14 2 277 22 298 1.12e-13 71.2
MS.gene031591.t1 MTR_1g007430 29.353 201 100 8 14 176 7 203 1.85e-13 70.5
MS.gene031591.t1 MTR_2g426090 26.885 305 171 12 6 274 9 297 2.07e-13 70.9
MS.gene031591.t1 MTR_3g007430 25.087 287 180 9 6 274 46 315 2.22e-13 70.5
MS.gene031591.t1 MTR_8g046310 29.054 296 159 17 6 267 19 297 2.52e-13 70.5
MS.gene031591.t1 MTR_2g046920 26.800 250 148 9 3 233 24 257 2.63e-13 70.1
MS.gene031591.t1 MTR_4g116340 27.830 212 126 7 3 203 36 231 2.68e-13 68.9
MS.gene031591.t1 MTR_4g116330 30.233 215 126 8 3 203 23 227 2.74e-13 70.1
MS.gene031591.t1 MTR_7g013590 26.148 283 181 9 6 272 39 309 5.18e-13 69.3
MS.gene031591.t1 MTR_3g007330 22.945 292 182 10 2 274 47 314 5.52e-13 69.3
MS.gene031591.t1 MTR_2g028160 24.359 312 184 9 3 274 5 304 8.76e-13 68.9
MS.gene031591.t1 MTR_3g034130 25.817 306 177 13 3 277 9 295 9.78e-13 68.6
MS.gene031591.t1 MTR_2g028130 24.516 310 184 11 3 274 5 302 1.11e-12 68.6
MS.gene031591.t1 MTR_4g094365 28.571 245 144 10 6 233 11 241 1.14e-12 68.2
MS.gene031591.t1 MTR_3g013740 24.422 303 183 14 2 274 43 329 1.41e-12 68.2
MS.gene031591.t1 MTR_5g092850 25.620 242 131 9 6 206 19 252 1.72e-12 67.8
MS.gene031591.t1 MTR_4g070550 23.529 306 192 9 3 274 5 302 1.83e-12 67.8
MS.gene031591.t1 MTR_5g074850 26.667 270 141 12 6 227 38 298 2.48e-12 68.2
MS.gene031591.t1 MTR_2g065860 26.794 209 113 7 6 179 15 218 3.04e-12 67.0
MS.gene031591.t1 MTR_7g066840 29.588 267 139 13 3 235 23 274 4.94e-12 66.6
MS.gene031591.t1 MTR_4g055060 26.667 255 151 10 6 234 33 277 5.22e-12 66.2
MS.gene031591.t1 MTR_2g046900 25.856 263 161 8 3 245 28 276 7.78e-12 65.9
MS.gene031591.t1 MTR_7g066850 30.841 214 121 8 3 202 23 223 7.89e-12 66.2
MS.gene031591.t1 MTR_8g464770 27.586 261 143 10 6 233 15 262 8.87e-12 65.9
MS.gene031591.t1 MTR_3g007300 27.113 284 156 11 2 275 48 290 9.31e-12 65.5
MS.gene031591.t1 MTR_5g070130 25.589 297 181 11 3 274 11 292 1.17e-11 65.5
MS.gene031591.t1 MTR_4g023080 28.629 248 141 13 3 234 9 236 1.21e-11 65.1
MS.gene031591.t1 MTR_5g075370 26.374 273 141 12 6 227 11 274 1.28e-11 65.9
MS.gene031591.t1 MTR_4g109270 25.253 297 183 12 1 277 6 283 1.30e-11 65.1
MS.gene031591.t1 MTR_5g097220 25.172 290 152 12 2 234 12 293 1.80e-11 64.7
MS.gene031591.t1 MTR_4g117780 24.839 310 187 12 2 285 10 299 1.85e-11 64.7
MS.gene031591.t1 MTR_2g426070 25.574 305 178 13 6 274 9 300 1.95e-11 64.7
MS.gene031591.t1 MTR_5g011200 24.916 297 171 16 6 274 20 292 2.21e-11 64.3
MS.gene031591.t1 MTR_3g009240 25.773 291 178 13 2 275 35 304 2.22e-11 64.7
MS.gene031591.t1 MTR_6g017270 27.885 208 124 6 3 188 9 212 2.60e-11 63.9
MS.gene031591.t1 MTR_4g023440 29.091 220 126 8 3 206 18 223 2.79e-11 63.9
MS.gene031591.t1 MTR_2g426020 23.607 305 184 9 3 274 6 294 3.07e-11 64.3
MS.gene031591.t1 MTR_3g011120 27.698 278 143 15 6 245 17 274 3.11e-11 63.9
MS.gene031591.t1 MTR_8g063440 27.660 235 122 11 6 203 15 238 3.14e-11 63.9
MS.gene031591.t1 MTR_3g030350 22.184 293 185 9 2 274 34 303 3.20e-11 63.9
MS.gene031591.t1 MTR_2g026010 24.068 295 189 8 2 274 3 284 3.26e-11 63.9
MS.gene031591.t1 MTR_7g056390 28.223 287 134 15 6 240 21 287 3.46e-11 63.9
MS.gene031591.t1 MTR_4g108820 27.459 244 121 12 3 233 42 242 3.81e-11 63.5
MS.gene031591.t1 MTR_7g066100 27.861 201 121 5 6 183 14 213 3.82e-11 63.9
MS.gene031591.t1 MTR_6g053240 23.964 338 202 14 2 308 27 340 3.84e-11 63.9
MS.gene031591.t1 MTR_4g028660 23.194 263 153 11 6 227 19 273 4.21e-11 63.5
MS.gene031591.t1 MTR_4g027450 23.194 263 153 11 6 227 19 273 4.21e-11 63.5
MS.gene031591.t1 MTR_3g011060 22.846 267 149 8 6 234 31 278 4.63e-11 63.5
MS.gene031591.t1 MTR_1g097330 25.979 281 178 11 3 267 16 282 4.96e-11 63.5
MS.gene031591.t1 MTR_4g109280 23.162 272 189 9 6 267 11 272 5.13e-11 63.2
MS.gene031591.t1 MTR_2g066150 28.235 170 96 6 1 151 10 172 5.62e-11 63.2
MS.gene031591.t1 MTR_4g006840 27.848 158 99 5 6 155 2 152 5.83e-11 62.0
MS.gene031591.t1 MTR_4g030760 28.767 219 122 9 6 206 76 278 6.94e-11 63.2
MS.gene031591.t1 MTR_3g009310 25.385 260 152 10 2 236 41 283 8.96e-11 62.8
MS.gene031591.t1 MTR_4g024890 28.495 186 116 6 6 178 13 194 9.20e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031591.t1 AT5G62510 27.431 288 177 12 6 273 47 322 2.16e-12 67.8
MS.gene031591.t1 AT3G16210 26.378 254 146 12 3 242 5 231 8.02e-12 65.9
MS.gene031591.t1 AT3G07870 24.262 305 179 14 2 272 27 313 2.93e-11 64.3
MS.gene031591.t1 AT2G40925 26.159 302 175 15 6 279 30 311 2.99e-11 64.3

Find 55 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAATTCTTTCTATGTATT+TGG 0.132248 4.3:-29025564 None:intergenic
CTGAAATTTCAATTTGAAAT+TGG 0.150225 4.3:-29026288 None:intergenic
TAGAGAAGATACAATGAATT+TGG 0.191531 4.3:+29025310 None:intergenic
AGATATAATGCTCTTGTTAA+TGG 0.247846 4.3:+29025899 MS.gene031591:CDS
CTCATTAGTTGTTGGGTTCC+AGG 0.262133 4.3:-29025663 None:intergenic
ACATGAGAGAAGTTTCAATT+TGG 0.270140 4.3:-29025501 None:intergenic
AATGAATTTGGGAGCAGATT+TGG 0.275076 4.3:+29025322 None:intergenic
AAGGGTTTCTTGTATGGTTT+TGG 0.278467 4.3:+29025722 MS.gene031591:CDS
CTACAATTGTCCTCTTACTA+TGG 0.290006 4.3:+29025856 MS.gene031591:CDS
AATAAGGCAGCTACTTTGAT+AGG 0.303362 4.3:-29026357 None:intergenic
TGCGGTAAGGGTTTCTTGTA+TGG 0.314365 4.3:+29025716 MS.gene031591:CDS
ATATGTGGTTGCTGGATATA+TGG 0.322647 4.3:+29026110 MS.gene031591:CDS
TGTGATGTCATCGAGATAAA+AGG 0.355570 4.3:+29026049 MS.gene031591:CDS
AAATTAAAGCAGAGTATGTT+TGG 0.374287 4.3:-29025965 None:intergenic
GATGCATCTCATTAGTTGTT+GGG 0.377980 4.3:-29025670 None:intergenic
TTGTCGTGCCAAGTTTCGTT+TGG 0.379973 4.3:-29025632 None:intergenic
CATCTTCTATTCACAAAGTA+AGG 0.385800 4.3:-29025431 None:intergenic
TTTCAATGACTAGAAGAATA+GGG 0.388602 4.3:-29025475 None:intergenic
AGAGAAGATACAATGAATTT+GGG 0.403841 4.3:+29025311 None:intergenic
GTTGCTGGATATATGGGTTA+TGG 0.419237 4.3:+29026117 MS.gene031591:CDS
TCTTTCTATGTATTTGGTAA+AGG 0.419673 4.3:-29025558 None:intergenic
CTGTTTGTATATGTGGTTGC+TGG 0.421883 4.3:+29026102 MS.gene031591:CDS
ATCAAAACTGTTTGTATATG+TGG 0.425769 4.3:+29026095 MS.gene031591:CDS
TGTCGTGCCAAGTTTCGTTT+GGG 0.433353 4.3:-29025631 None:intergenic
GGCACGACAAATTGATTGCC+TGG 0.443235 4.3:+29025645 MS.gene031591:CDS
CAAGAAACCCTTACCGCAAA+AGG 0.446133 4.3:-29025711 None:intergenic
GTAAAGGACTTAATGAGGAT+TGG 0.451064 4.3:-29025542 None:intergenic
TTATTTACCCAAACGAAACT+TGG 0.454752 4.3:+29025624 MS.gene031591:CDS
GGCATACTGTGATGTGCGTT+TGG 0.457236 4.3:-29026019 None:intergenic
TGATGCATCTCATTAGTTGT+TGG 0.474994 4.3:-29025671 None:intergenic
TATGGGTTATGGATCAAAAC+AGG 0.476151 4.3:+29026128 MS.gene031591:CDS
ACAAGAGCATTATATCTTGA+CGG 0.481351 4.3:-29025893 None:intergenic
ATAATCTAAGCACTAAATCC+TGG 0.483289 4.3:+29025825 MS.gene031591:CDS
TTTGGGAGCAGATTTGGAGA+TGG 0.495294 4.3:+29025328 MS.gene031591:CDS
GGGAGCAGATTTGGAGATGG+AGG 0.495402 4.3:+29025331 MS.gene031591:CDS
CTCGATGACATCACAGGAAG+AGG 0.499416 4.3:-29026041 None:intergenic
TTTGATAGGGGAGTTATGCT+TGG 0.501228 4.3:-29026343 None:intergenic
ATAAGGCAGCTACTTTGATA+GGG 0.510964 4.3:-29026356 None:intergenic
ATCGAGATAAAAGGTTCCCT+TGG 0.514742 4.3:+29026058 MS.gene031591:CDS
ATTTGGTAAAGGACTTAATG+AGG 0.524164 4.3:-29025547 None:intergenic
TATGTGGTTGCTGGATATAT+GGG 0.532537 4.3:+29026111 MS.gene031591:CDS
TGATGTGGGACCATAGTAAG+AGG 0.535665 4.3:-29025866 None:intergenic
TATCTTGACGGACGTTGATG+TGG 0.540563 4.3:-29025881 None:intergenic
ATAGAAAGAATTGAATCTTA+CGG 0.546959 4.3:+29025572 MS.gene031591:CDS
GAATCTTACGGTAGTTGCAA+TGG 0.559942 4.3:+29025584 MS.gene031591:CDS
ATCTTGACGGACGTTGATGT+GGG 0.569014 4.3:-29025880 None:intergenic
GATATTGCATGGCATAACCA+AGG 0.585558 4.3:-29026075 None:intergenic
AGATTATAAACTTCAACCAA+AGG 0.599741 4.3:-29025809 None:intergenic
TACAAGTAAAATCATTGATG+AGG 0.601362 4.3:+29025366 MS.gene031591:CDS
GAAACCCTTACCGCAAAAGG+AGG 0.607631 4.3:-29025708 None:intergenic
ATATTGCATGGCATAACCAA+GGG 0.611326 4.3:-29026074 None:intergenic
TCGATGACATCACAGGAAGA+GGG 0.615702 4.3:-29026040 None:intergenic
CCGCAAAAGGAGGGAGCACG+AGG 0.618495 4.3:-29025698 None:intergenic
TAAGGCAGCTACTTTGATAG+GGG 0.632939 4.3:-29026355 None:intergenic
AAACCCTTACCGCAAAAGGA+GGG 0.634888 4.3:-29025707 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGAAAGAATTGAATCTTA+CGG + chr4.3:29025572-29025591 MS.gene031591:CDS 20.0%
!! ATATGATAAATCAACATATC+TGG - chr4.3:29026178-29026197 None:intergenic 20.0%
!! TTCAATTCTTTCTATGTATT+TGG - chr4.3:29025567-29025586 None:intergenic 20.0%
!!! CTGAAATTTCAATTTGAAAT+TGG - chr4.3:29026291-29026310 None:intergenic 20.0%
! AAATTAAAGCAGAGTATGTT+TGG - chr4.3:29025968-29025987 None:intergenic 25.0%
! AGATATAATGCTCTTGTTAA+TGG + chr4.3:29025899-29025918 MS.gene031591:CDS 25.0%
! AGATTATAAACTTCAACCAA+AGG - chr4.3:29025812-29025831 None:intergenic 25.0%
! ATCAAAACTGTTTGTATATG+TGG + chr4.3:29026095-29026114 MS.gene031591:CDS 25.0%
! CATACTCATAAATCCAAAAA+TGG - chr4.3:29026321-29026340 None:intergenic 25.0%
! TACAAGTAAAATCATTGATG+AGG + chr4.3:29025366-29025385 MS.gene031591:CDS 25.0%
! TCAAATGTGTTCAAAAATCA+TGG + chr4.3:29025390-29025409 MS.gene031591:CDS 25.0%
! TCTTTCTATGTATTTGGTAA+AGG - chr4.3:29025561-29025580 None:intergenic 25.0%
! TTTCAATGACTAGAAGAATA+GGG - chr4.3:29025478-29025497 None:intergenic 25.0%
!! ACAATTGTAGATAATTTTCC+AGG - chr4.3:29025846-29025865 None:intergenic 25.0%
!! TTTTCAATGACTAGAAGAAT+AGG - chr4.3:29025479-29025498 None:intergenic 25.0%
!!! ATGTTGATTTATCATATGTG+TGG + chr4.3:29026181-29026200 MS.gene031591:intron 25.0%
ACATGAGAGAAGTTTCAATT+TGG - chr4.3:29025504-29025523 None:intergenic 30.0%
ATAATCTAAGCACTAAATCC+TGG + chr4.3:29025825-29025844 MS.gene031591:CDS 30.0%
ATTTGGTAAAGGACTTAATG+AGG - chr4.3:29025550-29025569 None:intergenic 30.0%
CATCTTCTATTCACAAAGTA+AGG - chr4.3:29025434-29025453 None:intergenic 30.0%
GACAAAAATGCTTTATCCTT+TGG + chr4.3:29025793-29025812 MS.gene031591:CDS 30.0%
TTATTTACCCAAACGAAACT+TGG + chr4.3:29025624-29025643 MS.gene031591:CDS 30.0%
TTTATCATATGTGTGGTCTT+TGG + chr4.3:29026188-29026207 MS.gene031591:intron 30.0%
! ACAGGTGAACTAAAAAGTAT+AGG + chr4.3:29026146-29026165 MS.gene031591:intron 30.0%
! TTTCAGAGCTAAACCATTTT+TGG + chr4.3:29026305-29026324 MS.gene031591:CDS 30.0%
!! ACAAGAGCATTATATCTTGA+CGG - chr4.3:29025896-29025915 None:intergenic 30.0%
!! CAAACAGTTTTGATATTGCA+TGG - chr4.3:29026089-29026108 None:intergenic 30.0%
AATGATTGTGCTGAAATCCT+TGG + chr4.3:29026211-29026230 MS.gene031591:intron 35.0%
ATATGTGGTTGCTGGATATA+TGG + chr4.3:29026110-29026129 MS.gene031591:CDS 35.0%
ATATTGCATGGCATAACCAA+GGG - chr4.3:29026077-29026096 None:intergenic 35.0%
CTACAATTGTCCTCTTACTA+TGG + chr4.3:29025856-29025875 MS.gene031591:CDS 35.0%
GATGCATCTCATTAGTTGTT+GGG - chr4.3:29025673-29025692 None:intergenic 35.0%
GTAAAGGACTTAATGAGGAT+TGG - chr4.3:29025545-29025564 None:intergenic 35.0%
TATGTGGTTGCTGGATATAT+GGG + chr4.3:29026111-29026130 MS.gene031591:CDS 35.0%
TGATGCATCTCATTAGTTGT+TGG - chr4.3:29025674-29025693 None:intergenic 35.0%
TGTGATGTCATCGAGATAAA+AGG + chr4.3:29026049-29026068 MS.gene031591:CDS 35.0%
! AAGGGTTTCTTGTATGGTTT+TGG + chr4.3:29025722-29025741 MS.gene031591:CDS 35.0%
! ACTAGAAGAATAGGGTTTTC+AGG - chr4.3:29025470-29025489 None:intergenic 35.0%
! CTAGAAGAATAGGGTTTTCA+GGG - chr4.3:29025469-29025488 None:intergenic 35.0%
! TATGGGTTATGGATCAAAAC+AGG + chr4.3:29026128-29026147 MS.gene031591:CDS 35.0%
! TTTTATCTCGATGACATCAC+AGG - chr4.3:29026050-29026069 None:intergenic 35.0%
ATCGAGATAAAAGGTTCCCT+TGG + chr4.3:29026058-29026077 MS.gene031591:CDS 40.0%
CTGTTTGTATATGTGGTTGC+TGG + chr4.3:29026102-29026121 MS.gene031591:CDS 40.0%
GAATCTTACGGTAGTTGCAA+TGG + chr4.3:29025584-29025603 MS.gene031591:CDS 40.0%
GATATTGCATGGCATAACCA+AGG - chr4.3:29026078-29026097 None:intergenic 40.0%
TTTGATAGGGGAGTTATGCT+TGG - chr4.3:29026346-29026365 None:intergenic 40.0%
! GTTGCTGGATATATGGGTTA+TGG + chr4.3:29026117-29026136 MS.gene031591:CDS 40.0%
AAACCCTTACCGCAAAAGGA+GGG - chr4.3:29025710-29025729 None:intergenic 45.0%
CAAGAAACCCTTACCGCAAA+AGG - chr4.3:29025714-29025733 None:intergenic 45.0%
CTCATTAGTTGTTGGGTTCC+AGG - chr4.3:29025666-29025685 None:intergenic 45.0%
GATTGTGCTGAAATCCTTGG+AGG + chr4.3:29026214-29026233 MS.gene031591:intron 45.0%
TCGATGACATCACAGGAAGA+GGG - chr4.3:29026043-29026062 None:intergenic 45.0%
TGATGTGGGACCATAGTAAG+AGG - chr4.3:29025869-29025888 None:intergenic 45.0%
TGCGGTAAGGGTTTCTTGTA+TGG + chr4.3:29025716-29025735 MS.gene031591:CDS 45.0%
TGCTGAAATCCTTGGAGGAA+GGG + chr4.3:29026219-29026238 MS.gene031591:intron 45.0%
TGTCGTGCCAAGTTTCGTTT+GGG - chr4.3:29025634-29025653 None:intergenic 45.0%
TTGTCGTGCCAAGTTTCGTT+TGG - chr4.3:29025635-29025654 None:intergenic 45.0%
TTTGGGAGCAGATTTGGAGA+TGG + chr4.3:29025328-29025347 MS.gene031591:CDS 45.0%
! ATCTTGACGGACGTTGATGT+GGG - chr4.3:29025883-29025902 None:intergenic 45.0%
! TATCTTGACGGACGTTGATG+TGG - chr4.3:29025884-29025903 None:intergenic 45.0%
CTCGATGACATCACAGGAAG+AGG - chr4.3:29026044-29026063 None:intergenic 50.0%
GAAACCCTTACCGCAAAAGG+AGG - chr4.3:29025711-29025730 None:intergenic 50.0%
GGCACGACAAATTGATTGCC+TGG + chr4.3:29025645-29025664 MS.gene031591:CDS 50.0%
GGCATACTGTGATGTGCGTT+TGG - chr4.3:29026022-29026041 None:intergenic 50.0%
GTGCTGAAATCCTTGGAGGA+AGG + chr4.3:29026218-29026237 MS.gene031591:intron 50.0%
GAAATCCTTGGAGGAAGGGC+TGG + chr4.3:29026223-29026242 MS.gene031591:intron 55.0%
GGGAGCAGATTTGGAGATGG+AGG + chr4.3:29025331-29025350 MS.gene031591:CDS 55.0%
! GCTCCCTCCTTTTGCGGTAA+GGG + chr4.3:29025704-29025723 MS.gene031591:CDS 55.0%
! TGCTCCCTCCTTTTGCGGTA+AGG + chr4.3:29025703-29025722 MS.gene031591:CDS 55.0%
GTTGTCCAGCCCTTCCTCCA+AGG - chr4.3:29026231-29026250 None:intergenic 60.0%
! CCTCGTGCTCCCTCCTTTTG+CGG + chr4.3:29025698-29025717 MS.gene031591:CDS 60.0%
! CCGCAAAAGGAGGGAGCACG+AGG - chr4.3:29025701-29025720 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 29025323 29026369 29025323 ID=MS.gene031591
chr4.3 mRNA 29025323 29026369 29025323 ID=MS.gene031591.t1;Parent=MS.gene031591
chr4.3 exon 29025323 29026149 29025323 ID=MS.gene031591.t1.exon1;Parent=MS.gene031591.t1
chr4.3 CDS 29025323 29026149 29025323 ID=cds.MS.gene031591.t1;Parent=MS.gene031591.t1
chr4.3 exon 29026240 29026369 29026240 ID=MS.gene031591.t1.exon2;Parent=MS.gene031591.t1
chr4.3 CDS 29026240 29026369 29026240 ID=cds.MS.gene031591.t1;Parent=MS.gene031591.t1
Gene Sequence

>MS.gene031591

ATGAATTTGGGAGCAGATTTGGAGATGGAGGTGTTGTTGCAGCTACAAGTAAAATCATTGATGAGGTTCAAATGTGTTCAAAAATCATGGAACAATCTCATCAAAAGTCCTTACTTTGTGAATAGAAGATGCAAGTTGCTCATCCCTGAAAACCCTATTCTTCTAGTCATTGAAAAATCCAAATTGAAACTTCTCTCATGTGACAACGACAATGAGAAACCAATCCTCATTAAGTCCTTTACCAAATACATAGAAAGAATTGAATCTTACGGTAGTTGCAATGGTGTTTTTTGTCTCAAAGTTATTTACCCAAACGAAACTTGGCACGACAAATTGATTGCCTGGAACCCAACAACTAATGAGATGCATCATATTCCTCGTGCTCCCTCCTTTTGCGGTAAGGGTTTCTTGTATGGTTTTGGTGCTGTTAACGACGACTTCAAAGTTGTCAAACTCAATATCAATGTGACGACAAAAATGCTTTATCCTTTGGTTGAAGTTTATAATCTAAGCACTAAATCCTGGAAAATTATCTACAATTGTCCTCTTACTATGGTCCCACATCAACGTCCGTCAAGATATAATGCTCTTGTTAATGGTGTTTATCATTGTATTACTCGCTCGACACAATATAATAATGCTCCAAACATACTCTGCTTTAATTTTCGCAATAACAAGTTTCAGCAACTTCAAGCTCCAAACGCACATCACAGTATGCCCTCTTCCTGTGATGTCATCGAGATAAAAGGTTCCCTTGGTTATGCCATGCAATATCAAAACTGTTTGTATATGTGGTTGCTGGATATATGGGTTATGGATCAAAACAGGTGAACTAAAAAGTATAGGATTAAACCAGATATGTTGATTTATCATATGTGTGGTCTTTGGAATGATTGTGCTGAAATCCTTGGAGGAAGGGCTGGACAACTACTCGAGTCATATGATCATCATAGAAACAAGCTTCGCCAATTTCAAATTGAAATTTCAGAGCTAAACCATTTTTGGATTTATGAGTATGTGCCAAGCATAACTCCCCTATCAAAGTAG
Protein sequence

>MS.gene031591.t1

MNLGADLEMEVLLQLQVKSLMRFKCVQKSWNNLIKSPYFVNRRCKLLIPENPILLVIEKSKLKLLSCDNDNEKPILIKSFTKYIERIESYGSCNGVFCLKVIYPNETWHDKLIAWNPTTNEMHHIPRAPSFCGKGFLYGFGAVNDDFKVVKLNINVTTKMLYPLVEVYNLSTKSWKIIYNCPLTMVPHQRPSRYNALVNGVYHCITRSTQYNNAPNILCFNFRNNKFQQLQAPNAHHSMPSSCDVIEIKGSLGYAMQYQNCLYMWLLDIWVMDQNRAGQLLESYDHHRNKLRQFQIEISELNHFWIYEYVPSITPLSK